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| 1 | Relevance of α-defensins(HNP1-3) and defensin β-1 in diabetes显示文摘AIM: To investigate the genetic background of human defensin expression in type 1 and 2 diabetes.METHODS: Associations between DEFA1/DEFA3 gene copy number polymorphism and diabetes as well as between the promoter polymorphisms of DEFB1 and diabetes were studied. The copy number variation of the DEFA1/DEFA3 genes was determined in 257 diabetic patients(117 patients with type 1 and 140 with type 2 diabetes). The control group consisted of 221 age- and gender-matched healthy blood donors. The cumulative copy numbers of the DEFA1/DEFA3 genes were detected by using quantitative PCR analysis. To evaluate the HNP 1-3(human neutrophil peptide 1-3 or α-defensin) levels in the circulation, plasma HNP 1-3 concentrations were measured by ELISA. The expression of DEFA1/A3 in peripheral leukocytes of the diabetic patients was measured by quantitative RT PCR analysis. Three SNPs of the human DEFB1(human defensin β-1) gene: DEFB1 G-20A(rs11362), DEFB1 C-44G(rs1800972) and DEFB1 G-52A(rs1799946) were genotyped by Custom TaqMan? Real Time PCR assay.RESULTS: Significant differences were observed in HNP1-3 levels between the healthy subjects and both groups of diabetic patients. The mean ± SE was 28.78 ± 4.2 ng/mL in type 1 diabetes, and 29.82 ± 5.36 ng/mL in type 2 diabetes, vs 11.94 ± 2.96 ng/mL in controls; P < 0.01 respectively. There was no significant difference between patients with type 1 and type 2 diabetes in the high plasma concentrations of HNP1-3. The highest concentrations of α-defensin were found in diabetic patients with nephropathy(49.4 ± 4.8 ng/mL), neuropathy(38.7 ± 4.8 ng/mL) or cardiovascular complications(45.6 ± 1.45 ng/L). There was no significant difference in the cumulative copy numbers of DEFA1/DEFA3 genes between controls and patients, or between patients with the two types of diabetes. Comparisons of HNP 1-3 plasma level and DEFA1/A3 copy number of the same patient did not reveal significant relationship between defensin-α levels and the gene copy numbers(r2 = 0.01). Similarly, no positive correlation was observed between the copy numbers and the mRNA expression levels of DEFA1/A3. Regarding the C-44G polymorphism of DEFB1, the GG 'protective' genotype was much less frequent(1%-2%) among both groups of patients than among controls(9%).CONCLUSION: Elevated HNP1-3 levels in diabetes are independent of DEFA1/DEFA3 copy numbers, but GG genotype of C-44G SNP in DEFB1 gene may result in decreased defensin β-1 production. | Balázs Csaba Németh Tamás Várkonyi Ferenc Somogyvári Csaba Lengyel Katalin Fehértemplomi Szabolcs Nyiraty Péter Kempler Yvette Mándi | 2014 | World Journal of Gastroenterology2014，20，27： | 4 |
| 2 | Increased duodenal expression of mi R-146a and-155 in pediatric Crohn's disease显示文摘AIM: To evaluate the role of micro RNA(mi R)-146 a,-155 and-122 in the duodenal mucosa of pediatric patients with Crohn's disease(CD) and the effect of transforming growth factor-β(TGF-β) on these mi Rs in duodenal epithelial and fibroblast cells.METHODS: Formalin-fixed, paraffin-embedded biopsies derived from the macroscopically inflamed(CD inflamed: n = 10) and intact(CD intact: n = 10) duodenal mucosa of pediatric CD patients and control children(C: n = 10) were examined. Expression of mi R-146 a,-155 and-122 was determined by realtime polymerase-chain reaction(PCR). The expression of the above mi Rs was investigated in recombinant human TGF-β(1 nmol/L, 24 h) or vehicle treated small intestinal epithelial cells(CCL-241) and primary duodenal fibroblast cells derived from healthy children as well.RESULTS: Expression of mi R-146 a was significantly higher in the inflamed duodenal mucosa compared to the intact duodenal mucosa of children with CD(CD inflamed: 3.21 ± 0.50 vs CD intact: 0.62 ± 0.26, p ≤ 0.01) and to the control group(CD inflamed: 3.21 ± 0.50 vs C: 1.00 ± 0.33, p ≤ 0.05). The expression of mi R-155 was significantly increased in the inflamed region of the duodenum compared to the control group(CD inflamed: 4.87 ± 1.02 vs Control: 1.00 ± 0.40, p ≤ 0.001). The expression of mi R-122 was unchanged in the inflamed or intact mucosa of CD patients compared to controls. TGF-β treatment significantly decreased the expression of mi R-155 in small intestinal epithelial cells(TGF-β: 0.7 ± 0.083 vs Control: 1 ± 0.09, p ≤ 0.05) and also the expression of mi R-146a(TGF-β: 0.67 ± 0.04 vs Control: 1 ± 0.15, p ≤ 0.01) and mi R-155(TGF-β: 