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| 1 | Prevalence of HIV Indeterminate Western Blot Tests and Follow-up of HIV Antibody Sero-Conversion in Southeastern China显示文摘HIV-indeterminate Western blotting(WB)results are typically obtained in WB confirmatory assays,and the number of indeterminate samples may increase with the detection of HIV infections,which will present considerable challenges for the management of HIV/AIDS.Nucleic acid detection has been used as a laboratory test for screening suspected or indeterminate samples.However,the effectiveness of these assays for the differential diagnosis of HIV-indeterminate WB samples remained undetermined.In this study,210 subjects with HIV-indeterminate WB results were detected from 6360 positive HIV screening samples between 2015 and 2016 in southeastern China,in which HIV-indeterminate WB results accounted for 3.30%.The highest proportion of indeterminate results was observed in pregnant and lying-in women receiving physical examinations(16.67%),followed by that in voluntary blood donors(8.82%).The most common WB band patterns were p24,gpl60 and p24,and gpl60.The follow-up study revealed that the highest negative and positive conversion rates of HIV antibodies were in samples with a single p24 band(80.28%),and with gpl60 and p24 bands(86.21%),respectively.Among the Env,Gag,and Pol antibodies,samples with a Gag band showed the highest negative conversion rate(81.25%),whereas the highest positive conversion rate was observed in samples with an Env band(56.76%).In addition,quantitative and qualitative HIV nucleic acid testing exhibited the highest sensitivity(96.3%)and specificity(97.85%),respectively.Our results indicate a lower proportion of HIV indeterminate WB results in southeastern China compared to previous reports,and the follow-up re-examination of patients with HIV indeterminate results should be performed.Nucleic acid testing facilitates the identification of HIV infections. | Shouli Wu Min Gao Jian Zheng Pingping Yan Wei Wang Xiaoli Lu Yuefeng Qiu Yansheng Yan | 2019 | Virologica Sinica2019，34，4： | 15 |
| 2 | Gut microbiota drives macrophage-dependent self-renewal of intestinal stem cells via niche enteric serotonergic neurons显示文摘Lgr5+intestinal stem cells(ISCs)reside within specialized niches at the crypt base and harbor self-renewal and differentiation capacities.ISCs in the crypt base are sustained by their surrounding niche for precise modulation of self-renewal and differentiation.However,how intestinal cells in the crypt niche and microbiota in enteric cavity coordinately regulate ISC stemness remains unclear.Here,we show that ISCs are regulated by microbiota and niche enteric serotonergic neurons.The gut microbiota metabolite valeric acid promotes Tph2 expression in enteric serotonergic neurons via blocking the recruitment of the NuRD complex onto Tph2 promoter.5-hydroxytryptamine(5-HT)in turn activates PGE2 production in a PGE2+macrophage subset through its receptors HTR2A/3 A;and PGE2 via binding its receptors EP1/EP4,promotes Wnt/β-catenin signaling in ISCs to promote their self-renewal.Our findings illustrate a complex crosstalk among microbiota,intestinal nerve cells,intestinal immune cells and ISCs,revealing a new layer of ISC regulation by niche cells and microbiota. | Pingping Zhu Tiankun Lu Jiayi Wu Dongdong Fan Benyu Liu Xiaoxiao Zhu Hui Guo Ying Du Feng Liu Yong Tian Zusen Fan | 2022 | Cell Research2022，32，6： | 8 |
| 3 | Identification of Maximum Road Friction Coefficient and Optimal Slip Ratio Based on Road Type Recognition显示文摘The identification of maximum road friction coefficient and optimal slip ratio is crucial to vehicle dynamics and control.However,it is always not easy to identify the maximum road friction coefficient with high robustness and good adaptability to various vehicle operating conditions.The existing investigations on robust identification of maximum road friction coefficient are unsatisfactory.In this paper,an identification approach based on road type recognition is proposed for the robust identification of maximum road friction coefficient and optimal slip ratio.The instantaneous road friction coefficient is estimated through the recursive least square with a forgetting factor method based on the single wheel model,and the estimated road friction coefficient and slip ratio are grouped in a set of samples in a small time interval before the current time,which are updated with time progressing.The current road type is recognized by comparing the samples of the estimated road friction coefficient with the standard road friction coefficient of each