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| 1 | Current status of diagnosis and treatment of bladder cancer in China-Analyses of Chinese Bladder Cancer Consortium database显示文摘Objective:To investigate current status of diagnosis and treatment of bladder cancer in China.Methods:A database was generated by Chinese Bladder Cancer Consortium(CBCC).From January 2007 to December 2012,14,260 cases from 44 CBCC centers were included.Data of diagnosis,treatment and pathology were collected.Results:The average age was 63.5 year-old and most patients were male(84.3%).The most common histologic types were urothelial carcinoma(91.4%),adenocarcinoma(1.8%),and squamous carcinoma(1.9%).According to 1973 and 2004 WHO grading system,42.0%,41.0%,and 17.0% of patients were grade 1,2,and 3,and 16.0%,48.7%,and 35.3% of patients were papillary urothelial neoplasms of low malignant potential,low,and high grade,respectively.Non-muscle invasive bladder cancer(NMIBC)and muscle invasive bladder cancer(MIBC)were 25.2% and 74.1%,respectively(0.8% not clear).Carcinoma in situ was only 2.4%.Most patients were diagnosed by white-light cystoscopy with biopsy(74.3%).Fluorescence and narrow band imaging cystoscopy had additional detection rate of 1.0% and 4.0%,respectively.Diagnostic transurethral resection(TUR)provided detection rate of 16.9%.Most NMIBCs were treated with TUR(89.2%).After initial TUR,2.6%accepted second TUR,and 45.7%,69.9%,and 58.7% accepted immediate,induced,and maintenance chemotherapy instillation,respectively.Most MIBCs were treated with radical cystectomy(RC,59.7%).Laparoscopic RCs were 35.1%,while open RC 63.4%.Extended and standard pelvic lymph node dissection were 7% and 66%,respectively.Three most common urinary diversions were orthotopic neobladder(44%),ileal conduit(31%),and ureterocutaneostomy(23%).Only 2.3% of patients accepted neo-adjuvant chemotherapy and only 18%of T3 and T4 patients accepted adjuvant chemotherapy.Conclusion:Disease characteristics are similar to international reports,while differences of diagnosis and treatment exist.This study can provide evidences for revisions of the guideline on bladder cancer in China. | Kaiwen Li Tianxin Lin 无 Wei Xue Xin Mu Enci Xu Xu Yang Fubao Chen Guangyong Li Lulin Ma Guoliang Wang Chaozhao Liang Haoqiang Shi Ming Li Mao Tang Xueyi Xue Yisong Lv Yaoliang Deng Chengyang Li Zhiwen Chen Xiaozhou Zhou Fengshuo Jin Xudong Liu Jinxin Wei Lei Shi Xin Gou Weiyang He Liqun Zhou Lin Cai Baiye Jin Guanghou Fu Xiangbo Kong Hongyan Sun Ye Tian Lang Feng Tiejun Pan Yiyi Wu Dongwen Wang Hailong Hao Benkang Shi Yaofeng Zhu Qiang Wei Ping Han Changli Wu Dawei Tian Zhangqun Ye Zheng Liu Zhiping Wang Junqiang Tian Lin Qi Minfeng Chen Wei Li Jinchun Qi Gongxian Wang Longlong Fu Zhaolin Sun Guangheng Luo Zhoujun Shen Zhaowei Zhu Jinchun Xing Zhun Wu Dong Wei Xin Chen Yanqun Na Hongfeng Guo Chunxi Wang Zhihua Lu Chuize Kong Yang Liu Jin Yang Jianyun Hu Xin Gao Jielin Li Changjun Yin Pu Li Shan Chen Zhen Du Jiongming Li Yongji Yan Xu Zhang Shuang Huang Fangjian Zhou Zhiling Zhang Yinghao Sun Shuxiong Zeng Song Cen Jiaquan Zhou Hanzhong Li Jin Wen Jian Huang | 2015 | Asian Journal of Urology2015，2，2： | 21 |
