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| 1 | Mettl3-/Mettl14-mediated mRNA N6-methyladenosine modulates murine spermatogenesis显示文摘精子发生在双 spermatogonial 干细胞(SSC ) 哪个生产 haploid 精子期间是一个区别过程。这个高度专业化的过程精确在 transcriptional, posttranscriptional,和翻译层次被控制。这里,我们报导那 N 6-methyladenosine (m 6 一) ,调整基因表示的一个 epitranscriptomic 标记,在精子发生期间起必要作用。我们现在的全面 m 6 从五个发展阶段的老鼠 spermatogenic 房间的 mRNA methylomes:无差别的 spermatogonia,类型 A 1 spermatogonia, preleptotene spermatocytes, pachytene/diplotene spermatocytes,和圆 spermatids。m 6 有 Vasa-Cre 的 RNA methyltransferase Mettl3 或 Mettl14 引起 m 6 SSC 的 A 和弄空。m 6 为 SSC 增长 / 区别被要求的抄本的一个弄空 dysregulates 翻译。在有 Stra8-GFPCre 的先进细菌房间的 Mettl3 和 Mettl14 的联合删除破坏精子形式,而有在先进细菌房间的 Mettl3 或 Mettl14 的单个删除的老鼠显示出正常精子发生。从双异种老鼠展览的 spermatids 损害了为精子形式是必要的 haploid 特定的基因的翻译。这研究加亮 mRNA m 6 在 germline 开发的修正,潜在地保证在精子发生的不同阶段协调了翻译。 | Zhen Lin Phillip J Hsu Xudong Xing Jianhuo Fang Zhike Lu Qin Zou Ke-Jia Zhang Xiao Zhang Yuchuan Zhou Teng Zhang Youcheng Zhang Wanlu Song Guifang Jia Xuerui Yang Chuan He Ming-Han Tong | 2017 | Cell Research2017，27，10： | 61 |
| 2 | Elevation of tumor necrosis factor-α,interleukin-1β and interleukin-6 levels in aortic intima of Chinese Guizhou minipigs with streptozotocin-induced diabetes显示文摘有调停毒素的胰腺的损坏的背景大动物模型关于糖尿病 mellitus 和心血管的糖尿病的复杂并发症在研究广泛地被使用了。试图证实有 streptozotocin ( STZ )的中国贵州 minipig 模型导致了糖尿病并且描绘动物的现在的学习由在大动脉的墙中分析煽动性的 cytokine 层次当模特儿,例如肿瘤坏死因素( TNF ), interleukin-1 ( IL-1 )和 interleukin-6 ( IL-6 ) .Methods 22 男中国贵州 minipigs (变老, 4~6 个月;重量, 20 kg 到 30 kg ) 被划分成导致 STZ 的糖尿病的组(n=12 ) 和控制组(n=10 ) 。STZ (125 mg/kg ) 被管理导致多糖症,以后,胰岛素被用来在 10 mmol/L 下面控制 fasting 血葡萄糖层次。口头的葡萄糖忍耐测试(OGTT ) 以前被执行并且在 STZ 管理和丙氨酸 transaminase,天门冬素 transaminase,白朊,血脲氮, creatinine,类脂化合物和白色的浆液集中以后的一个月血房间计数以前并且六个月以后被测量。在六个月和胰为小岛房间 immunohistochemically 被检验以后,在两个组的动物是 euthanized。糖尿病的 minipigs 和控制的大动脉的 intima 在由西方的污点中等的调节的组织为 TNF- 水平被分析。在大动脉的 intima 的 TNF- , IL-1 和 IL-6 mRNA 层次是由反向的抄写和聚合酶链反应(RT-PCR ).Results 的 assayed 在浆液葡萄糖层次的重要举起被观察在 STZ 正式就职(P0.001 ) 和显著地增加的 OGTT 价值以后的一个月和六个月被注意,与基线数据相比。正常的胰有许多不规则的大小的小岛和小岛房间的小簇,当在糖尿病的 minipigs 的胰小岛房间几乎消失了时。没有统计差别以前在生物化学的考试的浆液集中被通知并且在 STZ 以后的六个月感应。西方的污点证明在 aotic intima 的戏剧性地增加的TNF-水平调节了TNF-, IL-1 和 IL-6 的中等、重要的举起 mRNA 层次被现在的学习建立了的 RT-PCR.Conclusions 揭示有 导致STZ 的 diabetes.Inflammatory cytokines 的中国贵州 minipig 模型(TNF-, IL-1 和 IL-6 )显著地在糖尿病的 minipigs 的大动脉的 intima 提高了。 | LU Lin ZHANG Qi PU Li-jin XU Xue-wei ZHANG Rui-yan ZHANG Jian-sheng HU Jian YANG Zheng-kun LU An-kang DING Feng-hua SHEN Jie CHEN Qiu-jin LOU Sheng FANG Dan-hong SHEN Wei-feng | 2007 | Chinese Medical Journal2007，，6： | 43 |
| 3 | Effects of AT1 receptor antagonist,Iosartan,on rat hepatic fibrosis induced by CCl_4显示文摘AIM To investigate effect of losartan,an AT1receptor antagonist,on hepatic fibrosis induced byCCl;and to determine whether or not AT1receptors are expressed on hepatic stellate cells,METHODS AND RESULTS Fifty male Sprague-Dawley rats,weighing(180±20)g,wererandomized into five groups(control group,modelgroup,and three losartan treated groups),inwhich all rats were given the subcutaneousinjection of 40% CCl4(every 3 days for 6 weeks)except for rats of control group.Rats of losartan-treated groups were treated with losartan(20 mg/kg,10 mg/kg,5 mg/kg,daily gavage),After 6weeks liver tissue and serum samples of all ratswere examined.Serum hyaluronic acid(HA),procollagen typeⅢ(PCⅢ)were detected byradioimmunoassays,van Giesion collagen stainingwas used to evaluate the extracellular matrix of ratswith liver fibrosis.The expression of AT1receptors,transforming growth factor-beta(TGF-β),and alpha-smooth muscle actin(a-SMA)inliver tissue were determined byimmunohistochemical techniques.Compared withmodel group,serum ALT and AST of losartan-treated groups were significantly reduced(t=4.20,P<0.01 and t=4.57,P<0.01).Serum HAand PCⅢalso had significant differences(t=3.53,P<0.01 and t=2.20,P<0.05).Thedegree of fibrosis was improved by losartan and correlated with the expressions of AT1 receptors,TGF-β,and α-SMA in liver tissue.CONCLUSION AT1 receptor antagonist,losartan,could limit the progression of the hepatic fibrosisinduced by CCl4.The mechanism may be related tothe decrease in the expression of AT1 receptorsand TGF-β,ameliorating the injury of hepatocytes;activation of local renin-angiotensin system mightrelate to hepatic fibrosis;and during progressionof fibrosis,activated hepatic stellate cells mightexpress AT1 receptors. | Hong Shan Wei Ding Guo Li Han Ming Lu Yu Tao Zhan Zhi Rong Wang Xin Huang Jing Zhang Ji Lin Cheng Qin Fang Xu Department of Gastroenterology,Xinhua Hospital,Shanghai Second Medical University,Shanghai 200092,China | 2000 | World Journal of Gastroenterology2000，6，4： | 42 |
