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| 1 | Evaluation of the Xpert MTB/RIF assay for diagnosis of tuberculosis and rifampin resistance in county-level laboratories in Hunan province, China显示文摘 | Hu Peilei Bai Liqiong Liu Fengping Ou Xichao Zhang Zhiying Yi Songlin Chen Zhongnan Gong Daofang Liu Binbin Guo Jingwei Tan Yunhong | 2014 | Chinese Medical Journal2014，，21： | 24 |
| 2 | Molecular cloning,characterization and expression analysis of Sox9a and Foxl2 genes in half-smooth tongue sole(Cynoglossussemilaevis)显示文摘Two transcription factors,Sox9a and Foxl2 were cloned from half-smooth tongue sole (Cynoglossus semilaevis).Sox9a is a new duplication of C.semilaevis Sox9 gene.The complete cDNA of Sox9a gene was 1 842 bp long coding for 487 amino acids and Foxl2 gene was 1 817 bp coding for 308 amino acids.Sox9a was expressed higher in male brain,pituitary and gonad and Foxl2 were higher in female brain,pituitary and gonad.The expression of Sox9a gene in gonads of neo-males was higher than that of normal females.Sox9a and Foxl2 were expressed higher in gastrula stage than in other stages.In the period of sex differentiation,the expression of Sox9a was first going up and then going down and Foxl2 was higher expressed at 37 dph.The highest expressions of Sox9a and Foxl2 genes occurred in nine-month and 12-month old gonad tissues,respectively.Sox9a gene was considered to have inevitable links with sex reversal,sex differentiation and cell differentiation of embryos and formation of spermatogenic cells.Foxl2 was considered to play a role in sex differentiation,cell differentiation of embryos but not to be necessary for sex determination and sex reversal. | DONG Xiaoli CHEN Songlin JI Xiangshan SHAO Changwei | 2011 | Acta Oceanologica Sinica2011，30，1： | 20 |
| 3 | Estimates of genetic and phenotypic parameters for weight and length in Paralichthys olivaceus(Temminck et Schlegel)显示文摘The complete diallel cross design was employed to estimate the genetic parameters of the growth of Japanese flounder (Paralichthys olivaceus). A total of 60 full-sib families were cultivated and two growth-related traits, body weight (BW) and body length (BL), were examined at average 2, 3 and 8 months of age respectively, with 1 800 individuals measured in each age group (30 per family). Based on the additive-dominance-maternal-effect genetic analysis model, the restricted maximum likelihood approach was used to estimate various (co)variance components. The results showed that narrow-sense heritability estimates of BW and BL were respectively: 0.29 ± 0.01 and 0.22 ± 0.02 at 2 months of age, 0.32 ± 0.02 and 0.30 ± 0.04 at 3 months of age, 0.48 ± 0.04 and 0.40 ± 0.05 at 8 months of age; broad-sense heritability estimates were respectively: 0.44 ± 0.02 and 0.54 ± 0.04 at 2 months of age, 0.35 ± 0.01 and 0.36 ± 0.03 at 3 months of age, 0.49 ± 0.03 and 0.45 ± 0.04. All heritabilities were statistically significant (P <0.05). Additive genetic correlations between BW and BL at 2, 3 and 8 months of age were consistently positive and highly significant (P <0.01): 0.93 ± 0.02, 0.95 ± 0.03 and 0.92 ± 0.03 respectively. Maternal effect was significant (P <0.05) only at 2 months of age, and was not detected at 3 and 8 months of age. According to the heritability estimates, the mass selection strategy should be efficient for the breeding of Japanese flounder. | TIAN Yongsheng XU Tianjun LIANG You CHEN Songlin | 2011 | Acta Oceanologica Sinica2011，30，6： | 13 |
