|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Loss of clusterin both in serum and tissue correlates with the tumorigenesis of esophageal squamous cell carcinoma via proteomics approaches显示文摘AIM: To identify the differentially secreted proteins or polypeptides associated with tumorigenesis of esophageal squamous cell carcinoma (ESCC) from serum and to find potential tumor secreted biomarkers.METHODS: Proteins from human ESCC tissue and its matched adjacent normal tissue; pre-surgery and postsurgery serum; and pre-surgery and normal control serum were separated by two-dimensional electrophoresis (2-DE)to identify differentially expressed proteins. The silverstained 2-DE were scanned with digital ImageScanner and analyzed with ImageMaster 2D Elite 3.10 software. A cluster of protein spots differentially expressed were selected and identified with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). One of the differentially expressed proteins, clusterin, was downregulated in cancer tissue and pre-surgery serum, but it was reversed in post-surgery serum. The results were confirmed by semi-quantitative reverse-transcription (RT)-PCR and western blot.RESULTS: Comparisons of the protein spots identified on the 2-DE maps from human matched sera showed that some proteins were differentially expressed, with most of them showing no differences in composition, shape or density.Being analyzed by MALDI-TOF-MS and database searching,clusterin was differentially expressed and down-regulated in both cancer tissue and pre-surgery serum compared with their counterparts. The results were also validated by RTPCR and western blot.CONCLUSION: The differentially expressed clusterin may play a key role during tumorigenesis of ESCC. The 2DE-MS based proteomic approach is one of the powerful tools for discovery of secreted markers from peripheral. | Li-YongZhang Wan-TaoYing You-ShengMao Hong-ZhiHe YuLiu Hui-XinWang FangLiu KunWang De-ChaoZhang YingWang MinWu Xiao-HongQian Xiao-HangZhao | 2003 | World Journal of Gastroenterology2003，9，4： | 26 |
| 2 | Expression of MRP14 gene is frequently down-regulated in Chinese human esophageal cancer显示文摘Migration inhibitory factor-related protein 14 (MRP 14) is one of calcium-binding proteins,referred as S 100A9. The heterodimeric molecule formed by MRP 14 with its partner MRP8 (S 100A8) is the major fatty acid carrier in neutrophils.The MRP8/14 complex has been also implicated in the intracellular transport of arachidonic acid and its precursors in keratinocytes. We show here the involvement of MRP14 in human esophageal cancer. In an initial study,mRNA differential display - reverse transcription polymerase chain reaction (DD-PCR) was performed with two esophageal carcinomas,one esophageal adenocarcinoma and matched normal adjacent mucosa. DD-PCR with the arbitrary primer OPA3 showed that one cDNA band was highly expressed in normal tissues,but disappeared or substantially decreased in tumor counterparts. It was later identified to be the 3'-end of migration inhibitory factor-related protein 14 (MRP14).Northern blotting,RT-PCR and Western blotting corroborated the down-regulation of MRP14 in 58/64 squamous cell carcinomas and 2/2 adenocarcinomas as compared with adjacent normal epithelia of the esophagus. MRP14 was undetectable in 3/3 esophageal-carcinoma cell lines. Immunochemistry demonstrated that expression of MRP14 was restricted to normal esophageal epithelia. No mutation was found in the genomic DNA of the MRP14 gene by PCR and directed DNA sequencing. Our finding suggested that the reduction of MRP14 expression is a frequent event in Chinese human esophageal cancer. | JieWANG YanCAI HaoXU JunZHAO XinXU YaLingHAN ZhiXiongXU BaoShengCHEN HaiHU MinWU MingRongWANG | 2004 | Cell Research2004，14，1： | 17 |
