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| 1 | A Robust CRISPR/Cas9 System for Convenient, High-Efficiency Multiplex Genome Editing in Monocot and Dicot Plants显示文摘CRISPR/Cas9 染色体指向系统被用于许多种类。然而,为植物报导的 mostCRISPR/Cas9 系统能仅仅修改一个或一些指向地点。这里,我们报导一个柔韧的 CRISPR/Cas9 向量系统,利用植物 codon 优化了 Cas9 基因,为方便、高效率的复合染色体在 monocot 和 dicot 植物编辑。我们设计了基于 PCR 的过程很快产生多重 sgRNA 表达式盒子,它能在一个轮由金门结扎或吉布森汇编克隆被装配进二进制 CRISPR/Cas9 向量。与这个系统,我们与 85.4% 变化评估的一般水准在米饭编辑了 46 个目标地点,主要在 biallelic 和同型结合的地位。我们推想在米饭的大约 16% 同型结合的变化通过相应基于再结合的修理跟随的非相应的加入结束的机制被产生。我们也获得了一致 biallelic,在 Arabidopsis T 1 植物的异质接合、同型结合、妄想的变化。在米饭和 Arabidopsis 的指向的变化是可继承的。我们在 T 0 米饭和 T 1 由同时的指向的 Arabidopsis 植物多重(多达八) 一个基因家庭,在一条 biosynthetic 小径的多重基因,或在单个基因的多重地点的成员。这个系统为为基本研究和基因改进在植物学习多重基因和基因家庭的功能提供了一个万用的工具箱。 | Xingliang Ma Qunyu Zhang Qinlong Zhu Wei Liu Yan Chen Rong Qiu Bin Wang Zhongfang Yang Heying Li Yuru Lin Yongyao Xie Rongxin Shen Shuifu Chen Zhi Wang Yuanling Chen Jingxin Guo Letian Chen Xiucai Zhao Zhicheng Dong Yao-Guang Liu | 2015 | Molecular Plant2015，8，8： | 272 |
| 2 | Temporal variation of soil carbon stock and its controlling factors over the last two decades on the southern Song-nen Plain,Heilongjiang Province显示文摘对全球气候变化的当前的背景,在土壤碳水池和它的控制因素的变化的学习可以在温室气体的缓解或改进帮助土壤角色的评估。这份报纸在 Heilongjiang 省在南部的 Song-nen 平原在土壤碳水池和他们的控制因素学习空间、时间的变化,用在二个不同时期由上收集的土壤数据在 20052007 的多种用途的地区性的 Geochemical 调查,和另外一个玷污在 19821990 进行的调查。学习区域是 1479 t/km2 并且在过去的 20 年里的碳来源,从直到 2005 的 1980 年代,从土壤的实际的碳排放是 0.12 Gt。温度,被发现了线性地被相关玷污器官的碳,是控制器官的碳满足的土壤的主导的 climatologic 因素。我们的学习在器官的碳由升起的温度引起了的土壤的潜在的损失是的相关区域和时间时期显示出那 0.10 Gt ,源于陆地使用变化的潜在的土壤碳排放是 0.09 Gt ,并且温暖和陆地使用变化引起的土壤碳( 0.19 Gt )的联合潜在的损失比得上石块燃料燃烧( 0.21 Gt )的。由于在土壤碳水池变化的时间延期,仍然在温暖和以后将逐渐地被释放的陆地使用变化引起的潜在的排放有 0.07 Gt。 | Xueqi Xia Zhongfang Yang Yan Liao Yujun Cui Yansheng Li | 2010 | Geoscience Frontiers2010，1，1： | 19 |
| 3 | Seasonal variations in the Sr-Nd isotopic compositions of suspended particulate matter in the lower Changjiang River:Provenance and erosion constraints显示文摘Suspended particulate matter samples were collected monthly for more than 2 years in Nanjing,China to examine seasonal changes in the Sr-Nd isotopic compositions of the lower Changjiang River(CR).The results indicate that the 87Sr/86Sr ratios of the samples ranges from 0.725352 to 0.738128,and the values ofεNd(0)ranges from-10.55 to-12.29.The Sr-Nd isotopic compositions show distinct seasonal variations.The samples had lower 87Sr/86Sr ratios and higherε Nd(0)values during the flood season than the dry seasons.The seasonal variations primarily reflect the controls of provenance rocks and erosion in different sub-catchments.The relative decrease in 87Sr/86Sr ratios and the increase inε Nd(0)values during the flood season may reflect an increasing in the mechanical erosion rate in the upper basin and the contribution of more sediment from the upper reaches.The end member values of 87Sr/86Sr andε Nd(0)of the samples were 0.728254 and?11.26,respectively. | MAO ChangPing CHEN Jun YUAN XuYin YANG ZhongFang JI JunFeng | 2011 | Chinese Science Bulletin2011，56，22： | 7 |
