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| 1 | Direct reprogramming of Sertoli cells into multipotent neural stem cells by defined factors显示文摘Multipotent 神经干细胞为房间治疗保持大诺言。到导致的 pluripotent 干细胞以及成熟神经原的成纤维细胞的 reprogramming 建议可能性没有首先建立 pluripotency,把一个严重地区分的体的房间变换成一个 multipotent 状态。这里,我们证明房间从中层导出的那 Sertoli 能直接被变换成拥有神经干细胞性质的一个 multipotent 状态。导致的神经干细胞(iNSCs ) 表示多重 NSC 特定的标记,展出类似于正常 NSC 的全球基因表示侧面,并且能够自强并且区分功能的神经原进神经胶质并且 electrophysiologically。为酷氨酸 hydroxylase (TH ) 积极的导出 iNSC 的神经原污点, γ -aminobutyric 酸,和胆碱 acetyltransferase。另外, iNSCs 能熬过并且产生跟随移植进有牙齿的回转的触处。iNSCs 的产生可以为疾病建模和再生药有重要含意。 | Chao Sheng Qinyuan Zheng Jianyu Wu Zhen Xu Libin Wang Wei Li Haijiang Zhang Xiao-Yang Zhao Lei Liu Ziwei Wang Changlong Guo Hua-Jun Wu Zhonghua Liu Liu Wang Shigang He Xiu-Jie Wang Zhiguo Chen Qi Zhou | 2012 | Cell Research2012，22，1： | 31 |
| 2 | Research on key techniques of expendable conductivity temperature depth measuring system显示文摘This paper analysis the developing of expendable conductivity temperature depth measuring system(XCTD)and introduce its principle of measuring about temperature,salinity and depth of ocean.Some key techniques are put forward.According to the real needs of XCTD,conductivity sensor with high sensitivity is designed by principle of electromagnetic induce,the ocean conductivity from induced electromotive force has been calculated.Adding temperature correction circuit would help to reduce error of conductivity measurement because of sharply changing temperature.Advanced temperature measuring circuit of high precision and the constant current source is used to weaken effect of self-heating of resistance and fluctuation of the source.On respect of remote data transmission,LVDS is a good choice for the purpose of guarantee the quality of data transmitted and the transmission distance is reaching to thousand meters in the seawater.Modular programming method is also brought into this research aimed at improve the stability,reliability and maintainability of the whole measuring system.In February,2015,the trials in South China Sea demonstrate that the developed XCTD realize effective measurement at a speed of 6 knots and detection depth at 800 m.The consistency coefficient of the acquired data is greater than 0.99 and the success rate of probe launching is above 90%. | CHEN Guangyuan DU Libin HE Haijing LEI Zhuo ZHANG Qisheng WU Chengxuan | 2015 | Instrumentation2015，2，2： | 5 |
| 3 | Volatile components,total phenolic compounds,and antioxidant capacities of worm-infected Gomphidius rutilus显示文摘This study evaluated the effects of worm infection on the volatile components,total phenolic compounds,and antioxidant capacities of Gomphidius rutilus.G.rutilus without worms(GW),G.rutilus infected by a small amount of worms(GS;infected area<50%),and G.rutilus infected by a large amount of worms(GL;infected area>50%)were investigated.The volatile components of G.rutilus were analyzed by simultaneous distillation–extraction(SDE)and headspace solid-phase microextraction(HS-SPME)using gas chromatography–mass spectrometry(GC–MS).A total of 17 and 19 types of volatile compounds were detected,including ketones,alcohols,benzene,alkenes,aldehydes,esters,acids,and alkanes.Alcohols comprised the most abundant compound in GL,GS,and GW.The relative content of 1-octen-3-ol was the highest in all mushrooms.The concentration of eight-carbon(C8)compounds relative to the total volatile compounds varied widely,ranging from 40%(GW)to 64.34%(GS)and 84.42%(GS)and to 91.59%(GL),respectively,among the three samples.The antioxidant capability and the total phenolic contents of G.rutilus were evaluated in this study.The highest total phenolic content(TPC)of 192.23 mg GAE/g was found in GL,which differed significantly(P<0.05)from the latter two samples,whereas the