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| 1 | Cloning and expression of MXR7 gene in human HCC tissue显示文摘AIM To clone and identify the whole cDNA ofMXR7 gene and to find out its expression inhuman HCC,and normal tissues.METHODS The DNA primers were designed andsynthesized according to the whole cDNAsequence of MXR? gene.The cDNA of humanHCC was taken as the template while the cDNA ofMXR7 gene was synthesized by polymerasechain reaction(PCR).Recombinant DNAconforming to reading frame was constructed byconnecting purified PCR product of the cDNA ofMXR? gene with expression vector pGEX-5X-1 offusion protein.The plasmid MXRT/pGEX-5X-1was identified by sequencing.Using 32p labeledMXR? cDNA as probe,MXR7 mRNA expressionwas detected by Northern blot analysis in 12different human normal tissues,7 preoperativelyuntreated non-liver tumor tissues,30preoperatively untreated HCC,theparacancerous liver tissues and 12 normal livertissues samples.RESULTS Restriction enzyme and sequenceanalysis confirmed that the insertion sequence invector pGEX-5X-1 was the same as the cDNAsequence of MXR7 gene.Northern blot analysisshowed no expression of MXR? mRNA in 12 kindsof normal human tissues including liver,7 tumortissues in other sites and 12 normal livertissues,the frequencies of MXR7 mRNA expression in HCC and paracancerous livertissues were 76.6% and 13.3%,respectively.The frequency of MXR7 mRNA expression in HCCwithout elevation of serum AFP and in HCC<5cm was 90%(9/10)and 83.3%(5/6),respectively.CONCLUSION MXR7 mRNA is highly expressedin human HCC,which is specific and occurs at anearly stage of HCC,suggesting MXR7 mRNA canbe a tumor biomarker for HCC.The detection ofMXR7 mRNA expression in the biopsied livertissue is helpful in discovering early subclinicalliver cancer in those with negative serum AFP. | Zhou XP Wang HY Yang GS Chen ZJ Li BA Wu MC | 2000 | World Journal of Gastroenterology2000，6，1： | 23 |
| 2 | miR-106b-25/miR-17-92 clusters: Polycistrons with oncogenic roles in hepatocellular carcinoma显示文摘MicroRNAs are small endogenously expressed RNA molecules which are involved in the process of silencing gene expression through translational regulation.The polycistronic miR-17-92 cluster is the first microRNA cluster shown to play a role in tumorigenesis.It has two other paralogs in the human genome,the miR-106b-25 cluster and the miR-106a-363 cluster.Collectively,the microRNAs encoded by these clusters can be further grouped based on the seed sequences into four families,namely the miR-17,the miR-92,the miR-18and the miR-19 families.Over-expression of the miR-106b-25 and miR-17-92 clusters has been reported not only during the development of cirrhosis but also subsequently during the development of hepatocellular carcinoma.Members of these clusters have also been shown to affect the replication of hepatitis B and hepatitis C viruses.Various targets of these microRNAs have been identified,and these targets are involved in tumor growth,cell survival and metastasis.In this review,we first describe the regulation of these clusters by c-Myc and E2F1,and how the members of these clusters inturn regulate E2F1 expression forming an auto-regulatory loop.In addition,the roles of the various members of the clusters in affecting relevant target gene expression in the pathogenesis of hepatocellular carcinoma will also be discussed. | Weiqi Tan Yang Li Seng-Gee Lim Theresa MC Tan | 2014 | World Journal of Gastroenterology2014，20，20： | 17 |
| 3 | Mouse embryos cloned from brain tumors显示文摘Cancer cells escape from growth cont rol by accumulating genetic and epig enetic alterations.In rare instances,epigenetic changes alone are oncogenic.Furthermore,a gents that modify DNA methylation or chromatin structure can restore a normal phenotype to cells harboring oncogenic mutations.How ever,it is unclear to what extent epi genetic reprogramming can reverse o ncogenesis.Using somatic nuclear transfer,we show that medul loblastomas arising in Ptc1+/-mice can direct preimplantation develop ment.Additionally,blastocysts derived from medullobl astoma nuclei form postimplantatio n embryos with typical cell layers.T hus,tumor cells can be epigenetically reprogrammed into n ormal cell types.This approach coul d lead to a general strategy for assessing genetic and epigenetic contributions to tumorigenesis. | Li L Connelly MC Wetmore C Curran T Morgan JI | 2003 | 癌症2003，22，7： | 11 |