| 4 | Biochemical characteristics of neonatal cholestasis induced by citrin deficiency显示文摘AIM:To explore differences in biochemical indices between neonatal intrahepatic cholestasis caused by citrin deficiency (NICCD) and that with other etiologies. METHODS:Patients under 6 mo of age who were referred for investigation of conjugated hyperbiliru-binaemia from June 2003 to December 2010 were eligible for this study. After excluding diseases affecting the extrahepatic biliary system, all patients were screened for the two most common SLC25A13 mutations; the coding exons of the entire SLC25A13 gene was sequenced and Western blotting of citrin protein performed in selected cases. Patients in whom homo-zygous or compound heterozygous SLC25A13 mutation and/or absence of normal citrin protein was detected were defined as having NICCD. Cases in which no specific etiological factor could be ascertained after a com-prehensive conjugated hyperbilirubinaemia work-up were defined as idiopathic neonatal cholestasis (INC). Thirty-two NICCD patients, 250 INC patients, and 39 infants with cholangiography-confirmed biliary atresia (BA) were enrolled. Laboratory values at their first visit were abstracted from medical files and compared. RESULTS:Compared with BA and INC patients, the NICCD patients had significantly higher levels of total bile acid (TBA) [all measures are expressed as median (inter-quartile range):178.0 (111.2-236.4) μmol/L in NICCD vs 112.0 (84.9-153.9) μmol/L in BA and 103.0 (70.9-135.3) μmol/L in INC, P = 0.0001]. The NICCD patients had significantly lower direct bilirubin [D-Bil 59.6 (43.1-90.9) μmol/L in NICCD vs 134.0 (115.9-151.2) μmol/L in BA and 87.3 (63.0-123.6) μmol/L in INC, P = 0.0001]; alanine aminotransferase [ALT 34.0 (23.0-55.0) U/L in NICCD vs 108.0 (62.0-199.0) U/L in BA and 84.5 (46.0-166.0) U/L in INC, P = 0.0001]; aspartate aminotransferase [AST 74.0 (53.5-150.0) U/L in NICCD vs 153.0 (115.0-239.0) U/L in BA and 130.5 (81.0-223.0) U/L in INC, P = 0.0006]; albumin [34.9 (30.7-38.2) g/L in NICCD vs 38.4 (36.3-42.2) g/L in BA and 39.9 (37.0-42.3) g/L in INC, P = 0.0001]; glucose [3.2 (2.0-4.4) mmol/L in NICCD vs 4.1 (3.4-5.1) mmol/L in BA and 4.0 (3.4-4.6) mmol/L in INC, P = 0.0014] and total cholesterol [TCH 3.33 (2.97-4.00) mmol/L in N ICCD vs 4.57 (3.81-5.26) mmol/L in BA and 4.00 (3.24-4.74) mmol/L in INC, P = 0.0155] levels. The D-Bil to total bilirubin (T-Bil) ratio was significantly lower in NICCD patients [all measures are expressed as median (inter-quartile range):0.54 (0.40-0.74)] than that in BA patients [0.77 (0.72-0.81), P = 0.001] and that in INC patients [0.74 (0.59-0.80), P = 0.0045]. A much higher AST/ALT ratio was found in NICCD patients [2.46 (1.95-3.63)] compared to BA patients [1.38 (0.94-1.97), P = 0.0001] and INC patients [1.48 (1.10-2.26), P = 0.0001]. NICCD patients had significantly higher TBA/D-Bil ratio [3.36 (1.98-4.43) vs 0.85 (0.72-1.09) in BA patients and 1.04 (0.92-1.14) in INC patients, P = 0.0001], and TBA/TCH ratio [60.7 (32.4-70.9) vs 24.7 (19.8-30.2) in BA patients and 24.2 (21.4-26.9) in INC patients, P = 0.0001] compared to the BA and INC groups. CONCLUSION:NICCD has significantly different bio- chemical indices from BA or INC. TBA excretion in NICCD appeared to be more severely disturbed than that of bilirubin and cholesterol. | Jian-She Wang Xiao-Hong Wang Ying-Jie Zheng Hai-Yan Fu Rui Chen Yi Lu Ling-Juan Fang Takeyori Saheki Keiko Kobayashi | 2012 | World Journal of Gastroenterology2012，18，39： | 35 |
| 5 | Population Change and Resulting Slowdown in Potential GDP Growth in China显示文摘As a result of the shrinking working age population(aged 15 to 59 years),all factors that have driven China's rapid economic growth over the past 30 years tend to diminish from2010.The present paper estimates the average annual growth rate of potential output to be7.2percent over the 12th Five-year Plan period and 6.1 percent over the 13th Five-year Plan period.Future sustainable growth requires furthering economic reform in related areas to enhance potential growth.This paper simulates two scenarios in which both an increase in labor force participation and improvement in total factor productivity can significantly enhance the potential GDP growth rate.Relevant policy implications are discussed. | Fang Cai Yang Lu | 2013 | China & World Economy2013，21，2： | 40 |
