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| 1 | Characterization of two rat models of cystic fibrosis—KO and F508del CFTR—Generated by Crispr-Cas9显示文摘Background: Genetically engineered animals are essential for gaining a proper understanding of the disease mechanisms of cystic fibrosis(CF). The rat is a relevant laboratory model for CF because of its zootechnical capacity, size, and airway characteristics, including the presence of submucosal glands.Methods: We describe the generation of a CF rat model(F508 del) homozygous for the p.Phe508 del mutation in the transmembrane conductance regulator(Cftr) gene. This model was compared to new Cftr-/-rats(CFTR KO). Target organs in CF were examined by histological staining of tissue sections and tooth enamel was quantified by micro-computed tomography. The activity of CFTR was evaluated by nasal potential difference(NPD) and short-circuit current measurements. The effect of VX-809 and VX-770 was analyzed on nasal epithelial primary cell cultures from F508 del rats.Results: Both newborn F508 del and Knock out(KO) animals developed intestinal obstruction that could be partly compensated by special diet combined with an osmotic laxative. The two rat models exhibited CF phenotypic anomalies such as vas deferens agenesis and tooth enamel defects. Histology of the intestine, pancreas, liver, and lungs was normal. Absence of CFTR function in KO rats was confirmed ex vivo by short-circuit current measurements on colon mucosae and in vivo by NPD, whereas residual CFTR activity was observed in F508 del rats. Exposure of F508 del CFTR nasal primary cultures to a combination of VX-809 and VX-770 improved CFTR-mediated Cl-transport.Conclusions: The F508 del rats reproduce the phenotypes observed in CFTR KO animals and represent a novel resource to advance the development of CF therapeutics. | Elise Dreano Marc Bacchetta Juliette Simonin Louise Galmiche Claire Usal Lotfi Slimani Jérémy Sadoine Laurent Tesson Ignacio Anegon Jean-Paul Concordet Aurélie Hatton Lucile Vignaud Danielle Tondelier Isabelle Sermet-Gaudelus Marc Chanson Charles-Henry Cottart | 2019 | Animal Models and Experimental Medicine2019，2，4： | 3 |
| 2 | IL-6 Promotes compensatory liver regeneration in cirrhotic rat after partial hepatectomy显示文摘 | Guido A.M. Tiberio Laura Tiberio Anna Benetti Edoardo Cervi Nadia Montani Michel Dreano Gianni Garotta Katia Cerea Nathalie Steimberg GianPietro Pandolfo Andrea Ferrari-Bravo Giovanna Mazzoleni Stefano M. Giulini Luisa Schiaffonati | 2008 | Cytokine2008，，3： | 1 |
| 3 | NOD2/CARD15 gene polymorphisms in Crohn’s disease: a genotype–phenotype analysis显示文摘 | Denis Heresbach Véronique Gicquel-Douabin Brigitte Birebent Pierre-Nicolas D’halluin Nathalie Heresbach-Le Berre Stéphane Dreano Laurent Siproudhis Alain Dabadie Michel Gosselin Jean Mosser Gilbert Semana Jean-Fran?ois Bretagne Jacqueline Yaouanq | 2004 | European Journal of Gastroenterology & Hepatology2004，，1： | 1 |
| 4 | Inhibition of CYP3A,CYPIA and CYP2EI activities by resveratrol and other non volatile red wine components显示文摘 | PIVER B BERTHOU F DREANO Y | 2001 | Toxicol Lett2001，125，13： | 1 |
| 5 | Inhibition of CYP3A,CYP1A and CYP2E1 activities by resveratrol and other non volatile r ed wine com ponents 显示文摘 | Piver B Berthou F Dreano Y | 2001 | Toxicol Lett2001，125，13： | 1 |
