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| 1 | Autophagy in bone homeostasis and the onset of osteoporosis显示文摘Autophagy is an evolutionarily conserved intracellular process,in which domestic cellular components are selectively digested for the recycling of nutrients and energy.This process is indispensable for cell homeostasis maintenance and stress responses.Both genetic and functional studies have demonstrated that multiple proteins involved in autophagic activities are critical to the survival,differentiation,and functioning of bone cells,including osteoblasts,osteocytes,and osteoclasts.Dysregulation at the level of autophagic activity consequently disturbs the balance between bone formation and bone resorption and mediates the onset and progression of multiple bone diseases,including osteoporosis.This review aims to introduce the topic of autophagy,summarize the understanding of its relevance in bone physiology,and discuss its role in the onset of osteoporosis and therapeutic potential. | Xing Yin Chenchen Zhou Jingtao Li Renkai Liu Bing Shi Quan Yuan Shujuan Zou | 2019 | Bone Research2019，7，4： | 25 |
| 2 | Generation of haploid embryonic stem cells from Macaca fascicularis monkey parthenotes显示文摘在从经由单性生殖和男性单性生殖的老鼠的胚胎的干细胞(haESCs ) 启用了在修改基因的动物的哺乳动物的房间和代的基因屏蔽的 haploid 的推导的最近的成功。然而, haESCs 是否能从首领被导出,仍然保持未知。这里,我们从 Macaca fascicularis 猴子的单性生殖的胚囊报导 haESCs 的推导。这些房间,作为 PG-haESCs 称为,是 pluripotent 并且能在 vitro 或在 vivo 区分到三胚胎的细菌层的房间。有趣地,一根猴子 PG-haESC 线(MPH1 ) 的 haploidy 与另一个(MPH2 ) 的相比是更稳定的,作为没有排序的激活荧光的房间(FACS ) ,由 haploid 房间的存在出现超过 140 天 haploid 房间的丰富。重要地,转基因的猴子 PG-haESC 线能被 lentivirus- 和 piggyBac 产生调停 transposon 的基因转移。而且,基因屏蔽在猴子 PG-haESCs 是可行的。我们的结果证明 PG-haESCs 能从猴子被产生,提供为在首领的基因分析的一个理想的工具。 | Hui Yang Zhen Liu Yu Ma Cuiqing Zhong Qi Yin Chikai Zhou Linyu Shi Yijun Cai Hanzhi Zhao Hui Wang Fan Tang Yan Wang Chenchen Zhang Xin-yuan Liu Dongmei Lai Ying Jin Qiang Sun Jinsong Li | 2013 | Cell Research2013，23，10： | 22 |
| 3 | Determination of 15 phthalate esters in air by gas-phase and particle-phase simultaneous sampling显示文摘Based on previous research,the sampling and analysis methods for phthalate esters(PAEs)were improved by increasing the sampling flow of indoor air from 1 to 4 L/min,shortening the sampling duration from 8 to 2 hr. Meanwhile,through the optimization of chromatographic conditions,the concentrations of 9 additional PAE pollutants in indoor air were measured. The optimized chromatographic conditions required a similar amount of time for analysis as before,but gave high responsivity,the capability of simultaneously distinguishing 15 kinds of PAEs,and a high level of discrimination between individual sample peaks,as well as stable peak generation. The recovery rate of all gas-phase and particle-phase samples of the 15 kinds of PAEs ranged from 91.26% to 109.42%,meeting the quantitative analysis requirements for indoor and outdoor air sampling and analysis. For the first time,investigation of the concentration levels as well as characteristics of 15 kinds of PAEs in the indoor air from four different traffic micro-environments(private vehicles,busses,taxis and subways)was carried out,along with validation of the optimized sampling and analytical method. The results show that all the 9 additional PAEs could be detected at relatively high pollution levels in the indoor air from the four traffic micro-environments. As none of the pollution levels of the 15 kinds of PAEs in the indoor air from the 4 traffic micro-environments should be neglected,it is of great significance to increase the types of PAEs able to be detected in indoor air. | Chenchen Chi Meng Xia Chen Zhou Xueqing Wang Mili Weng Xueyou Shen | 2017 | Journal of Environmental Sciences2017，29，5： | 7 |
