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| 1 | Decreased regional homogeneity in major depression as revealed by resting-state functional magnetic resonance imaging显示文摘Backgroud 功能的成像研究在任务或休息状态期间在消沉在 cortico 边的网络显示反常活动。现在的工作是探索休息状态在消沉与地区性的同质(ReHo ) 显示出的反常自发的活动功能的用 fMRI 的磁性的回声成像(fMRI ).Methods,地区性的大脑活动的差别在在沮丧对健康的参加者放松状态被测量。第一与主要压抑的混乱和 16 控制诊断的十六个参加者在放松状态期间被扫描。一个新奇方法基于 ReHo 被用来在左丘脑越过整个 brain.Results ReHo 检测自发的血液动力学的回答,左时间的脑叶,左小脑的以后的脑叶,并且双边的枕骨的脑叶被发现显著地在放松 depression.Conclusions 的状态与健康控制相比在消沉被减少反常自发的活动在左丘脑,左时间的脑叶,左小脑的以后的脑叶,和双边的枕骨的脑叶存在。并且 ReHo 可以是在在放松消沉的状态理解不同大脑活动的一本潜在的参考书。 | PENG Dai-hui JIANG Kai-da FANG Yi-ru XU Yi-feng SHEN Ting LONG Xiang-yu LIU Jun ZANG Yu-feng | 2011 | Chinese Medical Journal2011，，3： | 28 |
| 2 | Three-field vs two-field lymph node dissection for esophageal cancer:A meta-analysis显示文摘AIM:To assess the effects of 3-field lymphadenectomy for esophageal carcinoma.METHODS:We conducted a computerized literature search of the Pub Med,Cochrane Controlled Trials Register,and EMBASE databases from their inception to present.Randomized controlled trials(RCTs)or observational epidemiological studies(cohort studies)that compared the survival rates and/or postoperative complications between 2-field lymphadenectomy(2FL)and3-field lymphadenectomy(3FL)for esophageal carcinoma with R0 resection were included.Meta-analysis was conducted using published data on 3FL vs 2FL in esophageal carcinoma patients.End points were 1-,3-,and 5-year overall survival rates and postoperativecomplications,including recurrent nerve palsy,anastomosis leak,pulmonary complications,and chylothorax.Subgroup analysis was performed on the involvement of recurrent laryngeal lymph nodes.RESULTS:Two RCTs and 18 observational studies with over 7000 patients were included.There was a clear benefit for 3FL in the 1-(RR=1.16;95%CI:1.09-1.24;P<0.01),3-(RR=1.44;95%CI:1.19-1.75;P<0.01),and 5-year overall survival rates(RR=1.37;95%CI:1.18-1.59;P<0.01).For postoperative complications,3FL was associated with significantly more recurrent nerve palsy(RR=1.43;95%CI:1.28-1.60;P=0.02)and anastomosis leak(RR=1.26;95%CI:1.05-1.52;P=0.09).In contrast,there was no significant difference for pulmonary complications(RR=0.93;95%CI:0.75-1.16,random-effects model;P=0.27)or chylothorax(RR=0.77;95%CI:0.32-1.85;P=0.69).CONCLUSION:This meta-analysis shows that 3FL improves overall survival rate but has more complications.Because of the high heterogeneity among outcomes,definite conclusions are difficult to draw. | Guo-Wei Ma Dong-Rong Situ Qi-Long Ma Hao Long Lan-Jun Zhang Peng Lin Tie-Hua Rong | 2014 | World Journal of Gastroenterology2014，20，47： | 25 |
| 3 | Expediting redox kinetics of sulfur species by atomic-scale electrocatalysts in lithium–sulfur batteries显示文摘Lithium–sulfur(Li–S)batteries have extremely high theoretical energy density that make them as promising systems toward vast practical applications.Expediting redox kinetics of sulfur species is a decisive task to break the kinetic limitation of insulating lithium sulfide/disulfide precipitation/dissolution.Herein,we proposed a porphyrinderived atomic electrocatalyst to exert atomic-efficient electrocatalytic effects on polysulfide intermediates.Quantifying electrocatalytic efficiency of liquid/solid conversion through a potentiostatic intermittent titration technique measurement presents a kinetic understanding of specific phase evolutions imparted by the atomic electrocatalyst.Benefiting from atomically dispersed“lithiophilic”and“sulfiphilic”sites on conductive substrates,the finely designed atomic electrocatalyst endows Li–S cells with remarkable cycling stablity(cyclic decay rate of 0.10%in 300 cycles),excellent rate capability(1035 mAh g−1 at 2 C),and impressive areal capacity(10.9 mAh cm−2 at a sulfur loading of 11.3 mg cm−2).The present work expands atomic electrocatalysts to the Li–S chemistry,deepens kinetic understanding of sulfur species evolution,and encourages application of emerging electrocatalysis in other multielectron/multiphase reaction energy systems. | Bo-Quan Li Long Kong Chang-Xin Zhao Qi Jin Xiao Chen Hong-Jie Peng Jin-Lei Qin Jin-Xiu Chen Hong Yuan Qiang Zhang Jia-Qi Huang | 2019 | InfoMat2019，1，4： | 18 |
