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| 1 | TRIM35 mediates protection against influenza infection by activating TRAF3 and degrading viral PB2显示文摘Tripartite motif(TRIM)family proteins are important effectors of innate immunity against viral infections.Here we identified TRIM35 as a regulator of TRAF3 activation.Deficiency in or inhibition of TRIM35 suppressed the production of type I interferon(IFN)in response to viral infection.777m35-deficient mice were more susceptible to influenza A virus(IAV)infection than were wild-type mice.TRIM35 promoted the RIG-Imediated signaling by catalyzing Lys63-linked polyubiquitination of TRAF3 and the subsequent formation of a signaling complex with VISA and TBK1.IAV PB2 polymerase countered the innate antiviral immune response by impeding the Lys63-linked polyubiquitination and activation of TRAF3.TRIM35 mediated Lys48-linked polyubiquitination and proteasomal degradation of IAV PB2,thereby antagonizing its suppression of TRAF3 activation.Our in vitro and in vivo findings thus reveal novel roles of TRIM35,through catalyzing Lys63-or Lys48-linked polyubiquitination,in RIG-I antiviral immunity and mechanism of defense against IAV infection. | Nan Sun Li Jiang Miaomiao Ye Yihan Wang Guangwen Wang Xiaopeng Wan Yuhui Zhao Xia Wen Libin Liang Shujie Ma Liling Liu Zhigao Bu Hualan Chen Chengjun Li | 2020 | Protein & Cell2020，11，12： | 4 |
| 2 | Volatile components,total phenolic compounds,and antioxidant capacities of worm-infected Gomphidius rutilus显示文摘This study evaluated the effects of worm infection on the volatile components,total phenolic compounds,and antioxidant capacities of Gomphidius rutilus.G.rutilus without worms(GW),G.rutilus infected by a small amount of worms(GS;infected area<50%),and G.rutilus infected by a large amount of worms(GL;infected area>50%)were investigated.The volatile components of G.rutilus were analyzed by simultaneous distillation–extraction(SDE)and headspace solid-phase microextraction(HS-SPME)using gas chromatography–mass spectrometry(GC–MS).A total of 17 and 19 types of volatile compounds were detected,including ketones,alcohols,benzene,alkenes,aldehydes,esters,acids,and alkanes.Alcohols comprised the most abundant compound in GL,GS,and GW.The relative content of 1-octen-3-ol was the highest in all mushrooms.The concentration of eight-carbon(C8)compounds relative to the total volatile compounds varied widely,ranging from 40%(GW)to 64.34%(GS)and 84.42%(GS)and to 91.59%(GL),respectively,among the three samples.The antioxidant capability and the total phenolic contents of G.rutilus were evaluated in this study.The highest total phenolic content(TPC)of 192.23 mg GAE/g was found in GL,which differed significantly(P<0.05)from the latter two samples,whereas the lowest value of 156.11 mg GAE/g was found in GW.ABTS radical cation scavenging activity,FRAP ferric reducing antioxidant capacity(FRAP)radical scavenging activity,and oxygen radical absorbance capacity(ORAC)were investigated to screen the antioxidant properties of extracts.The contents of total phenolic compounds and their antioxidant capacities in vitro showed significant correlations(P<0.01).Among the three types of samples,the phenolic compounds of GL exhibited the highest antioxidant capacity,showing the values of 0.089 mM TE/g for ABTS,0.949 mM Fe^2+E/g for FRAP,and 1.952 M TE/g for ORAC.However,regarding the total antioxidant capacity,GS exhibited the highest antioxidant capacity,showing the values of 0.002648 mM TE/g for ABTS,0.004437 mM Fe^2+E/g for FRAP,and 0.256μM TE/g for ORAC.In conclusion,HS-SPME was more suitable for the extraction of volatile aroma components from G.rutilus.GL had the most abundant aroma components.GL had the highest TPC and antioxidant capacity compared with those of GS and GW,whereas GS showed the opposite results.Interestingly,GS was found to have the highest total antioxidant capacity in vitro.Based on these measured indicators,worm infection had no negative effect on the quality of G.rutilus.Therefore,worm-infected G.rutilus can also be consumed by humans. | Libin Sun Wei He Guang Xin Pengju Cai Yin Zhang Zhiyong Zhang Yunyun Wei Bingxin Sun Xiaowen Wen | 2018 | Food Science and Human Wellness2018，7，2： | 2 |
| 3 | Genotyping of porcine circovirus type 2 from a variety of clinical conditions in China显示文摘 | Libin Wen Xin Guo Hanchun Yang | 2005 | Veterinary Microbiology2005，110，： | 1 |
