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| 1 | Helicobacter pylori in dental plaque and stomach of patients from Northern Brazil显示文摘AIM: To establish whether virulence factor genes vacA and cagA are present in Helicobacter pylori (H. pylori) retrieved from gastric mucosa and dental plaque in pa-tients with dyspepsia. METHODS: Cumulative dental plaque specimens and gastric biopsies were submitted to histological exami-nation, rapid urease test and polymerase chain reac-tion (PCR) assays to detect the presence of cagA and vacA polymorphisms.RESULTS: Detection of H. pylori from dental plaque and gastric biopsy samples was greater by PCR com-pared to histological examination and the rapid ure-ase test. DNA from H. pylori was detected in 96% of gastric mucosa samples and in 72% of dental plaque samples. Sixty-three (89%) of 71 dental plaque sam-ples that were H. pylori-positive also exhibited identical vacA and cagA genotypes in gastric mucosa. The most common genotype was vacAs1bm1 and cagA positive, either in dental plaque or gastric mucosa. These viru-lent H. pylori isolates were involved in the severity of clinical outcome.CONCLUSION: These pathogenic strains were found simultaneously in dental plaque and gastric mucosa, which suggests that gastric infection is correlated with the presence of H. pylori in the mouth. | Mnica Baraúna Assumpo Luisa Caricio Martins Hivana Patricia Melo Barbosa Katarine Antonia dos Santos Barile Sintia Silva de Almeida Paulo Pimentel Assumpo Tereza Cristina de Oliveira Corvelo | 2010 | World Journal of Gastroenterology2010，16，24： | 29 |
| 2 | Promoter hypermethylation of CDH1, FHIT, MTAP and PLAGL1 in gastric adenocarcinoma in individuals from Northern Brazil显示文摘AIM: To evaluate the methylation status of CDH1, FHIT, MTAP and PLAGL1 promoters and the association of these findings with clinico-pathological characteristics. METHODS: Methylation-specific PCR (MSP) assay was performed in 13 nonneoplastic gastric adenocarcinoma, 30 intestinal-type gastric adenocarcinoma and 35 diffuse- type gastric adenocarcinoma samples from individuals in Northern Brazil. Statistical analyses were performed using the chi-square or Fisher’s exact test to assess associations between methylation status and clinico- pathological characteristics. RESULTS: Hypermethylation frequencies of CDH1, FHIT, MTAP and PLAGL1 promoter were 98.7%, 53.9%, 23.1% and 29.5%, respectively. Hypermethylation of three or four genes revealed a significant association with diffuse-type gastric cancer compared with nonneoplastic cancer. A higher hypermethylation frequency wassignificantly associated with H pylori infection in gastric cancers, especially with diffuse-type. Cancer samples without lymph node metastasis showed a higher FHIT hypermethylation frequency. MTAP hypermethylation was associated with H pylori in gastric cancer samples, as well as with diffuse-type compared with intestinal-type. In diffuse-type, MTAP hypermethylation was associated with female gender. CONCLUSION: Our findings show differential gene methylation in tumoral tissue, which allows us to conclude that hypermethylation is associated with gastric carcinogenesis. MTAP promoter hypermethylation can be characterized as a marker of diffuse-type gastric cancer, especially in women and may help in diagnosis, prognosis and therapies. The H pylori infectious agent was present in 44.9% of the samples. This infection may be correlated with the carcinogenic process through the gene promoter hypermethylation, especially the MTAP promoter in diffuse-type. A higher H pylori infection in diffuse-type may be due to greater genetic predisposition. | Mariana Ferreira Leal Eleonidas Moura Lima Patrícia Natália Oliveira Silva Paulo Pimentel Assumpo Danielle Queiroz Calcagno Spencer Luiz Marques Payo Rommel Rodríguez Burbano Marília de Arruda Cardoso Smith | 2007 | World Journal of Gastroenterology2007，13，18： | 24 |
