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| 1 | A Pid3 allele from rice cultivar Gumei2 confers resistance to Magnaporthe oryzae显示文摘瑞斯强风,由 Magnaporthe oryzae 引起了,是大多数破坏疾病之一。用基于地图的策略并且在 silico 途径,我们孤立新米饭(Oryza sativa L.) Pid3 的强风抵抗等位基因,指明的 Pi25,从稳定的强风抵抗栽培变种 Gumei2。在表示上分析和互补测试证明 Pi25 授与强风抵抗到 M。oryzae 孤立 js001-20。顺序分析证明 Pi25 是有与在核苷酸位置的 Pid3 相比的单个核苷酸替换的 2772 核苷酸的 intronless 基因 459 并且述词地编码了典型卷的 coilnucleotide 绑定 siteleucine 有到 Pid3 的 100% 身份的 924 氨基酸剩余的富有的重复(CCNBSLRR ) 蛋白质通常认为的蛋白质。在 Nipponbare 的易受影响的等位基因 pi25 在核苷酸位置包含了一个胡说八道变化 2209 与 736 氨基酸剩余导致截断的蛋白质。另外,导致 10 氨基酸替换的 14 核苷酸替换在 Pi25 和在上游的 pi25 之间被识别在易受影响的等位基因的早熟的站 codon。尽管 Pi25/Pid3-mediated 抵抗的机制需要进一步被调查,等位基因的隔离将在经由转基因的途径引起节目的米饭强风抵抗便于 Pi25/Pid3 的利用,标记帮助选择。 | Jie Chen Yongfeng Shi Wenzheng Liu Rongyao Chai Yaping Fu Jieyun Zhuang Jianli Wu | 2011 | Journal of Genetics and Genomics2011，38，5： | 45 |
| 2 | 5-Hydroxymethylcytosine signatures in circulating cell-free DNA as diagnostic biomarkers for human cancers显示文摘象 5-methylcytosine (5mC ) 和 5-hydroxymethylcytosine (5hmC ) 那样的 DNA 修正是知道在哺乳动物影响全球基因表示的 epigenetic 标记。在人的染色体,与基因表示的靠近的关联和高化学的稳定性给他们的流行,这些 DNA epigenetic 标记能为癌症用作理想的 biomarkers 诊断。利用一种高度敏感、选择的化学标记技术,我们这里报导在传播没有房间的 DNA ( cfDNA )并且在从最近与 colorectal 诊断的 260 个病人的一个队收集的配对的肿瘤和邻近的纸巾的 genomic DNA ( gDNA )的 5hmC 的染色体宽的介绍,胃,胰腺,从 90 个健康个人的肝或甲状腺癌症和正常纸巾。5hmC 主要在与开的染色质和容许的 histone 修正重合的 transcriptionally 活跃的区域被散布。柔韧的联系癌症的 5hmC 签名在 cfDNA 被识别为特定的癌症类型是特征的。传播 cfDNA 的 5hmC 底 biomarkers colorectal 和胃的癌症是高度预兆的并且比常规 biomarkers 优异并且比得上从织物活体检视的 5hmC biomarkers。因此,这新策略能从血样品的分析为癌症的诊断和预后导致有效、最低限度地侵略的方法的发展。 | Wenshuai Li Xu Zhang Xingyu Lu Lei You Yanqun Song Zhongguang Luo Jun Zhang Ji Nie Wanwei Zheng Diannan Xu Yaping Wang Yuanqiang Dong Shulin Yu Jun Hong Jianping Shi Hankun Hao Fen Luo Luchun Hua Peng Wang Xiaoping Qian Fang Yuan Lianhuan Wei Ming Cui Taiping Zhang Quan Liao Menghua Dai Ziwen Liu Ge Chen Katherine Meckel Sarbani Adhikari Guifang Jia Marc B Bissonnette Xinxiang Zhang Yupei Zhao Wei Zhang Chuan He Jie Liu | 2017 | Cell Research2017，27，10： | 30 |
| 3 | Genetic analysis of 'all-fish' growth hormone gene transferred carp (Cyprinus carpio L.) and its F_1 generation显示文摘Recornbinant 'all-fish' growth hormone gene (GH) was microinjected into the fertilized eggs of carp. A comparison between the growth traits of transgenics and non-transgenics was carried out, and the transgenic individuals with significant 'fast-growing' effect were successfully gained. A comparison on the reproductivities was also given out between the transgenics and their non-transgenic siblings, and showed that the reproductive capacity of transgenics was substantially equivalent to those of the non-transgenics. On the other hand, the genetic separation and the characteristic distribution of the FI generation were genetically analyzed, which gave solid evidence for the hypothesis that 2-3 chromosomes are integrated with trans-gene. In addition, the distinct biological effects for multi-site-integrated transgenes were further discussed. The present study opens a door for the breeding of 'fast-growing' transgenic fish. | WANG Yaping, HU Wei, WU Gang,SUN Yonghua, CHEN Shangping, ZHANG Fuying,ZHU Zuoyan, FENG Jianxin & ZHANG Xirui1. State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China 2. Henan Institute of Aquaculture, Zhengzhou 450044, China | 2001 | Chinese Science Bulletin2001，46，14： | 23 |
| 4 | Overexpression of SGR Results in Oxidative Stress and Lesion-mimic Cell Death in Rice Seedlings显示文摘It is thought that the Stay Green Rice(SGR)gene is involved in the disaggregation of the light harvesting complex and in the subsequent breakdown of chlorophyll and apo-protein during senescence.In this study,we found that overexpression of SGR(Ov-SGR)resulted in the generation of singlet oxygen and other reactive oxygen species and produced a chlorophyll-dependent regional cell death phenotype on leaves of rice seedlings.Transcriptome analyses using Affymetrix Rice GeneChips revealed that Ov-SGR rice seedlings exhibited a number of signs of singlet oxygen response.The genes and their associated biochemical pathways identified provide an insight into how rice plants respond to singlet oxygen at the molecular and physiologic level. | Huawu Jiang Yaping Chen Meiru Li Xingian Xu Guojiang Wu | 2011 | Journal of Integrative Plant Biology2011，53，5： | 16 |
