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| 1 | Hepatitis C virus infection down-regulates the expression of peroxisome proliferator-activated receptor a and carnitine palmitoyl acyl-CoA transferase 1A显示文摘AIM: To elucidate the role of the peroxisome proliferator-activated receptor α (PPARα) and its target gene carnitine palmitoyl acyl-CoA transferase 1A (CPT1A)in the pathogenesis of hepatitis C virus (HCV) infection.METHODS: Liver samples were collected from the patients with chronic HCV infection and controls. HepG2cells were transfected with vector pEF352neo carrying.Two independent clones (clone N3 and N4) stably expressing HCV core protein were analyzed. Total RNA was extracted from cells and liver tissues. PPARα and CPT1A mRNAs were quantified by real-time polymerase chain reaction (PCR) using SYBR Green Master. Total extracted proteins were separated by polyacrylamide gel electrophoresis, and electroblotted. Membranes were incubated with the anti-PPARα antibody, then with a swine anti-rabbit IgG conjugated to horseradish peroxidase for PPARα. Protein bands were revealed by an enhanced chemiluminescence reaction for PPARα. For immunohistochemical staining of PPARα, sections were incubated with the primary goat polyclonal antibody directed against PPARα at room temperature.RESULTS: Real-time PCR indicated that the PPARα level and expression level of CPT1A gene in hepatitis C patients lowered significantly as compared with the controls (1.8±2.8 vs 13±3.4, P = 0.0002; 1.1±1.5 vs 7.4+1, ,P = 0.004). Western blot results showed that the level of PPARα protein in the livers of hepatitis C patients was lower than that in controls (2.3±0.3 vs 3.6±0.2,P = 0.009). The immunohistochemical staining results in chronic hepatitis C patients indicated a decrease in PPARα staining in hepatocytes compared with those in the control livers. The in vitro studies found that in the N3 and N4 colon stably expressing HCV core protein, the PPARα mRNA levels were significantly lower than that in the controls.CONCLUSION: The impaired intrahepatic PPARα expression is associated with the pathogenic mechanism in hepatic injury during chronic HCV infection. HCV infection reduced the expression of PPARα and CPT1A at the level of not only mRNAs but also proteins. PPARα plays an important role in the pathogenesis of chronic HCV infection, but the impaired function of this nuclear receptor in HCV infection needs further studies. | Yang Cheng Sébastien Dharancy Mathilde Malapel Pierre Desreumaux | 2005 | World Journal of Gastroenterology2005，11，48： | 11 |
| 2 | Role of immunosuppression and tumor differentiation in predicting recurrence after liver transplantation for hepatocellular carcinoma: A multicenter study of 412 patients显示文摘AIM: To assess pre-orthotopic liver transplantation (OLT) factors that could be evaluated pre-operatively or con- trolled post-operatively associated with hepatocellular carcinoma (HCC) recurrence and disease-free survival after liver transplantation (LT).METHODS: Four hundred and twelve patients trans- planted for HCC between 1988 and 1998 in 14 French centers, who survived the postoperative period were studied. Kaplan Meier estimates were calculated for 24 variables potentially associated with recurrence of HCC. Uni- and multivariate analyses were conducted to iden- tify independent predictors of recurrence. RESULTS: Overall 5-year disease-free survival was 57.1%. By univariate analysis, variables associated with disease-free survival were: presence of cirrhosis (P = 0.001), etiology of liver disease (P = 0.03), α fetoprotein level (< 200, 200 to 2000, or > 2000; P < 0.0001), γ-GT activity (N, N to 2N or > 2N; P = 0.02), the number of nodules (1, 2-3 or ≥ 4; P = 0.02), maximal diameter of the largest nodule (< 3 cm, 3 to 5 cm or > 5 cm; P < 0.0001), the sum of the diameter of the nodules (< 3 cm, 3 to 5 cm, 5 to 10 cm or >10 cm; P < 0.0001), bi- lobar location (P = 0.01), preoperative portal thrombosis (P < 0.0001), peri-operative treatment of the tumor (P = 0.002) and chemoembolization (P = 0.03), tumor differentiation (P = 0.01), initial type of calcineurin inhibitor (P = 0.003), the use of antilymphocyte antibodies (P = 0.02), rejection episodes (P = 0.003) and period of LT (P < 0.0001). By multivariate analysis, 6 variables were independently associated with HCC recurrence: maximal diameter of the largest nodule (P < 0.0001), time of LT (P < 0.0001), tumor differentiation (P < 0.0001), use of anti-lymphocyte antibody (ATG) or anti-CD3 antibody (OKT3) (P = 0.005), preoperative portal thrombosis (P = 0.06) and the number of nodules (P = 0.06). CONCLUSION: This study identif ies immunosuppression, through the use of ATG or OKT3, as a predictive factor of tumor recurrence, and confi rms the prognostic value of tumor differentiation. | Thomas Decaens Franoise Roudot-Thoraval Solange Bresson-Hadni Carole Meyer Jean Gugenheim Francois Durand Pierre-Henri Bernard Olivier Boillot Philippe Compagnon Yvon Calmus Jean Hardwigsen Christian Ducerf Georges Philippe Pageaux Sébastien Dharancy Olivier Chazouillères Daniel Cherqui Christophe Duvoux | 2006 | World Journal of Gastroenterology2006，12，45： | 10 |
