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64篇 您的检索式:作者名="Lingfeng Chen"
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1Inflammatory response and neuronal necrosis in rats with cerebral ischemia显示文摘In the middle cerebral artery occlusion model of ischemic injury, inflammation primarily occurs in the infarct and peripheral zones. In the ischemic zone, neurons undergo necrosis and apoptosis, and a large number of reactive microglia are present. In the present study, we investigated the pathological changes in a rat model of middle cerebral artery occlusion. Neuronal necrosis appeared 12 hours after middle cerebral artery occlusion, and the peak of neuronal apoptosis appeared 4 to 6 days after middle cerebral artery occlusion. Inflammatory cytokines and microglia play a role in damage and repair after middle cerebral artery occlusion. Serum intercellular cell adhesion molecule-1 levels were positively correlated with the permeability of the blood-brain barrier. These findings indicate that intercellular cell adhesion molecule-1 may be involved in blood-brain barrier injury, microglial activation, and neuronal apoptosis. Inhibiting blood-brain barrier leakage may alleviate neuronal injury following ischemia.Lingfeng Wu Kunnan Zhang Guozhu Hu Haiyu Yang Chen Xie Xiaomu Wu 2014Neural Regeneration Research2014,9,19:19
2Determination of rice panicle numbers during heading by multi-angle imaging显示文摘Plant phenomics has the potential to accelerate progress in understanding gene functions and environmental responses. Progress has been made in automating high-throughput plant phenotyping. However, few studies have investigated automated rice panicle counting. This paper describes a novel method for automatically and nonintrusively determining rice panicle numbers during the full heading stage by analyzing color images of rice plants taken from multiple angles. Pot-grown rice plants were transferred via an industrial conveyer to an imaging chamber. Color images from different angles were automatically acquired as a turntable rotated the plant. The images were then analyzed and the panicle number of each plant was determined. The image analysis pipeline consisted of extracting the i2 plane from the original color image, segmenting the image, discriminating the panicles from the rest of the plant using an artificial neural network, and calculating the panicle number in the current image. The panicle number of the plant was taken as the maximum of the panicle numbers extracted from all 12 multi-angle images. A total of 105 rice plants during the full heading stage were examined to test the performance of the method. The mean absolute error of the manual and automatic count was 0.5, with 95.3% of the plants yielding absolute errors within ± 1. The method will be useful for evaluating rice panicles and will serve as an important supplementary method for high-throughput rice phenotyping.Lingfeng Duan Chenglong Huang Guoxing Chen Lizhong Xiong Qian Liu Wanneng Yang 2015The Crop Journal2015,3,3:18
3PlantcircBase: A Database for Plant Circular RNAs显示文摘Qinjie Chu Xingchen Zhang Xintian Zhu Chen Liu Lingfeng Mao Chuyu Ye Qian-Hao Zhu Longjiang Fan 2017Molecular Plant2017,10,8:15
4An overview of the configuration and manipulation of soft robotics for on-orbit servicing显示文摘Soft robots refer to robots that are softer and more flexible when compared with conventional rigidbodied robots. Soft robots are adapted to unstructured environments due to their flexibility, deformability and energy-absorbing properties. Thus, they have tremendous application prospects in on-orbit servicing(OOS).This study discusses the configuration and manipulation of soft robotics. Usually, learning from living beings is used to develop the configurations of most soft robots. In this study, typical soft robots are introduced based on what they mimic. The discussion of manipulation is divided into two parts, namely actuation and control.The study also involves describing and comparing several types of actuations. Studies on the control of soft robots are also reviewed. In this study, potential application of soft robotics for on-orbit servicing is analyzed.A hybrid configuration and manipulation of space soft robots for future research are proposed based on the current development of soft robotics, and some challenges are discussed.Zhongliang JING Lingfeng QIAO Han PAN Yongsheng YANG Wujun CHEN 2017Science China(Information Sciences)2017,60,5:8
