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| 1 | Isolation and characterization of H7N9 viruses from live poultry markets--Implication of the source of current H7N9 infection in humans显示文摘On March 31, 2013, the National Health and Family Planning Commission announced that human infections with a previously undescribed influenza A (H7N9) virus had occurred in Shanghai and Anhui Province, China. To investigate the possible origins of the H7N9 viruses causing these human infections, we collected 970 samples, including drinking water, soil, and cloacal and tracheal swabs of poultry from live poultry markets and poultry farms in Shanghai and Anhui Province. Twenty samples were positive for the H7N9 influenza virus. Notably, all 20 viruses were isolated from samples collected from live poultry markets in Shanghai. Phylogenetic analyses showed that the six internal genes of these novel human H7N9 viruses were derived from avian H9N2 viruses, but the ancestor of their HA and NA genes is uncertain. When we examined the phylogenetic relationship between the H7N9 isolates from live poultry markets and the viruses that caused the human infections, we found that they shared high homology across all eight gene segments. We thus identified the direct avian origin of the H7N9 influenza viruses that caused the human infections. Importantly, we observed that the H7N9 viruses isolated from humans had acquired critical mutations that made them more 'human-like'. It is therefore imperative to take strong measures to control the spread of H7N9 viruses in birds and humans to prevent further threats to human health. | SHI JianZhong DENG GuoHua LIU PeiHong ZHOU JinPing GUAN LiZheng LI WenHui LI XuYong GUO Jing WANG GuoJun FAN Jun WANG JinLiang LI YuanYuan JIANG YongPing LIU LiLing TIAN GuoBin LI ChengJun CHEN HuaLan | 2013 | Chinese Science Bulletin2013，58，16： | 85 |
| 2 | Prevalence of Nontraumatic Osteonecrosis of the Femoral Head and its Associated Risk Factors in the Chinese Population: Results from a Nationally Representative Survey显示文摘 | De-Wei Zhao Mang Yu Kai Hu Wei Wang Lei Yang Ben-Jie Wang Xiao-Hong Gao Yong-Ming Guo Yong-Qing Xu Yu-Shan Wei Si-Miao Tian Fan Yang Nan Wang Shi-Bo Huang Hui Xie Xiao-Wei Wei Hai-Shen Jiang Yu-Qiang Zang Jun Ai Yuan-Liang Chen Guang-Hua Lei Yu-Jin Li Geng Tia Zong-Sheng Li Yong Cao Li Ma | 2015 | Chinese Medical Journal2015，，21： | 151 |
| 3 | A complete sequence and comparative analysis of a SARS-associated virus(Isolate BJ01)显示文摘The genome sequence of the Severe Acute Respiratory Syndrome (SARS)-associated virus provides essential information for the identification of pathogen(s), exploration of etiology and evolution, interpretation of transmission and pathogenesis, development of diagnostics, prevention by future vaccination, and treatment by developing new drugs. We report the complete genome sequence and comparative analysis of an isolate (BJ01) of the coronavirus that has been recognized as a pathogen for SARS. The genome is 29725 nt in size and has 11 ORFs (Open Reading Frames). It is composed of a stable region encoding an RNA-dependent RNA polymerase (composed of 2 ORFs) and a variable region representing 4 CDSs (coding sequences) for viral structural genes (the S, E, M, N proteins) and 5 PUPs (putative uncharacterized proteins). Its gene order is identical to that of other known coronaviruses. The sequence alignment with all known RNA viruses places this virus as a member in the family of Coronaviridae. Thirty putative substitutions have been identified by comparative analysis of the 5 SARS- associated virus genome sequences in GenBank. Fifteen of them lead to possible amino acid changes (non-synonymous mutations) in the proteins. Three amino acid changes, with predicted alteration of physical and chemical features, have been detected in the S protein that is postulated to beinvolved in the immunoreactions between the virus and its host. Two amino acid changes have been detected in the Mprotein, which could be related to viral envelope formation. Phylogenetic analysis suggests the possibility of non-human origin of the SARS-associated viruses but provides noevidence that they are man-made. Further efforts should focus on identifying the etiology of the SARS-associated virus and ruling out conclusively the existence of otherpossible SARS-related pathogen(s). | QIN E'de ZHU Qingyu YU Man FAN Baochang CHANG Guohui SI Bingyin YANG Bao PENG Wenming JIANG Tao LIU Bohua DENG Yongqiang LIU Hong ZHANG Yu WANG Cui LI Yuquan GAN Yonghua LI Xiaoyu L Fushuang TAN Gang CAO Wuchun, YANG Ruifu Institute of Microbiology and Epidemiology, Chinese Academy of Military Medical Sciences, Beijing 100071, China WANG Jian, LI Wei, XU Zuyuan, LI Yan, WU Qingfa, LIN Wei, CHEN Weijun, TANG Lin, DENG Yajun, HAN Yujun, LI Changfeng, LEI Meng, LI Guoqing, LI Wenjie, L Hong, SHI Jianping, TONG Zongzhong, ZHANG Feng, LI Songgang, LIU Bin, LIU Siqi, DONG Wei, WANG Jun, Gane K-S Wong, YU Jun & YANG Huanming* Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300 National Center for Genome Information, Beijing 101300, China | 2003 | Chinese Science Bulletin2003，48，10： | 121 |
