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| 1 | Neutrophil extracellular trap cell death requires both autophagy and superoxide generation显示文摘嗜中性的细胞外的陷井(网) 是能套住并且降级的细胞外的染色质结构微生物。他们从激活称为网络房间死亡,或 NETosis 的一个房间死亡程序的 neutrophils 产生。NETosis 的激活被显示了包含 NADPH oxidase 活动,原子信封的崩溃和大多数小粒膜,网的原子染色质和形成的 decondensation。我们报导在 phorbol,十四酸盐醋酸盐(PMA ) 刺激了 neutrophils,细胞内部的染色质 decondensation 和网形成跟随 autophagy 和 superoxide 生产,哪个被要求调停导致 PMA 的 NETosis 并且独立于对方发生。从有长期的 granulomatous 疾病的病人的 Neutrophils,缺乏 NADPH oxidase 活动,仍然展出导致 PMA 的 autophagy。相反地,导致 PMA 的 NADPH oxidase 活动没被 autophagy 的药理学抑制影响。有趣地,任何一个 autophagy 的抑制或 NADPH oxidase 阻止细胞内部的染色质 decondensation,它为 NETosis 和网形成是必要的,并且导致 apoptosis 的特点描绘的房间死亡。当 autophagy 或 NADPH oxidase 活动被阻止时,这些结果显示 apoptosis 可能为 NETosis 作为一个备份节目工作。 | Quinten Remij sen Tom Vanden Berghe Ellen Wirawan Bob Asselbergh Eef Parthoens Riet De Rycke Sam Noppen Michel Delforge Jean Wlllems Peter Vandenabeele | 2011 | Cell Research2011，21，2： | 46 |
| 2 | Modulating the microbiota in inflammatory bowel diseases: prebiotics, probiotics or faecal transplantation?显示文摘 | Kristin A. Verbeke Leen Boesmans Eef Boets | 2014 | Proceedings of the Nutrition Society2014，，4： | 2 |
| 3 | Outcam of an-terior cervicaldiscectomy and fusion as perceived by patientstreated for dominant axialmechanical cervical spine pain显示文摘 | Gervey TA Transfeldt EEf Malcolm JR | 2002 | Spine2002，27，16： | 1 |
| 4 | Phytoestrogens and cognitive function: a review显示文摘 | Mira Soni Tri Budi W. Rahardjo Rodiyah Soekardi Yenny Sulistyowati Lestariningsih Amina Yesufu-Udechuku Atik Irsan Eef Hogervorst | 2013 | Maturitas2013，，： | 1 |
| 5 | Increased serum lactate dehydrogenase isoenzyme 1 and macro creatine kinase type 2 in a patient with lung cancer显示文摘 | Eef G Egbert T | 1987 | Clin Chim Acta1987，168，1： | 1 |
| 6 | Matthews, Carol Brayne, Thyroid function and cognitive decline in the MRC Cognitive Function and Ageing Study 显示文摘 | Eef Hogervorst Felicia Huppert Fiona E | 2008 | Psychoneuroendocrinology2008，33，： | 1 |
| 7 | Double Standards in Quantitative Proteomics: Direct Comparative Assessment of Difference in Gel Electrophoresis and Metabolic Stable Isotope Labeling显示文摘 | Annemieke Kolkman Eef Dirksen HC Moniqu-e Slijper | 2005 | Mol Cell Proteomics2005，4，： | 1 |
| 8 | Cancer chemotherapy-in-duced diarrhoea and constipation:mechanisms of damage andprevention strategies显示文摘 | G ibs on R J K eefe DM | 2006 | Support Care Cancer2006，14，9： | 1 |
| 9 | Randomized trial of radiofrequency lunber facet denerva-tion for chronic low back pain显示文摘 | Van Kl eef M | 1999 | Spine1999，24，： | 1 |
| 10 | Decreased nocturnal growth hormone secretion and sleep fragmentation in combat-related posttraumatic stress disorder: potential predictors of impaired memory consolidation 显示文摘 | Saskia van Liempt Eric Vermetten Eef Lentjes | 2011 | Psychoneuroendocrinology2011，36，9： | 1 |
| 11 | The combination of reducing agents/iron as environmentally friendly alternatives for Co-based driers in the drying of alkyd paints 显示文摘 | FABRIZIO MICCICH'E EEF OOSTVEEN JACCO VAN HAVEREN | 2005 | Progress in Organic Coatings2005，53，： | 1 |
| 12 | Repeated photoporation with graphene quantum dots enables homogeneous labeling of live cells with extrinsic markers for fluorescence microscopy显示文摘In the replacement of genetic probes,there is increasing interest in labeling living cells with high-quality extrinsic labels,which avoid over-expression artifacts and are available in a wide spectral range.This calls for a broadly applicable technology that can deliver such labels unambiguously to the cytosol of living cells.Here,we demonstrate that nanoparticle-sensitized photoporation can be used to this end as an emerging intracellular delivery technique.We replace the traditionally used gold nanoparticles with graphene nanoparticles as photothermal sensitizers to permeabilize the cell membrane upon laser irradiation.We demonstrate that the enhanced thermal stability of graphene quantum dots allows the formation of multiple vapor nanobubbles upon irradiation with short laser pulses,allowing the delivery of a variety of extrinsic cell labels efficiently and homogeneously into live cells.We demonstrate high-quality time-lapse imaging with confocal,total internal reflection fluorescence(TIRF),and Airyscan superresolution microscopy.As the entire procedure is readily compatible with fluorescence(super resolution)microscopy,photoporation with graphene quantum dots has the potential to become the long-awaited generic platform for controlled intracellular delivery of fluorescent labels for live-cell imaging. | Jing Liu Ranhua Xiong Toon Brans Saskia Lippens Eef Parthoens Francesca Cella Zanacchi Raffaella Magrassi Santosh K.Singh Sreekumar Kurungot Sabine Szunerits Hannelore Bové Marcel Ameloot Juan C.Fraire Eline Teirlinck Sangram Keshari Samal Riet De Rycke Gaëlle Houthaeve Stefaan C.De Smedt Rabah Boukherroub Kevin Braeckmans | 2018 | Light(Science & Applications)2018，7，1： | 1 |