| 6 | Design and engineering implementation of non-supplementary fired compressed air energy storage system: TICC-500显示文摘The integration and accommodation of the wind and solar energy pose great challenges on today’s power system operation due to the intermittent nature and volatility of the wind and solar resources.High efficient large-scale electrical energy storage is one of the most effective and economical solutions to those problems.After the comprehensive review of the existing storage technologies,this paper proposes an overall design scheme for the Non-supplementary Fired Compressed Air Energy Storage(NFCAES)system,including system design,modeling and efficiency assessment,as well as protection and control.Especially,the design principles of the multistage regenerative,i.e.heat recovery system which is used to fully recycle and utilize the waste heat from compression are provided,so as the overall system efficiency evaluation method.This paper theoretically ascertains the storage decoupling rules in the potential and internal energy of molecular compressed air and reveals the conversion mechanism of gas,heat,power,electricity and other forms of energy.On this basis,a 500-k W physical simulation system of CAES system(TICC-500,Tsinghua-IPCCAS-CEPRI-CAES)is built,which passed a system-wide 420-k W load power generation test with less pollution and zero carbon emissions.Besides,the multi-form energy conversion of multi-stage regenerative CAES and storage efficiency is verified,especially its incomparable superiority in solving the uncertainty problem in wind and solar power generation.Finally,the propaganda and application scenario of the CAES system in China is introduced. | MEI Sheng Wei WANG Jun Jie TIAN Fang CHEN Lai Jun XUE Xiao Dai LU Qiang ZHOU Yuan ZHOU Xiao Xin | 2015 | Science China(Technological Sciences)2015，58，4： | 37 |
| 7 | Systemic inflammatory response following acute myocardial infarction显示文摘在 infarcted 心的尖锐 cardiomyocyte 坏死产生联系损坏的分子的模式,激活表明,并且被触发强烈煽动性的回答的补充和像使用费的 receptor/interleukin-1。Inflammasomes 也认出危险信号并且调停跟随尖锐心肌的梗塞(AMI ) 的无菌的煽动性的反应。煽动性的反应服务修理心,但是过多的发炎导致不利左室的改变和心失败。除了局部炎症,深刻全身的发炎反应与 AMI 在病人被记录了,它包括传播煽动性的 cytokines, chemokines 和房间的举起外部白血球和血小板的粘附分子,和激活。过多的煽动性的反应能被一个 deregulated 免疫系统引起。AMI 也与骨头髓激活和怒气 monocytopoiesis 被联系,它在发炎的地点支撑单核白血球的连续供应。积累的证据证明了那全身的发炎加重动脉粥样硬化,为全身的发炎的标记是不利临床的结果的预言者(例如死亡,周期性的心肌的梗塞,和心失败) 在有 AMI 的病人。 | Lu FANG Xiao-Lei Moorea Anthony M Dart Le-Min WANG | 2015 | Journal of Geriatric Cardiology2015，12，3： | 36 |
| 8 | Digital medical technology based on 64-slice computed tomography in hepatic surgery显示文摘有计算机技术的快速的开发的背景,数字药在外科成为了一个新方向。在肝的外科的数字药的申请仍然在在文学报导的早阶段和更少。这研究的目的是在肝的外科的上下文使用数字医药技术。从 64 片的方法数据 17 个病人的螺旋状的计算断层摄影术,与 hepatocellular 癌包括 13 并且 4 与肝的 hemangioma,被进口进独立地发达的医药图象软件节目,分割和三维的重建被执行。三维的模型然后与形式自由的建模系统被处理。我们使用了虚拟外科的仪器在模型上执行外科。模仿的手术包括了六肝的 segmentectomies,四左 hemihepatectomies,为 hepatocellular 癌的三正确 hemihepatectomies,一肝的 segmentectomy,二脱衣手术,和不规则的 segmentectomy 与为 hemangioma 剥去外科相结合的 segmentectomies。为包含超过三个肝的片断,总数和剩余的切除术,功能的肝的体积在模拟外科前后被测量,并且切除术比率是模型的解剖是的 calculated.Results 不同并且被用来本地化损害。我们使用了虚拟外科的仪器执行模仿的手术并且使用了模型优化实际手术。我们能象数字医药技术是的外科的 risk.Conclusions 一样最小化切除术体积在肝的疾病的诊断并且在优化外科的计划有用。三维的模型能减少外科的风险和帮助阻止手术后的肝的失败。 | FANG Chi-hua HUANG Yan-peng CHEN Mian-ling LU Chao-min LI Xiao-feng QIU Wen-feng | 2010 | Chinese Medical Journal2010，，9： | 29 |
| 9 | 5-Hydroxymethylcytosine signatures in circulating cell-free DNA as diagnostic biomarkers for human cancers显示文摘象 5-methylcytosine (5mC ) 和 5-hydroxymethylcytosine (5hmC ) 那样的 DNA 修正是知道在哺乳动物影响全球基因表示的 epigenetic 标记。在人的染色体,与基因表示的靠近的关联和高化学的稳定性给他们的流行,这些 DNA epigenetic 标记能为癌症用作理想的 biomarkers 诊断。利用一种高度敏感、选择的化学标记技术,我们这里报导在传播没有房间的 DNA ( cfDNA )并且在从最近与 colorectal 诊断的 260 个病人的一个队收集的配对的肿瘤和邻近的纸巾的 genomic DNA ( gDNA )的 5hmC 的染色体宽的介绍,胃,胰腺,从 90 个健康个人的肝或甲状腺癌症和正常纸巾。5hmC 主要在与开的染色质和容许的 histone 修正重合的 transcriptionally 活跃的区域被散布。柔韧的联系癌症的 5hmC 签名在 cfDNA 被识别为特定的癌症类型是特征的。传播 cfDNA 的 5hmC 底 biomarkers colorectal 和胃的癌症是高度预兆的并且比常规 biomarkers 优异并且比得上从织物活体检视的 5hmC biomarkers。因此,这新策略能从血样品的分析为癌症的诊断和预后导致有效、最低限度地侵略的方法的发展。 | Wenshuai Li Xu Zhang Xingyu Lu Lei You Yanqun Song Zhongguang Luo Jun Zhang Ji Nie Wanwei Zheng Diannan Xu Yaping Wang Yuanqiang Dong Shulin Yu Jun Hong Jianping Shi Hankun Hao Fen Luo Luchun Hua Peng Wang Xiaoping Qian Fang Yuan Lianhuan Wei Ming Cui Taiping Zhang Quan Liao Menghua Dai Ziwen Liu Ge Chen Katherine Meckel Sarbani Adhikari Guifang Jia Marc B Bissonnette Xinxiang Zhang Yupei Zhao Wei Zhang Chuan He Jie Liu | 2017 | Cell Research2017，27，10： | 30 |