| 4 | Association of H.pylori infection with gastric carcinoma:a Meta analysis显示文摘AIM: To follow the principles of evidence based medicine to reach the integrated results of these studies.METHODS: Twenty-one papers of case-control studies were selected, including 11 on gastric cancer, 7 on precancerous lesion of stomach and 3 on lymphoma of stomach: Meta analysis was used to sum up the odds ratios (OR) of these studies.RESULTS: H. Pylori vsgastric cancer (intestinal and diffuse type): the odds ratio from the fixed effect model is 3.0016(95% Cl 2.4197-3.7234, P < 0.001 ). H. Pylori vs precancerous lesion of stomach: a random effect model was used to calculate the summary odds ratio and its value is 2.5635 (95% Cl: 1.8477-3.5566, P < 0.01). H. Pylori vs lymphoma of stomach: though the quantity of literature is too small to make Meta analysis, the data of these 3 studies show that lymphoma of stomach is highly associated with H. Pylori infections.CONCLUSION: Since it had been revealed that H. Pylori infection pre-exists in gastric carcinoma and precancerous lesions, the results of Meta analysis present a strong evidence to support the conclusion that H. Pylori infection is a risk factor for gastric carcinoma. | Fu-Bo Xue~1 Yong-Yong Xu~1 Yi Wan~1 Bo-Rong Pan~2 Jun Ren~2 Dai-Ming Fan~3 1 Department of Health Statistics,Department of2 Oncology3 Gastroenterology of XiJing Hospital,the Fourth Military Medical University,Xi’an 710032,Shaanxi Province,China | 2001 | World Journal of Gastroenterology2001，7，6： | 66 |
| 5 | Persistence and clearance of viral RNA in 2019 novel coronavirus disease rehabilitation patients显示文摘Background:A patient’s infectivity is determined by the presence of the virus in different body fluids,secretions,and excreta.The persistence and clearance of viral RNA from different specimens of patients with 2019 novel coronavirus disease(COVID-19)remain unclear.This study analyzed the clearance time and factors influencing 2019 novel coronavirus(2019-nCoV)RNA in different samples from patients with COVID-19,providing further evidence to improve the management of patients during convalescence.Methods:The clinical data and laboratory test results of convalescent patients with COVID-19 who were admitted to from January 20,2020 to February 10,2020 were collected retrospectively.The reverse transcription polymerase chain reaction(RT-PCR)results for patients’oropharyngeal swab,stool,urine,and serum samples were collected and analyzed.Convalescent patients refer to recovered non-febrile patients without respiratory symptoms who had two successive(minimum 24 h sampling interval)negative RT-PCR results for viral RNA from oropharyngeal swabs.The effects of cluster of differentiation 4(CD4)+T lymphocytes,inflammatory indicators,and glucocorticoid treatment on viral nucleic acid clearance were analyzed.Results:In the 292 confirmed cases,66 patients recovered after treatment and were included in our study.In total,28(42.4%)women and 38 men(57.6%)with a median age of 44.0(34.0-62.0)years were analyzed.After in-hospital treatment,patients’inflammatory indicators decreased with improved clinical condition.The median time from the onset of symptoms to first negative RT-PCR results for oropharyngeal swabs in convalescent patients was 9.5(6.0-11.0)days.By February 10,2020,11 convalescent patients(16.7%)still tested positive for viral RNA from stool specimens and the other 55 patients’stool specimens were negative for 2019-nCoV following a median duration of 11.0(9.0-16.0)days after symptom onset.Among these 55 patients,43 had a longer duration until stool specimens were negative for viral RNA than for throat swabs,with a median delay of 2.0(1.0-4.0)days.Results for only four(6.9%)urine samples were positive for viral nucleic acid out of 58 cases;viral RNA was still present in three patients’urine specimens after throat swabs were negative.Using a multiple