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| 1 | Role of autophagy in differential sensitivity of hepatocarcinoma cells to sorafenib显示文摘AIM: To investigate the role of sorafenib(SFN) in autophagy of hepatocellular carcinoma(HCC). We evaluated how SFN affects autophagy signaling pathway in human HCC cell lines. METHODS: Two different human HCC cell lines, Hep3 B and Huh7, were subjected to different concentrations of SFN. Cell viability and onset of apoptosis were determined with colorimetric assay and immunoblotting analysis, respectively. The changes in autophagy-related proteins, including LC3, ULK1, AMPK, and LKB, were determined with immunoblotting analysis in the presence or absence of SFN. To assess autophagic dynamics, autophagic flux was measured with chloroquine, a lysosomal inhibitor. The autophagic responsiveness between different HCC cell lines was compared under the autophagy enhancing conditions.RESULTS: Hep3 B cells were significantly more resistant to SFN than Huh7 cells. Immunoblotting analysis revealed a marked increase in SFN-mediated autophagy flux in Huh7 cells, which was, however, absent in Hep3 B cells. While both starvation and rapamycin enhanced autophagy in Huh7 cells, only rapamycin increased autophagy in Hep3 B cells. Immunoblotting analysis of autophagy initiation proteins showed that SFN substantially increased phosphorylation of AMPK and consequently autophagy in Huh7, but not in Hep3 B cells.CONCLUSION: The autophagic responsiveness to SFN is distinct between Hep3 B and Huh7 cells. Resistance of Hep3 B cells to SFN may be associated with altered autophagy signaling pathways. | Trevan D Fischer Jin-Hee Wang Adrian Vlada Jae-Sung Kim Kevin E Behrns | 2014 | World Journal of Hepatology2014，6，10： | 5 |
| 2 | Diversity of endophytes across the soil-plant continuum for Atriplex spp. in arid environments显示文摘Endophytes are hypothesized to be transferred across the soil-plant continuum, suggesting both the transfers of endophytes from environment to plant and from plant to soil. To verify this hypothesis and to assess the role of locality, we evaluated the similarity of microbial communities commonly found both in soils and endophytic communities in three arid regions, i.e. the Jornada LTER(Long Term Ecological Research) site in New Mexico, USA, and the research station of Jordan University of Science and Technology(JUST) and Khanasri research station in Badia region of Jordan. Rhizosphere and non-rhizosphere soils, leaves and seeds of Atriplex spp. were sampled. Diversity and distribution of bacteria and fungi across the soil-plant continuums were assessed by tag-encoded FLX amplicon pyrosequencing and sequence alignment. Of the total bacterial OTUs(operational taxonomic units), 0.17% in Khanasri research station, 0.16% in research station of JUST, and 0.42% in Jornada LTER site were commonly found across all the plant and soil compartments. The same was true for fungi in two regions, i.e. 1.56% in research station of JUST and 0.86% in Jornada LTER site. However, in Khanasri research station, 12.08% of total fungi OTUs were found in at least one soil compartment and one plant compartment. Putative Arthrobacter, Sporosarcina, Cladosporium and members of Proteobacteria and Actinobacteria were found across all the soil-plant continuums. Ascomycota, mainly including Didymellaceae, Pleosporaceae and Davidiellaceae were present across all the soil-plant continuums. Microbial communities in two regions of Jordan were similar to each other, but both of them were different from the Jornada LTER site of USA. SIMPER(similarity percentage) analysis of bacterial and fungal taxa for both soil and endophyte communities revealed that dissimilarities of two bacterial genera(Arthrobacter and Sporosarcina) and two fungal genera(Cladosporium and Alternaria) are very high, so they play key roles in the soil-plant continuums. A weighed Pearson correlation analysis for the specific bacterial OTUs in the soil-plant continuums only showed high similarity between the two regions of Jordan. However, fungal groups showed higher similarities among all regions. This research supports the hypothesis of continuity of certain bacterial and fungal communities across the soil-plant continuums, and also explores the influences of plant species and geographic specificity on diversity and distribution of bacteria and fungi. | Mohammad E TAHTAMOUNI Sa'eb KHRESAT Mary LUCERO Jesus SIGALA Adrian UNC | 2016 | Journal of Arid Land2016，8，2： | 4 |
| 3 | Early infant male circumcision:Systematic review,risk-benefit analysis,and progress in policy显示文摘AIM To determine whether recent evidence-based United States polices on male circumcision(MC) apply to comparable Anglophone countries,Australia and New Zealand.METHODS Articles in 2005 through 2015 were retrieved from PubM ed using the keyword 'circumcision' together with 36 relevant subtopics.A further PubM ed search was performed for articles published in 2016.Searches of the EMBASE and Cochrane databases did not yield additional citable articles.Articles were assessed for quality and those rated 2+ and above according to the Scottish Intercollegiate Grading System were studied further.The most relevant andrepresentative of the topic were included.Bibliographies were examined to retrieve further key references.Randomized controlled trials,recent high quality systematic reviews or meta-analyses(level 1++ or 1+ evidence) were prioritized for inclusion.A risk-benefit analysis of articles rated for quality was performed.For efficiency and reliability,recent randomized controlled trials,metaanalyses,high quality systematic reviews and large