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| 1 | Differentiation of neuron-like cells from mouse parthenogenetic embryonic stem cells显示文摘Parthenogenetic embryonic stem cells have pluripotent differentiation potentials,akin to fertilized embryo-derived embryonic stem cells.The aim of this study was to compare the neuronal differentiation potential of parthenogenetic and fertilized embryo-derived embryonic stem cells.Before differentiation,karyotype analysis was performed,with normal karyotypes detected in both parthenogenetic and fertilized embryo-derived embryonic stem cells.Sex chromosomes were identified as XX.Immunocytochemistry and quantitative real-time PCR detected high expression of the pluripotent gene,Oct4,at both the mRNA and protein levels,indicating pluripotent differentiation potential of the two embryonic stem cell subtypes.Embryonic stem cells were induced with retinoic acid to form embryoid bodies,and then dispersed into single cells.Single cells were differentiated in N2 differentiation medium for 9 days.Immunocytochemistry showed parthenogenetic and fertilized embryo-derived embryonic stem cells both express the neuronal cell markers nestin,βIII-tubulin and myelin basic protein.Quantitative real-time PCR found expression of neurogenesis related genes(Sox-1,Nestin,GABA,Pax6,Zic5 and Pitx1) in both types of embryonic stem cells,and Oct4 expression was significantly decreased.Nestin and Pax6 expression in parthenogenetic embryonic stem cells was significantly higher than that in fertilized embryo-derived embryonic stem cells.Thus,our experimental findings indicate that parthenogenetic embryonic stem cells have stronger neuronal differentiation potential than fertilized embryo-derived embryonic stem cells. | Xingrong Yan Yanhong Yang Wei Liu Wenxin Geng Huichong Du Jihong Cui Xin Xie Jinlian Hua Shumin Yu Liwen Li Fulin Chen | 2013 | Neural Regeneration Research2013，8，4： | 4 |
| 2 | Distribution and sources of polycyclic aromatic hydrocarbons from urban to rural soils: A case study in Dalian, China显示文摘 | Zhen Wang Jingwen Chen Xianliang Qiao Ping Yang Fulin Tian Liping Huang | 2007 | Chemosphere2007，，5： | 2 |
| 3 | Effects of Diet with 'Lianteng' Additive of Chinese Herbal Medicine on Blood Biochemical Indexes,Immune and Antioxidant Function of Guangxi Sanhuang Chickens显示文摘[Objectives]This trial was conducted to investigate the effects of diet with'Lianteng'additive of Chinese herbal medicine on blood biochemical indexes,immune and antioxidant function of Guangxi Sanhuang chickens.[Methods]480 Guangxi Sanhuang chickens at the age of 1 d were divided into 4 groups randomly and each group was assigned to 6 replicates with 20 chicks in each replicate;groups A,B and C were treatment groups and fed basal diets with 0.1%,0.2%and 0.4%of'Lianteng'additive of Chinese herbal medicine respectively during a trial of 70 d.Group D was control group and fed only basal diet.The serum samples were taken from 6 chicks in each group at the age of 21,35 and 49 d respectively and analyzed for biochemical,immune and antioxidant parameters in serum.[Results]Results from the trial showed that no difference was found in serum content of GLU,TG,TP,TC,and UN in the measurement at the age of 21,35 and 49 d(P>0.05);however,the content of ALB from groups A and B at 35 d was significantly higher than that from group D(P<0.01),and the ALB content from group B at 49 d was also higher than that from group D(P<0.05);the IgM content at 49 d from group A was lower than that from group D(P<0.05),in addition to this,there was no differences found in the contents of IgG,IgM and IFN-y among groups A,B,C and D during the trial(P>0.05);but the content of IL-2 from groups A and B at 49 d was significantly greater than that from group D(P<0.05);T-AOC from groups A,B and C at 49 d was very significantly higher than that from group D(P<0.01),and the one of group B at 35 d was still obviously higher than that of group D(P<0.05);also at 49 d,the GSH content in groups A and B was much greater than that in group D(P<0.01);T-SOD activity of group B and C at 35 d was distinctly greater than that of group D(P<0.05).[Conclusions]It can be concluded that the addition of 0.2%of'Lianteng'additive of Chinese herbal medicine in diet can increase immune function in a certain extent and promote the antioxidant ability observably for Guangxi Sanhuang chickens. | Sicong LUO Wenjie LU Lizhi LU Fulin WANG Qian QIN Cui YANG Jiahuang YANG | 2021 | Medicinal Plant2021，12，1： | 1 |