| 2 | Genotypic variations in the accumulation of Cd exhibited by different vegetables显示文摘It is an important approach to use the Cd-contaminated soils properly by growing low accumulator or excluder plants for Cd to produce safe foods. To find the suitable vegetable species for growing in Cd-contaminated soils, in the present study the variations in the Cd accumulation for twenty eight vegetable species and several cultivars of five common vegetables (cowpea, kidney pea, bitter gourd, cucumber and squash) were investigated in two soil Cd levels (1 and 2 mg/kg Cd). Experimental results showed that highly significant differences in Cd concentration were evident among 28 vegetables. For example, spinach Cd concentrations were 110-fold and 175-fold higher than that of sweet pea under the 1 and 2 mg/kg Cd exposures, respectively. For Cd accumulation, the order of vegetable species was: leafy vegetables > solanaceous vegetables > kale vegetables > root vegetables > allimus > melon vegetables > legumes. Distinctive differences were also identified when comparing different cultivars of the five common vegetables with an average range of 0.003–0.094 mg/kg Cd. Our results indicated that a large genotypic variation existed among vegetable species or cultivars when subjected to Cd exposure. Therefore, it is important and feasible to elect/breed vegetable species/cultivars with low accumulation of Cd, especially in mildly Cd-contaminated soils. | Junxing Yang Haitao Guo Yibing Ma Liqun Wang Dongpu Wei Luo Hua | 2010 | Journal of Environmental Sciences2010，22，8： | 16 |
| 3 | Loss of 5-hydroxymethylcytosine is linked to gene body hypermethylation in kidney cancer显示文摘5-methylcytosine (5mC ) 和它的氧化形式 5-hydroxymethylcytosine (5hmC ) 被建议了涉及 tumorigenesis。因为印射方法的广泛地使用的 5mC 的读出,定序的重亚硫酸盐(BS-seq ) ,是 5mC 的和, 5hmC 层次, 5mC/5hmC 模式和这二修正的关系仍然保持糟糕理解。由介绍真实 5mC (BS-seq 由帮助 Tet 的 BS-seq 改正了, TAB-seq ) 并且 5hmC (TAB-seq ) 在单个核苷酸的分辨率同时铺平,我们这里证明与匹配的正常纸巾相比没有在肾肿瘤的 5mC 的全球损失。相反地, 5hmC 全球性被失去在几乎所有肾肿瘤纸巾。在肿瘤纸巾的 5hmC 水平是为肾癌症的一个独立预示的标记,与与更短的全面幸存联系的 5hmC 的底层。而且,我们证明 5hmC 的损失与匹配的正常纸巾相比在肿瘤被连接到 hypermethylation,特别地在基因身体区域。惊人地,基因身体 hypermethylation 显著地与肿瘤相关的基因的 silencing 被联系。IDH1 的 Downregulation 作为在肾癌症位于 5hmC 损失下面的机制被识别。恢复 5hmC 层次稀释了肿瘤房间的侵略能力并且在一个异种皮移植模型压制了肿瘤生长。一起,我们的结果证明 5hmC 的损失在由改变 DNA methylation 模式的肾癌症是一个预示的标记和一个 oncogenic 事件。 | Ke Chen Jing Zhang Zhongqiang Guo Qin Ma Zhengzheng Xu Yuanyuan Zhou Ziying Xu Zhongwu Li Xiongjun- Ye Xuesong Li Bifeng Yuan Yuwen Ke Chuan He Liqun Zhou Jiang Liu WeiminCi | 2016 | Cell Research2016，26，1： | 10 |
| 4 | Mutation profile and its correlation with clinicopathology in Chinese hepatocellular carcinoma patients显示文摘Background:Hepatocellular carcinoma(HCC)is one of the most common causes of cancer worldwide.Although many studies have focused on oncogene characteristics,the genomic landscape of Chinese HCC patients has not been fully clarified.Methods:A total of 165 HCC patients,including 146 males and 19 females,were enrolled.The median age was 55 years(range,27-78 years).Corresponding clinical and pathological information was collected for further analysis.A total of 168 tumor tissues from these patients were selected for next-generation sequencing(NGS)-based 450 panel gene sequencing.Genomic alterations including single nucleotide variations(SNV),short and long insertions and deletions(InDels),copy number variations,and gene rearrangements were analyzed.Tumor mutational burden(TMB)was measured by an algorithm developed in-house.The top quartile of HCC was classified as TMB high.Results:A total of 1,004 