| 4 | An effective strategy to establish a male sterility mutant mini-library by CRISPR/Cas9-mediated knockout of anther-specific genes in rice显示文摘Male sterility(MS),characterized by functional defects in male organs or gametes,is an important agronomic trait for hybrid seed production,especially for self-pollinated crops such as rice(Oryza sativa L)(Chen and Liu,2014).Spontaneous MS mutants are rare and difficult to maintain in nature,thus limiting basicresearch and breeding.Artificial mutants are typically generated by physical,chemical,or biological mutagenesis(Wei et al,2013).Recently developed genome editing systems such as CRISPR/Cas9 allow efficient and timesaving knockout of endogenous genes at specific sites(Smith et al,2000;Moscou and Bogdanove,2009;Gasiunas et al.,2012). | Kun Ma Jingluan Han Yu Hao Zhongfang Yang Junyu Chen Yao-Guang Liu Qinlong Zhu Letian Chen | 2019 | Journal of Genetics and Genomics2019，46，5： | 6 |
| 5 | Fucoxanthin induces growth arrest and apoptosis in human bladder cancer T24 cells by up-regulation of p21 and down-regulation of mortalin显示文摘Fucoxanthin,自然类胡萝卜素,被报导了在人的结肠癌房间,人的前列腺癌症房间,人的白血病房间,和人的上皮的颈的癌症房间举办反癌症活动。这研究被承担把 fucoxanthin 的分子的机制与人的膀胱癌症 T24 房间线作比较。 MTT 分析结果证明 5 和 10 M fucoxanthin 在生长拘捕在 G 0/G1伴随的一个剂量依赖者和时间依赖者举止禁止了 T24 房间的增长房间周期的阶段,被 p21 的起来规定调停,cyclin依赖的 kinase ( CDK )禁止的蛋白质和 CDK-2 , CDK-4 , cyclin D1 ,和 cyclin E 的下面规定。另外, 20 和 40 M fucoxanthin 由 mortalinp53 建筑群的废除和原子变异类型的 p53 的复活导致了 T24 房间的 apoptosis,它也让肿瘤 suppressor 作为野类型的 p53 工作。所有这些结果证明 T24 房间上的 fucoxanthin 的反癌症活动被 p21 的起来规定经由在 mortalin ,它是的表示水平,一个压力管理者和热的一个成员震惊的减少在低剂量和 apoptosis 在 G 0/G1阶段与房间周期拘捕联系蛋白质 70 ,在高剂量由劈开的 caspase-3 的起来规定列在后面。 | Linbo Wang Yang Zeng Ye Liu Xuansheng Hu Shuhong Li Yuepeng Wang Ling Li Zhongfang Lei Zhenya Zhang | 2014 | Acta Biochimica et Biophysica Sinica2014，46，10： | 5 |
| 6 | Ecological risk assessment of Cd and other heavy metals in soil-rice system in the karst areas with high geochemical background of Guangxi,China显示文摘Karst areas are widely distributed in China,and the soil derived in these areas is enriched with trace elements and has the typical characteristics of high geochemical background.Therefore,soil ecological risk has gradually attracted extensive attention.In this study,1090 sets of rice grains and paired rhizosphere soils were collected as research objects from the outcropped karst areas of carbonate rocks in Guangxi,and the concentration characteristics and influencing factors of heavy metals,such as Cd and As,in rice grains and the corresponding soils were studied.According to the Chinese Soil Environmental Quality Standard(GB 15618-2018),the proportion of karstic soil samples in Guangxi with heavy metal(As,Cd,Cr,Cu,Hg,Ni,Pb and Zn)concentrations that exceed the standard risk screening values was generally greater than that of surface soils in China or non-karstic soils in Guangxi.Notably,Cd and As were the most prominently enriched heavy metals in soils.According to the Chinese Food Safety Standard(GB 2762-2017),Pb in rice grains from the studied area did not exceed the standard;the exceedance rates of Cr,Hg,and inorganic As were only about 1%;and the exceedance rate of Cd was 12.5%.Soil Cd concentration increased gradually whereas the concentration and exceedance rate of Cd in rice grains decreased gradually with the increase of soil pH.Specifically,the exceedance rate of Cd in rice grains was only 4%when soil pH>6.5.The results indicated that the risk screening values of Cd element in the Chinese Soil