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| 1 | A Robust CRISPR/Cas9 System for Convenient, High-Efficiency Multiplex Genome Editing in Monocot and Dicot Plants显示文摘CRISPR/Cas9 染色体指向系统被用于许多种类。然而,为植物报导的 mostCRISPR/Cas9 系统能仅仅修改一个或一些指向地点。这里,我们报导一个柔韧的 CRISPR/Cas9 向量系统,利用植物 codon 优化了 Cas9 基因,为方便、高效率的复合染色体在 monocot 和 dicot 植物编辑。我们设计了基于 PCR 的过程很快产生多重 sgRNA 表达式盒子,它能在一个轮由金门结扎或吉布森汇编克隆被装配进二进制 CRISPR/Cas9 向量。与这个系统,我们与 85.4% 变化评估的一般水准在米饭编辑了 46 个目标地点,主要在 biallelic 和同型结合的地位。我们推想在米饭的大约 16% 同型结合的变化通过相应基于再结合的修理跟随的非相应的加入结束的机制被产生。我们也获得了一致 biallelic,在 Arabidopsis T 1 植物的异质接合、同型结合、妄想的变化。在米饭和 Arabidopsis 的指向的变化是可继承的。我们在 T 0 米饭和 T 1 由同时的指向的 Arabidopsis 植物多重(多达八) 一个基因家庭,在一条 biosynthetic 小径的多重基因,或在单个基因的多重地点的成员。这个系统为为基本研究和基因改进在植物学习多重基因和基因家庭的功能提供了一个万用的工具箱。 | Xingliang Ma Qunyu Zhang Qinlong Zhu Wei Liu Yan Chen Rong Qiu Bin Wang Zhongfang Yang Heying Li Yuru Lin Yongyao Xie Rongxin Shen Shuifu Chen Zhi Wang Yuanling Chen Jingxin Guo Letian Chen Xiucai Zhao Zhicheng Dong Yao-Guang Liu | 2015 | Molecular Plant2015，8，8： | 272 |
| 2 | Design Bistatic Interferometric DEM Generation Algorithm and Its Theoretical Accuracy Analysis for LuTan-1 Satellites显示文摘LuTan-1(LT-1)is a constellation with two full-polarization L-band radar satellites designed by China,and the first satellite was scheduled to be launched in December 2021 and the second one in January 2022.The LT-1 will be operated for deformation monitoring in repeat-pass mode,and for DEM generation in bistatic mode,improving self-sufficiency of SAR data for the field of geology,earthquake,disaster reduction,geomatics,forestry and so on.In this paper,we focused on designing an algorithm for interferometric DEM generation using LT-1 bistatic satellites.The basic principle,main error sources and errors control of the DEM generation algorithm of LT-1 were systematically analyzed.The experiment results demonstrated that:①The implemented algorithm had rigorous resolution with a theoretic accuracy better than 0.03 m for DEM generation.②The errors in satellite velocity and Doppler centroid had no obvious effect on DEM accuracy and they could be neglected.While the errors in position,baseline,slant range and interferometric phase had a significant effect on DEM accuracy.And the DEM error caused by baseline error was dominated,followed by the slant range error,interferometric phase error and satellite position error.③To obtain an expected DEM accuracy of 2 m,the baseline error must be strictly controlled and its accuracy shall be 1.0 mm or better for Cross-Track and Normal-Direction component,respectively.And the slant range error and interferometric phase error shall be reasonably controlled.The research results were of great significance for accurately grasping the accuracy of LT-1 data products and their errors control,and could provide a scientific auxiliary basis for LT-1 in promoting global SAR technology progress and the generation of high-precision basic geographic data. | Bing XU Liqun LIU Zhiwei LI Yan ZHU Jingxin HOU Wenxiang MAO | 2022 | Journal of Geodesy and Geoinformation Science2022，5，1： | 6 |
| 3 | Transarterial chemoembolization in combination with programmed death-1/programmed cell death-ligand 1 immunotherapy for hepatocellular显示文摘Hepatocellular carcinoma(HCC)is one of the most common malignancies worldwide.With the development of systemic therapies,several studies are currently underway,especially those related to the use of programmed death-1/programmed cell death-ligand 1(PD-1/PD-L1)immunotherapy.Moreover,studies on transarterial che-moembolization(TACE)and PD-1/PD-L1 immunotherapy have demonstrated some interesting outcomes.This article reviewed the current clinical evidence on the combination of TACE and PD-1/PD-L1 immunotherapy.Overall,our review summarized that a favorable survival time could be achieved using this combination in most patients.However,complications such as hyperprogression should be taken seriously,and the underlying mechanisms need to be explored. | Jingxin Yan Manjun Deng Shunyu Kong Ting Li Zhenwu Lei Lushun Zhang Yutong Zhuang Xin He Huanwei Wang Haining Fan Yingxing Guo | 2022 | iLIVER2022，1，4： | 2 |