| 4 | Advanced smart biomaterials and constructs for hard tissue engineering and regeneration显示文摘Hard tissue repair and regeneration cost hundreds of billions of dollars annually worldwide, and the need has substantially increased as the population has aged. Hard tissues include bone and tooth structures that contain calcium phosphate minerals.Smart biomaterial-based tissue engineering and regenerative medicine methods have the exciting potential to meet this urgent need. Smart biomaterials and constructs refer to biomaterials and constructs that possess instructive/inductive or triggering/stimulating effects on cells and tissues by engineering the material's responsiveness to internal or external stimuli or have intelligently tailored properties and functions that can promote tissue repair and regeneration. The smart material-based approaches include smart scaffolds and stem cell constructs for bone tissue engineering; smart drug delivery systems to enhance bone regeneration; smart dental resins that respond to pH to protect tooth structures; smart pH-sensitive dental materials to selectively inhibit acid-producing bacteria; smart polymers to modulate biofilm species away from a pathogenic composition and shift towards a healthy composition; and smart materials to suppress biofilms and avoid drug resistance. These smart biomaterials can not only deliver and guide stem cells to improve tissue regeneration and deliver drugs and bioactive agents with spatially and temporarily controlled releases but can also modulate/suppress biofilms and combat infections in wound sites. The new generation of smart biomaterials provides exciting potential and is a promising opportunity to substantially enhance hard tissue engineering and regenerative medicine efficacy. | Ke Zhang Suping Wang Chenchen Zhou Lei Cheng Xianling Gao Xianju Xie Jirun Sun Haohao Wang Michael D.Weir Mark A.Reynolds Ning Zhang Yuxing Bai Hockin H.K.Xu | 2018 | Bone Research2018，6，4： | 6 |
| 5 | Establishment and application of distant hybridization technology in fish显示文摘Hybridization is widely used. However, for a long time, systematic theories and technologies related to hybridization in fish have been lacking. In this study, through long-term systematic research, we investigated and obtained the main rules regarding inheritance and reproduction related to fish distant hybridization. Furthermore, we established one-step and multistep breeding technologies that were suitable for interspecific hybridization and intraspecific hybridization. Simultaneously, we used these two breeding technologies to produce a batch of diploid fish lineages and tetraploid fish lineages and improved fishes. In addition, we widely discuss the methods, technologies and results of hybridization breeding, referring to the domestic and foreign literature on fish hybridization. We hope that this paper will be beneficial for the research and application of fish hybrid breeding. | Shi Wang Chenchen Tang Min Tao Qinbo Qin Chun Zhang Kaikun Luo Rurong Zhao Jing Wang Li Ren Jun Xiao Fangzhou Hu Rong Zhou Wei Duan Shaojun Liu | 2019 | Science China(Life Sciences)2019，62，1： | 6 |
| 6 | DNA N^6-methyladenine demethylase ALKBH1 enhances osteogenic differentiation of human MSCs显示文摘ALKBH1 was recently discovered as a demethylase for DNA N^6-methyladenine(N6-m A), a new epigenetic modification, and interacts with the core transcriptional pluripotency network of embryonic stem cells.However, the role of ALKBH1 and DNA N6-m A in regulating osteogenic differentiation is largely unknown.In this study, we demonstrated that the expression of ALKBH1 in human mesenchymal stem cells(MSCs)was upregulated during osteogenic induction. Knockdown of ALKBH1 increased the genomic DNA N6-m A levels and significantly reduced the expression of osteogenic-related genes, alkaline phosphatase activity, and mineralization. ALKBH1-depleted MSCs also exhibited a restricted capacity for bone formation in vivo.By contrast, the ectopic overexpression of ALKBH1 enhanced osteoblastic differentiation. Mechanically,we found that the depletion of ALKBH1 resulted in the accumulation of N6-m A on the promoter region of ATF4, which subsequently silenced ATF4 transcription. In addition, restoring the expression of ATP by adenovirus-mediated transduction successfully rescued osteogenic differentiation. Taken together, our results demonstrate that ALKBH1 is indispensable for the osteogenic differentiation of MSCs and indicate that DNA N6-m A modifications area new mechanism for the epigenetic regulation of stem cell differentiation. | Chenchen Zhou Yuting Liu Xiaobing Li Jing Zou Shujuan Zou | 2016 | Bone Research2016，4，3： | 6 |
