|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Transcriptional down regulation of hTERT and senescence induction in HepG2 cells by chelidonine显示文摘AIM: To investigate the potential effects of chelidonine, the main alkaloid of Chelidonium majus, on telomerase activity and its regulation in HepG2 cells. METHODS: Cytotoxicity of chelidonine for HepG2 cells was determined by neutral red assay. A modified polymerase chain reaction (PCR)-based telomerase repeat amplification protocol was used to estimate relative telomerase activity in chelidonine-treated cells in comparison with the untreated control cells. Relative expression level of the catalytic subunit of telomerase (hTERT) gene and P-glycoprotein (pgp) were estimated using semi-quantitative real-time reverse transcription-PCR (RT-PCR). Cell senescence in treated cells was demonstrated using a β-galactosidase test. RESULTS: Cytotoxicity of chelidonine in HepG2 cells was not dose-dependent and tended to reach plateau immediately after the living cells were reduced in number to slightly higher than 50%. However, 12 μmol/L concentration of chelidonine was considered as LD50, where the maximal attainable effects were realized. Real-time RT-PCR data showed that the expression of pgp increased three-fold in chelidonine treated HepG2 cells in comparison with the untreated controls. Morphologically, treated HepG2 cells showed apoptotic features after 24 h and a small fraction of cells appeared with single blister cell death. The relative expressionlevel of Bcl-2 dropped to less than 50% of control cells at a sub-apoptotic concentration of chelidonine and subsequently increased to higher than 120% at LD50. Telomerase activity was reduced considerably after administration of very low doses of chelidonine, whereas higher concentrations of chelidonine did not remarkably enhance the effect. Real-time RT-PCR experiments indicated a drastic decrease in expression level of hTERT subunit of telomerase under treatment with chelidonine. Repeated treatment of cells with very low doses of chelidonine caused a decline in growth rate by 4 wk and many of the cells appeared to be aged with large volume and dark staining in the β-galactosidase assay. CONCLUSION: Chelidonine reduces telomerase activity through down-regulation of hTERT expression. Senescence induction might not be directly caused by reducing telomerase activity as it occurs after a few population doublings. | Sakineh Kazemi Noureini Michael Wink | 2009 | World Journal of Gastroenterology2009，15，29： | 5 |
| 2 | Degradation of intestinal mRNA:A matter of treatment显示文摘AIM: To characterize the influence of location, species and treatment upon RNA degradation in tissue samples from the gastrointestinal tract. METHODS: The intestinal samples were stored in different medium for different times under varyingconditions: different species(human and rat), varying temperature(storage on crushed ice or room temperature), time point of dissection of the submucous-mucous layer from the smooth muscle(before or after storage), different rinsing methods(rinsing with Medium, PBS, RNALater or without rinsing at all) and different regions of the gut(proximal and distal small intestine, caecum, colon and rectum). The total RNA from different parts of the gut(rat: proximal and distal small intestine, caecum, colon and rectum, human: colon and rectum) and individual gut layers(muscle and submucosal/mucosal) was extracted. The quality of the RNA was assessed by micro capillary electrophoresis. The RNA quality was expressed by the RNA integrity number which is calculated from the relative height and area of the 18 S and 28 S RNA peaks. From rat distal small intestine q PCR was performed for neuronal and glial markers.RESULTS: RNA obtained from smooth muscle tissue is much longer stable than those from submucosal/mucosal tissue. At RT muscle RNA degrades after one day, on ice it is stable at least three days. Cleaning and separation of gut layers before storage and use of RNALater, maintains the stability of muscle RNA at RT for much longer periods. Different parts of the gut show varying degradation periods. RNA obtained from the submucosal/mucosal layer always showed a much worse amplification rate than RNA from muscle tissue. In general RNA harvested from rat tissue, either smooth muscle layer or submucosal/mucosal layer is much longer stable than RNA from human gut tissue, and RNA obtained from smooth muscle tissue shows an increased