welldesigned studies were used if available.Internet searches were conducted for other relevant information,including policies and Australian data on claims under Medicare for MC.RESULTS Evidence-based policy statements by the American Academy of Pediatrics(AAP) and the Centers for Disease Control and Prevention(CDC) support infant and later age male circumcision(MC) as a desirable public health measure.Our systematic review of relevant literature over the past decade yielded 140 journal articles that met our inclusion criteria.Together,these showed that early infant MC confers immediate and lifelong benefits by protecting against urinary tract infections having potential adverse long-term renal effects,phimosis that causes difficult and painful erections and 'ballooning' during urination,inflammatory skin conditions,inferior penile hygiene,candidiasis,various sexually transmissible infections in both sexes,genital ulcers,and penile,prostate and cervical cancer.Our risk-benefit analysis showed that benefits exceeded procedural risks,which are predominantly minor,by up to 200 to 1.We estimated that more than 1 in 2 uncircumcised males will experience an adverse foreskin-related medical condition over their lifetime.Wide-ranging evidence from surveys,physiological measurements,and the anatomical location of penile sensory receptors responsible for sexual sensation strongly and consistently suggested that MC has no detrimental effect on sexual function,sensitivity or pleasure.United States studies showed that early infant MC is cost saving.The evidence supporting early infant MC has further strengthened since the positive AAP and CDC reviews.CONCLUSION Affirmative MC policies are needed in Australia and New Zealand.Routine provision of accurate,unbiased education,and access in public hospitals,will maximize health and financial benefits. | Brian J Morris Sean E Kennedy Alex D Wodak Adrian Mindel David Golovsky Leslie Schrieber Eugenie R Lumbers David J Handelsman John B Ziegler | 2017 | World Journal of Clinical Pediatrics2017，6，1： | 2 |
| 4 | Effect of graft source on unrelated donor haemopoietic stem-cell transplantation in adults with acute leukaemia: a retrospective analysis显示文摘 | Mary Eapen Vanderson Rocha Guillermo Sanz Andromachi Scaradavou Mei-Jie Zhang William Arcese Anne Sirvent Richard E Champlin Nelson Chao Adrian P Gee Luis Isola Mary J Laughlin David I Marks Samir Nabhan Annalisa Ruggeri Robert Soiffer Mary M Horowitz Eli | 2010 | Lancet Oncology2010，，7： | 2 |
| 5 | Three-dimensional vortex organization in a high-Reynolds-number supersonic turbulent boundary layer显示文摘 | Elsinga G E Adrian R J Van Oudheusden B W | 2010 | J Fluid Mech2010，644，： | 1 |
| 6 | Theoretical com- parison of low GWP alternatives for different refrigeration configurations taking R404A as baseline显示文摘 | Adrian M B Joaquin N E Angel B | 2014 | International Journal of Refrigeration2014，44，1： | 1 |
| 7 | Bombesin receptor subtype mediation of gastroenteropancreatic hormone seere-tion in rats 显示文摘 | Varga G Adrian T E Coy D H | 1994 | Peptides1994，15，4： | 1 |
| 8 | CD28 costimulation improves expansion and persistence of chimeric antigen receptor-modified T cells in lymphoma patients显示文摘 | Savoldo Barbara Ramos Carlos Almeida Liu Enli Mims Martha P Keating Michael J Carrum George Kamble Rammurti T Bollard Catherine M Gee Adrian P Mei Zhuyong Liu Hao Grilley Bambi Rooney Cliona M Heslop Helen E Brenner Malcolm K Dotti Gianpie | 2011 | Journal of Clinical Investigation2011，，5： | 1 |
| 9 | Drop-in energy performance evaluation of R1234yf and R1234ze(E) in a vapor compression system as R134a replacements 显示文摘 | Adrian M B Joaquin N E Angel B | 2014 | Ap- plied Thermal Engineering2014，71，1： | 1 |
| 10 | Small-scale spatial soil-plant relationship in semi-arid gypsum environments显示文摘 | Agustin R Adrian E | 2000 | Plant and Soil2000，220，： | 1 |
| 11 | Maximally Maintained Inequality: Expansion,keform,and Opportunity in Irish Education,1921-1975显示文摘 | Adrian E·Raftery and Michael Hout | 1993 | Sociology of Education1993，，66： | 1 |
| 12 | A Two-Phase Cinematic PIV Method for Bubbly Flows 显示文摘 | Oakley T R Loth E Adrian R J | 1997 | Journal of Fluids Engineering1997，，119： | 1 |
| 13 | Tumor necrosis factor stimulates amylin secretion but inhibits insulin from islet β-cells by independent pathways显示文摘 | Ding X Z Flatt P R Adrian T E | 1996 | Regulatory Peptides1996，64，3： | 1 |
| 14 | Partitioning solute transport between infiltration and overland flow under rainfall显示文摘 | Havis R N Smith R E Adrian D D | 1992 | Water Resources Research1992，28，10： | 1 |
| 15 | Bayes factors显示文摘 | Robert E Raftery Adrian E | 1995 | Journal of the American Statistical Association1995，90，： | 1 |
| 16 | Implementation of a Lagrangian relaxation based unit commitment problem显示文摘 | Virmani S Adrian E C Imhof K | 1989 | IEEE Trans on Power Systems1989，4，4： | 1 |
| 17 | Strictly proper scoring rules, prediction and estimation 显示文摘 | TILMANN G ADRIAN E | 2007 | Journal of the American Statistical Association2007，102，477： | 1 |
| 18 | A new ap- proach in numerical assessment of the cavitation beha- viour of centrifugal pumps 显示文摘 | ADRIAN S ROMEO S R LIVIU E A | 2011 | International journal of fluid machinery and systems2011，4，1： | 1 |
| 19 | Incorporation of thymidine - H3 by cells in normal and injured mouse spinal cord 显示文摘 | Adrian E K Walker B E | 1962 | J Neuropathol Exp Neurol1962，21，： | 1 |
| 20 | All-atom structure prediction and folding simulations of a stable protein显示文摘 | Carlos Simmerling Bentley Strockbine Adrian E Roitberg | 2002 | J Am Chem Soc2002，124，11： | 1 |
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