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| 1 | From Golden Rice to aSTARice:Bioengineering Astaxanthin Biosynthesis in Rice Endosperm显示文摘Carotenoids are important phytonutrients with antioxidant properties,and are widely used in foods and feedstuffs as Supplements.Astaxanthin,a red-colored ketocarotenoid,has strong antioxidant activity and thus can benefit human health.However,astaxanthin is not produced in most higher plants.Here we report the bioengineering of astaxanthin biosynthesis in rice endosperm by introducing four synthetic genes,sZmPSY1,sPaCrtl,sCrBKT,and sHpBHY,which encode the enzymes phytoene synthase,phytoene desaturase,β-carotene ketolase,and β-carotene hydroxylase,respectively.Transgneic overexpression of two (sZmPSY1 and sPaCrtl),three (sZmPSY1,sPaCrtl and sCrBKT),and all these four genes driven by rice endosperm-specific promoters established the Carotenoid/ketocarotenoid/astaxanthin biosynthetic pathways in the endosperm and thus resulted in various types of germplasm,from the yellow-grained β-caro- tene-enriched Golden Rice to orange-red-grained Canthaxanthin Rice and Astaxanthin Rice,respectively. Grains Of Astaxanthin Rice were enriched with astaxanthin in the endosperm and had higher antioxidant activity.These results proved that introduction of a minimal set of four transgenes enables de novo biosynthesis of astaxanthin in therice endosperm.This work provides a Successful example for synthetic biology in plants and biofortification in crops;the biofortified rice products generated by this study could be consumed as health-promoting foods and processed tO produce dietary supplements. | Qinlong Zhu Dongchang Zeng Suize Yu Chaojun Cui Jiamin Li Heying Li Junyu Chen Runzhao Zhang Xiucai Zhao Letian Chen Yao-Guang Liu | 2018 | Molecular Plant2018，11，12： | 35 |
| 2 | Coal and gas outburst dynamic system显示文摘Coal and gas outburst is an extremely complex dynamic disaster in coal mine production process which will damage casualties and equipment facilities, and disorder the ventilation system by suddenly ejecting a great amount of coal and gas into roadway or working face. This paper analyzed the interaction among the three essential elements of coal and gas outburst dynamic system. A stress-seepage-damage coupling model was established which can be used to simulate the evolution of the dynamical system, and then the size scale of coal and gas outburst dynamical system was investigated. Results show that the dynamical system is consisted of three essential elements, coal-gas medium(material basis), geology dynamic environment(internal motivation) and mining disturbance(external motivation). On the case of C13 coal seam in Panyi Mine, the dynamical system exists in the range of 8–12 m in front of advancing face. The size scale will be larger where there are large geologic structures. This research plays an important guiding role for developing measures of coal and gas outburst prediction and prevention. | Fan Chaojun Li Sheng Luo Mingkun Du Wenzhang Yang Zhenhua | 2017 | International Journal of Mining Science and Technology2017，27，1： | 19 |
| 3 | A small natural molecule promotes mitochondrial fusion through inhibition of the deubiquitinase USP30显示文摘Mitochondrial 熔化是为正常 mitochondrial 功能和 mitochondrial DNA 继承混合并且统一 mitochondrial 分隔空间的一个高度协调的过程。Dysregulated mitochondrial 熔化原因 mitochondrial 破碎,反常 mitochondrial 生理学和继承,并且有原因地与很多 neuronal 疾病被连接了。这里,我们识别了 potently 导致了 mitochondrial 熔化在在 Mfn1 或 Mfn2 是缺乏的房间恢复 mitochondrial 网络和氧化呼吸的 diterpenoid 衍生物 15-oxospiramilactone (S3 ) 。线粒体局部性的 deubiquitinase USP30 是 S3 的一个目标。由 S3 的 USP30 的抑制导致 Mfn1/2 的 non-degradative ubiquitination 的增加,它提高 Mfn1 和 Mfn2 活动并且支持 mitochondrial 熔化。通过 USP30 的一个禁止者的使用,因此,我们的学习揭开在支持 mitochondrial 的 Mfns 的 non-degradative ubiquitination 的异乎寻常的功能熔化。 | Wen Yue Ziheng Chen HaiYang Li Chen Yan Ming Chen Du Feng Chaojun Yan Hao Wu Lei Du Yueying Wang Jinhua Liu Xiaohu Huang Laixin Xia Lei Liu Xiaohui Wang Haijing Jin Jun Wang Zhiyin Song Xiaojiang Hao Quan Chen | 2014 | Cell Research2014，24，4： | 18 |