genomic alterations were detected from 258 genes in 168 HCC tissues.TMB values were identified in 160 HCC specimens,with a median TMB of 5.4 Muts/Mb(range,0-28.4 Muts/Mb)and a 75%TMB of 7.7 Muts/Mb.The most commonly mutated genes were TP53,TERT,CTNNB1,AXIN1,RB1,TSC2,CCND1,ARID1A,and FGF19.SNV was the most common mutation type and C:G>T:A and guanine transformation were the most common SNVs.Compared to wild-type patients,the proportion of Edmondson grade III-IV and microvascular invasion was significantly higher in TP53 mutated patients(P<0.05).The proportion of tumors invading the hepatic capsule was significantly higher in TERT mutated patients(P<0.05).The proportion of Edmondson grade I-II,alpha fetoprotein(AFP)<25μmg/L,and those without a history of hepatitis B was significantly higher in CTNNB1 mutated patients(P<0.05).CTNNB1 mutations were associated with TMB high in HCC patients(P<0.05).Based on correlation analysis,the mutation of TP53 was independently correlated with microvascular invasion(P=0.002,OR=3.096)and Edmondson grade III-IV(P=0.008,OR=2.613).The mutation of TERT was independently correlated with tumor invasion of the liver capsule(P=0.001,OR=3.030),and the mutation of CTNNB1 was independently correlated with AFP(<25μmg/L)(P=0.009,OR=3.414).Conclusions:The most frequently mutated genes of HCC patients in China were TP53,TERT,and CTNNB1,which mainly lead to the occurrence and development of HCC by regulating the P53 pathway,Wnt pathway,and telomere repair pathway.There were more patients with microvascular invasion and Edmondson III-IV grade in TP53 mutated patients and more patients with hepatic capsule invasion in TERT mutated patients,while in CTNNB1 mutated patients,there were more patients with Edmondson I-II grade,AFP<25μmg/L,and a non-hepatitis B background.Also,the TMB values were significantly higher in CTNNB1 mutated patients than in wild type patients. | Shuo Wang Huasheng Shi Tao Liu Manjiang Li Sanshun Zhou Xuan Qiu Zusen Wang Weiyu Hu Weidong Guo Xiaoqian Chen Honglin Guo Xiaoliang Shi Junping Shi Yunjin Zang Jingyu Cao Liqun Wu | 2021 | Hepatobiliary Surgery and Nutrition2021，10，2： | 8 |
| 5 | Efficacy of sunitinib in patients with metastatic renal cell carcinoma: initial experience in two Chinese centers显示文摘 | He Zhisong Guo Gang Zhang Cuijian Li Xuesong Fu Weijun Jin Jie Zhang Xu Zhou Liqun | 2014 | Chinese Medical Journal2014，，8： | 5 |
| 6 | Effects of Rhizoma Acori Tatarinowii extracts on gamma-aminobutyric acid type A receptor alpha 1 subunit brain expression during development in a recurrent seizure rat model显示文摘Extracts from Rhizoma Acori Tatarinowii (Grassleaf Sweetflag Rhizome,Shichangpu) have been shown to improve learning and memory,reduce anxiety,allay excitement,and suppress seizures.Rhizoma Acori Tatarinowii extracts interact with γ-aminobutyric acid and activate the γ-aminobutyric acid type A receptor,although few studies have addressed the precise effects of γ-aminobutyric acid type A receptor α1 subunit.In the present study,γ-aminobutyric acid type A receptor α1 subunit protein expression in the cerebral cortex and hippocampus,and pathological scores of brain injury,were significantly greater following recurrent seizures,but significantly decreased following treatment with Rhizoma Acori Tatarinowii extracts.These results indicated that Rhizoma Acori Tatarinowii extracts down-regulated γ-aminobutyric acid type A receptor α1 subunit protein expression in the cerebral cortex and hippocampus and protected seizure-induced brain injury during development. | Liqun Liu Ding'an Mao Keqiang Chi Xingfang Li Tao Bo Jinming Guo Zhuwen Yi | 2011 | Neural Regeneration Research2011，6，6： | 4 |