| 7 | Heavy mineral assemblage characteristics and the Cenozoic paleogeographic evolution in southwestern Qaidam Basin显示文摘Based on the analysis of heavy mineral assemblages in Cenozoic southwestern Qaidam Basin, we found that different areas have variable heavy mineral assemblage characteristics, which suggested that there were two source areas—the Altyn Mountains and the Qimen Tagh-East Kunlun Mountains. In Ganchaigou-Shizigou-Huatugou(Area A), which was mainly source from the Altyn Mountains, its heavy minerals were mainly composed of zircon, Ti-oxides, and wollastonite in the Paleoceneearly Eocene and mainly of unstable minerals, especially amphibole, in the middle Eocene-Oligene. Since the late OligoceneMiocene, the heavy minerals were still mainly unstable minerals, but the content of epidote increased and the content of amphibole decreased. In Qigequan-Hongliuquan(Area B), which was the mixed source from the Altyn Mountains and the Qimen Tagh-East Kunlun Mountains, its heavy minerals were mainly garnet, epidote, and amphibole. The source of LücaotanDongchaishan-Kunbei(Area C) was mainly from the Qimen Tagh-East Kunlun Mountains, heavy minerals in the sediments in Area C were mainly zircon and Ti-oxides in Paleogene and garnet, epidote, and amphibole in Neogene. In Yuejin-Youshashan(Area D), where the stable minerals and unstable minerals were present simultaneously, the heavy mineral assemblages was controlled by multi-direction source. The variation of heavy mineral assemblages in southwestern Qaidam Basin shows that Altyn Mountains was of low-lying topographic relief in Paleocene-early Eocene, and the rapid uplift of Altyn Mountains started from the middle Eocene. In Paleogene, the Altyn Tagh Fault had a slow strike-slip velocity, but the strike-slip velocity increased greatly since the late Oligocene, leading to a strike-slip displacement above 300 km since Neogene. Meanwhile, the Qimen Tagh-East Kunlun fault zone was under a stable tectonic stage in Paleogene with the Qimen Tagh Mountain being lowlying hills; since the late Oligocene, the fault zone started to activate and the Qimen Tagh Mountain began to uplift rapidly. | LI LinLin GUO ZhaoJie GUAN ShuWei ZHOU SuPing WANG MingZhen FANG YaNan ZHANG ChenChen | 2015 | Science China Earth Sciences2015，58，6： | 6 |
| 8 | Quantitative analysis of Pb adsorption on sulfhydryl-modified biochar显示文摘Biochar is an effective absorbent for remediating heavy metal contaminated soil,but functional optimization is still needed to improve its performance in field application.Here,we characterized the physical structures and surface chemical properties of raw wood biochar and palm biochar(WB and PB)and the corresponding sulfhydryl-modified biochar(SWB and SPB).Their adsorption capacity for Pb was evaluated by combining thermodynamic and kinetic adsorption at 0.01 mol/L KCl and corresponding model simulation.The results demonstrated successful grafting of sulfhydryl groups onto the biochar,which dramatically reduced the specific surface area(SSA)and pore volume of biochar.The pKa in the surface complexation model(SCM)indicated similar proton affinity between sulfhydryl groups and original functional groups on the biochar.SCM could satisfactorily fit the Pb adsorption behaviors,and model analysis revealed that Pb tended to be adsorbed on low-proton affin-ity sites at low pH,but high-proton affinity sites became dominant in Pb adsorption with increasing pH and adsorbed almost all Pb ions at pH>7.0.Besides,the Pb adsorption density of SWB and SPB was improved by 8.86 and 3.64 folds relative to that of WB and PB,respectively.Over 90% of initially added Pb ions were removed in 1440 and 720 min by raw and sulfhydryl-modified biochar,respectively,indicating that sulfhydryl modification accelerated the Pb adsorption of biochar.These results suggest that site density,SSA and pore structure of biochar play crucial roles in heavy metal adsorption,and sulfhydryl modification may improve the performance of biochar in remediating heavy metal contaminated soil. | Juan Xiong Mengge Zhou Chenchen Qu Daohai Yu Chang Chen Mingxia Wang Wenfeng Tan | 2021 | Biochar2021，3，1： | 5 |