typical road,and the minimum statistical error is used as the recognition principle to improve identification robustness.Once the road type is recognized,the maximum road friction coefficient and optimal slip ratio are determined.The numerical simulation tests are conducted on two typical road friction conditions(single-friction and joint-friction)by using CarSim software.The test results show that there is little identification error between the identified maximum road friction coefficient and the pre-set value in CarSim.The proposed identification method has good robustness performance to external disturbances and good adaptability to various vehicle operating conditions and road variations,and the identification results can be used for the adjustment of vehicle active safety control strategies. | GUAN Hsin WANG Bo LU Pingping XU Liang | 2014 | Chinese Journal of Mechanical Engineering2014，27，5： | 8 |
| 4 | Parkin promotes proteasomal degradation of p62: implication of selective vulnerability of neuronal cells in the pathogenesis of Parkinson's disease显示文摘 | Pingping Song Shanshan Li Hao Wu Ruize Gao Guanhua Rao Dongmei Wang Ziheng Chen Biao Ma Hongxia Wang Nan Sui Haiteng Deng Zhuohua Zhang Tieshan Tang Zheng Tan Zehan Han Tieyuan Lu Yushan Zhu Quan Chen | 2016 | Protein & Cell2016，7，2： | 8 |
| 5 | Multichannel DEM reconstruction method based on Markov random fields for bistatic SAR显示文摘It appears very interesting and particularly useful to perform DEM reconstruction with the configuration of multichannel bistatic SAR(Bi SAR) because of its considerable advantages. In typical processing flow of multichannel interferometric synthetic aperture radar(In SAR), techniques based on statistical methods or iterative methods does not work very well with limits of specific Bi SAR configurations. Moreover, the tradeoff between the correctness and precision of the reconstruction of different baselines needs to be overcome as well.Based on the advantages of multichannel Bi SAR configuration, basic idea is the joint utilization of amplitude data, iterative reconstruction results and ML results to help the optimization of the total MRF energy function.Thus, a multichannel DEM reconstruction method based on Markov Random Fields(MRFs) for Bi SAR, which concerns much about discontinuities and energy functions, is proposed to achieve not only correct but also more precise results. Afterward, the strategy to determine the weight parameter and processing flow is presented.Finally, simulated and real data experiments are given to validate the proposed method. | HONG Feng TANG JiangWen LU PingPing | 2015 | Science China(Information Sciences)2015，58，6： | 5 |
| 6 | Structure and quality controlled growth of InAs nanowires through catalyst engineering显示文摘在这研究,结构和用在一个分子的横梁取向附生反应堆的 Au 催化剂的 InAs nanowires 的 quality controlled 生长被介绍。由在催化剂调节铟集中,没有缺点的 wurtzite 结构和没有缺点的锌闪锌矿结构 InAs nanowires 能被导致。这些没有缺点的锌闪锌矿结构 InAs nanowires 沿着\成长,这被发现( \left\langle { \bar 1 \bar 10 } \right\rangle \)方向与四低精力{ 111 }并且二{ 110 }方面墙方面并且采用\( \left ({ \bar 1 \bar 1 \bar 1 } \right )\) catalyst/nanowire 接口。我们的结构、化学的描述和计算为 InAs nanowire 生长识别催化剂过度饱和阀值的存在。什么时候在里面在催化剂的集中足够地高,没有缺点的锌闪锌矿结构 InAs nanowires 能被导致。这研究通过催化剂工程提供卓见进晶体结构的操作和 IIIV 半导体 nanowires 的结构质量。 | ZhiZhang Zhenyu Lu Hongyi Xu Pingping Chen Wei Lu Jin Zou | 2014 | Nano Research2014，7，11： | 4 |
| 7 | Recent advances in pharmaceuticals and personal care products in the surface water and sediments in China显示文摘 | Wentao Zhao Ying Guo Shuguang Lu Pingping Yan Qian Sui | 2016 | Frontiers of Environmental Science & Engineering2016，10，6： | 4 |
| 8 | The Arabidopsis catalase triple mutant reveals important roles of catalases and peroxisome-derived signaling in plant development显示文摘Hydrogen peroxide(H_2O_2)is generated in many metabolic processes.As a signaling molecule,H_2O_2plays important roles in plant growth and development,as well as environmental stress response.In Arabidopsis,there are three catalase genes,CAT1,CAT2,and CAT3.The encoded catalases are predominately peroxisomal proteins and are critical for scavenging H_2O_2.Since CAT1 and CAT3 are linked on chromosome 1,it has been almost impossible to generate cat1/3 and cat1/2/3 mutants by traditional genetic tools.In this study,we constructed cat1/3 double mutants and cat1/2/3 triple mutants by CRISPR/Cas9 to investigate the role of catalases.The cat1/2/3 triple mutants displayed severe redox disturbance and growth defects under physiological conditions compared with wild-type and the cat2/3 double mutants.Transcriptome analysis showed a more profound transcriptional response in the cat1/2/3 triple mutants compared to the cat2/3 mutants.These differentially expressed genes are involved in plant growth regulation as well as abiotic and biotic stress responses.In addition,expression of OXI1(OXIDATIVE SIGNAL INDUCIBLE 1)and several MAPK cascade genes were changed dramatically in the catalase triple mutant,suggesting that H_2O_2produced in peroxisomes could serve as a peroxisomal retrograde signal. | Tong Su Pingping Wang Huijuan Li Yiwu Zhao Yao Lu Peng Dai Tianqi Ren Xiaofeng Wang Xuezhi Li Qun Shao Dazhong Zhao Yanxiu Zhao Changle Ma | 2018 | Journal of Integrative Plant Biology2018，60，7： | 3 |
