|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Preparation and activity of conjugate of monoclonal antibody HAb18 against hepatoma F( ab′ )_2 fragment and staphylococcal enterotoxin A显示文摘AIM To prepare the conjugate of staphylococcal enterotoxin A (SEA) protein which is a bacterial SAg and the F(ab')2 fragment of mAb HAbl8 against human hepatocellular carcinoma (HCC), and identify its activity in order to use SAg in the targeting therapy of HCC.METHODS MAb HAbl8 was extracted from the abdominal dropsy of Balb/ c mice, and was purified through chromatography column SP-40HR with Fast protein liquid chromatography (FPLC) system. The F(ab')2 fragment of mAb HAb18 was prepared by papainic digestion method. The conjugate of mAb HAb18 F(ab')2fragment and SEA was prepared with chemical conjugating reagent N-succinimidyl-3-( 2-pyridyldithio) propionate (SPDP) and purified through chromatography column Superose 12with FPLC system. The molecular mass and purity of each collected peak were identified with SDS-PAGE assay. The protein content was assayed by Lowry's method. The antibody activity of HAb18 F (ab')2 against HCC in the conjugate was identified by indirect immunocytochemical ABC method, and the activity of SEA in the conjugate to activate peripheral blood mononuclear cells (PBMC) was identified with MTT assay.RESULTS The lgG mAb HAb18 was extracted,and purified successfully. Immunocytochemical staining demonstrated that it reacted with most of HHCC cells of human HCC cell line. There were two peaks in the process of purification of the prepared HAb18 F(ab)2-SEA conjugate. SDS-PAGE assay demonstrated that the molecular mass of the first peak was about 130 ku, and the second peak was the mixture of about 45 ku and a little 100 ku proteins. The immunocytochemical staining was similar in HAb18 F (ab ')2-SEAconjugate and HAb18 F (ab ')2, i.e., thecytoplasm and/or cell membranes of most HHCC cells were positively stained. The MTT assay showed that the optical absorbance (A) value at 490 nm of HAb18 F (ab')2-SEA conjugate was 0.182 ± 0.012, that of negative control was 0.033± 0.009, and there was significant difference between them ( P < 0.05).CONCLUSION SPDP is a good proteinconjugating reagent and can be used in preparing protein conjugate. The conjugate of mAb HAb18F(ab')2 fragment and SEA protein was preparedsuccessfully in present study and can be used in the experimental study of HCC targeting therapy with the conjugate of SAg and anti-HCC mAbs or their fragments. | Lian Jun Yang Yan Fang Sui Zhi Nan Chen Department of Pathology, Fourth Military Medical University. Xi’an 710032, Shaanxi Province, China | 2001 | World Journal of Gastroenterology2001，7，2： | 20 |
| 2 | Reactive oxygen species-based nanomaterials for the treatment of myocardial ischemia reperfusion injuries显示文摘Interventional coronary reperfusion strategies are widely adopted to treat acute myocardial infarction,but morbidity and mortality of acute myocardial infarction are still high.Reperfusion injuries are inevitable due to the generation of reactive oxygen species(ROS)and apoptosis of cardiac muscle cells.However,many antioxidant and anti-inflammatory drugs are largely limited by pharmacokinetics and route of administration,such as short half-life,low stability,low bioavailability,and side effects for treatment myocardial ischemia reperfusion injury.Therefore,it is necessary to develop effective drugs and technologies to address this issue.Fortunately,nanotherapies have demonstrated great opportunities for treating myocardial ischemia reperfusion injury.Compared with traditional drugs,nanodrugs can effectively increase the therapeutic effect and reduces side effects by improving pharmacokinetic and pharmacodynamic properties due to nanodrugs’size,shape,and material characteristics.In this review,the biology of ROS and molecular mechanisms of myocardial ischemia reperfusion injury are discussed.Furthermore,we summarized the applications of ROS-based nanoparticles,highlighting the latest achievements of nanotechnology researches for the treatment of myocardial ischemia reperfusion injury. | Tianjiao Zhao Wei Wu Lihua Sui Qiong Huang Yayun Nan Jianhua Liu Kelong Ai | 2022 | Bioactive Materials2022，7，1： | 11 |
