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| 1 | aunalysis of the Genome Sequence of the Medicinal Plant Salvia miltiorrhiza显示文摘 | Haibin Xu Jingyuan Song Hongmei Luo Yujun Zhang Qiushi Li Yingjie Zhu Jiang Xu Ying Li Chi Song Bo Wang Wei Sun Guoan Shen Xin Zhang Jun Qian Aijia Ji Zhichao Xu Xiang Luo Liu He Chuyuan Li Chao Sun Haixia Yah Guanghong Cui Xiwen Li Xian 'en Li Jianhe Wei Juyan Liu Yitao Wang Alice Hayward David Nelson Zemin Ning Reuben J. Peters Xiaoquan Qi Shilin Chen | 2016 | Molecular Plant2016，9，6： | 70 |
| 2 | New alkylamides from pericarps of Zanthoxylum bungeanum显示文摘二新不饱和的 alkylamides (2E,7E,9E )-N-(2-hydroxy-2-methylpropyl)-6,11-dioxo-2,7,9-dodecatrienamide (1 ) 并且(2E,6E,8E ) 和六已知的混合物(38 ) 的 -N-(2-hydroxy-2-methylpropyl)-10-oxo-2,6,8-decatrienamide (2 ) 从 Zanthoxylum bungeanum 的果皮被孤立。他们的结构根据广泛的分光镜的分析被阐明,包括 MS,红外, 1D 和 2D NMR 实验。 | Shuai Huang Lei Zhao Xian Li Zhou Mei Ying Cui Juan Wang Jie Weng | 2012 | Chinese Chemical Letters2012，23，11： | 21 |
| 3 | Effect of icarisid II on diabetic rats with erectile dysfunction and its potential mechanism via assessment of AGEs, autophagy, roTOR and the NO-cGMP pathway显示文摘可勃起的机能障碍(编辑) 是糖尿病 mellitus 的主要复杂并发症。Icariin 被显示了通过它的 bioactive 形式提高可勃起的功能, icarisid II。这研究经由对先进 glycosylation 结束的评价与编辑和它的潜在的机制在糖尿病的老鼠上调查 icarisid II 的效果产品(年龄) , autophagy, mTOR 和 NO-cGMP 小径。Icarisid II 被一个酶的方法从 icariin 提取。在控制和糖尿病的编辑组,老鼠被管理正常盐;在 icarisid II 组,老鼠 intragastrically 是管理 icarisid II。可勃起的功能被测量 intracavernosal 压力 / 平均数评估动脉的压力(ICP/MAP ) 。年龄集中,氮的氧化物 synthase (NOS ) 活动和 cGMP 集中被酶免疫分析估计。房间增长被分析使用甲基 thiazolyl tetrazolium 试金和流动 cytometry。Autophagosomes 被传播电子显微镜学, monodansylcadaverine 染色和 GFP-LC3 本地化观察。在 autophagy 的 NOS isoforms 和关键蛋白质的表示被西方的污点检验。我们的结果证明了 Icarisid II 增加了 ICP/MAP 价值,光滑的肌肉房间(SMC ) 生长曲线, S 阶段和 SMC/collagen 纤丝(SMC/CF ) 比例和减少的 Beclin 1 (P< 0.05 ) 。Icarisid II 显著地增加了 proliferative 索引和 p-p70S6K (Thr389 ) 层次并且减少 autophagosomes 的数字和 LC3-II 的层次(P< 0.01 ) 。Icarisid II 减少了年龄集中和增加的 cGMP 集中, NOS 活动(P< 0.05 ) 并且 cNOS 层次(P< 0.01 ) 在糖尿病的编辑组。因此, Icarisid II 组成有希望的混合物因为糖尿病的编辑和力量涉及 SMC 增长和 NO-cGMP 小径和年龄, autophagy 和 mTOR 小径的 downregulation 的 upregulation。 | Jian Zhang Ai-Min Li Bao-Xing Liu Fei Han Feng Liu Shao-Peng Sun Xin Li Shu-Jin Cui Shao-Zhong Xian Guang-Qi Kong Zhong-Cheng Xin Zhi-Li Ji | 2013 | Asian Journal of Andrology2013，15，1： | 17 |
| 4 | The efficiency of long-term straw return to sequester organic carbon in Northeast China's cropland显示文摘Black soil is one of the most precious soil resources on earth because it has abundant carbon stocks and a relatively high production capacity. However, decreasing organic matter after land reclamation, and the effects of long-term inputs of organic carbon have made it less fertile black soil in Northeast China. Straw return could be an effective method for improving soil organic carbon(SOC) sequestration in black soils. The objective of this study was to evaluate whether straw return effectively increases SOC sequestration. Long-term field experiments were conducted at three sites in Northeast China with varying latitudes and SOC densities. Study plots were subjected to three treatments: no fertilization(CK); inorganic fertilization(NPK); and NPK plus straw return(NPKS). The results showed that the SOC stocks resulting from NPKS treatment were 4.0 and 5.7% higher than those from NPK treatment at two sites, but straw return did not significantly affect the SOC stocks at the third site. Furthermore, at higher SOC densities, the NPKS treatment resulted in significantly higher soil carbon sequestration rates(CSR) than the NPK treatment. The equilibrium value of the CSR for the NPKS treatment equated to cultivation times of 17, 11, and 8 years at the different sites. Straw return did not significantly increase the SOC stocks in regions with low SOC densities, but did enhance the C pool in regions with high SOC densities. These results show that there is strong regional variation in the effects of straw return on the SOC stocks in black soil in Northeast China. Additional cultivations and fertilization practices should be used when straw return is considered as an approach for the long-term improvement of the soil organic carbon pool. | WANG Shi-chao ZHAO Ya-wen WANG Jin-zhou ZHU Ping CUI Xian HAN Xiao-zeng XU Ming-gang LU Chang-ai | 2018 | Journal of Integrative Agriculture2018，17，2： | 17 |