| 9 | Biodegradation of bezafibrate by the activated sludge under aerobic condition:Effect of initial concentration,temperature and pH显示文摘Lab-scale experiments were conducted to investigate the effect of initial concentration,temperature and pH on the removal of bezafibrate(BF)by activated sludge under aerobic condition.The results showed that adsorption of BF onto activated sludge was negligible,and biodegradation was the main removal mechanism of BF.The removal of BF in the aqueous phase by the activated sludge can be described by a pseudo-first-order reaction.The reaction rate constants had a negative relationship with the initial concentration of BF,and dramatically reduced from 0.050 to 0.007 h^-1,when the temperature dropped from 20℃to 10℃.Variation of pH between 5.0 and 9.0 did not have significant influence on the removal of BF,indicating a high adaptation of microorganism in the activated sludge responsible for BF degradation to a wide pH range.The findings of this study are helpful to improve the removal of pharmaceuticals during the wastewater treatment plants by selecting the appropriate process variables,and eventually eliminate their release to the environment. | Qian Sui Pingping Yan Xuqi Cao Shuguang Lu Wentao Zhao Maozhe Chen | 2016 | Emerging Contaminants2016，2，4： | 2 |
| 10 | Preparation and characterization of graphite nanosheets from ultrasonic powdering technique显示文摘 | Guohua Chen Wengui Weng Dajun Wu Cuiling Wu Jinrong Lu Pingping Wang Xiangfeng Chen | 2004 | Carbon2004，，4： | 2 |
| 11 | Au-catalysed free-standing wurtzite structured InAs nanosheets grown by molecular beam epitaxy显示文摘In this study,we report the growth of free-standing InAs nanosheets using Au catalysts in molecular beam epitaxy.Detailed structural characterizations suggest that wurtzite structured InAs nanosheets,with features of extensive{1120}surfaces,grown along the<1102>direction and adopted{0001}nanosheet/catalyst interfaces,are initiated from wurtzite structured[0001]nanowires as the inclined epitaxial growth due to relatively higher In concentrations in Au catalysts,and grown from these inclined nanostructures through catalyst-induced axial growth and their enhanced lateral growth under the high growth temperature.Based on the facts that the nanosheets contain large low energy{1120}surfaces and{0001}nanosheet/catalyst interfaces,the growth of our nanosheets is a thermodynamically driven process.This study provides new insights into fabricating free-standing Ⅲ-Ⅴ nanosheets for their applications in future nanoscale devices. | Qiang Sun Han Gao Xiaomei Yao Kun Zheng Pingping Chen Wei Lu Jin Zou | 2019 | Nano Research2019，12，11： | 2 |
| 12 | Responses of electrical properties of tea leaves to low-temperature stress显示文摘Critical temperature is one of the most important parameters for the control of crop frost protection through airflow disturbance.It changes with complex weather conditions,thus it is difficult to be determined.A method of testing electrical property of tea leaves under cold stress was put forward to indicate critical temperature.The testing system was established to measure the capacitance,impedance,resistance and reactance of the samples under different air temperatures,air humidities and airflow velocities.The variation of the electrical property was also analyzed.The results show that at humidity below 70%and airflow velocity of 0 m/s the impedance and resistance increased slowly,while the reactance kept steady when air temperature decreased from 8.0°C to around−6.3°C,and then increased rapidly from around−6.3°C to−15.0°C.There were no significant differences of the above parameters and variation trend under different airflow velocities.There was an exponential relationship between the impedance and the temperature.The capacitance was rather small and almost no change occurred with air temperature under different conditions of air velocity and humidity,except a few abrupt peaks.The maximum peak capacitance was representative of its response at certain humidity and airflow velocity.The typical temperatures were close to a range,where the other three parameters began to increase rapidly.The typical temperature dropped to the lowest of−7.8°C at the airflow velocity of 0 m/s.Therefore,the characteristic response of the capacitance could indicate critical temperature of tea leaves. | Lu Yongzong Hu Yongguang Zhang Xiliang Li Pingping | 2015 | International Journal of Agricultural and Biological Engineering2015，8，5： | 2 |