| 3 | Parkin promotes proteasomal degradation of p62: implication of selective vulnerability of neuronal cells in the pathogenesis of Parkinson's disease显示文摘 | Pingping Song Shanshan Li Hao Wu Ruize Gao Guanhua Rao Dongmei Wang Ziheng Chen Biao Ma Hongxia Wang Nan Sui Haiteng Deng Zhuohua Zhang Tieshan Tang Zheng Tan Zehan Han Tieyuan Lu Yushan Zhu Quan Chen | 2016 | Protein & Cell2016，7，2： | 8 |
| 4 | Effects of flooding stress on the photosynthetic apparatus of leaves of two Physocarpus cultivars显示文摘We applied under pot-culture conditions and the double-casing pot method to study the characteristics of photosynthetic gas exchange and chlorophyll fluorescence in the leaves of Physocarpus amurensis Maxim(PA) and Physocarpus opulifolius under flooding stress.Our results indicate a significantly higher flooding tolerance of P.opulifolius compared to P.amurensis.Especially in P.amurensis, the limitation of non-stomatal factors played a major role in the advanced stages of flooding stress,observed as a rapid increase of the intercellular CO_2 concentration(C_i) and a decrease of the stomatal limitation value(L_s).The maximal PSII photochemical efficiencies(F_v/F_m) and actual photochemical efficiency(?_(PSII)) in the leaves of P.opulifolius were significantly higher, and the extent of decrease during the flooding process was smaller than in P.amurensis.In addition, the non-chemical quenching(NPQ) in the leaves of P.opulifolius significantly increased from the 10 th day under flooding stress,while the variation of NPQ in the leaves of P.amurensis was much smaller.This indicates that the leaves of P.opulifolius had not only higher PSII photochemical activity, but also improved tolerance to flooding stress, which may be caused by its ability to dissipate excess excitation energy by starting NPQ.At the 16 th day under flooding stress, the PIABSsignificantly decreased with greater extent of decrease than F_v/F_m in the leaves of both Physocarpus,but the decreasing extent of PIABSin P.opulifolius was significantly smaller than in P.amurensis.In the 16 th day under flooding stress, the fluorescence at J and I point(V_J and V_I) in P.amurensis were significantly higher, and the extent of increase in V_J was greater than V_I.However, the variations of V_Jand V_I in the leaves of P.opulifolius were smaller, suggesting that the damage sites of flooding stress to PSII in the leaves of P.amurensis were mainly located in the electron transport process from Q_A at the PSII receptor side to Q_B.Flooding stress reduced the proportion(φ Eo) of luminous energy absorbed by the PSII reaction center for the electron transport following Q_A^-, while the maximum quantum yield(φ Do) of non-photochemical quenching increased.However, the TR_o/RC and ETo/RC in the leaves of P.amurensis decreased accompanied by a dramatic increase of energy(DI_o/RC) from the dissipation in the reaction center.This further indicated that the function of the PSII reaction center in the leaves of P.amurensis was significantly lower than in P.opulifolius. | Huihui Zhang Peng Feng Wei Yang Xin Sui Xin Li Wei Li Rongtao Zhang Siyu Gu Nan Xu | 2018 | Journal of Forestry Research2018，29，4： | 6 |
