|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | 42,573 cases of hepatectomy in China: a multicenter retrospective investigation显示文摘Hepatectomy is currently routinely performed in most hospitals in China. China owns the largest population of liver diseases and the biggest number of liver resection cases. A nationwide multicenter retrospective investigation involving 112 hospitals was performed, and focused on liver resection for patients with hepatocellular carcinoma(HCC). 42,573 cases of hepatectomy were enrolled, and 18,275 valid cases of liver resection for HCC patients were selected for statistical analysis. The epidemiology of HCC, distribution of hepatectomy, postoperative complications and prognosis were finally analyzed. In the 18,275 HCC patients,81% had hepatitis B virus infection and 10% had hepatitis C virus infection. 38% of the HCC patients had normal Alphafetoprotein(AFP) level, and other 35% had an AFP level lower than 400 ng mL^(-1). In the study period, 97% of the hepatectomy for HCC were treated with open surgery, and 23.81% had vascular exclusion techniques. The operation time was(191.7±105.6) min,the blood loss was(546.0±562.8) m L, and blood transfusion was(543.0±1,035.2) m L. The median survival for HCC patients was 631 days, with 1-, 3-, and 5-year overall survival of 73.2%, 28.8% and 19.6%, respectively. Liver cirrhosis, multiple nodules,tumor thrombosis and high AFP level were risk factors that affect postoperative survival. | Binhao Zhang Bixiang Zhang Zhiwei Zhang Zhiyong Huang Yifa Chen Minshan Chen Ping Bie Baogang Peng Liqun Wu Zhiming Wang Bo Li Jia Fan Lunxiu Qin Ping Chen Jingfeng Liu Zhe Tang Jun Niu Xinmin Yin Deyu Li Songqing He Bin Jiang Yilei Mao Weiping Zhou Xiaoping Chen | 2018 | Science China(Life Sciences)2018，61，6： | 39 |
| 2 | Kinetics of the hydrogen absorption and desorption processes of hydrogen storage alloys: A review显示文摘High hydrogen absorption and desorption rates are two significant index parameters for the applications of hydrogen storage tanks.The analysis of the hydrogen absorption and desorption behavior using the isothermal kinetic models is an efficient way to investigate the kinetic mechanism.Multitudinous kinetic models have been developed to describe the kinetic process.However,these kinetic models were de-duced based on some assumptions and only appropriate for specific kinetic measurement methods and rate-controlling steps(RCSs),which sometimes lead to confusion during application.The kinetic analysis procedures using these kinetic models,as well as the key kinetic parameters,are unclear for many researchers who are unfamiliar with this field.These problems will prevent the kinetic models and their analysis methods from revealing the kinetic mechanism of hydrogen storage alloys.Thus,this review mainly focuses on the summarization of kinetic models based on different kinetic measurement methods and RCSs for the chemisorption,surface penetration,diffusion of hydrogen,nucleation and growth,and chemical reaction processes.The analysis procedures of kinetic experimental data are expounded,as well as the effects of temperature,hydrogen pressure,and particle radius.The applications of the kinetic models for different hydrogen storage alloys are also introduced. | Qian Li Xi Lin Qun Luo Yuʼan Chen Jingfeng Wang Bin Jiang Fusheng Pan | 2022 | International Journal of Minerals,Metallurgy and Materials2022，29，1： | 9 |
| 3 | CAS9 is a genome mutator by directly disrupting DNA-PK dependent DNA repair pathway显示文摘With its high efficiency for site-specific genome editing and easy manipulation,the clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR associated protein 9(CAS9)system has become the most widely used gene editing technology in biomedical research.In addition,significant progress has been made for the clinical development of CRISPR/CAS9 based gene therapies of human diseases,several of which are entering clinical trials.Here we report that CAS9 protein can function as a genome mutator independent of any exogenous guide RNA(gRNA)in human cells,promoting genomic DNA double-stranded break(DSB)damage and genomic instability.CAS9 interacts with the KU86 subunit of the DNA-dependent protein kinase(DNA-PK)complex and disrupts the interaction between KU86 and its kinase subunit,leading to defective DNA-PK-dependent repair of DNA DSB damage via non-homologous end-joining(NHEJ)pathway.XCAS9 