| 4 | Comparative study between coral-mesenchymal stem cells-rhBMP-2 composite and auto-bone-graft in rabbit critical-sized cranial defect model 显示文摘 | Rui Hou Fulin Chen Yaowu Yang | 2007 | J Biomed Mater Res A2007，80，1： | 1 |
| 5 | 靶向肾癌中的E3连接酶接头蛋白SPOP的小分子抑制剂显示文摘近年来肾癌发病率上升幅度在恶性肿瘤中排名第一。临床治疗表明,肾癌对放疗和化疗均不敏感,以索拉非尼和舒尼替尼为代表的靶向抗肿瘤药物是晚期肾癌的一线治疗药物,但对转移性肾癌的疗效十分有限,并且容易产生耐药。因此,发现并确证治疗肾癌特异性药物作用新靶标是一项十分紧迫并意义重大的任务。 | Zhong-Qiang Guo Tong Zheng Baoen Chen Cheng Luo Sisheng Ouyang Shouzhe Gong Jiafei Li Liu-Liang Mao Fulin Lian Yong Yang Yue Huang Li Li Jing Lu Bidong Zhang Luming Zhou Hong Ding Zhiwei Gao Liqun Zhou Guoqiang Li Ran Zhou Ke Chen Jingqiu Liu Yi Wen Likun Gong Yuwen Ke Shang-Dong Yang Xiao-Bo Qiu Naixia Zhang Jin Ren Dafang Zhong 杨财广 刘江 蒋华良 | 2017 | 科学新闻2017，19，4： | 1 |
| 6 | MDM 2 is a useful prognostic biomarker for resectable gastric cancer显示文摘 | Yang Ye Xuan Li Jingjing Yang Shuhan Miao Shouyu Wang Yansu Chen Xiaowei Xia Xuming Wu Jianbing Zhang Yan Zhou Song He Yongfei Tan Fulin Qiang Gang Li Oluf Dimitri R?e Jianwei Zhou | 2013 | Cancer Sci2013，，5： | 1 |
| 7 | Effects of Diet with'Lianhuang'Additive of Chinese Herb Medicine on Growth Performance and Metabolic Rates of Dietary Nutrients in Donglan Black Chicken显示文摘[Objectives]The study was conducted to evaluate the effects of diet with'Lianhuang'additive of Chinese herbal medicine(LACHM)on growth performance and metabolic rates of dietary nutrients in Donglan black chickens(DBCs).[Methods]80 DBCs aged 1 d were blocked into 4 groups randomly and assigned to 6 replicates in each group with 20 chicks in each replicate.Group A as control group fed basal diet.Groups B,C and D as treatment groups consumed the basal diet added with 0.25%,0.50%and 1.00%of LACHM,respectively during the test period of 70 d.In the end of feeding test,24 DBCs with similar body weight from each group were chosen out and used for a metabolism test to determine the metabolic rates of dietary nutrients from each treatment.The metabolism test was conducted as the same design as the feeding test and the DBCs from each group were fed the same diet as previous test.[Results]The ADG of chickens in the test increased by 5.00%-9.15%(P<0.05)except the stage of 50-70 d,and the individual average body weight(IABW)at 70 d in group B also increased by 4.86%(P<0.05).Compared with group A,the ADG of group C in the stages of 36-49,1-49 and 1-70 d and the IABW in the end of 70 d increased by 5.90%,2.66%,2.68%and 2.57%(P>0.05),respectively.The ADFI in the stage of 36-49 d from group A was 6.04%more than that from group B(P<0.01),and 5.12%and 4.13%more than those from groups C and D(P<0.05),respectively,and the ADFI of group A in the stage of 1-49 d was still 2.86%more than that from group C(P<0.05).However,no differences in ADFI were found in other stages in the test among the 4 groups(P>0.05).The F/G in the stages of 22-35 and 1-70 d from group B was 6.32%and 3.41%lower than those from group A(P<0.05),respectively,and even 11.43%and 7.31%lower in the stages of 36-49 and 1-49 d from group B than those from group A(P<0.01),respectively.The F/G of group C in the stage of 36-49 d was also 10.15%lower than that of group A(P<0.05),and still 5.29%lower than that from group A in 1-49 d(P<0.01),but no differences were found in other stages between the two groups(P>0.05).The metabolic rates of MD,CP,CF,NFE,CA,TE,Ca and P in the 3 treatment diets for group B,C and D all increased to some extent.Among them,the increasing extents of diets with 0.25%and 0.50%of LACHM for groups B and C were much greater.Therefore,the diet with 0.25%of LACHM could improve the growth performance and metabolic rates of dietary nutrients in DBCs significantly.[Conclusions]This study provides a theoretical reference for the effects of traditional Chinese medicine additives on the growth and metabolic rates of DBCs. | Jing YAN Lei QIU Min CHEN Fulin WANG Wenjie LU Menglei LI Cui YANG Jiahuang YANG | 2022 | Agricultural Biotechnology2022，11，3： | 1 |