| 4 | Microstructure, porosity and mechanical properties of selective laser melted AlSi10Mg显示文摘Finite element modeling(FEM),microscopy,X-ray computed tomography(CT)and mechanical property tests were used to study the microstructure,porosity and mechanical properties of an AlSi10Mg alloy produced by selective laser melting(SLM).The simulation of the melt pool and thermal history under different energy densities produced an optimized result with an energy density of 44.5 J·mm-3.The high cooling rate during the SLM process significantly refined the previous a-Al dendrites.The growth direction of the network-like Al-Si eutectic structure at different orientations confirmed the anisotropic nature of the microstructure.Furthermore,the microhardness,tensile testing and fracture analysis results proved that there were no obvious distinctions in the strength between the transverse and longitudinal directions,and that the ductility was anisotropic,possibly due to the shape and distribution of the pores.The pores measured by X-ray CT at different energy densities confirmed that the sphericity of the pores was inversely related to pores volumes.With optimized processing conditions,the porosity of the selective laser melted sample decreased leading to the improved fabricated fuel system component via SLM. | Jing CHEN Wei HOU Xiuzhuan WANG Songlin CHU Zhiyi YANG | 2020 | Chinese Journal of Aeronautics2020，33，7： | 9 |
| 5 | Stability analysis of networked control systems with time-varying sampling periods显示文摘In this paper, we present an interval model of networked control systems with time-varying sampling periods and time-varying network-induced delays and discuss the problem of stability of networked control systems using Lyapunov stability theory. A sufficient stability condition is obtained by solving a set of linear matrix inequalities. In the end, the illustrative example demonstrates the correctness and effectiveness of the proposed approach. | Fuchun LIU Yu YAO FenghuaHE Songlin CHEN | 2008 | 控制理论与应用(英文版)2008，6，1： | 8 |
| 6 | Zircon Hf Isotopes and Geochemistry of the Early Paleoproterozoic High-Sr Low-Y Quartz-Diorite in the Quanji Massif,NW China:Crustal Growth and Tectonic Implications显示文摘The metamorphosed Early Paleoproterozoic granitoids in the Quanji massif,Northwestern China provide constraints for relationship between the Tarim Craton and North China Craton.Among granitoids batholiths,rocks of the Mohe quartz-diorite show typically adakitic geochemical characteristics,with medium K2O/Na2O ratios(0.56–1.17)and high Sr(519–619 ppm)low Y(9.37–20.40 ppm)and Yb(0.97–1.77 ppm)concentrations.The rocks haveεNd(t)values between+2.4and+4.4 and depleted mantle Nd model ages of 2.43–2.59 Ga.The magmatic zircons have positiveεHf(t)values ranging from+0.40 to+7.60 and depleted mantle Hf model ages of 2.43–2.70 Ga,with major peaks at^2.54 and^2.65 Ga.The geochemical and Nd-Hf isotopic characteristics indicate that the Mohe quartz-dioritic rocks might be formed by partial melting of high-pressure metamorphosed juvenile crustal rocks in post-orogenic extensional regime in the Early Paleoproterozoic.It suggests that important crustal growth occurred in the Quanji massif and the Tarim Craton at^2.4 and 2.5–2.7 Ga.The Quanji massif and Tarim Craton might share a similar crustal evolution history with the North China Craton in the Neoarchean. | Songlin Gong Nengsong Chen Hongyan Geng Min Sun Lu Zhang Qinyan Wang | 2014 | Journal of Earth Science2014，25，1： | 8 |
| 7 | Circular RNA circStag1 promotes bone regeneration by interacting with HuR显示文摘Postmenopausal osteoporosis is a common bone metabolic disorder characterized by deterioration of the bone microarchitecture,leading to an increased risk of fractures.Recently,circular RNAs(circ RNAs)have been demonstrated to play pivotal roles in regulating bone metabolism.However,the underlying functions of circ RNAs in bone metabolism in postmenopausal osteoporosis remain obscure.Here,we report that circ Stag1 is a critical osteoporosis-related circ RNA that shows significantly downregulated expression in osteoporotic bone marrow mesenchymal stem cells(BMSCs)and clinical bone tissue samples from patients with osteoporosis.Overexpression of circ Stag1 significantly promoted the osteogenic capability of BMSCs.Mechanistically,we found that circ Stag1 interacts with human antigen R(Hu R),an RNA-binding protein,and promotes the translocation of Hu R into the cytoplasm.A high cytoplasmic level of Hu R led to the activation of the Wnt signaling pathway by stabilizing and enhancing low-density lipoprotein receptor-related protein 5/6(Lrp5/6)andβ-catenin