is a CAS9 variant with potentially higher fidelity and broader compatibility,and dCAS9 is a CAS9 variant without nuclease activity.We show that XCAS9 and dCAS9 also interact with KU86 and disrupt DNA DSB repair.Considering the critical roles of DNA-PK in maintaining genomic stability and the pleiotropic impact of DNA DSB damage responses on cellular proliferation and survival,our findings caution the interpretation of data involving CRISPR/CAS9-based gene editing and raise serious safety concerns of CRISPR/CAS9 system in clinical application. | Shuxiang Xu Jinchul Kim Qingshuang Tang Qu Chen Jingfeng Liu Yang Xu Xuemei Fu | 2020 | Protein & Cell2020，11，5： | 8 |
| 4 | Processes of coastal ecosystem carbon sequestration and approaches for increasing carbon sink显示文摘The oceans are the largest carbon pools on Earth, and play the role of a 'buffer' in climate change. Blue carbon, the carbon(mainly organic carbon) captured by marine ecosystems, is one of the important mechanisms of marine carbon storage.Blue carbon was initially recognized only in the form of visible coastal plant carbon sequestration. In fact, microorganisms(phytoplankton, bacteria, archaea, viruses, and protozoa), which did not receive much attention in the past, account for more than 90% of the total marine biomass and are the main contributors to blue carbon. Chinese coastal seas, equivalent to 1/3 of China's total land area, have a huge carbon sink potential needing urgently research and development. In this paper, we focus on the processes and mechanisms of coastal ocean's carbon sequestration and the approaches for increasing that sequestration. We discuss the structures of coastal ecosystems, the processes of carbon cycle, and the mechanisms of carbon sequestration. Using the evolution of coastal ocean's carbon sinks in sedimentary records over geologic times, we also discuss the possible effects of natural processes and anthropogenic activities on marine carbon sinks. Finally, we discuss the prospect of using carbon sequestration engineering for increasing coastal ocean's carbon storage capacity. | ZHANG Yao ZHAO MeiXun CUI Qiu FAN Wei QI JiaGuo CHEN Ying ZHANG YongYu GAO KunShan FAN JingFeng WANG GuangYi YAN ChongLing LU HaoLiang LUO YaWei ZHANG ZiLian ZHENG Qiang XIAO Wei JIAO NianZhi | 2017 | Science China Earth Sciences2017，60，5： | 6 |
| 5 | Genetic Analysis of Gynogenetic and Common Populations of Verasper moseri Using SSR Markers显示文摘The genetic structure and variation of the artificial meio- gy- nogenetic population and common population of barfin flounder (Verasper moseri) were analyzed using eight microsatellite markers. A total of 29 alleles were detected, of which 23 alleles were in the artificial gynogenetic population while 29 alleles were in the control group. The average ob- served heterozygosity (HO) of eight loci in the control group (0.526 8) was several times higher than that (0.185 8) in the gynogenetic population. The results indicate that the genetic diversity of the control group was much higher than that of the gynogenetic population of barfin flounder (Verasper moseri). Most loci significantly deviated from Hardy-Weinberg equilib- rium (HWE) after Bonferroni correction (p <0.005 56) in the gynoge- netic population, while four loci deviated from HWE in the control group. The coefficient of gene differentiation (GST) was 0.131 0, and the genetic distance was 0.171 8 between the two populations, suggesting a signifi- cant genetic differentiation between the two populations. | MA Hongyu YANG Jingfeng SU Pengzhi CHEN Songlin | 2009 | Wuhan University Journal of Natural Sciences2009，14，3： | 5 |
| 6 | MHC polymorphism and disease-resistance to Edwardsiella tarda in six turbot(Scophthalmus maximus) families显示文摘This study examined genetic variation in the major histocompatibility complex(MHC) Class II B gene in turbot(Scophthalmus maximus) by virulent bacterial pathogen challenge.One hundred fry from each of six families were infected with Edwardsiella tarda by intraperitoneal injection.Family mortality ranged from 28.0% to 83.3%.Complete exon 2 and intron 1 sequences of MHC Class II B genes were amplified from five survivor and five non-survivor individuals per family using the clone-sequence method.Thirty-seven sequences from 60 individuals revealed 37 different alleles,25 of which were unique to this study.The 25 unique alleles belonged to 16 major allele types.Nine