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| 1 | Experimental measurement of the electrical conductivity of single crystal olivine at high temperature and high pressure under different oxygen fugacities显示文摘At 1.0-4.0 GPa and 1123-1473 K and under oxygen fugacity-controlled conditions (Ni + NiO, Fe + Fe3O4, Fe + FeO and Mo+ M0O2 buffers), a YJ-3000t Model six-anvil solid high-pressure apparatus and a Sarltron-1260 Impedance/Gain-Phase analyzer were employed to conduct an in situ measurement of the electrical conductivity of single crystal olivine. Experimental results showed that: (1) within the range of experimentally selected frequencies (103-106 Hz), the electrical conductivity of the sample is of great dependence on the frequency; (2) with the rise of temperature ( T), the electrical conductivity (σ) will increase, and the Arrenhius linear relationship is established between lgσ and 1/T; (3) under the control of oxygen buffer Fe+ Fe3O4, with the rise of pressure, the electrical conductivity tends to decrease whereas the activation enthalpy and independent-of-temperature preexponential factor tend to increase, with the activation energy and activation volume of the sample estimated at (1. 25 ± 0.08) eV and (0.105 ±0.025) cm3/mol, respectively; (4) under given pressure and temperature conditions, the electrical conductivity tends to increase whereas the activation energy tends to decrease with increasing oxygen fugacity; and ( 5) the mechanism of electrical conduction of small polarons can provide insight into the behavior of electrical conduction of olivine under high pressure and high temperature. | DAI Lidong LI Heping LIU Congqiang CUI Tongdi SHAN Shuangming YANG Changjun LIU Qingyou DENG Heming | 2006 | Progress in Natural Science:Materials International2006，16，4： | 9 |
| 2 | Antigenic analysis of grass carp reovirus using single-chain variable fragment antibody against IgM from Ctenopharyngodon idella显示文摘Grass carp (Ctenopharyngodon idella) is an important species of freshwater aquaculture fish in China. However, grass carp reovirus (GCRV) can cause fatal hemorrhagic disease in yearling populations. Until now, a strategy to define the antigenic capacity of the virus's structural proteins for preparing an effective vaccine has not been available. In this study, some single-chain variable fragment antibodies (scFv), which could specifically recognize grass carp IgM, were selected from a constructed mouse na ve antibody phage display cDNA library. The identified scFv C1B3 clone was shown to possess relatively higher specific binding activity to grass carp IgM. Furthermore, ELISA analysis indicated that the IgM level in serum from virus-infected grass carp was more than two times higher than that of the control group at 5-7 days post infection. Moreover, Western blot analysis demonstrated that the outer capsid protein VP7 has a specific immuno-binding-reaction with the serum IgM from virus-infected grass carp. Our results suggest that VP7 can induce a stronger immune response in grass carp than the other GCRV structural proteins, which implies that VP7 protein could be used as a preferred immunogen for vaccine design. | CHEN CongLin SUN XiaoYun LIAO LanJie LUO ShaoXiang LI ZhouQuan ZHANG XiaoHua WANG YaPing GUO QionLin FANG Qin DAI HePing | 2013 | Science China(Life Sciences)2013，56，1： | 6 |
| 3 | Expression of reconstructive hF Ⅷ in the hrDNA by using hrDNA targeting vector显示文摘Our lab has constructed a new nonviral vec-tor—hrDNA targeting vector(pHrneo). pHrneo is a human derived vector that can target gene into human ribosomal DNA(hrDNA) locus. In this study, we inserted expression cassette of reconstructive hF Ⅷ (hFVIII-BDDAK39) to pHrneo to construct targeting vector: pHrneo-BDDAK39. Through electroporation of pHrneo-BDDAK39 into HT1080 cells, we identified the homologous recombinants by PCR and Southen blotting, and tested the expression of hFVIII- BDDAK39 in the hrDNA locus. The hFⅧ-BDDAK39 was successfully targeted into hrDNA locus of HT-1080 by pHrneo-BDDAK39, and the efficiency of site-specific inte-gration was 2.0×10?5. hFⅧ-BDDAK39 in hrDNA locus of HT-1080 is found to be able to express efficiently (32±5 ng·106 cells?1·24 h?1). Targeting vector pHrneo-BDDAK39 can find use in gene therapy for hemophilia. | LIU Xionghao LIU Mujun SHE Hua WEN Lu XUE Zhigang LIANG Desheng CAI Fang PAN Qian LONG Zhigao WU Lingqian DAI Heping XIA Kun XlA Jiahui | 2005 | Chinese Science Bulletin2005，50，19： | 5 |
