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| 1 | Gene expression profiling and endothelin in acute experimental pancreatitis显示文摘AIM:To analyze gene expression profiles in an experimental pancreatitis and provide functional reversal of hypersensitivity with candidate gene endothelin-1 antagonists.METHODS:Dibutyltin dichloride(DBTC) is a chemical used as a polyvinyl carbonate stabilizer/catalyzer,biocide in agriculture,antifouling agent in paint and fabric.DBTC induces an acute pancreatitis flare through generation of reactive oxygen species.Lewis-inbred rats received a single i.v.injection with either DBTC or vehicle.Spinal cord and dorsal root ganglia(DRG) were taken at the peak of inflammation and processed for transcriptional profiling with a cDNA microarray biased for rat brain-specific genes.In a second study,groups of animals with DBTC-induced pancreatitis were treated with endothelin(ET) receptor antagonists [ET-A(BQ123) and ET-B BQ788)].Spontaneous pain related mechanical and thermal hypersensitivity were measured.Immunohistochemical analysis was performed using anti-ET-A and ET-B antibodies on sections from pancreatic tissues and DRG of the T10-12 spinal segments.RESULTS:Animals developed acute pancreatic inflammation persisting 7-10 d as confirmed by pathological studies(edema in parenchyma,loss of pancreatic architecture and islets,infiltration of inflammatory cells,neutrophil and mononuclear cells,degeneration,vacuolization and necrosis of acinar cells) and the painrelated behaviors(cutaneous secondary mechanical and thermal hypersensitivity).Gene expression profile was different in the spinal cord from animals with pancreatitis compared to the vehicle control group.Over 260 up-regulated and 60 down-regulated unique genes could be classified into 8 functional gene families:circulatory/acute phase/immunomodulatory;extracellular matrix;structural;channel/receptor/transporter;signaling transduction;transcription/translation-related;antioxidants/chaperones/heat shock;pancreatic and other enzymes.ET-1 was among the 52 candidate genes upregulated greater than 2-fold in animals with pancreatic inflammation and visceral pain-related behavior.Treatments with the ET-A(BQ123) and ET-B(BQ-788) antagonists revealed significant protection against inflammatory pain related mechanical and thermal hypersensitivity behaviors in animals with pancreatitis(P < 0.05).Open field spontaneous behavioral activity(at baseline,day 6 and 30 min after drug treatments(BQ123,BQ788) showed overall stable activity levels indicating that the drugs produced no undesirable effects on normal exploratory behaviors,except for a trend toward reduction of the active time and increase in resting time at the highest dose(300 μmol/L).Immunocytochemical localization revealed that expression of ET-A and ET-B receptors increased in DRG from animals with pancreatitis.Endothelin receptor localization was combined in dual staining with neuronal marker NeuN,and glia marker,glial fibrillary acidic protein.ET-A was expressed in the cell bodies and occasional nuclei of DRG neurons in na ve animals.However,phenotypic expression of ET-A receptor was greatly increased in neurons of all sizes in animals with pancreatitis.Similarly,ET-B receptor was localized in neurons and in the satellite glia,as well as in the Schwann cell glial myelin sheaths surrounding the axons passing through the DRG.CONCLUSION:Endothelin-receptor antagonists protect against inflammatory pain responses without interfering with normal exploratory behaviors.Candidate genes can serve as future biomarkers for diagnosis and/or targeted gene therapy. | Helieh S Oz Ying Lu Louis P Vera-Portocarrero Pei Ge Ada Silos-Santiago Karin N Westlund | 2012 | World Journal of Gastroenterology2012，18，32： | 6 |
| 2 | Improved clinical outcome using polytetrafluoroethylene-coated stents for tips: Results of a randomized study显示文摘 | Christophe Bureau Juan carlos Garcia-Pagan Philippe Otal Gilles Pomier-Layrargues Valérie Chabbert Carlos Cortez Pierre Perreault Jean marie Péron Juan G. Abraldes Louis Bouchard José Ignacio Bilbao Jaume Bosch Hervé Rousseau Jean pierre Vinel | 2004 | Gastroenterology2004，，2： | 3 |