| 4 | Advanced energy materials for flexible batteries in energy storage:A review显示文摘Smart energy storage has revolutionized portable electronics and electrical vehicles.The current smart energy storage devices have penetrated into flexible electronic markets at an unprecedented rate.Flexible batteries are key power sources to enable vast flexible devices,which put forward additional requirements,such as bendable,twistable,stretchable,and ultrathin,to adapt mechanical deformation under the working conditions.This review summarizes the recent advances in construction and configuration of flexible batteries and discusses the general metrics to benchmark various flexible batteries with different materials and chemistries.Moreover,we present advanced prototype flexible batteries developed by some companies to afford general envision of the technological status.Lastly,the critical points are summarized in the development of flexible batteries and remaining challenges are also presented for the future design of flexible batteries in practical perspectives. | Long Kong Cheng Tang Hong‐Jie Peng Jia‐Qi Huang Qiang Zhang | 2020 | SmartMat2020，1，1： | 16 |
| 5 | Clinical value of preoperative methylated septin 9 in Chinese colorectal cancer patients显示文摘BACKGROUND The methylated septin 9(mSEPT9) assay was the first blood-based test approved by the United States Food and Drug Administration as a colorectal screening test.However, the diagnostic and prognostic role of preoperative mSEPT9 for colorectal cancer(CRC) in Chinese patients is still unknown.AIM To improve the understanding of diagnostic and prognostic factors, serum m SEPT9 was detected in Chinese CRC patients.METHODS A retrospective analysis of 354 cases, of which 300 had CRC and 54 were normal,was performed in China. Patients' characteristics, treatments, and laboratory data, including age, the date of surgery, Union for International Cancer Control(UICC) stages, distant metastasis(M), and so on, were collected. Methylation levels of SEPT9 were quantified by quantitative, methylation-specific polymerase chain reaction before surgery. In addition, the effects of mSEPT9 on the occurrence and prognosis of 330 CRC cases from The Cancer Genome Atlas(TCGA) database were evaluated using bioinformatics analyses. Potential prognostic factors for overall survival(OS) and progression-free survival(PFS)were evaluated by Kaplan-Meier univariate analysis.RESULTSIn Chinese CRC patients, positive mSEPT9 was strongly associated with advanced UICC stages, deeper invasion by the primary tumor, and more distant metastasis. Methylation levels of SEPT9 were stage-dependent and showed a stepwise increase in UICC stages(I–IV), primary tumor categories(T1–T4),regional node categories(N0–N2), and distant metastasis categories(M0–M1).The patients with positive mSEPT9 showed a tendency toward lower PFS. After analyzing TCGA clinical data, the high mSEPT9 group was found to be obviously correlated only with more distant metastasis. The patients with high mSEPT9 levels showed a tendency toward lower OS. Besides, nine meaningful mSEPT9 sites were found to provide guidance for the follow-up studies.CONCLUSION MSEPT9 analysis may add valuable information to current tumor staging. Serum m SEPT9 in Chinese CRC patients appears to offer promising novel prognostic markers and might be considered for monitoring CRC recurrence. | Xue Yang Zhi-Jie Xu Xi Chen Shuang-Shuang Zeng Long Qian Jie Wei Mei Peng Xiang Wang Wan-Li Liu Hong-Ying Ma Zhi-Cheng Gong Yuan-Liang Yan | 2019 | World Journal of Gastroenterology2019，25，17： | 16 |
| 6 | Review of nanostructured current collectors in lithium-sulfur batteries显示文摘 | Long Kong Hong-Jie Peng Jia-Qi Huang Qiang Zhang | 2017 | Nano Research2017，10，12： | 13 |
| 7 | Recurrent atrial tachycardia and atrial fribrillation after circumferential pulmonary vein ablation:What’s the difference?