| 8 | A Review of Chinese Herb Medicine Additives on Chicken Growth Performance显示文摘Researches showed that many Chinese herb medicines can improve chicken growth performance not only by their pharmacological function, but also by the improvement of antioxidant function, immunity and disease resistance of chicken. The menace to human health and environment has occurred since too much antibiotics have been used as growth-promoting additives in long term in chicken industry. Therefore, research on replacement of antibiotics by Chinese herb medicines as additives for chicken growth is underway. This paper summarized some research results in the topic briefly to provide information for researchers and readers. | Cui YANG Yuying LIAO Fulin WANG Jiahuang YANG Zhuyue WU | 2019 | Agricultural Biotechnology2019，8，6： | 1 |
| 9 | A generating method for digital gear tooth surfaces显示文摘 | Fulin Wang Chuanyun Yi Tao Wang Shuzi Yang Gang Zhao | 2006 | The International Journal of Advanced Manufacturing Technology (-)2006，，5： | 1 |
| 10 | Impacts of plants allelopathy compounds on seed germination显示文摘 | Yang Qihe Ye Wanhui Liao Fulin | 2005 | Chinese Journal of Ecology2005，24，12： | 1 |
| 11 | Application of positive matrix factorization to identify potential sources of PAHs in soil of Dalian, China 显示文摘 | Wang Degao Tian Fulin Yang Meng | 2009 | Environmental Pollution2009，157，5： | 1 |
| 12 | A Generating Method for Digital Gear Tooth Surface显示文摘 | Fulin Wang Chuanyun Yi Tao Wang Shuzi Yang | 2006 | The International Journal of Advanced Manufacturing Technology2006，28，： | 1 |
| 13 | Sources and seasonal variation of atmospheric polycyclic aromatic hydrocarbons in Dalian, China: Factor analysis with non-negative constraints combined with local source fingerprints显示文摘 | Fulin Tian Jingwen Chen Xianliang Qiao Zhen Wang Ping Yang Degao Wang Linke Ge | 2009 | Atmospheric Environment2009，，17： | 1 |
| 14 | MoS_(2) nanoflowers coupled with ultrafine Ir nanoparticles for efficient acid overall water splitting reaction显示文摘Bi-functional electrocatalysts for acid overall water splitting reactions are crucial but still challenging to the development of proton exchange membrane water electrolysis.Herein,an efficient bi-functional catalyst of Ir/MoS_(2) nanoflowers(Ir/MoS_(2) NFs) catalyst was reported for acidic water electrolysis which can be constructed by coupling three-dimensionally interconnected MoS_(2) NFs with ultrafine Ir nanoparticles.A more suitable adsorption ability for the H* and *OOH intermediates was revealed,where the Ir sites were proposed as the main active center and MoS_(2) promoted the charge relocation to electronically modify the interfacial structure.The significant interfacial charge redistribution between the MoS_(2) NFs and the Ir active sites synergistically induced excellent catalytic activity and stability for the water electrolysis reaction.Specifically,the catalyst required overpotentials of 270 and 35 mV to reach a kinetic current density of 10 mA cm^(-2)for OER and HER,respectively,loading on the glass carbon electrode,with high catalytic kinetics,stability,and catalytic efficiency.A two-electrode system constructed by Ir/MoS_(2) NFs drove 10 mA cm^(-2)at a cell voltage of 1.55 V,about 70 mV lower than that of the commercial Pt/C||IrO_(2) system.In addition,partial surface oxidation of Ir nanoparticles to generate high-valent Ir species was also found significant to accelerate OER.The enhanced catalytic performance was attributed to the strong metal-support interaction in the Ir/MoS_(2) NFs catalyst system that changed the electronic structure of Ir metal and promoted the synergistic catalytic effect between Ir and MoS_(2) NFs.The work presented a novel platform of Ir-catalyst for proton exchange membrane water electrolysis. | Chunyan Wang Lice Yu Fulin Yang Ligang Feng | 2023 | Journal of Energy Chemistry2023，，12： | 0 |