linear regression model(F=2.669,P=0.044,and adjusted R2=0.122),the analysis showed that the CD4+T lymphocyte count may help predict the duration of viral RNA detection in patients’stools(t=-2.699,P=0.010).The duration of viral RNA detection from oropharyngeal swabs and fecal samples in the glucocorticoid treatment group was longer than that in the non-glucocorticoid treatment group(15 days vs.8.0 days,respectively;t=2.550,P=0.013)and the duration of viral RNA detection in fecal samples in the glucocorticoid treatment group was longer than that in the non-glucocorticoid treatment group(20 days vs.11 days,respectively;t=4.631,P<0.001).There was no statistically significant difference in inflammatory indicators between patients with positive fecal viral RNA test results and those with negative results(P>0.05).Conclusions:In brief,as the clearance of viral RNA in patients’stools was delayed compared to that in oropharyngeal swabs,it is important to identify viral RNA in feces during convalescence.Because of the delayed clearance of viral RNA in the glucocorticoid treatment group,glucocorticoids are not recommended in the treatment of COVID-19,especially for mild disease.The duration of RNA detection may relate to host cell immunity. | Yun Ling Shui-Bao Xu Yi-Xiao Lin Di Tian Zhao-Qin Zhu Fa-Hui Dai Fan Wu Zhi-Gang Song Wei Huang Jun Chen Bi-Jie Hu Sheng Wang En-Qiang Mao Lei Zhu Wen-Hong Zhang Hong-Zhou Lu | 2020 | Chinese Medical Journal2020，，9： | 58 |
| 6 | Cardioprotection of sevoflurane postconditioning by activating extracellular signal-regulated kinase 1/2 in isolated rat hearts^1显示文摘瞄准:1/2 免于的细胞外的调整信号的 kinase (英皇家空军之阶级最低之兵) 的激活是化学家灌注损害。ERK1/2 是否调停 sevoflurane 柱子调节的 cardioprotection 是未知的。我们测试了 sevoflurane 柱子调节是否经由 ERK1/2-depen-dent 机制生产 cardioprotection。方法:在协议 1 , 酒Langendorff 的 Sprague Dawley 老鼠心( n=84 , 12 每组)与假冒的组的异常,受到 90 min 灌注跟随的 30 min 局部缺血并且被分到未经治疗(控制)组,由 4 个周期列在后面是化学家帖子调节(各个的 25 s ),3%( v/v ) sevoflurane 帖子调节(为 5 min 和冲刷的 10 min ),并且 PD98059 溶剂 DMSO (<0.2%), ERK1/2 禁止者 PD98059 ( 20 μmol/L ),和 Sevo+PD 管理。在平衡的 30 min 的左室的 hemodynamics 和冠的流动分别地在灌注的 30, 60,和 90 min 被记录。尖锐梗塞尺寸被 triphenyltetrazolium 氯化物染色测量。线粒体的配置被一台电子显微镜观察。西方的污点分析被用来在灌注的结束决定 cytosolic 和 mitochondrial 细胞色素 c 的内容。在协议 2,在灌注的 15 min 以后,总数的表示和 ERK1/2 和它的下游的目标 p70S6K 的 phosphorylated 形式由西方的弄污是坚定的。结果:在基线 hemodynamics 的差别都没在试验性的组(P>0.05 ) 之中被观察。在灌注以后,与控制组相比, sevoflurane 柱子调节并且显著地是化学家柱子调节(P<0.05 ) 改进了功能的恢复并且(P<0.05 ) 大部分减少了心肌的梗塞尺寸(22.9%± 4 .6% 和 21.2%± 3 .8% ,对 39.4%± 5 .7% ,两 P<0.05 ) 。调停 Sevoflurane 的保护被 PD98059 废除。结论:由 sevoflurane 的麻药柱子调节有效地由在 vitro 激活 ERK1/2 免于灌注损坏。 | Hong-tao CHEN Cheng-xiang YANG Heng LI Cheng-jing ZHANG Xian-jie WEN Jun ZHOU You-ling Fan Teng HUANG Yin-ming ZENG | 2008 | Acta Pharmacologica Sinica2008，29，8： | 41 |
| 7 | 42,573 cases of hepatectomy in China: a multicenter retrospective investigation显示文摘Hepatectomy is currently routinely performed in most hospitals in China. China owns the largest population of liver diseases and the biggest number of liver resection cases. A nationwide multicenter retrospective investigation involving 112 hospitals was performed, and focused on liver resection for patients with hepatocellular carcinoma(HCC). 42,573 cases of hepatectomy were enrolled, and 18,275 valid cases of liver resection for HCC patients were selected for statistical analysis. The epidemiology of HCC, distribution of hepatectomy, postoperative complications and prognosis were finally analyzed. In the 18,275 HCC patients,81% had hepatitis B virus infection and 10% had hepatitis C virus infection. 38% of the HCC patients had normal Alphafetoprotein(AFP) level, and other 35% had an AFP level lower than 400 ng mL^(-1). In the study period, 97% of the hepatectomy for HCC were treated with open surgery, and 23.81% had vascular exclusion techniques. The operation time was(191.7±105.6) min,the blood loss was(546.0±562.8) m L, and blood transfusion was(543.0±1,035.2) m L. The median survival for HCC patients was 631 days, with 1-, 3-, and 5-year overall survival of 73.2%, 28.8% and 19.6%, respectively. Liver cirrhosis, multiple nodules,tumor thrombosis and high AFP level were risk factors that affect postoperative survival. | Binhao Zhang Bixiang Zhang Zhiwei Zhang Zhiyong Huang Yifa Chen Minshan Chen Ping Bie Baogang Peng Liqun Wu Zhiming Wang Bo Li Jia Fan Lunxiu Qin Ping Chen Jingfeng Liu Zhe Tang Jun Niu Xinmin Yin Deyu Li Songqing He Bin Jiang Yilei Mao Weiping Zhou Xiaoping Chen | 2018 | Science China(Life Sciences)2018，61，6： | 39 |