| 4 | Experimental Study on the Electrical Conductivity of Orthopyroxene at High Temperature and High Pressure under Different Oxygen Fugacities显示文摘At presure 1.0–4.0 GPa and temperature 1073–1423 K and under oxygen partial pressure conditions, a YJ-3000t multi-anvil solid high-pressure apparatus and Sarltron-1260 Impedance/Gain-Phase analyzer were employed toconduct an in-situ measurement of the electrical conductivity of orthopyroxene. The buffering reagents consist of Ni+NiO,Fe+Fe3O4, Fe+FeO and Mo+MoO2 in order to control the environmental oxygen fugacity. Experimental results made clearthat: (1) within the measuring frequency range from 10? to 106 Hz, the complex impedance (R) is of intensive dependence 1on the frequency; (2) The electrical conductivity (σ) tends to increase along to the rise of temperature (T), and Log σ vs. 1/T fit the Arrenhius linear relations; (3) Under the control of oxygen buffer Fe+Fe3O4, with the rise of pressure, theactivation enthalpy tends to increase whereas the electrical conductivity tends to decrease. The activation energy andactivation volume of the main current carriers of orthopyroxene have been obtained, which are (1.715±0.035) eV and (0.03±0.01) cm3/mol, respectively; (4) Under given pressure and temperature, the electrical conductivity tends to increase withincreasing oxygen fugacity, while under given pressure the activation enthalpy tends to decrease with increasing oxygenfugacity; and (5) The sample’s small polarons mechanism has provided a reasonable explanations to the conductionbehavior at high temperature and high pressure. | DAI Lidong LI Heping LIU Congqiang SHAN Shuangming CUI Tongdi SU Genli | 2005 | Acta Geologica Sinica(English Edition)2005，79，6： | 4 |
| 5 | Investigation of hrDNA targeting vector-mediated tumor-specific suicide gene therapy for hepatocellular carcinoma显示文摘Human ribosomal DNA (hrDNA) target- ing vector (pHrn) is one of the human derived vectors, which was devised by our lab and has got patent authority. To investigate its effect on gene therapy for hepatocellular carcinoma, a double suicide fusion gene expression cassette, CDUPRT/GFP controlled by a synthetic CMV enhancer-enhanced hTERT promoter (CeTp) which was determined by luciferase assays was constructed in pHrn backbone, creating an expression vector pHr-CeTpCDUPRT/GFP. After transfer of plasmid to hepatocellular carcinoma cell line Bel7402 in vitro, the transfection efficiency reached 30%―50% by using flow cytometer. The expression of CDUPRT/GFP was detected by RT-PCR and Western Blotting. After the administra- tion of 5-FC, high performance liquid chromatography (HPLC) was applied to examine the level of 5-FU in supernatant, resulting in a concentration of 60.15 μg/mL. The Methylthiazolyl tetrazolium (MTT) assay was then utilized to investigate the antitumor effect of pHr-CeTpCDUPRT/GFP in Bel7402 cells, and rela- tive cell survival of 60%-35% was observed after 5-FC treatment. In vivo experiments, the nude mouse model of hepatocellular carcinoma was constructed and in situ gene therapy was performed. The results indicated the tumor growth of treatment group was obviously suppressed, and some even shrank, whenthe vectors and prodrugs were injected continuously. The expression of CDUPRT in tumor tissues was also identified by RT-PCR, and the concentration of 5-FU was 7.694 μg/mL in blood serum using HPLC detection. Then the pathological section of tumor tissues revealed significant tumor cell necrosis. All of these results provide important experiment evidences of the gene therapy for hepatocellular carcinoma with the utilization of our vectors. | WANG Lina XUE Zhigang LI Zhuo XUE Jinfeng LIU Xionghao PAN Qian LONG Zhigao CAI Fang WU Lingqian DAI Heping XIA Kun LIANG Desheng XIA Jiahui | 2006 | Chinese Science Bulletin2006，51，19： | 3 |