| 10 | Efficacy and safety of a novel anti-HER2 therapeutic antibody RC48 in patients with HER2-overexpressing,locally advanced or metastatic gastric or gastroesophageal junction cancer:a single-arm phase II study显示文摘Background:Current treatment options for human epidermal growth factor receptor 2(HER2)-overexpressing gastric cancer at third-line have shown limited clinical benefit.Further,there is no specific treatment for HER2 immunohistochemistry(IHC)2+and fluorescence in-situ hybridization-negative patients.Here,we report the efficacy and safety of a novel anti-HER2 antibody RC48 for patients with HER2-overexpressing,advanced gastric or gastroesophageal junction cancer.Methods:Patients with HER2-overexpressing(IHC 2+or 3+),locally advanced or metastatic gastric or gastroesophageal junction cancer who were under at least second-line therapy were eligible and received RC482.5 mg/kg alone every 2 weeks.The primary endpoint was the objective response rate(ORR)assessed by an independent review committee.Secondary endpoints included progressionfree survival(PFS),overall survival(OS),duration of response,time to progression,disease control rate,and safety.Results:Of 179 patients screened,125 were eligible and received RC48 treatment.The ORR was 24.8%(95%confidence interval[CI]:17.5%-33.3%).The median PFS and OS were 4.1 months(95%CI:3.7-4.9 months)and 7.9 months(95%CI:6.7-9.9 months),respectively.The most frequently reported adverse events were decreased white blood cell count(53.6%),asthenia(53.6%),hair loss(53.6%),decreased neutrophil count(52.0%),anemia(49.6%),and increased aspartate aminotransferase level(43.2%).Serious adverse events(SAEs)occurred in 45(36.0%)patients,and RC48-related SAEs were mainly decreased neutrophil count(3.2%).Seven patients had adverse events that led to death were not RC48-related.Conclusions:RC48 showed promising activity with manageable safety,suggesting potential application in patients with HER2-overexpressing,advanced gastric or gastroesophageal junction cancer who have previously received at least two lines of chemotherapy. | Zhi Peng Tianshu Liu Jia Wei Airong Wang Yifu He Liuzhong Yang Xizhi Zhang Nanfeng Fan Suxia Luo Zhen Li Kangsheng Gu Jianwei Lu Jianming Xu Qingxia Fan Ruihua Xu Liangming Zhang Enxiao Li Yuping Sun Guohua Yu Chunmei Bai Yong Liu Jiangzheng Zeng Jieer Ying Xinjun Liang Nong Xu Chao Gao Yongqian Shu Dong Ma Guanghai Dai Shengmian Li Ting Deng Yuehong Cui Jianmin Fang Yi Ba Lin Shen | 2021 | Cancer Communications2021，41，11： | 29 |
| 11 | Effect of lifestyle intervention on non-alcoholic fatty liver disease in Chinese obese children显示文摘AIM: To investigate the effect of lifestyle intervention on non-alcoholic fatty liver disease (NAFLD) in Chinese obese children. METHODS: Seventy-six obese children aged from 10 to 17 years with NAFLD were enrolled for a one-month intervention and divided randomly into three groups. Group1, consisting of 38 obese children, was an untreated control group without any intervention. Group 2, consisting of 19 obese children in summer camp, was strictly controlled only by life style intervention. Group 3, consisting of 19 obese children, received oral vitamin E therapy at a dose of 100 mg/d. The height, weight, fasting blood glucose (FBG), fasting serum insulin (FINS), plasma alanine aminotransferase (ALT), aspartate aminotransferase (AST), triglyceride (TG), total cholesterol (TCHO) and homeostasis model assent- insulin resistance (HOMA-IR) were measured at baseline and after one month. All patients were underwent to an ultrasonographic study of the liver performed by one operator who was blinded to the groups. RESULTS: The monitor indices of BMI, ALT, AST, TG, TCHO and HOMA-IR were successfully improved except in group 1. BMI and ALT in group 2 were reduced more significantly than in group 3 (2.44 ± 0.82 vs 1.45 ± 0.80, P = 0.001; 88.58 ± 39.99 vs 63.69 ± 27.05, P = 0.040, respectively).CONCLUSION: Both a short-term lifestyle intervention and vitamin E therapy have an effect on NAFLD in obese children. Compared with vitamin E, lifestyle intervention is more effective. Therefore, lifestyle intervention should represent the first step in the management of children with NAFLD. | Chun-Lin Wang Li Liang Jun-Fen Fu Chao-Chun Zou Fang Hong Jin-Zheng Xue Jin-Rui Lu Xiang-Min Wu | 2008 | World Journal of Gastroenterology2008，14，10： | 28 |