5Transcriptome analysis and metabolic profiling reveal the key role of carotenoids in the petal coloration of Liriodendron tulipifera显示文摘Liriodendron tulipifera,also known as tuliptree,is a popular ornamental horticultural plant with extraordinary tulipshaped flowers characterized by an orange band near their base.The mechanisms underlying petal band-specific pigmentation during L.tulipifera flower development are unclear.Here,we combined nontargeted and targeted metabolomics and transcriptomics to identify a pathway cascade leading to carotenoid biosynthesis that is specifically activated in the petal band.The comparative analysis of carotenoid metabolites between L.tulipifera and Liriodendron hybrids indicates thatγ-carotene,a rare carotene in plants,is the most likely orange pigment responsible for the coloration of the petal band.Phenotypic and transcriptomic analyses of developing petals reveal that the band area is first predefined by the loss of green color.Later,the band is maintained by locally activating and repressing carotenoid and chlorophyll biosynthesis genes,respectively.Two rate-limiting genes of carotene biosynthesis,carotenoid isomerase(CRTISO)and epsilon lycopene cyclase(ε-LCY),encode the core enzymes responsible for petal band-specific orange pigmentation in L.tulipifera.In particular,a putative additionalε-LCY copy specific to L.tulipifera may contribute to the distinct petal coloration pattern,compared with L.chinense.Taken together,our work provides a first glimpse of the metabolome and transcriptome dynamics in tuliptree flower coloration and provides a valuable resource for flower breeding or metabolic engineering as well as for understanding flower evolution in an early woody angiosperm.Zhaodong Hao Siqin Liu Lingfeng Hu Jisen Shi Jinhui Chen 2020Horticulture Research2020,7,1:7
6A deep learning-integrated micro-CT image analysis pipeline for quantifying rice lodging resistance-related traits显示文摘Lodging is a common problemin rice,reducing its yield andmechanical harvesting efficiency.Rice architecture is a key aspect of its domestication and a major factor that limits its high productivity.The ideal rice culm structure,includingmajor_axis_culm,minor axis_culm,andwall thickness_culm,is critical for improving lodging resistance.However,the traditionalmethod ofmeasuring rice culms is destructive,time consuming,and labor intensive.In this study,we used a high-throughput micro-CT-RGB imaging system and deep learning(SegNet)todevelopa high-throughputmicro-CTimageanalysis pipelinethatcanextract 24 riceculmmorphological traits and lodging resistance-related traits.When manual and automatic measurements were compared at themature stage,the mean absolute percentage errors for major_axis_culm,minor_axis_culm,andwall_thickness_culmin 104 indica rice accessionswere 6.03%,5.60%,and 9.85%,respectively,and the R^(2) valueswere 0.799,0.818,and 0.623.We also builtmodels of bending stress using culmtraits at the mature and tillering stages,and the R^(2) values were 0.722 and 0.544,respectively.The modeling results indicated that this method can quantify lodging resistance nondestructively,even at an early growth stage.In addition,we also evaluated the relationships of bending stress toshoot dryweight,culm density,and drought-related traits and found that plants with greater resistance to bending stress had slightly higher biomass,culm density,and culm area but poorer drought resistance.In conclusion,we developed a deep learning-integrated micro-CT image analysis pipeline to accurately quantify the phenotypic traits of rice culms in4.6 min per plant;this pipeline will assist in future high-throughput screening of large rice populations for lodging resistance.Di Wu Dan Wu Hui Feng Lingfeng Duan Guoxing Dai Xiao Liu Kang Wang Peng Yang Guoxing Chen Alan P.Gay John H.Doonan Zhiyou Niu Lizhong Xiong Wanneng Yang 2021Plant Communications2021,2,2:7
760 Years of Development of the Journal of Integrative Plant Biology显示文摘In celebration of JIPB's 60 th anniversary,this paper summarizes and reviews the development process of the journal.To start,we offer our heartfelt thanks to JIPB's pioneer Editors-in-Chief who helped get the journal off the ground and make it successful.Academic achievement is the soul of academic journals,and this paper summarizes JIPB's course of academic development by analyzing it in four stages:the first two stages are mostly qualitative analyses,and the latter two stages are dedicated to quantitative analyses.Most-cited papers were statistically analyzed.Improvements in editing,publication,distribution and online accessibility-which are detailed in this paper-contribute to JIPB's sustainable development.In addition,JIPB's evaluation index and awards are provided with accompanying pictures.At the end of the paper,JIPB's milestones are listed chronologically.We believe that JIPB's development,from a national journal to an international one,parallels the development of the Chinese plant sciences.Jinzhong Cui Ping He Fenghong Liu Jingjing Tan Lingfeng Chen Joshua Fenn 2012Journal of Integrative Plant Biology2012,54,10:5