显示文摘Background Recurrent atrial tachyarrhythmia (ATa) after circumferential pulmonary vein ablation (CPVA) includes atrial tachycardia (AT) and atrial fribrillation (AF). However, whether there are some differences in clinical course and mechanisms between the recurrent AT and the recurrent AF remained unclear. This study was conducted to investigate the incidence, mechanism, clinical course of the recurrent AT and AF in patients under CPVA.Methods One hundred and thirty consecutive patients (M/F=95/35) with highly symptomatic and multiple antiarrhythmic drugs (AADs) refractory paroxysmal (n=91) or persistent (n=39) AF were included. The ablation protocol consisted solely of two continuous circular lesions around the ipsilateral pulmonary veins (PV) guided by CARTO system. The endpoint of CPVA is PV isolation. For patients with recurrent ATa within 2 months after the initial procedure, cardioversion with direct current was attempted if the ATa lasted for more than 24 hours. A repeat ablation procedure was performed only for patients with AADs refractory recurrent ATa and at least followed up for 2 months after the initial procedure.Results Within 2 months after the initial procedure, 52 patients (40.0%) had experienced episodes of symptomatic recurrent ATa. Among them, 23 patients (44.2%) with recurred AT alone (AT group), 14 patients (26.9%) with recurred AF alone (AF group), and 15 patients (28.8%) with recurred AT and AF (AT plus AF group). The delayed cure rate (65.2%) in AT group was significant higher than that in AF group (21.4%, P<0.05) and AF plus AT group (26.7%, P<0.05). A repeat ablation was performed in 21 patients, including 6 patients with recurrent AT alone, 8 patients with recurrent AF alone, and 7 patients with recurrent AF plus AT. The mean number of PV gaps was 1.2±0.4 in AT group, which was significantly lower than that in AF group (2.6±0.7, P<0.05) and AF plus AT group (2.0±0.6, P<0.05). Delayed cure rate and number of PV gaps between AF group and AF plus AT group were comparable (P>0.05).Conclusions Present study indicates that recurrent AT and AF after CPVA have the different clinical course and different electrophysiological findings during repeat procedure as follows: ⑴After CPVA, spontaneous resolution of recurrent ATa was mainly found in patients with recurrent AT alone (about two thirds patients). ⑵The type of recurrent ATa after CPVA is associated with the number of PV gaps. | LIU Xing-peng LONG De-yong DONG Jian-zeng LIU Xiao-qing FANG Dong-ping HAO Peng MA Chang-sheng | 2005 | Chinese Medical Journal2005，，21： | 13 |
| 8 | Rapid Detection and Identification of Infectious Pathogens Based on High-throughput Sequencing显示文摘 | Pei-Xiang Ni Xin Ding Yin-Xin Zhang Xue Yao Rui-Xue Sun Peng Wang Yan-Ping Gong Jia-Li Zhou Dong-Fang Li Hong-Long WO Xin Yi Ling Yang Yun Long | 2015 | Chinese Medical Journal2015，，7： | 13 |
| 9 | Diagnostic and Predictive Levels of Calcium-binding Protein A8 and Tumor Necrosis Factor Receptor-associated Factor 6 in Sepsis-associated Encephalopathy: A Prospective Observational Study显示文摘 | Li-Na Zhang Xiao-Hong Wang Long Wu Li Huang Chun-Guang Zhao Qian-Yi Peng Yu-Hang Ai | 2016 | Chinese Medical Journal2016，，14： | 12 |
| 10 | Effect of slurry conditioning on flocculant-aided filtration of coal tailings studied by low-field nuclear magnetic resonance and X-ray micro-tomography显示文摘The present work aimed to study the effect of slurry conditioning on flocculant-aided filtration of coal tailings by the analysis of filtration kinetics and filter cake structure.Laboratory filtration tests of the coal tailings showed that both the shear rate and agitation time have significant effects on filtration rate and cake moisture.Moderate agitation at the shear rate of 92 s^-1 was favorable for fast filtration,but high cake moisture was encountered.The low-field 1H nuclear magnetic resonance(NMR)measurements of the filter cake showed that the slurry conditioning has a significant effect on the residual water in large pores and a negligible effect on the residual water in small pores.The X-ray micro-tomography(XRM)measurements indicated that the filter cake formed at the shear rate of 92 s^-1 has more macro-pores and higher porosity than that formed at the shear rate of 53 s1,hence more residual water was entrapped in filter cake.The slurry conditioning in the presence of flocculant will change the structure of filter cake and affect the filtration performance.There was a paradox between fast filtration rate and low filter cake moisture.The findings enable better understanding of the effect of slurry conditioning on flocculant-aided filtration of coal tailings. | Pengfei Hu Long Liang Guangyuan Xie Shaoqi Zhou Yaoli Peng | 2020 | International Journal of Mining Science and Technology2020，30，6： | 12 |