| 3 | Interleukin-1 and TNF-α polymorphisms and Helicobacter pylori in a Brazilian Amazon population显示文摘AIM:To study the association between Interleukin-1(IL-1)and tumor necrosis factor(TNF)-αpolymorphisms,infection by Helicobacter pylori(H pylori)and the development of gastrointestinal diseases.METHODS:Genomic DNA was extracted from the peripheral blood of 177 patients with various gastrointestinal diseases and from 100 healthy volunteers.The polymorphisms in IL-1βand TNF-αgenes were analyzed using the polymerase chain reactionrestriction fragment length polymorphism method(PCRRFLP)and those from IL-1RN with PCR.The presence of infection due to H pylori and the presence of the CagA toxin were detected by serology.The histopathological parameters in the gastric biopsies of the patients were according to the Sydney classification.RESULTS:A comparison of the frequencies of the different polymorphisms studied among the patients and the control group demonstrated that the allele IL1RN*2 was more frequent among patients with gastric ulcers and adenocarcinoma.Carriers of the allele ILRN*2 and those with reactive serology for anti-CagA IgG had a greater risk of developing peptic ulcer and gastric adenocarcinoma,as well as a higher degree of inflammation and neutrophilic activity in the gastric mucosa.CONCLUSION:Our results indicate a positive association between IL-1RN gene polymorphism and infection by positive H pylori CagA strains and the development of gastric ulcers and adenocarcinoma. | Hivana Patricia Melo Barbosa Luisa Caricio Martins Sidney Emanuel Batista dos Santos Samia Demachki Mnica Baraúna Assumpo Charliana Damasceno Arago Tereza Cristina de Oliveira Corvelo | 2009 | World Journal of Gastroenterology2009，15，12： | 17 |
| 4 | MYC and gastric adenocarcinoma carcinogenesis显示文摘MYC is an oncogene involved in cell cycle regulation,cell growth arrest,cell adhesion,metabolism,ribosome biogenesis,protein synthesis,and mitochondrial function. It has been described as a key element of several carcinogenesis processes in humans. Many studies have shown an association between MYC deregulation and gastric cancer. MYC deregulation is also seen in gastric preneoplastic lesions and thus it may have a role in early gastric carcinogenesis. Several studies have suggested that amplification is the main mechanism of MYC deregulation in gastric cancer. In the present review,we focus on the deregulation of the MYC oncogene in gastric adenocarcinoma carcinogenesis,including its association with Helicobacter pylori (H pylori) and clinical applications. | Danielle Queiroz Calcagno Mariana Ferreira Leal Paulo Pimentel Assumpo Marília de Arruda Cardoso Smith Rommel Rodríguez Burbano | 2008 | World Journal of Gastroenterology2008，14，39： | 8 |
| 5 | Clinical implication of 14-3-3 epsilon expression in gastric cancer显示文摘AIM:To evaluate for the first time the protein and mRNA expression of 14-3-3εin gastric carcinogenesis.METHODS:14-3-3εprotein expression was determined by western blotting,and mRNA expression was examined by real-time quantitative RT-PCR in gastric tumors and their matched non-neoplastic gastric tissue samples.RESULTS:Authors observed a significant reduction of 14-3-3εprotein expression in gastric cancer(GC)samples compared to their matched non-neoplastic tissue.Reduced levels of 14-3-3εwere also associated with diffuse-type GC and early-onset of this pathology.Our data suggest that reduced 14-3-3εmay have a role in gastric carcinogenesis process.CONCLUSION:Our results reveal that the reduced 14-3-3εexpression in GC and investigation of 14-3-3ε interaction partners may help to elucidate the carcino-genesis process. | Mariana Ferreira Leal Danielle Queiroz Calcagno Smia Demachki Paulo Pimentel Assumpo Roger Chammas Rommel Rodríguez Burbano Marília de Arruda Cardoso Smith | 2012 | World Journal of Gastroenterology2012，18，13： | 6 |