| 8 | Caspase-11/4 and gasdermin D-mediated pyroptosis contributes to podocyte injury in mouse diabetic nephropathy显示文摘Diabetic nephropathy(DN)is characterized by sterile inflammation with continuous injury and loss of renal inherent parenchyma cells.Podocyte is an essential early injury target in DN.The injury and loss of podocytes are closely associated with proteinuria,the early symptom of renal injury in DN.However,the exact mechanism for podocyte injury and death in DN remains ambiguous.In this study we investigated whether pyroptosis,a newly discovered cell death pathway was involved in DN.Diabetic mice were generated by high-fat diet/STZ injections.We showed that the expression levels of caspase-11 and cleavage of gasdermin D(GSDMD-N)in podocytes were significantly elevated,accompanied by reduced expression of podocyte makers nephrin and podocin,loss and fusion in podocyte foot processes,increased inflammatory cytokines NF-κB,IL-1β,and IL-18,macrophage infiltration,glomerular matrix expansion and increased urinary albumin to creatinine ratio(UACR).All these changes in diabetic mice were blunted by knockout of caspase-11 or GSDMD.Cultured human and mouse podocytes were treated with high glucose(30 mM),which significantly increased the expression levels of caspase-11 or caspase-4(the homolog of caspase-11 in human),GSDMD-N,NF-κB,IL-1β,and IL-18,and decreased the expression of nephrin and podocin.Either caspase-4 or GSDMD knockdown by siRNA significantly blunted these changes.In summary,our results demonstrate that caspase-11/4 and GSDMD-mediated pyroptosis is activated and involved in podocyte loss under hyperglycemia condition and the development of DN. | Qian Cheng Jing Pan Zhuan-li Zhou Fan Yin Hong-yan Xie Pan-pan Chen Jing-yao Li Pei-qing Zheng Li Zhou Wei Zhang Jun Liu Li-min Lu | 2021 | Acta Pharmacologica Sinica2021，42，6： | 33 |
| 9 | Wheat Grain Yield and Yield Stability in a Long-Term Fertilization Experiment on the Loess Plateau显示文摘To provide a scientific basis for sustainable land management, a 20-year fertility experiment was conducted in Changwu County, Shaanxi Province, China to investigate the effects of long-term application of chemical fertilizers on wheat grain yield and yield stability on the Loess Plateau using regression and stability analysis. The experiment consisted of 17 fertilizer treatments, containing the combinations of different N and P levels, with three replications arranged in a randomized complete block design. Nitrogen fertilizer was applied as urea, and P was applied as calcium superphosphate. Fertilizer rates had a large effect on the response of wheat yield to fertilization. Phosphorus, combined with N, increased yield significantly (P < 0.01). In the unfertilized control and the N or P sole application treatments, wheat yield had a declining trend although it was not statistically significant. Stability analysts combined with the trend analysis indicated that integrated use of fertilizer N and P was better than their sole application in increasing and sustaining the productivity of rainfed winter wheat. | HAO Ming-De FAN Jun WANG Quan-Jiu DANG Ting-Hui GUO Sheng-Li WANG Ji-Jun | 2007 | Pedosphere2007，17，2： | 28 |
| 10 | Directional hydraulic fracturing to control hard-roof rockburst in coal mines显示文摘Hard roof is the main factor that induces rock-burst.In view of the present obvious weakness of control measures for hard roof rockburst in domestic collieries,the mechanism and field application of directional hydraulic fracturing technology for rock-burst prevention have been investigated in this paper using theoretical analysis and numerical simulation.The results show that the weighting span of the main roof and the released kinetic energy as well as the total elastic energy decreased greatly after the directional fracturing of hard roof with the mining progression,thereby reducing the rockburst hazard degree to coal body.The directional hydraulic fracturing technology was carried out in 6305 working face of Jisan Coal Mine to prevent rockburst.Field practices have proved that this technology is much simpler and safer to operate with better prevention effect compared with blasting.By optimizing the operation procedures and developing a new technology of automated high-pressure delivery pipe,the maximum fracturing radius now reaches more than 9 m and the borehole depth exceeds 20 m.Additionally,drilling cutting method was applied to monitor the stress of the coal mass before and after the fracturing,and the drill cuttings dropped significantly which indicates that the burst prevention effect of directional hydraulic fracturing technology is very remarkable.The research results of this paper have laid a theoretical and practical foundation for the widespread application of the directional hydraulic fracturing technology in China. | Fan Jun Dou Linming He Hu Du Taotao Zhang Shibin Gui Bing Sun Xinglin | 2012 | International Journal of Mining Science and Technology2012，22，2： | 28 |
