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| 1 | HIF-1α induces VE-cadherin expression and modulates vasculogenic mimicry in esophageal carcinoma cells显示文摘AIM:To investigate whether hypoxia inducible factor(HIF)-1αmodulates vasculogenic mimicry(VM)by upregulating VE-cadherin expression in esophageal squamous cell carcinoma(ESCC).METHODS:Esophageal squamous cancer cell lines Eca109 and TE13 were transfected with plasmids harboring small interfering RNAs targeting HIF-1αor VEcadherin.The proliferation and invasion of esophageal carcinoma cells were detected by MTT and Transwell migration assays.The formation of tubular networks of cells was analyzed by 3D culture in vitro.BALB/c nude mice were used to observe xenograft tumor formation.The relationship between the expression of HIF-1αand VE-cadherin,ephrin A2(Eph A2)and laminin5γ2(LN5γ2)was measured by Western blot and real-time polymerase chain reaction.RESULTS:Knockdown of HIF-1αinhibited cell proliferation(32.3%±6.1%for Eca109 cells and 38.6%±6.8%for TE13 cells,P<0.05).Both Eca109 and TE13cells formed typical tubular networks.The number of tubular networks markedly decreased when HIF-1αor VE-cadherin was knocked down.Expression of VEcadherin,Eph A2 and LN5γ2 was dramatically inhibited,but the expression of matrix metalloproteinase 2 had no obvious change in HIF-1α-silenced cells.Knockdown of VE-cadherin significantly decreased expression of both Eph A2 and LN5γ2(P<0.05),while HIF-1αexpression was unchanged.The time for xenograft tumor formation was 6±1.2 d for Eca109 cells and Eca109cells transfected with HIF-1αNeo control short hairpin RNA(sh RNA)vector,and 8.4±2.1 d for Eca109 cells transfected with an sh RNA against HIF-1α.Knockdown of HIF-1αinhibited vasculogenic mimicry(VM)and tumorigenicity in vivo.CONCLUSION:HIF-1αmay modulate VM in ESCC by regulating VE-cadherin expression,which affects VM formation through Eph A2 and LN5γ2. | Na-Na Tang Hong Zhu Hong-Jie Zhang Wei-Feng Zhang Hai-Lin Jin Lu Wang Pin Wang Gui-Jun He Bo Hao Rui-Hua Shi | 2014 | World Journal of Gastroenterology2014，20，47： | 25 |
| 2 | Fourth C hinese National Consensus Report on the management of H elicobacter pylori infection显示文摘 | Wen Zhong Liu Yong Xie Hong Cheng Nong Hua Lu Fu Lian Hu Wan Dai Zhang Li Ya Zhou Ye Chen Zhi Rong Zeng Chong Wen Wang Shu Dong Xiao Guo Zong Pan Pin Jin Hu | 2013 | Journal of Digestive Diseases2013，，5： | 9 |
| 3 | Banxia Xiexin Decoction(半夏泻心汤)Treats Diabetic Gastroparesis through PLC-IP3-Ca^2+/NO-cGMP-PKG Signal Pathway显示文摘Objective To test the effect of Banxia Xiexin Decoction(半夏泻心汤,BXD)on the contraction and relaxation of gastric smooth muscle(SM)in diabetic gastroparesis(DGP)model rats,and to explore the mechanism of BXD in the prevention and treatment of DGP through experiments of signal pathway both in vivo and in vitro.Methods Sixty Sprague-Dawley rats were divided into 6 groups according to a random number table:control group,model group,high-,medium-and low-dose BXD groups(9.2,4.6 and 1.8 g/(kg·d),respectively),and domperidone group(10 mg/(kg·d)),10 rats per group.DGP model was established initially by a single intraperitoneal injection of streptozotocin(STZ),and was confirmed by recording gastric emptying,intestinal transport velocity and gastric myoelectric activity of rats after 2 months.Each group was treated with a corresponding drug for 4 weeks.The mRNA and protein expressions of phospholipase C(PLC),inositol triphosphate(IP3),neuronal