| 7 | B7-DC (PD-L2) costimulation of CD4^(+) T-helper 1 response via RGMb显示文摘The role of B7-DC in T-cell responses remains controversial because both coinhibitory and costimulatory functions have been reported in various experimental systems in vitro and in vivo.In addition to interacting with the coinhibitory receptor PD-1,B7-DC has also been shown to bind repulsive guidance molecule b(RGMb).The functional consequences of the B7-DC/RGMb interaction,however,remain unclear.More than a decade ago,we reported that replacement of a murine B7-DC mutant lysine with serine(K113S)at positive 113 resulted in a loss of binding capacity to PD-1.Nevertheless,K113S remained costimulatory for T cells in vitro,implicating a dual functionality for B7-DC in T-cell responses.Here we show that recombinant K113S protein interacts with RGMb with a similar affinity to wild-type B7-DC.More importantly,K113S costimulates CD4^(+)T-cell responses via RGMb and promotes Th1 polarization.RGMb is expressed on the surface of naive mouse T cells,macrophages,neutrophils and dendritic cells.Finally,K113S/RGMb costimulation suppresses Th2-mediated asthma and ameliorates small airway inflammation and lung pathology in an experimental mouse model.Our findings indicate that RGMb is a costimulatory receptor for B7-DC.These findings from the K113S variant provide not only a possible explanation for the B7-DC-triggered contradictory effects on T-cell responses,but also a novel approach to investigate the B7-DC/PD-1/RGMb axis.Recombinant K113S or its derivatives could potentially be developed as an agonist for RGMb to costimulate the Th1 response without triggering PD-1-mediated T-cell inhibition. | Xinxin Nie Wenni Chen Ying Zhu Baozhu Huang Weiwei Yu Zhanshuai Wu Sizheng Guo Yiping Zhu Liqun Luo Shengdian Wang Lieping Chen | 2018 | Cellular & Molecular Immunology2018，15，10： | 3 |
| 8 | Maximum power point tracking of PV system under partial shading conditions through flower pollination algorithm显示文摘For maximum utilization of solar energy,photovoltaic(PV)power systems should be operated at the maximum power point(MPP)which can be achieved using maximum power point tracking(MPPT)methods.However,the occurrence of multi-peak on P-V curve of a PV array due to the changing environmental conditions such as being partially shaded increases the complexity of the tracking process.The global MPP cannot always be achieved by the conventional MPPT methods.Therefore a novel MPPT method for PV systems using flower pollination(FP)algorithm is proposed in this paper and the Levy flight is used to improve the convergence of FP algorithm.MPPT model of the PV system is established in MATLAB to verify the effectiveness of the proposed method,and the proposed method is compared with two well established MPPT methods.The simulation results indicate that the proposed MPPT method can quickly track the changes in external environment and effectively handle the partially shaded condition. | Liqun Shang Weiwei Zhu Pengwei Li Hangchen Guo | 2018 | Protection and Control of Modern Power Systems2018，3，1： | 1 |