| 11 | Compound Danshen injection improves endotoxin-induced microcirculatory disturbance in rat mesentery显示文摘AIM: To investigate the effect of compound Danshen injection on lipopolysaccharide (LPS)-induced rat mesenteric microcirculatory dysfunctions and the underlying possible mechanism by an inverted intravital microscope and high-speed video camera system. METHODS: LPS was continuously infused through the jugular artery of male Wistar rats at the dose of 2 mg/kg per hour. Changes in mesenteric microcirculation,such as diameters of arterioles and venules,velocity of RBCs in venules,leukocyte rolling,adhesion and emigration,free radicals released from post-capillary venules,FITC- albumin leakage and mast cell degranulation,were observed through an inverted intravital microscope assisted with CCD camera and SIT camera. Meanwhile,the expression of adhesion molecules CD11b/CD18 and the production of free radical in neutrophils,and the expression of intercellular adhesion molecule 1 (ICAM-1) in human umbilical vein endothelial cells (HUVECs) were quantified by flow cytometry (FACS) in vitro. RESULTS: The continuous infusion with LPS resulted in a number of responses in microcirculation,including a significant increase in the positive region of venulestained with Monastral blue B,rolling and adhesion of leukocytes,production of oxygen radical in venular wall,albumin efflux and enhanced mast cell degranulation in vivo,all of which,except for the leukocyte rolling,were attenuated by the treatment with compound Danshen injection. Experiments performed in vitro further revealed that the expression of CD11b/CD18 and the production of oxygen free radical in neutrophils,and the expression of ICAM-1 in HUVECs were increased by exposure to LPS,and they were attenuated by compound Danshen injection. CONCLUSION: These results suggest that compound Danshen injection is an efficient drug with multi-targeting potential for improving the microcirculatory disturbance. | Jing-Yan Han Yoshinori Horie Soichiro Miura, Yasutada Akiba Jun Guo Dan Li Jing-Yu Fan Yu-Ying Liu Bai-He Hu Li-Hua An Xin Chang Man Xu De-An Guo Kai Sun Ji-Ying Yang Shu-Ping Fang Ming-Ji Xian Masahiro Kizaki Hiroshi Nagata Toshifumi Hibi | 2007 | World Journal of Gastroenterology2007，13，26： | 26 |
| 12 | Application of the SCS-CN Model to Runoff Estimation in a Small Watershed with High Spatial Heterogeneity显示文摘For reasons of simplicity, the most commonly used hydrological models are based on the Soil Conservation Service Curve Number (SCS-CN) model, which is probably a good choice for the estimation of runoff on the Loess Plateau of China; however, the high spatial heterogeneity, mainly caused by a fragmented landform and variations in soil type, may limit its applicability to this region. Therefore, applicability of the SCS-CN model to a small watershed, Liudaogou on the plateau, was evaluated and the most appropriate initial abstraction ratio (Ia/S) value in the model was quantified by the inverse method. The results showed that the standard SCS-CN model was applicable to the estimation of runoff in the Liudaogou watershed and the model performance was acceptable according to the values of relative error and Nash-Sutcliffe effciency. The most appropriate Ia/S value for the watershed was 0.22 because with this modified Ia/S value, the model performance was slightly improved. The model performance was not sensitive to the modification of the Ia/S value when one heavy rainfall event (50.1 mm) was not considered, which implied that the model, using a standard Ia/S value, can be recommended for the Liudaogou watershed because single rainfall events exceeding 50 mm seldom occurred in that region. The runoff amount predicted for the Liudaogou watershed by the SCS-CN model, using the modified Ia/S value, increased gradually with increasing rainfall when rainfall values were lower than 50 mm, whereas the predicted amount increased rapidly when the rainfall exceeded 50 mm. These findings may be helpful in solving the problem of serious soil and water loss on the Loess Plateau of China. | XIAO Bo WANG Qing-Hai FAN Jun HAN Feng-Peng DAI Quan-Hou | 2011 | Pedosphere2011，21，6： | 25 |