| 5 | Interaction between γ-TiAl alloy and zirconia显示文摘A study on the interaction between TiAl alloy and zirconia was carried out in argon atmosphere. Themicrographic observations of the cross-section perpendicular to the interface were obtained using a scanning electronmicroscope with a dedicated energy dispersive spectrometer (SEM/EDS). The results showed that firstly TiAl alloyspreads on the ceramic surface, and then infiltrates into the pores between ceramic particles accompanied by a chemicalreaction. The whole ceramic mold is broken into tiny blocks. A multiple fission reaction mechanism was developed toexplain the interaction between TiAl alloy and zirconia. | Liu Aihui Li Bangsheng Nan Hai Sui Yanwei Guo Jingjie Fu Hengzhi | 2008 | China Foundry2008，5，1： | 6 |
| 6 | Extremely high-rate aqueous supercapacitor fabricated using doped carbon nanoflakes with large surface area and mesopores at near-commercial mass loading显示文摘在基于所有碳电极并且在良性的水的媒介操作而不是常规器官的电解质的 supercapacitor 完成令人满意的精力力量联合是主要挑战。为这个目的,我们制作了碳 nanoflakes (在厚度的 20-100 nm,在宽度的 5-m ) 包含一个大表面区域的无匹的联合(3,000 m 2sible 光活动被发现是 29.23 和 58.82 摩尔 ?? 挠潲獳氭湩敫 ? 牧灡敨敮 ?????? ?? 吗?? | Nan Mao Huanlei Wang Yang Sui Yongpeng Cui Jesse Pokrzywinski Jing Shi Wei Liu Shougang Chen Xin Wang David Mitlin | 2017 | Nano Research2017，10，5： | 5 |
| 7 | Enhancement of Aggression Induced by Isolation Rearing is Associated with a Lack of Central Serotonin显示文摘Isolation rearing(IR) enhances aggressive behavior, and the central serotonin(5-hydroxytryptamine,5-HT) system has been linked to IR-induced aggression.However, whether the alteration of central serotonin is the cause or consequence of enhanced aggression is still unknown. In the present study, using mice deficient in central serotonin Tph2-/-and Lmx1 b-/-, we examined the association between central serotonin and aggression with or without social isolation. We demonstrated that central serotonergic neurons are critical for the enhanced aggression after IR. 5-HT depletion in wild-type mice increased aggression. On the other hand, application of 5-HT in Lmx1 b-/-mice inhibited the enhancement of aggression under social isolation conditions. Dopamine was downregulated in Lmx1 b-/-mice. Similar to 5-HT, L-DOPA decreased aggression in Lmx1 b-/-mice. Our results linkthe serotoninergic system directly to aggression and this may have clinical implications for aggression-related human conditions. | Yiqiong Liu Yunong Sun Xiaoyan Zhao Ji-Young Kim Lu Luo Qian Wang Xiaolu Meng Yonghui Li Nan Sui Zhou-Feng Chen Chuxiong Pan Liang Li Yan Zhang | 2019 | Neuroscience Bulletin2019，35，5： | 5 |
| 8 | Selenoprotein P mRNA expression in human hepatic tissues显示文摘AIM: To investigate the expression of selenoprotein P mRNA (SePmRNA) in tissues of normal liver, liver cirrhosis and hepatocellular carcinoma (HCC), and its relationship with HCC occurrence and development. METHODS: The expression of SePmRNA in tissues of normal liver, liver cirrhosis and HCC were detected by in situ hybridization using a cDNA probe. RESULTS: The enzyme digesting products of PBluescript-H uman Selenoprotein P were evaluated by electrophoresis. The positive expression of SePmRNA was found in the tissues of normal liver, liver cirrhosis and HCC. The expression of SeP mRNA was found in hepatic interstitial substance, especially in endothelial cells and lymphocytes of vasculature. The positive rate of SePmRNA in normal liver tissue was 84.6% (11/13) and the positive signals appeared in the nucleus and cytoplasm, mostly in the nucleolus, and the staining granules were larger in the nucleolus and around the nucleus. The positive rate of SePmRNA in liver cirrhosis tissue was 45.0% (9/20) and the positive signals were mainly in the nucleolus and cytoplasm, being less around the nucleus and inner nucleus than that in normal liver tissue. The positive rate of SePmRNA in HCC tissue was 30.0% (9/30) and the positive signals were in the cytoplasm, but less in the nucleus. CONCLUSION: SePmRNA expression in the tissues of normal liver and HCC is significantly different (84.6% vs 30.0%, P = 0.003), suggesting that SeP might play a role in the occurrence and development of HCC. | Chun-Li Li Ke-Jun Nan Tao Tian Chen-Guang Sui Yan-Fang Liu | 2007 | World Journal of Gastroenterology2007，13，16： | 4 |