| 3 | Plasticity of Human Adipose Lineage Cells Toward Endothelial Cells: Physiological and Therapeutic Perspectives显示文摘 | Valérie Planat-Benard Jean-Sébastien Silvestre Béatrice Cousin Mireille André Maryse Nibbelink Radia Tamarat Michel Clergue Carole Manneville Corinne Saillan-Barreau Micheline Duriez Alain Tedgui Bernard Levy Luc Pénicaud Louis Casteilla | 2004 | Circulation: Journal of the American Heart Association2004，，5： | 2 |
| 4 | Evidence That the Diabetes Gene Encodes the Leptin Receptor: Identification of a Mutation in the Leptin Receptor Gene in db / db Mice显示文摘 | Hong Chen Olga Charlat Louis A Tartaglia Elizabeth A Woolf Xun Weng Stephen J Ellis Nathan D Lakey Janice Culpepper Karen J More Roger E Breitbart Geoffrey M Duyk Robert I Tepper Jay P Morgenstern | 1996 | Cell1996，，3： | 2 |
| 5 | Lactate utilizing bacteria,isolated from human faeces,that produce butyrate as a major fermentation product显示文摘 | Duncan S H Louis P Flint H J | 2004 | Appl Environ Microbiol2004，70，： | 1 |
| 6 | 查看详情显示文摘 | Louis B Ocampo F Yun H S Twssonnier J P Pereira M M | | 0，，： | 1 |
| 7 | Understanding the new allele prob-lem显示文摘 | Klitz W Hedrick P Louis E | 2011 | Tissue Antigens2011，77，5： | 1 |
| 8 | Procalcitonin and C--reactive protein as markers of bacterial infection in critically ill children at onset of systemic inflammatory response syndrome 显示文摘 | Simon L Saint-- Louis P Amre DK | 2008 | Pediatr CritCare Med2008，9，4： | 1 |
| 9 | A review of volatile analytical methods for determining the botanical origin of honey 显示文摘 | Luis F C G Jorge A P Louis S S | 2007 | Food Chem2007，103，： | 1 |
| 10 | The WHO classification of tumors of the nervous system显示文摘 | Kleihues P Louis DN Scheithauer BW | 2002 | J Nenropathol Exp Neurol2002，61，3： | 1 |
| 11 | NOD2/CARD15 does not influence response to Infliximab in Crohn' s disease显示文摘 | Vermeire S Louis E Rutgeerts P | 2002 | Gastroenterology2002，123，： | 1 |
| 12 | CRP1,a LIM domainprotein implicated in muscle differentiation,interacts with alphaactinin显示文摘 | Pomies P Louis H A Beckerle M C | 1997 | The Journal of cell biology1997，139，1： | 1 |
| 13 | Using real- world data tbr coverage and payment decisions: the ISPOR real- world data task force report 显示文摘 | LOUIS P G J PETER J N PENNIFER E | 2007 | Value Health2007，10，5： | 1 |
| 14 | The WHO classification of tumors of the nervous system显示文摘 | Kleihues P Louis DN Scheithauer BW | 2002 | J Neuropathol and Exp Neurol2002，61，： | 1 |
| 15 | Computer simulation of spatial arrangement and connectivity of particles in 3D microstructure: Application to model electrical conductivity of a polymer matrix composite 显示文摘 | Louis P Gokhale A M | 1996 | Acta Mater1996，44，4： | 1 |
| 16 | Preclinical and clinical evaluations of ABX-EGF, a fully human anti-epidermal growth factor receptor antibody显示文摘 | Kenneth A Foon Xiao-Dong Yang Louis M Weiner Arie S Belldegrun Robert A Figlin Jeffrey Crawford Eric K Rowinsky Janice P Dutcher Nicholas J Vogelzang Jared Gollub John A Thompson Garry Schwartz Ronald M Bukowski Lorin K Roskos Gisela M Schwab | 2004 | International Journal of Radiation Oncology Biology Physics2004，，3： | 1 |
| 17 | Hamiltonian circuits in random graphs 显示文摘 | Pósa Louis | 1976 | Discrete Mathematics1976，14，4： | 1 |
| 18 | Tricuspid valve replacement: an analysis of 25 years of experience at a single center显示文摘 | Michel Carrier Yves Hébert Michel Pellerin Denis Bouchard Louis P Perrault Raymond Cartier Arsène Basmajian Pierre Pagé Nancy C Poirier | 2003 | The Annals of Thoracic Surgery2003，，1： | 1 |
| 19 | Giant fusiform aneurysm of the cerebral arteries 显示文摘 | LITTLE J R ST LOUIS P WEINSTEIN M | 1981 | Stroke1981，12，2： | 1 |
| 20 | Current strategies for dentist-applied treatment in the management of hypersensitive dentine 显示文摘 | Louis P Gangarosa LP | 1994 | Arch Oral Bioi1994，，： | 1 |
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