| 15 | Interannual Changes of Land Surface Radiation Components in Loess Plateau and Their Responses to Climate Change显示文摘In this paper,land surface observation data at semi-arid climate and environmental observation station( SACOL station) of Lanzhou University during 2006- 2012 and the data of Yuzhong meteorological station were used to analyze the responses of land surface radiation budget components to climate fluctuation,study the interannual variability of surface albedo,and discuss the feedback of various land surface process parameters on the interannual fluctuations of temperature and precipitation in Loess Plateau. According to the type of precipitation in Loess Plateau,the year was divided into winter and summer in order to get more significant interannual variability and correlation. The results showed that the trends of temperature and precipitation during 2006- 2012 were consistent with the warming and drying total trend in recent years in Loess Plateau. Shallow surface soil moisture and temperature showed a good response to temperature and precipitation,and the annual variation of summer half year had greater impact on the trend in the whole year. Incident solar radiation increase was major reason for climate warming in the Loess Plateau region.The combined effect of climatic factors was the reason for the change of surface albedo. Through the distinguish inquiry by winter and summer data,it was obtained that most correlations between summer radiation components and climatic factors have been improved,and partial correlations between winter radiation components and climatic factors have been increased. | Xia Lu Zhang Qiang Yue Ping Yang Fulin | 2014 | Meteorological and Environmental Research2014，5，11： | 0 |
| 16 | Effects of Chinese Herbal Medicine Feed Additives on Growth Performance and Dietary Nutrient Metabolism of Black-bone Chickens显示文摘[Objectives]To study the effects of Chinese herbal medicine feed additives on the growth performance and dietary nutrient metabolic rate of black-one chickens.[Methods]480 pieces of 1-day-old Donglan Black-bone Chickens were selected and divided into 4 groups,with 6 replicates in each group,with 20 pieces in each replicate.Among them,group A was the control group and was fed the basal diet;the groups B,C,and D were the experimental groups,which were separately supplemented with 0.25%,0.50%and 1.00%of Chinese herbal medicine additives in the basal diet,and the experimental period was 70 d.At the end of the feeding experiment,24 experimental chickens with similar body weight were selected from each group,and the same design of the feeding experiment was used to continue to feed the original diet for the metabolic experiment to determine the nutrient metabolic rate.[Results]Except the 50-70 d stage,the average daily gain(ADG)of group B in other stages was increased by 5.00%-9.15%(P<0.05),and the average body weight of group B at 70 d was also increased by 4.86%(P<0.05);the ADG of group C at 36-49,1-49 and 1-70 d and the average individual weight at 70 d were also higher than that of group A by 5.90%,2.66%,2.68%and 2.57%,respectively(P>0.05);at 36-49 d,the average daily feed intake(ADFI)of group A was higher than that of group B by 6.04%(P<0.01),and was also higher than that of groups C and D by 5.12%and 4.13%(P<0.05),respectively;at 1-49 d,the ADFI of group A was higher than that of group C by 2.86%(P<0.05),and there was no significant difference between the four groups of experimental chickens at each stage(P>0.05);at 22-35 and 1-70 d,the feed to