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| 1 | Cloning and expression of MXR7 gene in human HCC tissue显示文摘AIM To clone and identify the whole cDNA ofMXR7 gene and to find out its expression inhuman HCC,and normal tissues.METHODS The DNA primers were designed andsynthesized according to the whole cDNAsequence of MXR? gene.The cDNA of humanHCC was taken as the template while the cDNA ofMXR7 gene was synthesized by polymerasechain reaction(PCR).Recombinant DNAconforming to reading frame was constructed byconnecting purified PCR product of the cDNA ofMXR? gene with expression vector pGEX-5X-1 offusion protein.The plasmid MXRT/pGEX-5X-1was identified by sequencing.Using 32p labeledMXR? cDNA as probe,MXR7 mRNA expressionwas detected by Northern blot analysis in 12different human normal tissues,7 preoperativelyuntreated non-liver tumor tissues,30preoperatively untreated HCC,theparacancerous liver tissues and 12 normal livertissues samples.RESULTS Restriction enzyme and sequenceanalysis confirmed that the insertion sequence invector pGEX-5X-1 was the same as the cDNAsequence of MXR7 gene.Northern blot analysisshowed no expression of MXR? mRNA in 12 kindsof normal human tissues including liver,7 tumortissues in other sites and 12 normal livertissues,the frequencies of MXR7 mRNA expression in HCC and paracancerous livertissues were 76.6% and 13.3%,respectively.The frequency of MXR7 mRNA expression in HCCwithout elevation of serum AFP and in HCC<5cm was 90%(9/10)and 83.3%(5/6),respectively.CONCLUSION MXR7 mRNA is highly expressedin human HCC,which is specific and occurs at anearly stage of HCC,suggesting MXR7 mRNA canbe a tumor biomarker for HCC.The detection ofMXR7 mRNA expression in the biopsied livertissue is helpful in discovering early subclinicalliver cancer in those with negative serum AFP. | Zhou XP Wang HY Yang GS Chen ZJ Li BA Wu MC | 2000 | World Journal of Gastroenterology2000，6，1： | 23 |
| 2 | Morphometric study of the apical anatomy of C-shaped root canal systems in mandibular second molars显示文摘 | Cheung GS Yang J Fan B | 2007 | Int Endod J2007，40，4： | 1 |
| 3 | Selection of single blastocysts for fresh transfer via standard morphology assessment alone and with array CGH for good prognosis IVF patients: results from a randomized pilot study显示文摘 | Yang Z Liu J Collins GS | 2012 | Mol Cytogenet2012，5，： | 1 |
| 4 | Noncoronary ST eleva- tion and polymorphic ventricular tachycardia during left-sided accessory pathway ablation显示文摘 | Hwang GS Park JS Yang HM | 2013 | J Cardiovasc Electrophysiol2013，24，： | 1 |
| 5 | Morphometric study of the apical anatomy of C-shaped root canal systems in mandibular second molars 显示文摘 | Cheung GS Yang J Fan B | 2007 | Int Endod J2007，40，4： | 1 |
| 6 | Mice lacking Smad3 showaccelerated wound healing and an impaired local inflammatory response 显示文摘 | Ashcroft GS Yang X G|ick AB | 1999 | Nat Cell Biol1999，1，5： | 1 |
| 7 | Morphometric study of the apical a- natomy of C- shaped root canal systems in mandibular second molars显示文摘 | Cheung GS Yang J Fan B | 2007 | lnt Endod J2007，40，4： | 1 |
| 8 | Hepcidin1 knockout mice display defects in bone microarchitecture and changes of bone formation markers显示文摘 | Shen GS Yang Q Jian JL | 2014 | Calcif Tissue Int2014，94，： | 1 |
| 9 | Valproate pretreatment protects dopaminergic neurons from LPS-induced neurotoxicity in rat primary midbrain cultures: role of microglia显示文摘 | Peng GS Li G Tzeng NS Chen PS Chuang DM Hsu YD Yang S Hong JS | 2006 | 中国生物学文摘2006，20，10： | 1 |
| 10 | Selection of single blastoeysts for fresh transfer via standard morphology assessment alone and with array CGH for good prognosis IVF patients: resuhs from a randomized pilot study 显示文摘 | Yang Z Liu J Collins GS | 2012 | Mol Cytogenet2012，5，1： | 1 |
| 11 | Symptom distress and quality of life after stereotactic radiosurgery in patients with pituitary tumors : a questionnaire survey显示文摘 | Yang CJ Huang GS Xiao FR | 2014 | Plos One2014，9，2： | 1 |
| 12 | Determination of extracellular glutathione in lives of anaesthetized rats by microdialysis with on-line high-perfo-rmance liquid chromatography 显示文摘 | Yang GS Tsai PJ Chen WY | 1995 | Journal of Chromatography B1995，667，： | 1 |
| 13 | Survival bene fit with concim itantclo-pidogrel and glycoprotei nⅡb/Ⅱ Iainhibitort hera py at ad hoc percut aneo us coro-nary i nterventi on显示文摘 | Gumina R J Yang EH Sandhu GS | 2008 | Mavo Clin Proc2008，83，： | 1 |
| 14 | Sodium/potassium/calci- um exchanger 3 is regulated by the steroid hormones estrogen and progesterone in the uterus of mice during the estrous cycle 显示文摘 | Yang H Lee GS Yoo YM | 2009 | Biochem Biophys Res Commun2009，385，2： | 1 |
| 15 | Acupuncture alleviates colorectal hypersensitivity and correlates with the regulatory mechanism of Trp V1 and p-ERK显示文摘 | Wang SJ Yang HY Xu GS | 2012 | Evidence-Based Complementary and Alternative Medicine2012，2012，： | 1 |
| 16 | Hepatitis C markets in hemodialysis patients 显示文摘 | Huang GS Ho Ms Yang CS | 1993 | J Clin Microbiol1993，31，： | 1 |
| 17 | Morphometric study of the api-cal anatomy of C-shaped root canal systems in mandibularsecond molars显示文摘 | Cheung GS Yang J Fan B | 2007 | Int Endod J2007，40，4： | 1 |
| 18 | Virus-mediated transduction of murine retina with adeno-associated virus:effects of viral capsid and genome size显示文摘 | Yang GS Schmidt M Yan Z | 2002 | J Virol2002，76，15： | 1 |
| 19 | Comparison of quantitative parameters in cervix cancer measured by dynamic contrast-enhanced MRI and CT显示文摘 | Yang C Stadler WM Karczmar GS | 2010 | Magn Reson Med2010，63，6： | 1 |
| 20 | Liver transplantation outcomes in 1,078 hepatoeellular carcinoma patients: a multi-center experience in Shanghai, China显示文摘 | Fan J Yang GS Fu ZR | 2009 | J Cancer Res Clin Onco12009，135，10： | 1 |
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