lowest value of 156.11 mg GAE/g was found in GW.ABTS radical cation scavenging activity,FRAP ferric reducing antioxidant capacity(FRAP)radical scavenging activity,and oxygen radical absorbance capacity(ORAC)were investigated to screen the antioxidant properties of extracts.The contents of total phenolic compounds and their antioxidant capacities in vitro showed significant correlations(P<0.01).Among the three types of samples,the phenolic compounds of GL exhibited the highest antioxidant capacity,showing the values of 0.089 mM TE/g for ABTS,0.949 mM Fe^2+E/g for FRAP,and 1.952 M TE/g for ORAC.However,regarding the total antioxidant capacity,GS exhibited the highest antioxidant capacity,showing the values of 0.002648 mM TE/g for ABTS,0.004437 mM Fe^2+E/g for FRAP,and 0.256μM TE/g for ORAC.In conclusion,HS-SPME was more suitable for the extraction of volatile aroma components from G.rutilus.GL had the most abundant aroma components.GL had the highest TPC and antioxidant capacity compared with those of GS and GW,whereas GS showed the opposite results.Interestingly,GS was found to have the highest total antioxidant capacity in vitro.Based on these measured indicators,worm infection had no negative effect on the quality of G.rutilus.Therefore,worm-infected G.rutilus can also be consumed by humans. | Libin Sun Wei He Guang Xin Pengju Cai Yin Zhang Zhiyong Zhang Yunyun Wei Bingxin Sun Xiaowen Wen | 2018 | Food Science and Human Wellness2018，7，2： | 2 |
| 4 | H7N9 Influenza Viruses Are Transmissible in Ferrets by Respiratory Droplet显示文摘 | Qianyi Zhang Jianzhong Shi Guohua Deng Jing Guo Xianying Zeng Xijun He Huihui Kong Chunyang Gu Xuyong Li Jinxiong Liu Guojun Wang Yan Chen Liling Liu Libin Liang Yuanyuan Li Jun Fan Jinliang Wang Wenhui Li Lizheng Guan Qimeng Li Huanliang Yang Pucheng Che | 2013 | Science2013，，6144： | 1 |
| 5 | Simultaneous adsorption of As(Ⅲ) and Pb(Ⅱ) by the iron-sulfur codoped biochar composite:Competitive and synergistic effects显示文摘Simultaneous elimination of As(Ⅲ) and Pb(Ⅱ) from wastewater is still a great challenge.In this work,an iron-sulfur codoped biochar (Fe/S-BC) was successfully fabricated in a simplified way and was applied to the remediate the co-pollution of As(Ⅲ) and Pb(Ⅱ).The positive enthalpy indicated that the adsorption in As-Pb co-pollution was an endothermic reaction.The mechanism of As(Ⅲ) removal could be illustrated by surface complexation,oxidation and precipitation.In addition to precipitation and complexation,the elimination mechanism of Pb(Ⅱ) also contained ion exchange and electrostatic interactions.Competitive and synergistic effects existed simultaneously in the co-contamination system.The suppression of As(Ⅲ) was ascribed to competitive complexation of the two metals on Fe/S-BC,while the synergy of Pb(Ⅱ) was attributed to the formation of the PbFe2(AsO_(4))2(OH)2.Batch experiments revealed that Fe/S-BC had outstanding ability to remove As(Ⅲ) and Pb(Ⅱ),regardless of pH dependency and interference by various coexisting ions.The maximum adsorption capacities of the Fe/S-BC for As(Ⅲ) and Pb(Ⅱ) were 91.2 mg/g and 631.7 mg/g,respectively.Fe/S-BC could be treated as a novel candidate for the elimination of As(Ⅲ)-Pb(Ⅱ) combined pollution. | Yijun Chen Qintie Lin Xiaoqing Wen Jin He Haoyu Luo Quanfa Zhong Libin Wu Jiaqi Li | 2023 | Journal of Environmental Sciences2023，，3： | 1 |
| 6 | Improved spa- tial learning performance of fat - 1 mice is associated with enhanced neurogenesis and neuritogenesis by docosahexae- noic acid 显示文摘 | HE Chengwei QU Xiying CUI Libin | 2009 | PNAS2009，106，11： | 1 |