| 6 | Constrained quadratic state feedback control of discrete-time Markovian jump linear systems显示文摘 | Costa O L V Assump cao Filho E O Boukas E K | 1999 | Automatica1999，35，4： | 1 |
| 7 | Technical option on tetralogy of Fallot with abnormal coronary artery:cases report显示文摘 | Assump(c)(a)o CR Brito Jde D Pfeifer E | | 0，，02： | 1 |
| 8 | Preparation of PdAu/C-Sb205 SnO2 electrocatalysts by borohydride reduction process for direct formic acid fuel cell Ionics 显示文摘 | NANDENHA J DE SOUZA R F B ASSUMP O M H M T SPINACI E V NETO A O | 2013 | Irloics2013，19，9： | 1 |
| 9 | Electro-oxidation of formic acid on Pdlr/C-Sb2 O5 SnOz electrocatalysts prepared by borohydride reduction显示文摘 | NANDENHA J DE SOUZA R F B ASSUMP O M H M T SPINACI E V NETO A O | 2013 | Int J Electrochem Sci2013，8，： | 1 |
| 10 | Interleukin-1β polymorphisms, Helicobacter pyloriinfection in individuals from Northern Brazil with gastric adenocarcinoma显示文摘 | L. Lobo Gatti R. Rodríguez Burbano P. Pimentel de Assump??o M. Arruda Cardoso Smith S. L. Marques Pay?o | 2004 | Clinical and Experimental Medicine2004，，2： | 1 |
| 11 | hTERT methylation and expression in gastric cancer显示文摘 | Carolina Oliveira Gigek Mariana Ferreira Leal Patricia Natalia Oliveira Silva Luara Carolina Frias Lisboa Eleonidas Moura Lima Danielle Queiroz Calcagno Paulo Pimentel Assump??o Rommel Rodriguez Burbano Marilia de Arruda Cardoso Smith | 2009 | Biomarkers2009，，8： | 1 |
| 12 | Impact of previous thyroid autoimmune diseases on prognosis of patients with well-differentiated thyroid cancer显示文摘 | Souza SL Montalli Da Assump??o LV Ward LS | 2003 | Thyroid2003，13，5： | 1 |
| 13 | Addition of Water-soluble Soy Extract and Probiotic Culture, Viscosity, Water Retention Capacity and Syneresis Characteristics of Goat Milk Yogurt 显示文摘 | SILVA D C G ABREU L R ASSUMP G M P | 2012 | Ci-ncia Rural2012，42，3： | 1 |
| 14 | Effect of variation of trans-fatty acid in lactating rats diet on lipoprotein lipase activity in mammary gland,liver,and adipose tissue显示文摘 | Assump o RP dos Santos FD de Mattos Machado AndradeP | 2004 | Nutrition2004，20，： | 1 |
| 15 | Interleukin-10 but not interleukin-18 may be associated with the immune response against well-differentiatedthyroid cancer显示文摘 | Cunha LL Tincani AJ Assump??o LV | 2011 | Clinics(Sao Paulo)2011，66，7： | 1 |
| 16 | Establishment and conventional cytogenetic characterization of three gastric cancer cell lines显示文摘 | Mariana Ferreira Leal José Luiz Martins do Nascimento Carla Elvira Araújo da Silva Maria Fernanda Vita Lamar?o Danielle Queiroz Calcagno André Salim Khayat Paulo Pimentel Assump??o Isabel Rosa Cabral Marília de Arruda Cardoso Smith Rommel Rodríguez Burban | 2009 | Cancer Genetics and Cytogenetics2009，，1： | 1 |
| 17 | Impact of previous thyroid autoimmune diseases on prognosis of patients with well-differentiated thyroid cancer显示文摘 | Lisboa Souza S Vera Montalli da Assump(ca)o L Sterian Ward L | 2003 | Thyroid2003，13，5： | 1 |
| 18 | The use of reverse transcription-polymerase chain reaction (RT-PCR) for the rapid detection and identification of dengue virus in an endemic region: a validation study显示文摘 | Sérgio Oliveira De Paula Roberto J. Pires^Neto Joseane A.C. Tocantins Corrêa Silvia R. Assump??o Márcia L.S. Costa Danielle Malta Lima Benedito Ant?nio Lopes Fonseca | 2002 | Transactions of the Royal Society of Tropical Medicine and Hygiene2002，，3： | 1 |
| 19 | Mechanical behaviour of a prototype orthodontic retraction spring:a numerical-experimental study显示文摘 | Ferreira MA Assump珓ao R Luersen MA | 2013 | Eur J Orthod2013，35，4： | 1 |
| 20 | Gene rearrangement study for minimal residual disease monitoring in children with acute lymphocytic leukemia显示文摘 | Assump?oJG PaulaFD XavierSG | 2013 | Rev Bras Hematol Hemoter2013，35，5： | 1 |
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