| 9 | Analysis of the influencing factors of PAEs volatilization from typical plastic products显示文摘The primary emphasis of this research was to investigate the foundations of phthalate(PAEs) pollutant source researches and then firstly confirmed the concept of the coefficient of volatile strength, namely phthalate total content in per unit mass and unit surface area of pollutant sources. Through surveying and evaluating the coefficient of volatile strength of PAEs from typical plastic products, this research carried out reasonable classification of PAEs pollutant sources into three categories and then investigated the relationship amongst the coefficient of volatile strength as well as other environmental factors and the concentration level of total PAEs in indoor air measured in environment chambers.Research obtained phthalate concentration results under different temperature, humidity,the coefficient of volatile strength and the closed time through the chamber experiment. In addition, this study further explored the correlation and ratio of influencing factors that affect the concentration level of total PAEs in environment chambers, including environmental factors, the coefficient of volatile strengths of PAEs and contents of total PAEs in plastic products. The research created an improved database system of phthalate the coefficient of volatile strengths of each type of plastic goods, and tentatively revealed that the volatile patterns of PAEs from different typical plastic goods, finally confirmed that the coefficient of volatile strengths of PAEs is a major factor that affects the indoor air total PAEs concentration, which laid a solid foundation for further establishing the volatile equation of PAEs from plastic products. | Weidong Chen Chenchen Chi Chen Zhou Meng Xia Cees Ronda Xueyou Shen | 2018 | Journal of Environmental Sciences2018，30，4： | 5 |
| 10 | One Step Quick Detection of Cancer Cell Surface Marker by Integrated NiFe-based Magnetic Biosensing Cell Cultural Chip显示文摘RGD peptides has been used to detect cell surface integrin and direct clinical effective therapeutic drug selection. Herein we report that a quick one step detection of cell surface marker that was realized by a specially designed NiF e-based magnetic biosensing cell chip combined with functionalized magnetic nanoparticles. Magnetic nanoparticles with 20-30 nm in diameter were prepared by coprecipitation and modified with RGD-4C, and the resultant RGD-functionalized magnetic nanoparticles were used for targeting cancer cells cultured on the NiF e-based magnetic biosensing chip and distinguish the amount of cell surface receptor-integrin.Cell lines such as Calu3, Hela, A549, CaF br, HEK293 and HUVEC exhibiting different integrin expression were chosen as test samples. Calu3, Hela, HEK293 and HUVEC cells were successfully identified. This approach has advantages in the qualitative screening test. Compared with traditional method, it is fast, sensitive, low cost,easy-operative, and needs very little human intervention. The novel method has great potential in applications such as fast clinical cell surface marker detection, and diagnosis of early cancer, and can be easily extended to other biomedical applications based on molecular recognition. | Chenchen Bao Lei Chen Tao Wang Chong Lei Furong Tian Daxiang Cui Yong Zhou | 2013 | Nano-Micro Letters2013，5，3： | 5 |
| 11 | AFF4 regulates osteogenic differentiation of human dental follicle cells显示文摘As a member of the AFF(AF4/FMR2)family,AFF4 is a transcription elongation factor that is a component of the super elongation complex.AFF4 serves as a scaffolding protein that connects transcription factors and promotes gene transcription through elongation and chromatin remodelling.Here,we investigated the effect of AFF4 on human dental follicle cells(DFCs)in osteogenic differentiation.In this study,we found that small interfering RNA-mediated depletion of AFF4 resulted in decreased alkaline phosphatase(ALP)activity and impaired mineralization.In addition,the expression of osteogenic-related genes(DLX5,SP7,RUNX2 and BGLAP)was significantly downregulated.In contrast,lentivirus-mediated overexpression of AFF4 significantly enhanced the osteogenic potential of human DFCs.Mechanistically,we found that both the mRNA and protein levels of ALKBH1,a critical regulator of epigenetics,changed in accordance with AFF4 expression levels.Overexpression of ALKBH1 in AFF4-depleted DFCs partially rescued the impairment of osteogenic differentiation.Our data indicated that AFF4 promoted the osteogenic differentiation of DFCs by upregulating the transcription of ALKBH1. | Qingyue Xiao Yuning Zhang Xingying Qi Yaqian Chen Rui Sheng Ruoshi Xu Quan Yuan Chenchen Zhou | 2020 | International Journal of Oral Science2020，12，4： | 4 |