expression,thereby stimulating the osteogenic differentiation of BMSCs.Furthermore,overexpression of circ Stag1 in vivo by circ Stag1-loaded adeno-associated virus(circ Stag1-AAV)promoted new bone formation,thereby preventing bone loss in ovariectomized rats.Collectively,we show that circ Stag1 plays a pivotal role in promoting the regeneration of bone tissue via Hu R/Wnt signaling,which may provide new strategies to prevent bone metabolic disorders such as postmenopausal osteoporosis. | Gaoyang Chen Canling Long Shang Wang Zhenmin Wang Xin Chen Wanze Tang Xiaoqin He Zhiteng Bao Baoyu Tan Jin Zhao Yongheng Xie Zhizhong Li Dazhi Yang Guozhi Xiao Songlin Peng | 2022 | Bone Research2022，10，3： | 7 |
| 8 | Development and characterization of a cell line from the embryos of half smooth tongue sole (Cynoglossus semilaevis)显示文摘A new cell line,CSEC,has been successfully established from embryos at gastrula stage of a cultured marine fish,half smooth tongue sole(Cynoglossus semilaevis).CSEC cells grow actively and stably more than 50 passages for over 200 d in DMEM medium supplemented with 15% FBS(fetal bovine serum),2.5 ng/cm 3 bFGF(basic fibroblast growth factor),1 ng/cm 3 LIF(leukemia inhibitory factor) and 50 mmol/dm 3 2-ME(2-mecaptoethanol).The cells grew well in the temperature range of 24-30 ℃ and the optimal growth temperature was 24℃.FBS and bFGF concentrations are the two key components for CSEC cells proliferation.Chromosome analysis reveals that CSEC cells have a normal diploid karyotype with 2n=42t.The significant fluorescent signals were observed in CSEC after transfection with the GFP reporter gene,suggesting that the CSEC cell line can be used as a useful tool for transgenic and genetic manipulation studies.CSEC cells showed the cytopathic effect(CPE) after infection with lymphosystis disease virus(LCDV) in 2 d.Moreover,the LCDV particles can be observed in the cytoplasm of virus-infected cells by electron microscopy.It suggests that CSEC could be potentially used for the study of aquatic virus. | SHA Zhenxia REN Guocheng WANG Xianli WANG Na CHEN Songlin | 2010 | Acta Oceanologica Sinica2010，29，2： | 7 |
| 9 | Progress on design and related R&D activities for the water-cooled breeder blanket for CFETR显示文摘The water-cooled ceramic breeder (WCCB) blanket is one of the blanket candidates for Chinese fusion engineering testing reactor (CFETR) and is being developed at the Institute of Plasma Physics, Chinese Academy of Sciences (ASIPP). This paper reviews design and evolution of the WCCB blanket for CFETR, and presents a new WCCB blanket design according to the latest CFETR core parameters (major and minor radii are R = 7.2 m and a = 2.2 m, respectively) and missions. This new design is expected to satisfy multiple CFETR operation modes of 0.2, 0.5, 1.0, and 1.5 GW fusion power and achieve tritium self-sufficiency. The feasibility of the updated blanket design is evaluated from the aspects of neutronics and thermo-hydraulics. Furthermore, the research and development (R&D) activities supporting to the WCCB blanket for CFETR are reported, including the design code, the water loop experiments, the pebble bed modeling and experiments, and the components fabrication technology. | Songlin Liu Xiaoman Cheng Xuebin Ma Lei Chen Kecheng Jiang Xia Li Hui Bao Jichao Wang Wanjing Wang Changhong Peng Peng Lu Min Li Kai Huang | 2019 | Theoretical & Applied Mechanics Letters2019，9，3： | 6 |
| 10 | Adjuvant radiotherapy in central hepatocellular carcinoma after narrow-margin hepatectomy:A 10-year real-world evidence显示文摘Objective:A prospective randomized control study investigated the feasibility and efficacy of adjuvant radiotherapy on patients with central hepatocellular carcinoma(HCC)after narrow-margin hepatectomy(<1 cm).This study presents an updated 10-year real-world evidence to further characterize the role of adjuvant radiotherapy.Methods:Patients with central HCC after narrow-margin hepatectomy(<1 cm)were prospectively assigned to adjuvant radiotherapy group and control group.Patients'outcome,adverse events,long-term