| 11 | The enhanced X-ray Timing and Polarimetry mission—eXTP显示文摘In this paper we present the enhanced X-ray Timing and Polarimetry mission—eXTP. eXTP is a space science mission designed to study fundamental physics under extreme conditions of density, gravity and magnetism. The mission aims at determining the equation of state of matter at supra-nuclear density, measuring effects of QED, and understanding the dynamics of matter in strong-field gravity. In addition to investigating fundamental physics, eXTP will be a very powerful observatory for astrophysics that will provide observations of unprecedented quality on a variety of galactic and extragalactic objects. In particular, its wide field monitoring capabilities will be highly instrumental to detect the electro-magnetic counterparts of gravitational wave sources.The paper provides a detailed description of:(1) the technological and technical aspects, and the expected performance of the instruments of the scientific payload;(2) the elements and functions of the mission, from the spacecraft to the ground segment. | ShuangNan Zhang Andrea Santangelo Marco Feroci YuPeng Xu FangJun Lu Yong Chen Hua Feng Shu Zhang Sφren Brandt Margarita Hernanz Luca Baldini Enrico Bozzo Riccardo Campana Alessandra De Rosa YongWei Dong Yuri Evangelista Vladimir Karas Norbert Meidinger Aline Meuris Kirpal Nandra Teng Pan Giovanni Pareschi Piotr Orleanski QiuShi Huang Stephane Schanne Giorgia Sironi Daniele Spiga Jiri Svoboda Gianpiero Tagliaferri Christoph Tenzer Andrea Vacchi Silvia Zane Dave Walton ZhanShan Wang Berend Winter Xin Wu Jean J.M.in't Zand Mahdi Ahangarianabhari Giovanni Ambrosi Filippo Ambrosino Marco Barbera Stefano Basso Jörg Bayer Ronaldo Bellazzini Pierluigi Bellutti Bruna Bertucci Giuseppe Bertuccio Giacomo Borghi XueLei Cao Franck Cadoux Francesco Ceraudo TianXiang Chen Yu Peng Chen Jerome Chevenez Marta Civitani Wei Cui WeiWei Cui Thomas Dauser Ettore Del Monte Sergio Di Cosimo Sebastian Diebold Victor Doroshenko Michal Dovciak YuanYuan Du Lorenzo Ducci QingMei Fan Yannick Favre Fabio Fuschino JoséLuis Ga'lvez Min Gao MingYu Ge Olivier Gevin Marco Grassi QuanYing Gu YuDong Gu DaWei Han Bin Hong Wei Hu Long Ji ShuMei Jia WeiChun Jiang Thomas Kennedy Ingo Kreykenbohm Irfan Kuvvetli Claudio Labanti Luca Latronico Gang Li MaoShun Li Xian Li Wei Li ZhengWei Li Olivier Limousin HongWei Liu XiaoJing Liu Bo Lu Tao Luo Daniele Macera Piero Malcovati Adrian Martindale Malgorzata Michalska Bin Meng Massimo Minuti Alfredo Morbidini Fabio Muleri Stephane Paltani Emanuele Perinati Antonino Picciotto Claudio Piemonte JinLu Qu Alexandre Rachevski Irina Rashevskaya Jerome Rodriguez Thomas Schanz ZhengXiang Shen LiZhi Sheng JiangBo Song LiMing Song Carmelo Sgro Liang Sun Ying Tan Phil Uttley Bo Wang DianLong Wang GuoFeng Wang Juan Wang LangPing Wang YuSa Wang Anna L.Watts XiangYang Wen Jörn Wilms ShaoLin Xiong JiaWei Yang Sheng Yang YanJi Yang Nian Yu WenDa Zhang Gianluigi Zampa Nicola Zampa Andrzej A.Zdziarski AiMei Zhang ChengMo Zhang Fan Zhang Long Zhang Tong Zhang Yi Zhang XiaoLi Zhang ZiLiang Zhang BaoSheng Zhao ShiJie Zheng Yu Peng Zhou Nicola Zorzi J.Frans Zwart | 2019 | Science China(Physics,Mechanics & Astronomy)2019，62，2： | 11 |
| 12 | Inheritance and molecular characterization of resistance to AHAS- inhibiting herbicides in rapeseed显示文摘Rapeseed is a very important oil crop in China;however,its production is challenging due to the absence of effective weed management strategies.This is predominantly because of a shortage of herbicide resistance genes.Acetohydroxyacid synthase(AHAS) herbicides inhibit AHAS,a key enzyme involved in branched-chain amino acid synthesis that is required for plant growth.A rapeseed line designated M342 with AHAS herbicide resistance was developed through seed mutagenesis and was studied to assess the level and mode of inheritance of the resistance and to identify the molecular basis of resistance.M342 possessed a high level of cross-resistance to sulfonylureas(SUs) and imidazolinones(IMIs).This resistance was due to AHAS insensitivity to these herbicides and was inherited as a dominant trait conferred by a single nuclear-encoded gene.Molecular analysis revealed the presence of a Trp574 Leu mutation in M342,and an allele-specific cleaved amplified polymorphic sequence(AS-CAPS) marker was developed and cosegregated with herbicide resistance in the F2,BC1,and BC2 populations.This mutation altered the transcript levels of Bn AHAS1 and Bn AHAS3 in M342 compared with those in the wild type,but it did not affect the agronomic or quality traits.The simple genetic inheritance of this mutation and the availability of the cleaved amplified polymorphic sequence(CAPS) marker and herbicide resistance gene should facilitate the development of herbicide-resistant rapeseed cultivars for effective weed control in China. | HU Mao-long PU Hui-ming GAO Jian-qin LONG Wei-hua CHEN Feng ZHOU Xiao-ying ZHANG Wei PENG Qi CHEN Song ZHANG Jie-fu | 2017 | Journal of Integrative Agriculture2017，16，11： | 11 |