| 7 | Geochemistry and environmental effect of cadmium in the super-large Jinding Pb-Zn deposit,Yunnan Province, China显示文摘The Jinding Pb-Zn deposit in Yunnan Province is the representative of a Cd-enriched area and mining activities lead to the release of Cd into the hypergenic ecosystem, resulting in Cd pollution. The concentrations of Cd vary greatly from one type to another type of rocks in the mining district. In the host rock, Cd concentrations range from 50×10-6 to 650×10-6 with an average of 310×10-6. In primary ores, Cd concentrations range from 14×10-6 to 2800×10-6 with an average of 767×10-6. However, in oxidized ores, Cd concentrations are highest, varying within the range of 110×10-6 to 8200×10-6 , averaging 1661×10-6. It is shown that the oxidized ores are the main carrier and environmental source of Cd. Leaching test showed that Pb/Zn ores are easy to oxidize and thereafter release Cd and other harmful elements. These leached elements in the leachate may be precipitated rapidly in the order of Zn>Pb>Cd. As for the concentration distribution of Cd in the Bijiang River, it is estimated to be 15.7 μg/L Cd in water, 49.3 mg/L in suspended substances, and 203.7 mg/L in sediments. The average value of Cd in soil from the polluted area is 83.0 mg/kg. Natural weathering of Cd-rich rocks and minerals imposes a potential environmental risk on the aquatic ecosystem of the Bijiang catchment. | LI Hang LI Dahua XIAO Tangfu HE Libin NING Zengping SUN Jialong ZHU Changsheng SHUANG Yan | 2008 | Chinese Journal Of Geochemistry2008，27，1： | 1 |
| 8 | Transcriptome analysis of the roots at early and late seedling stages using Illumina paired-end sequencing and development of EST-SSR markers in radish显示文摘 | Shufen Wang Xiufeng Wang Qiwei He Xianxian Liu Wenling Xu Libin Li Jianwei Gao Fengde Wang | 2012 | Plant Cell Reports2012，，8： | 1 |
| 9 | Effect of moxibustion on autophagy and the inflammatory response of synovial cells in rheumatoid arthritis model rats显示文摘OBJECTIVE:To investigate the effect of moxibustion on synovitis and the autophagy of synoviocytes in rheumatoid arthritis(RA).METHODS:Forty Sprague-Dawley rats were randomly divided into a normal group,model group,moxibustion group,cigarette moxibustion group,and medicine group,with eight rats included in each group.The RA model was established by subcutaneous injection of complete Freund's adjuvant into the left posterior toe.Rats in the model group were not interfered with.In the moxibustion group,rats were treated by moxibustion,where a 1-cm diameter moxa stick was applied at the left Zusanli(ST 36)point.The distance of the moxa stick to the skin was 2 cm and moxibustion was completed for 20 min daily for 15 d total.In the cigarette moxibustion group,the moxa stick was replaced by a common cigarette.In the medicine group,rats were treated with a tripterygium glycoside suspension(8 mg/kg)once a day for 15 d total.In each group,the left hind limb toe volume was measured with a toe volume meter;the synovial cells were observed by hematoxylin and eosin staining;the interleukin(IL)-4,IL-6,IL-10,IL-1β,IL-23,IL-17,and tumor necrosis factor(TNF)-αlevels in serum were measured by enzyme-linked immunosorbent assay;the erythrocyte sedimentation rate(ESR)were detected by Westergren sedimentation rate testing;the C-reactive protein(CRP)and rheumatoid factor(RF)levels in serum were detected by rate nephelometry;the expression levels of ULK1,autophagy-associated protein(Atg)3,Atg5,and Atg12 messenger RNA(m RNA)in synovium were detected by real time-quantitative polymerase chain reaction(RT-q PCR);and the protein expression levels of phosphatidylinositol-3-kinase(PI3K),protein kinase B(Akt),mammalian target of rapamycin(mTOR),LC3-Ⅱ,beclin-1,phosphorylated-PI3K(p-PI3K),p-Akt,p-mTOR in synovium were detected by Western blotting.RESULTS:Among the RA model rats,joint swelling,an inflammatory reaction,and the proliferation of synovial tissue were obvious and the signal of the PI3K/Akt/mTOR pathway was active,while