alleles were used to examine the association between alleles and resistance/susceptibility to disease.Five alleles were present in an individual,suggesting a minimum of three loci or copies of the turbot MHC Class II B gene.The rate of non-synonymous substitution(d N) was 2.30 and 1.58 times higher than synonymous substitution(d S) in the peptide-binding regions(PBR) and non-PBR in whole families,respectively,which suggested balancing selection on exon 2 of the MHC Class II B gene in turbot.One allele,Scma-DBB1*02,was significantly more prevalent in survivor stock than in non-survivor stock(P=0.001).Therefore,this allele might be associated with resistance to bacteria.A second allele,Scma-DBB1*10,was significantly more prevalent in non-survivor stock(P=0.021),and is likely associated with susceptibility to bacteria. | DU Min CHEN SongLin LIU YanHong NIU BaoZhen YANG JingFeng ZHANG Bo | 2012 | Chinese Science Bulletin2012，57，25： | 5 |
| 7 | Nutrient maximums related to low oxygen concentrations in the southern Canada Basin显示文摘The phenomenon of nutrient maximums at 70~200 m occurred only in the region of the Canada Basin among the world oceans. The prevailing hypothesis was that the direct injection of the low-temperature high-nutrient brines from the Chukchi Sea shelf (<50 m) in winter provided the nutrient maximums. However, we found that there are five problems in the direct injection process. Formerly Jin et al. considered that the formation of nutrient maximums can be a process of locally long-term regeneration. Here we propose a regeneration-mixture process. Data of temperature, salinity, oxygen and nutrients were collected at three stations in the southern Canada Basin during the summer 1999 cruise. We identified the cores of the surface, near-surface, potential temperature maximum waters and Arctic Bottom Water by the diagrams and vertical profiles of salinity, potential temperature, oxygen and nutrients. The historical 129I data indicated that the surface and near-surface waters were Pacific-origin, but the waters below the potential temperature maximum core depth was Atlantic-origin. Along with the correlation of nutrient maximums and very low oxygen contents in the near-surface water, we hypothesize that, the putative organic matter was decomposed to inorganic nutrients; and the Pacific water was mixed with the Atlantic water in the transition zone. The idea of the regeneration-mixture process agrees with the historical observations of no apparent seasonal changes, the smooth nutrient profiles, the lowest saturation of CaCO3 above 400 m, low rate of CFC-11 ventilation and 3H-3He ages of 8~18 a around the nutrient maximum depths. | JIN Ming-ming SHI Jiuxin LU Yong CHEN Jianfang GAO Guoping WU Jingfeng ZHANG Haisheng | 2005 | Acta Oceanologica Sinica2005，24，6： | 5 |
| 8 | An energy conduction model for cell image segmentation显示文摘Cell image segmentation is an essential step in cytopathological analysis.Although their execution speed is fast,the results of cell image segmentation by conventional pixel-based,edge-based and continuity-based methods are often coarse.Fine structures in a cell image can be obtained with a method that quickly adjusts the threshold levels.However,the processing time of such a method is usually long and the final results may be sensitive to intensity differences and other factors.In this article,a new energy model is proposed that synthesizes a differential equation from the conventional and level set methods,and utilizes the nonuniformity property of cell images (e.g.cytoplasms are more uneven than the background).The feasibility and robustness of the proposed model was demonstrated by processing relatively complicated background images of both simulated and real cell images. | MA JingFeng BU JiaJun HOU Kai BAO ShangLian CHEN Chun | 2011 | Chinese Science Bulletin2011，56，10： | 4 |