| 13 | Casticin induces caspase-mediated apoptosis via activation of mitochondrial pathway and upregulation of DR5 in human lung cancer cells显示文摘Objective:To assess if casticin induces caspase-mediated apoptosis via activation of mitochondrial pathway and upregulation of DR5 in human lung cancer ceils.Methods:Human non-small-cell lung carcinoma cell lines H460,AS49 and H157 were cultured in vitro.The cytotoxic activities were determined using MTT assay.The apoptotic cells death was examined by flow cytometry using PI staining and DMA agarose gel electrophoresis.The activities of caspase-3, -8 and -9 were measured via ELISA.Cellular fractionation was determined by flow cytometry to assess release of cytochrome c and the mitochondrial transmembrane potential.Bcl-2/Bcl-XL/ XIAP/Bid/ DR5 and DR4 proteins were analyzed using western blot.Results:The concentrations required for a 50%decrease in cell growth(IC50) ranged from 1.8 to 3.2 Jt M.Casticin induced rapid apoptosis and triggered a series of effects associated with apoptosis by way of mitochondrial pathway,including the depolarization of the mitochondrial membrane,release of cytochrome c from mitochondria,activation of procaspase-9 and -3,and increase of DNA fragments.Moreover, the pan caspase inhibitor zVAD-FMK and the caspase-3 inhibitor zOEVD-FMK suppressed casticin-induced apoptosis.In addition,casticin induced XIAP and Bcl-XL down-regulation, Bax upregulation and Bid clearage.In H157 cell line,casticin increased expression of DRS at protein levels but not affect the expression of DR4.The prelreatmenl with DR5/Fc chimera protein effectively attenuated casticin-induced apoptosis in H157 cells.No correlation was found between cell sensitivity to casticin and that to p53 status,suggesting that casticin induce a p53- independent apoptosis.Conclusions:Our results demonstrate that casticin induces caspase-mediated apoptosis via activation of mitochondrial pathway and upregulation of DRS in human lung cancer cells. | Yuan Zhou Yi Peng Qi-Qi Mao Xia Li Ming-Wu Chen Ting Su Li Tian Nai-Quan Mao Ling-Zhi Long Mei-Fang Quan Fei Liu Su-Fang Zhou Yong-Xiang Zhao | 2013 | Asian Pacific Journal of Tropical Medicine2013，6，5： | 11 |
| 14 | Grey GM(1,1) Model with Function-Transfer Method for Wear Trend Prediction and its Application显示文摘Trend forecasting is an important aspect in fault diagnosis and work state supervision. The principle, where Grey theory is applied in fault forecasting, is that the forecast system is considered as a Grey system; the existing known information is used to infer the unknown information’s character, state and development trend in a fault pattern, and to make possible forecasting and decisions for future development. It involves the whitenization of a Grey process. But the traditional equal time interval Grey GM (1,1) model requires equal interval data and needs to bring about accumulating addition generation and reversion calculations. Its calculation is very complex. However, the non equal interval Grey GM (1,1) model decreases the condition of the primitive data when establishing a model, but its requirement is still higher and the data were pre processed. The abrasion primitive data of plant could not always satisfy these modeling requirements. Therefore, it establishes a division method suited for general data modeling and estimating parameters of GM (1,1), the standard error coefficient that was applied to judge accuracy height of the model was put forward; further, the function transform to forecast plant abrasion trend and assess GM (1,1) parameter was established. These two models need not pre process the primitive data. It is not only suited for equal interval data modeling, but also for non equal interval data modeling. Its calculation is simple and convenient to use. The oil spectrum analysis acted as an example. The two GM (1,1) models put forward in this paper and the new information model and its comprehensive usage were investigated. The example shows that the two models are simple and practical, and worth expanding and applying in plant fault diagnosis. | LUO You xin 1 , PENG Zhu 2 , ZHANG Long ting 1 , GUO Hui xin 1 , CAI An hui 1 1Department of Mechanical Engineering, Changde Teachers University, Changde 415003, P.R. China 2 Engineering Technology Board, Changsha Cigare | 2001 | International Journal of Plant Engineering and Management2001，6，4： | 11 |