autophagy was inhibited.Moxibustion at Zusanli(ST36)or intragastric administration of Tripterygium wilfordii glycosides could alleviate the inflammatory reaction of RA rats;relieve the swelling of the toes;downregulate the levels of ESR,CRF,RF;lower the levels of IL-6,IL-1β,TNF-α,and IL-17;and increase the IL-4 and IL-10.At the same time,the m RNA expression levels of ULK1,Atg3,Atg5,and Atg12 and those of LC3-Ⅱand beclin-1 were increased,while the PI3K,Akt,mTOR,p-PI3K,p-Akt,p-mTOR were decreased.Cigarette moxibustion did not significantly reduce the swelling of the toe joint in RA rats,and was not as good as that of moxibustion or Tripterygium wilfordii polyglycosides in the effects of inflammation relief and the influences of the levels of ESR,CRF,RF.While cigarette moxibustion has a weak effect to affect the expression of corresponding molecules in autophages and the expression level of the autophagy biomaker in synovial tissue.Moxibustion and tripterygium glycosides can significantly reduce the joint swelling,relieve synovitis and synovial hyperplasia,and inhibit the PI3K/Akt/mTOR signaling pathway to increase autophagy in a manner superior to cigarette moxibustion.CONCLUSION:Moxibustion can limit the proliferation of synoviocytes in RA rats by inhibiting the PI3K/Akt/mTOR signaling pathway,promoting autophagy,effectively reducing synovitis,and alleviating joint swelling. | HAO Feng WANG Qiang LIU Lei WU Libin CAI Ronglin SANG Jiajia HU Jun WANG Jie YU Qing HE Lu SHEN Yingchao MIAO Yiming HU Ling WU Zijian | 2022 | Journal of Traditional Chinese Medicine2022，42，1： | 1 |
| 10 | H7N9 Influenza Viruses Are Transmissible in Ferrets by Respiratory Droplet显示文摘 | Qianyi Zhang Jianzhong Shi Guohua Deng Jing Guo Xianying Zeng Xijun He Huihui Kong Chunyang Gu Xuyong Li Jinxiong Liu Guojun Wang Yan Chen Liling Liu Libin Liang Yuanyuan Li Jun Fan Jinliang Wang Wenhui Li Lizheng Guan Qimeng Li Huanliang Yang Pucheng Che | 2013 | Science2013，，6144： | 1 |
| 11 | Improved spatial learn- ing performance of fat-1 mice is associated with enhanced neuro- genesis and neuritogenesis by docosahexaenoic acids 显示文摘 | Chengwei HE Xiying QU Libin CUI | 2009 | PNAS2009，106，11： | 1 |
| 12 | A novel porcine circovirus-like agent P1is associated with wasting syndromes in pigs显示文摘 | LIBIN WEN KONGWANG HE QI XIAO | 2012 | Plos one2012，7，41： | 1 |
| 13 | Complete genome sequence of a novel porcine circovirus-like agent显示文摘 | LIBIN WEN KONGWANG HE ZHENGYU YU | 2012 | J Virol2012，86，： | 1 |
| 14 | Genome-wide unbalanced expression bias and expression level dominance toward Brassica oleracea in artificially synthesized intergeneric hybrids of Raphanobrassica显示文摘Raphanobrassica(R^(r)R^(r)C^(r)C^(r),2n=4x=36),which is generated by distant hybridization between the maternal parent Raphanus sativus(R s R s,2n=2x=18)and the paternal parent Brassica oleracea(C°C°,2n=2x=18),displays intermediate silique phenotypes compared to diploid progenitors.However,the hybrid shares much more similarities in silique phenotypes with those of B.oleracea than those of R.sativus.Strikingly,the silique of Raphanobrassica is obviously split into two parts.To investigate the gene expression patterns behind these phenomena,transcriptome analysis was performed on the upper,middle,and lower sections of pods(RCsiu,RCsim,and RCsil),seeds in the upper and lower sections of siliques(RCseu and RCsel)from Raphanobrassica,whole pods(Rsi and Csi)and all seeds in the siliques(Rse and Cse)from R.sativus and B.oleracea.Transcriptome shock was observed in all five aforementioned tissues of Raphanobrassica.Genome-wide unbalanced biased expression and expression level dominance were also discovered,and both of them were toward B.oleracea in Raphanobrassica,which is consistent with the observed phenotypes.The present results reveal the global gene expression patterns of different sections of siliques of Raphanobrassica,pods,and seeds of B.oleracea and R.sativus,unraveling the tight correlation between global gene expression patterns and phenotypes of the hybrid and its parents. | Libin Zhang Jianjie He Hongsheng He Jiangsheng Wu Maoteng Li | 2021 | Horticulture Research2021，8，1： | 1 |