| 4 | Microstructure of Al-Si Alloys Rapidly Solidified from the Different Temperature Melts显示文摘Hypo- and hyper-eutectic Al-Si alloys were rapidly solidified from the low or high temperature melt using water quenching and melt-spinning technique. The differences in microstructure of rapid solidification alloys were studied by scanning and transmission electron microscopy,and X-ray diffraction methods. The primary silicon in the Al-22(wt pct)Si alloy quenched in room temperature water from high temperature melt was smaller than that quenched from low temperature melt. Compared with the ribbons spun from low temperature melt, the ribbons spun from high temperature melt had finer Al-rich and Si-rich phase particles in either Al-7(wt pct)Si or Al-18(Wt pct)Si alloy. The mechanism was explained by the nature of the liquid | Gui, MC Jia, J Song, GS Li, QC | 1999 | Journal of Materials Science & Technology1999，15，3： | 7 |
| 5 | Roles of paroxetine and corticosterone on adult mammalian ciliary body cell proliferation显示文摘背景在成年哺乳动物的视网膜的神经发生通常被废除,并且这显示再生 capacity.Despite 的视网膜缺乏这,有在保留学习在调查二药,皮质脂酮和 paroxetine 的效果瞄准了的在场的神经原的 potential.The 的睫的上皮的nestin积极的房间的一张小人口,在老鼠是的睫的 body.Methods 成年人 Sprague-Dawley 的房间增长上给车辆,皮质脂酮, paroxetine ,或两个 | WANG Hua LAU Benson WM YAU Suk-yu LI Suk-yee LEUNG Nelson WANG Ning-li TANG Siu-wa LEE Tatia MC SO Kwok-fai | 2010 | Chinese Medical Journal2010，，10： | 2 |
| 6 | Accelerated maturation of primate testis by xenografting into mice显示文摘 | Honaramooz A Li MW Penedo MC | 2004 | Biol Reprod2004，70，： | 2 |
| 7 | Molecular interactions between telomerase and the tumor suppressor protein p53 in virtro显示文摘 | Li H Cao Y Berodt MC | 1999 | Oncogene1999，18，48： | 1 |
| 8 | Post-traumatic bilateral facial palsy:a case report and literature review显示文摘 | Li J Goldberg G Munin MC | 2004 | Brain Inj2004，18，3： | 1 |
| 9 | Disturbed sleep:Effects of sociocultural factors and illness显示文摘 | Rona RJ Li L Gulliford MC | 1998 | Arch Dis Child1998，78，1： | 1 |
| 10 | Mental Health and Social Support Among HW - Positive Injection Drug Users and Their Caregivers in China显示文摘 | Greene MC Zhang JP Li JP | 2013 | AIDS Behav2013，17，5： | 1 |
| 11 | Echinocosis显示文摘 | Mc Manus Zhang D P W Li J | 2005 | Lancet2005，362，： | 1 |
| 12 | Expression of the apoptosis inhibitor,surviving,in nonmelanoma skin cancer and gene targeting in a keratinocyte cell line显示文摘 | Gross man D MC Niff JM Li F | 1999 | Lab Invest1999，79，： | 1 |
| 13 | Worldwide Nanotech- nology Development: A Comparative Study of USPTO, EPO, and JPO Patents ( 1976 - 2004) 显示文摘 | Li X Lin Y Chen H Roco MC | 2007 | Journal of Nanoparticle Research2007，9，6： | 1 |
| 14 | The traditional Chinese herbal formula ASHMI inhibits allergic lung inflammationin antigen-sensitized and antigen-challenged aged mice显示文摘 | Busse P J Schofield B Birmingham N Yang N Wen MC Zhang T Srivastava K Li XM | | 0，，03： | 1 |
| 15 | Facial emotion processing in schizophremia:a meta-analysis of functional neuroimaging data显示文摘 | Li H Chan RC Mc Alonan GM | 2010 | Schizophr Bull2010，36，5： | 1 |
| 16 | Disturbed sleep:effects of sociocultural factors and illness显示文摘 | RONA RJ LI L GULLIFORD MC | 1998 | Arch Dis Child1998，78，： | 1 |
| 17 | The longitudinal effects of a perimeno-pausal health education intervention on the mid-life women in Taiwan显示文摘 | Tsao LI Su MC Hsiao PJ | 2007 | Maturitas2007，57，3： | 1 |
| 18 | Metabolic syndrome, diabetes, and hyperuricemia显示文摘 | LI C I-Isieh MC Syndrome CM | 2013 | Curt Opin Rheumatol2013，25，2： | 1 |
| 19 | Studies on the role of Dlx5 in regulation of chondrocyte differentiation during endochondral ossification in the developing mouse limb显示文摘 | Chin HJ Fisher MC Li Y | 2007 | Dev Growth Differ2007，49，6： | 1 |
| 20 | Application of PHBHHx nanoparticles as sustained drug release carrier for ra?pamycin显示文摘 | Li MC Zhang YL Lu XY | 2013 | Journal of Xi'' an Jiaotong University (Medical sciences)2013，34，2： | 1 |
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