gain ratio(F/G)of group B was 6.32%and 3.41%lower than that of group A(P<0.05),and at 36-49 and 1-49 d,the F/G of group B was even lower;at 36-49 d,the F/G of group C was 10.15%lower than that of group A(P<0.05),and at 1-49 d,it was also lower than that of group A by 5.29%(P<0.01);there was no significant difference between the two groups at other stages.In terms of nutrient metabolism:the three different amounts of this additive have increased the metabolic rate of DM,CP,CF,NFE,CA,Ca,P,TE and other nutrients in the diet to varying degrees,especially the addition of 0.25%and 0.50%had the significant increase.[Conclusions]The addition of 0.25%Chinese herbal medicine additives in the diet can significantly improve the growth performance and main nutrient metabolic rate of Donglan Black-bone Chickens. | Cui YANG Fulin WANG Siqian WU Zhuyue WU Limei QIN Huofu WAN Zhengzhong XIAO Chunhua HUANG Jiahuang YANG Fengying WEI | 2022 | Asian Agricultural Research2022，14，11： | 0 |
| 17 | Synergistic Effect of Alkali Metals in Coal and Introduced CaO during Steam Gasification显示文摘The steam gasification kinetics of Zhundong raw coal and the washed coal by deionized water or hydrochloric acid with/without addition of CaO were tested by thermogravimetric analyzer(TGA)at medium temperatures(650℃ to 800℃).The cation contents of potassium and sodium in samples were determined by Inductively Coupled Plasma Optical Emission Spectrometer(ICP-OES).The Brunauer-Emmett-Teller(BET)surface area of the samples was tested by N2 adsorption,and the morphologies of the samples were characterized by scanning electron microscopy(SEM).Experimental results showed that the organic sodium was the main catalyst for the gasification of the pyrolysis char,and the gasification rate of the char could be enhanced further with the introduction of CaO.The inherent alkali metals in coal and the introduced CaO showed a synergistic effect that occurred obviously above 735℃.The char conversion curves with or without CaO were fitted by the modified volumetric model(MVM).The calculated results indicated that the addition of CaO increased the pre-exponential factor obviously,but made little changes on the activation energy.It was proposed that the synergistic effect was resulted from the co-melting of the sodium and CaO,which facilitated the migration of the catalyst ions and the generation of C(O)intermediates for the gasification. | LIU Yang YANG Xinfang LEI Fulin XIAO Yunhan | 2020 | Journal of Thermal Science2020，29，6： | 0 |
| 18 | UBA3 promotes the occurrence and metastasis of intrahepatic cholangiocarcinoma through MAPK signaling pathway显示文摘Intrahepatic cholangiocarcinoma(icC)accounts for approximately 15%of primary liver cancers,and the incidence rate has been increasing in recentyears.Surgical resection is the best treatment for IcC,but the 5-year survival rate is less than 30%.IcC signature genes are crucial for the early diagnosis of IcC,so it is especially important to identify signature genes.The aim of this study is to screen the signature genes of IcC and find the potential target for the treatment of ICC.We find that UBA3 is highly expressed in ICC,and knockdown of UBA3 inhibits ICC proliferation,invasion and migration.Mechanistic experiments show that UBA3 promotes IcC proliferation,invasion and mi-gration by affecting ANXA2 through the MAPK signaling pathway.UBA3 is a target of bufalin,and bufalin targeting UBA3 inhibits ICC development and progression through the MAPK signaling pathway.In conclusion,our study shows that bufalin inhibits IcC by targeting UBA3,which has emerged as a new biomarker and potential therapeutic target forIcc. | Huhu Zhang Jiahua Yang Qinghang Song Xiaoyan Ding Fulin Sun Lina Yang | 2024 | Acta Biochimica et Biophysica Sinica2024，56，2： | 0 |