| 12 | The regulatory role of AT 1 receptor on activated HSCs in hepat,c fibrogenesis,effects of RAS inhibitors on hepatic fibrosis induced by CCl_4显示文摘AIM To assess the effect of ACE inhibitor andAng Ⅱ type Ⅰ(AT1)receptor antagonist inpreventing hepatic fibrosis caused by CCl4administration in rats;to investigate whether ornot there are expression of AT 1 receptors onhepatic stellate cells;and to observe the effectof Ang Ⅱ on proliferation and ECM synthesis ofcultured HSCs.METHODS Studies were conducted in maleSprague-Dawley rats.Except for thehepatofibrotic model group and the controlgroup,in three treated groups,either enalapril(5 mg/kg),or Iosartan(10 mg/kg),or enalapril+Iosartan were given to the fibrotic rats bydaily gavage,and saline vehicle was given tomodel and normal control rats.After 6 weeks,liver fibrosis was assessed directly by hepaticmorphometric analysis,which has beenconsidered the gold standard for thequantification of fibrosis.The expressions of AT1 receptors and(α-mooth muscle actin,α-SMA)in liver tissue or isolated hepatic stellate cells(HSCs)were detected by immunohistochemicaltechniques.The effect of Ang Ⅱ on HSCproliferation was determined by MTT method.Effect of Ang Ⅱ on collagen synthesis of HSCswas determined by 3H-proline incorporation.RESULTS Contrasted to the fibrosis in rats ofthe model group,groups of rats treated with either enalapril or Iosartan,or a combination oftwo drugs showed a limited expansion of theinterstitium(4.23±3.70 vs 11.22±4.79,P<0.05),but no difference was observedamong three treated groups(5.38±3.43,4.96±2.96,4.23±2.70,P>0.05).Expression of AT 1receptors was found in fibrotic interstitium offibrotic rats,whereas in normal control rats theywere limited to vasculature only to a very slightdegree.AT 1 receptors were also expressed onactivated HSCs in the culture.At concentrationsfrom 10-9to 10-5mol/L,Ang Ⅱ stimulated HSCproliferation in culture in a dose-dependentmanner.Increasing Ang Ⅱ concentrationsproduced corresponding increases in 3H-prolineincorporation.Differences among groups were significant.CONCLUSION Angiotensin-converting enzyme inhibitors and AT I blocker may slow the progression of hepatic fibrosis; activated HSCs express AT 1 receptors, and Ang Ⅱ can stimulate the proliferation and collagen synthesis of HSCs in a dose-dependent manner; and activation of RAS may be related to hepatic fibrogenesis induced by CCI4. | Hong Shan Wei Han Ming Lu Ding Guo Li Yu Tao Zhan Zhi Rong Wang Xin Huang Ji Lin Cheng Qin Fang Xu Department of Gastroenterology,Xinhua Hospital,Shanghai Second Medical University,Shanghai 200092,China | 2000 | World Journal of Gastroenterology2000，6，6： | 27 |
| 13 | Magnifying narrow-band imaging endoscopy is superior in diagnosis of early gastric cancer显示文摘AIM:To evaluate the diagnostic effectiveness of white light endoscopy,magnifying endoscopy(ME),and magnifying narrow-band imaging endoscopy(ME-NBI) in detecting early gastric cancer(EGC).METHODS:From March 2010 to June 2012,a total of 3616 patients received screening for gastric cancer by magnifying endoscopy. There were 3675 focal gastric lesions detected using conventional high definition white light endoscopy(HD-WLE) in four different referentialhospitals that were recruited for further investigation using ME and ME-NBI. The images obtained from HD-WLE,ME,and ME-NBI were reviewed by four experienced endoscopists to evaluate their diagnostic effectiveness for EGC. The diagnosis of cancerous and non-cancerous lesions was conducted by evaluating the microvascular and microsurface patterns using the VS classification system. The final endoscopic diagnosis of each lesion was determined by consultation when a disagreement occurred. We used histopathological results as the gold standard for the diagnosis of EGC.RESULTS:Among the 3675 lesions found,1508 were validated by pathological findings as chronic gastritis,1279 as chronic gastritis with intestinal metaplasia,631 as low-grade neoplasia,and 257 as EGC. The sensitivity,specificity,positive predictive value,negative predictive value,and accuracy of HD-WLE for the diagnosis of EGC were 71.2%,99.1%,85.5%,97.9% and 97.1%,respectively. The results of ME for diagnosing EGC were 81.3%,98.8%,83.3%,98.6% and 97.6%,respectively. The results of ME-NBI for the diagnosis of EGC were 87.2%,98.6%,82.1%,99.0% and 97.8%,respectively. The diagnostic sensitivity and accuracy of paired ME and ME-NBI were significantly better than those of HD-WLE(P < 0.05).CONCLUSION:HD-WLE has a relatively high accuracy for diagnosing EGC and is an effective screening tool. Further investigations of ME and ME-NBI are required to achieve superior accuracy. | Hang Yu Ai-Ming Yang Xing-Hua Lu Wei-Xun Zhou Fang Yao Gui-Jun Fei Tao Guo Li-Qing Yao Li-Ping He Bang-Mao Wang | 2015 | World Journal of Gastroenterology2015，21，30： | 26 |