nitric oxide synthase(nNOS),and cyclic guanosine monophosphate(cGMP)dependent protein kinase G(PKG)were detected by reverse transcription-polymerase chain reaction and Western blot,respectively,while nitric oxide(NO)and cGMP expressions were detected by enzyme-linked immunosorbent assay.Gastric tissues were obtained from rats for primary cell culture preparation.Gastric SM cells were treated with 0.8µmol/L of STZ or STZ plus 1,000,500 and 200µg/mL of BXD or STZ plus 2.5µmol/mL of domperidone for 24,48,72 or 96 h,respectively.The length of gastric SM cells and intracellular Ca^2+concentration([Ca^2+]i)before and after BXD treatment was measured.Results Compared with the model group,high-and medium-dose BXD and domperidone significantly increased the expressions of PLC,IP3,NO,nNOS,cGMP and PKG in rat’s gastric tissue(P<0.01).Gastric SM cells treated with BXD showed a time-and dose-dependent increase in cell viability(P<0.01).The treatment with high-and medium-dose BXD and domperidone inhibited the increase in gastric SM cells length and increased[Ca^2+]i compared with the model cells(P<0.01).Conclusions Treatment with high-and medium-dose BXD significantly attenuated STZ-induced experimental DGP in rats.The therapeutic effect of BXD on DGP rats might be associated with the PLC-IP3-Ca^2+/NO-cGMP-PKG signal pathway. | WANG Bin ZENG Ke-wu HONG Zi-fu Tl Gui-xiang WANG Li-yun LU Pin LIU Zhen | 2020 | Chinese Journal of Integrative Medicine2020，26，11： | 8 |
| 4 | Establishment and clinical application value of an automatic diagnosis platform for rectal cancer T-staging based on a deep neural network显示文摘Background:Colorectal cancer is harmful to the patient’s life.The treatment of patients is determined by accurate preoperative staging.Magnetic resonance imaging(MRI)played an important role in the preoperative examination of patients with rectal cancer,and artificial intelligence(AI)in the learning of images made significant achievements in recent years.Introducing AI into MRI recognition,a stable platform for image recognition and judgment can be established in a short period.This study aimed to establish an automatic diagnostic platform for predicting preoperative T staging of rectal cancer through a deep neural network.Methods:A total of 183 rectal cancer patients’data were collected retrospectively as research objects.Faster region-based convolutional neural networks(Faster R-CNN)were used to build the platform.And the platform was evaluated according to the receiver operating characteristic(ROC)curve.Results:An automatic diagnosis platform for T staging of rectal cancer was established through the study of MRI.The areas under the ROC curve(AUC)were 0.99 in the horizontal plane,0.97 in the sagittal plane,and 0.98 in the coronal plane.In the horizontal plane,the AUC of T1 stage was 1,AUC of T2 stage was 1,AUC of T3 stage was 1,AUC of T4 stage was 1.In the coronal plane,AUC of T1 stage was 0.96,AUC of T2 stage was 0.97,AUC of T3 stage was 0.97,AUC of T4 stage was 0.97.In the sagittal plane,AUC of T1 stage was 0.95,AUC of T2 stage was 0.99,AUC of T3 stage was 0.96,and AUC of T4 stage was 1.00.Conclusion:Faster R-CNN AI might be an effective and objective method to build the platform for predicting rectal cancer T-staging.Trial registration:chictr.org.cn:ChiCTR1900023575;http://gffzz62a03ee25e6f4a77hqfkv0fn60w9u696p.ffgz.tsg.suse.edu.cn/showproj.aspx?proj=39665. | Qing-Yao Wu Shang-Long Liu Pin Sun Ying Li Guang-Wei Liu Shi-Song Liu Ji-Lin Hu Tian-Ye Niu Yun Lu | 2021 | Chinese Medical Journal2021，，7： | 4 |