| 13 | Prevalence of Thyroid Nodules and Its Relationship with Iodine Status in Shanghai:a Population-based Study显示文摘Objective This study was designed to evaluate the prevalence of thyroid nodules(TNs) and its relationship with urine iodine concentrations(UICs) after the regional rapid economic growth and lifestyle changes. Methods A cross-sectional survey was conducted in the general population aged 15-69 years. A questionnaire regarding general and personal characteristics and relevant information was administered. Ultrasonography of the thyroid was performed, and serum triiodothyronine(T3), tetraiodothyronine(T4), serum thyroid stimulating hormone(TSH), free triiodothyronine(FT3), free tetraiodothyronine(FT4), thyroglobulin antibody(Tg Ab), thyroid peroxidase antibody(TPOAb), and TSH receptor antibody(TRAb) levels were measured for each individual subject. Results The prevalence rates of TNs in the whole population, females and males were 27.76%, 34.04%, and 21.60%, respectively. The prevalence of multiple nodules increased with age, whereas the prevalence peaks differed between males and females. The median UICs in the whole population and females with non-TNs were higher than those of subjects with TNs(P=0.0035, P=0.0068). The median UICs in subjects with a single TN were higher than those in subjects with multiple TNs(P=0.0164, P=0.0127). The result showed a U-shaped curve relationship between UIC and prevalence of TNs. The prevalence of TNs was the lowest when the UIC was 140-400 μg/L. Conclusion The prevalence of TNs was nearly 30% and increased with age. The relationship between UIC and prevalence of TNs is U-shaped, with an increase in risk when the UIC was <140 μg/L and >400 μg/L. Very low or high UIC levels need attention and correction. | SONG Jun ZOU Shu Rong GUO Chang Yi ZANG Jia Jie ZHU Zhen Ni MI Ming HUANG Cui Hua YU Hui Ting LU Xi RUAN Ye WU Fan | 2016 | Biomedical and Environmental Sciences2016，29，6： | 25 |
| 14 | Chinese Trauma Surgeon Association for management guidelines of vacuum sealing drainage application in abdominal surgeries-Update and systematic review显示文摘Vacuum sealing drainage (VSD) is frequently used in abdominal surgeries. However, relevant guidelines are rare. Chin ese Trauma Surge on Associati on orga nized a committee composed of 28 experts across China in July 2017, aiming to provide an evidence-based recommendation for the application of VSD in abdominal surgeries.Eleven questions regarding the use of VSD in abdominal surgeries were addressed:(1) which type of materials should be respectively chosen for the intraperitoneal cavity, retroperitoneal cavity and superficial incisions?(2) Can VSD be preventively used for a high-risk abdominal incision w让h primary suture?(3) Can VSD be used in severely contaminated/infected abdominal surgical sites?(4) Can VSD be used for temporary abdominal cavity closure under some special conditions such as severe abdominal trauma, infection, liver transplantation and intra-abdominal volume increment in abdominal compartment syndrome?(5) Can VSD be used in abdominal organ inflammation, injury, or postoperative drainage?(6) Can VSD be used in the treatment of intestinal fistula and pancreatic fistula?(7) Can VSD be used in the treatment of intra-abdominal and extra-peritoneal abscess?(8) Can VSD be used in the treatment of abdominal wall wounds, wound cavity, and defects?(9) Does VSD in crease the risk of bleeding?(10) Does VSD increase the risk of intestinal wail injury?(11) Does VSD increase the risk of peritoneal adhesion? Focusing on these questions, evidence-based recommendations were given accordingly. VSD was strongly recommended regarding the questions 2-4. Weak recommendations were made regarding questions 1 and 5-11. Proper use of VSD in abdominal surgeries can lower the risk of infection in abdominal incisions with primary suture, treat severely contaminated/infected surgical sites and facilitate temporary abdominal cavity closure. | Yang Li Pei-Yuan Li Shi-Jing Sun Yuan-Zhang Yao Zhan-Fei Li Tao Liu Fan Yang Lian-Yang Zhang Xiang-Jun Bai Jing-Shan Huo Wu-Bing He Jun Ouyang Lei Peng Ping Hu Yan-An Zhu Ping Jin Qi-Feng Shao Yan-Feng Wang Rui-Wu Dai Pei-Yang Hu Hai-Ming Chen Ge-Fei Wang Yong-Gao Wang Hong-Xu Jin Chang-Ju Zhu Qi-Yong Zhang Biao Shao Xi-Guang Sang Chang-Lin Yin | 2019 | Chinese Journal of Traumatology2019，22，1： | 26 |
