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| 1 | Identify lymphatic metastasis-associated genes in mouse hepatocarcinoma cell lines using gene chip显示文摘AIM: In order to obtain lymphogenous metastasisassociated genes, we compared the transcriptional profiles of mouse hepatocarcinoma cell lines Hca-F with highly lymphatic metastasis potential and Hca-P with low lymphatic metastasis potential.METHODS: Total RNA was isolated from Hca-F and Hca-P cells and synthesized into double-stranded cDNA. In vitro transcription double-stranded cDNA was labeled with biotin (i.e. biotin-labeled cRNA, used as the probe). The cRNA probes hybridized with Affymetrix GeneChip() MOE430A (containing 22 690 transcripts, including 14 500 known mouse genes and 4 371 ESTs) respectively and the signals were scanned by the GeneArray Scanner. The results were then analyzed by bioinformatics.RESULTS: Out of the 14 500 known genes investigated,110 (0.8%) were up regulated at least 23 fold. Among the total 4 371 ESTs, 17 ESTs (0.4%) (data were not presented) were up regulated at least 23 fold. According to the Gene Ontology and TreeView analysis, the 110genes were further classified into two groups: differential biological process profile and molecular function profile.CONCLUSION: Using high-throughput gene chip method,a large number of genes and their cellular functions about angiogenesis, cell adhesion, signal transduction, cell motility, transport, microtubule-based process, cytoskeleton organization and biogenesis, cell cycle, transcription,chaperone activity, motor activity, protein kinase activity,receptor binding and protein binding might be involved in the process of lymphatic metastasis and deserve to be used as potential candidates for further investigation.Cyclin D1, Fosl1, Hsp47, EGFR and AR, and Cav-1 are selected as the possible candidate genes of the metastatic phenotype, which need to be validated in later experiments.ESTs (data were not presented) might indicate novel genes associated with lymphatic metastasis. Validating the function of these genes is helpful to identify the key or candidate gene/pathway responsible for lymphatic metastasis, which might be used as the diagnostic markers and the therapeutic targets for lymphatic metastasis. | BoSong Jian-WuTang BoWang Xiao-NanCui LiHou LuSun Li-MinMao Chun-HuiZhou YueDu Li-HuiWang Hua-XinWang Ren-ShuZheng LeiSun | 2005 | World Journal of Gastroenterology2005，11，10： | 19 |
| 2 | Synthesis of Nanosized NaY Zeolite by Confined Space Method显示文摘Nanosized NaY crystals have been prepared from metakaolin and sodium silicate by confined space synthesis with starch additive. It is found that the product has a narrow crystal size distribution (50-100 nm), high Si/Al ratio (Si/Al=4.6-6.1), high surface area (1090 m2/g) and the average diameter of nanosized NaY (75 nm) synthesized is 30 nm, it is smaller than that of without starch additive. | BoWANG HongZhuMA 等 | 2002 | Chinese Chemical Letters2002，13，4： | 18 |
| 3 | Construction and selection of subtracted cDNA library of mouse hepatocarcinoma cell lines with different lymphatic metastasis potential显示文摘AIM: In order to elucidate the molecular mechanism of lymphatic metastasis of hepatocarcinoma, we detected the difference of gene expression between mouse hepatocarcinoma cell lines Hca-F and Hca-P with different lymphatic metastasis potential.METHODS: cDNA of Hca-F cells was used as a tester and cDNA of Hca-P cells was used as a driver, cDNAs highly expressed in Hca-F cells were isolated by the suppression subtractive hybridization (SSH) method. The isolated cDNA was cloned into T/A cloning vector. The ligation products were transformed into DH5 α competent cells. Individual dories were randomly selected and used for PCR