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| 1 | 以β-淀粉样蛋白为靶标的藏药对阿尔采末病Tg2576转基因鼠学习记忆的影响显示文摘目的探讨藏药七十味珍珠丸(RNSP)以β-淀粉样蛋白为靶标对阿尔采末病(AD)动物模型学习记忆作用的机制。方法25只13~14mon♀Tg2576转基因鼠和同龄♀C57BL/6XDBA鼠为正常对照组给予七十味珍珠丸灌胃8wk。利用Y-迷宫明暗分辨学习和旷场实验观察转基因鼠学习、记忆和行为学变化。蛋白免疫印迹法测定鼠血清β淀粉样蛋白(Aβ)和β位点APP内切酶(BACE-1)含量,免疫组织化学法观察Aβ在脑的表达。结果通过Y-迷宫测试,藏药Tg2576治疗组达标所需要的训练时间明显低于Tg2576对照组(P<0.01)。旷场实验测定发现Tg2576治疗组在中央格停留时间明显减低,跨格和站立次数增加,粪便颗粒数也明显减少。蛋白印迹法定量分析,经RNSP治疗的转基因鼠血清Aβ和BACE-1含量较对照组明显减低。RNSP能够明显减少大脑皮层和海马周围老年淀粉样斑块的数目和面积。结论藏药七十味珍珠丸改善Tg2576转基因鼠学习和空间记忆以及探索运动能力,减少焦虑状态等认知行为可能通过抑制BACE-1(即β-分泌酶)的表达来减少老年斑和Aβ的生成而起作用的。 | 朱爱琴 褚以德 LI Qiao-xin Colin L Masters | 2009 | 中国药理学通报2009，25，6： | 11 |
| 2 | 红景天对阿尔茨海默病APP-C100转基因小鼠的影响显示文摘目的 探讨红景天对 AD转基因小鼠行为的影响和抗氧化应激反应的神经保护作用。方法 用 APP- C1 0 0转基因小鼠和健康(NTg)鼠模型来观察单剂红景天 8w抗氧化效果。Open field活动用来评估转基因鼠和健康鼠的移动、探索能力及焦虑状态。 Western印迹法定量分析转基因鼠血清及大脑 Cn/ Zn SOD1、APP及 Aβ蛋白含量。免疫组织化学法观察转基因鼠大脑皮层和海马的病理改变。结果 与 NTg鼠比较 ,转基因鼠通过格子次数 (grid cross)和排泄物 (粪尿 )均显著增加 (P<0 .0 5)。通过 Western印迹法及密度定量测定 ,红景天治疗组的转基因鼠 Cn/ ZnSOD1蛋白含量在血清及大脑中较对照组明显减少 (P<0 .0 5)。大脑 APP、βCTF及 Aβ蛋白含量无变化 ,免疫组化在大脑皮层和海马未见 Aβ沉积。结论 红景天可通过减少 SOD1氧化蛋白的含量 。 | 朱爱琴 Qiao-xin Li 张鑫生 滕长青 楮以德 Colin L Masters Amee George Tian Cardamong Genevieve Evin | 2004 | 中国老年学杂志2004，24，6： | 8 |
| 3 | 藏药七十味珍珠丸对阿尔茨海默病转基因Tg2576鼠认知能力及脑羧基末端片段蛋白含量的影响显示文摘目的探讨藏药七十味珍珠丸(ratanasampil,RNSP)对阿尔茨海默病(AD)模型鼠的学习和记忆功能、认知能力以及脑β淀粉样前体蛋白(amyloid precursor protein,APP)和羧基末端片段(C-terminal fragment,CTF)蛋白含量的影响。方法应用Y-迷宫明暗分辨学习和旷场实验观察13~14月龄雌性Tg2576转基因鼠和同龄雌性BL6/SJL非转基因(NTg)鼠的学习、记忆功能和行为学的变化。治疗组选取7只Tg2576鼠和5只NTg鼠用被溶解的RNSP灌胃8周,对照组选取8只Tg2576鼠和5只NTg鼠用蒸馏水和芝麻油混匀后灌胃8周。用抗β淀粉样蛋白(Aβ)5-8多克隆抗体WO2和369抗体行蛋白印迹法测鼠血清Aβ和脑的APP和CTF(α-、β、γ-CTF)蛋白含量并进行定量分析。应用免疫组织化学法观察Aβ在鼠脑中的表达。结果治疗8周后,Y-迷宫结果显示,治疗组Tg2576鼠达标所需要的训练时间显著低于对照组Tg2576鼠[(34.2±9.7)s与(52.4±12.5)s,t=3.465,P〈0.01]。旷场试验结果显示,与对照组Tg2576鼠比较,治疗组Tg2576鼠在中央格停留时间明显减低,跨格次数和站立次数增加,排泄次数减少[(4.7±3.6)s与(12.9±9.0)s,(85.3±17.6)次与(54.3±13.9)次,(57.7±17.1)次与(20.6±17.4)次,(1.2±0.6)次与(3.4±0.9)次,t=5.871、8.200、3.093、2.231,均P〈0.05)。两对照组相比较,Tg2576鼠在中央格停留时间较NTg鼠长,跨格和站立次数较NTg鼠减少[(12.9±9.0)s与(5.2±5.9)s,(54.3±13.9)次与(85.8±22.5)次,(20.6±17.4)次与(53.5±14.0)次,均P〈0.05]。上述训练项目NTg鼠治疗组和对照组的差异均无统计学意义。蛋白印迹法结果显示,与对照组Tg2576鼠比较,RNSP治疗组Tg2576鼠血清Aβ含量显著减低(t=9.104,P〈0.01),脑组织α-CTF蛋白的水平和α-CTF/β-CTF的比值明显增高(t=20.678、14.170,均P〈0.01)。但两组脑APP、β-CTF和了γ-CTF蛋白无明显变化。免疫组化染色显示,治疗组Tg2576鼠的大脑皮质和海马周围Aβ淀粉样斑块的数目明显减少。结论RNSP能提高Tg2576鼠的学习和记忆能力。可能通过激动α分泌酶活性的APP非淀粉样酶切的代谢过程来增加鼠脑组织α—CTF蛋白的水平.降低血清Aβ浓度,使老年斑的形成减少。 | 朱爱琴 Colin L Masters LI Qiao- xin | 2009 | 中华老年医学杂志2009，28，11： | 6 |