| 5 | Aquaporin-dependent excessive intrauterine fluid accumulation is a major contributor in hyper-estrogen induced aberrant embryo implantation显示文摘 | Ying Zhang Qi Chen He Zhang Qiang Wang Rong Li Yaping Jin Haibin Wang Tonghui Ma Jie Qiao Enkui Duan | 2015 | Cell Research2015，25，1： | 14 |
| 6 | Myelin Oligodendrocyte Glycoprotein-IgG Contributes to Oligodendrocytopathy in the Presence of Complement, Distinct from Astrocytopathy Induced by AQP4-IgG显示文摘Immunoglobulin G against myelin oligodendrocyte glycoprotein(MOG-Ig G) is detectable in neuromyelitis optica spectrum disorder(NMOSD) without aquaporin-4 Ig G(AQP4-Ig G), but its pathogenicity remains unclear.In this study, we explored the pathogenic mechanisms of MOG-Ig G in vitro and in vivo and compared them with those of AQP4-Ig G. MOG-Ig G-positive serum induced complement activation and cell death in human embryonic kidney(HEK)-293 T cells transfected with human MOG. In C57 BL/6 mice and Sprague-Dawley rats, MOG-Ig G only caused lesions in the presence of complement. Interestingly, AQP4-Ig G induced astroglial damage, while MOGIg G mainly caused myelin loss. MOG-Ig G also induced astrocyte damage in mouse brains in the presence ofcomplement. Importantly, we also observed ultrastructural changes induced by MOG-Ig G and AQP4-Ig G. These findings suggest that MOG-Ig G directly mediates cell death by activating complement in vitro and producing NMOSDlike lesions in vivo. AQP4-Ig G directly targets astrocytes,while MOG-Ig G mainly damages oligodendrocytes. | Ling Fang Xinmei Kang Zhen Wang Shisi Wang Jingqi Wang Yifan Zhou Chen Chen Xiaobo Sun Yaping Yan Allan G. Kermode Lisheng Peng Wei Qiu | 2019 | Neuroscience Bulletin2019，35，5： | 13 |
| 7 | Genetic and biological characteristics of the globally circulating H5N8 avian influenza viruses and the protective efficacy offered by the poultry vaccine currently used in China显示文摘The H5N8 avian influenza viruses have been widely circulating in wild birds and are responsible for the loss of over 33 million domestic poultry in Europe, Russia, Middle East, and Asia since January 2020. To monitor the invasion and spread of the H5N8 virus in China, we performed active surveillance by analyzing 317 wild bird samples and swab samples collected from 41,172 poultry all over the country. We isolated 22 H5N8 viruses from wild birds and 14 H5N8 viruses from waterfowls. Genetic analysis indicated that the 36 viruses formed two different genotypes: one genotype viruses were widely detected from different wild birds and domestic waterfowls;the other genotype was isolated from a whopper swan. We further revealed the origin and spatiotemporal spread of these two distinct H5N8 virus genotypes in 2020 and 2021. Animal studies indicated that the H5N8 isolates are highly pathogenic to chickens, mildly pathogenic in ducks, but have distinct pathotypes in mice. Moreover, we found that vaccinated poultry in China could be completely protected against H5N8 virus challenge. Given that the H5N8 viruses are likely to continue to spread in wild birds, vaccination of poultry is highly recommended in high-risk countries to prevent H5N8 avian influenza. | Pengfei Cui Xianying Zeng Xuyong Li Yanbing Li Jianzhong Shi Conghui Zhao Zhiyuan Qu Yanwen Wang Jing Guo Wenli Gu Qi Ma Yuancheng Zhang Weipeng Lin Minghui Li Jingman Tian Dongxue Wang Xin Xing Yanjing Liu Shuxin Pan Yaping Zhang Hongmei Bao Liling Liu Guobin Tian Chengjun Li Guohua Deng Hualan Chen | 2022 | Science China(Life Sciences)2022，65，4： | 12 |