| 15 | Super-resolution dipole orientation mapping via polarization demodulation显示文摘Fluorescence polarization microscopy(FPM)aims to detect the dipole orientation of fluorophores and to resolve structural information for labeled organelles via wide-field or confocal microscopy.Conventional FPM often suffers from the presence of a large number of molecules within the diffraction-limited volume,with averaged fluorescence polarization collected from a group of dipoles with different orientations.Here,we apply sparse deconvolution and least-squares estimation to fluorescence polarization modulation data and demonstrate a super-resolution dipole orientation mapping(SDOM)method that resolves the effective dipole orientation from a much smaller number of fluorescent molecules within a sub-diffraction focal area.We further apply this method to resolve structural details in both fixed and live cells.For the first time,we show that different borders of a dendritic spine neck exhibit a heterogeneous distribution of dipole orientation.Furthermore,we illustrate that the dipole is always perpendicular to the direction of actin filaments in mammalian kidney cells and radially distributed in the hourglass structure of the septin protein under specific labelling.The accuracy of the dipole orientation can be further mapped using the orientation uniform factor,which shows the superiority of SDOM compared with its wide-field counterpart as the number of molecules is decreased within the smaller focal area.Using the inherent feature of the orientation dipole,the SDOM technique,with its fast imaging speed(at sub-second scale),can be applied to a broad range of fluorescently labeled biological systems to simultaneously resolve the valuable dipole orientation information with super-resolution imaging. | Karl Zhanghao Long Chen Xu-San Yang Miao-Yan Wang Zhen-Li Jing Hong-Bin Han Michael Q Zhang Dayong Jin Jun-Tao Gao Peng Xi | 2016 | Light(Science & Applications)2016，5，1： | 11 |
| 16 | Decrease of GSK3βSer-9 Phosphorylation Induced Osteoblast Apoptosis in Rat Osteoarthritis Model显示文摘Nowadays,the cumulative intake of glucocorticoids has become the most common pathogenic factor for non-traumatic osteonecrosis of the femoral head(ONFH).Apoptosis of osteoblasts is considered as the main reason of ONFH at the molecular level.Glycogen synthase kinase 3β(GSK3β)is an important regulator of cellular differentiation and apoptosis pathway,which can modulate the balance between osteoblasts and osteoclasts.Several studies have reported about its function in osteoporosis,but little is known about it in osteonecrosis.In our study,lipopolysaccharide and methylprednisolone were utilized to establish a rat ONFH model.The phosphorylation of GSK3βSer-9 was decreased in the model.Western blotting examination ofβ-catenin,Bcl-2,Bax and caspase-3 revealed that the osteoblasts were apoptotic.In dexamethasone(Dex)-incubated primary osteoblasts,the expression profile of GSK3βphosphorylation and apoptotic factors were consistent with those in the rat ONFH model.To further investigate the regulation of osteonecrosis caused by GSK3β,the expression and function of GSK3βwere inhibited in Dex-incubated primary osteoblasts.The knockdown of GSK3βby siRNA decreased the expression of Bax and cleaved caspase-3,but increased Bcl-2 andβ-catenin.On the other hand,selective inhibition of GSK3βfunction by LiCl counteracted the activation of caspase-3 induced by Dex.Our work is the first study about the GSK3P phosphorylation in ONFH,and provides evidence for further therapeutic methods. | Shuang DENG Zhi-gang NIE Pu-ji PENG Yang LIU Sai XING Lin-sheng LONG Hao PENG | 2019 | Current Medical Science2019，39，1： | 10 |