| 9 | Burning products of TA15 titanium alloy by friction oxygen concentration method显示文摘The microstructural characteristics, elemental distribution law and microscopic formation mechanism of the burning products of TA15 titanium alloy were investigated by friction oxygen concentration method, associated with in situ observation, X-ray diffraction(XRD),scanning electron microscopy(SEM) and energy-dispersive spectroscopy(EDS) analyses, providing the thoughts to improve fireproof property. The results show that, when the friction contact pressure(p_(fric)) is 0.20 MPa and oxygen concentration of premixed air flow(c_0) is 60 vol%, TA15 titanium alloy produces violent sparks and presents dazzling white light during combustion. The generated products after burning are mainly TiO_2 and small amount of Al_2O_3 oxides. Four distinct zones form from the combustion surface to the alloy matrix, and they are in the sequence of combustion zone, fusion zone, heat-affected zone and transition zone. Further, combustion zone is composed of TiO_2 and A1203 compounds, containing obvious cracks. In the fusion zone, discontinuous oxygenrich Al-based solid solution forms, and the elemental distribution has strong volatility. In the heat-affected zone,there are abundant of Ti-based solid solution and small amount of Al-and Mo-based solid solution. Transition zone is made of lamellar structure. Two technical approaches are given to prevent oxygen diffusion inside the reaction zone and reaction-affected zone. On the one hand,the content of Al is designed as the upper limit of alloy composition; on the other hand, fireproof coatings are deposited on the surface of the alloy. | Nan Sui Guang-Bao Mi Meng-Qi Yan Jing-Xia Cao Xu Huang | 2018 | Rare Metals2018，37，11： | 1 |
| 10 | Hupo powder promotes autophagy of menstrual blood-derived stem cells from patients with endometriosis显示文摘Objective:To explore the effect of Hupo powder(HP)on autophagy in menstrual blood-derived stem cells(MenSCs)with endometriosis(EMT).Methods:EMT MenSCs(E-MenSCs)and healthy MenSCs(H-MenSCs)were isolated from the menstrual blood of patients with EMT and healthy female participants,respectively.We identified their stem cells’characteristics via adipogenic and osteogenic differentiation.Twelve male SpragueeDawley rats received 0.9% NaCl and HP-dispensing granules by gastric irrigation to prepare blank serum and medicated serum,respectively.We used serum concentrations of 5%,10%,and 20%,each at administered times of 12,24,and 48 h to select the best condition.These cells were divided into three groups:blank serum of the control group,blank serum of the model group,and medicated serum of the HP group.H-MenSCs were used in the control group,while E-MenSCs were used in the model and HP groups.We analyzed cell viability using a cell counting kit-8 assay,observed cell morphology,evaluated the amounts of auto-phagosomes and autolysosomes by transmission electron microscopy,and detected the protein expression of autophagy markers(LC3-II and Beclin1)by Western blot.Results:E-MenSCs and H-MenSCs became long fusiform with a diffuse radial pattern,forming lipid droplets and calcium nodules after adipogenic and osteogenic differentiation.We then used the best conditiond 20% serum and 48 hdfor the subsequent experiments.In contrast to the model group,the HP group exhibited lower cell viability(=0.007),larger amounts of autophagosomes and autolysosomes(P<0.001 and P=0.001,respectively),and higher expression of LC3-II and Beclin1(P=0.021 and P=0.019,respectively).Conclusion:Hupo powder can promote autophagy in E-MenSCs,which might be one of the mechanisms underlying its therapeutic effects. | Yuejian Zhang Changxiang Li Conglu Sui Xiuping Zhang Ya'nan Guo Tiantian He Taoxiu Lin Xiaona Ma | 2023 | Journal of Traditional Chinese Medical Sciences2023，10，2： | 1 |