| 12 | Runxl protects against the pathological progression of osteoarthritis显示文摘Runt-related transcription factor-1(Runxl)is required for chondrocyte-to-osteoblast lineage commitment by enhancing both chondrogenesis and osteogenesis during vertebrate development.However,the potential role of Runxl in joint diseases is not well known.In the current study,we aimed to explore the role of Runxl in osteoarthritis induced by anterior cruciate ligament transaction(ACLT)surgery.We showed that chondrocyte-specific Runxl knockout(Runx1f/fCol2a1-Cre)aggravated cartilage destruction by accelerating the loss of proteoglycan and collagen II in early osteoarthritis.Moreover,we observed thinning and ossification of the growth plate,a decrease in chondrocyte proliferative capacity and the loss of bone matrix around the growth plate in late osteoarthritis.We overexpressed Runxl by adeno-associated virus(AAV)in articular cartilage and identified its protective effect by slowing the destruction of osteoarthritis in cartilage in early osteoarthritis and alleviating the pathological progression of growth plate cartilage in late osteoarthritis.ChIP-seq analysis identified new targets that interacted with Runxl in cartilage pathology,and we confirmed the direct interactions of these factors with Runxl by ChIP-qPCR.This study helps us to understand the function of Runxl in osteoarthritis and provides new clues for targeted osteoarthritis therapy. | Chenchen Zhou Yujia Cui Yueyi Yang Daimo Guo Demao Zhang Yi Fan Xiaobing Li Jing Zou Jing Xie | 2021 | Bone Research2021，9，4： | 4 |
| 13 | Exosomal PD-L1 induces osteogenic differentiation and promotes fracture healing by acting as an immunosuppressant显示文摘A moderate inflammatory response at the early stages of fracture healing is necessary for callus formation.Over-active and continuous inflammation,however,impairs fracture healing and leads to excessive tissue damage.Adequate fracture healing could be promoted through suppression of local over-active immune cells in the fracture site.In the present study,we achieved an enriched concentration of PD-L1 from exosomes(Exos)of a genetically engineered Human Umbilical Vein Endothelial Cell(HUVECs),and demonstrated that exosomes overexpressing PD-L1 specifically bind to PD-1 on the T cell surface,suppressing the activation of T cells.Furthermore,exosomal PD-L1 induced Mesenchymal Stem Cells(MSCs)towards osteogenic differentiation when pre-cultured with T cells.Moreover,embedding of Exos into an injectable hydrogel allowed Exos delivery to the surrounding microenvironment in a time-released manner.Additionally,exosomal PD-L1,embedded in a hydrogel,markedly promoted callus formation and fracture healing in a murine model at the early over-active inflammation phase.Importantly,our results suggested that activation of T cells in the peripheral lymphatic tissues was inhibited after local administration of PD-L1-enriched Exos to the fracture sites,while T cells in distant immune organs such as the spleen were not affected.In summary,this study provides the first example of using PD-L1-enriched Exos for bone fracture repair,and highlights the potential of Hydrogel@Exos systems for bone fracture therapy through immune inhibitory effects. | Ze Lin Yuan Xiong Weilin Meng Yiqiang Hu Lili Chen Lang Chen Hang Xue Adriana CPanayi Wu Zhou Yun Sun Faqi Cao Guodong Liu Liangcong Hu Chenchen Yan Xudong Xie Chuanchuan Lin Kaiyong Cai Qian Feng Bobin Mi Guohui Liu | 2022 | Bioactive Materials2022，7，7： | 4 |
| 14 | Parathyroid hormone increases alveolar bone homoeostasis during orthodontic tooth movement in rats with periodontitis via crosstalk between STAT3 and β-catenin显示文摘Periodontitis patients are at risk of alveolar bone loss during orthodontic treatment.The aim of this study was to investigate whether intermittent parathyroid hormone(1–34)treatment(iPTH)could reduce alveolar bone loss during orthodontic tooth movement(OTM)in individuals with periodontitis and the underlying mechanism.A rat model of OTM in the context of periodontitis was established and alveolar bone loss was observed.The control,iPTH and iPTH+stattic groups received injections of vehicle,PTH and vehicle,or PTH and the signal transducer and activator of transcription 3(STAT3)inhibitor stattic,respectively.iPTH prevented alveolar bone loss by enhancing osteogenesis and suppressing bone resorption in the alveolar bone during OTM in rats with periodontitis.This effect of iPTH was along with STAT3 activation and reduced by a local injection of stattic.iPTH promoted osteoblastic differentiation and might further regulate the Wnt/β-catenin pathway in a STAT3-dependent manner.The findings of this study suggest that iPTH might reduce alveolar bone loss during OTM in rats with periodontitis through STAT3/β-catenin crosstalk. | Cheng Zhang Tiancheng Li Chenchen Zhou Li Huang Yuyu Li Han Wang Peipei Duan Shujuan Zou Li Mei | 2020 | International Journal of Oral Science2020，12，4： | 4 |