| 17 | The resurgent landscape of xenotransplantation of pig organs in nonhuman primates显示文摘Organ shortage is a major bottleneck in allotransplantation and causes many wait-listed patients to die or become too sick for transplantation.Genetically engineered pigs have been discussed as a potential alternative to allogeneic donor organs.Although xenotransplantation of pig-derived organs in nonhuman primates(NHPs)has shown sequential advances in recent years,there are still underlying problems that need to be completely addressed before clinical applications,including(i)acute humoral xenograft rejection;(ii)acute cellular rejection;(iii)dysregulation of coagulation and inflammation;(iv)physiological incompatibility;and(v)cross-species infection.Moreover,various genetic modifications to the pig donor need to be fully characterized,with the aim of identifying the ideal transgene combination for upcoming clinical trials.In addition,suitable pretransplant screening methods need to be confirmed for optimal donor-recipient matching,ensuring a good outcome from xenotransplantation.Herein,we summarize the understanding of organ xenotransplantation in pigs-to-NHPs and highlight the current status and recent progress in extending the survival time of pig xenografts and recipients.We also discuss practical strategies for overcoming the obstacles to xenotransplantation mentioned above to further advance transplantation of pig organs in the clinic. | Xuan Zhang Quancheng Wang Jingjun Zhao Xiao Li Wei Peng Zhaoxu Yang Zhibin Lin Long Yang Rui Ding Kaishan Tao Kefeng Dou | 2021 | Science China(Life Sciences)2021，64，5： | 10 |
| 18 | Carcinogenic potential of duodenal reflux juice from patients with long-standing postgastrectomy显示文摘AIM To determine, whether study on thecarcinogenic potential of reflux juice frompatients with remote gastrectomy could clarifythe inherent relationship between duodenalreflux and gastric stump cancer.METHODS A total of 37 reflux juice samples(13 Billroth T, 24 Billroth Ⅱ ) were employed inthe present study. A two- stage transformationassay using BALB/c 3T3 cells was carried out totest the initiating or promoting activity of thesesamples.RESULTS Two of 18 (11.1%) reflux samplesexerted initiating activities, whereas 9/19(47.4%) samples enhanced the MNNG-initiafingcell transformation, suggesting the duodenalreflux juice might more frequently possess thetumor-promoter activity (P = 0. 029). In addition,there was no difference in initiating activities ofthe samples irrespective of surgical procedures(P=0.488), while Billroth Ⅱ samples exhibitedstronger tumor-promoter activity than Billroth Ⅰsamples (P = 0.027). Furthermore, the promoteractivities were well correlated with thehistological changes of the stomas (rs = 0.625,P = 0.004), but neither their cytotoxicities norinitiating activities had this correlation( Probabilities were 0.523 and 0.085,respectively).CONCLUSION The duodenal reflux juice frompatients with remote postgastrectomy did havecarcinogenic potential, and suggested thattumor-promoting activity should principallyaccount for the high incidence of gastric cancerin gastrectomy patients. In contrast, it isdifficult to explain the high stump-cancerincidence with the 'N-nitroso compounds'theory-a popular theory for the intact stomachcarcinogenesis, and it seemed to be justified tofocus chemoprevention of this cancer on thetumor-promoting potential of reflux juice. | Zhe Fu Ma~1 Zhong Yu Wang~1 Jun Ran Zhang~2 Peng Gong~1 Hai Long Chen~1 ~1Departrnent of General Surgery,First Hospital,Dalian Medical University,Dalian 116011,China ~2Department of Radiation Oncology,Massachusetts General Hospital and Harvard Medical School,Charlestown,MA 02129,USADr.Zhe Fu Ma graduated from Shanxi Medical College in 1993,nowPh.D.,M.D.in Department of Surgery,Dalian Medical University,majoring gastroenteric cancer,having 4 papers published.Correspondence to:Dr.Zhe Fu Ma.Department of General Surgery,First Hospital,Dalian Medical University,Dalian 116011,China Tel.0086-411-4720334 | 2001 | World Journal of Gastroenterology2001，7，3： | 9 |