| 9 | Effects of rape straw and red mud on extractability and bioavailability of cadmium in a calcareous soil显示文摘划算的土壤修正案屏蔽是重要的在 situ 补习技术发展因为镉(Cd ) 污染了土壤。在这研究,不同土壤修正案包括红泥,氧化铝工业的一个副产品,并且酸处理,由 nanoparticle milling , nano 和酸处理的红 对待nano 泥,沸石,玉米稻草,和强奸稻草,被评估在二个增加的层次使 Cd 不能调动( 2 和 5 mg Cd 敲漠?業牣?汥'虪箾K獹獩爠慥瑣牯?桷捩?湩楤慣整?牧慥?潰整瑮慩?潦?桴?牰捡楴慣?灡汰捩瑡潩?景琠敨挠牥浡捩?湩琠敨映敩摬漠?捡畴污椠摮獵牴慩? | Junxing YANG Liqun WANG Jumei LI Dongpu WEI Shibao CHEN Qingjun GUO Yibing MA | 2015 | Frontiers of Environmental Science & Engineering2015，9，3： | 1 |
| 10 | 显示文摘 | Liqun Yan Qinhua Song Qingxiang Guo | 2002 | Chinese Journal of Organic Chemistry2002，22，12： | 1 |
| 11 | 靶向肾癌中的E3连接酶接头蛋白SPOP的小分子抑制剂显示文摘近年来肾癌发病率上升幅度在恶性肿瘤中排名第一。临床治疗表明,肾癌对放疗和化疗均不敏感,以索拉非尼和舒尼替尼为代表的靶向抗肿瘤药物是晚期肾癌的一线治疗药物,但对转移性肾癌的疗效十分有限,并且容易产生耐药。因此,发现并确证治疗肾癌特异性药物作用新靶标是一项十分紧迫并意义重大的任务。 | Zhong-Qiang Guo Tong Zheng Baoen Chen Cheng Luo Sisheng Ouyang Shouzhe Gong Jiafei Li Liu-Liang Mao Fulin Lian Yong Yang Yue Huang Li Li Jing Lu Bidong Zhang Luming Zhou Hong Ding Zhiwei Gao Liqun Zhou Guoqiang Li Ran Zhou Ke Chen Jingqiu Liu Yi Wen Likun Gong Yuwen Ke Shang-Dong Yang Xiao-Bo Qiu Naixia Zhang Jin Ren Dafang Zhong 杨财广 刘江 蒋华良 | 2017 | 科学新闻2017，19，4： | 1 |
| 12 | Internal Steel Strain Measurement inside of Reinforced Concrete by FBGS显示文摘 | Zhang Hong Tang Liqun Ren Peng Guo Jiansheng Lao Xiaochun | 2003 | SPIE2003，5058，： | 1 |
| 13 | Comparison of Pb(II) Immobilized by Bone Char Meal and Phosphate Rock: Characterization and Kinetic Study显示文摘 | Shibao Chen Yibing Ma Li Chen Liqun Wang Haitao Guo | 2010 | Archives of Environmental Contamination and Toxicology2010，，1： | 1 |
| 14 | An improved MPPT control strategy based on incremental conductance algorithm显示文摘PV power production is highly dependent on environmental and weather conditions,such as solar irradiance and ambient temperature.Because of the single control condition and any change in the external environment,the first step response of the converter duty cycle of the traditional MPPT incremental conductance algorithm is not accurate,resulting in misjudgment.To improve the efficiency and economy of PV systems,an improved incremental conductance algorithm of MPPT control strategy is proposed.From the traditional incremental conductance algorithm,this algorithm is simple in structure and can discriminate the instantaneous increment of current,voltage and power when the external environment changes,and so can improve tracking efficiency.MATLAB simulations are carried out under rapidly changing solar radiation level,and the results of the improved and conventional incremental conductance algorithm are compared.The results show that the proposed algorithm can effectively identify the misjudgment and avoid its occurrence.It not only optimizes the system,but also improves the efficiency,response speed and tracking efficiency of the PV system,thus ensuring the stable operation of the power grid. | Liqun Shang Hangchen Guo Weiwei Zhu | 2020 | Protection and Control of Modern Power Systems2020，5，1： | 1 |