| 15 | A study on varia- tions of physiological indices of cold resistance in fukiencypres 显示文摘 | He Kaiyue Li Xiaochu Huang Libin | 2002 | Journal of Nanjing Forestry University: Natural Sciences E- dition2002，26，5： | 1 |
| 16 | Environmental exposure of thallium and potential health risk in areas of high natural concentrations of thallium:southwest Guizhou,China显示文摘 | Xiao Tangfu He Libin Guha Jayanta | 2005 | WIT Transactions on Ecology and Environment2005，85，： | 1 |
| 17 | Genetic Diversity of Intragenomic Rearrangement of Porcine Circovirus Type 2 in vitro and in vivo显示文摘We characterized the genome sequences of defective-interfering(DI) particle DNA of porcine circovirus type 2(PCV2) by sequencing and bioinformatics analyses. DI particles were both generated by serial passage of PCV2 in PK15 cells and obtained from sera of pigs with postweaning multisystemic wasting syndrome(PMWS). These subviral isolates ranged from 358 nt to 1 125 nt genomes. Investigating the complexity and diversity of PCV2 DI in vivo and in vitro can help elucidating the evolutionary history of PCV2. | Libin WEN Fengzhi WANG Bin LI Yang YU Zhengyu YU Aihua MAO Jianping XIE Kong-wang HE | 2013 | Agricultural Science & Technology2013，14，12： | 1 |
| 18 | Pathogenic Effects of Cloned Genomic DNA of Porcine Circovirus-like Virus P1 on Neonatal Mice via Different Inoculation Routes显示文摘[Objective] The paper was to explore the pathogenicity of cloned genomic DNA of porcine circovirus-like virus P1 to neonatal mice via different inoculation routes(brain, liver and muscle). [Method] Cloned genomic DNA of P1 was inoculated to neonatal mice via different routes of brain, liver and muscle. Tissues of heart, liver, spleen, lung, kidney and brain were taken from neonatal mice at 7, 14 and 21 d post inoculation, re-spectively. P1 in various tissues were qualitatively and quantitatively detected by using ordinary PCR and quantitative real-time PCR. Meanwhile,histopathological changes were analyzed. [Result] P1 was detected in neonatal mice inoculated through three different routes. The viral load of tis-sues at 7 d post inoculation was significantly higher than those at 14 and 21 d post inoculation. Moreover, muscle inoculation led to the highest vi-ral load in all tissues of neonatal mice. [Conclusion] P1 infection caused different degrees of pathological damage to heart, liver, lung, kidney and brain in neonatal mice. | Sheng Shaoyang Ren Zili Wen Libin He Kongwang Zhu Xuejiao | 2018 | Animal Husbandry and Feed Science2018，10，3： | 0 |
| 19 | 40 Hz light flickering promotes sleep through cortical adenosine signaling显示文摘Flickering light stimulation has emerged as a promising non-invasive neuromodulation strategy to alleviate neuropsychiatric disorders.However,the lack of a neurochemical underpinning has hampered its therapeutic development.Here,we demonstrate that light flickering triggered an immediate and sustained increase(up to 3 h after flickering)in extracellular adenosine levels in the primary visual cortex(V1)and other brain regions,as a function of light frequency and intensity,with maximal effects observed at 40 Hz frequency and 4000 lux.We uncovered cortical(glutamatergic and GABAergic)neurons,rather than astrocytes,as the cellular source,the intracellular adenosine generation from AMPK-associated energy metabolism pathways(but not SAM-transmethylation or salvage purine pathways),and adenosine