| 8 | High efficient gene targeting in rice field eel Monopterus albus by transcription activator-like effector nucleases显示文摘Genome editing plays an important role in the research of gene functions,gene correction and cell replacement therapy.Traditionally,genome editing is mainly dependent on the technology of homologous recombination(HR)and embryonic stem(ES)cell lines in mammals[1].However,genome editing is severely limited by the low efficiency of HR and lack of ES cell lines in most organisms.Recently,the development of zinc finger nucleases(ZFNs), | Ke Feng Hongrui Luo Yongming Li Ji Chen Yaping Wang Yonghua Sun Zuoyan Zhu Wei Hu | 2017 | Science Bulletin2017，62，3： | 9 |
| 9 | Induction and transmission of wheat-Haynaldia villosa chromosomal translocations显示文摘In order to develop more wheat-Haynaldia villosa translocations involving different chromosomes and chromosome segments of H. villosa, T. durum-H. villosa amphiploid was irradiated with 60Co γ-rays at doses of 800, 1,200, and 1,600 rad. Pollen collected from the spikes 1, 2, and 3 days after irradiation were transferred to emasculated spikes of the common wheat cv. ‘Chinese Spring'. Genomic in situ hybridization was used to identify wheat-H. villosa chromosome translocations in the M1 generation. Transmission of the identified translocation chromosomes was analyzed in the BC1, BC2, and BC3 generations. The results indicated that all three irradiation doses were highly efficient for inducing wheat-alien translocations without affecting the viability of the M1 seeds. Within the range of 800-1,600 rad, both the efficiency of translocation induction and the frequency of interstitial chromosome breakage-fusion increased as the irradiation dosage increased. A higher translocation induction frequency was observed using pollen collected from the spikes 1 day after irradiation over that of 2 or 3 days after irradiation. More than 70% of the translocations detected in the M1 generation were transmitted to the BC1 through the female gametes. All translocations recovered in the BC1 generation were recovered in the following BC2, and BC3 generations. The transmission ability of different translocation types in different genetic backgrounds showed an order of ‘whole-arm translocation > small alien segment translocation > large alien segment translocation', through either male or female gametes. In general, the transmission ability through the female gametes was higher than that through the male gametes. By this approach, 14 translocation lines that involved different H. villosa chromosomes have been identified in the BC3 using EST-STS markers, and eight of them were homozygous. | Yaping Cao Tongde Bie Xiue Wang Peidu Chen | 2009 | Journal of Genetics and Genomics2009，36，5： | 8 |
| 10 | Metamorphism and zircon U–Pb dating of garnet amphibolite in the Baoyintu Group, Inner Mongolia显示文摘Garnet amphibolite in the Baoyintu Group,Inner Mongolia, is mainly composed of garnet, hornblende,plagioclase, quartz and minor rutile/ilmenite. Garnet occurs as porphyroblasts surrounded by plagioclase, forming typical ‘‘white-eye socket'' texture. Garnet shows grossular content of 0.26–0.28 and pyrope of 0.11–0.13, without significant zoning. Plagioclase is generally zoned with anorthite(An) increasing from core to rim. The P–T pseudosection calculated using THERMOCALC in the system Mn NCKFMASHTO shows that the garnet amphibolite experienced a clockwise P–T path with a peak at*1.3 GPa/725 °C defined from the minimum An content in plagioclase and maximum pyrope content in garnet,followed by an isothermal decompression. LA-ICP-MS zircon dating for the garnet amphibolite indicates metamorphic ages of 399 ± 6 Ma. The peak P–T condition corresponds to a thermal gradient of *18 °C/km, indicating typical medium-pressure type that commonly occurs in orogenic process with crustal thickening. We prefer to interpret this orogenic event to be a result of collision,following the closure of the Paleo-Asian Ocean before theearly Devonian although there is a popular view that the Paleo-Asian Ocean may have lasted to the early Mesozoic. | Yaping Chen Chunjing Wei Jinrui Zhang Hang Chu | 2015 | Science Bulletin2015，60，19： | 8 |