| 4 | Simultaneous adsorption of As(Ⅲ) and Pb(Ⅱ) by the iron-sulfur codoped biochar composite:Competitive and synergistic effects显示文摘Simultaneous elimination of As(Ⅲ) and Pb(Ⅱ) from wastewater is still a great challenge.In this work,an iron-sulfur codoped biochar (Fe/S-BC) was successfully fabricated in a simplified way and was applied to the remediate the co-pollution of As(Ⅲ) and Pb(Ⅱ).The positive enthalpy indicated that the adsorption in As-Pb co-pollution was an endothermic reaction.The mechanism of As(Ⅲ) removal could be illustrated by surface complexation,oxidation and precipitation.In addition to precipitation and complexation,the elimination mechanism of Pb(Ⅱ) also contained ion exchange and electrostatic interactions.Competitive and synergistic effects existed simultaneously in the co-contamination system.The suppression of As(Ⅲ) was ascribed to competitive complexation of the two metals on Fe/S-BC,while the synergy of Pb(Ⅱ) was attributed to the formation of the PbFe2(AsO_(4))2(OH)2.Batch experiments revealed that Fe/S-BC had outstanding ability to remove As(Ⅲ) and Pb(Ⅱ),regardless of pH dependency and interference by various coexisting ions.The maximum adsorption capacities of the Fe/S-BC for As(Ⅲ) and Pb(Ⅱ) were 91.2 mg/g and 631.7 mg/g,respectively.Fe/S-BC could be treated as a novel candidate for the elimination of As(Ⅲ)-Pb(Ⅱ) combined pollution. | Yijun Chen Qintie Lin Xiaoqing Wen Jin He Haoyu Luo Quanfa Zhong Libin Wu Jiaqi Li | 2023 | Journal of Environmental Sciences2023，，3： | 1 |
| 5 | Uncertainty evaluation for bearing fatigue property of CFRP double-lap,single-bolt joints显示文摘The considerable uncertainty in mechanical properties of composite bolted joints not only prevents advanced composite materials from efficient applications,but also threatens the safety and reliability of the aircraft structures.In this paper,the uncertainty in bearing fatigue properties of a CFRP double-lap,single-bolt joint was evaluated by combing a Progressive Fatigue Damage Model(PFDM)with the interval analysis method.In the PFDM,a residualstrain-based gradual material degradation model and a strain-based fatigue failure criterion were combined with a micromechanics-based sudden material degradation model to predict fatigue properties of the joint.Based on the interval analysis,the key uncertain parameters,which were firstly picked out from eighteen structural parameters of the joint,were described by estimated intervals,and the envelope cases were determined to estimate the lower and upper bounds of fatigue properties of the joint.The predicted results have the same tendency with the experimental results in literatures,which indicates that the PFDM combined with the interval analysis shows potential in efficiently evaluating the fatigue reliability of the complex bolted joints with an adequate accuracy. | Meijuan SHAN Fengrui LIU Wen YANG Libin ZHAO Jianyu ZHANG | 2022 | Chinese Journal of Aeronautics2022，35，3： | 1 |
| 6 | Genotyping of porcine circovirus type 2 from a variety of clinical conditions in China显示文摘 | Libin Wen Xin Guo Hanchun Yang | 2005 | Veterinary Microbiology2005，，1： | 1 |
| 7 | Genotyping of porcine circovirus type 2 from a variety of clinical conditions in China显示文摘 | Libin Wen Xin Guo Hanchun Yang | 2005 | Veterinary Microbiology2005，，1： | 1 |
| 8 | Genotyping of porcine circovirus type 2 from a variety of clinical conditions in China显示文摘 | Libin Wen Xin Guo Hanchun Yang | 2005 | Veterinary Microbiology2005，，1： | 1 |
| 9 | A novel porcine circovirus-like agent P1is associated with wasting syndromes in pigs显示文摘 | LIBIN WEN KONGWANG HE QI XIAO | 2012 | Plos one2012，7，41： | 1 |
| 10 | Complete genome sequence of a novel porcine circovirus-like agent显示文摘 | LIBIN WEN KONGWANG HE ZHENGYU YU | 2012 | J Virol2012，86，： | 1 |
| 11 | Equivalent continuum modeling of beam-like truss structures with flexible joints显示文摘The paper investigated the equivalent continuum modeling of beam-like repetitive truss structures considering the flexibility of joints,which models the contact between the truss member and joint by spring-damper with six directional stiffnesses and dampings.Firstly,a two-node hybrid joint-beam element was derived for modeling the truss member with flexible end joints,and a condensed model for the repeating element with flexible joints was obtained.Then,the energy equivalence method was adopted to equivalently model the truss structure with flexible joints and material damping as a spatial viscoelastic anisotropic beam model.Afterwards,the equations of motion for the equivalent beam model were derived and solved analytically in the frequency domain.In the numerical studies,the correctness of the presented method was verified by comparisons of the natural frequencies and frequency responses evaluated by the equivalent beam model with the results of the finite element method model. | Fushou Liu Libin Wang Dongping Jin Hao Wen | 2019 | Acta Mechanica Sinica2019，35，5： | 1 |