| 4 | Efficacy and complications of transarterial chemoembolization alone or in combination with different protocols for hepatocellular carcinoma: A Bayesian network meta-analysis of randomized controlled trials显示文摘Background and aims:Transarterial chemoembolization(TACE)is the standard treatment for most intermediate-toadvanced stage hepatocellular carcinoma.With the ongoing development of interventional therapies,TACE in combination with different protocols is being explored.A network meta-analysis to collect recent evidence in this field is required.Methods:We searched PubMed,Web of Science,Cochrane Central Register of Controlled Trials,Embase,Chinese National Knowledge Infrastructure database,Cochrane Library,and Wanfang database from their inception to May 2022 for randomized controlled trials(RCTs)that reported TACE in combination with different protocols for hepatocellular carcinoma.Results:In total,67 RCTs assessing eight combination treatments were included.The quality of primary outcomes was from moderate to low.The Bayesian network meta-analysis confirmed that TACE combined with iodine-125 seed implantation had the highest probability of 1-year survival rate and TACE combined with sorafenib had the highest probability of 2-year survival rate.TACE alone had the highest probability of incidence of fever,TACE combined with sorafenib had the highest probability of incidence of pain and TACE combined with percutaneous ethanol injection had the highest probability of incidence of nausea and vomiting.Conclusion:This Bayesian network meta-analysis found that TACE combined with iodine-125 seed and TACE combined with sorafenib may benefit patients in terms of 1-year survival and 2-year survival,respectively.To confirm this conclusion,high-quality and well-designed RCTs with a larger sample size are needed. | Jingxin Yan Manjun Deng Ting Li Changhao Dong Minglan Wang Shunyu Kong Yingxing Guo Haining Fan | 2023 | iLIVER2023，2，2： | 1 |
| 5 | The study of non-uniformity correction algorithm for IRFPA based on neural network显示文摘 | Yan Yi Hong Jingxin Wang Wenyin | 2008 | Computer Science and Software Engineering2008，334，9： | 1 |
| 6 | A broadly neutralizing human monoclonal antibody against the hemagglutinin of avian influenza virus H7N9显示文摘Background:The new emerging avian influenza A H7N9 virus,causing severe human infection with a mortality rate of around 41%.This study aims to provide a novel treatment option for the prevention and control of H7N9.Methods:H7 hemagglutinin(HA)-specific B cells were isolated from peripheral blood plasma cells of the patients previously infected by H7N9 in Jiangsu Province,China.The human monoclonal antibodies(mAbs)were generated by amplification and cloning of these HA-specific B cells.First,all human mAbs were screened for binding activity by enzyme-linked immunosorbent assay.Then,those mAbs,exhibiting potent affinity to recognize H7 HAs were further evaluated by hemagglutination-inhibiting(HAI)and microneutralizationin vitro assays.Finally,the lead mAb candidate was selected and tested against the lethal challenge of the H7N9 virus using murine models.Results:The mAb 6-137 was able to recognize a panel of H7 HAs with high affinity but not HA of other subtypes,including H1N1 and H3N2.The mAb 6-137 can efficiently inhibit the HA activity in the inactivated H7N9 virus and neutralize 100 tissue culture infectious dose 50(TCID_(50))of H7N9 virus(influenza A/Nanjing/1/2013)invitro,with neutralizing activity as low as 78 ng/mL.In addition,the mAb 6-137 protected the mice against the lethal challenge of H7N9 prophylactically and therapeutically.Conclusion:The mAb 6-137 could be an effective antibody as a prophylactic or therapeutic biological treatment for the H7N9 exposure or infection. | Jingxin Li Li Zhang Linlin Bao Yuxiao Wang Lin Qiu Jialei Hu Rong Tang Huiyan Yu Jun Shan Yan Li Chuan Qin Fengcai Zhu | 2022 | Chinese Medical Journal2022，，7： | 1 |
| 7 | Liquefaction of bamboo biomass and production of three fractions containing aromatic compounds显示文摘Depolymerization of lignin to produce value-added aromatic monomers has attracted increasing attention since these monomers can be used as phenol replacement in production of phenolic resins.Here a one-pot depolymerization of bamboo lignin was investigated to obtain aromatic platforms with low molecular weight using acidic catalyst and ethanol.Three fractions(1#,2#,and 3#)containing different molecular weight distributions of aromatic compounds could be efficiently extracted using water-organic solvent system via a stepwise fractionation process by gradual removal of solvent.The fractions distribution was found to be primarily dependent on the reaction temperature and time.When the temperature was increased from 160°C