| 3 | Enhancement of germ cell apoptosis induced by ethanol in transgenic mice overexpressing Fas Ligand显示文摘It was suggested that chronic ethanol exposure could result in testicular germ cell apoptosis, but the mechanism is still unclear. In the present study, we use a model of transgenic mice ubiquitously overexpressing human FasL to investigate whether Fas ligand plays a role in ethanol-induced testicular germ cell apoptosis. Both wild-type (WT)mice and transgenic (TG) mice were treated with acute ethanol (20% v/v) by introperitoneal injection for five times.After ethanol injection, WT mice displayed up-regulation of Fas ligand in the testes, which was shown by FITCconjugated flow cytometry and western blotting. Moreover, TG mice exhibited significantly more apoptotic germ cells than WT mice did after ethanol injection, which was demonstrated by DNA fragmentation, PI staining flow cytometry and TUNEL staining. In addition, histopathological examination revealed that degenerative changes of epithelial component of the tubules occurred in FasL overexpressing transgenic mice while testicular morphology was normal in wild-type mice after acute ethanol exposure, suggesting FasL expression determines the sensitivity of testes to ethanol in mice. In summary, we provide the direct evidences that Fas ligand mediates the apoptosis of testicular germ cells induced by acute ethanol using FasL transgenic mice. | JIAHUAHU JIEJIANG YINGHUAMA NAYANG MAOHUZHANG MINWU JIANFEI LIHEGUO | 2003 | Cell Research2003，13，5： | 16 |
| 4 | Loss of myeloid-related proteins 8 and myeloid-related proteins 14 expression in human esophageal squamous cell carcinoma correlates with poor differentiation显示文摘To study the expression of myeloid-related proteins(MRP)8 and myeloid-related proteins(MRP)14 in humanesophageal squamous cell carcinoma and to investigate ifthere was any correlation between MRP8 and MRPI4expression level and histopathological grade in thesetumors. | Jian-PingKong FangDing Chuan-NongZhou Xiu-Qinwang Xiao-PingMiao MinWu Zhi-HuaLiu | 2004 | World Journal of Gastroenterology2004，10，8： | 13 |
| 5 | Gene expression profiles at different stages of human esophageal squamous cell carcinoma显示文摘AIM: To characterize the gene expression profiles in differentstages of carcinogenesis of esophageal epithelium.METHODS: A microarray containing 588 cancer relatedgenes was employed to study the gene expression profileat different stages of esophageal squamous cell carcinomaincluding basal cell hyperplasia, high-grade dysplasia,carcinoma in situ, early and late cancer. Principle componentanalysis was performed to search the genes which wereimportant in carcinogenesis.RESULTS: More than 100 genes were up or down regulatedin esophageal epithelial cells during the stages of basal cellhyperplasia, high-grade dysplasia, carcinoma in situ, earlyand late cancer. Principle component analysis identified aset of genes which may play important roles in the tumordevelopment. Comparison of expression profiles betweenthese stages showed that some genes, such as P160ROCK,JNK2, were activated and may play an important role inearly stages of carcinogenesis. CONCLUSION: These findings provided an esophagealcancer-specific and stage-specific expression profiles,showing that complex alterations of gene expression underliethe development of malignant phenotype of esophagealcancer cells. | JinZhou Li-QunZhao Mo-MiaoXiong Xiu-Qinwang Guan-RuiYang Zong-LiangQiu MinWu Zhi-HuaLiu | 2003 | World Journal of Gastroenterology2003，9，1： | 11 |