| 6 | Functional analysis of three genetic disorder related PITX2 mutants显示文摘The autosome dominant disorders, ring dermoid of the cornea (RDC), iris hypolasia (IH) and Axenfeld-Rieger syndrome (ARS), are allelic disor- ders, as all three can result from mutations of the transcriptional factor PITX2. Among three disorder phenotypes, ARS is the most severe, IH is milder than ARS, and RDC is the mildest. Missense muta- tions of the PITX2 homeodomain identified in RDC (R62H), IH (R84W) and ARS patients (T68P) were introduced into PITX2 cDNA by site-directed mutagenesis. PITX2 mutant proteins expressed in eucaryotic cells were stable and localized to the nu- cleus. Analysis of these mutant PITX2 proteins by DNA-binding shift and transactivation studies dem- onstrated that the R62H had the most activity in both studies, and the R84W still retained somewhat func- tions, whereas the T68P proved to be non-functional. These results are consistent with previous hypothesis that varying amount of residual PITX2 mutant activity could underline the severity of these phenotypes. | WANG Guo LIU Xiaoping PAN Qian LIANG Desheng WU Qianling DAI Heping ZHU Feizhou XIA Kun XIA Jiahui | 2006 | Chinese Science Bulletin2006，51，2： | 3 |
| 7 | Role of JAK-STAT3 signaling pathway during neuronal differentiation of rat bone marrow mesenchymal stem cells显示文摘BACKGROUND: Recent studies regarding neuronal differentiation of mesenchymal stem cells (MSCs) have primarily focused on induction methods and transplantation in vivo.However,knowledge about the intrinsic regulatory mechanisms underlying neuronal induction of MSCs remains limited and unclear.OBJECTIVE: To elucidate the role of JAK-STAT3 signaling pathway during neuronal differentiationof MSCs using a combination of the JAK-STAT3 signaling inhibitor AG490 and growth factors.DESIGN,TIME AND SETTING: Neural,molecular,biomedical,in vitro experiment was performed at the Laboratory of Pharmacology,School of Pharmacy,Nanjing Medical University between Marchand December 2008 MATERIALS: An inhibitor of the JAK-STAT3 signaling pathway was purchased from Calbiochem,USA.Antibody kit for total and phosphorylated STAT3 was purchased from Cell Signaling,USA.METHODS: MSCs from passage 3 were assigned to non-induced,growth factor,and AG490 groups.MAIN OUTCOME MEASURE: The number of cells expressing neuron-specific enolase,microtubule-associated protein,and glial fibrillary acidic protein were determined by immunocytochemistry.Total and phosphorylated (Tyr705) expression levels of STAT3 protein were measured by Western blot analysis.RESULTS: MSCs were transdifferentiated into neuronaland astrocyte-like phenotypes throughthe induction of epidermal growth factor,basic fibroblast growth factor,and brain-derived neurotrophic factor.In addition,the JAK-STAT3 signaling pathway was significantly activated during neural differentiation.Expression of phosphorylated (Tyr705) STAT3 was inhibited with AG490 (5 μmol/L) prior to neural induction with epidermal growth factor,basic fibroblast growth factor,and brain-derived neurotrophic factor;proportion of astrocyte-like cells was significantly reduced (P < 0.01),and the proportion of neuronal-like phenotypes was significantly increased (P < 0.01).CONCLUSION: JAK-STAT3 signaling pathway was shown to regulate neuronal induction of bone marrow MSCs.The proportion of MSC-induced neuronal-like cells was increased following treatment with the JAK-STAT3 signaling inhibitor AG490. | Huaiyong Hao Yongguang Wang Feng Cheng Baosheng Huang Heping Tian Xueliang Dai Lixin Li | 2010 | Neural Regeneration Research2010，5，5： | 2 |
| 8 | Silica nanoparticle is a possible safe carrier for gene therapy显示文摘In order to develop a safe and effective gene therapy carrier, some toxicological and biodynamical ex- periments were carried out on silica nanoparticles (SiNPs). First we prepared SiNPs with appropriate portions of cyclo- hexane, deionized water and ethyl silicate, and then trans- fected the modified SiNPs and GFP plasmid DNA complex into the HT1080 cells to test the effectiveness of transfection for gene therapy. At the same time, we injected the SiNPs into a number of mice through tail vein. Then we made the mice crossed to evaluate the acute, long-term and reproduc- tive toxicity. In vivo distribution analysis and pathological examination were made on both adult mice and their off- spring. SiNPs were uniform and had an average diameter of 40 nm, and the modified SiNPs carried exogenous DNA molecules into target cells and the transferred GFP fusion gene was effectively expressed in the cells. The SiNPs injected via tail vein were widely distributed in almost all of tissues, and the injected mice had the ability to reproduce normally. The in vivo and in vitro results of this study clearly show that SiNPs can be used as a safe and effective carrier for gene transfection and gene therapy. | XUE Zhigang LIANG Desheng LI Yumei LONG Zhigao PAN Qian LIU Xionghao WU Lingqian ZHU Shaihong CAI Fang DAI Heping TANG Baisha XIA Kun XIA Jiahui | 2005 | Chinese Science Bulletin2005，50，20： | 2 |