| 15 | Nitrate Accumulation in Soil Profile of Dry Land Farming in Northwest China显示文摘A long-term fertilizer experiment on dry land of the Loess Plateau, northwest China, has been conducted since 1984 to study the distribution and accumulation of NO3-N down to a depth of 400 cm in the profile of a coarse-textured dark loessial soil after continuous winter wheat cropping. Thirteen fertilizer treatments consisted of four levels of N and P applied alone or in combination. Annual N and P (P2O5) rates were 0,45, 90, 135 and 180 kg ha-1. After 15 successive cropping cycles, the soil samples were taken from each treatment for analysis of NO3-N concentration. The results showed that NO3-N distribution in the soil profile was quite different among the treatments. The application of fertilizer N alone resulted in higher NO3-N concentration in the soil profile than the combined application of N and P, showing that application of P could greatly reduce the NO3-N accumulation. With an annual application of 180 kg N ha-1 alone, a peak in NO3-N accumulation occurred at 140 cm soil depth, and the maximum NO3-N concentration in the soils was 67.92 mg kg-1. The amount of NO3-N accumulated in the soil profile decreased as the cumulative N uptake by the winter wheat increased. Application of a large amount of N resulted in lower N recoveries in winter wheat and greater NO3-N accumulation in soil profile. NO3-N did not enter underground water in the study region; therefore, there is no danger of underground water pollution. Amount of NO3-N accumulation can be predicted by an equation according to annual N and P rates based on the results of this experiment. | FAN Jun, HAO Ming-De and SHAO Ming-AnState Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau, Institute of Soil and Water Conservation, the Chinese Academy of Sciences, Yangling 712100 (China). | 2003 | Pedosphere2003，13，4： | 25 |
| 16 | Expression of insulin-like growth factor Ⅱ and its receptor in hepatocellular carcinogenesis显示文摘INTRODUCTIONInsulin-like growth factor Ⅱ(IGF-Ⅱ) is a mitogenic peptide of 74 kD and is mostly synthesized in fetal liver tissue .IGF-Ⅱ is believed to play an important role in fetal growth and development and is involved in cellular proliferation and differentiation[1-5]. Recently ,several researchers have reported increased expression of the IGF-Ⅱgene in human hepatocellular carcinoma (HCC) and adjacent non-cancerous liver tissues [6-10]. | Zi Rong Fan Dong Hua Yang Jun Cui Han Rong Qin Chun Chi Huang Department of Gastroenterology, Zhujiang Hospital. The First Military Medical University, Guangzhou 510282.Guangdong Province. China | 2001 | World Journal of Gastroenterology2001，7，2： | 24 |
| 17 | Antisense telomerase RNA induced human gastric cancer cell apoptosis显示文摘INTRODUCTION Human tissue homeostasis is precisely regulated bycellular division,differentiation and death.Normalhuman somatic cells progressively lose telomererestriction fragment(TRF)length with eachsuccessive cell division,eventually leading tocellular quiescence,chromosomal end-degradationand apoptosis.On the contrary,stabilization oftelomere lengths by expressing telomerase,an RNA-dependent DNA polymerase,may be involved incellular immortality and carcinogenesis. | Fang Xin Zhang Xue Yong Zhang Dai Ming Fan Zi Yun Deng Yan Yan Han Ping Wu Jun Jie Fan | 2000 | World Journal of Gastroenterology2000，6，3： | 24 |
| 18 | The Hardy Rubber Tree Genome Provides Insights into the Evolution of Polyisoprene Biosynthesis显示文摘Eucommia ulmoides,也叫的强壮的橡胶树,是一棵经济地重要的树;然而,它的染色体顺序的缺乏 ?限制基本生物研究和这植物种的应用研究。这里,我们在场它的 1.2-Gb 染色体的一个高质量的集会(支架 N50 ?=? 1.88 Mb ) 与至少 26 ? 为 E 的 723 预言的基因。ulmoides,顺序 Garryales 的首先定序的染色体,它用联合定序的 Illumina 的综合策略被获得,定序的 PacBio,和印射的 BioNano。作为到 lamiids 和 campanulids 的姐妹 taxon, E。ulmoides 经历了三倍由核心 eudicots 而是没有进一步整个染色体的复制分享了的一个古老的染色体 ? 在最后 1.25 亿年里。E。ulmoides 为涉及压力回答和第二等的代谢物的生合成的多重基因展出高表达式层次或基因数字扩大,它可以说明它的可观的环境适应性。与橡胶树(Hevea brasiliensis ) 相对照,它生产 cis 聚异式戊二我烯, E。ulmoides 演变综合经由 farnesyl diphosphate synthases (FPS ) 的长链的 trans 聚异式戊二我烯。而且, FPS 和橡胶延伸 factor/small 橡胶粒子蛋白质基因家庭从 H 独立地被扩展。brasiliensis 系。这些结果提供新卓见进 E. 的生物学 ? ulmoides 和聚异式戊二我烯生合成的起源。 | Ta-na Wuyun Lin Wang Huimin Liu Xuewen Wang Liangsheng Zhang Jeffrey L. Bennetzen Tiezhu Li Lirong Yang Panfeng Liu Lanying Du Lu Wang Mengzhen Huang Jun Qing Iili Zhu Wenquan Bao Hongguo Li Qingxin Du Jingle Zhu Hong Yang Shuguang Yang Hui Liu Hui Yue Jiang Hu Suoliang Yu Yu Tian Fan Liang Jingjing Hu Depeng Wang Ruiwen Gao Dejun Li Hongyan Du | 2018 | Molecular Plant2018，11，3： | 24 |