| 9 | Strain hardening of as-extruded Mg-xZn(x=1, 2, 3 and 4 wt%) alloys显示文摘The influence of Zn on the strain hardening of as-extruded Mg-x Zn(x = 1, 2, 3 and 4 wt%) magnesium alloys was investigated using uniaxial tensile tests at 10^(-3)s^(-1) at room temperature. The strain hardening rate,the strain hardening exponent and the hardening capacity were obtained from true plastic stress-strain curves. There were almost no second phases in the as-extruded Mg-Zn magnesium alloys. Average grain sizes of the four as-extruded alloys were about 17.8 μm. With increasing Zn content from 1 to 4 wt%, the strain hardening rate increased from 2850 MPa to 6810 MPa at(б-б_(0.2)) = 60 MPa, the strain hardening exponent n increased from 0.160 to 0.203, and the hardening capacity, Hc increased from 1.17 to 2.34.The difference in strain hardening response of these Mg-Zn alloys might be mainly caused by weaker basal texture and more solute atoms in the α-Mg matrix with higher Zn content. | Chaoyue Zhao Xianhua Chen Fusheng Pan Jingfeng Wang Shangyu Gao Teng Tu Chunquan Liu Jiahao Yao Andrej Atrens | 2019 | Journal of Materials Science & Technology2019，35，1： | 4 |
| 10 | Epicardial adipose tissue: the accomplice implicated in the genesis and maintenance of atrial fibrillation显示文摘 | Fang Mingcheng Chen Yangxin Wang Jingfeng | 2014 | Chinese Medical Journal2014，，14： | 4 |
| 11 | Effect of Ni on the microstructure, mechanical properties and corrosion behavior of MgGd_1Ni_(x) alloys for fracturing ball applications显示文摘The effects of Ni addition on the mechanical properties, corrosion behaviors, and corrosion mechanism of Mg Gd_1 Ni_(x) alloys have been investigated by compressive tests, weight loss, hydrogen evolution, and scanning electron microscopy. The results show that the constitution of the second phase is large dependence on Ni/Gd molar ratios, which can transform from LPSO(long-period stacking ordered)+Mg_5 Gd,LPSO to eutectic phase. In addition, with increasing Ni/Gd molar ratios, the content of second phase increases gradually, while the LPSO phase shows a parabola relationship. Furthermore, the formation of Nicontaining LPSO phase not only can improve the strength but also accelerate the degradation of Mg alloys owing to the galvanic corrosion. The optimal properties with ultimate compressive strength, degradation rate are 340 MPa, 2066 mm/y for Mg Gd1 Ni0.75, respectively, which can meet the engineering application standard of fracturing ball and can be used as candidate materials for fracturing ball. | Kai Ma Shijie Liu Chaoneng Dai Xiuying Liu Jie Ren Yuanlang Pan Yinhong Peng Chen Su Jingfeng Wang Fusheng Pan | 2021 | Journal of Materials Science & Technology2021，，32： | 4 |
| 12 | Sericin can reduce hippocampal neuronal apoptosis by activating the Akt signal transduction pathway in a rat model of diabetes mellitus显示文摘In the present study, a rat model of type 2 diabetes mellitus was established by continuous peritoneal injection of streptozotocin. Following intragastric perfusion of sericin for 35 days, blood glucose levels significantly reduced, neuronal apoptosis in the hippocampal CA1 region decreased, hippocampal phosphorylated Akt and nuclear factor kappa B expression were enhanced, but Bcl-xL/Bcl-2 associated death promoter expression decreased. Results demonstrated that sericin can reduce hippocampal neuronal apoptosis in a rat model of diabetes mellitus by regulating abnormal changes in the Akt signal transduction pathway. | Zhihong Chen Yaqiang He Chengjun Song Zhijun Dong Zhejun Su Jingfeng Xue | 2012 | Neural Regeneration Research2012，7，3： | 4 |
| 13 | Chinese expert consensus on conversion therapy for hepatocellular carcinoma(2021 edition)显示文摘Recent advances in systemic and locoregional treatments for patients with unresectable or advanced hepatocellular carcinoma(HCC)have resulted in improved response rates.This has provided an opportunity for selected patients with initially unresectable HCC to achieve adequate tumor downstaging to undergo surgical resection,a‘conversion therapy’strategy.However,conversion therapy is a new approach to the treatment of HCC and its practice and treatment protocols are still being developed.Review the evidence for conversion therapy in HCC and develop consensus statements to guide clinical practice.Evidence review:Many research centers in China have accumulated significant experience implementing HCC conversion therapy.Preliminary findings and data have shown that conversion therapy represents an important strategy to maximize the survival of selected patients with intermediate stage to advanced HCC;however,there are still many urgent clinical and scientific challenges for this therapeutic strategy and its related fields.In order to summarize and learn from past experience and review current challenges,the Chinese Expert Consensus on Conversion Therapy for Hepatocellular Carcinoma(2021 Edition)was developed based on a review of preliminary experience