| 11 | Matrilineal History of the Rana longicrus Species Group(Rana,Ranidae,Anura) and the Description of a New Species from Hunan,southern China显示文摘Fieldwork in Jiemuxi National Nature Reserve,Hunan,China,discovered morphologically indistinguishable groups of brown frogs that bred at different,exclusive times of the year.A principal components analysis of morphometric data,molecular analyses,and the exclusive breeding season suggested the occurrence of two species.The population that breeds during the winter was found to be an undescribed species and was subsequently described as Rana jiemuxiensis sp.nov.The new cryptic species can be identified from its congeners at the same locality by having a different breeding season and a divergent DNA barcode.Six major lineages of brown frogs were resolved with high nodal support.Rana japonica,R.chaochiaoensis,R.omeimontis,and R.jiemuxiensis have independent maternal lineages.Rana hanlunica and R.maoershanensis have essentially identical maternal lineages and they appear to represent the same species.The validity of the species R.longicrus,R.zhenhaiensis,and R.culaiensis and their genealogical relationships are not resolved and deserve further study.The genealogy indicates that sympatric cryptic lineages probably widely exist in the R.longicrus group.This highlights the importance of future more fine-scaled samplings and the inclusion of nuclear genes. | Fang YAN Ke JIANG Hongman CHEN Ping FANG Jieqiong JIN Yi LI Shaoneng WANG Robert W.MURPHY Jing CHE Yaping ZHANG | 2011 | Asian Herpetological Research2011，2，2： | 8 |
| 12 | Accumulation and Output of Heavy Metals by the Invasive Plant Spartina alterniflora in a Coastal Salt Marsh显示文摘Spartina alterniflora is a foreign introduced species and has far-reaching effects on salt marsh ecosystems, particularly on the biogeochemical cycle of heavy metals. To ascertain whether the invasive plant Spartina alterniflora Loisel is a source of metals in the environment, we determined the bimonthly concentrations of heavy metals, chromium(Cr), lead(Pb), copper(Cu), zinc(Zn),and manganese(Mn), in the roots, stems, and leaves of S. alterniflora from a typical semidiurnal tidal zone in the coastal area of northern Jiangsu Province, China. Based on the measurements, we calculated annual metal primary accumulation and output. To calculate the annual output of heavy metals from S. alterniflora, a new method that calculates the annual rate of biomass loss and decomposition was developed. The annual primary accumulation of Cr, Pb, Cu, Zn, and Mn was 19.08, 84.19, 63.74, 442.58, and774.66 mg m^(-2), respectively, and the annual output from S. alterniflora to the surrounding environment was 4.01, 18.09, 14.00, 97.11,and 164.28 mg m^(-2), respectively. Spartina alterniflora only provides temporary storage, and its absorption of heavy metals could be used to remediate contaminated soil and for phytomining. The heavy metals released by S. alterniflora to the environment cannot be ignored; thus, S. alterniflora should be considered a source of metal contamination. Therefore, when we evaluate the remarkable ability of certain plant species to concentrate metals in their tissues, the balance between heavy metal accumulation and output should be considered. | CHEN Lian GAO Jianhua ZHU Qingguang WANG Yaping YANG Yang | 2018 | Pedosphere2018，28，6： | 7 |
| 13 | Alpha-2-macroglobulin as a radioprotective agent: a review显示文摘Radiation is an important modality in cancer treatment, and eighty percent of cancer patients need radiotherapy at some point during their clinical management. However, radiation-induced damage to normal tissues restricts the therapeutic doses of radiation that can be delivered to tumours and thereby limits the effectiveness of the treatment. The use of radioprotectors represents an obvious strategy to obtain better tumour control using a higher dose in radiotherapy. However, most of the synthetic radioprotective compounds studied have shown inadequate clinical efficacy owing to their inherent toxicity and high cost. Hence, the development of radioprotective agents with lower toxicity and an extended window of protection has attracted