8Flexible, transparent nanocellulose paper-based perovskite solar cells显示文摘Recently perovskite solar cells(PSCs),as photoelectric conversion devices,exhibit excellent power conversion efficiency(PCE)and low-processing cost,and have become one of the most promising devices to replace conventional silicon-based solar cells and address current pressing energy issues.Among them,the flexible PSCs are especially more widely applicable and may propel the rapid advancements of wearable electronics,causing a significant paradigm shift in consumer electronics.Current flexible PSCs use non-biodegradable petroleum-based polymer substrates,discarding of which will aggravate“white pollution”.Therefore,development of green,biodegradable and low-cost flexible substrates will provide a great alternative to flexible PSCs.Here we have developed transparent nanocellulose paper(NCP)with coating of acrylic resin as substrates to fabricate flexible PSCs,which are biodegradable and easily disposable.The PCE of these NCP-based PSCs reached 4.25%,while the power per weight(the ratio of power to device weight)was as high as 0.56 W g^(–1).The flexible PSCs also showed good stability,retaining>80%of original efficiency after 50 times of bending.The NCP-based substrates can also be applied to other electronic systems,which may prosper next-generation green flexible electronics.Lei Gao Lingfeng Chao Meihui Hou Jin Liang Yonghua Chen Hai-Dong Yu Wei Huang 2019npj Flexible Electronics2019,3,1:5
9The Genomes of the Allohexaploid Echinochloa crus-galli and Its Progenitors Provide Insights into Polyploidization-Driven Adaptation显示文摘The hexaploid species Echinochloa crus-galli is one of the most detrimental weeds in crop fields,especially in rice paddies.Its evolutionary history is similar to that of bread wheat,arising through polyploidization after hybridization between a tetraploid and a diploid species.In this study,we generated and analyzed high-quality genome sequences of diploid(E.haploclada),tetraploid(E.oryzicola),and hexaploid(E.crus-galli)Echinochloa species.Gene family analysis showed a significant loss of disease-resistance genes such as those encoding NB-ARC domain-containing proteins during Echinochloa polyploidization,contrary to their significant expansionduring wheat polyploidization,suggesting that natural selection might favor reduced investment in resistance in this weed to maximize its growth and reproduction.In contrast to the asymmetric patterns of genome evolution observed in wheat and other crops,no significant differences in selection pressure were detected between the subgenomes in E.oryzicola and E.crus-galli.In addition,distinctive differences in subgenome transcriptome dynamics during hexaploidization were observed between E.crus-galli and bread wheat.Collectively,our study documents genomic mechanisms underlying the adaptation of a major agricultural weed during polyploidization.The genomic and transcriptomic resources of three Echinochloa species and new insights into the polyploidization-driven adaptive evolution would be useful for future breeding cereal crops.Chu-Yu Ye Dongya Wu Lingfeng Mao Lei Jia Jie Qiu Sangting Lao Meihong Chen Bowen Jiang Wei Tang Qiong Peng Lang Pan Lifeng Wang Xiaoxiao Feng Longbiao Guo Chulong Zhang Elizabeth A.Kellogg Kenneth M.Olsen Lianyang Bai Longjiang Fan 2020Molecular Plant2020,13,9:4
10Roles of MicroRNAs in the Caenorhabditis elegans Nervous System显示文摘The first microRNA was discovered in Caenorhabditis elegans in 1993,and since then,thousands of microRNAs have been identified from almost all eukaryotic organisms examined.MicroRNAs function in many biological events such as cell fate determination,metabolism,apoptosis,and carcinogenesis.So far,more than 250 microRNAs have been identified in C.elegans;however,functions for most of these microRNAs are still unknown.A small number of C.elegans microRNAs are associated with known physiological roles such as developmental timing,cell differentiation,stress response,and longevity.In this review,we summarize known roles of microRNAs in neuronal differentiation and function of C.elegans,and discuss interesting perspectives for future studies.Lingfeng Meng Liang Chen Zhaoyong Li Zheng-Xing Wu Ge Shan 2013Journal of Genetics and Genomics2013,40,9:4
