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| 1 | Identify lymphatic metastasis-associated genes in mouse hepatocarcinoma cell lines using gene chip显示文摘AIM: In order to obtain lymphogenous metastasisassociated genes, we compared the transcriptional profiles of mouse hepatocarcinoma cell lines Hca-F with highly lymphatic metastasis potential and Hca-P with low lymphatic metastasis potential.METHODS: Total RNA was isolated from Hca-F and Hca-P cells and synthesized into double-stranded cDNA. In vitro transcription double-stranded cDNA was labeled with biotin (i.e. biotin-labeled cRNA, used as the probe). The cRNA probes hybridized with Affymetrix GeneChip() MOE430A (containing 22 690 transcripts, including 14 500 known mouse genes and 4 371 ESTs) respectively and the signals were scanned by the GeneArray Scanner. The results were then analyzed by bioinformatics.RESULTS: Out of the 14 500 known genes investigated,110 (0.8%) were up regulated at least 23 fold. Among the total 4 371 ESTs, 17 ESTs (0.4%) (data were not presented) were up regulated at least 23 fold. According to the Gene Ontology and TreeView analysis, the 110genes were further classified into two groups: differential biological process profile and molecular function profile.CONCLUSION: Using high-throughput gene chip method,a large number of genes and their cellular functions about angiogenesis, cell adhesion, signal transduction, cell motility, transport, microtubule-based process, cytoskeleton organization and biogenesis, cell cycle, transcription,chaperone activity, motor activity, protein kinase activity,receptor binding and protein binding might be involved in the process of lymphatic metastasis and deserve to be used as potential candidates for further investigation.Cyclin D1, Fosl1, Hsp47, EGFR and AR, and Cav-1 are selected as the possible candidate genes of the metastatic phenotype, which need to be validated in later experiments.ESTs (data were not presented) might indicate novel genes associated with lymphatic metastasis. Validating the function of these genes is helpful to identify the key or candidate gene/pathway responsible for lymphatic metastasis, which might be used as the diagnostic markers and the therapeutic targets for lymphatic metastasis. | BoSong Jian-WuTang BoWang Xiao-NanCui LiHou LuSun Li-MinMao Chun-HuiZhou YueDu Li-HuiWang Hua-XinWang Ren-ShuZheng LeiSun | 2005 | World Journal of Gastroenterology2005，11，10： | 19 |
| 2 | 医学模型分类及世界研发商显示文摘医学模型架起医学理论通往临床实践的桥梁。基础解剖示教模型、局部功能性模型、计算机互动模型、虚拟培训系统、生理驱动模拟系统,这五部分各自具有不同功能,在医学生培养中缺一不可。生理驱动模拟系统再造“真实临床环境”,帮助医学生最终实现临床梦想。 | Academy and marketing Department of Rizheng Huarui Enterprise LuSun Wyatt(Ri Zheng Hua Rui) China Co.,Ltd., No.9, Fangzhuang Nanlu, Fengtai District, Beijing, 100079, PRC | 2007 | 中国医院2007，11，4： | 7 |
| 3 | 现代医学模拟实验室的建设、应用和管理显示文摘早在20世纪90年代,全英医学总会(GMC)就已经公布了医学教育的改革蓝图。呼吁通过设立医学理论知识、医患沟通技巧培养以及临床技能培训相结合的医学课程设置,减轻医学生的负担。同时,临床医学管理机构制定了医学保健从业人员的从业标准,这无疑对医学本科和研究生的培养模式产生了影响。针对上述各种情况,临床技能实验室、专科技能培训中心和医学模拟中心应运而生。他们成为医学院学生积累临床医学经验的场所。 | Academy and marketing Department of Rizheng Huarui Enterprise LuSun Wyatt(Ri Zheng Hua Rui) China Co.,Ltd., No.9, Fangzhuang Nanlu, Fengtai District, Beijing, 100079, PRC | 2007 | 中国医院2007，11，3： | 3 |
| 4 | 医学人才培养与现代医学模拟技术显示文摘时代在进步,医务人员自身医学伦理素养不断提高,病人自我保护意识不断增强,医学生临床实习日渐遥远。现代医学模拟技术再现真实临床环境,通过与医学理论知识有机结合、培训医学生及在职医务人员临床操作技能、全面培养职业医师素养,成为将医学生培养成及在职医务人员继续教育的必备教学、培训工具。 | LuSun Wyatt(Ri Zheng Hua Rui)China Co.,Ltd.,No.9,Fangzhuang Nanlu,Fengtai District,Beijing,100079,PRC | 2007 | 中国医院2007，11，2： | 1 |