and clinical data from Chinese and non-Chinese studies in this field and combined with recommendations for clinical practice.Sixteen consensus statements on the implementation of conversion therapy for HCC were developed.The statements generated in this review are based on a review of clinical evidence and real clinical experience and will help guide future progress in conversion therapy for patients with HCC. | Hui-Chuan Sun Jian Zhou Zheng Wang Xiufeng Liu Qing Xie Weidong Jia Ming Zhao Xinyu Bi Gong Li Xueli Bai Yuan Ji Li Xu Xiao-Dong Zhu Dousheng Bai Yajin Chen Yongjun Chen Chaoliu Dai Rongping Guo Wenzhi Guo Chunyi Hao Tao Huang Zhiyong Huang Deyu Li Gang Li Tao Li Xiangcheng Li Guangming Li Xiao Liang Jingfeng Liu Fubao Liu Shichun Lu Zheng Lu Weifu Lv Yilei Mao Guoliang Shao Yinghong Shi Tianqiang Song Guang Tan Yunqiang Tang Kaishan Tao Chidan Wan Guangyi Wang Lu Wang Shunxiang Wang Tianfu Wen Baocai Xing Bangde Xiang Sheng Yan Dinghua Yang Guowen Yin Tao Yin Zhenyu Yin Zhengping Yu Bixiang Zhang Jialin Zhang Shuijun Zhang Ti Zhang Yamin Zhang Yubao Zhang Aibin Zhang Haitao Zhao Ledu Zhou Wu Zhang Zhenyu Zhu Shukui Qin Feng Shen Xiujun Cai Gaojun Teng Jianqiang Cai Minshan Chen Qiang Li Lianxin Liu Weilin Wang Tingbo Liang Jiahong Dong Xiaoping Chen Xuehao Wang Shusen Zheng Jia Fan 无 | 2022 | Hepatobiliary Surgery and Nutrition2022，11，2： | 4 |
| 14 | Numerical Simulation of Solidification Microstructure and Effects of Phase-field Parameters on Grain Growth Morphologies显示文摘By a simple phase field model, a series of numerical simulations of solidification microstructure was performed to show a rich variety of dendritic patterns. At the same time, the relation between the morphology of grain growth and some parameters including the strength of anisotropy, dimensionless latent heat and the size of initial solid zone was studied. It is for the first time that patterns of grain growth were associated with the size of initial solid zone, which is an interesting issue. The possible reason for this may be that variation in the size of initial solid zone may bring about fluctuation of the interface energy, making the interface unstable. | Jingfeng LIU+, Ruixiang LIU and Liliang CHEN Research Studio of Solidification Simulation, College of Materials Science and Engineering, Huazhong University of Science and Technology, Wuhan 430074, China | 2005 | Journal of Materials Science & Technology2005，21，6： | 4 |
| 15 | Effects of sericin on heme oxygenase-1 expression in the hippocampus and cerebral cortex of type 2 diabetes mellitus rats显示文摘Previous studies have demonstrated that sericin effectively reduces blood glucose,and protects islet cells,as well as the gonads and kidneys.However,whether sericin improves diabetes mellitus-induced structural and functional problems in the central nervous system remains poorly understood.Rat models of type 2 diabetes mellitus were established by intraperitoneal injection of streptozotocin.The present study observed histological changes in the hippocampus and cerebral cortex,as well as heme oxygenase-1 expression,and explored sericin effects on the central nervous system in diabetic rats.Pathological damage to neural cells in the rat hippocampus and cerebral cortex was relieved following intragastric administration of sericin at a dose of 2.4 g/kg for 35 consecutive days.Heme oxygenase-1 protein and mRNA expressions were decreased in the hippocampus and cerebral cortex of diabetes mellitus rats after sericin treatment.The results suggest that sericin plays a protective effect on the nervous system by decreasing the high expression of heme oxygenase-1 following diabetes mellitus. | Zhihong Chen Yaqiang He Wenliang Fu Jingfeng Xue | 2011 | Neural Regeneration Research2011，6，6： | 4 |