| 14 | PTEN-L is a novel protein phosphatase for ubiquitin dephosphorylation to inhibit PINK1-Parkin-mediated mitophagy显示文摘Mitophagy 是为损坏线粒体的特定的消除的选择 autophagy 的一种重要类型。(PINK1 )导致 PTEN 的通常认为的 kinase 蛋白质 1 催化 ubiquitin (Ub ) 的 phosphorylation 在 PINK1-Parkin-mediated mitophagy 的发作起一个关键作用。(PTEN-L ) 磷酸酶和 tensin 相当或相同的事物(PTEN ) 长是 PTEN 的最新识别的 isoform,与到它的 N 终点的 173 氨基酸的增加。这里,我们报导 PTEN-L 是经由它对 phosphorylated ubiquitin 的蛋白质磷酸酶活动的 mitophagy 的一个新奇否定管理者。我们发现 PTEN-L 在外部 mitochondrial 膜(OMM ) 本地化,而 PTEN-L 的删除支持, PTEN-L 的 overexpression 禁止, mitophagy 由各种各样的损坏线粒体的代理人导致了。机械学地, PTEN-L 能够有效地阻止 Parkin mitochondrial translocation,减少 Parkin phosphorylation,维持它的关上的不活跃的符合构造,并且禁止它的 E3 ligase 活动。更重要地, PTEN-L 在 vivo 减少 phosphorylated ubiquitin (pSer65-Ub ) 的水平,并且在 vitro,磷酸酶试金经由它的蛋白质磷酸酶活动证实那 PTEN-L dephosphorylates pSer65-Ub,独立于它的类脂化合物磷酸酶功能。一起拿,我们的调查结果为 ubiquitin 作为蛋白质磷酸酶表明 PTEN-L 的新奇功能,它抵抗在 mitophagy 正式就职和 mitophagy 的最终的抑制导致前馈控制机制的阻塞的调停 PINK1 的 ubiquitin phosphorylation。因此,理解 PTEN-L 的这新奇功能在控制 mitophagy 的分子的难题提供一关键错过片,在包括象 Parkinsons 那样的 neurodegenerative 混乱的许多重要人的疾病的一个批评过程疾病。 | Liming Wang Yik-Lam Cho Yancheng Tang Jigang Wang Jung-Eun Park Yajun Wu Chunxin Wang Yan Tong Ritu Chawla Jianbin Zhang Yin Shi Shuo Deng Guang Lu Yihua Wu Hayden Weng-Siong Tan Pornteera Pawijit Grace Gui-Yin Lim Hui-Ying Chan Jingzi Zhang Lei Fang Hanry Yu Yih-Cherng Liou Mallilankaraman Karthik Boon-Huat Bay Kah-Leong Lim Siu-Kwan Sze Celestial T. Yap Han-Ming Shen | 2018 | Cell Research2018，28，8： | 25 |
| 15 | Berberine attenuates ischemia-reperfusion injury through inhibiting HMGB 1 release and NF-κB nuclear translocation显示文摘煽动性的损坏在服的 ischemic 致病起一个重要作用并且为中风的治疗代表一个新目标。Berberine 是有多重有益的生物活动的自然的药。在这研究,我们探索了在使遭到的老鼠位于 berberine 的 neuroprotective 行动下面的机制短暂中间的服的动脉吸藏(tMCAO ) 。雄的老鼠被管理 berberine (25, 50mg/kg/d, intragastric;i.g ) , glycyrrhizin (50mg/kg/d, intraperitoneal ) ,或 berberine (50mg/kg/d, i.g ) 正 glycyrrhizin (50mg/kg/d, intraperitoneal ) 为在 tMCAO 前的 14 连续的天。神经病学的赤字分数在 tMCAO 以后在 24h 被评估,然后老鼠被打死获得大脑样品。我们证明有 berberine 的那个预告的处理 dose-dependently 减少了在浆液和 ischemic 的梗塞尺寸,神经病学的赤字, hispathological 变化,大脑浮肿,和煽动性的调停人外皮的织物。我们表明有 berberine 的那个预告的处理显著地提高了 18 ischemic 半球在击以后在 24h 与车辆组作比较的 F-fluorodeoxyglucose。而且,有 berberine 的预告的处理 dose-dependently 压制了高活动性的组 box1 (HMGB1 ) 的原子 cytosolic translocation 蛋白质, cytosolic 原子因素 kappa B (NF-B ) 的原子 translocation 并且减少在 ischemic 的 TLR4 的表示外皮的织物。而且, glycyrrhizin 和 berberine 的合作管理比独自管理的 berberine 或 glycyrrhizin 在 HMGB1/TLR4/NF-B 小径上施加了更多的有势力抑制。这些结果证明 berberine 保护大脑免于 ischemia-reperfusion 损害,机制可以依靠它的反煽动性的效果由压制 HMGB1/TLR4/NF-B 发信号的激活调停了。 | Jun-rong Zhu Hal-dan Lu Chao Guo Wei-rong Fang Hong-dong Zhao Jun-shan Zhou Feng Wang Yan-li Zhao Yun-man Li Ying-dong Zhang Chang-qing Yang Jian-guo Sun | 2018 | Acta Pharmacologica Sinica2018，39，11： | 24 |
| 16 | Ten cities cross-sectional questionnaire survey of children asthma and other allergies in China显示文摘Asthma,rhinitis and eczema(allergic or non-allergic)have increased throughout the world during the last decades,especially among children.Changes in the indoor environment are suspected to be important causes.China has experienced a dramatic change in indoor environmental exposures during the past two decades.However,such changes and their associations with children’s asthma and other health aspects have not been thoroughly studied.China,Children,Homes,Health(CCHH),Phase I,was a cross-sectional questionnaire survey of 48219 children 1–8 years old in 10 Chinese cities during 2010–2012.The questionnaire includes the International Study of Asthma and Allergies in Childhood(ISAAC)core health questions and additional questions regarding housing,life habits and outdoor environment.In health analyses,children aged 3–6 years old were included.The prevalences of doctor diagnosed asthma varied from 1.7%to 9.8%(mean 6.8%),a large increase from 0.91%in 1999 and 1.50%in2000.The prevalence of wheeze,rhinitis and atopic eczema(last 12 months)varied from 13.9%to 23.7%,24.0%to 50.8%and4.8%to 15.8%,respectively.Taiyuan had the lowest prevalences of all illnesses and Shanghai the highest,except for wheezewhere the highest value was for Urumqi.We found(1)no obvious association between disease prevalences and ambient PM10concentrations and(2)higher prevalences of disease in humid climates with hot summers and cold winters,but with no centrally heated buildings.Associations between the diseases and economic status as indexed by Gross Domestic Product(GDP)requires further study. | ZHANG YinPing LI BaiZhan HUANG Chen YANG Xu QIAN Hua DENG QiHong ZHAO ZhuoHui LI AnGui ZHAO JiaNing ZHANG Xin QU Fang HU Yu YANG Qin WANG Juan ZHANG Ming WANG Fang ZHENG XiaoHong LU Chan LIU ZhiJian SUN YueXia MO JinHan ZHAO YiLi LIU Wei WANG TingTing NORBCK Dan BORNEHAG Carl-Gustaf SUNDELL Jan | 2013 | Chinese Science Bulletin2013，58，34： | 22 |