| 5 | Spun highly linearly birefringent fibres for current sensors 显示文摘 | Qian Jingren Li Lusun | 1990 | Science in China(Series A)1990，33，1： | 1 |
| 6 | Body Mass Index (BMI), Waist to Hip Ratio (WHR) and Risk of Biliary Tract Cancers: A Population-based Case-control Study in Shanghai, China显示文摘OBJECTIVE To investigate the relationship between BMI, WHR and biliary tract cancers (CBT).METHODS A population-based case-control study was conducted in urban Shanghai from June 1, 1997 to May 31,2001 involving interviews with 627 new cases of biliary tract cancers aged 35 to 74 years and 959 frequency-matched population controls by gender and age in five-year groups. All subjects were interviewed in person by trained interviewers using a structured questionnaire. An unconditional logistic regression was performed to calculate adjusted odds ratios (ORs) and 95% confidence intervals (CIs).RESULTS Obesity was associated with an increased risk of gallbladder cancer across adulthood at ages 20-29 and 30-39 in females. Compared with subjects in the lowest quartile of WHR, ORs for the highest quartile and P for trend for cancers of gallbladder and extrahepatic bile duct both reached significant levels among males and females.CONCLUSION Our observations in urban Shanghai suggested that obesity in early adult life may contribute to the risk of gallbladder cancer, and increased WHR may substantially elevated risk of cancers of the gallbladder and extrahepatic bile duct. | XuehongZhang YutangGao AsifRashid JieDeng EnjuLiu KaiWu LuSun JiarongCheng GloriaGridley AnnWHsing | 2005 | Chinese Journal of Clinical Oncology2005，2，1： | 0 |
| 7 | Like Mother,Like Daughter显示文摘On the road to maturity, one's parents always have a great degree of influence. Yang Yuying, a young singer, attributes much of her success to her mother Xiong Yonghong. The hard working mother has exerted an imperceptible influence on her daughter's attitude to life with her traditional Chinese virtues. | LI LUSUN WEI LIANG | 2000 | Women of China2000，，3： | 0 |
| 8 | In vitro identification of metabolites of verapamil in rat liver microsomes显示文摘AIM: To investigate the metabolism of verapamil at low concentrations in rat liver microsomes. METHODS: Liver microsomes of Wistar rats were prepared using ultracentrifuge method. The in vitro metabolism of verapamil was studied with the rat liver microsomal incubation at concentration of 1.0 μmol/L and 5.0 μmol/L. The metabolites were separated and assayed by liquid chromatography-ion trap mass spectrometry (LC/MS^n), and further identified by comparison of their mass spectra and chromatographic behaviors with reference substances. RESULTS: Eightmetabolites, including two novel metabolites (M4 and MS), were found in rat liver microsomal incubates. They were identified as O-demethyl-verapamil isomers (M1 - M4), N-dealkylated derivatives of verapamil (MS-MT), and N, O-didemethyl-verapamil (MS). CONCLUSION: O-Demethylation and N-dealkylation were the main metabolic pathways of verapamil at low concentrations in rat liver microsomes, and the relative proportion of them in verapamil metabolism changed with different substrate concentrations. | LuSUN Shu-qiuZHANG Da-fangZHONG | 2004 | Acta Pharmacologica Sinica2004，25，1： | 0 |
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