| 5 | Roles of sphincter of Oddi motility and serum vasoactive intestinal peptide,gastrin and cholecystokinin octapeptide显示文摘AIM:To investigate roles of sphincter of Oddi(SO)motility played in pigment gallbladder stone formation in model of guinea pigs.METHODS:Thirty-four adult male Hartley guinea pigs were divided randomly into two groups:the control group and pigment stone group.The pigment stone group was divided into 4 subgroups with 6 guinea pigs each according to time of sacrifice,and were fed a pigment lithogenic diet and sacrificed after 3,6,9 and 12wk.SO manometry and recording of myoelectric activity of the guinea pigs were obtained by multifunctional physiograph at each stage.Serum vasoactive intestinal peptide(VIP),gastrin and cholecystokinin octapeptide (CCK-8)were detected at each stage in the process of pigment gallbladder stone formation by enzyme-linked immunosorbent assay.RESULTS:The incidence of pigment gallstone formation was 0%,0%,16.7%and 66.7%in the 3-,6-,9-and 12-wk group,respectively.The frequency of myoelectric activity decreased in the 3-wk group.The amplitude of myoelectric activity had a tendency to decrease but not significantly.The frequency of the SO decreased significantly in the 9-wk group.The SO basal pressure and common bile duct pressure increased in the 12-wk group(25.19±7.77 mmHg vs 40.56±11.81 mmHg,22.35±7.60 mmHg vs 38.51±11.57mmHg,P<0.05).Serum VIP was significantly elevated in the 6-and 12-wk groups and serum CCK-8 was decreased significantly in the 12-wk group.CONCLUSION:Pigment gallstone-causing diet may induce SO dysfunction.The tension of the SO increased.The disturbance in SO motility may play a role in pigment gallstone formation,and changes in serum VIP and CCK-8 may be important causes of SO dysfunction. | Zhen-Hai Zhang Cheng-Kun Qin Shuo-Dong Wu Jian Xu Xian-Ping Cui Zhi-Yi Wang Guo-Zhe Xian | 2014 | World Journal of Gastroenterology2014，20，16： | 12 |
| 6 | Insight-HXMT observations of the first binary neutron star merger GW170817显示文摘Finding the electromagnetic(EM) counterpart of binary compact star merger, especially the binary neutron star(BNS) merger,is critically important for gravitational wave(GW) astronomy, cosmology and fundamental physics. On Aug. 17, 2017,Advanced LIGO and Fermi/GBM independently triggered the first BNS merger, GW170817, and its high energy EM counterpart,GRB 170817 A, respectively, resulting in a global observation campaign covering gamma-ray, X-ray, UV, optical, IR, radio as well as neutrinos. The High Energy X-ray telescope(HE) onboard Insight-HXMT(Hard X-ray Modulation Telescope) is the unique high-energy gamma-ray telescope that monitored the entire GW localization area and especially the optical counterpart(SSS17 a/AT2017 gfo) with very large collection area(~1000 cm^2) and microsecond time resolution in 0.2-5 MeV. In addition,Insight-HXMT quickly implemented a Target of Opportunity(ToO) observation to scan the GW localization area for potential X-ray emission from the GW source. Although Insight-HXMT did not detect any significant high energy(0.2-5 MeV) radiation from GW170817, its observation helped to confirm the unexpected weak and soft nature of GRB 170817 A. Meanwhile,Insight-HXMT/HE provides one of the most stringent constraints(~10^(-7) to 10^(-6) erg/cm^2/s) for both GRB170817 A and any other possible precursor or extended emissions in 0.2-5 MeV, which help us to better understand the properties of EM radiation from this BNS merger. Therefore the observation of Insight-HXMT constitutes an important chapter in the full context of multi-wavelength and multi-messenger observation of this historical GW event. | TiPei Li ShaoLin Xiong ShuangNan Zhang FangJun Lu LiMing Song XueLei Cao Zhi Chang Gang Chen Li Chen TianXiang Chen Yong Chen YiBao