Environmental Quality Standard are not satisfactorily applicable to the ecological risk assessment of Cd in karstic soils.Therefore,the recommended threshold values for the safe utilization and risk control of Cd in karstic soils was proposed so as to provide a basis for soil environmental quality hierarchization,pollution risk control and land safety zoning of agricultural land in karst areas in China. | Qiong YANG Zhongfang YANG Qizuan ZHANG Xu LIU Xiaoxiong ZHUO Tiansheng WU Lei WANG Xueji WEI Junfeng JI | 2021 | Science China Earth Sciences2021，64，7： | 5 |
| 7 | A study of soil organic carbon distribution and storage in the Northeast Plain of China显示文摘采用联合起来的土壤碳数量(USCA ) 途径,土壤碳存储基于在 20042006 进行的多种用途的地区性的 Geochemical 调查(MRGS ) 越过中国的东北平原被计算。结果显示在表层土(00.2 m ) 的器官的碳(SOC ) 存储,心土(01 m ) 和深土壤(01.8 m ) 是的土壤有 3327.8 t/km2, 12,904.7 t/km2 和 16,157.5 t/km2 的密度的 768.1 山, 2978.4 山和 3729.2 山分别地。而土壤碳密度从东北平原(辽宁)的南部的区域显示出一个清楚的增加趋势,这些价值以表层土特别地与国家一般水准一致,到中间(吉林)和北平原( Heilongjiang )碳密度,从 2284.2 增加了,到 3436.7 和 3861.5 t/km2 ,分别地。与在 19841986 从第二国家土壤调查获得的碳数据(SNSS ) 相比,从 MRGS 的表层土 SOC 存储值被发现由 320.59 山(29.4%) 减少了,与在 20 年的 16.0 山(l.73%) 的平均年度衰落。在平原的南部、中间、北的区域,分别地,土壤碳密度为整个平原与 1389.0 t/km2 的平均价值由 1060.6 t/km2, 1646.4 t/km2 和 1300.2 t/km2 减少了。这些调查结果显示在土壤碳密度的减少根据不同生态系统和陆地使用类型变化了。因此,土壤碳密度的比率被计算以便学习碳,并且进一步探索分发特征在生态系统之间的动态平衡,以及 SOC 的隐遁潜力。 | Xiaohuan Xi Zhongfang Yang Yujun Cui Shumei Sun Chengguang Yu Min Li | 2011 | Geoscience Frontiers2011，2，2： | 5 |
| 8 | Adsorption of cesium from aqueous solution using agricultural residue – Walnut shell: Equilibrium, kinetic and thermodynamic modeling studies显示文摘 | Dahu Ding Yingxin Zhao Shengjiong Yang Wansheng Shi Zhenya Zhang Zhongfang Lei Yingnan Yang | 2013 | Water Research2013，，： | 4 |
| 9 | Spatial analysis of land use change effect on soil organic carbon stocks in the eastern regions of China between 1980 and 2000显示文摘Spatial distributions of 0-20 cm soil carbon sources/sinks caused by land use changes from the year 1980 to 2000 in an area of 2.97 × 10~6 km^2 in eastern China were investigated using a land use dataset from a recent soil geochemical survey.A map of soil carbon sources/sinks has been prepared based on a spatial analysis scheme with GIS.Spatial statistics showed that land use changes had caused 30.7 ± 13.64 Tg of surface soil organic carbon loss,which accounts for 0.33%of the total carbon storage of 9.22 Pg.The net effect of the carbon source was estimated to be ~ 71.49 Tg soil carbon decrease and ~40.80 Tg increase.Land use changes in Northeast China(NE) have the largest impact on soil organic carbon storage compared with other regions.Paddy fields,which were mainly transformed into dry farmland in NE,and constructed land in other regions,were the largest carbon sources among the land use types.Swamp land in NE was also another large soil carbon source when it was transformed into dry farmland or paddy fields.Dry farmland in the NE region formed the largest soil organic carbon sink,as some were transformed into paddy fields,forested land,and other land use types with high SOCD. | Xueqi Xia Zhongfang Yang Yuan Xue Xin Shao Tao Yu Qingye Hou | 2017 | Geoscience Frontiers2017，8，3： | 3 |