recurrence and survival rates were investigated.Results:The 1-,5-,and 10-year recurrence-free survival(RFS)rates were 81.0%,43.9%,and 38.7%,respectively in adjuvant radiotherapy group and 71.7%,35.8%,and 24.2%,respectively in control group(log-rank test,P=0.09).The 1-,5-,and 10-year overall survival(OS)rates were 96.6%,54.7%,and 42.8%,respectively in adjuvant radiotherapy group and 90.2%,55.1%,and 30.0%,respectively in control group(log-rank test,P=0.20).The 1-,5-,and 10-year RFS rates for patients with small HCC(≤5 cm)were 91.1%,51.6%,and 48.4%,respectively in adjuvant radiotherapy group and 80.0%,36.6%,and 26.6%,respectively in control group(log-rank test,P=0.03).Landmark analysis demonstrated that patients with small HCC in adjuvant radiotherapy group had a significantly improved OS in second five years after treatment in comparison to patients in control group(log-rank test,P=0.05).Conclusions:Our updated results showed a sustained clinical benefit on reducing recurrence,improving longterm survival for small central HCC by adjuvant radiotherapy after narrow-margin hepatectomy.Long-term survival data also indicated that hepatectomy is an optimal treatment for selected patients with central HCC. | Weiqi Rong Weibo Yu Liming Wang Fan Wu Kai Zhang Bo Chen Chengli Miao Liguo Liu Songlin An Changcheng Tao Weihu Wang Jianxiong Wu | 2020 | Chinese Journal of Cancer Research2020，32，5： | 6 |
| 11 | Mode splitting transmission effect of surface wave excitation through a metal hole array显示文摘The resonant frequencies of the excited surface waves on a metal hole array with respect to the incident angle were studied in the terahertz region.The experimental and theoretical results demonstrate that the resonant peak of surface wave excitation splits into two when transmitted through a metal hole array off-normally.The high-order mode with resonant frequency above the cutoff frequency fc(plasma frequency effect)has a shorter attenuation length than that of the low-order mode whose resonant frequency is below fc.The reason is that the high-order mode is a coupled mode consisting of surface wave and hole modes,while the low-order mode is just an excited surface wave(which can be considered as the spoof surface plasmons).Our investigation may open a door to distinguish the spoof surface plasmons and the coupled modes of surface waves and hole modes. | Lin Chen Yiming Zhu Xiaofei Zang Bin Cai Zhou Li Le Xie Songlin Zhuang | 2013 | Light(Science & Applications)2013，2，1： | 6 |
| 12 | Effect of reinforcing kidney-essence, removing phlegm, and promoting mental therapy on treating Alzheimer's disease显示文摘OBJECTIVE: To explore the mechanism of reinforcing kidney-essence, removing phlegm, and promoting mental therapy in treating Alzheimer's disease (AD). METHODS: Sixty patients with AD in Wuhan No.1 Hospital of Traditional Chinese Medicine for Geriatrics from May 2009 to April 2011 were randomly divided into two groups, with 30 in each group. Patients in Bushenhuatanyizhi group (BHY group) took BHY instant granules (6 g, twice per day). Patients in the control group took piracetam (0.8 g, 3 times per day). There were twelve weeks in a course. Changes in the mini-mental state examination (MMSE) score and Activity of Daily Living Scale (ADL) score were analyzed before and after treatment. RESULTS: MMSE scores in the two groups in-creased and ADL scores decreased after treatment, compared with those before treatment (both P< 0.05).The total effective rate was 86.67% in the BHY group (10 very effective, 13 effective, 7 non-responsive). The control group was 57.69% (5 very effective, 16 effective, 9 non-responsive) (P<0.05). Superoxide dismutase levels were increased, lipid peroxide and triglyceride levels decreased after treatment in the BHY group as compared with the control group. CONCLUSION: Reinforcing kidney-essence, removing phlegm, and promoting mental therapy can improve cognitive function and daily life of AD patients. The mechanism of the therapy might be related to improving blood fat, scavenging free radicals, and inhibiting lipid peroxides. | Ping Liu Mingwang Kong Songlin Liu Gang Chen Ping Wang | 2013 | Journal of Traditional Chinese Medicine2013，33，4： | 5 |