| 6 | Translocation of annexin I from cellular membrane to the nuclear membrane in human esophageal squamous cell carcinoma显示文摘AIM: To investigate the alteration of the annexin I subcellular localization in esophageal squarnous cell carcinoma (ESCC)and the correlation between the translocation and the tumorigenesis of ESCC.METHODS: The protein localization of annexin I was detected in both human ESCC tissues and cell line via the indirect immunofluorescence strategy.RESULTS: In the normal esophageal epithelia the annex in I was mainly located on the plasma membrane and formed a consecutive typical trammels net. Annexin I protein also expressed dispersively in cytoplasm and the nuclei without specific localization on the nuclear membrane. In esophageal cancer annexin I decreased very sharply with scattered disappearance on the cellular membrane, however it translocated and highly expressed on the nuclear membrane,which was never found in normal esophageal epithelia. In cultured esophageal cancer cell line annexin I protein was also focused on the nuclear membrane, which was consistent with the result from esophageal cancer tissues.CONCLUSION: This observation suggests that the translocation of annexin I protein in ESCC may correlate with the tumorigenesis of the esophageal cancer. | YuLiu Hui-XinWang NingLu You-ShengMao FangLiu YingWang Hai-RongZhang KunWang MinWu Xiao-HangZhao | 2003 | World Journal of Gastroenterology2003，9，4： | 10 |
| 7 | Downregulation of retinoic acid receptor-β_z expression is linked to aberrant methylation in esophageal squamous cell carcinoma cell lines显示文摘AIM:To study the role of hypermethylation in the loss of retinoic acid receptor β2(RARβ2) in esophageal squamous cell carcinoma (ESCC).METHODS:The role of hypermethylation in RARβ2 gene silencing in 6 ESCC cell lines was determined by methylation-specific PCR (MSP),and its methylation status was compared with RARβ2 mRNA expression by RT-PCR.The MSP results were confirmed by bisulfite sequencing of RARβ2promoter regions.RESULTS:Methylation was detected in 4 of the 6 cell lines,and the expression of RARβ2 was markedly downregulated in 3 of the 4 methylated cell lines. The expression of RARβ2 was restored in one RARβ2-downregulated cell line with the partial demethylation of promoter region of RARβ2 after 5-aza-2′-deoxycytidine (5-aza-dc) treatment.CONCLUSION:The methylation of the 5′ region may play an important role in the downregulation of RARβ2 in some ESCC cell lines, suggesting that multiple mechanisms contribute to the loss of RARβ2expression in ESCC cell lines.This study may have clinical applications for treatment and prevention of ESCC. | Zhong-MinLiu FangDing Ming-ZhouGuo Li-YongZhang MinWu Zhi-HuaLiu | 2004 | World Journal of Gastroenterology2004，10，6： | 10 |
| 8 | Gene expression profiles of hepatoma cell line HLE显示文摘AIM: To investigate the global gene expression of cancer related genes in hepatoma cell line HLE using Atlas Human Cancer Array membranes with 588 well-characterized human genes related with cancer and tumor biology.METHODS: Hybridization of cDNA blotting membrane was performed with 32P-labeled cDNA probes synthesized from RNA isolated from Human hepatoma cell line HLE and noncirrhotic normal liver which was liver transplantation donor.AtlasImage, a software specific to array, was used to analyze the result. The expression pattern of some genes identified by Atlas arrays hybridization was confirmed by reverse transcription polymerase chain reaction (RT-PCR)in 24 pairs of specimens and Northern blot of 4 pairs of specimens.RESULTS: The differential expression of cell cycle/growth regulator in hepatocellular carcinoma (HCC) showed a stronger tendency toward cell proliferation with more than 1.5-fold up-regulation of Cyclin C, ERK5, ERK6, E2F-3, TFDP2 and CK4. The anti-apoptotic factors such as Akt-1 were up-regulated, whereas the promotive genes of apoptosis such as ABL2 were down-regulated. Among oncogene/tumors suppressors, SKY was down-regulated. Some genes such as Integrin beta 8, Integrin beta 7, DNA-PK, CSPCP,byglycan, Tenacin and DNA Topo were up-regulated. A number of genes, including LAR, MEK1, eps15, TDGF1,ARHGDIA were down-regulated. In