Chen YuPeng Chen Wei Cui WeiWei Cui JingKang Deng YongWei Dong YuanYuan Du MinXue Fu GuanHua Gao He Gao Min Gao MingYu Ge YuDong Gu Ju Guan ChengCheng Guo DaWei Han Wei Hu Yue Huang Jia Huo ShuMei Jia LuHua Jiang WeiChun Jiang Jing Jin YongJie Jin Bing Li ChengKui Li Gang Li MaoShun Li Wei Li Xian Li XiaoBo Li XuFang Li YanGuo Li ZiJian Li ZhengWei Li XiaoHua Liang JinYuan Liao CongZhan Liu GuoQing Liu HongWei Liu ShaoZhen Liu XiaoJing Liu Yuan Liu YiNong Liu Bo Lu XueFeng Lu Tao Luo Xiang Ma Bin Meng Yi Nang JianYin Nie Ge OU JinLu Qu Na Sai Liang Sun Yin Tan Lian Tao WenHui Tao YouLi Tuo GuoFeng Wang HuanYu Wang Juan Wang WenShuai Wang YuSa Wang XiangYang Wen BoBing WU Mei Wu GuangCheng Xiao He Xu YuPeng Xu LinLi Yan JiaWei Yang Sheng Yang YanJi Yang AiMei Zhang ChunLei Zhang ChengMo Zhang Fan Zhang HongMei Zhang Juan Zhang Qiang Zhang Shu Zhang Tong Zhang Wei Zhang WanChang Zhang WenZhao Zhang Yi Zhang Yue Zhang YiFei Zhang YongJie Zhang Zhao Zhang ZiLiang Zhang HaiSheng Zhao JianLing Zhao XiaoFan Zhao ShiJie Zheng Yue Zhu YuXuan Zhu ChangLin Zou | 2018 | Science China(Physics,Mechanics & Astronomy)2018，61，3： | 12 |
| 7 | The enhanced X-ray Timing and Polarimetry mission—eXTP显示文摘In this paper we present the enhanced X-ray Timing and Polarimetry mission—eXTP. eXTP is a space science mission designed to study fundamental physics under extreme conditions of density, gravity and magnetism. The mission aims at determining the equation of state of matter at supra-nuclear density, measuring effects of QED, and understanding the dynamics of matter in strong-field gravity. In addition to investigating fundamental physics, eXTP will be a very powerful observatory for astrophysics that will provide observations of unprecedented quality on a variety of galactic and extragalactic objects. In particular, its wide field monitoring capabilities will be highly instrumental to detect the electro-magnetic counterparts of gravitational wave sources.The paper provides a detailed description of:(1) the technological and technical aspects, and the expected performance of the instruments of the scientific payload;(2) the elements and functions of the mission, from the spacecraft to the ground segment. | ShuangNan Zhang Andrea Santangelo Marco Feroci YuPeng Xu FangJun Lu Yong Chen Hua Feng Shu Zhang Sφren Brandt Margarita Hernanz Luca Baldini Enrico Bozzo Riccardo Campana Alessandra De Rosa YongWei Dong Yuri Evangelista Vladimir Karas Norbert Meidinger Aline Meuris Kirpal Nandra Teng Pan Giovanni Pareschi Piotr Orleanski QiuShi Huang Stephane Schanne Giorgia Sironi Daniele Spiga Jiri Svoboda Gianpiero Tagliaferri Christoph Tenzer Andrea Vacchi Silvia Zane Dave Walton ZhanShan Wang Berend Winter Xin Wu Jean J.M.in't Zand Mahdi Ahangarianabhari Giovanni Ambrosi Filippo Ambrosino Marco Barbera Stefano Basso Jörg Bayer Ronaldo Bellazzini Pierluigi Bellutti Bruna Bertucci Giuseppe Bertuccio Giacomo Borghi XueLei Cao Franck Cadoux Francesco Ceraudo TianXiang Chen Yu Peng Chen Jerome Chevenez Marta Civitani Wei Cui WeiWei Cui Thomas Dauser Ettore Del Monte Sergio Di Cosimo Sebastian Diebold Victor Doroshenko Michal Dovciak YuanYuan Du Lorenzo Ducci QingMei Fan Yannick Favre Fabio Fuschino JoséLuis Ga'lvez Min Gao MingYu Ge Olivier Gevin Marco Grassi QuanYing Gu YuDong Gu DaWei Han Bin Hong Wei Hu Long Ji ShuMei Jia WeiChun Jiang Thomas Kennedy Ingo Kreykenbohm Irfan Kuvvetli Claudio Labanti Luca Latronico Gang Li MaoShun Li Xian Li Wei Li ZhengWei Li Olivier Limousin HongWei Liu XiaoJing Liu Bo Lu Tao Luo Daniele Macera Piero Malcovati Adrian Martindale Malgorzata Michalska Bin Meng Massimo Minuti Alfredo Morbidini Fabio Muleri Stephane Paltani Emanuele Perinati Antonino Picciotto Claudio Piemonte JinLu Qu Alexandre Rachevski Irina Rashevskaya Jerome Rodriguez Thomas Schanz ZhengXiang Shen LiZhi Sheng JiangBo Song LiMing Song Carmelo Sgro Liang Sun Ying Tan Phil Uttley Bo Wang DianLong Wang GuoFeng Wang Juan Wang LangPing Wang YuSa Wang Anna L.Watts XiangYang Wen Jörn Wilms ShaoLin Xiong JiaWei Yang Sheng Yang YanJi Yang Nian Yu WenDa Zhang Gianluigi Zampa Nicola Zampa Andrzej A.Zdziarski AiMei Zhang ChengMo Zhang Fan Zhang Long Zhang Tong Zhang Yi Zhang XiaoLi Zhang ZiLiang Zhang BaoSheng Zhao ShiJie Zheng Yu Peng Zhou Nicola Zorzi J.Frans Zwart | 2019 | Science China(Physics,Mechanics & Astronomy)2019，62，2： | 11 |