| 13 | Genetic Analysis of Gynogenetic and Common Populations of Verasper moseri Using SSR Markers显示文摘The genetic structure and variation of the artificial meio- gy- nogenetic population and common population of barfin flounder (Verasper moseri) were analyzed using eight microsatellite markers. A total of 29 alleles were detected, of which 23 alleles were in the artificial gynogenetic population while 29 alleles were in the control group. The average ob- served heterozygosity (HO) of eight loci in the control group (0.526 8) was several times higher than that (0.185 8) in the gynogenetic population. The results indicate that the genetic diversity of the control group was much higher than that of the gynogenetic population of barfin flounder (Verasper moseri). Most loci significantly deviated from Hardy-Weinberg equilib- rium (HWE) after Bonferroni correction (p <0.005 56) in the gynoge- netic population, while four loci deviated from HWE in the control group. The coefficient of gene differentiation (GST) was 0.131 0, and the genetic distance was 0.171 8 between the two populations, suggesting a signifi- cant genetic differentiation between the two populations. | MA Hongyu YANG Jingfeng SU Pengzhi CHEN Songlin | 2009 | Wuhan University Journal of Natural Sciences2009，14，3： | 5 |
| 14 | MHC polymorphism and disease-resistance to Edwardsiella tarda in six turbot(Scophthalmus maximus) families显示文摘This study examined genetic variation in the major histocompatibility complex(MHC) Class II B gene in turbot(Scophthalmus maximus) by virulent bacterial pathogen challenge.One hundred fry from each of six families were infected with Edwardsiella tarda by intraperitoneal injection.Family mortality ranged from 28.0% to 83.3%.Complete exon 2 and intron 1 sequences of MHC Class II B genes were amplified from five survivor and five non-survivor individuals per family using the clone-sequence method.Thirty-seven sequences from 60 individuals revealed 37 different alleles,25 of which were unique to this study.The 25 unique alleles belonged to 16 major allele types.Nine alleles were used to examine the association between alleles and resistance/susceptibility to disease.Five alleles were present in an individual,suggesting a minimum of three loci or copies of the turbot MHC Class II B gene.The rate of non-synonymous substitution(d N) was 2.30 and 1.58 times higher than synonymous substitution(d S) in the peptide-binding regions(PBR) and non-PBR in whole families,respectively,which suggested balancing selection on exon 2 of the MHC Class II B gene in turbot.One allele,Scma-DBB1*02,was significantly more prevalent in survivor stock than in non-survivor stock(P=0.001).Therefore,this allele might be associated with resistance to bacteria.A second allele,Scma-DBB1*10,was significantly more prevalent in non-survivor stock(P=0.021),and is likely associated with susceptibility to bacteria. | DU Min CHEN SongLin LIU YanHong NIU BaoZhen YANG JingFeng ZHANG Bo | 2012 | Chinese Science Bulletin2012，57，25： | 5 |
| 15 | Cloning,characterization of two female-specific AFLP markers and development of PCR-based sex identification method for the half-smooth tongue sole Cynoglossus semilaevis显示文摘Two female-specific AFLP(amplified fragment length polymorphism)markers(named CseF464 and CseF136)were isolated by using one selective primer combination(E-AGC/M-CTG)from the genomic DNA of 20 females and 20 males of the half-smooth tongue sole Cynoglossus semilaevis.Both the markers were re-amplified,recovered from the agarose gels,cloned and sequenced.Bioinformatics analysis indicated that the length of the two markers were 468 bp and 134 bp,respectively,and the sequences showed no similarity to each other,as well as to the known sequences deposited in the GenBank database using BLASTn.Two pairs of SCAR(sequence characterized amplified regions)primers were designed based on the sequences of the two female-specific