| 5 | Prophylactic Use of Transjugular Intrahepatic Portosystemic Shunt Aids in the Treatment of Refractory Ascites: Metaregression and Trial Sequential Meta-analysis显示文摘 | Ren Pin Chen Xiao Ju Zhu Ge Zhi Ming Huang Xiao Hua Ye Chang Yuan Hu Guang Rong Lu De Yi Lu Igor Longe Phemba | 2014 | Journal of Clinical Gastroenterology2014，，3： | 2 |
| 6 | Ionic Electroactive Polymers Used in Bionic Robots: A Review显示文摘 | Longfei Chang Yanfa Liu Qian Yang Linfeng Yu Jiaqin Liu Zicai Zhu Pin Lu Yucheng Wu Ying Hu | 2018 | Journal of Bionic Engineering2018，15，5： | 2 |
| 7 | A theoretical model for the bending of a laminated beam with SMA fiber embedded layer显示文摘 | Pin Lu F.S. Cui M.J. Tan | 2009 | Composite Structures2009，，4： | 1 |
| 8 | Rheological behavior ofmolecular weight polyethylene semidilute solutions显示文摘 | Ke Quan chen An Qiu zhang Pin Lu | 1989 | Journal of AppliedPolymer Science1989，，38： | 1 |
| 9 | Increased Infiltration of Activated Tumor-Infiltrating Lymphocytes After High Intensity Focused Ul- trasound Ablation of Human Breast Cancer 显示文摘 | LU Pin ZHU Xinqi XU Zelu | 2009 | Surgery2009，145，3： | 1 |
| 10 | Dynamic Properties of Flexural Beams Using a Nonloeal Elasticity Model显示文摘 | Lu Pin Lee H P Lu C | 2006 | Journal of Applied Physics2006，99，7： | 1 |
| 11 | Effect of combined mutations in the enhancer II and basal core promoter of hepatitis B virus on development of hepatocellular carcinoma in Q idong, C hina显示文摘 | Li‐Shuai Qu Jing Zhu Tao‐Tao Liu Xi‐Zhong Shen Tao‐Yang Chen Zheng‐Pin Ni Run‐Zhou Ni Cui‐Hua Lu | 2014 | Hepatol Res2014，，12： | 1 |
| 12 | A novel clustered SPIO nanoplatform with enhanced magnetic resonance T2 relaxation rate for micro-tumor detection and photothermal synergistic therapy显示文摘Construction of micro tumor sensitive theranostic nanoagents that can increase the accuracy of imaging diagnosis and boost the therapeutic efficacy has been demonstrated for a promising approach for diagnosis and treatment of cancer.Herein,we reported a novel super-paramagnetic iron oxide(SPIO)based nanoplatform that possess significantly enhanced magnetic resonance property and photothermal effect for tumor theranostic purpose.This polyethylene glycol with four phenylboronic acid(PEG-B4)/CNTs@porphyrin(ph)/SPIO(BCPS)nanoplatform was simply prepared via integrated SPIO,ph,and a novel dendrimer with PEG liner and four PBA groups(PEG-B4)on the surface of carbon nanotubes(CNTs).Subsequently,a significant T2 relaxation rate enhanced can be achieved by the reduced accessibility of water to SPIO clustering.Moreover,the synergetic enhanced photothermal from BCPS nanoplatform contributed to better photothermal effect for cancer therapy.Furthermore,the targeting ability to sialic acid overexpressed tumor was further introduced from phenylboronic acid from PEG-B4.We showed that BCPS nanoplatform could not only selectively identify solid tumors and detect micro-sized metastatic tumor(1 mm)in the liver,but also effectively ablate tumors in a xenograft model,thereby achieving a complete cure rate of 100%at low laser dose.Our results highlight the potential of BCPS nanoplatform for accurate micro-tumor diagnosis and effective tumor therapy. | Hongwei Lu Yongjing Xu Ruirui Qiao Ziwei Lu Pin Wang Xindan Zhang An Chen Liming Zou Zhongling Wang | 2020 | Nano Research2020，13，8： | 1 |