| 16 | Development of metal-organic framework(MOF) decorated graphene oxide/Mg Al-layered double hydroxide coating via microstructural optimization for anti-corrosion micro-arc oxidation coatings of magnesium alloy显示文摘An efcient and simple in-situ growth strategy has been discovered for the preparation of highly reproducible and continuous symbiotic ZIF-8-based anticorrosion coating by using graphene oxide(GO)/Mg AlNO3layered double hydroxides(G/LDHs) buffer layer as a new type of connecting carrier based on micro-arc oxide(MAO) coating of AZ31 magnesium alloy. The components of ZIF-8 were adsorbed and bounded to the surface of the G/LDHs buffer layer-modified substrates to promote the nucleation of ZIF-8,thus growing a phase-pure, uniform, and good symbiosis ZIF-8 membrane. ZIF-8 particles with different growth times compensate for the grain boundary defects of the G/LDHs coating precursor buffer layer to different degrees. The prepared ZIF-8-based coating has excellent stability and corrosion resistance. The results demonstrate that the G/LDHs buffer layer provides a new channel for the MOF-modified MAO substrate of AZ31 magnesium alloy. It also proves that it is feasible to build high-performance anticorrosive coatings with MOF materials. | Yanning Chen Liang Wu Wenhui Yao Jiahao Wu Jianpeng Xiang Xiaowei Dai Tao Wu Yuan Yuan Jingfeng Wang Bin Jiang Fusheng Pan | 2022 | Journal of Materials Science & Technology2022，，35： | 3 |
| 17 | A CRISPR/Cas12a-empowered surface plasmon resonance platform for rapid and specific diagnosis of the Omicron variant of SARS-CoV-2显示文摘The outbreak of the COVID-19 pandemic was partially due to the challenge of identifying asymptomatic and presymptomatic carriers of the virus,and thus highlights a strong motivation for diagnostics with high sensitivity that can be rapidly deployed.On the other hand,several concerning SARS-CoV-2 variants,including Omicron,are required to be identified as soon as the samples are identified as‘positive’.Unfortunately,a traditional PCR test does not allow their specific identification.Herein,for the first time,we have developed MOPCS(Methodologies of Photonic CRISPR Sensing),which combines an optical sensing technology-surface plasmon resonance(SPR)with the‘gene scissors’clustered regularly interspaced short palindromic repeat(CRISPR)technique to achieve both high sensitivity and specificity when it comes to measurement of viral variants.MOPCS is a low-cost,CRISPR/Cas12a-systemempowered SPR gene-detecting platform that can analyze viral RNA,without the need for amplification,within 38 min from sample input to results output,and achieve a limit of detection of 15 fM.MOPCS achieves a highly sensitive analysis of SARS-CoV-2,and mutations appear in variants B.1.617.2(Delta),B.1.1.529(Omicron)and BA.1(a subtype of Omicron).This platform was also used to analyze some recently collected patient samples from a local outbreak in China,identified by the Centers for Disease Control and Prevention.This innovative CRISPR-empowered SPR platform will further contribute to the fast,sensitive and accurate detection of target nucleic acid sequences with single-base mutations. | Zhi Chen Jingfeng Li Tianzhong Li Taojian Fan Changle Meng Chaozhou Li Jianlong Kang Luxiao Chai Yabin Hao Yuxuan Tang Omar AAl-Hartomy Swelm Wageh Abdullah GAl-Sehemi Zhiguang Luo Jiangtian Yu Yonghong Shao Defa Li Shuai Feng William J.Liu Yaqing He Xiaopeng Ma Zhongjian Xie Han Zhang | 2022 | National Science Review2022，9，8： | 3 |
| 18 | Profiling of hepatocellular carcinoma neoantigens reveals immune microenvironment and clonal evolution related patterns显示文摘Objective: Neoantigens derived from tumor-specific genomic alterations have demonstrated great potential for immunotherapeutic interventions in cancers. However, the comprehensive profile of hepatocellular carcinoma(HCC) neoantigens and their complex interplay with immune microenvironment and tumor evolution have not been fully addressed.Methods: Here we integrated whole exome sequencing data, transcriptome sequencing data and clinical information of 72 primary HCC patients to characterize the HCC neoantigen profile, and systematically explored its interactions with tumor clonal evolution, driver mutations and immune microenvironments.Results: We observed that higher somatic mutation/neoantigen load was associated with better clinical outcomes and HCC patients could be further divided into two subgroups with distinct prognosis based on their neoantigen expression patterns. HCC subgroup with neoantigen expression probability high(NEP-H) showed more aggressive pathologic features including increased incidence of tumor thrombus(P=0.038), higher recurrence rate(P=0.029),more inclined to lack tumor capsule(P=0.026) and with more microsatellite instability sites(P=0.006). In addition,NEP-H subgroup was also characterized by higher chance to be involved in tumor clonal evolution [odds ratio(OR)=46.7, P<0.001]. Gene set enrichment analysis revealed that upregulation of MYC and its targets could suppress immune responses, leading