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| 1 | Nucleoporin 88 expression in hepatitis B and C virus-related liver diseases显示文摘AIM: To investigate the expression of nucleoporin 88 (Nup88) in hepatitis B virus (HBV) and C virus (HCV)-related liver diseases. METHODS: We generated a new monoclonal Nup88 antibody to investigate the Nup88 protein expression by immunohistochemistry (IHC) in 294 paraffin-embedded liver specimens comprising all stages of hepatocellular carcinogenesis. In addition, in cell culture experiments HBV-positive (HepG2.2.15 and HB611) and HBV-negative (HepG2) hepatoma cell lines were tested for the Nup88 expression by Western-immunoblotting to test data obtained by IHC.RESULTS: Specific Nup88 expression was found in chronic HCV hepatitis and unspecific chronic hepatitis, whereas no or very weak Nup88 expression was detected in normal liver. The Nup88 expression was markedly reduced or missing in mild chronic HBV infection and inversely correlated with HBcAg expression. Irrespective of the HBV- or HCV-status, increasing Nup88 expression was observed in cirrhosis and dysplastic nodules, and Nup88 was highly expressed in hepatocellular carcinomas. The intensity of Nup88 expression significantly increased during carcinogenesis (P < 0.0001) and correlated with dedifferentiation (P < 0.0001). Interestingly, Nup88 protein expression was significantly downregulated in HBV-positive HepG2.2.15 (P < 0.002) and HB611 (P < 0.001) cell lines as compared to HBV-negative HepG2 cells. CONCLUSION: Based on our immunohistochemical data, HBV and HCV are unlikely to influence the expression of Nup88 in cirrhotic and neoplastic liver tissue, but point to an interaction of HBV with the nuclear pore in chronic hepatitis. The expression of Nup88 in nonneoplastic liver tissue might reflect enhanced metabolic activity of the liver tissue. Our data strongly indicate a dichotomous role for Nup88 in non-neoplastic and neoplastic conditions of the liver. | Martina Knoess Anna Kordelia Kurz Olga Goreva Nuran Bektas Kai Breuhahn Magarethe Odenthal Peter Schirmacher Hans Peter Dienes C Thomas Bock Hanswalter Zentgraf Axel zur Hausen | 2006 | World Journal of Gastroenterology2006，12，36： | 4 |
| 2 | Regulation of Enterocyte Apoptosis by Acyl-CoA Synthetase 5 Splicing显示文摘 | Nikolaus Gassler Wilfried Roth Benjamin Funke Armin Schneider Frank Herzog Jens J.W. Tischendorf Kerstin Grund Roland Penzel Ignacio G. Bravo John Mariadason Volker Ehemann Jaromir Sykora Tobias L. Haas Henning Walczak Tom Ganten Hanswalter Zentgraf Peter | 2007 | Gastroenterology2007，，2： | 2 |
| 3 | Modulation of MAPK pathways and cell cycle by replicating hepatitis B virus: Factors contributing to hepatocarcinogenesis显示文摘 | Ruth Chin Linda Earnest-Silveira Bernd Koeberlein Susanne Franz Hanswalter Zentgraf Xuebin Dong Eric Gowans C.-Thomas Bock Joseph Torresi | 2007 | Journal of Hepatology2007，，3： | 1 |
| 4 | Autophagy-A double- edged sword in ontology显示文摘 | Apel A Hanswalter Z Bti chler MW | 2009 | Int J Cancer2009，125，5： | 1 |
| 5 | Regulation of Enterocyte Apoptosis by Acyl-CoA Synthetase 5 Splicing显示文摘 | Nikolaus Gassler Wilfried Roth Benjamin Funke Armin Schneider Frank Herzog Jens J.W. Tischendorf Kerstin Grund Roland Penzel Ignacio G. Bravo John Mariadason Volker Ehemann Jaromir Sykora Tobias L. Haas Henning Walczak Tom Ganten Hanswalter Zentgraf Peter | 2007 | Gastroenterology2007，，2： | 1 |
| 6 | The human granulocyte nucleus: Unusual nuclear envelope and heterochromatin composition显示文摘 | Ada L. Olins Monika Zwerger Harald Herrmann Hanswalter Zentgraf Amos J. Simon Marc Monestier Donald E. Olins | 2008 | European Journal of Cell Biology2008，，5： | 1 |
| 7 | THE HBx PROTEIN EXPRESSION IN LIVER CANCER显示文摘The expression of HBx protein in liver tissues from 48 cases of different liver diseases, Including 32 cases of hepatocellular carcinoma (HCC), 10 of chronic hepatitis (CH), 2 of angioma and 4 cases of normal liver was studied. These samples were tested for HBx protein, HBsAg by modified ABC method. Positive rates of HBx in cancer and adjacent Uver tissue were 75. 0% and 62. 5%, and positive rates of HBsAg were 37.5% and 78.1% respectively. The occurence of HBx in the absence of HBsAg was more frequently observed in tissues from HCC (46.9%) man CH (0%). The results showed mat expression of HBx was more active than that of HBsAg, and it Is suggested that HBx might be a useful marker for the diagnosis of liver cancer. | 施达仁 何公平 梁小浣 汤钊猷 胡锡琪 Hanswalter Zentgraf | 1993 | Chinese Journal of Cancer Research1993，5，1： | 0 |
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