| 15 | Strategy evaluation and optimization with an artificial society toward a Pareto optimum显示文摘Strategy evaluation and optimization in response to troubling urban issues has become a challenging issue due to increasing social uncertainty,unreliable predictions,and poor decision-making.To address this problem,we propose a universal computational experiment framework with a fine-grained artificial society that is integrated with data-based models.The purpose of the framework is to evaluate the consequences of various combinations of strategies geared towards reaching a Pareto optimum with regards to efficacy versus costs.As an example,by modeling coronavirus disease 2019 mitigation,we show that Pareto frontier nations could achieve better economic growth and more effective epidemic control through the analysis of real-world data.Our work suggests that a nation’s intervention strategy could be optimized based on the measures adopted by Pareto frontier nations through large-scale computational experiments.Our solution has been validated for epidemic control,and it can be generalized to other urban issues as well. | Zhengqiu Zhu Bin Chen Hailiang Chen Sihang Qiu Changjun Fan Yong Zhao Runkang Guo Chuan Ai Zhong Liu Zhiming Zhao Liqun Fang Xin Lu | 2022 | The Innovation2022，3，5： | 1 |
| 16 | Molecular characterization and functional analysis of daf-8 in the pinewood nematode,Bursaphelenchus xylophilus显示文摘Bursaphelenchus xylophilus,causal agent of pine wilt disease,causes extensive damage worldwide.Strate-gies are needed to inhibit population growth or block the spread of the invasive nematode to control pine wilt dis-ease.The gene daf-8 plays crucial roles in larval develop-ment and dauer formation in Caenorhabditis elegans,but little is known about its orthologue in B.xylophilus.In the present molecular characterization and functional analysis of daf-8 in B.xylophilus(Bx-daf-8),RT-qPCR showed that the expression of Bx-daf-8 gradually increased during the embryonic stage,peaked in the second-stage juvenile(J2),then dramatically dropped in the J3,and remained at that low level for the rest of its life.Bx-daf-8-transgenic C.elegans was employed to mimic the spatiotemporal expression of Bx-daf-8,which was expressed close to the pharynx during early embryogenesis and weakly throughout the whole body during late embryogenesis.It was observed in head neurons and tail ganglions throughout all postembryonic stages.B.xylophilus embryos were severely abnormal,and hatching rate decreased sharply after Bx-daf-8 knockdown.daf-16-1 and da-f16-2,downstream genes in the IIS pathway,also dropped sharply after Bx-daf-8 knockdown.We propose that TGFβmay crosstalk with the IIS pathway upstream of Bx-daf-16 and that daf-8 may act as a master regulator of daf-16 in B.xylophilus.However,knockdown of Bx-daf-8 did not lead to constitutive developmental arrest at the dauer larval stage,indicating that dauer entry in B.xylophilus might be controlled by several genes and is more complicated than in C.elegans.Bx-daf-8 alone did not control the dauer entry in B.xylophilus,but it was indispensable for embryogen-esis,providing a potential target for suppressing population growth of B.xylophilus. | Jinghan Wang Huan Hong Rong Xie Jingjing Ji Kai Guo Liqun Bai Jia Tang Hongshi Yu Jianren Ye Jiafu Hu | 2022 | Journal of Forestry Research2022，33，2： | 1 |
| 17 | Dispersing Graphene in Hydroxypropyl Cellulose by Utilizing its LCST Behavior显示文摘 | Ruijuan Liao Yanda Lei Jingjing Wan Zhenghai Tang Baochun Guo Liqun Zhang | 2012 | Macromol Chem Phys2012，，13： | 1 |
| 18 | Sesamin im- proves endothelial dysfunction in renovascular hypertensive rats fed with a high-fat, high-sucrose diet 显示文摘 | KONG XIANG YANG JIERAN GUO LIQUN | 2009 | Eur J Pharmacol2009，620，13： | 1 |