| 3 | Early switch to pentoxifylline in patients with severe alcoholic hepatitis is inefficient in non-responders to corticosteroids显示文摘 | Alexandre Louvet Emmanuel Diaz Sébastien Dharancy Hugues Coevoet Frédéric Texier Thierry Thévenot Pierre Deltenre Valérie Canva Christophe Plane Philippe Mathurin | 2007 | Journal of Hepatology2007，，3： | 2 |
| 4 | Transjugular intrahepatic portosystemic shunt in refractory ascites: a meta-analysis 显示文摘 | Dehenre P Mathurin P Dharancy S | 2005 | Liver International2005，25，2： | 1 |
| 5 | Meta-analysis:evaluation of adjuvant therapy after curative liver resection for hepatocellular carcinoma显示文摘 | Raynant B Dharancy S | 2003 | Aliment Pharmseol Ther2003，17，10： | 1 |
| 6 | Meta-analysis:evaluation of adjuvant therapy after curative liver resection for hepatocellular carcinoma显示文摘 | Mathurin P Raynard B Dharancy S | 2003 | Aliment Pharmacol Ther2003，17，： | 1 |
| 7 | Prospective Study of the Long-Term Effects of Bariatric Surgery on Liver Injury in Patients Without Advanced Disease显示文摘 | Philippe Mathurin Antoine Hollebecque Laurent Arnalsteen David Buob Emmanuelle Leteurtre Robert Caiazzo Marie Pigeyre Hélène Verkindt Sébastien Dharancy Alexandre Louvet Monique Romon Fran?ois Pattou | 2009 | Gastroenterology2009，，2： | 1 |
| 8 | Interleukin-15 production during liver allograft rejection in humans 显示文摘 | Conti F Frappier J Dharancy S | 2003 | Transplantation2003，76，1： | 1 |
| 9 | Interleukin-15 production during liver allograft rejection in humans.显示文摘 | Conti F Frappier J Dharancy S | | 0，，： | 1 |
| 10 | Early switch to pentoxifylline in patients with severe alcoholic hepatitis is inefficient in non-responders to corticosteroids显示文摘 | Alexandre Louvet Emmanuel Diaz Sébastien Dharancy Hugues Coevoet Frédéric Texier Thierry Thévenot Pierre Deltenre Valérie Canva Christophe Plane Philippe Mathurin | 2007 | Journal of Hepatology2007，，3： | 1 |
| 11 | Impaired expression of the peroxisome proliferator–activated receptor alpha during hepatitis C virus infection显示文摘 | Sébastien Dharancy Mathilde Malapel Gabriel Perlemuter Tania Roskams Yang Cheng Laurent Dubuquoy Philippe Podevin Filoména Conti Valérie Canva David Philippe Luc Gambiez Philippe Mathurin Jean-Claude Paris Kristina Schoonjans Yvon Calmus Stanislas Pol Joh | 2005 | Gastroenterology2005，，2： | 1 |
| 12 | The Evolution of Severe Steatosis After Bariatric Surgery Is Related to Insulin Resistance显示文摘 | Philippe Mathurin Florent Gonzalez Olivier Kerdraon Emmanuelle Leteurtre Laurent Arnalsteen Antoine Hollebecque Alexandre Louvet Sébastien Dharancy Perrine Cocq Thomas Jany Jeanne Boitard Pierre Deltenre Monique Romon Fran?ois Pattou | 2006 | Gastroenterology2006，，6： | 1 |
| 13 | Liver Transplantation for Hepatocellular Carcinoma: A Model Including α-Fetoprotein Improves the Performance of Milan Criteria显示文摘 | Christophe Duvoux Fran?oise Roudot–Thoraval Thomas Decaens Fabienne Pessione Hanaa Badran Tullio Piardi Claire Francoz Philippe Compagnon Claire Vanlemmens Jérome Dumortier Sébastien Dharancy Jean Gugenheim Pierre–Henri Bernard René Adam Sylvie Radenne Fa | 2012 | Gastroenterology2012，，4： | 1 |
| 14 | A double-blind randomized controlled trial of infliximab associated with prednisolone in acute alcoholic hepatitis显示文摘 | Naveau S Martin SC Dharancy S | 2004 | Hepatology2004，39，5： | 1 |
| 15 | Mem-analysis:ealuation of adjuant therapy after curatie lie resection for hepatocellular carcinoma显示文摘 | Mathurin P Raynard B Dharancy S | 2003 | Aliment Pharmacol Ther2003，17，10： | 1 |
| 16 | Impact of emerging hepatitis C virus treatments on future needs for liver transplantation in France: A modelling approach显示文摘 | Sylvie Deuffic-Burban Philippe Mathurin Isabelle Rosa Anne-Marie Bouvier Amélie Cannesson Abbas Mourad Valérie Canva Alexandre Louvet Pierre Deltenre Emmanuel Boleslawski Stéphanie Truant Fran?ois-René Pruvot Sébastien Dharancy | 2014 | Digestive and Liver Disease2014，，2： | 1 |
| 17 | Role of peroxisome proliferator-activated receptor gamma and retinoid X receplor het-erodimer in hepatogastroentemlogicl diseases显示文摘 | Dubuquy L Dharancy S Nutter S | 2002 | Lancet2002，360，： | 1 |
| 18 | Interleukin-15produefion during liver allograft rejection in humans 显示文摘 | Conti F Frappier J Dharancy S | 2003 | Transplantation2003，76，1： | 1 |
| 19 | Transjugular intrahepat, ic portosystemie shunt in refractory aseites: a meta-analysis显示文摘 | Dehenre P Mathurin P Dharancy S | 2005 | Liver Int2005，25，2： | 1 |
| 20 | Transjugular intrahepatic portosystemic shunt in refractory ascites : a meta-analysis 显示文摘 | Deltenre P Mathurin P Dharancy S | 2005 | Liver Int2005，25，： | 1 |
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