amplification.Vector DNA from positive clones was isolated for sequencing.RESULTS: There were 800 positive clones in amplified subtracted cDNA library. Random analysis of 160 clones with PCR showed that 95% of the clones contained 100-700 bp inserts. Analysis of 20 sequenced cDNA clones randomly picked from the SSH library revealed 4 known genes(mouse heat shock protein 84 ku, DNA helicase, ribosomal protein S13 ,ethanol induced 6 gene) and 3 expressed sequence tags (ESTs). Four cDNAs showed no homology and presumably represent novel genes.CONCLUSION: A subtracted cDNA library of differentially expressed genes in mouse heptocarcinoma cell lines with different lymphatic metastasis potential was successfully constructed with SSH and T/A cloning techniques. The library is efficient and lays a solid foundation for searching new lymphatic metastasis related genes. The expression of mouse heat shock protein gene, DNA helicase and other 4 novel gene may be different between mouse heptocarcinoma cell lines with different lymphatic metastasis potential. | LiHou Jan-WuTang Xiao-NanCui BoWang BoSong LeiSun | 2004 | World Journal of Gastroenterology2004，10，16： | 9 |
| 4 | Global analysis of a dynamical model for transmission of tuberculosis with a general contact rate 显示文摘 | BOWANG S TEWA J J | 2010 | Communications in Nonlinear Science Numerical Simulation2010，15，11： | 1 |
| 5 | An experimental study of heat transfer in aluminum castings during water quenching显示文摘 | Bowang Xiao Qigui Wang Parag Jadhav Keyu Li | 2010 | Journal of Materials Processing Tech2010，，14： | 1 |
| 6 | Robust methodology for determination of heat transfer coefficient distribution in convection显示文摘 | Bowang Xiao Qigui Wang Gang Wang Richard D. Sisson Yiming Rong | 2010 | Applied Thermal Engineering2010，，17： | 1 |
| 7 | Effects of different resuscitation fluids on the rheologic behavior of red blood cells, blood viscosity and plasma viscosity in experimental hemorrhagic shock显示文摘 | Lian Zhao BoWang Guoxing You | 2009 | Resuscitation2009，80，： | 1 |
| 8 | A single copy of a virus‐derived transgene encoding hairpin RNA gives immunity to barley yellow dwarf virus显示文摘 | Ming‐BoWang David C.Abbott Peter M.Waterhouse | 2008 | Molecular Plant Pathology2008，，6： | 1 |
| 9 | Genome-wide investigation of gene–environment interactions in colorectal cancer显示文摘 | Sabine Siegert Jochen Hampe Clemens Schafmayer Witigo Sch?nfels Jan-Hendrik Egberts Asta F?rsti Bowang Chen Jesús Lascorz Kari Hemminki Andre Franke Michael Nothnagel Ute N?thlings Michael Krawczak | 2013 | Human Genetics2013，，2： | 1 |
| 10 | Research on the Transmission Paths of National Culture from the Perspective of the Belt and Road显示文摘The Belt and Road initiative has promoted the connotative development and export of Chinese culture.To ensure a good international display and going globally of Chinese culture,we could follow such paths as excavating the rich elements of traditional Chinese culture,shaping the fine works,creating culture brands with Chinese characteristics,developing new culture brands,opening up the cultural market,strengthening cultural dialogue,innovating cultural transmission paths by gathering the cultural forces at home and abroad,and enhancing the appeal of national culture so as to increase the closeness and recognition of people along the route. | Chen Bowang Su Xiaoli | 2019 | 学术界2019，，12： | 1 |
| 11 | Local origin of two vegetative compatibility groups of Fusarium oxysporum f. sp. vasinfectum in Australia显示文摘 | BoWang Curt L.Brubaker Brett A.Summerell Peter H.Thrall Jeremy J.Burdon | 2010 | Evolutionary Applications (鈥?)2010，，5： | 1 |
| 12 | Experimental investigation of residual stresses in water and air quenched aluminum alloy castings显示文摘 | Xiao Bowang Rong Yiming Li Keyu | 2011 | Experimental and Applied Mechanics2011，，6： | 1 |