a great deal of attention, and the identification of alternative agents that are less toxic and highly effective is an absolute necessity. Recent studies have shown that alpha-2-macroglobulin(α2M) possesses radioprotective effects. α2M is a tetrameric, disulfide-rich plasma glycoprotein that functions as a nonselective inhibitor of different types of non-specific proteases and as a carrier of cytokines, growth factors, and hormones. α2M induces protein factors whose interplay underlies radioprotection, which supports the idea that α2M is the central effector of natural radioprotection in the rat. Pretreatment with α2M has also induced a significant reduction of irradiation-induced DNA damage and the complete restoration of liver and body weight. Mihailovi? et al. concluded that the radioprotection provided by α2M was in part mediated through cytoprotection of new blood cells produced in the bone marrow; these authors also indicated that an important aspect of the radioprotective effect of amifostine was the result of the induction of the endogenous cytoprotective capability of α2M. The radioprotective effects of α2M are possibly due to antioxidant, antifibrosis, and anti-inflammatory functions, as well as the maintenance of homeostasis, and enhancement of the DNA repair and cell recovery processes. This review is the first to summarise the observations and elucidate the possible mechanisms responsible for the beneficial effects of α2M. The lacunae in the existing knowledge and directions for future research are also addressed. | Xueying Chen Xiangbo Kong Zhaoqiang Zhang Wei Chen Jieyu Chen Huanyang Li Wanting Cao Yaping Ge Silian Fang | 2014 | Chinese Journal of Cancer Research2014，26，5： | 7 |
| 14 | Similarity theory for the physical simulation of natural gas hydrate reservoir development显示文摘In order to apply physical simulation results to natural gas hydrate reservoir parameters to provide a theoretical framework for the design of a development plan,an analytical equation method was used to obtain the similarity criteria of natural gas hydrate reservoir development by physical simulation,based on a mathematical model of natural gas hydrate development.Given the approach of numerical simulation,a sensitivity analysis for all parameters was carried out,which specifically demonstrated that initial temperature is the most important parameter.Parameters of thermal conductivity coefficients are not necessary for conducting the NGH dissociation process,which will fundamentally simplify the design and establishment of the model.The analysis provides a sound theoretical basis and design principles for particular similarity. | LIU Yaping CHEN Yueming BAI Yuhu LI Shuxia | 2010 | Mining Science and Technology2010，20，5： | 7 |
| 15 | CFD–DEM–CVD multi-physical field coupling model for simulating particle coating process in spout bed显示文摘Particle coating is a very important step in many industrial production processes as the particle coating layers may fix surfaces with unique advantages. Given the limitation and disadvantages of the existing simulation methods, a coupled CFD–DEM–CVD multi-physical field model for particle-coating simulations has been established taking into account the velocity field, temperature field, concentration field, and deposition model. In this model, gas behavior and chemical reactions are simulated in the CFD frame based on the conservation laws of mass, momentum, and energy. The particle behavior is simulated in the DEM frame based on the gas–solid interphase force model and contact force model. The deposition behavior is simulated in the CVD frame based on the particle movement–adhesion–deposition model. The coupled model can be implemented in Fluent-EDEM software with their user definition function and application programming interface. The particle coating process involving the pyrolysis of acetylene was investigated, and the effect of bed temperature and inlet gas velocity on deposition rate and coating efficiency were investigated based on the proposed model with