| 10 | N-heterocyclic carbene as a promising metal-free electrocatalyst with high efficiency for nitrogen reduction to ammonia显示文摘Electrocatalytic nitrogen reduction reaction(NRR)at ambient conditions holds great promise for sustainably synthesizing ammonia(NH3),while developing highly-efficient,long-term stable,and inexpensive catalysts to activate the inert N≡N bond is a key scientific issue.In this work,on the basis of the concept'N-heterocyclic carbenes(NHCs)',we propose a carbon decorated graphitic-carbon nitride(C/g-C3N4)as novel metal-free NRR electrocatalyst by means of density functional theory(DFT)computations.Our results reveal that the introduced C atom in g-C3N4 surface can be regarded as NHCs and catalytic sites for activating N≡N bond,and are stabilized by the g-C3N4 substrate due to sterically disfavored dimerization.Especially,this NHCs-based heterogeneous catalysis can efficiently reduce the activated N2 molecule to NH3 with a low overpotential of 0.05 V via an enzymatic mechanism.Our work is the first report of NHCs-based electrocatalyst for N2 fixation,thus opening an alternative avenue for advancing sustainable NH3 production. | Hongyan Li Le Yang Zhongxu Wang Peng Jin Jingxiang Zhao Zhongfang Chen | 2020 | Journal of Energy Chemistry2020，29，7： | 3 |
| 11 | Solid-solution partitioning of arsenic(As) in the paddy soil profiles in Chengdu Plain,Southwest China显示文摘预言砷的长期的行为(作为) 在土壤侧面,稳固答案的划分是在在 Chengdu 从农业区域获得的四稻土壤侧面被学习平原,西南中国。稻土壤侧面样品被收集,土壤答案样品被提取。尽管在土壤答案和土壤固体的内容被分析,总计,与土壤固体阶段性质一起。这在土壤,答案在上面的层(020 厘米) 是显著地更高的并且有一个明确的趋势不管采样地点向 40 厘米减少。当在土壤答案的砷的集中减少了时,它在稳固的阶段的内容增加了。基于地的分区系数(Kd ) 为是被计算数量的比率决定作为在土壤固体阶段到是在土壤答案的集中。Kd 价值在垂直样品广泛地变化了并且是与土壤 pH,全部的器官的碳(TOC ) 和总数相关很好。这研究的结果将为在土壤侧面并且在改进农业土壤的管理评估砷的累积趋势是有用的。 | Xiaoyan Yang Qingye Hou Zhongfang Yang Xin Zhang Yijun Hou | 2012 | Geoscience Frontiers2012，3，6： | 2 |
| 12 | Isolation and Preliminary Identification of Broad-spectrum Bacteriocin-producing Lactobacillus Strain CW3显示文摘[Objective]This study aimed to screen and preliminarily identify Lactobacillus strain CW3. [Method]Oxford cup double-plate method was used for preliminary screening of broad-spectrum bacteriocin-producing Lactobacillus strain; after eliminating the interference of organic acid and hydrogen peroxide,antibacterial substances containing protein properties produced by the screened strain were detected; finally,the obtained strain was identified. [Result]After elimination of interference factors such as organic acid and hydrogen peroxide,the fermentation supernatant of strain CW3 could still inhibit the growth of indicator strains; the antibacterial activity of fermentation broth was significantly reduced after treatment with trypsase and pepsase,which indicated that the produced antibacterial substances were bacteriocins. Results of physiological and biochemical identification showed that strain CW3 belonged to Lactobacillus plantarum. [Conclusion]CW3is a broad-spectrum bacteriocin-producing Lactobacillus plantarum strain. | Haoxin LV Weidong WANG Zhongfang TAN Feifei YANG Yingchun JIAO Yanping WANG Zongwei LI | 2014 | Agricultural Biotechnology2014，3，1： | 2 |
| 13 | Ef- ficiency of Transition Metal Modified Akadama Clay on Cesium Removal from Aqueous Solutions 显示文摘 | Ding Dahu Lei Zhongfang Yang Yingnan | 2014 | Chem Eng J2014，236，： | 1 |