| 6 | 环氧花生四烯酸、功能受损的一氧化氮和内皮素1途径变化可造成原发性高血压患者传导性动脉内皮功能紊乱显示文摘研究人员试图运用功能和生物学方法确定环氧花生四烯酸、一氧化氮/活性氧平衡及内皮素1在原发性高血压患者传导性动脉内皮功能紊乱中所起的作用。方法与结果:研究对象为未经治疗的原发性高血压患者28例和正常血压者30名,测定其由于手部皮肤加热引起内皮依赖性血流介导的血管扩张过程中桡动脉的直径和平均壁剪切力。 | Bellien J Iacob M Remy-Jouet I Lucas D Monteil C Guti- errez L Vendeville C Dreano Y Mercier A Thuillez C Joannides R 刘莉 叶鹏 | 2012 | 中华高血压杂志2012，20，5： | 1 |
| 7 | Identification of thecytochrome P45o ID a family as the enzymes involved in then-demethylation of tamoxifen in human liver micro- somes显示文摘 | Jacolot F Simon I Dreano I | 1991 | Biochem Pharmacol1991，41，12： | 1 |
| 8 | Differential inhibition of human cytochrome P450 enzymes by epsilon-viniferin, the dimer of resveratrol: comparison with resveratrol and polyphenols from alcoholized beverages显示文摘 | PIVER B BERTHOU F DREANO Y | 2003 | Life Sci2003，3，9： | 1 |
| 9 | Determination of epoxyeicosatrienoic acids in human red blood cells and plasma by GC/MS in the NICI mode显示文摘 | Goulitquer S Dreano Y Berthou F | 2008 | J Chromatogr B2008，876，： | 1 |
| 10 | Recombinant follicle stimulating hormone: development of the first biotechnology product for the treatment of infertility 显示文摘 | Loumaye E Dreano M Galazka | 1998 | Hum Reprod Update1998，4，： | 1 |
| 11 | High performance liquid chromatographic analysis of tamoxifen,toremifene and their major human metabolites显示文摘 | Berthou F Dreano Y | 1993 | J Chromatogr1993，616，： | 1 |
| 12 | Inhibition of CYP3A, CYP1A and CYP2E1 activities by resveratrol and other non volatile red wine components 显示文摘 | Bertrand Piver Franc ois Berthou Yvonne Dreano | 2001 | Toxicol Lett2001，125，13： | 1 |
| 13 | Lymphocyte activation gene-3 induces tumor regression and antitumor immune responses显示文摘 | Prigent P Eimir S Dreano M | 1999 | Eur J Immunol1999，29，12： | 1 |
| 14 | Differential inhibi- tion of human cytochrome P450 enzymes by 6 - viniferin, the dimer of resveratrol: comparison with resveratrol and polyphenols from alcoholized beverages 显示文摘 | Piver B Berthou F Dreano Y | 2003 | Life Sci- ences2003，73，9： | 1 |
| 15 | Prevention of neuron and oligodendrocyte degeneration by interleukin-6 (IL-6) and IL-6 receptor/IL-6 fusion protein in organotypic hippocampal slices显示文摘 | Marina Pizzi Ilenia Sarnico Flora Boroni Marina Benarese Michel Dreano Gianni Garotta Alessandra Valerio PierFranco Spano | 2003 | Molecular and Cellular Neuroscience2003，，2： | 1 |
| 16 | Human cytomegalovirus stimulates cellular IKK2 activity and requires the enzyme for productive replication显示文摘 | Caposio P Dreano M Garotta G | | 0，，06： | 1 |
| 17 | Differential inhibition of human cytochrome P450 enzymes by epsilon-viniferin,the dimmer of resveratrol:comparision with resveratrol and polyphenols from alcoholized beverages显示文摘 | Piver B Berthou F Dreano Y | 2003 | Life Science2003，73，9： | 1 |
| 18 | Effects of Cycling Alone or in a Sheltered Position on Subsequent Running Performance during a Triathlon显示文摘 | HAUSSWIRTH C LEHENAFF D DREANO P | 1999 | Med Sci Sports Exe1999，31，4： | 1 |
| 19 | Inhibition of CYP3A, CYP1A and CYP2E1 activities by resvemtrol and other non volatile red wine components显示文摘 | Piver B Berthou F Dreano Y | 2001 | Toxicol Lett2001，125，13： | 1 |
| 20 | Inhibition of CYP3A, CYP1A and CYP2E1 activities by resveratrol and other non volatile red wine components 显示文摘 | PIVER B BERTHOU F DREANO Y | 2001 | Toxicol Lett2001，125，13： | 1 |
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