| 4 | Overexpression of cotton (Gossypium hirsutum) dirigentl gene enhances lignification that blocks the spread of Verticillium dahliae显示文摘Dirigent 总科在整个植物王国丰富,特别脉管的植物。阐明棉花(Gossypium hirsutum ) 的功能在木质化的 DIR 基因,编码通常认为的 dirigent 蛋白质的二 cDNAs (指明的 GhDIR1 和 GhDIR2 ) 从棉花 cDNA 图书馆被孤立。实时量的反向的抄写聚合酶链反应分析表明那个 GhDIR1 抄本优先地在棉花胚轴被积累,而 GhDIR2 主要在棉花纤维被表示。GhDIR1 基因的 Overexpression 导致了转基因的棉花植物的木质素内容的增加,与野类型的相比。组织化学的试金表明转基因的植物在外皮和脉管的捆比野类型的显示了更普遍的木质化。而且,转基因的棉花植物显示了更多的忍耐到 Verticillium dahliae 的感染。我们的数据建议 GhDIR1 可以涉及能堵住真菌的病原体 V 的传播的棉花木质化。dahliae。 | Haiyan Shi Zhihao Liu Li Zhu Chaojun Zhang Yun Chen Ying Zhou Fuguang Li Xuebao Li | 2012 | Acta Biochimica et Biophysica Sinica2012，44，7： | 12 |
| 5 | Comparative Proteome Analysis of Human Lung Squamous Carcinoma Tissue显示文摘客观:与高分辨率建立二维的电气泳动侧面;从人的肺的重制度有鳞的癌织物;配对的正常肿瘤邻近的支气管的上皮,;识别微分表示由使用 proteome 分析的联系肿瘤的蛋白质。方法:有 20 人的肺的比较 proteome 分析有鳞的癌纸巾;邻近肿瘤的配对的正常支气管的上皮被执行。人的肺的全部的蛋白质有鳞的癌织物;配对的正常肿瘤邻近的支气管的上皮借助于使不能调动的 pH 被分开基于坡度的二维的胶化电气泳动(2-DE ) ;染色的银。微分表示蛋白质被分析;然后到飞行团分光术(MALDI-TOF-MS ) 的帮助矩阵的激光解吸附作用 / 电离时间识别了。结果:(1 ) 人的肺的解决得好的、可再现的 2-DE 模式有鳞的癌;邻近的正常支气管的上皮被获得。为肿瘤织物, 3 胶化的平均的点是 1567 ± 4 6,;4 看到的 1436 ± 5 与 91.6% 的平均匹配率被匹配。为控制, 3 胶化的平均的点是 1349 ± 5 8,;5 看到的 1228 ± 3 与 91.03% 的平均匹配率被匹配。匹配的点的平均位置偏差是在 IEF 的 0.924 ± 0 .128 公里方向,;在 SDS 页方向的 1.022 ± 0 .205 公里;(2 ) 6 看到的 1178 ± 5 的一个总数在 20 人的肺的电泳地图之间被匹配有鳞的癌纸巾;配对正常肿瘤邻近的支气管的上皮。76 差别表示了蛋白质被屏蔽;(3 ) 68 微分蛋白质被 PMF 识别,一些蛋白质是 oncogenes 的产品,;涉及房间的规定的其它骑车;信号转导变异;(4 ) 以便验证识别结果的可靠性, 3 蛋白质 mdm2 的表示, c-jun;EGFR,它与肺被相关有鳞的癌,被免疫检测组织化学的染色;西方的污点分析。结果揭示了那 mdm2, c-jun;EGFR 在肺是起来调整的有鳞的癌而他们在邻近的正常支气管的上皮是下面调整的,正常的肺纸巾;煽动性的假肿瘤,它与我们的 proteome 分析一致结果。结论:人的肺的解决得好的、可再现的 2-DE 模式有鳞的癌;邻近的正常支气管的上皮被建立;68 微分蛋白质被成功地使用比较 proteome 分析描绘。这些结果将为屏蔽使用到 diagnose 的分子的简历标记提供科学基础;对待肺有鳞的癌,以及改进病人的预后;为肺的研究提供新线索有鳞的致癌机制。 | LI Cui TANG Can'e DUAN Chaojun YI Hong XIAO Zhiqiang CHEN Zhuchu | 2006 | The Chinese-German Journal of Clinical Oncology2006，5，4： | 11 |
| 6 | HY5-HDA9 Module Transcriptionally Regulates Plant Autophagy in Response to Light-to-Dark Conversion and Nitrogen Starvation显示文摘Light is arguably one of the most important environmental factors that determines virtually all aspects of plant growth and development,but the molecular link between light signaling and the autophagy pathway has not been elucidated in plants.In this study,we demonstrate that autophagy is activated during light-to-dark conversion though transcriptional upregulation of autophagy-related genes(ATGs).We showed that depletion of the ELONGATED HYPOCOTYL 5(HY5),a key component of light signaling,leads to enhanced autophagy activity and resistance to extended darkness and nitrogen starvation treatments,contributing to higher expression oiATGs.HY5 interacts with and recruits HISTONE DEACETYLASE 9(HDA9)to ATG5 and ATG8e loci to repress their expression by deacetylation of the Lys9 and Lys27 of histone 3.Furthermore,we found that both darkness and nitrogen depletion induce the degradation of HY5 via 26S proteasome and the concomitant disassociation of HDA9 from ATG5 and ATG8e loci,leading to their depression and thereby activated autophagy.Genetic analysis further confirmed that HY5 and HDA9 act synergistically and function upstream of the autophagy pathway.Collectively,our study unveils a previously unknown transcriptional and epigenetic network that regulates autophagy in response to light-to-dark conversion and nitrogen starvation in plants. | Chao Yang Wenjin Shen Lianming Yang Yun Sun Xibao Li Minyi Lai Juan Wei Chaojun Wang Yingchao Xu Faqiang Li Shan Liang Chengwei Yang Shangwei Zhong Ming Luo Caiji Gao | 2020 | Molecular Plant2020，13，3： | 8 |