markers.Furthermore,PCR-based genetic sex identification method was developed in Cynoglossus semilaevis.A specific fragment was amplified in all females but not in any males by using these SCAR primers on the initial 20 female and 20 male individuals of Cynoglossus semilaevis.The feasibility of the two SCAR primer pairs was confirmed in additional 100 individuals(50 females and 50 males).This allowed for reliable,rapid molecular identification of genetic sex of the species,genetic mapping on the sex chromosomes and better understanding of the sex determination and sex differentiation in the half-smooth tongue sole. | Hongyu MA Songlin CHEN Jing LI Jin-Zhen BI Tianjun XU | 2009 | Current Zoology2009，55，4： | 4 |
| 16 | Photoinduced large polaron transport and dynamics in organic-inorganic hybrid lead halide perovskite with terahertz probes显示文摘Organic-inorganic hybrid metal halide perovskites(MHPs)have attracted tremendous attention for optoelectronic applications.The long photocarrier lifetime and moderate carrier mobility have been proposed as results of the large polaron formation in MHPs.However,it is challenging to measure the effective mass and carrier scattering parameters of the photogenerated large polarons in the ultrafast carrier recombination dynamics.Here,we show,in a one-step spectroscopic method,that the optical-pump and terahertz-electromagnetic probe(OPTP)technique allows us to access the nature of interplay of photoexcited unbound charge carriers and optical phonons in polycrystalline CH_(3)NH_(3)PbI_(3)(MAPbI_(3))of about 10μm grain size.Firstly,we demonstrate a direct spectral evidence of the large polarons in polycrystalline MAPbI_(3).Using the Drude-Smith-Lorentz model along with the Frӧhlich-type electron-phonon(e-ph)coupling,we determine the effective mass and scattering parameters of photogenerated polaronic carriers.We discover that the resulting moderate polaronic carrier mobility is mainly influenced by the enhanced carrier scattering,rather than the polaron mass enhancement.While,the formation of large polarons in MAPbI_(3)polycrystalline grains results in a long charge carrier lifetime at room temperature.Our results provide crucial information about the photo-physics of MAPbI3 and are indispensable for optoelectronic device development with better performance. | Zuanming Jin Yan Peng Yuqing Fang Zhijiang Ye Zhiyuan Fan Zhilin Liu Xichang Bao Heng Gao Wei Ren Jing Wu Guohong Ma Qianli Chen Chao Zhang Alexey VBalakin Alexander PShkurinov Yiming Zhu Songlin Zhuang | 2022 | Light(Science & Applications)2022，11，8： | 4 |
| 17 | Alzheimer's disease treated with combined therapy based on nourishing marrow and reinforcing Qi显示文摘OBJECTIVE:To study the efficacy and safety of combined Traditional Chinese Medicine(TCM) therapy based on nourishing marrow to improve intellect and reinforcing Qi to activate bloodon mild to moderate Alzheimer's disease(AD).METHODS:Sixty-six patients with AD,whoseMini-Mental State Examination(MMSE) score were from 10-24,were randomized equally into an intervention group and a control group.The control group was given Aricept(5 mg,once daily).The intervention group was further divided into Yang-Qi deficiency(n = 18) and of Yin-Qi deficiency(n = 15)subgroups.Patients in the Yang-Qi deficiency group were intravenously administered Shenfu injection,60 mL,and deproteinized calf blood injection(DCBl),1.2 g,once daily.The Yin-Qi deficiency group was given Shenmai injection,60 mL,and DCBl,1.2 g,once daily.Each course lasted 21 days.RESULTS:Compared with the control group and with pre-treatment in the same group,MMSE,clinical dementia rating,and activities of daily living scale scores in the intervention group were significantly improved(all P<0.05).These metrics mildly improved in the control group compared with before treatment(P>0.05).No adverse effects were observed in any group during treatment.CONCLUSION:We found that combined TCM therapy is effective and safe for managing mild to moderate AD. | Chen Songlin Yao Xiaoli Liang Yinying Mei Weiyi Liu Xiaoyun Zhang Changran | 2015 | Journal of Traditional Chinese Medicine2015，35，3： | 4 |