| 19 | Combination of mild therapeutic hypothermia and adipose-derived stem cells for ischemic brain injury显示文摘Mild therapeutic hypothermia has been shown to mitigate cerebral ischemia, reduce cerebral edema, and improve the prognosis of patients with cerebral ischemia. Adipose-derived stem cell-based therapy can decrease neuronal death and infiltration of inflammatory cells, exerting a neuroprotective effect. We hypothesized that the combination of mild therapeutic hypothermia and adipose-derived stem cells would be neuroprotective for treatment of stroke. A rat model of transient middle cerebral artery occlusion was established using the nylon monofilament method. Mild therapeutic hypothermia(33°C) was induced after 2 hours of ischemia. Adipose-derived stem cells were administered through the femoral vein during reperfusion. The severity of neurological dysfunction was measured by a modified Neurological Severity Score Scaling System. The area of the infarct lesion was determined by 2,3,5-triphenyltetrazolium chloride staining. Apoptotic neurons were detected by terminal deoxynucleotidyl transferase-mediated d UTP-biotin nick end labeling(TUNEL) staining. The regeneration of microvessels and changes in the glial scar were detected by immunofluorescence staining. The inflammatory responses after ischemic brain injury were evaluated by in situ staining using markers of inflammatory cells. The expression of inflammatory cytokines was measured by reverse transcription-polymerase chain reaction. Compared with mild therapeutic hypothermia or adipose-derived stem cell treatment alone, their combination substantially improved neurological deficits and decreased infarct size. They synergistically reduced the number of TUNEL-positive cells and glial fibrillary acidic protein expression, increased vascular endothelial growth factor levels, effectively reduced inflammatory cell infiltration and down-regulated the m RNA expression of the proinflammatory cytokines interleukin-1β, tumor necrosis factor-α and interleukin-6. Our findings indicate that combined treatment is a better approach for treating stroke compared with mild therapeutic hypothermia or adipose-derived stem cells alone. | Kai Zhao Rui Li Sheng Bi Yu Li Long Liu Yu-Long Jia Peng Han Chang-Cong Gu Xi-Ze Guo Wan-Ping Zhang Chun Wang Chun-Ying Pei Lin-Lu Tian Li-Xian Li | 2018 | Neural Regeneration Research2018，13，10： | 9 |
| 20 | Effects of yeast and yeast cell wall polysaccharides supplementation on beef cattle growth performance, rumen microbial populations and lipopolysaccharides production显示文摘This experiment was conducted to investigate the effects of live yeast and yeast cell wall polysaccharides on growth performance,rumen function and plasma lipopolysaccharides(LPS) content and immunity parameters of beef cattle.Forty Qinchuan cattle were randomly assigned to one of four treatments with 10 replicates in each treatment.The dietary treatments were: control diet(CTR),CTR supplemented with 1 g live yeast(2×10^10 live cell g^-1 per cattle per day(YST1),CTR supplemented with 2 g live yeast per cattle per day(YST2) and CTR supplemented with 20 g of yeast cell wall polysaccharides(30.0%≤β-glucan≤35.0%,and 28.0%≤mannanoligosaccharide≤32.0%) per cattle per day(YCW).The average daily gain was higher(P=0.023) and feed conversion ratio was lower(P=0.042) for the YST2 than the CTR.The digestibility of neutral detergent fiber(P=0.039) and acid detergent fiber(P=0.016) were higher in yeast supplemented groups.The acetic acid:propionic acid of the YST2 was lower compared with the CTR(P=0.033).Plasma LPS(P=0.032),acute phase protein haptoglobin(P=0.033),plasma amyloid A(P=0.015) and histamine(P=0.038) were lower in the YST2 compared with the CTR.The copies of fibrolytic microbial populations such as Fibrobacter succinogenes S85,Ruminococcus albus 7 and Ruminococcus flavefaciens FD-1 of the YST2 were higher(P<0.001),while the copies of typical lactate producing bacteria Streptococcus bovis JB1 was lower(P<0.001) compared with the CTR.Little differences were observed between the CTR,YST1 and YCW in growth performance,ruminal fermentation characteristics,microbial populations,immunity indices and total tract nutrient digestibility.It is concluded that the YST2 could promote fibrolytic microbial populations,decrease starch-utilizing bacteria,reduce LPS production in the rumen and LPS absorption into plasma and decrease inflammatory parameters,which can lead to an improvement in growth performance in beef cattle. | PENG Quan-hui CHENG Long KANG Kun Tian Gang Mohammad Al-Mamun XUE Bai WANG Li-zhi ZOU Hua-wei Mathew Gitau Gicheha WANG Zhi-sheng | 2020 | Journal of Integrative Agriculture2020，19，3： | 9 |
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