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| 1 | Peptide aptamers:The versatile role of specific protein function inhibitors in plant biotechnology显示文摘In recent years,peptide aptamers have emerged as novel molecular tools that have attracted the attention of researchers in various fields of basic and applied science,ranging from medicine to analytical chemistry.These artificial short peptides are able to specifically bind,track,and inhibit a given target molecule with high affinity,even molecules with poor immunogenicity or high toxicity,and represent a remarkable alternative to antibodies in many different applications.Their use is on the rise,driven mainly by the medical and pharmaceutical sector.Here we discuss the enormous potential of peptide aptamers in both basic and applied aspects of plant biotechnology and food safety.The different peptide aptamer selection methods available both in vivo and in vitro are introduced,and the most important possible applications in plant biotechnology are illustrated.In particular,we discuss the generation of broadbased virus resistance in crops, 'reverse genetics' and aptasensors in bioassays for detecting contaminations in food and feed.Furthermore,we suggest an alternative to the transfer of peptide aptamers into plant cells via genetic transformation,based on the use of cell-penetrating peptides that overcome the limits imposed by both crop transformation and Genetically Modified Organism commercialization. | Monica Colombo Chiara Mizzotti Simona Masiero Martin M.Kater Paolo Pesaresi | 2015 | Journal of Integrative Plant Biology2015，57，11： | 4 |
| 2 | Cytoskeleton reorganization and ultrastructural damage induced by gliadin in a three-dimensional in vitro model显示文摘AIM: To evaluate the interplay between gliadin and LoVo cells and the direct effect of gliadin on cytoskeletal patterns.METHODS: We treated LoVo multicellular spheroids with digested bread wheat gliadin in order to investigate their morphology and ultrastructure (by means of light microscopy and scanning electron microscopy), and the effect of gliadin on actin (phalloidin fluorescence)and the tight-junction protein occludin and zonula occluden-1.RESULTS: The treated spheroids had deep holes and surface blebs, whereas the controls were smoothly surfaced ovoids. The incubation of LoVo spheroids with gliadin decreased the number of intracellular actin filaments, impaired and disassembled the integrity of the tight-junction system.CONCLUSION: Our data obtained from an 'in vivolike' polarized culture system confirm the direct noxious effect of gliadin on the cytoskeleton and tight junctions of epithelial cells. Unlike two-dimensional cell culture systems, the use of multicellular spheroids seems to provide a suitable model for studying cell-cell interactions. | Ersilia Dolfini Leda Roncoroni Luca Elli Chiara Fumagalli Roberto Colombo Simona Ramponi Fabio Forlani Maria Teresa Bardella | 2005 | World Journal of Gastroenterology2005，11，48： | 3 |
| 3 | Internal Social Capital and the Attraction of Early Contributions in Crowdfunding显示文摘 | Massimo G. Colombo Chiara Franzoni Cristina Rossi‐Lamastra | 2015 | Entrepreneurship Theory and Practice2015，，1： | 2 |
| 4 | Red blood cell distribution width predicts one-year mortality following transcatheter aortic valve implantation显示文摘 | Caroline J. Magri Alaide Chieffo Azeem Latib Matteo Montorfano Francesco Maisano Michela Cioni Eustachio Agricola Remo Daniel Covello Chiara Gerli Annalisa Franco Pietro Spagnolo Ottavio Alfieri Antonio Colombo | 2014 | International Journal of Cardiology2014，，： | 1 |
| 5 | Correction of hypovitaminosis D improved global longitudinal strain earlier than left ventricular ejection fraction in cardiovascular older adults after orthopaedic surgery显示文摘BackgroundCardiovascular 疾病和维生素 D 的不够的层次是为不利外科的结果的风险因素,并且他们是在经历整形外科的外科的更老的成年人之中通常在场的两个。给维生素 D 的心血管的效果, hypovitaminosis D 的外科手术前的诊断将是为补充协议的实现的珍贵的步。如果,我们调查了浆液 25 的正规化[哦] D 能改善受不了心血管的 diseases.MethodsWe 的更老的成年人的心脏的性能注册了为主要整形外科的外科安排的 47 个更老的成年人并且受不了 hypovitaminosis D。病人们与开始的剂量经历了 6 月的 calcifediol 补充 50 湩琠敨攠敶瑮的起初手术后的白天吗? | Matteo Briguglio Luigi Gianturco Daniele Stellat Chiara Colombo Marika Bonadies Oscar Salat Mauro Anselmi Giuseppe Banff Maurizio Turiel | 2018 | Journal of Geriatric Cardiology2018，15，8： | 1 |
| 6 | WT1 overexpression at diagnosis may predict favorable outcome in patients with de novo non-M3 acute myeloid leukemia显示文摘 | Maurizio Miglino Nicoletta Colombo Gianmatteo Pica Raffaella Grasso Marino Clavio Micaela Bergamaschi Filippo Ballerini Anna Ghiso Chiara Ghiggi Laura Mitscheunig Germana Beltrami Antonia Cagnetta Luana Vignolo Maria Vita Lucchetti Sara Aquino Ivana Pierr | 2011 | Leukemia & Lymphoma2011，，10： | 1 |