| 8 | Clinical and experimental study of effect of Raondix Salviae Militiorhiza and other blood-activating and stasis-eliminating Chinese herbson hemodynamics of portal hypertension显示文摘INTRODUCTIONTherehasbeennolonglastingandsideeffectsfreedrugstolowertheportalhypertensioninpatientswithlivercirrhosissofar.Th... | YAO Xi Xian, CUI Dong Lai, SUN Yu Feng and LI Xiao Tian | 1998 | World Journal of Gastroenterology1998，4，5： | 10 |
| 9 | Malnutrition and Quality of Life in Chinese Cancer Patients: a Clinical Study of 23,994 Subjects显示文摘Background Malnutrition is common in patients with cancer,and can negatively impact their quality of life(QoL)and even survival.However,there is currently no large data available on the prevalence of malnutrition in Chinese cancer patients.This study evaluated the prevalence of malnutrition and the QoL of Chinese patients with locoregional,recurrent or metastatic cancer.Methods We conducted a nationwide observational,multi-center,hospital-based cross-sectional study within the Chinese Society of Nutritional Oncology(CSNO)Network.All of the patients were diagnosed with one of the following 18 different types of malignant tumors:lung cancer,gastric cancer,liver cancer,colorectal cancer,breast cancer,esophageal cancer,cervical cancer,endometrial cancer,nasopharyngeal carcinoma,malignant lymphoma,leukemia,pancreatic cancer,ovarian cancer,prostate cancer,bladder cancer,brain cancer,biliary tract malignant tumors or gastrointestinal stromal tumors.These patients were enrolled from 72 hospitals located in different regions of China.The patients’nutritional status was evaluated based on the body mass index(BMI),loss of bodyweight,laboratory measurements and patient generated-subjective global assessment(PG-SGA)scores.The cancer patients’physical status and QoL were assessed by the Karnofsky Performance Status(KPS)questionnaire and the European Organization for Research and Treatment of Cancer(EORTC)QLQ-C30 questionnaire,respectively.Results From December 2013 to April 2016,23,994 patients hospitalized for cancer treatment(such as surgery,chemotherapy or radiotherapy)were enrolled in the study.The patients included 12,494(52.9%)males and 11,124(47.1%)females.The mean age was 55.8±13.7 years.The proportions of patients in cancer stagesⅠ,Ⅱ,Ⅲ,Ⅳand uncertain were 11.5%,20.3%,27.5%,30.2%and 10.5%,respectively.Among the 23,994 inpatients,the proportions of patients who were underweight(BMI<18.5 kg/m2),normal(18.5 kg/m2 | Hong Xia Xu Chun Hua Song Zhen Ming Fu Chang Wang Zeng Qing Guo Yuan Lin Ying Ying Shi Wen Hu Yi Ba Su Yi Li Zeng Ning Li Kun Hua Wang Jing Wu Ying He Jia Jun Yang Cong Hua Xie Fu Xiang Zhou Xin Xia Song Gong Yan Chen Wen Jun Ma Su Xia Luo Zi Hua Chen Ming Hua Cong Hu Ma Chun Ling Zhou Wei Wang Qi Luo Yong Mei Shi Yu Mei Qi Hai Ping Jiang Wen Xian Guan Jun Qiang Chen Jia Xin Chen Yu Fang Lan Zhou Yong Dong Feng Rong Shao Tan Tao Li Jun Wen Ou Qing Chuan Zhao Jian Xiong Wu Min Weng Qing Hua Yao Jiu Wei Cui Wei Li Han Ping Shi the Investigation on Nutrition Status and Clinical Outcome of Common Cancers(INSCOC)Group | 2021 | Journal of Nutritional Oncology2021，6，1： | 8 |
| 10 | Endoscopic full-thickness resection and laparoscopic surgery for treatment of gastric stromal tumors显示文摘AIM: To assess the effectiveness of endoscopic fullthickness resection(EFR) and laparoscopic surgery in the treatment of gastric stromal tumors arising from the muscularis propria.METHODS: Out of 62 gastric stromal tumors arising from the muscularis propria, each > 1.5 cm in diameter, 32 were removed by EFR, and 30 were removed by laparoscopic surgery. The tumor expression of CD34, CD117, Dog-1, S-100, and SMA was assessed immunohistochemically. The operative time, complete resection rate, length of hospital