| 13 | Novel Evaluation Method of Vehicle Suspension Performance Based on Concept of Wheel Turn Center显示文摘The current research of suspension performance evaluation is mixed in the evaluation of vehicle handling and ride comfort. However, it is lack of a direct and independent evaluation method for suspension performance. In this paper, a novel wheel turn center method is proposed to evaluate the suspension performance. This method is based on the concept and application of wheel turn center(WTC) and sprung mass turn center(SPTC). The vehicle body and each wheel are regarded to be independent rigid bodies and have their own turn centers which reflect respective steering motions and responses. Since the suspension is the link between vehicle body and wheels, the consistence between the sprung mass turn center and the wheel turn center reflects the effect and performance of the suspension system. Firstly, the concept and appropriate calculation method of WTC and SPTC are developed. Then the degree of inconsistence between WTC and SPTC and the time that they achieve consistence, when the vehicle experiences from transient steering to steady steering state, are proposed to evaluate suspension performance. The suspension evaluation tests are conducted under different vehicle velocities and lateral accelerations by using Car Sim software. The simulation results show that the inconsistence of steering motion between vehicle body and wheels are mainly at high speeds and low lateral accelerations. Finally, based on the proposed evaluation indexes, the influences of different suspension characteristic parameters on suspension performance and their matches to improve steering coordination are discussed. The proposed wheel turn center method provides a guidance and potential application for suspension evaluation and optimization. | WANG Bo GUAN Hsin LU Pingping ZHAN Jun | 2015 | Chinese Journal of Mechanical Engineering2015，28，5： | 2 |
| 14 | Circular RNA circZbtb20 maintains ILC3 homeostasis and function via Alkbh5-dependent m6A demethylation of Nr4a1 mRNA显示文摘Group 3 innate lymphoid cells(ILC3s)play critical roles in innate immunity and gut homeostasis.However,how ILC3 homeostasis is regulated remains elusive.Here,we identified a novel circular RNA,circZbtb20,that is highly expressed in ILC3s and required for their maintenance and function.CircZbtb20 deletion causes reduced ILC3 numbers,increasing susceptibility to C.rodentium infection.Mechanistically,circZbtb20 enhances the interaction of Alkbh5 with Nr4a1 mRNA,leading to ablation of the m6A modification of Nr4a1 mRNA to promote its stability.Nr4a1 initiates Notch2 signaling activation,which contributes to the maintenance of ILC3 homeostasis.Deletion of Alkbh5 or Nr4a1 also impairs ILC3 homeostasis and increases susceptibilities to bacterial infection.Thus,our findings reveal an important role of circular RNA in the regulation of innate lymphoid cell homeostasis. | Benyu Liu Nian Liu Xiaoxiao Zhu Liuliu Yang Buqing Ye Huimu Li Pingping Zhu Tiankun Lu Yong Tian Zusen Fan | 2021 | Cellular & Molecular Immunology2021，18，6： | 2 |
| 15 | Comprehensive identification of lysine 2-hydroxyisobutyrylated proteins in Ustilaginoidea virens reveals the involvement of lysine 2-hydroxyisobutyrylation in fungal virulence显示文摘Lysine 2-hydroxyisobutyrylation(Khib) is a newly identified post-translational modification(PTM) that plays important roles in transcription and cell proliferation in eukaryotes. However, its function remains unknown in phytopathogenic fungi. Here,we performed a comprehensive assessment of Khibin the rice false smut fungus Ustilaginoidea virens, using Tandem Mass Tag(TMT)-based quantitative proteomics approach. A total of 3 426 Khibsites were identified in 977 proteins, sugg esting that Khibis a common and complex PTM in U. virens. Our data demonstrated that the2-hydroxyisobutyrylated proteins are involved in diverse biological processes. Network analysis of the modified proteins revealed a highly interconnected protein network that included many well-studied virulence factors. We confirmed that the Zn-binding reduced potassium dependency3-type histone