| 14 | Effects of Soil Properties on the Transfer of Cadmium from Soil to Wheat in the Yangtze River Delta Region, China—a Typical Industry–Agriculture Transition Area显示文摘 | Cheng Wang Junfeng Ji Zhongfang Yang Lingxiao Chen Patrick Browne Ruilian Yu | 2012 | Biological Trace Element Research2012，，2： | 1 |
| 15 | Bird nest-like zinc oxide nanostructures for sensitive electrochemical glucose biosensor显示文摘In this research,a novel bird nest-like zinc oxide(BN-ZnO)nanostructures were prepared by a simple solvothermal method.A sensitive electrochemical glucose biosensor was for the first time developed based on the immobilization of glucose oxidase(GOx)on nanostructured BN-ZnO modified electrode.The BN-ZnO nanostructure and the resultant biosensor were characterized by scanning electron microscope,X-ray diffraction spectroscopy,Fourier transform infrared spectroscopy,and electrochemical impedance spectroscopy.BN-ZnO nanostructures have large specific surface area and can load large amounts of GOx molecules.Meanwhile,BN-ZnO provides an excellent microenvironment to retain the native bioactivity of enzymes and to promote direct electron transfer between GOx and electrode surface.The proposed biosensor shows a wide linear range of 0.005–1.6 mmol/L,high sensitivity of15.6 mA L mol^(-1)cm^(-2)with a low detection limit of 0.004 mmol/L.The resulting biosensor also shows excellent selectivity,acceptable stability and reproducibility,and can be successfully applied in the detection of glucose in human serum samples at-0.37 V. | Feng Shi Jinming Xu Zhongfang Hu Chuanli Ren Yadong Xue Yongcai Zhang Juan Li Chengyin Wang Zhanjun Yang | 2021 | Chinese Chemical Letters2021，32，10： | 1 |
| 16 | The origin and geochemical cycle of soil selenium in a Se-rich area of China显示文摘 | Tao Yu Zhongfang Yang Yaoyao Lv Qingye Hou Xueqi Xia Haiyan Feng Meng Zhang Lixin Jin Zezhong Kan | 2013 | Journal of Geochemical Exploration2013，，： | 1 |
| 17 | National multi-purpose regional geochemical survey in China显示文摘 | Min Li Xiaohuan Xi Guiyi Xiao Hangxin Cheng Zhongfang Yang Guohua Zhou Jiayu Ye Zhonghui Li | 2013 | Journal of Geochemical Exploration2013，，： | 1 |
| 18 | Annual net input fluxes of heavy metals of the agro-ecosystem in the Yangtze River delta, China显示文摘 | Qingye Hou Zhongfang Yang Junfeng Ji Tao Yu Guoguang Chen Juan Li Xueqi Xia Ming Zhang Xuyin Yuan | 2013 | Journal of Geochemical Exploration2013，，： | 1 |
| 19 | Resonance Rayleigh-scattering method for the determination of proteins with gold nanoparticle probe显示文摘 | LIU Shaopu YANG Zhuo LIU Zhongfang | 2006 | Analytical Biochemistry2006，353，1： | 1 |
| 20 | Long-range gene regulation network of the MGMT enhancer modulates glioma cell sensitivity to temozolomide显示文摘Approximately 80%of primary tumors in the central nervous system are malignant glioma,and>50%of those are diagnosed as glioblastoma(GBM),the most aggressive glioma.Despite treatment advances,GBM prognosis is unsatisfying,and the median survival time of patients is only 14-17 months. | Bohan Chen Anshun He Jinfang Bi Shupeng Sun Yiping Ma Wenbin Wang Dianhao Guo Jun Chen Yuyang Qian Tengfei Shi Guohui Nie Zhongfang Zhao Jiandang Shi Hongzhen Yang Lei Zhang Wange Lu | 2021 | Journal of Genetics and Genomics2021，48，10： | 1 |
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