| 11 | The design of HEPS global timing system显示文摘Purpose The high energy photon source(HEPS)is a 4th generation synchrotron light source under construction by the institute of high energy physics.The accelerator complex consists of a 500-MeV Linac,a full-energy synchrotron booster,a 6-GeV synchrotron storage ring(SR),and three e-beam transport lines for injection and extraction among accelerators.A global timing system(GTS)covers the timing needs for all accelerator,beamline,and experiment systems.The GTS is designed to coordinate the injection processes and various measurements and protections.Most systems require that the RMS jitter of the GTS signal is less than 30 ps,while the trigger jitters for the electron gun and SR injection and extraction kickers are less than 10 ps.Method The HEPS GTS is an event-based timing system based on MicroTCA.4 hardware architecture.The MicroTCA.4300 series products from the micro-research Finland Oy are implemented in the HEPS GTS system.Results and conclusions The RMS jitter,integrated from 1 Hz to 10 MHz,of the 166.6 MHz event clock is 5.489 ps.The RMS jitter of TTL outputs is less than 30 ps.This paper reports the design of the HEPS GTS,which satisfies all of the HEPS physics requirements for timing with preliminary test results shown. | Fang Liu Ge Lei Zhe Duan Cai Meng Chenyan Lu Yanhua Shao Xinpeng Ma Nan Gan Yuemei Peng Jianshe Cao Chungming Chu Yanfeng Sui Jingyi Li IHEP | 2021 | Radiation Detection Technology and Methods2021，5，3： | 1 |
| 12 | Gene transfer of cocaine hydrolase suppresses cardiovascular responses to cocaine in rats显示文摘 | Gao Yang Atanasova E Sui Nan | 2005 | Mol Pharmacol2005，67，1： | 1 |
| 13 | Effect of SCH23390,spiperone and MK-801 into IMHV and LPO on acquisition of morphineinduced conditioned place preference in day-old chicks显示文摘 | Liu Jie Xiao Lin Sui Nan | | Pharmacol Biochem Behav0，，： | 1 |
| 14 | Identification and Characterization of DPZF, a Novel Human BTB/POZ Zinc Finger Protein Sharing Homology to BCL-6显示文摘 | Weiping Zhang Jing Mi Nan Li Lili Sui Tao Wan Jia Zhang Taoyong Chen Xuetao Cao | 2001 | Biochemical and Biophysical Research Communications2001，，4： | 1 |
| 15 | Apoptotic vesicles ameliorate lupus and arthritis via phosphatidylserine-mediated modulation of T cell receptor signaling显示文摘Mesenchymal stem cells(MSCs)influence T cells in health,disease and therapy through messengers of intercellular communication including extracellular vesicles(EVs).Apoptosis is a mode of cell death that tends to promote immune tolerance,and a large number of apoptotic vesicles(apoVs)are generated from MSCs during apoptosis.In an effort to characterize these apoVs and explore their immunomodulatory potential,here we show that after replenishing them systemically,the apoV deficiency in Fas mutant mice and pathological lymphoproliferation were rescued,leading to the amelioration of inflammation and lupus activity.ApoVs directly interacted with CD4^(+)T cells and inhibited CD25 expression and IL-2 production in a dose-dependent manner.A broad range of Th1/2/17 subsets and cytokines including IFNγ,IL17A and IL-10 were suppressed while Foxp3^(+)cells were maintained.Mechanistically,exposed phosphatidylserine(PtdSer/PS)on apoVs mediated the interaction with T cells to disrupt proximal T cell receptor signaling transduction.Remarkably,administration of apoVs prevented Th17 differentiation and memory formation,and ameliorated inflammation and joint erosion in murine arthritis.Collectively,our findings unveil a previously unrecognized crosstalk between MSC apoVs and CD4^(+)T cells and suggest a promising therapeutic use of apoVs for autoimmune diseases. | Runci Wang Meng Hao Xiaoxing Kou Bingdong Sui Maria Laura Sanmillan Xiao Zhang Dawei Liu Jun Tian Wenjing Yu Chider Chen Ruili Yang Lingyun Sun Yi Liu Claudio Giraudo Deepak A.Rao Nan Shen Songtao Shi | 2023 | Bioactive Materials2023，，7： | 1 |