| 19 | Structural and functional studies of erythrocyte membrane-skeleton by single-cell and single-molecule techniques显示文摘As the indispensable oxygen-transporting cells,erythrocytes exhibit extreme deformability and amazing stability as they are subject to huge reversible shear stress and extrusion force during massive circulation in the body.The unique architecture of spectrin-actin-based membraneskeleton is considered to be responsible for such excellent mechanical properties of erythrocytes.Although erythrocytes have been recognized for more than 300 years,myriad questions about membrane-skeleton constantly attract people's attention.Here,we summarize the kinds of distinctive single-cell and single-molecule techniques that were used to investigate the structure and function of erythrocyte membrane-skeleton at macro and micro levels. | Fulin Xing Fen Hu Jianyu Yang Leiting Pan Jingjun Xu | 2019 | Journal of Innovative Optical Health Sciences2019，，1： | 0 |
| 20 | Effects of Different Protein Energy Ratios on Fattening Performance,Slaughter Performance and Meat Quality of Pigs显示文摘[Objectives]Protein energy ratio refers to the proportional relationship between protein and energy levels in animal diets,i.e.,the grams of crude protein corresponding to every megacalorie of energy,which is generally expressed as CP:ME or CP:DE.This study was conducted to investigate the effects of different diets on fattening and slaughter performance and meat quality traits for'L(Large Yorkshire)×L(Landrace)'crossbred pigs.[Methods]Eighteen piglets of L×L crossbred with similar body weights about 51 kg were selected.The piglets were divided into 3 groups randomly and each group was assigned to 3 replicates with 2 piglets in a replicate.Group A was fed diet Ⅰ (control diet),group B was fed the same diet of group A in the first month of the trial but fed diet Ⅱ in second month,and group C consumed diet Ⅲ.All the pigs were fed in the same feeding condition for two months except the different diets during the trial.One pig in similar body weight from each replicate was slaughtered for the determination of slaughter performance and meat quality traits in the end of the trial.[Results]The average daily feed intake(ADFI)of pigs from group B was only(2.32±0.52)kg and significantly lower than those from group A and group C(P<0.05),but no difference was found in average daily gain(ADG) and feed/gain(P>0.05).Also,no differences occurred in the carcass length,back fat thickness,longissimus muscle (LM) area and dressing percentage(P>0.05).In addition,no differences were found in the meat quality traits of shear force,meat color,pH_(45 min),pH_(24 h) and cooking loss(P>0.05).However,the water-holding capacity of meat from group C was(2.58±0.02)ms and significantly lower than that of(2.80±0.20)ms from group A(P<0.05).Although the contents of glutamic acid and cystine in LM from group B was significantly lower than those from group A and group C(P<0.05),no differences occurred in the contents of other amino acids,the total amino acid and total flavor amino acid among the three groups(P>0.05).However,the inosine monophosphate content of LM from group C was only(331.80±11.53)mg/100 g and significantly lower than those of(361.00±6.36)and(366.37±4.80)mg/100 g from group A and B(P<0.05).Even though no differences were found in the contents of DM and CP in LM among the three groups of pigs,the content of intramuscular fat(IMF)in LM from group B and group C was increased by 45.6%and 46.58%respectively from that of group A(P<0.05),but no difference occurred between group B and C(P>0.05).[Conclusions]DietsⅡ and Ⅲ in this study caused no differences in fattening and slaughter performance of L×L crossbred pigs,but the effects on some meat traits were still significant,especially on the improvement of intramuscular fat in experimental pigs.Therefore,they could improve the meat quality of crossbred pigs to a certain extent. | Cui YANG Rongsheng JIANG Yongshao WU Xiying YANG Haihong LIAO Qian QIN Shishan QIN Ying ZHANG Jiahuang YANG Limei QIN Fulin WANG | 2023 | Agricultural Biotechnology2023，12，2： | 0 |
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