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| 1 | Comprehensive identification of lysine 2-hydroxyisobutyrylated proteins in Ustilaginoidea virens reveals the involvement of lysine 2-hydroxyisobutyrylation in fungal virulence显示文摘Lysine 2-hydroxyisobutyrylation(Khib) is a newly identified post-translational modification(PTM) that plays important roles in transcription and cell proliferation in eukaryotes. However, its function remains unknown in phytopathogenic fungi. Here,we performed a comprehensive assessment of Khibin the rice false smut fungus Ustilaginoidea virens, using Tandem Mass Tag(TMT)-based quantitative proteomics approach. A total of 3 426 Khibsites were identified in 977 proteins, sugg esting that Khibis a common and complex PTM in U. virens. Our data demonstrated that the2-hydroxyisobutyrylated proteins are involved in diverse biological processes. Network analysis of the modified proteins revealed a highly interconnected protein network that included many well-studied virulence factors. We confirmed that the Zn-binding reduced potassium dependency3-type histone deacetylase(UvRpd3) is a major enzyme that removes 2-hydroxyisobutyrylation and acetylation in U. virens. Notably, mutations of Khib sites in the mitogen-activated protein kinase(MAPK)UvSlt2 significantly reduced fungal virulence and decreased the enzymatic activity of UvSlt2. Molecular dynamics simulations demonstrated that 2-hydroxyisobutyrylation in UvSlt2 increased the hydrophobic solvent-accessible surface area and thereby affected binding between the UvSlt2 enzyme and its substrates. Our findings thus establish Khibas a major post-translational modification in U. virens and point to an important role for Khibin the virulence of this phytopathogenic fungus. | Xiaoyang Chen Xiabing Li Pingping Li Xiaolin Chen Hao Liu Junbin Huang Chaoxi Luo Tom Hsiang Lu Zheng | 2021 | Journal of Integrative Plant Biology2021，63，2： | 2 |
| 2 | Polyetheretherketone with citrate potentiated influx of copper boosts osteogenesis,angiogenesis,and bacteria-triggered antibacterial abilities显示文摘A well designed coating for polyetheretherketone(PEEK)implants can provide enough support to overcome crucial medical challenges,which are insufficient osseointegration and high rate of infection.Herein,we utilize the co-deposition of polydopamine(PDA)and copper-citrate nanoclusters to construct a p H-responsive coating on porous PEEK for synergistic bone regeneration,vascular formation and anti-infection.Specifically,this PDA coating released high dose of copper and citrate at lower p H value,which increased intracellular copper content,boosted production of reactive oxygen species and severe damage of protein,leading to killing of 93%planktonic bacterial and eradication of adherent bacteria.At p H of 7.4,the release of copper and citrate were in a slow and sustained behavior,synergistically enhanced vascular formation potential and osteodiffereration of Ad-MSC in vitro.After implanted in rabbit tibia for 6 and 12 weeks,the micro-CT evaluation and histological analysis consistently highlighted the ability of this PDA coating to increase new bone formation adjacent to coated PEEK implant and enhance bone-implant interfacial integration.These results were proven to be related to the synergistic effect that citrate facilitated a 2-fold influx of copper into cells,which not only enhanced the bacteria-killing ability but also encouraged bone regeneration of implants.This present work provides an effective method to control infections while promoting osseointegration simultaneously,which will show tremendous clinical application and can be a solution to current challenges facing orthopedics. | Jianglong Yan Dandan Xiab Pan Xionga Yangyang Li Wenhao Zhou Qiyao Li Pei Wang Yufeng Zheng Yan Cheng | 2021 | Journal of Materials Science & Technology2021，，12： | 1 |
| 3 | China and South Korea FTA Will Bring New Opportunities显示文摘On February 25,2015,China and South Korea have initialed their free trade agreement(FTA),the end of the FTA negotiations,according to a brief statement on the Ministry of Commerce(MOC)website.The China-South Korea FTA is a high-level and comprehensive one that also includes investment and services trade.What’s in the FTA?According to the information | Li Xiabing | 2015 | China's Foreign Trade2015，，2： | 0 |
| 4 | Sentiment classification via user and product interactive modeling显示文摘Sentiment classification aims to identify the polarity of a given review. Most existing methods consider each review as an individual while ignoring the importance of the user and product information of the given review. A direct way to integrate user and product information is to employ an attention mechanism to learn the local interaction between them. However, local interactions cannot capture the global optimization among user and product information. Therefore, we propose a novel interactive model to integrate both local and global interactions between users and products. In particular, we employ an attention mechanism to learn local interactions between users and products, and construct user and product interactive graphs to model the global interaction of users and products. Empirical evaluation shows that our model outperforms previous state-of-the-art methods significantly by learning the local and global interactions among users’ preferences, product characteristics, and reviews. | Xiabing ZHOU Zhongqing WANG Min ZHOU Qifa WANG Shoushan LI Min ZHANG Guodong ZHOU | 2021 | Science China(Information Sciences)2021，64，12： | 0 |
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