| 7 | Deficiency of neutrophil phagocytosis in premature infants: effect of vitamin E supplementation 显示文摘 | Chirico G Marconi M Colombo A Chiara A Rondini G Ugazio AG | 1983 | Acta Paeditr Scand1983，72，4： | 1 |
| 8 | Aggressive Surgery in Retroperitoneal Soft Tissue Sarcoma Carried Out at High-Volume Centers is Safe and is Associated With Improved Local Control显示文摘 | Sylvie Bonvalot MD PhD Rosalba Miceli PhD Mattia Berselli MD Sylvain Causeret MD Chiara Colombo MD Luigi Mariani MD Hatem Bouzaiene MD Cécile Péchoux MD Paolo Giovanni Casali MD Axel Cesne MD Marco Fiore MD Alessandro Gronchi MD | 2010 | Annals of Surgical Oncology2010，，6： | 1 |
| 9 | Orthotopic induction of CH157MN convexity and skull base meningiomas into nude mice using stereotactic surgery and MRI characterization显示文摘Meningioma in vivo research is hampered by the difficulty of establishing an easy and reproducible orthotopic model able to mimic the characteristics of a human meningioma. Moreover, leptomeningeal dissemination and high mortality are often associated with such orthotopical models, making them useless for clinical translation studies. An optimized method for inducing meningiomas in nude mice at two different sites is described in this paper and the high reproducibility and low mortality of the models are demonstrated. Skull base meningiomas were induced in the auditory meatus and convexity meningiomas were induced on the brain surface of 23 and 24 nude mice, respectively. Both models led to the development of a mass easily observable by imaging methods. Dynamic contrast enhanced MRI was used as a tool to monitor and characterize the pathology onset and progression. At the end of the study, histology was performed to confirm the neoplastic origin of the diseased mass. | Francesca La Cava Alberto Fringuello Mingo Pietro Irrera Aldo Di Vito Alessia Cordaro Chiara Brioschi Sonia Colombo Serra Claudia Cabella Enzo Terreno Luigi Miragoli | 2019 | Animal Models and Experimental Medicine2019，2，1： | 0 |
| 10 | Cardiovascular injuries and SARS-COV-2 infection:focus on elderly people显示文摘The novel coronavirus disease(COVID-19)has hit the healthcare system worldwide.The risk of severe infection and mortality increases with advancing age,especially in subjects with comorbidities such as cardiovascular disease,hypertension,diabetes,obesity and cancer.Moreover,cardiovascular complications such as myocardial injury,heart failure and thromboembolism are frequently observed in COVID-19 cases,and several biomarkers(troponin,NTpro BNP and D-Dimer)have been identified as prognostic indicators of disease severity and worst outcome.Currently,there is no specific therapy against SARS-Co V-2,although many medications are under investigation.The aim of this review will be to explore the intertwined relationship between COVID-19 disease and the cardiovascular system,focusing on elderly population.The available supportive treatments along with the related concerns in elderly patients,due to their comorbidities and polypharmacotherapy,will be explored. | Claudia Colombo Laura Garatti Giulia Ferrante Francesca Casadei Claudio Montalto Gabriele Crimi Chiara Cogliati Enrico Ammirati Stefano Savonitto Nuccia Morici | 2021 | Journal of Geriatric Cardiology2021，18，7： | 0 |
| 11 | Use of endoscopic ultrasound-guided gallbladder drainage as a rescue approach in cases of unsuccessful biliary drainage显示文摘This narrative review provides an overview of the utilization of endoscopic ultrasound-guided gallbladder drainage(EUS-GBD)as a salvage approach in cases of unsuccessful conventional management.EUS-GBD is a minimally invasive and effective technique for drainage in patients with acute cholecystitis with high risk of surgery.The procedure has demonstrated impressive technical and clinical success rates with low rates of adverse events,making it a safe and effective option for appropriate candidates.Furthermore,EUS-GBD can also serve as a rescue option for patients who have failed endoscopic retrograde cholangiopancreatography or EUS biliary drainage for relief of jaundice in malignant biliary stricture.However,patient selection is critical for the success of EUS-GBD,and proper patient selection and risk assessment are important to ensure the safety and efficacy of the procedure.As the field continues to evolve and mature,ongoing research will further refine our understanding of the benefits and limitations of EUS-GBD,ultimately leading to improved outcomes for patients. | Alessandro Fugazza Kareem Khalaf Katarzyna M Pawlak Marco Spadaccini Matteo Colombo Marta Andreozzi Marco Giacchetto Silvia Carrara Chiara Ferrari Cecilia Binda Benedetto Mangiavillano Andrea Anderloni Alessandro Repici | 2024 | World Journal of Gastroenterology2024，30，1： | 0 |
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