to 200°C,the yield of fractions containing aromatic products increased significantly from 19.1 wt%to 27 wt%,the same change trend was found by changing the time,and the yield of aromatic products increased from 22.4%to 26.7%with an increase of time from 10 min to 30 min.The bioproducts were characterized by using gas chromatography/mass spectrometry(GC-MS),gel permeation chromatography(GPC)and two-dimensional heteronuclear single-quantum coherence(2D HSQC NMR).As evidenced by GC-MS spectra,the three fractions were mainly comprised of phenolic derivatives,and the relative contents of phenolic compounds took up about 80%of the total area of each fraction.With the similar physiochemical properties of the fractions,aromatic platforms could provide a new paradigm of bamboo lignin utilization for renewable energy and value-added biochemicals. | Yan Ma Weihong Tan Jingxin Wang Junming Xu Kui Wang Jianchun Jiang | 2020 | Journal of Bioresources and Bioproducts2020，5，2： | 0 |
| 8 | Effect of Welding Seam Spacing and Relative Bar Wall Thickness of Bus Framework on Joint’s Local Mechanical Property显示文摘Now the researches concerning integral bus mainly focused on design and analysis of overall mechanical property of bus body,and paid little attention to characteristic of local structures,such as joint,plug welding hole,and bolt connection point,etc.So there is much blindness on the design of local connecting structure.Since integral bus body structure cancels large section longitudinal beam,and uses framework made by welding small section bars together as principal part to bear the whole load when the vehicle works,there are many joints receiving high load in the body structure,and local stress concentration can not be avoided.Under such circumstances,by adopting beam-shell mixed model based on super element technique,and selecting a joint commonly used by bus sidewall,the rule of the effect of bar joint’s welding seam spacing on joint's local mechanical property is investigated in this paper,and the investigating results show that joints have minimum stress concentration with welding seam spacing of 8 mm.To learn whether the above rule is affected by relative bar wall thickness,many groups of bars with different relative bar wall thicknesses are studied experimentally,and the experimental results show that the joints local stress levels vary with different relative bar wall thicknesses,but the rule of the effect of bar joint's welding seam spacing on joint's local stress level remains the same.The research is significant for local structure design of bus joint in the future. | NA Jingxin YAN Yakun LIU Haipeng | 2012 | Chinese Journal of Mechanical Engineering2012，25，1： | 0 |
| 9 | A MEMBRANE ELEMENT MODEL WITH BENDING MODIFICATION FOR ONE STEP INVERSE METHOD显示文摘A membrane element model with bending modification based on element moment equilibrium is proposed for the first time by the authors,who apply the element model in one step inverse method and simulate the forming process of a flower-shaped box using the membrane element model with and without this modification.The numerical results are compared with those of the incremental method to verify the validity of the element model developed in this paper. | Jingxin Na JianJiao Yakun Yan Haipeng Liu | 2011 | Acta Mechanica Solida Sinica2011，24，3： | 0 |
| 10 | Engineering DNA quadruplexes in DNA nanostructures for biosensor construction显示文摘DNA quadruplexes are nucleic acid conformations comprised of four strands.They are prevalent in human genomes and increasing efforts are being directed toward their engineering.Taking advantage of the programmability of Watson-Crick base-pairing and conjugation methodology of DNA with other molecules,DNA nanostructures of increasing complexity and diversified geometries have been artificially constructed since 1980s.In this review,we investigate the interweaving of natural DNA quadruplexes and artificial DNA nanostructures in the development of the ever-prosperous field of biosensing,highlighting their specific roles in the construction of biosensor,including recognition probe,signal probe,signal amplifier and support platform.Their implementation in various sensing scenes was surveyed.And finally,general conclusion and future perspective are discussed for further developments. | Jingxin Liu Li Yan Shiliang He Junqing Hu | 2022 | Nano Research2022，15，4： | 0 |
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