| 13 | Clinicopathologic variables predicting tumor response to neoadjuvant chemotherapy in patients with locally advanced gastric cancer显示文摘 | Lin BoWang Rong YueTeng Zi NongJiang Wen XianHu Min JunDong Xiao MingYuan Wen JunChen MeiJin Jian GuoShen | 2012 | J Surg Oncol2012，，3： | 1 |
| 14 | Tribo- logicallnvestigation of CaF2 Nanocrystals as- GreaseAdditives显示文摘 | LiboWang BoWang XiaoboWang | 2007 | Tribology International2007，40，： | 1 |
| 15 | Interlocked and Interdigitated Architectures from Self‐Assembly of Long Flexible Ligands and Cadmium Salts显示文摘 | Xin‐LongWang ChaoQin En‐BoWang LinXu Zhong‐MinSu Chang‐WenHu | 2004 | Angewandte Chemie International Edition2004，，38： | 1 |
| 16 | MANAGEMENT OF SPONTANEOUS RUPTURE OF HEPATOCELLULAR CARCINOMA显示文摘 | BoWang YiLu Xu‐fengZhang LiangYú Cheng‐enPan ZhengWu | 2008 | ANZ Journal of Surgery2008，，6： | 1 |
| 17 | Familial association of colorectal adenocarcinoma with cancers at other sites显示文摘 | Kari Hemminki Bowang Chen | 2004 | European Journal of Cancer2004，40，16： | 1 |
| 18 | Synthesis and photoluminescence kinetics of Ce^(3+)-doped CsPbI3 QDs with near-unity PLQY显示文摘CsPbI_(3)perovskite quantum dots(QDs)have great potential in optoelectronic devices due to their suitable band-gaps,but low photoluminescence quantum yields(PLQYs)and poor phase stability seriously impede their practical application.This paper reports the synthesis of Ce^(3+)-doped CsPbI_(3)QDs by a hot injection method.In the presence of the dopant(Ce^(3+)),the highest PLQY of CsPbI_(3)QDs reached 99%,i.e.,near-unity PLQY,and the photoluminescence(PL)emission of CsPbI_(3)QDs could be well maintained compared to that of the undoped ones.The photoluminescence kinetics of Ce^(3+)-doped CsPbI_(3)QDs was investigated by the ultrafast transient absorption technologies,which exhibited that the Ce^(3+)not only increased the density of excitonic states close to the high energy excitonic states(HES),but also provided more emissive channels.Moreover,the radiative recombination rates calculated by the combination of PL lifetime and PLQY further illustrated the Pb2+vacancies were filled with Ce^(3+)ions so that the PL quenching of the CsPbI_(3)QDs could be effectively prevented.The theoretic analysis uncovered the mechanism of the high PLQY and stable PL emission of the Ce^(3+)-doped CsPbI_(3)QDs. | Bowang Shu Yajing Chang Jinhua Zhang Xiaopeng Cheng Dabin Yu | 2021 | Nano Research2021，14，10： | 1 |
| 19 | Synthesis and Characterization of a Novel Polymer Electrolyte for Lithium-ion Battery显示文摘A novel polymer electrolyte with the formula of Li2B4O7-PVA for lithium-ion battery was synthesized and its ion conductivity and mechanical properties were also tested. It is found that the conductivity of the prepared polymer electrolytes is higher than that of LiClO4/PEO or LiClO4/EC-DMC by two or three orders in magnitude and a large delocalized bond formed in Li2B4O7-PVA lead to transportation of Li ion easier, this electrolyte possesses high thermo-stability and can be used under 200C. | YanPingLiang HongZhuMA BoWANG | 2004 | Chinese Chemical Letters2004，15，2： | 0 |
| 20 | Sulfamic Acid as a Cost-effective Catalyst for Acetolysis of Cyclic Ethers显示文摘Sulfamic acid has been used as an efficient catalyst and green alternative for conventional acidic materials to promote the acetolysis reaction of THF to produce 1, 4-diacetoxybutane. This method is also applicable in the acetolysis of other cyclic ethers, such as methyl substituted THF and tetrahydropyran and 1,4-dioxane which is less reactivity. | WeiZhongGONG BoWANG YahLongGU LiangYAN LiMingYANG JiShuanSUO | 2005 | Chinese Chemical Letters2005，16，6： | 0 |
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