| 15 | Klotho in Osx+-mesenchymal progenitors exerts pro-osteogenic and anti-inflammatory effects during mandibular alveolar bone formation and repair显示文摘Maxillofacial bone defects are commonly seen in clinical practice.A clearer understanding of the regulatory network directing maxillofacial bone formation will promote the development of novel therapeutic approaches for bone regeneration.The fibroblast growth factor(FGF)signalling pathway is critical for the development of maxillofacial bone.Klotho,a type I transmembrane protein,is an important components of FGF receptor complexes.Recent studies have reported the presence of Klotho expression in bone.However,the role of Klotho in cranioskeletal development and repair remains unknown.Here,we use a genetic strategy to report that deletion of Klotho in Osx-positive mesenchymal progenitors leads to a significant reduction in osteogenesis under physiological and pathological conditions.Klotho-deficient mensenchymal progenitors also suppress osteoclastogenesis in vitro and in vivo.Under conditions of inflammation and trauma-induced bone loss,we find that Klotho exerts an inhibitory function on inflammation-induced TNFR signaling by attenuating Rankl expression.More importantly,we show for the first time that Klotho is present in human alveolar bone,with a distinct expression pattern under both normal and pathological conditions.In summary,our results identify the mechanism whereby Klotho expressed in Osx+-mensenchymal progenitors controls osteoblast differentiation and osteoclastogenesis during mandibular alveolar bone formation and repair.Klotho-mediated signaling is an important component of alveolar bone remodeling and regeneration.It may also be a target for future therapeutics. | Yi Fan Chen Cui Clifford JRosen Tadatoshi Sato Ruoshi Xu Peiran Li Xi Wei Ruiye Bi Quan Yuan Chenchen Zhou | 2022 | Signal Transduction and Targeted Therapy2022，7，6： | 3 |
| 16 | Compliant substratum modulates vinculin expression in focal adhesion plaques in skeletal cells显示文摘The biophysical properties of the extracellular matrix (ECM) dictate tissue-specific cell behaviour.In the skeleton system,bone shows the potential to adapt its architecture and contexture to environmental rigidity via the bone remodelling process,which involves chondrocytes,osteoblasts,osteoclasts,osteocytes and even peripheral bone marrow-derived stem/stromal cells (BMSCs).In the current study,we generated stiff (~1 014 ± 56) kPa,Young’s modulus) and soft (~46 ± 11) kPa silicon-based elastomer polydimethylsiloxane (PDMS) substrates by mixing curing agent into oligomeric base at 1:5 and 1:45 ratios,respectively,and investigated the influence of substrate stiffness on the cell behaviours by characterizing cell spreading area,cell cytoskeleton and cell adhesion capacity.The results showed that the cell spreading areas of chondrocytes,osteoblasts,osteoclasts,osteocytes and BMSCs were all reduced in the soft substrate relative to those in the stiff substrate.F-actin staining confirmed that the cytoskeleton was also changed in the soft group compared to that in the stiff group.Vinculin in focal adhesion plaques was significantly decreased in response to soft substrate compared to stiff substrate.This study establishes the potential correlation between microenvironmental mechanics and the skeletal system,and the results regarding changes in cell spreading area,cytoskeleton and cell adhesion further indicate the important role of biomechanics in the cell-matrix interaction. | Chenchen Zhou Qingxuan Wang Demao Zhang Linyi Cai Wei Du Jing Xie | 2019 | International Journal of Oral Science2019，11，2： | 3 |