| 4 | Polymeric foams for flexible and highly sensitive low-pressure capacitive sensors显示文摘Flexible low-pressure sensors(<10 kPa)are required in areas as diverse as blood-pressure monitoring,human–computer interactions,robotics,and object detection.For applications,it is essential that these sensors combine flexibility,high sensitivity,robustness,and low production costs.Previous works involve surface micro-patterning,electronic amplification(OFET),and hydrogels.However,these solutions are limited as they involve complex processes,large bias voltages,large energy consumption,or are sensitive to evaporation.Here,we report a major advance to solve the challenge of scalable,efficient and robust e-skin.We present an unconventional capacitive sensor based on composite foam materials filled with conductive carbon black particles.Owing to the elastic buckling of the foam pores,the sensitivity exceeds 35 kPa^(−1) for pressure<0.2 kPa.These performances are one order of magnitude higher than the ones previously reported.These materials are low-cost,easy to prepare,and display high capacitance values,which are easy to measure using low-cost electronics.These materials pave the road for the implementation of e-skin in commercialized applications. | Mickaël Pruvost Wilbert J.Smit Cécile Monteux Philippe Poulin Annie Colin | 2019 | npj Flexible Electronics2019，3，1： | 5 |
| 5 | PrP^C-related signal transduction is influenced by copper, membrane integrity and the alpha cleavage site显示文摘铜绑定,抛锚膜的、细胞的 prion 蛋白质(PrPC ) 有二个组成的劈开地点生产不同 N 终端和 C 终端碎片(N1/C1 和 N2/C2 ) 。用表示也人的 PrPC,鼠标 PrPC 或鼠标 PrPC 的 RK13 房间带 3F4 epitope,这研究在 endoproteolytic 劈开和一个通常认为的 PrPC 函数上探索了 PrPC 主要顺序的影响,地图 kinase 信号 transduction,响应外长的铜与或没有使不安的膜环境。PrPC 主要顺序,特别在 N1/C1 劈开地点附近,看起来在这个地点和细胞外的调整信号的 kinase 1/2 (ERK1/2 ) phosphorylation 影响解朊作用的基础层次,与处理激活增加与基础 ERK1/2 表明一种反的关系。人的 PrPC 独自响应铜显示出增加的 N1/C1 劈开,由特定的 p38 和 JNK/SAPK phosphorylation 伴随了。到加扣押胆固醇的抗菌素 filipin 的铜的联合暴露导致了一只老鼠信号蛋白质 phosphorylation 的 PrPC 特定的实质的增加,由 N1/C1 劈开的增加伴随了。怀有人的 N1/C1 劈开地点的老鼠 PrPC 基础地并且响应铜假定更似人类的侧面并且改变了膜环境。我们的结果证明在 N1/C1 劈开地点附近的 PrPC 主要顺序影响在这个地点处理的 endoproteolytic,它显得连接了基础地并且响应铜印射 kinase 信号 transduction。进一步,主要顺序看起来响应外长的刺激在 PrPC 相关的信号 transduction 的忠实上授与 N1/C1 劈开和膜完整的相互的依赖。 | Cathryn L Haigh | 2009 | Cell Research2009，19，9： | 4 |
| 6 | European experts consensus statement on cystic tumours of the pancreas显示文摘 | Marco Del Chiaro Caroline Verbeke Roberto Salvia Gunter Kl?ppel Jens Werner Colin McKay Helmut Friess Riccardo Manfredi Eric Van Cutsem Matthias L?hr Ralf Segersv?rd | 2013 | Digestive and Liver Disease2013，，： | 3 |
| 7 | Colonoscopy quality with Entonox? vs intravenous conscious sedation:18608 colonoscopy retrospective study显示文摘AIM To compare colonoscopy quality with nitrous oxide gas(Entonox?) against intravenous conscious sedation using midazolam plus opioid.METHODS A retrospective analysis was performed on a prospectively held database of 18608 colonoscopies carried out in Lothian health board hospitals between July 2013 and January 2016.The