| 19 | Establishment of an untransfected human corneal epithelial cell line and its biocompatibility with denuded amniotic membrane显示文摘AIM: To establish an untransfected human corneal epithelial (HCEP) cell line and characterize its biocompatibility with denuded amniotic membrane (dAM).METHODS: The torn HCEP pieces were primarily cultured in DMEM/F12 media (pH 7.2) supplemented with 20% fetal bovine serum and other necessary factors,yielding an HCEP cell line which was its growth performance,chromosome morphology,tumorigenicity and expression of marker proteins analyzed.In addition,the biocompatibility of HCEP cells with dAM was evaluated through histological and immunocytochemistry analyses and with light,electron and slit-lamp microscopies.RESULTS: HCEP cells proliferated to confluence in 3 weeks,which have been subcultured to passage 160.A continuous untransfected HCEP cell line,designated as utHCEPC01,was established with a population doubling time of 45.42 hours as was determined at passage 100.The cells retained HCEP cell properties as were approved by chromosomal morphology and the expression of keratin 3.They,with no tumorigenicity,formed a multilayer epithelium-like structure on dAMs through proliferation and differentiation during air-liquid interface culture,maintained expression of marker proteins including keratin 3 and integrin β1 and attached tightly to dAMs.The reconstructed HCEP was highly transparent and morphologically and structurally similar to the original.CONCLUSION: An untransfected and non-tumorigenic HCEP cell line was established in this study.The cells maintained expression of marker proteins.The cell line was biocompatible with dAM.It holds the potential of being used for in vitro reconstruction of tissue-engineered HCEP,promising for the treatment of diseases caused by corneal epithelial disorders. | Ting-Jun Fan Bin Xu Jun Zhao Hong-Shou Yang Rui-Xin Wang and Xiu-Zhong Hu | 2011 | International Journal of Ophthalmology(English edition)2011，4，3： | 22 |
| 20 | Tectonic dynamics and correlation of major earthquake sequences of the Xiaojiang and Qujiang-Shiping fault systems, Yunnan, China显示文摘The N-S trending Xiaojiang fault zone and the NW-SE trending Qujiang-Shiping fault zone are adjacent active fault systems and seismogenic zones associated with strong and major earthquakes in Yunnan, China. To understand the interaction of the two fault systems, and its probable influence on earthquake occurrences, this paper conducts a synthetic study based on data of active tectonics, historical earthquakes, relocated small earthquakes, GPS station velocities and focal mechanism resolutions. The study makes several conclusions. (1) The active southward motion of the western side of the Xiaojiang fault zone (i.e. the side of the Sichuan-Yunnan block) has a persistent and intensive effect on the Qujiang-Shiping fault zone. The later fault zone has absorbed and transformed the southward motion of the western side of the former fault zone through dextral strike-slip/shearing as well as transverse shortening/thrusting. (2) Along the Xiaojiang fault zone, the present sinistral strike-slip/shearing rate decreases from 10 and 8 mm/a on the northern, central and central-southern segments to 4 mm/a on the southern segment. The decreased rate has been adjusted in the area along and surrounding the Qujiang-Shiping fault zone through reverse-dextral faulting and distributed shearing and shortening. (3) The tectonic-dynamic relation between the Xiaojiang fault zone and the Qujiang-Shiping fault zone is also manifested by a close correlation of earthquake occurrences on the two fault zones. From 1500 to 1850 a sequence of strong and major earthquakes occurred along the Xiaojiang fault zone and its northern neighbor, the Zemuhe fault zone, which was characterized by gradually accelerating strain release, gradually shortening intervals between M≥7 events, and major releases occurring in the mid to later stages of the sequence. As a response to this sequence, after an 88-year delay, another sequence of 383 years (from 1588 to 1970) of strong and major earthquakes occurred on the Qujiang-Shiping fault zone, and had the same features in accelerating strain release and its temporal course. (4) Since there has been no M≥7 event for 177 years on the Xiaojiang fault zone, the potential risk of a strong or major earthquake occurring on this fault zone in the future should be noticed and studied further. | WEN XueZe DU Fang LONG Feng FAN Jun ZHU Hang | 2011 | Science China Earth Sciences2011，54，10： | 22 |
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