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| 1 | Antioxidant efficiency of lycopene on oxidative stress-induced damage in bovine spermatozoa显示文摘Background: Lycopene(LYC) is a natural carotenoid with powerful reactive oxygen species(ROS) scavenging activities. The aim of this study was to investigate if lycopene has the ability to reverse ROS-mediated alterations to the motility, viability and intracellular antioxidant profile of bovine spermatozoa subjected to ferrous ascorbate(Fe AA). Spermatozoa were washed out of fresh bovine semen, suspended in 2.9 % sodium citrate and subjected to LYC treatment(0.25, 0.5, 1 or 2 mmol/L) in the presence or absence of Fe AA(150 μmol/L Fe SO_4 and 750 μmol/L ascorbic acid) during a 6 h in vitro culture. Spermatozoa motion characteristics were assessed using the Sperm Vision?computer-aided sperm analysis(CASA) system. Cell viability was examined with the metabolic activity(MTT) assay,ROS generation was quantified via luminometry and the nitroblue-tetrazolium(NBT) test was applied to quantify the intracellular superoxide formation. Cell lysates were prepared at the end of the in vitro culture to investigate the intracellular activity of superoxide dismutase(SOD), catalase(CAT), glutathione peroxidase(GPx) as well as the concentrations of glutathione(GSH) and malondialdehyde(MDA).Results: FeA A treatment led to a reduced spermatozoa motility(P < 0.001), viability(P < 0.001) and a decline of the antioxidant capacity of spermatozoa(P < 0.001) but increased the ROS generation(P < 0.001), superoxide production(P < 0.001) and lipid peroxidation(P < 0.001). LYC administration resulted in a preservation of the spermatozoa motion parameters(P < 0.001), mitochondrial activity(P < 0.001) and antioxidant characteristics(P < 0.001 with respect to SOD;P < 0.01 in relation to CAT; P < 0.05 as for GPx and GSH) with a concentration range of 1 and 2 mmol/L LYC revealed to be the most effective.Conclusions: Our results suggest that LYC exhibits significant ROS-scavenging and antioxidant properties which may prevent spermatozoa alterations caused by oxidative stress, and preserve the functionality of male reproductive cells. | Eva Tvrdá Anton Kovácik Eva Tusimová Dusan Paál Alica Mackovich Jakhongir Alimov Norbert Lukác | 2017 | Journal of Animal Science and Biotechnology2017，8，1： | 3 |
| 2 | Im+ provement of the stability of glucose oxidase via encapsulation in sodium alginate-ccllulose sulfate-poly (methyiene-co-guani- dine) capsules显示文摘 | Alica Vikartovskfi Marek Bfiko Danica Mislovieovfi | 2007 | Enzyme and Microbial Technology2007，41，6： | 1 |
| 3 | Can the Chinese Trade Surplus be Reduced through Exchange Rate Policy? 显示文摘 | Alica GarcIa-Herrero Tuuli Koivu | 2007 | BOFIT Discussion Paper2007，，： | 1 |
| 4 | High prevalence of metabolic syndrome in an elderly Croatian population – a multicentre study显示文摘 | Daria Pa?ali? Slavica Dodig Naima ?orovi? Alica Pizent Jasna Jurasovi? Mladen Pavlovi? | 2010 | Public Health Nutrition2010，，9： | 1 |
| 5 | The role of flagella and motility in the adherence and invasion to the fish cell lines by Aeromonas hydrophila显示文摘 | Susana M Xavier R Alica A | 1997 | FEMS Microbiology Letter1997，151，： | 1 |
| 6 | Improvement of the stability of glucose oxidase via encapsulation in sodium alginate–cellulose sulfate–poly(methylene- co -guanidine) capsules显示文摘 | Alica Vikartovská Marek Bu?ko Danica Mislovi?ová Vladimír P?toprsty Igor Lacík Peter Gemeiner | 2007 | Enzyme and Microbial Technology2007，，6： | 1 |
| 7 | Isolation of soluble collagen from Hake skin显示文摘 | Alica S C Maria E P Alicia N F | 1997 | Journal of Aquatic Food Product Technology1997，61，1： | 1 |
| 8 | Type and subtype specific detection of influenza viruses in clinical specimens by rapid culturv assay显示文摘 | Ziegler T Hall H Alica S F | 2002 | J Clin Microbio12002，33，： | 1 |
| 9 | Chelate-enhanced phytoremediation of soils polluted with heavy metals显示文摘 | ALKORTA I HERNANDEZ ALICA J BECERRIL JM | 2004 | Reviews in Environmental Science and Bio/Technology2004，，3： | 1 |
| 10 | Parameters of blood viscosity do not correlate with the extent of coronary and carotid atherosclerosis and with endothelial function in patients undergoing coronary angiography显示文摘 | B. Hiebl F. Jung Markus Vosseler Alica Beutel Sarina Sch? fer Selina Muxel Alexander Jabs Sandro Forconi John D. Parker Thomas Mü nzel Tommaso Gori | 2012 | 2012 (2-4)2012，，2： | 1 |