stability compared to RNA from submucosal/mucosal tissue. At RT muscle RNA degrades after one day, while the stability on ice lasts at least three days. Cleaning and separation of gut layers before storage and use of RNALater, maintains the stability of muscle RNA at RT for much longer periods. Different parts of the gut show varying degradation periods. The RNA from muscle and submucosal/mucosal tissue of the proximal small intestine degrades much faster than the RNA of distal small intestine, caecum or colonwith rectum. RNA obtained from the submucosal/mucosal layer always showed a much more reduced amplification rate than RNA from muscle tissue [β-Tubulin Ⅲ for muscle quantification cycle(Cp): 22.07 ± 0.25, for β-Tubulin Ⅲ submucosal/mucosal Cp: 27.42 ± 0.19].CONCLUSION: Degradation of intestinal m RNA depends on preparation and storage conditions of the tissue. Cooling, rinsing and separating of intestinal tissue reduce the degradation of m RNA. | Sabine Heumüller-Klug Carsten Sticht Karin Kaiser Elvira Wink Cornelia Hagl Lucas Wessel Karl-Herbert Schafer | 2015 | World Journal of Gastroenterology2015，21，12： | 2 |
| 3 | Carbon-13:naturally occurring nucleoside 显示文摘 | Alan J J Winkely M W Grant D M | 1970 | Proc Nat Acad Sci1970，65，1： | 2 |
| 4 | 幽门螺杆菌感染的治疗显示文摘 | Wink A de Boer Guido N J Tytgat 游晓萍 姚炜 | 2001 | 英国医学杂志中文版2001，4，3： | 2 |
| 5 | Treatment of Pain from Osteoporotic Vertebral Collapse by Percutaneous PMMA Vertebroplasty显示文摘 | Winking M Stahk J P Oertel M | 2004 | Acta Neurochir2004，146，5： | 1 |
| 6 | The multifaceted roles of nitric oxide in cancer显示文摘 | Wink DA Vodovotz Y Laval J | 1998 | Carcinologenesis1998，19，： | 1 |
| 7 | Anthraquinones in Rheum palmatum and Rumex dentatus (Polygonaceae),and phorbol esters in Jatropha curcas (Euphorbiaceae) with molluscicidal activity against the schistosome vector snails Oncomelania,Biomphalaria and Bulinus显示文摘 | LIU S Y SPORER F WINK M JOURDANE J HENNING R Li Y L RUPPEL A | 1997 | Trop Med Int Health1997，2，2： | 1 |
| 8 | Treatment of pain from osteoporotic vertebral collapse by percutaneous PMMA vertebroplasty显示文摘 | M. Winking J. P. Stahl M. Oertel R. Schnettler D. K. B?ker | 2004 | Acta Neurochirurgica2004，，5： | 1 |
| 9 | Treatment of pain from osteoporotic vertebral collapse by percutaneous PMMA vertebroplasty 显示文摘 | Winking M Stahl JP Oertel M | 2004 | Acta Neurochir (Wien)2004，146，5： | 1 |
| 10 | Optimizing prostate cancer detection: 8 versus 12-core biopsy protocol显示文摘 | de la Rosette JJ Wink MH Mamonlakis C | 2009 | J Urol2009，182，： | 1 |
| 11 | The effect of various nitrie oxide - donor agents on hydrogen peroxide - mediated toxicity : A direct correlalion belween nitric oxide formation and protection 显示文摘 | Wink DA Cook JA Paeelli R | 1996 | Areh Bioehem Biophys1996，331，： | 1 |
| 12 | Two faces of narcissism显示文摘 | Wink P | 1991 | Journal of Personality and So- cial Psychology1991，61，4： | 1 |
| 13 | Randomized study of the safety and clinical utility of rotational angiography versus stand-ard angiography in the diagnosis of coronary artery disease显示文摘 | Maddux JT Wink O Messenger JC | | 0，，： | 1 |
| 14 | From agglomerations to technology -and knowledge driven clusters: Aeronautics cluster policies in Europe显示文摘 | Benzler G Wink R | 2010 | International Journal of Technol- ogy Management2010，50，34： | 1 |
| 15 | The mandibular deformity in hemifacial microsomia:a reassessment of the Pruzansky and Kaban classification 显示文摘 | WINK J D GOLDSTEIN J A THOMAS PALIGA J | 2014 | Plast Reconstr Surg2014，133，2： | 1 |
| 16 | Optimization of multi-slice helical respiration-correlated CT:the effects of table speed and rotation time显示文摘 | Wink NM McNitt-Gray MF | 2005 | Phys Med Biol2005，50，： | 1 |
| 17 | Production and secretion of recom- binant thaumatin in tobacco hairy root cultures显示文摘 | Pham N B Schafer H and Wink M | 2012 | Biotechnology Jour- nal2012，7，4： | 1 |
| 18 | The emissions and environmental impact of PM~0 and trace elements from a modem coal- fired power plant equipped with ESP and wet FGD 显示文摘 | Meij R Winke B 1 | 2004 | FuelProcess Technology2004，85，6： | 1 |
| 19 | Storage of Quinolizidine Alkaloids in Macrosiphum albifrons and Aphis genistae ( Homoptera : Aphididae ) 显示文摘 | WINK M WITYE L | 1991 | Entomologia Generalis1991，15，4： | 1 |
| 20 | Performance assessment of solution methods for load distribution problem of gear teeth 显示文摘 | Wink C H Serpa A L | 2008 | Mech Mach Theory2008，43，1： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员