| 13 | Surface modification of cotton yarn with a DC glow discharge in ambient air 显示文摘 | TEMMERMAN T Eef LEYS Christophe | 2005 | Surface & Coatings Technology2005，，200： | 1 |
| 14 | Coded aperture imaging with uniformly redundant arrays 显示文摘 | enirnore EEF | 1978 | Applied Optics1978，17，3： | 1 |
| 15 | Bladder preservation in the treatment of muscle-invasive bladder cancer(MIBC):a review of the literature and a practical approach to therapy显示文摘 | Smith Z L Christodouleas J P K Eefe S M | 2013 | BJU Int2013，112，1： | 1 |
| 16 | Uniformly redundant arrays : digital reconstruction methods显示文摘 | enimor EEFe | 1981 | Applied Optics1981，20，10： | 1 |
| 17 | Mycorrhizal Hyphal Turnover as a Dominant Process for Carbon Input into Soil Organic Matter显示文摘 | Douglas L. Godbold Marcel R. Hoosbeek Martin Lukac M. Francesca Cotrufo Ivan A. Janssens Reinhart Ceulemans Andrea Polle Eef J. Velthorst Giuseppe Scarascia-Mugnozza Paolo Angelis Franco Miglietta Alessandro Peressotti | 2006 | Plant and Soil (-)2006，，1： | 1 |
| 18 | Protein synthesis persists during necrotic cell death 显示文摘 | Saelens X Nele F Eef P | 2005 | J Cell Biol2005，168，： | 1 |
| 19 | Evidence for further charmonium vector resonances显示文摘We discuss the shape of threshold signals in production cross sections of the reaction e+e- → D* Dˉ*, at the opening of the Ds* Dˉs* and Λc+ Λ+c channels. Furthermore, evidence for the ψ(3D), ψ(5S), ψ(4D), ψ(6S), ψ(5D), ψ(7S), ψ(6D), and ψ(8S) new charmonium vector resonances is presented, on the basis of data recently published by the BABAR Collaboration. Central masses and resonance widths are estimated. Confirmation of these resonances would be a huge step in lifting the precision level of hadron spectroscopy towards that of atomic spectroscopy, with far-reaching consequences for theory. | Eef van Beveren George Rupp | 2011 | Chinese Physics C2011，35，4： | 0 |
| 20 | Long-term live-cell microscopy with labeled nanobodies delivered by laser-induced photoporation显示文摘Fluorescence microscopy is the method of choice for studying intracellular dynamics.However,its success depends on the.availability of specific and stable markers.A prominent example of markers that are rapidly gaining interest are nanobodies(Nbs.-15 kDa),which can be functionalized with bright and photostable organic fluorophores.Due to their relatively small size and high specificity,Nbs offer great potential for high-quality long-term subcellular imaging,but suffer from the fact that they cannot spontaneously cross the plasma membrane of live cells.We have recently discovered that laser-induced photoporation is well suited to deliver extrinsic labels to living cells without compromising their viability.Being a laser-based technology,it is readily compatible with light microscopy and the typical cell recipients used for that.Spurred by these promising initial results,we demonstrate here for the first time successful long-term imaging of specific subcellular structures with labeled nanobodies in living cells.We illustrate this using Nbs that target GFP/YFP-protein constructs accessible in the cytoplasm,actin-bundling protein Fascin,and the histone H2A/H2B heterodimers.With an efficiency of more than 80%labeled cells and minimal toxicity(-2%),photoporation proved to be an excellent intracellular delivery method for Nbs.Time-lapse microscopy revealed that cell division rate and migration remained unaffected,confirming excellent cell viability and functionality.We conclude that laser-induced photoporation labeled Nbs can be easily delivered into living cells,laying the foundation for further development of a broad range of Nbs with intracellular targets as a toolbox for long-term live-cell microscopy. | Jing Liu Tim Hebbrecht Toon Brans Eef Parthoens Saskia Lippens Chengnan Li Herlinde De Keersmaecker Winnok H.De Vos Stefaan C.De Smedt Rabah Boukherroub Jan Gettemans Ranhua Xiong Kevin Braeckmans | 2020 | Nano Research2020，13，2： | 0 |
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