| 19 | Treating CsPbI_(3) Perovskite with Pyrrolidinium Iodide to Improve the Performance of Perovskite Solar Cells显示文摘All-inorganic CsPbI_(3) perovskite has attracted wide attention due to its desirable optical bandgap(Eg:∼1.7 eV)as well as high chemical stability.Nevertheless,the photovoltaic performance of CsPbI_(3) perovskite solar cells(PSCs)was limited by severe nonradiative charge recombination due to high defect density at the grain boundary and surface of perovskitefilms.To address this issue,a pyrrolidinium iodide(PyI)molecule was introduced to modify the surface and grain boundary of CsPbI_(3) perovskitefilms to passivate defects,which improves the quality of CsPbI_(3) perovskitefilms as well as induces the generation of a quasi-2D Py_(2)CsPb_(2)I_(7) capping layer between per-ovskite layer and hole transport layer.Such quasi-2D Py_(2)CsPb_(2)I_(7) capping layer optimizes interface contact between CsPbI_(3) perovskite layer and hole transport layer and blocks the electron transfer from CsPbI_(3) perovskite photoactive layer to the hole transport layer.As a result,the performance of CsPbI_(3) PSCs is well improved to 17.87%for power conversion efficiency(PCE)with an ultra-high fill factor(FF)of 0.84.In addition,the PyI mole-cule modified CsPbI_(3) perovskite devices exhibit excellent stability,which remains its initial PCE almost unchanged after aging for 35 days under the dry air atmosphere(temperature:20℃–30℃,control relative humid-ity(RH):<10%). | Qixian Zhang Yi Guo Huicong Liu Weiping Li Liqun Zhu Haining Chen | 2023 | Journal of Renewable Materials2023，11，8： | 0 |
| 20 | Rapid Quantitative Determination of Isoprene Monomer in Living Taraxacum kok-saghyz by Ultra-High Performance Liquid Chromatography Tandem Mass Spectrometry显示文摘Taraxacum kok-saghyz(TKS)is rich in natural rubber(NR),a natural organic macromolecular compound composed of cis-1,4-polyisoprene,and may become the second NR-bearing plant for biochemical engineering development.In this paper,a rapid and quantitative ultra-high performance liquid chromatography tandem mass spectrometry(UHPLCMS/MS)method was established for determination of macromolecular biosynthesis substrate(dimethylallyl pyrophosphate,DMAPP)and initiator(farnesyl pyrophosphate,FPP)contained in TKS.A Kromasil C18 chromatographic column was used for separation,and the multi-reaction monitoring mode(MRM)of triple quadrupole mass spectrometry was used for detection.Quantification was performed by external calibration method.The results showed that the limit of detection(LOD)and the limit of quantitation(LOQ)of DMAPP were 2.42μg/L and 7.26μg/L,respectively,and the LOQ and the LOD of FPP were 1.02μg/L and 3.05μg/L,respectively.At a concentration of 1—1000μg/L,both analytes had good determination coefficients(>0.999)of calibration curve.The recoveries of DMAPP and FPP were between 99.0%and 117.1%.In real samples detection,the contents of DMAPP and FPP in TKS samples were between 23.32—82.77μg/L and 12.03—85.67μg/L,respectively.Thus,this approach is a reliable method to quantify DMAPP and FPP in TKS. | Xiang Tong Guo Tianyang Zhang Xi Chen Yunhan Dong Yiyang Zhang Jichuan Ma Qiang Zhang Liqun | 2020 | China Petroleum Processing & Petrochemical Technology2020，22，2： | 0 |
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