general, expression of the cancer progression genes was up-regulated, while expression of anti-cancer progression genes was downregulated. These differentially expressed genes tested with RT-PCR were in consistent with cDNA array findings.CONCLUSION: Investigation of these genes in HCC is helpful in disclosing molecular mechanism of pathogenesis and progression of HCC. For the first time few genes were discovered in HCC. Further study is required for the precise relationship between the altered genes and their correlation with the pathogenesis of HCC. | Lian-XinLiu Zhi-HuaLiu Hong-ChiJiang Wei-HuiZhang Shu-YiQi JieHu Xiu-QinWang MinWu | 2003 | World Journal of Gastroenterology2003，9，4： | 10 |
| 9 | Alterations in expression,proteolysis and intracellular localizations of clusterin in esophageal squamous cell carcinoma显示文摘AIM: To investigate biogenesis and intracellular Iocalizations of clusterin to elucidate the potential molecular mechanisms implicated in tumorigenesis of esophageal mucosa.METHODS: Semi-quantitative RT-PCR for multi-region alteration analysis, Western blot for different transcriptional forms and immunohistochemical staining for intracellular Iocalizations of clusterin were carried out in both tissues and cell lines of ESCC.RESULTS: The N-terminal deletions of the clusterin gene and the appearance of a 50-53 ku nuclear clusterin, an uncleaved, nonglycosylated, and disulfide-linked isoform,were the major alterations in cancer cells of esophagus.Naturally the 40 ku clusterin was located in the connective tissue of the lamina propria of epithelial mucosa and right under the basal membrane of epithelia, but it was disappeared in stromal mucosa of esophagus and the pre-matured clusterin was found positive in cancerous epithelia.CONCLUSION: The N-terminal deletion of clusterin may be essential for its alterations of biogenesis in ESCC. | Hong-ZhiHe Zhen-MeiSong KunWang Liang-HongTeng FangLiu You-ShengMao NingLu Shang-ZhongZhang MinWu Xiao-HangZhao | 2004 | World Journal of Gastroenterology2004，10，10： | 7 |
| 10 | Delay-dependent criteria for the robust stability of systems with time-varying delay显示文摘The problem of delay-dependent robust stability for systems with titne-varying delay has been considered. By using the S-procedure and the Park' s inequality in the recent issue, a delay-dependent robust stability criterion which is less conservative than the previous results has been derived for time-delay systems with time-varying structured uncertainties. The same idea has also been easily extended to the systems with nonlinear perturbations. Numerical examples illustrated the effectiveness and the improvement of the proposed approach. | MinWU YongHE JinhuaSHE | 2003 | 控制理论与应用(英文版)2003，1，1： | 2 |
| 11 | Overexpression of ETS2 in human esophageal squamous cell carcinoma显示文摘AIM: To study the expression pattern of ETS2 (erythroblastosisvirus oncogene homolog 2) in human esophageal squamouscell carcinoma (ESCC).METHODS: Reverse transcription polymerase chain reaction(RT-PCR) and Northern blot were performed to examinethe expression level of ETS2 mRNA in 37 pairs of ESCCtissue samples. Western blot and immunohistochemistrywere carried out to check the expression level of ETS2 proteinin 30 pairs of ESCC tissue specimens.RESULTS: RT-PCR and Northern blot analysis showed thatETS2 mRNA upregulated in 75.7 % (28/37) examined ESCCtissues relative to matched normal tissues. From those 37cases, 14 cases were randomly selected to perform Westernblot and the results revealed that ETS2 protein overexpressedin 71.4 % (10/14) checked ESCC tissues compared with thecorresponding normal tissues. Moreover, the expressionpatterns of ETS2 protein in those 14 cases were identical tothose of ETS2 mRNA displayed by RT-PCR or Northern Blot.Immunohistochemistry analysis showed that the expressionlevel of ETS2 protein rose in 75 % (12/16) tumor epithelialcells contrasted to the normal cells. Altogether the expressionlevel of ETS2 protein increased in 73.3 % (22/30) checkedESCC tissue samples contrary to their normal counterparts.CONCLUSION: The results suggested that ETS2overexpressed in paired human ESCC tissue samples at bothmRNA and protein levels and may be associated with thetumorigenesis of esophagus. | XinLi Jia-YunLu Li-QunZhao Xiu-QinWang Gui-LinLiu ZhongLiu Chuan-NongZhou MinWu Zhi-HuaLiu | 2003 | World Journal of Gastroenterology2003，9，2： | 2 |