0.72 ± 0.09 vs Control: 1 ± 0.06, p ≤ 0.05) in primary duodenal fibroblasts compared to corresponding vehicle treated controls. TGF-β treatment did not influence the expression of mi R-122.CONCLUSION: The elevated expression of mi R-146 a and-155 in the inflamed duodenal mucosa of CD patients suggests the role of these mi Rs in the pathomechanism of inflammatory bowel disease. Antiinflammatory TGF-β plays an important role in the regulation of the expression of these mi Rs. | Dániel Szucs Nóra Judit Béres Réka Rokonay Kriszta Boros Katalin Borka Zoltán Kiss András Arató Attila J Szabó ádám Vannay Erna Sziksz Csaba Bereczki Gábor Veres | 2016 | World Journal of Gastroenterology2016，22，26： | 2 |
| 3 | The hyperbolic tangent distribution family 显示文摘 | TIBOR B BELA L CSABA M | 1998 | Powder Technology1998，97，： | 2 |
| 4 | lonically crosslinked chitosan/tripolyphosphate nanoparticles for oligonucleotide and plasmid DNA delivery 显示文摘 | Csaba N Koping-Hoggard M Alonso MJ | 2009 | lnt J Pharm2009，382，1： | 1 |
| 5 | Capsaicin-sensitive local sensory innervation is involved in pacing-induced preconditioning in rat hearts: role of nitric oxide and CGRP?显示文摘 | P. Ferdinandy Tamás Csont Csaba Csonka Marianna T?r?k Mária Dux József Németh László I. Horváth László Dux Zoltán Szilvássy Gábor Jancsó | 1997 | Naunyn - Schmiedeberg’s Archives of Pharmacology1997，，3： | 1 |
| 6 | Effect of sleep on in-terictal spikes and distribution of sleep spindles on electrocor-ticography in children with focal epilepsy显示文摘 | Asano E Temeuzhka M Csaba J | 2007 | clinical Neuro-physiology2007，118，： | 1 |
| 7 | Production of a defensin-like antifungal protein NFAP from Neosartorya fischeri in Pichia pastoris and its antifungal activity against filamentous fungal isolates from human infections显示文摘 | Máté Virágh Dóra V?r?s Zoltán Kele Laura Kovács ádám Fizil Gergely Lakatos Gergely Maróti Gyula Batta Csaba Vágv?lgyi László Galgóczy | 2014 | Protein Expression and Purification2014，，： | 1 |
| 8 | Ionically crosslinked chitosan/tripolyphosphate nanoparticles for oligonucleotide and plasmid DNA delivery显示文摘 | Csaba N Koping-Haggurd M Alonso M J | 2009 | Int J Pharm2009，382，12： | 1 |
| 9 | Light-dependent induction of proline biosynthesis by abscisic acid and salt stress is inhibited by brassinosteroid in Arabidopsis显示文摘 | Edit ábrahám Gábor Rigó Gy?ngyi Székely Réka Nagy Csaba Koncz László Szabados | 2003 | Plant Molecular Biology2003，，3： | 1 |
| 10 | PATHOPHYSIOLOGICAL ROLES OF PEROXYNITRITE IN CIRCULATORY SHOCK显示文摘 | Csaba Szabó Katalin Módis | 2010 | 2010 (7 Su)2010，，7： | 1 |
| 11 | The effect of exercise and nettle supplementation on oxidative stress markers in the rat brain显示文摘 | Anna Toldy Krisztián Stadler Mária Sasvári Judit Jakus Kyung J. Jung Hae Y. Chung István Berkes Csaba Nyakas Zsolt Radák | 2005 | Brain Research Bulletin2005，，6： | 1 |
| 12 | Use of Pichia pastoris for Production of Recombinant Cytokines显示文摘 | Kevin P M Csaba P Mark D M | 2001 | Interleukin Protocols2001，60，： | 1 |
| 13 | Anxitey as an independent cardiovascular risk显示文摘 | Csaba B M | 2006 | Neu- ropsychophamacol Hung2006，8，1： | 1 |
| 14 | The performance of nanocarriers for transmucosal drug deliver显示文摘 | Csaba N Garcia-Fuentes M Alonso MJ | 2006 | Expert Opin Drug Deliv2006，3，4： | 1 |
| 15 | Cholinergic excition of septohippocampal GABA but not choliergic neurons: implications for learning and memory 显示文摘 | WU M MARYA S CSABA L | 2000 | J Neuronscince2000，20，10： | 1 |
| 16 | Association of activated vitamin D treatment and mortality in chronic kidney disease显示文摘 | Csaba P Kovesdy M D Shahram A | 2008 | Arch Intern Med2008，168，4： | 1 |
| 17 | Introduction of the family Alloherpesviridae: the first molecular detection of herpesviruses of cyprinid fish in Hungary显示文摘 | Doszpoly A Benko M Csaba G | 2011 | Magyar Allatorvosok Lapja2011，133，3： | 1 |
| 18 | Vaccine delivery carriers: Insights and future perspectives 显示文摘 | Correia-Pinto J Csaba N Alonso M | 2013 | Int J Pharm2013，440，1： | 1 |
| 19 | Application of 16S,18S rDNA and COI sequences in the molecular systematics of the earthworm family Lumbricidae(Annelida:Oligochaeta)显示文摘 | Antonia GC Wink M Csaba C Pop VV | | 0，，： | 1 |
| 20 | Intratracheal poly (ADP) ribose synthetase inhibition ameliorates lung ischemia reperfusion injury显示文摘 | Alexander S Farivar Steven M Woolley Charles H Fraga Robert Thomas Andrew L Salzman Csaba Szabo Michael S Mulligan | 2004 | The Annals of Thoracic Surgery2004，，6： | 1 |
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