| 12 | Genetic Diversity of Intragenomic Rearrangement of Porcine Circovirus Type 2 in vitro and in vivo显示文摘We characterized the genome sequences of defective-interfering(DI) particle DNA of porcine circovirus type 2(PCV2) by sequencing and bioinformatics analyses. DI particles were both generated by serial passage of PCV2 in PK15 cells and obtained from sera of pigs with postweaning multisystemic wasting syndrome(PMWS). These subviral isolates ranged from 358 nt to 1 125 nt genomes. Investigating the complexity and diversity of PCV2 DI in vivo and in vitro can help elucidating the evolutionary history of PCV2. | Libin WEN Fengzhi WANG Bin LI Yang YU Zhengyu YU Aihua MAO Jianping XIE Kong-wang HE | 2013 | Agricultural Science & Technology2013，14，12： | 1 |
| 13 | Pathogenic Effects of Cloned Genomic DNA of Porcine Circovirus-like Virus P1 on Neonatal Mice via Different Inoculation Routes显示文摘[Objective] The paper was to explore the pathogenicity of cloned genomic DNA of porcine circovirus-like virus P1 to neonatal mice via different inoculation routes(brain, liver and muscle). [Method] Cloned genomic DNA of P1 was inoculated to neonatal mice via different routes of brain, liver and muscle. Tissues of heart, liver, spleen, lung, kidney and brain were taken from neonatal mice at 7, 14 and 21 d post inoculation, re-spectively. P1 in various tissues were qualitatively and quantitatively detected by using ordinary PCR and quantitative real-time PCR. Meanwhile,histopathological changes were analyzed. [Result] P1 was detected in neonatal mice inoculated through three different routes. The viral load of tis-sues at 7 d post inoculation was significantly higher than those at 14 and 21 d post inoculation. Moreover, muscle inoculation led to the highest vi-ral load in all tissues of neonatal mice. [Conclusion] P1 infection caused different degrees of pathological damage to heart, liver, lung, kidney and brain in neonatal mice. | Sheng Shaoyang Ren Zili Wen Libin He Kongwang Zhu Xuejiao | 2018 | Animal Husbandry and Feed Science2018，10，3： | 0 |
| 14 | Study on the Function of ORF Genes of Porcine Circovirus-like Virus P1显示文摘[Objectives]This study was conducted to determine the functions of eight ORF genes of porcine circovirus-like virus P1.[Methods]The double-copy tandem molecular cloning of porcine circovirus-like virus P1 genome was used to construct molecular clones with eight ORFs deleted by DNA site-directed mutagenesis technology.After transfected into PK15 cells for a certain period of time,RNA were extracted and was used to verify whether the eight ORFs were deleted or not and used for gene microarry analysis.The GO functions and KEGG pathway enrichment of differentially expressed genes were analyzed.[Results]P1 ORF1 is mainly involved in the biological processes of defense response to virus,signal transduction,regulation of Rab GTPase activity,and lipid metabolic process,and involved in the molecular functions of protein phosphatase inhibitor activity,phosphatidylinositol phospholipase C activity,2 iron,2 sulfur cluster binding,phosphoric diester hydrolase activity,and Rab GTPase activator activity,and in the KEGG pathways of secretion of digestive gland and nervous system development.P1 ORF2 is mainly involved in the biological processes of positive regulation of leukocyte chemotaxis,positive regulation of cell proliferation,positive regulation of cell migration,defense response to virus,regulation of cell growth,and involved in the molecular functions of insulin-like growth factor binding,and chemokine activity,and in the KEGG pathways of cytosolic DNA-sensing pathway,RIG-I-like receptor signaling pathway,toll-like receptor signaling pathway,chemokine signaling pathway,and cytokines,cytokine-cytokine receptor interaction.The biological processes,molecular functions and related pathways involving P1 ORF3 and ORF5 are basically similar to those of ORF2.P1 ORF8 is mainly involved in the biological processes of purine ribonucleotide biosynthetic process,amino acid transport,defense response to virus,amino acid transmembrane transport,and involved in molecular functions of N6-(1,2-dicarboxyethyl)AMP AMP-lyase(fumarate-forming)activity,iron-sulfur cluster binding,amino acid transmembrane transporter activity.[Conclusions]The analysis of the ORF functions of P1 virus lays a foundation for the study of its pathogenicity and pathogenesis. | Libin WEN Xuejiao ZHU Qi XIAO Wei WANG Kongwang HE | 2021 | Agricultural Biotechnology2021，10，2： | 0 |