to elevated neoantigen expression proportion in tumor cells. Furthermore, we discovered an immune escape mechanism that tumors could become more inconspicuous by evolving subclones with less immunogenicity. We observed that smaller clonal mutation clusters with higher immunogenicity in tumor were more likely to involve in clonal evolution. Based on identified neoantigen profiles, we also discovered series of neoantigenic hotspot genes, which could serve as potential actionable targets in future.Conclusions: Our results revealed the landscape of HCC neoantigens and discovered two clinically relevant subgroups with distinct neoantigen expression patterns, suggesting the neoantigen expression should be fully considered in future immunotherapeutic interventions. | Zhenli Li Geng Chen Zhixiong Cai Xiuqing Dong Lei He Liman Qiu Yongyi Zeng Xiaolong Liu Jingfeng Liu | 2021 | Chinese Journal of Cancer Research2021，33，3： | 3 |
| 19 | Big Data Analytics in Healthcare: Data-Driven Methods for Typical Treatment Pattern Mining显示文摘A huge volume of digitized clinical data is generated and accumulated rapidly since the widespread adoption of Electronic Medical Records (EMRs).These big data in healthcare hold the promise of propelling healthcare evolving from a proficiency-based art to a data-driven science,from a reactive mode to a proactive mode,from one-size-fits-all medicine to personalized medicine.This paper first discusses the research background-big data analytics in healthcare,the research framework of big data analytics in healthcare,analysis of medical process,and the literature summary of treatment pattern mining.Then the challenges for data-driven typical treatment pattern mining are highlighted,including similarity measure between treatment records,typical treatment pattern extraction,evaluation and recommendation,when considering the rich temporal and heterogeneous medical information in EMRs.Furthermore,three categories of typical treatment patterns are mined from doctor order content,duration,and sequence view respectively,which can provide a data-driven guideline to achieve the '5R' goal for rational drug use and clinical pathways. | Chonghui Guo Jingfeng Chen | 2019 | Journal of Systems Science and Systems Engineering2019，28，6： | 3 |
| 20 | Bioinformatic analysis for potential biological processes and key targets of heart failure-related stroke显示文摘This study aimed to uncover underlying mechanisms and promising intervention targets of heart failure(HF)-related stroke.HF-related dataset GSE42955 and stroke-related dataset GSE58294 were obtained from the Gene Expression Omnibus(GEO)database.Weighted gene co-expression network analysis(WGCNA)was conducted to identify key modules and hub genes.Gene Ontology(GO)and pathway enrichment analyses were performed on genes in the key modules.Genes in HF-and stroke-related key modules were intersected to obtain common genes for HF-related stroke,which were further intersected with hub genes of stroke-related key modules to obtain key genes in HF-related stroke.Key genes were functionally annotated through GO in the Reactome and Cytoscape databases.Finally,key genes were validated in these two datasets and other datasets.HF-and stroke-related datasets each identified two key modules.Functional enrichment analysis indicated that protein ubiquitination,Wnt signaling,and exosomes were involved in both HF-and stroke-related key modules.Additionally,ten hub genes were identified in stroke-related key modules and 155 genes were identified as common genes in HF-related stroke.OTU deubiquitinase with linear linkage specificity(OTULIN)and nuclear factor interleukin 3-regulated(NFIL3)were determined to be the key genes in HF-related stroke.Through functional annotation,OTULIN was involved in protein ubiquitination and Wnt signaling,and NFIL3 was involved in DNA binding and transcription.Importantly,OTULIN and NFIL3 were also validated to be differentially expressed in all HF and stroke groups.Protein ubiquitination,Wnt signaling,and exosomes were involved in HF-related stroke.OTULIN and NFIL3 may play a key role in HF-related stroke through regulating these processes,and thus serve as promising intervention targets. | Chiyu LIU Sixu CHEN Haifeng ZHANG Yangxin CHEN Qingyuan GAO Zhiteng CHEN Zhaoyu LIU Jingfeng WANG | 2021 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2021，22，9： | 3 |
您所在的机构暂未开通此部分的功能，请联系管理员