11Temporal and spatial comparisons of angiosperm diversity between eastern Asia and North America显示文摘Comparative approaches that examine floras with similar environments, but different diversity patterns, provide good opportunities to elucidate how uneven distributions of biodiversity were assembled. Eastern Asia(EA) and North America north of Mexico(NA;see definitions of EA and NA in Fig. S1 A) share similar geographical environments with comparable latitude, land area and climate.Haihua Hu Jianfei Ye Bing Liu Lingfeng Mao Stephen A.Smith Russell L.Barrett Pamela S.Soltis Douglas E.Soltis Zhiduan Chen Limin Lu 2022National Science Review2022,9,6:3
12Altitudinal patterns of maximum plant height on the Tibetan Plateau显示文摘Aims Several studies have shown that plant height changes along environmen-tal gradients.However,altitudinal patterns of plant height across species are still unclear,especially in regions sensitive to climate change.As canopy height decreases dramatically near the tree line in alpine areas,we hypothesize that plant height across all species also decreases with increasing altitude,and distinct thresholds exist along this gradient.Methods Using a large dataset of maximum plant height and elevation range(400 to 6000 m a.s.l.)of 4295 angiosperms from the regional flora of the Tibetan Plateau,we regressed plant height for every 100 m belt against elevation to explore the relationships.To identify the approximate boundaries where dramatic changes in plant height occurs for herbaceous plants,shrubs,trees,woody plants and all angiosperms,we used piecewise linear regression.Phylogenetically independent contrast was used to test the potential evolutionary influences on altitudinal patterns at the family level.Important Findings Results showed that for herbaceous plants,shrubs,trees,woody plants and all angiosperms,plant height decreases significantly as altitude increases.In addition,we found that altitude,a proxy for many environmental factors,had obvious thresholds(breakpoints)dictating patterns of plant height.The results of phylogenetically independent contrast also emphasized the importance of evolution-ary history in determining the altitudinal patterns of plant height for some growth forms.Our results highlight the relative intense filter-ing effect of environmental factors in shaping patterns of functional traits and how this could vary for different ranges of environmental variables.Lingfeng Mao Shengbin Chen Jinlong Zhang Guangsheng Zhou 2018Journal of Plant Ecology2018,11,1:3
13The role of γ-aminobutyric acid in aluminum stress tolerance in a woody plant, Liriodendron chinense×tulipifera显示文摘The aluminum(Al)cation Al^(3+)in acidic soil shows severe rhizotoxicity that inhibits plant growth and development.Most woody plants adapted to acidic soils have evolved specific strategies against Al^(3+)toxicity,but the underlying mechanism remains elusive.The four-carbon amino acid gamma-aminobutyric acid(GABA)has been well studied in mammals as an inhibitory neurotransmitter;GABA also controls many physiological responses during environmental or biotic stress.The woody plant hybrid Liriodendron(L.chinense×tulipifera)is widely cultivated in China as a horticultural tree and provides high-quality timber;studying its adaptation to high Al stress is important for harnessing its ecological and economic potential.Here,we performed quantitative iTRAQ(isobaric tags for relative and absolute quantification)to study how protein expression is altered in hybrid Liriodendron leaves subjected to Al stress.Hybrid Liriodendron shows differential accumulation of several proteins related to cell wall biosynthesis,sugar and proline metabolism,antioxidant activity,cell autophagy,protein ubiquitination degradation,and anion transport in response to Al damage.We observed that Al stress upregulated glutamate decarboxylase(GAD)and its activity,leading to increased GABA biosynthesis.Additional GABA synergistically increased Al-induced antioxidant enzyme activity to efficiently scavenge ROS,enhanced proline biosynthesis,and upregulated the expression of MATE1/2,which subsequently promoted the efflux of citrate for chelation of Al^(3+).We also showed similar effects of GABA on enhanced Al^(3+)tolerance in Arabidopsis.Thus,our findings suggest a function of GABA signaling in enhancing hybrid Liriodendron tolerance to Al stress through promoting organic acid transport and sustaining the cellular redox and osmotic balance.Pengkai Wang Yini Dong Liming Zhu Zhaodong Hao LingFeng Hu Xiangyang Hu Guibin Wang Tielong Cheng Jisen Shi Jinhui Chen 2021Horticulture Research2021,8,1:3