| 7 | Synthesis of graphene materials by electrochemical exfoliation: Recent progress and future potential显示文摘Synthesis of structurally controlled graphene materials is critical for realizing their practical applications.The electrochemical exfoliation of graphite has emerged as a simple method to produce graphene materials.This review examines research progress in the last 5 years,from 2015 to 2019.Graphene material synthesis methods generally have a trade‐off between increasing production yield and achieving better material property control.The synthesis conditions for synthesizing pristine graphene,graphene oxide(GO),and graphene composites are significantly different.Thus,in this review,we first discuss synthesis methods for graphene materials with high C/O ratios from four aspects:graphite electrodes,equipment engineering,electrolytes,and additional reduction methods.Next,we survey synthesis methods for GO and examine how the pretreatment of the graphite electrodes,electrolytes,and operation parameters,such as applied voltages,electrolyte temperatures,and mechanical forces,affect the quality of GO.Further,we summarize electrochemical exfoliation methods used to dope graphene materials,introduce covalent functional groups,incorporate various nanoparticles,and assembly of graphene architectures.For all synthesis methods,we compare the properties of resulting graphene materials such as C/O ratios,lateral size,layer numbers,and quality characterized by Raman spectroscopy.Lastly,we propose our perspectives on further research.We hope this review stimulates more studies to realize the on‐demand production of graphene materials with desired properties using electrochemical exfoliation methods. | Fei Liu Chaojun Wang Xiao Sui Muhammad Adil Riaz Meiying Xu Li Wei Yuan Chen | 2019 | Carbon Energy2019，1，2： | 6 |
| 8 | Overexpressed BRH1, a RING finger gene, alters rosette leaf shape in Arabidopsis thaliana显示文摘Leaves are the most important plant parts for photosynthesis and respiration. Many genes are involved in determining leaf shape;however, little is known about the effects of brassinosteroid (BR) signaling-pathway genes on the development of leaf shape. Here, the brassinosteroid-responsive RING-H2 (BRH1) gene, which is suppressed by 24-epi-brassinolide treatment, was isolated from Arabidopsis thaliana. The amino acid sequence contained a highly conserved RING finger domain. In a phylogenetic analysis,BRH1 clustered closely with GLYMA11G02470.1. The leaves of brh1 mutant plants were not much different to those of the wild-type, while transgenic plants with high BRH1 expression levels had rounder rosette leaves. Mutants of the BR synthesis pathway also had a similar round leaf phenotype, and greater BRH1 expression levels. Moreover, the related marker genes KNAT1,AtHB13 and ROT4, which are known to control leaf shape, altered transcriptional levels in both transgenic BRH1 and BR-synthesis mutant lines. Thus, BRH1 may be involved in the BR signaling pathway and regulate the growth and development of rosette leaves. Research on BRH1 may prove valuable for understanding the regulatory mechanism of leaf shape and improving the leaf shapes of ornamental plants. | Xiaoqian Wang Eryong Chen Xiaoyang Ge Qian Gong HamamaIslam Butt Chaojun Zhang Zuoren Yang Fuguang Li Xueyan Zhang | 2018 | Science China(Life Sciences)2018，61，1： | 5 |