stay, incidence of complications, and recurrence rate were compared between the two groups. Continuous data were compared using in-dependent samples t-tests, and categorical data were compared using χ2 tests.RESULTS: The 32 gastric stromal tumors treated by EFR and the 30 treated by laparoscopic surgery showed similar operative time [20-155 min(mean, 78.5 ± 30.1 min) vs 50-120 min(mean, 80.9 ± 46.7 min), P > 0.05], complete resection rate(100% vs 93.3%, P > 0.05), and length of hospital stay [4-10 d(mean, 5.9 ± 1.4 d) vs 4-19 d(mean, 8.9 ± 3.2 d), P >0.05]. None of the patients treated by EFR experienced complications, whereas two patients treated by laparoscopy required a conversion to laparotomy, and one patient had postoperative gastroparesis. No recurrences were observed in either group. Immunohistochemical staining showed that of the 62 gastric stromal tumors diagnosed by gastroscopy and endoscopic ultrasound, six were leiomyomas(SMA-positive), one was a schwannoglioma(S-100 positive), and the remaining 55 were stromal tumors.CONCLUSION: Some gastric stromal tumors arising from the muscularis propria can be completely removed by EFR. EFR could likely replace surgical or laparoscopic procedures for the removal of gastric stromal tumors. | Liu-Ye Huang Jun Cui Cheng-Rong Wu Bo Zhang Li-Xin Jiang Xiang-Shu Xian Shu-Juan Lin Ning Xu Xiao-Ling Cao Zhi-Hua Wang | 2014 | World Journal of Gastroenterology2014，20，25： | 7 |
| 11 | Organic photovoltaic cell with 17% efficiency and superior processability显示文摘The development of organic photoactive materials,especially the newly emerging non-fullerene electron acceptors(NFAs),has enabled rapid progress in organic photovoltaic(OPV)cells in recent years.Although the power conversion efficiencies(PCEs)of the top-performance OPV cells have surpassed 16%,the devices are usually fabricated via a spin-coating method and are not suitable for large-area production.Here,we demonstrate that the fine-modification of the flexible side chains of NFAs can yield 17%PCE for OPV cells.More crucially,as the optimal NFA has a suitable solubility and thus a desirable morphology,the high efficiencies of spin-coated devices can be maintained when using scalable blade-coating processing technology.Our results suggest that optimization of the chemical structures of the OPV materials can improve device performance.This has great significance in larger-area production technologies that provide important scientific insights for the commercialization of OPV cells. | Yong Cui Huifeng Yao Ling Hong Tao Zhang Yabing Tang Baojun Lin Kaihu Xian Bowei Gao Cunbin An Pengqing Bi Wei Ma Jianhui Hou | 2020 | National Science Review2020，7，7： | 7 |
| 12 | Genetic Analysis and Rescue of a Triple-reassortant H3N2 Influenza A Virus Isolated From Swine in Eastern China显示文摘One influenza H3N2 virus, A/swine/Shandong/3/2005 (Sw/SD/3/2005), was isolated from pigs with respiratory disease on a farm in eastern China. Genetic analysis revealed that Sw/SD/3/2005 was a triple-reassortant virus with a PB2 gene from human-like H1N1, NS from classical swine H1N1, and the remaining genes from human-like H3N2 virus. These findings further support the concept that swine can serve as reservoir or mixing vessels of influenza virus strains and maintain genetic and antigenic stability of viruses. Furthermore, we have successfully established a reverse genetics system based on eight plasmids and rescued Sw/SD/3/2005 through cell transfection. HI tests and RT-PCR confirmed that the rescued virus maintained the biological properties of the wild type Sw/SD/3/2005. The successful establishment of the reverse genetics system of Sw/SD/3/2005 will enable us to conduct extensive studies of the molecular evolution of H3N2 influenza viruses in swine. | Xian QI Yong-jun JIAO Hao PAN Lun-biao CUI Wei-xing FAN Bao-xu HUANG Zhi-yang SHI Hua WANG | 2009 | Virologica Sinica2009，24，1： | 5 |