| 17 | Single-cell transcriptomic landscape of human blood cells显示文摘High throughput single-cell RNA-seq has been successfully implemented to dissect the cellular and molecular features underlying hematopoiesis.However,an elaborate and comprehensive transcriptome reference of the whole blood system is lacking.Here,we profiled the transcriptomes of 7551 human blood cells representing 32 immunophenotypic cell types,including hematopoietic stem cells,progenitors and mature blood cells derived from 21 healthy donors.With high sequencing depth and coverage,we constructed a single-cell transcriptional atlas of blood cells(ABC) on the basis of both protein-coding genes and long noncoding RNAs(lncRNAs),and showed a high consistence between them.Notably,putative lncRNAs and transcription factors regulating hematopoietic cell differentiation were identified.While common transcription factor regulatory networks were activated in neutrophils and monocytes,lymphoid cells dramatically changed their regulatory networks during differentiation.Furthermore,we showed a subset of nucleated erythrocytes actively expressing immune signals,suggesting the existence of erythroid precursors with immune functions.Finally,a web portal offering transcriptome browsing and blood cell type prediction has been established.Thus,our work provides a transcriptional map of human blood cells at single-cell resolution,thereby offering a comprehensive reference for the exploration of physiological and pathological hematopoiesis. | Xiaowei Xie Mengyao Liu Yawen Zhang Bingrui Wang Caiying Zhu Chenchen Wang Qing Li Yingying Huo Jiaojiao Guo Changlu Xu Linping Hu Aiming Pang Shihui Ma Una Wang Wenbin Cao Shulian Chen Qiuling Li Sudong Zhang Xueying Zhao Wen Zhou Hongbo Luo Guoguang Zheng Erlie Jiang Sizhou Feng Lixiang Chen Lihong Shi Hui Cheng Sha Hao Ping Zhu Tao Cheng | 2021 | National Science Review2021，8，3： | 3 |
| 18 | Dielectr ic barr ier discharge plasma-assisted modification of g-C_(3)N_(4)/Ag_(2)O/TiO_(2)-NRs composite enhanced photoelectrocatalytic activity显示文摘Dielectric barrier discharge(DBD)plasma applied as surface treatment technology was employed for the modification of Ag_(2)O and graphitic carbon nitride(g-C_(3)N_(4))powders.Subsequently,the pretreated powders were sequentially loaded onto TiO_(2)nanorods(TiO_(2)-NRs)via electro-deposition,followed by calcination at N_(2)atmosphere.The results indicated that at the optimal plasma discharge time of 5 min for modification of g-C_(3)N_(4)and Ag_(2)O,photocurrent density of ternary composite was 6 times to bare TiO_(2)-NRs under UV-visible light irradiation.Phenol was degraded by using DBD plasma-modified g-C_(3)N_(4)/Ag_(2)O/TiO_(2)-NRs electrode to analyze the photoelectrocatalytic performance.The removal rate of phenol for g-C_(3)N_(4)-5/Ag_(2)O-5/TiO_(2)-NRs electrode was about 3.07 times to that for TiO_(2)-NRs electrode.During active species scavengers'analysis,superoxide radicals and hydroxyl radicals were the main oxidation active species for pollutants degradation.A possible electron-hole separation and transfer mechanism of ternary composite with high photoelectrocatalytic performance was proposed. | Yupei Long Chenchen Yuan Xiaomin Wang Dongyan Jin Hong Zhou Qiongyin Wang Chenyang Lu Yuqi Chen Yanqing Cong Qi Wang Yi Zhang | 2021 | Journal of Environmental Sciences2021，33，6： | 2 |
| 19 | GRP78 inhibits macrophage adhesion via SR-A显示文摘Class A scavenger receptor(SR-A)plays an important role in macrophage adhesion.However,the underlying mechanism remains unclear.We previously found that 78 kDa glucose-regulated protein(GRP78)inhibited SRA-mediated ligand internalization into macrophage by binding to SR-A.The aim of the study was to investigate whether GRP78 could regulate SR-A-mediated cell adhesion.We demonstrated that GRP78 bound directly to SR-A by fluorescence resonance energy transfer(FRET)assay.Overexpression of GRP78 inhibited macrophage adhesion via SR-A.These results suggest that GRP78 may act as an inhibitor of macrophage adhesion via SR-A. | Hui Bai Nan Li Xiaodan Zhou Chenchen Wang Yan Zhang Xudong Zhu Min Huang Yaoyu Chen Xiaoyu Li Qing Yang Chaojun Li Jingjing Ben Qi Chen | 2014 | The Journal of Biomedical Research2014，28，4： | 2 |
| 20 | 18F-FLT PET/CT imaging is not competent for the pretreatment evaluation of metastatic gastric cancer: a comparison with 18F-FDG PET/CT imaging显示文摘 | Min Zhou Chenchen Wang Silong Hu Yongping Zhang Zhifeng Yao Jin Li Weijian Guo Yingjian Zhang | 2013 | Nuclear Medicine Communications2013，，7： | 2 |
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