| 9 | Peripheral Lymphocyte Subsets as a Marker of Parkinson's Disease in a Chinese Population显示文摘In this study, we conducted a clinical analysis of lymphocyte subtypes in 268 patients with Parkinson's disease(PD) to assess their clinical impact as a potential marker of advanced PD in Chinese patients. The participants comprised 268 sporadic PD patients and 268 healthy controls. The numbers of natural killer(NK) cells and CD3+, CD3+CD4+, CD3+CD8+, and CD19+ lymphocytes from peripheral blood were determined by immunostaining and flow cytometric analysis and the percentages of these CD+ T cells were calculated. The ratio of regulatory T(Treg)/helper T 17(Th17) lymphocytes from 64 PD patients and 46 controls was determined by flow cytometric analysis.The results showed that the percentage of NK cells was higher in advanced PD patients than in controls(22.92% ±10.08% versus 19.76% ± 10.09%, P = 0.006), while CD3+ T cells are decreased(62.93% ± 9.27% versus65.75% ± 9.13%, P = 0.005). The percentage of CD19+B cells in male patients was lower(P = 0.021) than in female patients, whereas NK cells were increased(P \ 0.0001). The scores on the Unified Parkinson's Disease Rating Scale(UPDRS) and the Non-Motor Symptoms Scale in late-onset PD patients were significantly higher than those in earlyonset patients(P = 0.024 and P = 0.007, respectively). The percentage of CD19+ B cells in patients with UPDRS scores[24 was lower than in those with scores \24(10.17% ±4.19% versus 12.22% ± 5.39%, P = 0.009). In addition, the Treg/Th17 ratio in female patients was higher than that in female controls(13.88 ± 6.32 versus 9.94 ± 4.06, P =0.042). These results suggest that the percentages of NK cells,CD3+ T cells, and CD19+ B cells along with the Treg/Th17 ratio in peripheral blood may be used to predict the risk of PD in Chinese individuals and provide fresh avenues for novel diagnostic biomarkers and therapeutic designs. | Luan Cen Chaohao Yang Shuxuan Huang Miaomiao Zhou Xiaolu Tang Kaiping Li Wenyuan Guo Zhuohua Wu Mingshu Mo Yousheng Xiao Xiang Chen Xinling Yang Qinhui Huang Chaojun Chen Shaogang Qu Pingyi Xu | 2017 | Neuroscience Bulletin2017，33，5： | 5 |
| 10 | Transcriptome Profiling Reveals Auxin and Cytokinin Regulating Somatic Embryogenesis in Different Sister Lines of Cotton Cultivar CCRI24显示文摘在在棉花(Gossypium hirsutum L.) 位于体的 embryogenesis (SE ) 下面的分子的机制上得到一个更宽广的看法,棉花 transcriptome 动力学的全球分析用 RNA-Seq 在在不同姐妹线的 SE 期间被执行。204349 unigenes 的一个总数被 214977462 Illumina 的汇编读的 de novo 检测。量的反向的 transcriptionpolymerase 链反应(qRT-PCR ) 大小断然与几乎所有测试基因的 RNA-Seq 结果被相关(R2=0.841,关联在 0.01 水平是重要的) 。不同植物激素(植物生长素和细胞激动素) 在媒介和在在不同姐妹线的二个阶段的这二植物激素的内长的内容变化的集中比率在棉花 SE 期间建议了植物生长素和细胞激动素的角色。根据植物激素相关的基因的全球基因规定,从所有差别的众多的基因表示了抄本涉及植物生长素和细胞激动素生合成和信号 transduction 小径。差别的分析表示了涉及揭示的这些小径的基因在在二根姐妹线之间的基因类型和丰富的实质的变化。隔离,克隆并且在棉花 SE 期间揭示了显著起来表示或下面表示的基因是的 silencing/overexpressing 重要。而且,植物生长素和细胞激动素与对方或另外的因素遗体一起在 SE,而是潜在的串音起一个主要作用不清楚。 | Zhenzhen Xu Chaojun Zhang Xueyan Zhang Chuanliang Liu Zhixia Wu Zuoren Yang Kehai Zhou Xiaojie Yang Fuguang Li | 2013 | Journal of Integrative Plant Biology2013，55，7： | 5 |
| 11 | Structure and deformation measurements of shallow overburden during top coal caving longwall mining显示文摘Mining induced pressures are strong and overburden failure areas are large in top coal caving longwall mining, which constrains high production and safety mining. By employing the combination of the full view borehole photography technique and the seismic CT scanner technique, the deformation and failure of overlying strata of fully mechanized caving face in shallow coal seam were studied and the failure development of overburden was determined. Results show that the full view borehole photography can reveal the characteristics of strata, and the seismic CT scanner can reflect the characteristics of strata between the boreholes. The combined measurement technique can effectively determine the height of fractured and caved zones. The top end of the caved zone in Yangwangou coal mine employing the top coal caving longwall mining was at the depth of 171 m and fractured zone was at the depth of 106-110 m. The results provide a theoretic foundation for controlling the overburden strata in the shallow buried top coal caving panel. | Li Sheng Fan Chaojun Luo Mingkun Yang Zhenhua Lan Tianwei Zhang Haifeng | 2017 | International Journal of Mining Science and Technology2017，27，6： | 4 |