| 17 | Charlson comorbidity index helps predict the risk of mortality for patients with type 2 diabetic nephropathy显示文摘研究目的:探讨Charlson合并症指数(CCI)对2型糖尿病肾病死亡率的预测作用。研究方法:建立2型糖尿病肾病研究队列,根据CCI评分将患者合并症的严重程度分为三度:轻度(CCI1–2分)、中度(CCI 3–4分)、重度(CCI≥5分)。将影响死亡率的因素及按CCI分层后的组间差异指标进行Logistic回归分析及方差分析(ANOVA)。Kaplan-Meier生存曲线分析CCI指数对生存时间及死亡率的影响。重要结论:533例2型糖尿病肾病患者纳入研究。所有患者的CCI评分均大于1,44.7%(238/533)的患者死亡。患者的死亡率随CCI评分增加而增加,CCI 1–2分患者的死亡率为21.0%(50/238),CCI 3–4分患者的死亡率为56.7%(135/238),CCI≥5分患者的死亡率为22.3%(53/238)。Logistic回归分析显示CCI评分、血红蛋白和血浆白蛋白水平是患者死亡率的预测因子(P<0.05)。方差分析结果显示,与CCI评分相对较低的患者相比,CCI评分越高的患者其血红蛋白水平较低,而血清肌酐较高,死亡率也更高。Kaplan-Meier生存曲线显示CCI评分越高的患者生存时间越短。CCI评分是一种简单易行且实用的评估疾病合并症的方法,可用于预测2型糖尿病肾病患者的死亡率。关注2型糖尿病肾病患者的合并症将有利于早期、有效治疗及改善预后。 | You-qun HUANG Rong GOU Yong-shu DIAO Qing-hua YIN Wen-xing FAN Ya-ping LIANG Yi CHEN Min WU Li ZANG Ling LI Jing ZANG Lu CHENG Ping FU Fang LIU | 2014 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2014，15，1： | 23 |
| 18 | Crustal structure of northeastern margin of the Tibetan Plateau by receiver function inversion显示文摘Using seismic data of about one year recorded by 18 broadband stations of ASCENT project,we obtained 2547 receiver functions in the northeastern Tibetan Plateau.The Moho depths under 14 stations were calculated by applying the H-κ domain search algorithm.The Moho depths under the stations with lower signal-noise ratio(SNR) were estimated by the time delay of the PS conversion.Results show that the Moho depth varies in a range of ~40–60 km.The Moho near the Haiyuan fault is vague,and its depth is larger than those on its two sides.In the Qinling-Qilian Block,the Moho becomes shallower gradually from west to east.To the east of 105°E,the average depth of the Moho is 45 km,whereas the west is 50 km or even deeper.Combining our results with surface wave research,we suggest a boundary between the Qinling and the Qilian Mountains at around 105°E.S wave velocities beneath 15 stations have been obtained through a linear inversion by using Crust2.0 as an initial model,and the crustal thickness that was derived by H-κ domain search algorithm was also taken into account.The results are very similar to the results of previous active source studies.The resulting figure indicates that low velocity layers developed in the middle and lower crust beneath the transition zone of the Tibet Block and western Qinling,which may be related to regional faults and deep earth dynamics.The velocity of the middle and lower crust increases from the Songpan Block to the northeastern margin of Tibetan Plateau.Based on the velocity of the crust,the distribution of the low velocity zone and the composition of the curst(Poisson's ratio),we infer that the crust thickening results from the crust shortening along the direction of compression. | LIU QiMin ZHAO JunMeng LU Fang LIU HongBing | 2014 | Science China Earth Sciences2014，57，4： | 23 |
| 19 | Clinical and experimental study on regional administration of phosphorus32 glassmicrospheres in treating hepatic carcinoma显示文摘AIM To study the therapeutical effectiveness, dosage range and toxic adverse effects of domestic phosphorus 32 glass microsphere and evaluate its clinical significance. METHODS Ⅰ. Fifty two BALB/*!c tumor bearing male nude mice were allocated into treatment group( n =38) and control group( n =14). In the former group different doses of 32 P GMS were injected into the tumor mass, while in the latter 31 P GMS or no treatment was given. The experimental animals were sacrificed in batches, and then the tumors and their nearby tissues were examined by light and electron microscopy. Ⅱ. Through selective catheterization of hepatic artery, 32 P GMS was infused to 5 healthy domestic pigs in a dosage equivalent to the therapeutic dose for human being, and 31 P GMS was infused to another 5 healthy domestic pigs. Two pigs infused with contrast medium served as whole course blank controls. One pig from each group was surrendered to euthanasia at week 1, 4, 8 and 16 respectively. The ultrastructural histopath ological changes in liver tissues taken from different sites were evaluated semiquan titatively. Ⅲ. One hundred and twenty seven times of 32 P GMS intrahepatic artery interventional therapies were performed on 93 patients with hepatic carcinoma, including 79 cases of primary hepatic carcinoma and 14 cases of secondary hepatic carcinoma. 