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| 1 | Peptide aptamers:The versatile role of specific protein function inhibitors in plant biotechnology显示文摘In recent years,peptide aptamers have emerged as novel molecular tools that have attracted the attention of researchers in various fields of basic and applied science,ranging from medicine to analytical chemistry.These artificial short peptides are able to specifically bind,track,and inhibit a given target molecule with high affinity,even molecules with poor immunogenicity or high toxicity,and represent a remarkable alternative to antibodies in many different applications.Their use is on the rise,driven mainly by the medical and pharmaceutical sector.Here we discuss the enormous potential of peptide aptamers in both basic and applied aspects of plant biotechnology and food safety.The different peptide aptamer selection methods available both in vivo and in vitro are introduced,and the most important possible applications in plant biotechnology are illustrated.In particular,we discuss the generation of broadbased virus resistance in crops, 'reverse genetics' and aptasensors in bioassays for detecting contaminations in food and feed.Furthermore,we suggest an alternative to the transfer of peptide aptamers into plant cells via genetic transformation,based on the use of cell-penetrating peptides that overcome the limits imposed by both crop transformation and Genetically Modified Organism commercialization. | Monica Colombo Chiara Mizzotti Simona Masiero Martin M.Kater Paolo Pesaresi | 2015 | Journal of Integrative Plant Biology2015，57，11： | 4 |
| 2 | PGR5-PGRL1-Dependent Cyclic Electron Transport Modulates Linear Electron Transport Rate in Arabidopsis thaliana显示文摘植物在环境、新陈代谢的条件下面为幸存和生长需要光合的电子运输的紧密的规定。为这个目的,线性电子运输(让) 小径被很多条其他的电子转移小径和阀门补充。在 Arabidopsis,在 photosystem 附近的周期的电子运输(CET ) 我(希腊语的第二十三个字母) ,它从 ferrodoxinto plastoquinone 再循环电子,大多数被调查其他的线路。然而,互相依赖让, CET 和 CET 的相对重要性仍然保持不清楚,主要由于在对 CET 的贡献的精确评价的困难面对让,它在生理的条件下面统治电子流动。我们因此与一项最小的切开水的活动产生了 Arabidopsis 异种,并且这样低率让由在 PsbO1 联合猛烈变化, PsbP2, PsbQ1, PsbQ2,和 PsbR loci。结果 5 异种是可行的,尽管成熟叶子包含仅仅 ∼20% 野类型自然地不太丰富的 PsbO2 蛋白质。5plants 补偿减小在由增加 CET 的率,并且导致让一强壮的非光化学的熄灭(NPQ ) 反应在 dark-to-light 转变期间。识别如此的高能力的 CET 的分子的起源,我们构造了缺乏 qE 部件的三六倍的异种 NPQ (5npq4-1 ) ,调停 NDH 的 CET (5 crr4-3 ) ,或 PGR5-PGRL1-mediated CET (5 pgr5 ) 。他们的分析表明 PGR5-PGRL1-mediated CET 在 C3plants 在 pH 形成和 NPQ 的正式就职起一个主要作用。而且当 pgr5 在波动下面在幼苗舞台死时,光调节, 5 家 pgr5 工厂能幸存,它在 modulating 加重 PGR5 的重要性 intersystem 电子转移。 | Marjaana Suorsa Fabio Rossi Luca Tadini Mathias Labs Monica Colombo Peter Jahns Martin M. Kater Dario Leister Giovanni Finazzi Eva-Mari Aro Roberto Barbato Paolo Pesaresi | 2016 | Molecular Plant2016，9，2： | 3 |
| 3 | Y chromosome loss in male patients with primary biliary cirrhosis显示文摘 | Ana Lleo Sabine Oertelt-Prigione Ilaria Bianchi Lisa Caliari Palma Finelli Monica Miozzo Roberta Lazzari Annarosa Floreani Francesca Donato Massimo Colombo M. Eric Gershwin Mauro Podda Pietro Invernizzi | 2013 | Journal of Autoimmunity2013，，： | 2 |
| 4 | Short- Versus Long-Term Duration of Dual-Antiplatelet Therapy After Coronary Stenting: A Randomized Multicenter Trial显示文摘 | Marco Valgimigli Gianluca Campo Monia Monti Pascal Vranckx Gianfranco Percoco Carlo Tumscitz Fausto Castriota Federico Colombo Matteo Tebaldi Giuseppe Fucà Moh?d Kubbajeh Elisa Cangiano Monica Minarelli Antonella Scalone Caterina Cavazza Alice Frangione M | 2012 | Circulation2012，，16： | 1 |
| 5 | Microencapsulation with spray-chilling as an innovative strategy for probiotic low sodium requeijao cremoso processed cheese processing显示文摘The effect of sodium reduction and Lactobacillus acidophilus addition(free or microencapsulated)on the quality parameters of requeijao cremoso processed cheese was assessed for 90 days at 5℃.Three formulations were prepared:(CONT)regular salt content and curd fusion(90℃/2 min),(FREE)reduced salt content+free probiotic culture added after curd fusion(90℃/2 min),and(MICRO)reduced salt content+microencapsulated probiotic culture added before curd fusion(70℃/5 min).FREE and MICRO formulations showed increased monounsaturated and polyunsaturated fatty acid content,improved health indices(decreases in thrombogenic index and increases in desirable fatty acid index),and altered rheological properties(higher consistency index,apparent viscosity,elastic properties,and gel strength).In addition,the MICRO formulation showed higher texture sensory acceptance and probiotic counts higher than 6 log CFU/g during storage and simulated gastrointestinal conditions.Overall,microencapsulation of probiotics with spray chilling represents an innovative solution in requeijao cremoso processed cheese. | Ramon Silva Tatiana Colombo Pimentel Fernando Eust´aquio de Matos Junior Erick Almeida Esmerino Monica Q.Freitas Carmen Silvia F´avaro-Trindade M´arcia Cristina Silva Adriano Gomes Cruz | 2022 | Food Bioscience2022，46，2： | 0 |
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