14Recent progress on low dimensional perovskite solar cells显示文摘Low dimensional perovskites have recently attracted much attention due to their vertical growth of crystalline orientation, excellent film morphology, and long-term humidity, light, and heat stability. However, low dimensional perovskites suffer from low power conversion efficiency(PCE) with respect to their three dimensional analogues. Therefore, it is imperative to find excellent low-dimensional perovskite materials for improving the PCE. Previous work has demonstrated that bulkier organic molecules,e.g., C_6 H_5(CH_2)_2 NH_3^+(PEA^+), CH_3(CH_2)_3 NH_3^+(n-BA+, iso-BA+), C_2H_4NH_3^+, and polyethylenimine cations(PEI+), play an important role in the formation of low-dimensional perovskites. In this review, we review the recent development of low dimensional perovskites for solar cells application in terms of film preparation, photophysics, and stability of perovskites, as well as the related device structure and physics. We have also discussed the future development of low-dimensional perovskites from materials design, fabrication processes, and device structure.Lingfeng Chao Ze Wang Yingdong Xia Yonghua Chen Wei Huang 2018Journal of Energy Chemistry2018,27,4:3
15The electromechanical features of LiNbO_(3) crystal for potential high temperature piezoelectric applications显示文摘Lithium niobate(LiNbO_(3),LN)crystal is a multi-functional material with favorable piezoelectric,nonlinear optical and electro-optic properties.In this study,the electromechanical properties of the radial extensional(RE)and the thickness extensional(TE)modes of the congruent LN are studied and the temperature dependent behaviors are revealed.The RE mode electromechanical coupling factors(kp)for the Y-and Z-oriented discs are calculated and found to be 3.8%and 24.7%,respectively,which are nearly the same as the experimental results of 3.8%and 25.2%,respectively.The maximum RE and thickness shear(TS)modes electromechanical coupling factors are obtained to be 47.6%and 68.5%for the Yx/25and Yx/167crystal cuts,respectively.The LN crystal possesses good temperature stability of the electromechanical coupling factors(RE and TE modes)from 20℃ to 500℃,where the variations of kp and kt for the Y-oriented discs are<8.0%and<1.8%,respectively.Feifei Chen Lingfeng Kong Wei Song Chao Jiang Shiwei Tian Fapeng Yu Lifeng Qin Xian Zhao 2019Journal of Materiomics2019,5,1:3
16Environmental RNA interference in animals显示文摘Animals interact with their environments all the time,and must react to all kinds of factors present in the environments.Environmental RNA interference refers to the phenomenon that exogenous RNA molecules can enter cells of certain animal species and interfere with gene expression of these animals.These exogenous RNAs may be molecules in animal food,or may be present in the living environment from other sources.Molecular pathways for the cell entry of environmental RNAs and further for their functionality in gene interference have started to be revealed in the animal model Caenorhabditis elegans.Here we highlight some known examples of environmental RNA interference(RNAi)in animals and introduce the molecular mechanisms underneath.MENG LingFeng CHEN Liang LI ZhaoYong WU ZhengXing SHAN Ge 2013Chinese Science Bulletin2013,58,35:3
17Berlin Green Framework‑Based Gas Sensor for Room‑Temperature and High‑Selectivity Detection of Ammonia显示文摘Ammonia detection possesses great potential in atmosphere environmental protection,agriculture,industry,and rapid medical diagnosis.However,it still remains a great challenge to balance the sensitivity,selectivity,working temperature,and response/recovery speed.In this work,Berlin green(BG)framework is demonstrated as a highly promising sensing material for ammonia detection by both density functional theory simulation and experimental gas sensing investigation.Vacancy in BG framework offers abundant active sites for ammonia absorption,and the absorbed ammonia transfers sufficient electron to BG,arousing remarkable enhancement of resistance.Pristine BG framework shows remarkable response to ammonia at 50–110°C with the highest response at 80°C,which is jointly influenced by ammonia’s absorption onto BG surface and insertion into BG lattice.The sensing performance of BG can hardly be achieved at room temperature due to its high resistance.Introduction of conductive Ti3CN MXene overcomes the high resistance of pure BG framework,and the simply prepared BG/Ti3CN mixture shows high selectivity to ammonia at room temperature with satisfying response/recovery speed.Tingqiang Yang Lingfeng Gao Wenxuan Wang Jianlong Kang Guanghui Zhao Delong Li Wen Chen Han Zhang 2021Nano-Micro Letters2021,13,4:2