| 11 | Regulation of endothelial growth factor synthesis by nitric oxide: Facts and controversies 显示文摘 | Dulk J Alica J | 2003 | Antioxidants and redox signaling2003，5，1： | 1 |
| 12 | Behavior of cold-formed steel wall panels under monotonic horizontal loading显示文摘 | Eray Baran Cagatay Alica | 2012 | Journal of Constructional Steel Research2012，，： | 1 |
| 13 | Behavior of Cold-formed Steel Wall Panels under Monotonic Horizontal Loading 显示文摘 | Eray Baran Cagatay Alica | 2012 | Journal of Constructional Steel Research2012，79，： | 1 |
| 14 | The Cognitive Electrophysiology of Mind and Brain显示文摘 | Alberto Zani Alice Mado Proverbio Alica Mado Proverbio (Ed) | 2002 | Publisher: Academic Press2002，3341，： | 1 |
| 15 | Antibacterial activity against Clostridium genus and antiradical activity of the essential oils from different origin显示文摘 | Miroslava Ka?ániová Nenad Vukovi? Elena Horská Ivan ?alamon Alica Bobková Luká? Hleba Martina Fiskelová Alexander Vat?ák Jana Petrová Marek Bobko | 2014 | Journal of Environmental Science and Health Part B2014，，7： | 1 |
| 16 | Randomness of resting-state brain oscillations encodes Gray’s personality trait显示文摘 | Tim Hahn Thomas Dresler Ann-Christine Ehlis Martin Pyka Alica C. Dieler Claudia Saathoff Peter M. Jakob Klaus-Peter Lesch Andreas J. Fallgatter | 2011 | Neuroimage2011，，2： | 1 |
| 17 | Cow excrements enhance the occurrence of tetracycline resistance genes in soil regardless of their oxytetracycline content显示文摘 | Martina K Jiri J Alica C | 2013 | Chemosphere2013，93，10： | 1 |
| 18 | Isolation of solubie collagen form Hake skin显示文摘 | Alica S C Maria E P Alicia N F | 1997 | Journal of Aquatic Food Product Technology1997，61，1： | 1 |
| 19 | 将热爱变成事业——专访世界华人网球协会会长 方宗刚显示文摘“你的健康,我的快乐;网球联谊,全球华人”是世界华人网球协会不变的口号,热爱网球的方会长,享受网球带来的健康与快乐,也希望通过自己的努力将国外先进的网球理念与体制带到国内,促进中国网球的发展。 | Alica | 2016 | 网球大师2016，0，6： | 0 |
| 20 | Phylogenomic reconstruction addressing the Peltigeralean backbone(Lecanoromycetes,Ascomycota)显示文摘Rapid radiations in Fungi are only beginning to be studied with phylogenomic data.The evolutionary history of the lichenized fungal order Peltigerales has not been well resolved,particularly for the Collematineae.Here,we used concatenation and coalescent-based species tree methods to reconstruct the phylogeny of the Peltigerales based on sequences of 125 nuclear single-copy exon sequences among 60 samples,representing 58 species.Despite uneven,lineage-specific missing data and significant topological incongruence of individual exon trees,the resulting phylogenies were concordant and successfully resolved the phylogenetic relationships of the Peltigerales.Relationships in the Collematineae were defined by short branches and lower nodal support than in other parts of the tree,due in part to conflicting signal in exon trees,suggesting rapid diversification events in the early evolution of the suborder.Using tree distance measures,we were able to identify a minimum subset of exons that could reconstruct phylogenetic relationships in Peltigerales with higher support than the 125-exon dataset.Comparisons between the minimum and complete datasets in species tree inferences,bipartition analyses,and divergence time estimations displayed similar results,although the minimum dataset was characterized by higher levels of error in estimations of divergence times.Contrasting our inferences from the complete and minimum datasets to those derived from few nuclear and mitochondrial loci reveal that our topology is concordant with topologies reconstructed using the nuclear large subunit and mitochondrial small subunit ribosomal DNA markers,but the target capture datasets had much higher support values.We demonstrated how target capture approaches can effectively decipher ancient rapid radiations in cases where well resolved individual exon trees are sufficiently sampled and how to identify subsets of loci that are appropriate for fungal order-level phylogenetics. | Todd J.Widhelm Felix Grewe Bernard Goffinet Mats Wedin Trevor Goward Luis F.Coca Isabel Distefano Alica Košuthová· H.Thorsten Lumbsch | 2021 | Fungal Diversity2021，，5： | 0 |
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