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| 1 | Hwangryunhaedoktang exerts anti-inflammation on LPS-induced NO production by suppressing MAPK and NF-κB activation in RAW264.7 macrophages显示文摘OBJECTIVE: This study aimed to evaluate whether Hwangryunhaedoktang(HHT), a herbal compound, has an inhibitory effect on lipopolysaccharide(LPS)-induced inflammation in RAW264.7 macrophages.METHODS: The effects of HHT were evaluated by confirming nitric oxide(NO) production and expression of inducible NO synthase(iN OS) and mitogen-activated protein kinases(MAPKs) in LPS-stimulated RAW264.7 macrophages via the Griess assay, Western blotting, and real-time reverse transcription quantitative polymerase chain reaction. Western blot analyses and luciferase assays were used to evaluate whether HHT has an effect on the phosphorylation and translocation of nuclear factor-κB(NF-κB). The secretion and expression of tumor necrosis factor-α(TNF-α) and interleukin-6(IL-6) were determined via enzyme-linked immunosorbent assay and Western blot analyses.RESULTS: HHT suppressed LPS-induced NO production and expression of iN OS in a dose-dependent manner. Additionally, MAPKs activation was also attenuated via inhibition of phosphorylation of extracellular signal-regulated kinases 1/2, c-Jun N-terminal kinase and p38 which were related to inflammatory pathway. Furthermore, HHT also effectively attenuated NF-κB activation and its translocation to the nucleus, a process that is closely linked to inflammation. LPS normally induced the expression of inflammatory cytokines such as TNF-α and IL-6, but the secretion and expression of TNF-α and IL-6 were significantly attenuated by pretreating the cells with HHT.CONCLUSION: HHT suppressed LPS-induced NO production by blocking the activation of NF-κB and MAPK signaling pathways in RAW264.7 macrophages. Furthermore, HHT may have an anti-inflammatory effect by suppressing the LPS-induced secretion of TNF-α and IL-6. Therefore, the traditional herbal formula HHT might be a useful potential therapeutic agent for inflammation. | Byung Hyuk Han Yun Jung Lee Jung Joo Yoon Eun Sik Choi Seung Namgung Xian Jun Jin Da Hye Jeong Dae Gill Kang Ho Sub Lee | 2017 | Journal of Integrative Medicine2017，15，4： | 5 |
| 2 | Involvement of heme oxygenase-1 induction in anti-vascular inflammation effects of Xanthoceras sorbifolia in human umbilical vein endothelial cells显示文摘OBJECTIVE: To define the effects of Xanthoceras sorbifolia(EXS) on vascular inflammation and the mechanisms in endothelial cells.METHODS: Vascular protective effects of an ethanol extract of seeds from EXS(1-50 μg/mL) against tumor necrosis factor-α(TNF-α)-induced vascularinflammation were examined in human umbilical vein endothelial cells(HUVECs).RESULTS: EXS significantly decreased TNF-α-induced expression of cell adhesion molecules, such as intracellular adhesion molecule-1, vascular cell adhesion molecule-1, and endothelial cell selectin,in a dose-dependent manner. Pre-treatment with EXS significantly inhibited translocation and transcriptional activity of nuclear factor-κB(NF-κB) increased by TNF-α. EXS also significantly inhibited formation of intracellular reactive oxygen species(ROS). Moreover, the vascular protective effects of EXS were linked to up-regulation of heme oxygenase-1(HO-1) and nuclear factor E2-related factor-2(Nrf-2) expression. EXS-induced HO-1 expression was significantly decreased in SnPP(HO-1 inhibitor)-and HO-1 siRNA-treated cells, whereas an increase was found in cobalt protoporphyrin IX(CoPP)(HO-1 inducer)-treated cells. In addition, pretreatment with EXS increased HO-1 and Nrf-2 