efflux mediated by equilibrative nucleoside transporter-2(ENT2)as the molecular pathway responsible for extracellular adenosine generation.Importantly,40 Hz(but not 20 and 80 Hz)light flickering for 30 min enhanced non-rapid eye movement(non-REM)and REM sleep for 2–3 h in mice.This somnogenic effect was abolished by ablation of V1(but not superior colliculus)neurons and by genetic deletion of the gene encoding ENT2(but not ENT1),but recaptured by chemogenetic inhibition of V1 neurons and by focal infusion of adenosine into V1 in a dose-dependent manner.Lastly,40 Hz light flickering for 30 min also promoted sleep in children with insomnia by decreasing sleep onset latency,increasing total sleep time,and reducing waking after sleep onset.Collectively,our findings establish the ENT2-mediated adenosine signaling in V1 as the neurochemical basis for 40 Hz flickering-induced sleep and unravel a novel and non-invasive treatment for insomnia,a condition that affects 20%of the world population. | Xuzhao Zhou Yan He Tao Xu Zhaofa Wu Wei Guo Xi Xu Yuntao Liu Yi Zhang Huiping Shang Libin Huang Zhimo Yao Zewen Li Lingya Su Zhihui Li Tao Feng Shaomin Zhang Olivia Monteiro Rodrigo A.Cunha Zhi-Li Huang Kang Zhang Yulong Li Xiahong Cai Jia Qu Jiang-Fan Chen | 2024 | Cell Research2024，34，3： | 0 |
| 20 | Study on the Function of ORF Genes of Porcine Circovirus-like Virus P1显示文摘[Objectives]This study was conducted to determine the functions of eight ORF genes of porcine circovirus-like virus P1.[Methods]The double-copy tandem molecular cloning of porcine circovirus-like virus P1 genome was used to construct molecular clones with eight ORFs deleted by DNA site-directed mutagenesis technology.After transfected into PK15 cells for a certain period of time,RNA were extracted and was used to verify whether the eight ORFs were deleted or not and used for gene microarry analysis.The GO functions and KEGG pathway enrichment of differentially expressed genes were analyzed.[Results]P1 ORF1 is mainly involved in the biological processes of defense response to virus,signal transduction,regulation of Rab GTPase activity,and lipid metabolic process,and involved in the molecular functions of protein phosphatase inhibitor activity,phosphatidylinositol phospholipase C activity,2 iron,2 sulfur cluster binding,phosphoric diester hydrolase activity,and Rab GTPase activator activity,and in the KEGG pathways of secretion of digestive gland and nervous system development.P1 ORF2 is mainly involved in the biological processes of positive regulation of leukocyte chemotaxis,positive regulation of cell proliferation,positive regulation of cell migration,defense response to virus,regulation of cell growth,and involved in the molecular functions of insulin-like growth factor binding,and chemokine activity,and in the KEGG pathways of cytosolic DNA-sensing pathway,RIG-I-like receptor signaling pathway,toll-like receptor signaling pathway,chemokine signaling pathway,and cytokines,cytokine-cytokine receptor interaction.The biological processes,molecular functions and related pathways involving P1 ORF3 and ORF5 are basically similar to those of ORF2.P1 ORF8 is mainly involved in the biological processes of purine ribonucleotide biosynthetic process,amino acid transport,defense response to virus,amino acid transmembrane transport,and involved in molecular functions of N6-(1,2-dicarboxyethyl)AMP AMP-lyase(fumarate-forming)activity,iron-sulfur cluster binding,amino acid transmembrane transporter activity.[Conclusions]The analysis of the ORF functions of P1 virus lays a foundation for the study of its pathogenicity and pathogenesis. | Libin WEN Xuejiao ZHU Qi XIAO Wei WANG Kongwang HE | 2021 | Agricultural Biotechnology2021，10，2： | 0 |
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