adjustable deposition coefficients. Both the average deposition layer mass and the average deposition layer thickness were found to be proportional to the elapsed time and increased at the rate of about 1.05 × 10^-2 mg/s and 3.45 × 10^-4 mm/s, respectively, setting the inlet gas velocity to 11 m/s and bed temperature to 1680 K. A higher temperature and larger inlet gas velocity lead to a larger deposition rate, but the coating efficiency decreases because of limits imposed by the chemical reaction. At a bed temperature of 1280 K, the average deposition rate is 7.40 × 10?3 mg/s when the inlet gas velocity is set to 11 m/s, which is about double the deposition rate when the inlet gas velocity is set as 5 m/s. The proposed model can provide some guidance for the operating conditions and parameters design of the spouted bed in actual coating settings and can also be further developed as a basic model of mechanisms to integrate detailed information across multiple scales. | Malin Liu Meng Chen Tianjin Li Yaping Tang Rongzheng Liu Youlin Shao Bing Liu Jiaxing Chang | 2019 | Particuology2019，17，1： | 6 |
| 16 | Oleic Acid and Eicosapentaenoic Acid Reverse Palmitic Acid-induced Insulin Resistance in Human HepG2 Cells via the Reactive Oxygen Species/JUN Pathway显示文摘Oleic acid(OA),a monounsaturated fatty acid(MUFA),has previously been shown to reverse saturated fatty acid palmitic acid(PA)-induced hepatic insulin resistance(IR).However,its underlying molecular mechanism is unclear.In addition,previous studies have shown that eicosapentaenoic acid(EPA),aω-3 polyunsaturated fatty acid(PUFA),reverses PA-induced muscle IR,but whether EPA plays the same role in hepatic IR and its possible mechanism involved need to be further clarified.Here,we confirmed that EPA reversed PA-induced IR in HepG2 cells and compared the proteomic changes in HepG2 cells after treatment with different free fatty acids(FFAs).A total of 234 proteins were determined to be differentially expressed after PA+OA treatment.Their functions were mainly related to responses to stress and endogenous stimuli,lipid metabolic process,and protein binding.For PA+EPA treatment,the PA-induced expression changes of 1326 proteins could be reversed by EPA,415 of which were mitochondrial proteins,with most of the functional proteins involved in oxidative phosphorylation(OXPHOS)and tricarboxylic acid(TCA)cycle.Mechanistic studies revealed that the protein encoded by JUN and reactive oxygen species(ROS)play a role in OA-and EPA-reversed PA-induced IR,respectively.EPA and OA alleviated PA-induced abnormal adenosine triphosphate(ATP)production,ROS generation,and calcium(Ca^(2+))content.Importantly,H_(2)O_(2)-activated production of ROS increased the protein expression of JUN,further resulting in IR in HepG2 cells.Taken together,we demonstrate that ROS/JUN is a common response pathway employed by HepG2 cells toward FFA-regulated IR. | Yaping Sun Jifeng Wang Xiaojing Guo Nali Zhu Lili Niu Xiang Ding Zhensheng Xie Xiulan Chen Fuquan Yang | 2021 | Genomics, Proteomics & Bioinformatics2021，19，5： | 6 |
| 17 | Far-field super-resolution imaging by nonlinearly excited evanescent waves显示文摘Abbe’s resolution limit,one of the best-known physical limitations,poses a great challenge for any wave system in imaging,wave transport,and dynamics.Originally formulated in linear optics,the Abbe limit can be broken using nonlinear optical interactions.We extend the Abbe theory into a nonlinear regime and experimentally demonstrate a far-field,label-free,and scan-free super-resolution imaging technique based on nonlinear four-wave mixing to retrieve near-field scattered evanescent waves,achieving a sub-wavelength resolution ofλ∕5.6.This method paves the way for numerous new applications in biomedical imaging,semiconductor metrology,and photolithography. | Zhihao Zhou Wei Liu Jiajing He Lei Chen Xin Luo Dongyi Shen Jianjun Cao Yaping Dan Xianfeng Chen Wenjie Wan | 2021 | Advanced Photonics2021，3，2： | 6 |