quality of colonoscopies performed with Entonox was compared to intravenous conscious sedation(abbreviated in this article as IVM).Furthermore,the quality of colonoscopies performed with an unmedicated group was compared to IVM.The study used the following key markers of colonoscopy quality:(1) patient comfort scores;(2) caecal intubation rates(CIRs); and (3) polyp detection rates (PDRs).We used binary logistic regression to model the data.RESULTS There was no difference in the rate of moderate-toextreme discomfort between the Entonox and IVM groups (17.9% vs 18.8%; OR = 1.06,95%CI: 0.95-1.18,P = 0.27).Patients in the unmedicated group were less likely to experience moderate-to-extreme discomfort than those in the IVM group(11.4% vs 18.8%; OR = 0.71,95%CI: 0.60-0.83,P < 0.001).There was no difference in caecal intubation between the Entonox and IVM groups(94.4% vs 93.7%; OR = 1.08,95%CI: 0.92-1.28,P = 0.34).There was no difference in caecal intubation between the unmedicated and IVM groups (94.2% vs 93.7%; OR = 0.98,95%CI: 0.79-1.22,P = 0.87).Polyp detection in the Entonox group was not different from IVM group (35.0% vs 33.1%; OR = 1.01,95%CI: 0.93-1.10,P = 0.79).Polyp detection in the unmedicated group was not significantly different from the IVM group (37.4% vs 33.1%; OR = 0.97,95%CI: 0.87-1.08,P = 0.60).CONCLUSION The use of Entonox was not associated with lower colonoscopy quality when compared to intravenous conscious sedation using midazolam plus opioid. | Alexander R Robertson Nicholas A Kennedy James A Robertson Nicholas I Church Colin L Noble | 2017 | World Journal of Gastrointestinal Endoscopy2017，9，9： | 3 |
| 8 | Global, regional, and national causes of child mortality in 2008: a systematic analysis显示文摘 | Robert E Black Simon Cousens Hope L Johnson Joy E Lawn Igor Rudan Diego G Bassani Prabhat Jha Harry Campbell Christa Fischer Walker Richard Cibulskis Thomas Eisele Li Liu Colin Mathers | 2010 | The Lancet2010，，9730： | 3 |
| 9 | Global, regional, and national causes of child mortality in 2008: a systematic analysis显示文摘 | Robert E Black Simon Cousens Hope L Johnson Joy E Lawn Igor Rudan Diego G Bassani Prabhat Jha Harry Campbell Christa Fischer Walker Richard Cibulskis Thomas Eisele Li Liu Colin Mathers | 2010 | The Lancet . 2010 (9730)2010，，： | 2 |
| 10 | 超氧化物歧化酶基因突变子对阿尔茨海默病转基因小鼠β-淀粉样蛋白表达影响的实验研究显示文摘 | 薛寿儒 Qiaoxin Li Colin L Masters | 2002 | 中华神经科杂志2002，35，4： | 2 |
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| 18 | Selective concentration of EPA and DHA using Thermomyces lanuginosus tipase is due to fatty acid selectivity and not regioselectivity显示文摘 | Taiwo O A Jacqui L A Colin J B | 2013 | Food Chemistry2013，138，： | 1 |
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