| 13 | Sustained high level transgene expression in mammalian cells mediated by the optimized piggyBac transposon system显示文摘Sustained,high level transgene expression in mammalian cells is desired in many cases for studying gene functions.Traditionally,stable transgene expression has been accomplished by using retroviral or lentiviral vectors.However,such viral vector-mediated transgene expression is often at low levels and can be reduced over time due to low copy numbers and/or chromatin remodeling repression.The piggyBac transposon has emerged as a promising nonviral vector system for efficient gene transfer into mammalian cells.Despite its inherent advantages over lentiviral and retroviral systems,piggyBac system has not been widely used,at least in part due to their limited manipulation flexibilities.Here,we seek to optimize piggyBac-mediated transgene expression and generate a more efficient,user-friendly piggyBac system.By engineering a panel of versatile piggyBac vectors and constructing recombinant adenoviruses expressing piggyBac transposase(PBase),we demonstrate that adenovirusmediated PBase expression significantly enhances the integration efficiency and expression level of transgenes in mesenchymal stem cells and osteosarcoma cells,compared to that obtained from co-transfection of the CMV-PBase plasmid.We further determine the drug selection timeline to achieve optimal stable transgene expression.Moreover,we demonstrate that the transgene copy number of piggyBac-mediated integration is approximately 10 times higher than that mediated by retroviral vectors.Using the engineered tandem expression vector,we show that three transgenes can be simultaneously expressed in a single vector with high efficiency.Thus,these results strongly suggest that the optimized piggyBac system is a valuable tool for making stable cell lines with sustained,high transgene expression. | Xiang Chen Jing Cui Zhengjian Yan Hongmei Zhang Xian Chen Ning Wang Palak Shah Fang Deng Chen Zhao Nisha Geng Melissa Li Sahitya K.Denduluri Rex C.Haydon Hue H.Luu Russell R.Reid Tong-Chuan He | 2015 | Genes & Diseases2015，2，1： | 5 |
| 14 | Detection of Atrazine Residue in Food Samples by a Monoclonal Antibody-based Enzyme-linked Immunosorbent Assay显示文摘Atrazine(AT,2-chloro-4-ethylamino-6-isopropyl-amino-s-triazine)has been detected in ground water in several areas of the United States for many years,as well as in China,wherein the growth rate of its | LV Zhi Qiang WANG Cai Hong WANG Ting Ting CHEN Cui Cui WANG Ying NING Bao An LIU Ming LIU Jian Qing BAI Jia Lei PENG Yuan GAO Zhi Xian | 2013 | Biomedical and Environmental Sciences2013，26，5： | 5 |
| 15 | Discovery of differential sequences for improving breeding and yield of cultivated Ophiocordyceps sinensis through ITS sequencing and phylogenetic analysis显示文摘To accelerate the breeding process of cultivated Ophiocordyceps sinensis and increase its yield,it is important to identify molecular fingerprint of dominant O.sinensis.In the present study,we collected 3 batches of industrially cultivated O.sinensis product with higher yield than the others and compared their internal transcribed spacer(ITS)sequences with the wild and the reported.The ITS sequence was obtained by bidirectional sequencing and analyzed with molecular systematics as a DNA barcode for rapid and accurate identification of wild and cultivated O.sinensis collected.The ITS sequences of O.sinensis with detailed collection loci on NCBI were downloaded to construct a phylogenetic tree together with the sequences obtained from the present study by using neighbor-joining method based on their evolution relationship.The information on collection loci was analyzed with ArcGIS 10.2 to demonstrate the geographic distribution of these samples and thus to determine the origin of the dominant samples.The results showed that all wild and cultivated samples were identified as O.sinensis and all sequences were divided into seven phylogenetic groups in the tree.Those groups were precisely distributed on the map and the process of their system evolution was clearly presented.The three cultivated samples were clustered into two dominant groups,showing the correlation between the industrially cultivated samples and the dominant wild samples,which can provide references for its optimized breeding in the future. | CHENG Qi-Qing CHENG Chun-Song OUYANG Yue LAO Chi-Chou CUI Hao XIAN Yu JIANG Zhi-Hong LI Wen-Jia ZHOU Hua | 2018 | Chinese Journal of Natural Medicines2018，16，10： | 4 |