expression under TNF-α stimulation with or without N-acetyl-L-cysteine. Furthermore, the inhibitory effects of EXS on TNF-α-induced vascular inflammation were partially reversed in SnPP-and of HO-1siRNA-treated cells but increased by CoPP.CONCLUSION: These results suggest that EXS may have important implications for prevention of vascular complications associated with vascular inflammation by inhibition of the NF-κB/ROS pathway and activation of the Nrf-2/HO-1 pathway. | Jung Joo Yoon Byung Hyuk Han Eun Sik Choi Seung Namgung Da Hye Jeong Song Nan Jin Yun Jung Lee Dae Gill Kang Ho Sub Lee | 2018 | Journal of Traditional Chinese Medicine2018，38，6： | 4 |
| 3 | Sinkihwan-gamibang ameliorates puromycin amino nucleoside-induced nephrotic syndrome显示文摘Nephrotic syndrome(NS)is a kidney disease characterized by hypertriglyceridemia,massive proteinuria,hypo-albuminemia and peripheral edema.Sinkihwan-gamibang(SKHGMB)was recorded in a traditional Chinese medical book named“Bangyakhappyeon(方藥合編)”and its three prescriptions Sinkihwan,Geumgwe-sinkihwan,and Jesaeng-sinkihwan belong to Gamibang.This study confirmed the effect of SKHGMB on renal dysfunction in an NS model induced by puromycin aminonucleoside(PAN).The experimental NS model was induced in male Sprague Dawley(SD)rats through injection of PAN(50 mg·kg^(-1))via the femoral vein.SKHGMB not only reduced the size of the kidneys increased due to PAN-induced NS,but also decreased proteinuria and ascites.In addition,SKHGMB significantly ameliorated creatinine clearance,creatinine,and blood urea nitrogen.SKHGMB relieved glomeruli dilation and tubules fibrosis in the glomeruli of the NS model.SKHGMB inhibited the protein and mRNA levels of the NLRP3 inflammasome including NLRP3,ASC,and pro-caspase-1 in NS rats.SKHGMB reduced the protein and mRNA levels of fibrosis regulators in NS rats.The results indicated that SKHGMB exerts protective effects against renal dysfunction by inhibiting of renal inflammation and fibrosis in NS rats. | Lee Hyeon Kyoung Jang Youn Jae Na Se Won Kim Hye Yoom Han Bywig Hyuk Lee Yun Jung Lee Ho Sub Yoon Jung Joo Kang Dae Gill | 2022 | Chinese Journal of Natural Medicines2022，20，3： | 2 |
| 4 | Pathologic Complete Response of Primary Tumor Following Preoperative Chemoradiotherapy for Locally Advanced Rectal Cancer: Long-term Outcomes and Prognostic Significance of Pathologic Nodal Status (KROG 09-01)显示文摘 | Seung-Gu Yeo Dae Yong Kim Tae Hyun Kim Hee Jin Chang Jae Hwan Oh Won Park Doo Ho Choi Heerim Nam Jun-Sang Kim Moon-June Cho Jong Hoon Kim Jin-hong Park Min Kyu Kang Woong Sub Koom Jae-Sung Kim Taek-Keun Nam Eui Kyu Chie Jung Soo Kim Kyung-Ja Lee | 2010 | Annals of Surgery2010，，6： | 1 |
| 5 | Vasodilatory effects of ethanol extract of Radix Paeoniae Rubra and its mechanism of action in the rat aorta显示文摘 | Song Nan Jin Jin Fu Wen Ting Ting Wang Dae Gill Kang Ho Sub Lee Kyung Woo Cho | 2012 | Journal of Ethnopharmacology2012，，1： | 1 |
| 6 | Prevalence of Inducible Clindamycin resistance in Staphylococcal Isolates at a Korean Tertiary Care Hospital显示文摘 | Hwan Sub Lim Hyukmin Lee Kyoung Ho Roh | | 0，，04： | 1 |
| 7 | Dose effect of cigarette smoking on frequency and spectrum of epidermal growth factor receptor gene mutations in Korean patients with non-small cell lung cancer显示文摘 | Young Joo Lee Hyo Sub Shim Young Ae Kang Su Jung Hong Hyun Ki Kim Hoguen Kim Se Kyu Kim Sung Ho Choi Joo-Hang Kim Byoung Chul Cho | 2010 | Journal of Cancer Research and Clinical Oncology2010，，12： | 1 |
| 8 | Bradykinin-induced expression of α-smooth muscle actin in human mesenchymal stem cells显示文摘 | Young Mi Kim Eun Su Jeon Mi Ra Kim Jung Sub Lee Jae Ho Kim | 2008 | Cellular Signalling2008，，10： | 1 |