| 9 | Identification and characterization of human neuronal voltage-gated calcium channel gamma 3 subunit gene显示文摘By homologous expressed sequence tag (EST) searching, one EST (GenBank: W29095) was obtained, which shows 75% identity in 435 bp overlap with the coding sequence of mouse Cacng2 gene. A 1 545 bp cDNA fragment was obtained from the nested polymerase chain reaction (PCR) and rapid applification of cDNA end (RACE) reaction in the human brain prefrontal cortex cDNA library and the human brain Ready cDNA with the primers designed on W29095. The fragment contained a 948-bp open reading frame (ORF) encoding 315 amino acids, and was named CACNG3. As it was identical to a BAC clone (GenBank: AC004125) from chromosome 16p12-p13.1, the CACNG3 gene was mapped to human chromosome 16p12-p13.1, and the coding region was composed of 4 exons. Reverse transcription PCR (RT-PCR) analysis showed that the CACNG3 gene expressed in human adult brain and fetal brain. Single strand comformation polymorphism (SSCP) analysis was performed in 3 pedigrees with autosomal recessive retinitis pigmentosa, 8 pedigrees with autosomal | Jiahui Xia Huali Zhang Dongsheng Tang Xixiang Tang Heping Dai Qian Pan Zhigao Long Xiaodong Liao | 2000 | Chinese Science Bulletin2000，45，23： | 2 |
| 10 | Investigation Of Soft-Switching Techniques For Power Electronics Building Blocks(PEBB)显示文摘 | Kun Xing Fred C Lee | 1998 | Proceedings of APEC1998，，： | 1 |
| 11 | Northwestern Junggar Basin, Xiemisitai Mountains, China: A geochemical and geochronological approach显示文摘 | Ping Shen Yuanchao Shen Xian-Hua Li Hongdi Pan Heping Zhu Lei Meng Huawu Dai | 2012 | LITHOS2012，，： | 1 |
| 12 | High-pressure investigations on the isostructural phase transition and metallization in realgar with diamond anvil cells显示文摘The high-pressure structural,vibrational and electrical properties for realgar were investigated by in-situ Raman scattering and electrical conductivity experiments combined with first-principle calculations up to~30.8 GPa.It was verified that realgar underwent an isostructural phase transition at~6.3 GPa and a metallization at a higher pressure of~23.5 GPa.The isostructural phase transition was well evidenced by the obvious variations of Raman peaks,electrical conductivity,crystal parameters and the As–S bond length.The phase transition of metallization was in closely associated with the closure of bandgap rather than caused by the structural phase transition.And furthermore,the metallic realgar exhibited a relatively low compressibility with the unit cell volume V_(0)=718.1.4Å^(3)and bulk modulus B_(0)=36.1 GPa. | Linfei Yang Lidong Dai Heping Li Haiying Hu Meiling Hong Xinyu Zhang Pengfei Liu | 2021 | Geoscience Frontiers2021，12，2： | 1 |
| 13 | Construction and Characterization of a Novel Recombinant Single-Chain Variable Fragment Antibody against White Spot Syndrome Virus from Shrimp显示文摘 | Dai Heping Gao Hong Zhao Xinyan etal | 2003 | Journal of Immunological Methods2003，279，： | 1 |
| 14 | Grain Boundary Conductivities of Peridotite at High Temperature and High Pressure显示文摘The grain boundary conductivities of dry synthetic peridotite are conducted by virtue of YJ-3000t multi-anvil press and Solartron-1260 Impedance /Gain-phase Analyzer at conditions of 4.0 GPa, 1373 K and frequency ranging from 10-2 to 106 Hz. The Fe2O3+Fe3O4,Ni+NiO,Fe+Fe3O4,Fe+FeO and Mo+MoO2 solid buffers are adopted to adjust | Lidong Dai~1,Heping Li~1,Haiying Hu~(1,2),Shuangming Shan~1 1.Laboratory for Study of the Earth’s Interior and Geofluids,Institute of Geochemistry,Chinese Academy of Sciences,Guiyang 550002,China. 2.Graduate School of the Chinese Academy of Sciences,Beijing 100080,China | 2009 | 地学前缘2009，16，S1： | 0 |
| 15 | Isolation by phage display and characterization of a single-chain antibody specific for O^6-methyldeoxyguanosine显示文摘New approaches of making single chain Fv antibodies against O6-methyl-2’ -deoxyguanosine (O6MdG) have been demonstrated by using the phage antibody display system. Using O6MdG as an antigen, 21 positive clones were identified by ELISA from this library, one of which, designated H3, specifically binds to O6MdG with high affinity. The H3 scFv antibody has an affinity constant (Kaff) of 5.94×1011(mol/L)-1. H3 scFv has been successfully used to detect O6MdG in DNA hydrolyses from yeast or E. coli cells treated with a DNA methylating agent. To our knowledge, this is the first report of the selection of a specific scFv against DNA adducts. The results demonstrate the potential applications of the phage display technology for the detection of DNA lesions caused by mutagens and carcinogens. | DAI Heping ZHAO Rouhong Haug Brenda Sean M.Hemmingsen XIAO Wei | 2001 | Chinese Science Bulletin2001，46，12： | 0 |
