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| 1 | CD74 is a survival receptor on colon epithelial cells显示文摘AIM: To investigate the expression and function of CD74 in normal murine colon epithelial cells (CEC) and colon carcinoma cells. METHODS: Expression of CD74 mRNA and protein were measured by reverse transcriptase-polymerase chain reaction (RT-PCR), Western blotting and fluorescence-activated cell sorter (FACS). The effect of migration inhibitory factor (MIF) on the survival of normal CEC from C57BL/6, NOD/SCID, and CD74 def icient mice both in vitro and in vivo, and on the CT26 carcinoma cell line was analyzed by (quantitative) qRT-PCR, RTPCR, Western blotting and FACS. RESULTS: CD74 was found to be expressed on normalCEC. Stimulation of CD74 by MIF induced a signaling cascade leading to up-regulation of Bcl-2 expression, resulting in a signif icant increased survival of CEC. CD74 was also expressed on the CT26 colon carcinoma cell line and its stimulation by MIF resulted in enhanced cell survival, up-regulation of Akt phosphorylation and Bcl-2 expression. | Nitsan Maharshak Sivan Cohen Frida Lantner Gili Hart Richard Bucala Idit Shachar | 2010 | World Journal of Gastroenterology2010，16，26： | 9 |
| 2 | Improved cryopreservation of human hepatocytes using a new xeno free cryoprotectant solution显示文摘AIM:To optimize a xeno-free cryopreservation protocol for primary human hepatocytes.METHODS:The demand for cryopreserved hepatocytes is increasing for both clinical and research purposes.Despite several hepatocyte cryopreservation protocols being available,improvements are urgently needed.We first compared controlled rate freezing to polystyrene box freezing and did not find any significant change between the groups.Using the polystyrene box freezing,we compared two xeno-free freezing solutions for freezing of primary human hepatocytes:a new medium(STEM-CELLBANKER,CB),which contains dimethylsulphoxide(DMSO) and anhydrous dextrose,both permeating and non-permeating cryoprotectants,and the frequently used DMSO-University of Wisconsin(DMSOUW) medium.The viability of the hepatocytes was assessed by the trypan blue exclusion method as well as a calcein-esterase based live-dead assay before and after cryopreservation.The function of the hepatocytes was evaluated before and after cryopreservation by assessing enzymatic activity of 6 major cytochrome P450 isoforms(CYPs):CYP1A2,CYP2C9,CYP2C19,CYP2D6,CYP3A4 and CYP3A7.RESULTS:The new cryoprotectant combination preserved hepatocyte viability significantly better than the standard DMSO-UW protocol(P < 0.01).There was no significant difference in viability estimation between both the trypan blue(TB) and the Live-Dead Assay methods.There was a correlation between viability of fresh hepatocytes and the difference in cell viability between CB and DMSO protocols(r 2 = 0.69) using the TB method.However,due to high within-group variability in the activities of the major CYPs,any statistical between-group differences were precluded.Cryopreservation of human hepatocytes using the cryoprotectant combination was a simple and xeno-free procedure yielding better hepatocyte viability.Thus,it may be a better alternative to the standard DMSO-UW protocol.Estimating CYP activities did not seem to be a relevant way to compare hepatocyte function between different groups due to high normal variability between different liver samples.CONCLUSION:The cryoprotectant combination may be a better alternative to the standard DMSO-UW protocol in primary human hepatocyte cryopreservation. | Mohammed Saliem Frida Holm Rosita Bergstrm Tengzelius Carl Jorns Lisa-Mari Nilsson Bo-Gran Ericzon Ewa Ellis Outi Hovatta | 2012 | World Journal of Hepatology2012，4，5： | 2 |