| 13 | Sequence analysis of the drug?resistant rpoB gene in the Mycobacterium tuberculosisL?form among patients with pneumoconiosis complicated by tuberculosis显示文摘 | Jun Lu Shan Jiang Song Ye Yun Deng Shuai Ma Chao?Pin Li | 2014 | Molecular Medicine Reports2014，，4： | 1 |
| 14 | Titanium Dioxide Nanoparticles Co-doped with Fe3+and Eu3+Ions for Photo-catalysis显示文摘 | Yang Pin Lu Cheng Hua Nanping | 2002 | Materials Letters2002，57，： | 1 |
| 15 | A kinematic analysis of cylindrical ultrasonic micromotors显示文摘 | Pin Lu Kwok Hong Lee Siak Piang Lim Wu Zhong Lin | 2001 | Physical2001，，3： | 1 |
| 16 | Dynamic phenotypic heterogeneity and the evolution of multiple RNA subtypes in hepatocellular carcinoma:the PLANET study显示文摘Intra-tumor heterogeneity(ITH) is a key challenge in cancer treatment, but previous studies have focused mainly on the genomic alterations without exploring phenotypic(transcriptomic and immune)heterogeneity. Using one of the largest prospective surgical cohorts for hepatocellular carcinoma(HCC)with multi-region sampling, we sequenced whole genomes and paired transcriptomes from 67 HCC patients(331 samples). We found that while genomic ITH was rather constant across stages, phenotypic ITH had a very different trajectory and quickly diversified in stage Ⅱ patients. Most strikingly, 30% of patients were found to contain more than one transcriptomic subtype within a single tumor. Such phenotypic ITH was found to be much more informative in predicting patient survival than genomic ITH and explains the poor efficacy of single-target systemic therapies in HCC. Taken together, we not only revealed an unprecedentedly dynamic landscape of phenotypic heterogeneity in HCC, but also highlighted the importance of studying phenotypic evolution across cancer types. | Weiwei Zhai Hannah Lai Neslihan Arife Kaya Jianbin Chen Hechuan Yang Bingxin Lu Jia Qi Lim Siming Ma Sin Chi Chew Khi Pin Chua Jacob Josiah Santiago Alvarez Pauline Jieqi Chen Mei Mei Chang Lingyan Wu Brian K.P.Goh Alexander Yaw-Fui Chung Chung Yip Chan Peng Chung Cheow Ser Yee Lee Juinn Huar Kam Alfred Wei-Chieh Kow Iyer Shridhar Ganpathi Rawisak Chanwat Jidapa Thammasiri Boon Koon Yoong Diana Bee-Lan Ong Vanessa H.de Villa Rouchelle D.Dela Cruz Tracy Jiezhen Loh Wei Keat Wan Zeng Zeng Anders Jacobsen Skanderup Yin Huei Pang Krishnakumar Madhavan Tony Kiat-Hon Lim Glenn Bonney Wei Qiang Leow Valerie Chew Yock Young Dan Wai Leong Tam Han Chong Toh Roger Sik-Yin Foo Pierce Kah-Hoe Chow | 2022 | National Science Review2022，9，3： | 1 |
| 17 | A signaling pathway linking nitric oxide production to hetemtrimeric G protein and hydrogen peroxide regulates extracellhlar calmodulin induction of stomatal closure in Arabidopsis 显示文摘 | LI Jianhua LIU Yinqian LU Pin | 2009 | Plant Physiology2009，150，1： | 1 |
| 18 | Application of nonlocal beam models for carbon nanotubes显示文摘 | Pin Lu H.P. Lee C. Lu P.Q. Zhang | 2007 | International Journal of Solids and Structures2007，，16： | 1 |
| 19 | Ibrutinib Resistance in Chronic Lymphocytic Leukemia显示文摘 | Richard R. Furman Shuhua Cheng Pin Lu Menu Setty Alexendar R. Perez Ailin Guo Joelle Racchumi Guozhou Xu Hao Wu Jiao Ma Susanne M. Steggerda Morton Coleman Christina Leslie Y. Lynn Wang | 2014 | The New England Journal of Medicine2014，，24： | 1 |
| 20 | Application of nonlocal beam models for carbon nanotubes显示文摘 | Pin Lu H.P. Lee C. Lu P.Q. Zhang | 2007 | International Journal of Solids and Structures2007，，16： | 1 |
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