| 9 | Endoscopic closure of a distal common bile duct perforation caused by papillary dilation with a large-diameter balloon (with video)显示文摘 | Yong Sub Lee Jong Ho Moon Bong Min Ko Hyun Jong Choi Young Deok Cho Sang Heum Park Moon Sung Lee Chan Sup Shim | 2010 | Gastrointestinal Endoscopy2010，，3： | 1 |
| 10 | A Phase II Study of Synchronous Three-Dimensional Conformal Boost to the Gross Tumor Volume for Patients With Unresectable Stage III Non–Small-Cell Lung Cancer: Results of Korean Radiation Oncology Group 0301 Study显示文摘 | Kwan Ho Cho Sung Ja Ahn Hong Ryull Pyo Kyu-Sik Kim Young-Chul Kim Sung Ho Moon Ji-Youn Han Heung Tae Kim Woong Sub Koom Jin Soo Lee | 2009 | International Journal of Radiation Oncology Biology Physics2009，，5： | 1 |
| 11 | Beneficial Effect of Berberis amurensis Rupr. on Penile Erection显示文摘Objective: To investigate whether the methanol extract of Berberis amurensis Rupr.(BAR) augments penile erection using in vitro and in vivo experiments. Methods: The ex vivo study used corpus cavernosum strips prepared from adult male New Zealand White rabbits. In in vivo studies for intracavernous pressure(ICP), blood pressure, mean arterial pressure(MAP), and increase of peak ICP were continuously monitored during electrical stimulation of Sprague-Dawley rats. Results: Preconstricted with phenylephrine(PE) in isolated endotheliumintact rabbit corus cavernosum, BAR relaxed penile smooth muscle in a dose-dependent manner, which was inhibited by pretreatment with NG-nitro-L-arginine methyl ester(L-NAME), a nitric oxide synthase inhibitor, and 1 H-[1,2,4]-oxadiazole-[4,3-α]-quinoxalin-1-one, a soluble guanylyl cclase inhibitor. BAR significantly relaxed penile smooth muscles dose-dependently in ex vivo, and this was inhibited by pretreatment with L-NAME 1 H-[1,2,4]-oxadiazole-[4,3-α]-quinoxalin-1-one. BAR-induced relaxation was significantly attenuated by pretreatment with tetraethylammonium(TEA, P<0.01), a nonselective K+ channel blocker, 4-aminopyridine(4-AP, P<0.01), a voltage-dependent K+ channel blocker, and charybdotoxin(P<0.01), a large and intermediate conductance Ca2+sensitive-K+ channel blocker, respectively. BAR induced an increase in peak ICP, ICP/MAP ratio and area under the curve dose dependently. Conclusion: BAR augments penile erection via the nitric oxide/cyclic guanosine monophosphate system and Ca2+ sensitive-K+(BKCa and IKCa) channels in the corpus cavernosum. | Rui Tan Yun Jung Lee Kyung Woo Cho Dae Gill Kang Ho Sub Lee | 2018 | Chinese Journal of Integrative Medicine2018，24，6： | 0 |
| 12 | Two distinct stem cell-like subtypes of hepatocellular carcinoma with clinical significance and their therapeutic potentials显示文摘Dear Editor Hepatocellular carcinoma(HCC)is among the most com-mon cancers worldwide,causing about 600,000 deaths annully[1].In HCC,stem cell-like characteristics,which drive early recurrence and therapy resistance,are major contributors to poor prognosis[2].In this current study,we integrated and analyzed gene expression data from human fetal liver cells and primary HCC tumors(n=1231)and.uncovered two clinically and biologically distinct hepatic stem cell(HS)subtypes,potential biomarkers associated with these subtypes,and a potential new therapeutic inter-vention for these subtypes. | Sung Hwan Lee Yun Seong Jeong Sunyoung Lee Bo Hwa Sohn Ho Kyoung Hwang Gi Hong Choi Chang Moo Kang Jin Sub Choi Woo Jung Lee Jae-Ho Cheong Hee Jin Jang Ahmed Kaseb Lewis Roberts Sun Young Yim Yun Shin Chun Ju-Seog Lee | 2022 | Cancer Communications2022，42，2： | 0 |
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