| 16 | hSSTR2 expression and octreotide treatment reverses multidrug resistance of BxPC-3 human pancreatic cancer cells显示文摘 | Sui C Ma Q Nan K | | 0，，06： | 1 |
| 17 | An animated landscape representation of CD4+ T‐cell differentiation, variability, and plasticity: Insights into the behavior of populations versus cells显示文摘 | Jonathan A. Rebhahn Nan Deng Gaurav Sharma Alexandra M. Livingstone Sui Huang Tim R. Mosmann | 2014 | Immunol2014，，8： | 1 |
| 18 | Microstructures and hardness of Ti-6Al-4V alloy casting under centrifugal field显示文摘 | SUI Yan-wei LI Bang-sheng LIU Ai-hui NAN Hai GUO Jing-jie FU Heng-zhi | 2008 | Trans Nonferrous Met Soc China2008，18，： | 1 |
| 19 | Targeted inhibition of osteoclastogenesis reveals the pathogenesis and therapeutics of bone loss under sympathetic neurostress显示文摘Sympathetic cues via the adrenergic signaling critically regulate bone homeostasis and contribute to neurostress-induced bone loss,but the mechanisms and therapeutics remain incompletely elucidated.Here,we reveal an osteoclastogenesis-centered functionally important osteopenic pathogenesis under sympatho-adrenergic activation with characterized micro RNA response and efficient therapeutics.We discovered that osteoclastic mi R-21 was tightly regulated by sympatho-adrenergic cues downstream theβ2-adrenergic receptor(β2AR)signaling,critically modulated osteoclastogenesis in vivo by inhibiting programmed cell death 4(Pdcd4),and mediated detrimental effects of both isoproterenol(ISO)and chronic variable stress(CVS)on bone.Intriguingly,without affecting osteoblastic bone formation,bone protection against ISO and CVS was sufficiently achieved by a(D-Asp8)-lipid nanoparticle-mediated targeted inhibition of osteoclastic mi R-21 or by clinically relevant drugs to suppress osteoclastogenesis.Collectively,these results unravel a previously underdetermined molecular and functional paradigm that osteoclastogenesis crucially contributes to sympatho-adrenergic regulation of bone and establish multiple targeted therapeutic strategies to counteract osteopenias under stresses. | Bingdong Sui Jin Liu Chenxi Zheng Lei Dang Ji Chen Yuan Cao Kaichao Zhang Lu Liu Minyan Dang Liqiang Zhang Nan Chen Tao He Kun Xuan Fang Jin Ge Zhang Yan Jin Chenghu Hu | 2022 | International Journal of Oral Science2022，14，4： | 1 |
| 20 | Existence and features of the myodural bridge in gentoo penguins:a morphological study显示文摘Recent studies have evidenced that the anatomical structure now known as the myodural bridge(MDB)connects the suboccipital musculature to the cervical spinal dura mater(SDM).In humans,the MDB passes through both the posterior atlanto-occipital and the posterior atlanto-axial interspaces.The existence of the MDB in various mammals,including flying birds(Rock pigeons and Gallus domesticus)has been previously validated.Gentoo penguins are the world's fastest diving birds.The present study was therefore carried out to investigate the existence and characteristics of the MDB in Gentoo penguin,a non-flying,marine bird that can dive.For this study,six Gentoo penguin specimens were dissected to observe the existence and composition of their MDB. | Cheng Chen Yu Shengbo Chi Yanyan Tan Guangyuan Yan Baocheng Zheng Nan Sui Hongjin | 2021 | 解剖学杂志2021，44，S01： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员