| 16 | Severe human infection with a novel avian-origin influenza A(H7N4) virus显示文摘Human infections with influenza H7 subtypes, such as H7N9, have raised concerns worldwide. Here, we report a human infection with a novel influenza A(H7N4) virus. A 68 years-old woman with cardiovascular and cholecystic comorbidities developed rapidly progressed pneumonia with influenza-like-illness as initial symptom, recovered after 23 days-hospitalization including 8 days in ICU. Laboratory indicators for liver and blood coagulation dysfunction were observed. Oseltamivir phosphate, glucocorticoids and antibiotics were jointly implemented, with nasal catheterization of oxygen inhalation for this patient.We obtained the medical records and collected serial respiratory and blood specimens from her. We collected throat, cloacal and/or feces samples of poultry and wild birds from the patient's backyard, neighborhood, local live poultry markets(LPMs) and the nearest lake. All close contacts of the patient were followed up and sampled with throat swabs and sera. Influenza viruses and other respiratory pathogens were tested by real-time RT-PCR, viral culturing and/or sequencing for human respiratory and bird samples. Micro-neutralizing assay was performed for sera. A novel reassortant wild bird-origin H7N4 virus is identified from the patient and her backyard poultry(chickens and ducks) by sequencing, which is distinct from previously-reported avian H7N4 and H7N9 viruses. At least four folds increase of neutralizing antibodies to H7N4 was detected in her convalescent sera. No samples from close contacts, wild birds or other poultry were tested positive for H7N4 by real-time RT-PCR. | Xiang Huo Lun-biao Cui Cong Chen Dayan Wang Xian Qi Ming-hao Zhou Xiling Guo Fengming Wang William J. Liu Weirong Kong Daxin Ni Ying Chi Yiyue Ge Haodi Huang Feifei Hu Chao Li Xiang Zhao Ruiqi Ren Chang-jun Bao George F. Gao Feng-Cai Zhu | 2018 | Science Bulletin2018，63，16： | 4 |
| 17 | Investigation of the deterioration of passive films in H_2S-containing solutions显示文摘The effect of H_2S on the corrosion behavior of 316 L stainless steel was investigated using electrochemical methods by changing the gas condition from CO_2 to H_2S and then back to CO_2. The presence of H_2S showed an acceleration effect on the corrosion of 316 L stainless steel in comparison with CO_2. The acceleration effect remained even after the complete removal of H_2S by CO_2, indicating that the passive film was irreversibly damaged. X-ray photoelectron spectroscopy(XPS) analysis indicated that the passive film was composed of Cr_2O_3, Fe_2O_3, and FeS_2 after being immersed in H_2S-containing solutions. The semiconducting property of the passive film was then investigated by using the Mott–Schottky approach. The presence of sulfides resulted in higher acceptor and donor densities and thus was responsible for the deterioration of passive films. | Zhu Wang Lei Zhang Xian Tang Zhao-yang Cui Jun-peng Xue Min-xu Lu | 2017 | International Journal of Minerals,Metallurgy and Materials2017，24，8： | 4 |
| 18 | A salt-induced viscosifying smart polymer for fracturing inter-salt shale oil reservoirs显示文摘Inter-salt shale oil reservoirs located between two salt layers are always accompanied by high temperature and high salinity. However, the present commonly used water-soluble polymers in fracturing fluids su er from poor tolerance to high temperature and high salinity. Thermoviscosifying polymers(TVP) whose aqueous solution shows viscosity increase upon increasing temperature and salt concentration have received considerable attention recently, which is promising for utilization in fracturing fluids to overcome these problems. In this work, both the salt-induced viscosifying property and mechanism