| 18 | Antigenic analysis of grass carp reovirus using single-chain variable fragment antibody against IgM from Ctenopharyngodon idella显示文摘Grass carp (Ctenopharyngodon idella) is an important species of freshwater aquaculture fish in China. However, grass carp reovirus (GCRV) can cause fatal hemorrhagic disease in yearling populations. Until now, a strategy to define the antigenic capacity of the virus's structural proteins for preparing an effective vaccine has not been available. In this study, some single-chain variable fragment antibodies (scFv), which could specifically recognize grass carp IgM, were selected from a constructed mouse na ve antibody phage display cDNA library. The identified scFv C1B3 clone was shown to possess relatively higher specific binding activity to grass carp IgM. Furthermore, ELISA analysis indicated that the IgM level in serum from virus-infected grass carp was more than two times higher than that of the control group at 5-7 days post infection. Moreover, Western blot analysis demonstrated that the outer capsid protein VP7 has a specific immuno-binding-reaction with the serum IgM from virus-infected grass carp. Our results suggest that VP7 can induce a stronger immune response in grass carp than the other GCRV structural proteins, which implies that VP7 protein could be used as a preferred immunogen for vaccine design. | CHEN CongLin SUN XiaoYun LIAO LanJie LUO ShaoXiang LI ZhouQuan ZHANG XiaoHua WANG YaPing GUO QionLin FANG Qin DAI HePing | 2013 | Science China(Life Sciences)2013，56，1： | 6 |
| 19 | Highly efficient Zr doped-TiO_2/glass fiber photocatalyst and its performance in formaldehyde removal under visible light显示文摘Zr-doped-TiO_2 loaded glass fiber(ZT/GF) composite photocatalysts with different Zr/Ti ratios were prepared with a sol–gel process. Zr4+can replace Ti4+in the TiO_2 lattice, which is conducive to forming the anatase phase and reducing the calcination temperature. The glass fiber carrier was responsible for better dispersion and loading of Zr-doped-TiO_2 particles, improving the applicability of the Zr-doped-TiO_2. The ZT/GF photocatalysts were characterized by X-ray diffraction(XRD), scanning electron microscope(SEM), Fourier transform infrared spectroscopy(FT-IR), ultraviolet–visible spectroscopy(UV–vis) and Barrett–Joyner–Halenda(BJH). The performance of photocatalysts with different loading was evaluated in formaldehyde degradation under visible light at room temperature. ZT/GF0.2exhibited the highest activity, with a formaldehyde removal rate as high as 95.14% being observed, which is better than that of the photocatalyst particles alone. The stability of the catalyst was also tested, and ZT/GF exhibited excellent catalytic performance with 94.38%removal efficiency, even after seven uses. | Chao Huang Yaping Ding Yingwen Chen Peiwen Li Shemin Zhu Shubao Shen | 2017 | Journal of Environmental Sciences2017，29，10： | 6 |
| 20 | Quantitative Proteomics Using Isobaric Labeling:A Practical Guide显示文摘In the past decade,relative proteomic quantification using isobaric labeling technology has developed into a key tool for comparing the expression of proteins in biological samples.Although its multiplexing capacity and flexibility make this a valuable technology for addressing various biological questions,its quantitative accuracy and precision still pose significant challenges to the reliability of its quantification results.Here,we give a detailed overview of the different kinds of isobaric mass tags and the advantages and disadvantages of the isobaric labeling method.We also discuss which precautions should be taken at each step of the isobaric labeling workflow,to obtain reliable quantification results in large-scale quantitative proteomics experiments.In the last section,we discuss the broad applications of the isobaric labeling technology in biological and clinical studies,with an emphasis on thermal proteome profiling and proteogenomics. | Xiulan Chen Yaping Sun Tingting Zhang Lian Shu Peter Roepstorff Fuquan Yang | 2021 | Genomics, Proteomics & Bioinformatics2021，19，5： | 5 |
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