| 15 | Indocyanine Green Angiographic and Multifocal Electroretinographic Features in the Diffuse and Regional From of Bietti's Cystalline Retinopathy显示文摘Prpose: To observe different features of indocyanine green angiography(ICGA) andtifocal electroretinography (ERG) in the diffuse and regional type of Bieti′sc stalline retinopathy (BCR). Thods: ICGA and the multifocal ERG were performed in two cases of the diffuse andregional type of BCR respectively. These data were compared with fluoresceinangiography (FA), standard Ganzfeld ERG, and visual field testing. Results: In the regional case, ICGA revealed reduced perfusion of the choroidalcirculation in the early phase and multiple hypofluorescent spots in the posterior pole in the late phase, due to choriocapillaris filling defect; the extent of choroiocapillaris losswas shown in early phase of ICGA and there were multifocal hyperfluorescent dotssurrounding hypofluorescent spots in late phase in the diffuse case. The multifocal ERGshowed that the central responses were markedly depressed, corresponding to the visualfield defects, while the findings of Ganzfeld ERG were normal in the regional BCR;however, both the multifocal ERG and Ganzfeld ERG were severely subnormal in thediffuse case.Conclusions: The features of ICGA and multifocal ERG are different between the diffuseand regional BCR. In the meantime, the two tools are also useful to differentiate the typeand assess the extentof evolution in BCR. | Libin Jiang, Feng Wen, Lezheng Wu, Hong Yan, Shixing HuZhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China | 2002 | Eye Science2002，18，1X： | 0 |
| 16 | TCP-1,a novel peptide to diagnose early colon cancer显示文摘A nine cyclic peptide(TCP-1)showed excellent specificity for colon cancer.TCP-1 binds with human tumor tissues at early stages and mice tumor with diameters of 1-4 mm,suggesting that TCP-1 may be used for early diagnosis of colon cancer.The mechanism of the targeted binding of TCP-1 to colon cancer was also studied using immunoprecipitation,LC-MS and bioinformatics.After screening and identifying of the possible binding target proteins of TCP-1,keratin,typeⅡcytoskeletal 5 was speculated to be the specific binding target protein of TCP-1 in human tumor tissue.Pharmacokinetics studies were conducted to investigate the target-mediated drug disposition of the new tumor-specific peptide by LC-MS/MS.The tissue distribution study showed that TCP-1 was found only in colon tumors(the target site)in tumor mice did not bind to any other tissues.Conjugating TCP-1 to tumor markedly increased its removal rate from blood circulation but mildly extended its staying time in vivo.In tumor mice,a lower AUC of TCP-1(reduced by almost 35%)and 2-fold higher clearance were found compared to that of normal mice.The proposed metabolic pathway of TCP-1 in the kidney was also determined using LC-MS^(n)-IT-TOF.The high specificity and low toxicity of the peptide may be caused by its extremely tight binding to the targets.Potential applications for future clinical use,including MRI and PET/CT were also explored,and this research may promote the development of colon cancer diagnostic technology research and provide new ideas and technical routes for tumor diagnostic technology. | Hang Yu Baoying Wen Min Huang Ru Feng Libin Pan Manyi Xu Hao Lin Lin Cong Sen Zhang Yan Li Chi-Hin Cho Chongjing Zhang Xiaoguang Chen Yan Wang | 2024 | Chinese Chemical Letters2024，35，3： | 0 |
| 17 | The Establishment of Immunochemistry Test Based on a Synthetic Peptide Antibody for the Detection of a Porcine Circovirus-Like Virus P1显示文摘Recently, a novel porcine circovirus-like virus P1 with a circular DNA genome of 0.648 kb was identified. P1 antigen was detected both in vitro and in vivo by synthetic peptide-derived polyclonal antibody-based immunochemistry. The designed peptides were synthesized by solid-phase technique, purified by high performance liquid chromatography, coupled to Keyhole limpet hemocyanin, and injected into rabbits to prepare polyclonal antibody. The emergence of positive cells revealed that synthetic peptide could elicit antibodies against P1 and viral protein could be synthesized. The polyclonal peptide antibodies described here was successfully applied to immunochemical staining and proved helpful in diagnosing P1. | Libin WEN Fengzhi WANG Kongwang HE Yanxiu NI Xuehan ZHANG Rongli GUO Bin LI Xiaomin WANG Zhengyu YU Aihua MAO Junming ZHOU Lixin LU Jianping XIE | 2014 | Agricultural Science & Technology2014，15，2： | 0 |
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