| 16 | Development of a pressure coring system for the investigation of deep underground exploration显示文摘To provide a more accurate evaluation of the scale of deep underground resources,a new pressure coring system was carefully developed and its strength and safety of the engineering were verified by theoretical analyses and numerical simulation.The designed pressure coring system can obtain cores with length of 3 m and diameter of 50 mm at 70 MPa.The results of the ball-drop operation experiment demonstrate that differential motion assembly can effectively cut a safety pin by applying a tensile force of 4852 N,and it can lift the core tube through the center pole to complete a series of mechanical actions to seal the pressure.Additionally,by maintaining pressures at 70 MPa for 30 min,the pressure sealing capacity of the system was proven.Furthermore,a core sample with a diameter of 50 mm was obtained through a core drilling experiment and the coring performance of the pressure coring system was verified.This study can not only enrich the existing onshore coring technology but also provide a theoretical guide and design criteria for the development of similar pressure coring systems to meet the demand for deeper underground exploration. | Da Guo Ling Chen Zhongya Zhou Dingming Wang Yiwei Zhang Xun Yang Xin Fang Xiaojun Wang Heping Lu Lin Dai Heping Xie | 2023 | International Journal of Mining Science and Technology2023，33，11： | 0 |
| 17 | 标准以外的高功率PoE解决方案显示文摘 | Heping Dai David Arciniega | 2004 | 电子产品世界2004，11，08B： | 0 |
| 18 | The Influence of Dehydration on the Electrical Conductivity of Trachyandesite at High Temperatures and High Pressures显示文摘 | Lidong Dai Keshi Hui Wenqing Sun Haiying Hu Heping Li Jianjun Jiang | 2017 | 材料科学与工程(中英文A版)2017，7，5： | 0 |
| 19 | Electrical properties of dry polycrystalline olivine mixed with various chromite contents: Implications for the high conductivity anomalies in subduction zones显示文摘Chromite,a crucial high-conductivity mineral phase of peridotite in ophiolite suites,has a significant effect on the electrical structure of subduction zones.The electrical conductivities of sintered polycrystalline olivine containing various volume percents of chromite(0,4,7,10,13,16,18,21,23,100 vol.%)were measured using a complex impedance spectroscopic technique in the frequency range of 10^(−1)-10^(6) Hz under the conditions of 1.0-3.0 GPa and 873-1223 K.The relationship between the conductivities of the chromite-bearing olivine aggregates and temperatures conformed to the Arrhenius equation.The positive effect of pressure on the conductivities of the olivine-chromite systems was much weaker than that of temperature.The chromite content had an important effect on the conductivities of the olivine-chromite systems,and the bulk conductivities increased with increasing volume fraction of chromite to a certain extent.The inclusion of 16 vol.%chromites dramatically enhanced the bulk conductivity,implying that the percolation threshold of interconnectivity of chromite in the olivine-chromite systems is-16 vol.%.The fitted activation enthalpies for pure polycrystalline olivine,polycrystalline olivine with isolated chromite,polycrystalline olivine with interconnected chromites,and pure polycrystalline chromite were 1.25,0.78-0.87,0.48-0.54,and 0.47 eV,respectively.Based on the chemical compositions and activation enthalpies,small polaron conduction was proposed to be the dominant conduction mechanism for polycrystalline olivine with various chromite contents.Furthermore,the conductivities of polycrystalline olivine with interconnected chromite(10-1.5-100.5 S/m)provides a reasonable explanation for the high conductivity anomalies in subduction-related tectonic environments. | Wenqing Sun Jianjun Jiang Lidong Dai Haiying Hu Mengqi Wang Yuqing Qi Heping Li | 2021 | Geoscience Frontiers2021，12，5： | 0 |
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