| 18 | Growth and metal uptake of energy sugarcane (Saccharum spp.) in different metal mine tailings with soil amendments显示文摘A pot experiment was conducted to investigate the feasibility of growing energy sugarcane(Saccharum spp.) in three different metal mine tailings(Cu, Sn and Pb/Zn tailings) amended with uncontaminated soil at different mixing ratios. The results indicated that sugarcane was highly tolerant to tailing environments. Amendments of 20% soil to Sn tailings and 30% soil to Cu tailings could increase the biomass of cane-stem for use as the raw material for bioethanol production. Heavy metals were mostly retained in roots, which indicated that sugarcane was useful for the stabilization of the tailings. Bagasse and juice, as the most valuable parts to produce bioethanol, only accounted for 0.6%– 3% and 0.6%–7% of the total metal content. Our study supported the potential use of sugarcane for tailing phytostabilization and bioenergy production. | Xin Zhang Yongguan Zhu Yuebin Zhang Yunxia Liu Shaochun Liu Jiawen Guo Rudan Li Songlin Wu Baodong Chen | 2014 | Journal of Environmental Sciences2014，26，5： | 4 |
| 19 | Analysis of SSRs Derived from an EST Library of Half-Smooth Tongue Sole Cynoglossus semilaevis显示文摘A complementary DNA(cDNA) library was constructed from half-smooth tongue sole spleen. A long-read expressed sequence tag(EST) database was generated,containing 3100 cDNA clones,of which 220 clones were fully sequenced. A total of 1060 non-redundant simple sequence repeats(SSRs) were obtained from the cDNA library. An average of 5 kb sequence generates 1 SSR in the half-smooth tongue sole spleen cDNA library. The proportion of the SSR unit size was different in the cDNA library. The monomeric repeats(51.4%) are the most abundant class of SSR in the dataset. The dimeric,trimeric,tetrameric and hexameric re-peats are represented in decreasing proportions of 27.2%,16.0%,2.8% and 1.9%,respectively. The frequency of pentameric repeats was observed the least(only 0.7%) . Most of the monomeric and dimeric repeats are distributed in 3' and 5' un-translation region. If translation regions are considered merely,trimeric repeats are the highest,accounting for 57% of the total microsatellites. | LIU Yunguo BAO Baolong CHEN Songlin SUN Xiuqin | 2008 | Journal of Ocean University of China2008，7，3： | 4 |
| 20 | Establishment and Characterization of a New Cell Line from the Kidney of Spotted Halibut Verasper variegates显示文摘A cell line,SHK,was derived from the kidney of spotted halibut Verasper variegates.The cell line was subculturedmore than 40 passages in minimum essential medium(MEM)supplemented with fetal bovine serum(FBS)and 10 ng ml-1basic fi-broblast growth factor(bFGF).Cell morphology from primary culture and subculture was observed continuously by microscopy.TheSHK cell line consisted predominantly of fibroblast-like cells.The cell line was able to grow between 20℃and 30℃with the optimum growth at 24℃and with a reduced growth between 12℃and 20℃.The growth rate of the cells increased as the proportion ofFBS increased from 10%to 20%at 28℃with optimum growth at the concentration of 20%.The doubling time of the cells was de-termined to be 44.8 h.Chromosome analysis revealed that 52%of the SHK cells maintained a normal diploid chromosome number(2n=46).The cells were successfully transfected with green fluorescent protein(GFP)reporter plasmids and the expression of GFPgene in the cells indicated the possible utility of the cells in gene expression studies.The cells were infected by lymphosystis diseasevirus(LCDV)and found to be susceptible to the virus in cytopathic effect(CPE)observation.The infection was confirmed by PCRand electron microscopy experiments,which proved the existence of the viral particles in the cytoplasm of the virus-infected cells. | WANG Xianli CHEN Songlin SHA Zhenxia FAN Tingjun WANG Na | 2010 | Journal of Ocean University of China2010，9，2： | 4 |
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