of a TVP solution were investigated and the performance of TVP used as fracturing fluid based on the conditions of the Jianghan inter-salt shale oil reservoir in China was evaluated. It is found that the salt-induced viscosifying property of the TVP solution decreases with temperature and shear rate, but increases with polymer concentration. The number of intermolecular hydrophobic domains increases with the salt concentration contributing to the strengthening of a 3D network structure, which results in an increase in viscosity. In addition, the TVP fracturing fluid formulated with saturated brine exhibits excellent temperature and shear resistance, sand-suspending performance, and gel-breaking performance. Its viscosity remains above50 m Pa s after being sheared for 1 h even at a high temperature of 140 °C and the sand-suspending stability can be maintained for more than 1 week at 100 °C. Furthermore, the fracturing fluid can be easily broken down within 12h using 0.2 wt%–0.3 wt% potassium persulfate without residue. | Xian’e Li Hong-Yao Yin Ru-Sheng Zhang Jia Cui Jun-Wen Wu Yu-Jun Feng | 2019 | Petroleum Science2019，16，4： | 4 |
| 19 | Annexin A2 promotion of hepatocellular carcinoma tumorigenesis via the immune microenvironment显示文摘Hepatocellular carcinoma(HCC) is the most common primary liver cancer with a dismal prognosis, especially when diagnosed at advanced stages. Annexin A2(ANXA2), is found to promote cancer progression and therapeutic resistance.However, the underlining mechanisms of ANXA2 in immune escape of HCC remain poorly understood up to now. Herein, we summarized the molecular function of ANXA2 in HCC and its relationship with prognosis. Furthermore, we tentatively elucidated the underlying mechanism of ANXA2 immune escape of HCC by upregulating the proportion of regulatory T cells and the expression of several inhibitory molecules, and by downregulating the proportion of natural killer cells and dendritic cells and the expression of several inhibitory molecules or effector molecules. We expect a lot of in-depth studies to further reveal the underlying mechanism of ANXA2 in immune escape of HCC in the future. | Li-Wei Qiu Yi-Fei Liu Xiao-Qing Cao Yan Wang Xiao-Hong Cui Xian Ye Shuo-Wen Huang Hong-Jun Xie Hai-Jian Zhang | 2020 | World Journal of Gastroenterology2020，26，18： | 4 |
| 20 | Uptake of oxidative stress-mediated extracellular vesicles by vascular endothelial cells under low magnitude shear stress显示文摘Extracellular vesicles(EVs)are increasingly used as delivery vehicles for drugs and bioactive molecules,which usually require intravascular administration.The endothelial cells covering the inner surface of blood vessels are susceptible to the shear stress of blood flow.Few studies demonstrate the interplay of red blood cell-derived EVs(RBCEVs)and endothelial cells.Thus,the phagocytosis of EVs by vascular endothelial cells during blood flow needs to be elucidated.In this study,red blood cell-derived extracellular vesicles(RBCEVs)were constructed to investigate endothelial cell phagocytosis in vitro and animal models.Results showed that low magnitude shear stress including low shear stress(LSS)and oscillatory shear stress(OSS)could promote the uptake of RBCEVs by endothelial cells in vitro.In addition,in zebrafish and mouse models,RBCEVs tend to be internalized by endothelial cells under LSS or OSS.Moreover,RBCEVs are easily engulfed by endothelial cells in atherosclerotic plaques exposed to LSS or OSS.In terms of mechanism,oxidative stress induced by LSS is part of the reason for the increased uptake of endothelial cells.Overall,this study shows that vascular endothelial cells can easily engulf EVs in areas of low magnitude shear stress,which will provide a theoretical basis for the development and utilization of EVs-based nano-drug delivery systems in vivo. | Xian Qin Kun Zhang Juhui Qiu Nan Wang Kai Qu Yuliang Cui Junli Huang Li Luo Yuan Zhong Tian Tian Wei Wu Yi Wang Guixue Wang | 2022 | Bioactive Materials2022，7，3： | 3 |
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