| 12 | Expression patterns of esophageal cancer deregulated genes in C57BL/6J mouse embryogenesis显示文摘To investigate the expression patterns of esophagealsquamous cell cancer deregulated genes in mid to late stagesof C57BL/6J mouse embryogenesis, and the correlationbetween these genes in embryonic development andtumorigenesis of esophageal squamous cell cancer. | JianZhang Fu-LuGao Hui-YingZhi Ai-PingLuo FangDing MinWu Zhi-HuaLiu | 2004 | World Journal of Gastroenterology2004，10，8： | 2 |
| 13 | New delay-dependent stability criteria for T-S fuzzy systems with time-varying delay显示文摘 | Fang Liu MinWu Yong He Ryuichi Yokoyama | 2008 | Fuzzy Sets and Systems2008，，： | 1 |
| 14 | Higher Level Orderings on Modules显示文摘The aim of this paper is to investigate higher level orderings on modules over commutative rings. On the basis of the theory of higher level orderings on fields and commutative rings, some results involving existence of higher level orderings are generalized to the category of modules over commutative rings. Moreover, a strict intersection theorem for higher level orderings on modules is established. | MinWU GuangXingZENG | 2005 | Acta Mathematica Sinica,English Series2005，21，2： | 1 |
| 15 | 显示文摘 | Sadia Ameen Ansari S G Minwu Song | 2009 | Superlat- rices and Microstructures2009，46，： | 1 |
| 16 | Extensional deformation,stress relaxation and necking failure of viscoelastic filaments显示文摘 | YAO Minwu MCKINLEY G H DEBBAUT B | 1998 | Journal of Non-Newtonian Fluid Mechanics1998，79，23： | 1 |
| 17 | Extensional deformation, stress relaxation and necking failure of viscoelastic filaments 显示文摘 | YAO Minwu GARETH H BENOIT D | 1998 | J of Non-Newtonian Fluid Mech1998，79，23： | 1 |
| 18 | Perceived Interactional Justice and Trust‐in‐supervisor as Mediators for Paternalistic Leadership显示文摘 | MinWu XuHuang ChenweiLi WuLiu | 2012 | Management and Organization Review2012，，1： | 1 |
| 19 | Parameter-dependent Lyapunov functional for systems with multiple time delays显示文摘The separation of the Lyapunov matrices and system matrices plays an important role when one uses parameter-dependent Lyapunov functional handling systems with polytopic type uncertainties.The delay-dependent robust stability problem for systems with polytopic type uncertainties is discussed by using parameter-dependent Lyapunov functional.The derivative term in the derivative of Lyapunov functional is reserved and the free weighting matrices are employed to express the relationship between the terms in the system equation such that the Lyapunov matrices are not involved in any product terms with the system matrices.In addition,the relationships between the terms in the Leibniz Newton formula are also described by some free weighting matrices and some delay-dependent stability conditions are derived.Numerical examples demonstrate that the proposed criteria are more effective than the previous results. | MinWU YongHE | 2004 | 控制理论与应用(英文版)2004，2，3： | 1 |
| 20 | A single‐center retrospective analysis of liver transplantation on 255 patients with hepatocellular carcinoma显示文摘 | Zheng‐XinWang Shao‐HuaSong FeiTeng Gui‐HuaWang Wen‐YuanGuo Xiao‐MinShi JunMa You‐MinWu Guo‐ShanDing Zhi‐RenFu | 2010 | Clinical Transplantation2010，，6： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员