deacetylase(UvRpd3) is a major enzyme that removes 2-hydroxyisobutyrylation and acetylation in U. virens. Notably, mutations of Khib sites in the mitogen-activated protein kinase(MAPK)UvSlt2 significantly reduced fungal virulence and decreased the enzymatic activity of UvSlt2. Molecular dynamics simulations demonstrated that 2-hydroxyisobutyrylation in UvSlt2 increased the hydrophobic solvent-accessible surface area and thereby affected binding between the UvSlt2 enzyme and its substrates. Our findings thus establish Khibas a major post-translational modification in U. virens and point to an important role for Khibin the virulence of this phytopathogenic fungus. | Xiaoyang Chen Xiabing Li Pingping Li Xiaolin Chen Hao Liu Junbin Huang Chaoxi Luo Tom Hsiang Lu Zheng | 2021 | Journal of Integrative Plant Biology2021，63，2： | 2 |
| 16 | Research on Key Issues of Consistency Analysis of Vehicle Steering Characteristics显示文摘Given the global lack of effective analysis methods for the impact of design parameter tolerance on performance deviation in the vehicle proof-of-concept stage,it is difficult to decompose performance tolerance to design parameter tolerance.This study proposes a set of consistency analysis methods for vehicle steering performance.The process of consistency analysis and control of automotive performance in the conceptual design phase is proposed for the first time.A vehicle dynamics model is constructed,and the multi-objective optimization software Isight is used to optimize the steering performance of the car.Sensitivity analysis is used to optimize the design performance value.The tolerance interval of the performance is obtained by comparing the original car performance value with the optimized value.With the help of layer-by-layer decomposition theory and interval mathematics,automotive performance tolerance has been decomposed into design parameter tolerance.Through simulation and real vehicle experiments,the validity of the consistency analysis and control method presented in this paper are verified.The decomposition from parameter tolerance to performance tolerance can be achieved at the conceptual design stage. | Yanhua Liu Xin Guan Pingping Lu Rui Guo | 2021 | Chinese Journal of Mechanical Engineering2021，34，2： | 2 |
| 17 | Peroxisome proliferator‐activated receptor‐γ activation inhibits hepatocellular carcinoma cell invasion by upregulating plasminogen activator inhibitor‐1显示文摘 | Xiaojuan Pang Yinna Wei Ya Zhang Minyue Zhang Yan Lu Pingping Shen | 2013 | Cancer Sci2013，，6： | 1 |
| 18 | Effect of heat treatment on the structure and properties of poly(2,6-benzothiazole) (ABPBT) and poly (2,5-benzoxazole) (ABPBO)显示文摘 | Wang Shan-feng Bao Gao-bin Lu Zhi-bao Wu Pingping Han Zhe-wen | 2000 | Journal of Materials Science2000，35，： | 1 |
| 19 | Preparation properties of a γ-Al2O3 washcoat deposited on a ceramic honeycomb显示文摘 | Jiang Pingping Lu Guanzhong Guo Yanglong | 2005 | Surface & Coatings Technology2005，190，23： | 1 |
| 20 | Simulation Research on Dynamic Characteristics of Hydraulic Mount显示文摘At present,research on hydraulic mounts has mainly focused on the prediction of the dynamic stiffness and loss angle.Compared to the traditional finite element analysis method,the programming method can be used to analyze hydraulic mounts for a rapid and accurate understanding of the influence of the different mounting parameters on the dynamic stiffness and loss angle.The aims of this study were to investigate the nonlinear dynamic characteristics of a hydraulic mount,and to identify the parameters that affect the dynamic stiffness and loss angle using MATLAB software programs to obtain the influence curves of the parameters,so as to use suitable parameters as the basis for vibration analysis.A nonlinear mechanical model of a hydraulic mount was established according to the basic principles of fluid dynamics.The dynamic stiffness and loss angle of the dimensionless expression were proposed.A numerical calculation method for the dynamic performance evaluation index of the hydraulic mount was derived.A one-to-one correspondence was established between the structural parameters and peak frequency of the evaluation index.The accuracy and applicability of the mechanical model were verified by the test results.The results demonstrated the accuracy of the nonlinear mechanical model of the hydraulic mount,and the vehicle driving comfort was greatly improved by the optimization of the structural parameters. | Yanhua Liu Xin Guan Pingping Lu Rui Guo | 2021 | Chinese Journal of Mechanical Engineering2021，34，3： | 1 |
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