| 3 | TAp63regulates VLA-4 expression and chronic lymphocytic leukemia cell migration to the bone marrow in a CD74-dependent manner显示文摘 | INBAL BINSKY FRIDA LANTNER LEV SHVIDEL | 2010 | J Immunol2010，184，9： | 1 |
| 4 | Breeding research on rose pigments. II. Combining ability analyses of variance of four flavonoids in F1 populations显示文摘 | D. P. Vries Frida Garretsen Lidwien A. M. Dubois H. A. Keulen | 1980 | Euphytica1980，，1： | 1 |
| 5 | Synthesis of new photochromic polymers based on phe-noxynaphthacenequinone显示文摘 | Frida Buchholtz Alexander Zelichenok Valeri Krongauz | 1993 | Macromolecules1993，26，5： | 1 |
| 6 | Matrix Protein 2 of Influenza A Virus Blocks Autophagosome Fusion with Lysosomes显示文摘 | Monique Gannagé Dorothee Dormann Randy Albrecht J?rn Dengjel Tania Torossi Patrick C. R?mer Monica Lee Till Strowig Frida Arrey Gina Conenello Marc Pypaert Jens Andersen Adolfo García-Sastre Christian Münz | 2009 | Cell Host & Microbe2009，，4： | 1 |
| 7 | Matrix Protein 2 of Influenza A Virus Blocks Autophagosome Fusion with Lysosomes显示文摘 | Monique Gannagé Dorothee Dormann Randy Albrecht J?rn Dengjel Tania Torossi Patrick C. R?mer Monica Lee Till Strowig Frida Arrey Gina Conenello Marc Pypaert Jens Andersen Adolfo García-Sastre Christian Münz | 2009 | Cell Host & Microbe2009，，4： | 1 |
| 8 | Teaching Business Ethics:Questioning the Assumptions,Seeking New Directions显示文摘 | FURMAN FRIDA KERNER | 1990 | Journal of Business Ethics1990，9，1： | 1 |
| 9 | Epicardial Adipose Tissue and Insulin Resistance in Obese Subjects显示文摘 | Gianluca Iacohellis Frida Leonetti | 2005 | J Clin Endocrinol Metab2005，90，： | 1 |
| 10 | Distribution of bacterioplankton in meromictic lake saelenvannet,as setermined by denaturing gradient gel electrophoresis of PCR-amplified gene fragments coding for 16S rRNA显示文摘 | Lise V Larry F Frida L D | 1997 | Appl Environ Microbiol1997，63，9： | 1 |
| 11 | Com- par, ative genomics of Lbx loci reveals conservation of identical Lbx ohnologs in bony vertebrates显示文摘 | Karl R Wotton Frida K Weierud Katharine E Lewis | 2008 | BMC Evolutionary Biol- ogy2008，8，： | 1 |
| 12 | Impact of Laparoscopic Sleeve Gastrectomy on Upper Gastrointestinal Symptoms显示文摘 | Marilia Carabotti Gianfranco Silecchia Francesco Greco Frida Leonetti Luca Piretta Marco Rengo Mario Rizzello John Osborn Enrico Corazziari Carola Severi | 2013 | Obesity Surgery2013，，10： | 1 |
| 13 | Tax Structure and Growth:Are Some Taxes Better Than Others?显示文摘 | Frida Widmalm | 2001 | Public Choice2001，，3： | 1 |
| 14 | Statistical analysis of growthcurves in plant breeding显示文摘 | Keuls M and Frida Garretsen | 1982 | Euphytica1982，31，1： | 1 |
| 15 | Genetic association analysis of the interleukin 7 gene(IL7)in multiple sclerosis显示文摘 | Frida L Kristina D An H | 2007 | J Neuroimmunol2007，192，12： | 1 |
| 16 | Topology Optimization of AStamping Die显示文摘 | Anna Nilsson Frida Birath | 2007 | Materials Processing and Design2007，，： | 1 |
| 17 | Analysis of genes coding for CD46,CD55,and C4b-binding protein in patients with idiopathic,recurrent,spontaneous pregnancy loss显示文摘 | Frida C M Eric M Veronique F B | 2013 | Eur J Immunol2013，43，： | 1 |
| 18 | Synthesis of new photochromic polymers based on phenoxynaphthacenequinone显示文摘 | Zelichenok Alexander Krongauz Valeri | 1993 | Macromolecules1993，26，5： | 1 |
| 19 | The Softening Bureaucracy:Accommodating New ResearchOpportunities in the Entrepreneurial University显示文摘 | Alexander Styhre Frida Lind | 2010 | Scandinavian Journal of Management2010，26，2： | 1 |
| 20 | Efficiency of Printed Materials in Worksite Health Promotion显示文摘 | Natalie Kishchuk Frida Anbar Jennifer O' Loughlin | 1991 | American Jour- nal of Health Promotion1991，5，5： | 1 |
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