32 P GMS ( n =30), and group B, 32 P GMS and half dose of trans hepatic artery embolization (TAE) ( n =49) , and 18 patients with HCC by TAE only as control group C. Fourteen patients with secondary hepatic carcinoma were treated in the same way as group B or C. RESULTS Ⅰ. Comparing with the control group, the treatment group of tumor bearing nude mice attained the tumor inhibition rates of 59 7%-93 7% ( F =579 62, P <0 01) at 14*!d . At an absorbed dose of 7320Gy, the tumor cells were completely destroyed. When the absorbed doses ranged from 1830Gy to 3660Gy, most of the tumor cells showed the evidences of injury or necrosis, but there appeared some well differentiated tumor cells and enhanced effect of the autoimmunocytes. At an absorbed dose of 366Gy or less, some tumor cells still remained active proliferative ability. The definite anticancer effect appeared as early as 3d after intratumoral injection of 32 P GMS. Ⅱ. The cumulative amount of 32 P GMS in the target tissue after trans hepatic artery instillation attained more than 90% of the total dose administrated. Semiquantitative analysis of ultrastructral morphology in the experimental group showed no statistical difference between the nuclear abnormality (N abn ) and mitochondrial variability (M var ) at week 1 or 2, but revealed prominent difference (χ 2=6 70-9 68, P <0 01 , χ 2=65 09-115 09, P <0 001 ) as compared with those in the other groups. In the experimental group the N abn in tissues showed no significant difference between week 8 and week 16. No apparent changes were found in the stomach, spleen, kidney and lung tissues of the experimental pigs. Ⅲ. The therapeutical results of HCC patients in group A were closely approximated to those of group C, no hematological toxic side effects were noted, and the systemic reaction was mild. In some patients 2*!mos - 3*!mos after treatment some secondary foci appeared around the periphery of the primary lesion. In general better effectiveness was obtained in patients with small lesion. After analyzing by RIDIT method, the therapeutic result in group B was significantly better than that in group C, and secondary foci around the original lesion were rarely seen at 3*!mos after treatment. In group C the collateral circulation was reestablished along the periphery of primary foci and the secondary foci appeared more frequently, and required to undergo several courses of treatment. In group B, 4 cases of HCC were treated surgically as their mass decreased in size after 32 P GMS treatment. | LIU Lu, JIANG Zao, TENG Gao Jun, SONG Ji Zhi, ZHANG Dong Sheng, GUO Qing Ming, FANG Wen, HE Shi Cheng and GUO Jin He | 1999 | World Journal of Gastroenterology1999，5，6： | 21 |
| 20 | Microbubble-enhanced ultrasound exposure improves gene transfer in vascular endothelial cells显示文摘AIM: To explore the effects of ultrasound exposure combined with microbubble contrast agent (SonoVue) on the permeability of the cellular membrane and on the expression of plasmid DNA encoding enhanced green fluorescent protein (pEGFP) transfer into human umbilical vein endothelial cells (HUVECs). METHODS: HUVECs with fluorescein isothiocyanate- dextran (FD500) and HUVECs with pEGFP were exposed to continuous wave (1.9 MHz, 80.0 mW/cm2) for 5 min, with or without a SonoVue. The percentage of FD500 taken by the HUVECs and the transient expression rate of pEGFP in the HUVECs were examined by fluorescence microscopy and flow cytometry, respectively. RESULTS: The percentage of FD500-positive HUVECs in the group of ultrasound exposure combined with SonoVue was significantly higher than that of the group of ultrasound exposure alone (24.0% ± 5.5% vs 66.6% ± 4.1%, P < 0.001). Compared with the group of ultrasound exposure alone, the transfection expression rate of pEGFP in HUVECs was markedly increased with the addition of SonoVue (16.1% ± 1.9% vs 1.5% ± 0.2%, P < 0.001). No statistical significant difference was observed in the HUVECs survival rates between the ultrasound group with and without the addition of SonoVue (94.1% ± 2.3% vs 91.1% ± 4.1%).CONCLUSION: The cell membrane permeability of HUVECs and the transfection efficiency of pEGFP into HUVECs exposed to ultrasound are significantly increased after addition of an ultrasound contrast agent withoutobvious damage to the survival of HUVECs. This non- invasive gene transfer method may be a useful tool for clinical gene therapy of hepatic tumors. | Fang Nie Hui-Xiong Xu Qing Tang Ming-De Lu | 2006 | World Journal of Gastroenterology2006，12，46： | 21 |
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