18High-Performance Photo-Modulated Thin-Film Transistor Based on Quantum dots/Reduced Graphene Oxide Fragment-Decorated ZnO Nanowires显示文摘In this paper, a photo-modulated transistor based on the thin-film transistor structure was fabricated on the flexible substrate by spin-coating and magnetron sputtering. A novel hybrid material that composed of Cd Se quantum dots and reduced graphene oxide(RGO) fragment-decorated ZnO nanowires was synthesized to overcome the narrow optical sensitive waveband and enhance the photo-responsivity. Due to the enrichment of the interface and heterostructure by RGO fragments being utilized, the photo-responsivity of the transistor was improved to 2000 AW^(-1) and the photo-sensitive wavelength was extended from ultraviolet to visible. In addition, a positive back-gate voltage was employed to reduce the Schottky barrier width of RGO fragments and ZnO nanowires. As a result, the amount of carriers was increased by 10 folds via the modulation of back-gate voltage. With these inherent properties, such as integrated circuit capability and wide optical sensitive waveband, the transistor will manifest great potential in the future applications in photodetectors.Zhi Tao Yi-an Huang Xiang Liu Jing Chen Wei Lei Xiaofeng Wang Lingfeng Pan Jiangyong Pan Qianqian Huang Zichen Zhang 2016Nano-Micro Letters2016,8,3:2
19Costunolide covalently targets NACHT domain of NLRP3 to inhibit inflammasome activation and alleviate NLRP3-driven inflammatory diseases显示文摘The NLRP3 inflammasome’s core and most specific protein,NLRP3,has a variety of functions in inflammation-driven diseases.Costunolide(COS)is the major active ingredient of the traditional Chinese medicinal herb Saussurea lappa and has anti-inflammatory activity,but the principal mechanism and molecular target of COS remain unclear.Here,we show that COS covalently binds to cysteine 598 in NACHT domain of NLRP3,altering the ATPase activity and assembly of NLRP3 inflammasome.We declare COS’s great anti-inflammasome efficacy in macrophages and disease models of gouty arthritis and ulcerative colitis via inhibiting NLRP3 inflammasome activation.We also reveal that theα-methylene-γ-butyrolactone motif in sesquiterpene lactone is the certain active group in inhibiting NLRP3 activation.Taken together,NLRP3 is identified as a direct target of COS for its anti-inflammasome activity.COS,especially theα-methylene-γ-butyrolactone motif in COS structure,might be used to design and produce novel NLRP3 inhibitors as a lead compound.Haowen Xu Jiahao Chen Pan Chen Weifeng Li Jingjing Shao Shanshan Hong Yi Wang Lingfeng Chen Wu Luo Guang Liang 2023Acta Pharmaceutica Sinica B2023,13,2:2
20Origin of High Efficiency and Long-Term Stability in Ionic Liquid Perovskite Photovoltaic显示文摘Environment-friendly protic amine carboxylic acid ionic liquids(ILs)as solvents is a significant breakthrough with respect to traditional highly coordinating and toxic solvents in achieving efficient and stable perovskite solar cells(PSCs)with a simple one-step air processing and without an antisolvent treatment approach.However,it remains mysterious for the improved efficiency and stability of PSCs without any passivation strategy.Here,we unambiguously demonstrate that the three functions of solvents,additive,and passivation are present for protic amine carboxylic acid ILs.We found that the ILs have the capability to dissolve a series of perovskite precursors,induce oriented crystallization,and chemically passivate the grain boundaries.This is attributed to the unique molecular structure of ILs with carbonyl and amine groups,allowing for strong interaction with perovskite precursors by forming C=O…Pb chelate bonds and N-H…I hydrogen bonds in both solution and film.This finding is generic in nature with extension to a wide range of IL-based perovskite optoelectronics.Lingfeng Chao Tingting Niu Hao Gu Yingguo Yang Qi Wei Yingdong Xia Wei Hui Shouwei Zuo Zhaohua Zhu Chengjie Pei Xiaodong Li Jing Zhang Junfeng Fang Guichuan Xing Hai Li Xiao Huang Xingyu Gao Chenxin Ran Lin Song Li Fu Yonghua Chen Wei Huang 2020Research2020,,1:2
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