| 12 | A Superaerophobic Bimetallic Selenides Heterostructure for Efficient Industrial-Level Oxygen Evolution at Ultra-High Current Densities显示文摘Cost-effective and stable electrocatalysts with ultra-high current densities for electrochemical oxygen evolution reaction(OER)are critical to the energy crisis and environmental pollution.Herein,we report a superaerophobic three dimensional(3D)heterostructured nanowrinkles of bimetallic selenides consisting of crystalline NiSe2 and NiFe2Se4 grown on NiFe alloy(NiSe2/NiFe2Se4@NiFe)prepared by a thermal selenization procedure.In this unique 3D heterostructure,numerous nanowrinkles of NiSe2/NiFe2Se4 hybrid with a thickness of ~100 nm are grown on NiFe alloy in a uniform manner.Profiting by the large active surface area and high electronic conductivity,the superaerophobic NiSe2/NiFe2Se4@NiFe heterostructure exhibits excellent electrocatalytic activity and durability towards OER in alkaline media,outputting the low potentials of 1.53 and 1.54 V to achieve ultra-high current densities of 500 and 1000 mA cm^−2,respectively,which is among the most active Ni/Fe-based selenides,and even superior to the benchmark Ir/C catalyst.The in-situ derived FeOOH and NiOOH species from NiSe2/NiFe2Se4@NiFe are deemed to be efficient active sites for OER. | Jiaxin Yuan Xiaodi Cheng Hanqing Wang Chaojun Lei Sameer Pardiwala Bin Yang Zhongjian Li Qinghua Zhang Lecheng Lei Shaobin Wang Yang Hou | 2020 | Nano-Micro Letters2020，12，8： | 4 |
| 13 | Protein prenylation and human diseases: a balance of protein farnesylation and geranylgeranylation显示文摘The protein prenylation is one of the essential post-translational protein modifications, which extensively exists in the eukaryocyte. It includes protein farnesylation and geranylgeranylation, using farnesyl pyrophosphate(FPP) or geranylgeranyl pyrophosphate(GGPP) as the substrate, respectively. The prenylation occurs by covalent addition of these two types of isoprenoids to cysteine residues at or near the carboxyl terminus of the proteins that possess Caa X motif, such as Ras small GTPase family. The attachment of hydrophobic prenyl groups can anchor the proteins to intracellular membranes and trigger downstream cell signaling pathway. Geranylgeranyl biphosphate synthase(GGPPS) catalyzes the synthesis of 20-carbon GGPP from 15-carbon FPP. The abnormal expression of this enzyme will affect the relative content of FPP and GGPP, and thus disrupts the balance between protein farnesylation and geranylgeranylation, which participates into various aspects of cellular physiology and pathology. In this paper, we mainly review the property of this important protein post-translational modification and research progress in its regulation of cigarette smoke induced pulmonary disease, adipocyte insulin sensitivity, the inflammation response of Sertoli cells, the hepatic lipogenesis and the cardiac hypertrophy. | XU Na SHEN Ning WANG XiuXing JIANG Shan XUE Bin LI ChaoJun | 2015 | Science China(Life Sciences)2015，58，4： | 3 |
| 14 | Analysis of genetic alterations identifies the frequent mutation of GNAS in colorectal laterally spreading tumors显示文摘Dear Editor,Colorectal cancer(CRC)is among the most commonly diagnosed cancer and the leading cause of cancer-related death worldwide[1].Most CRCs develop from adenomas.Laterally spreading tumors(LSTs)are non-polypoid superficial colorectal neoplasms(CRNs)that are larger than 1 cm and typically extend laterally along the luminal wall.Based on surface morphology,it can be classified into a granular or nongranular type.The pathological morphology of LST manifests only as adenoma or with cancer.Recent studies have characterized 45-79%of LSTs as high-grade intraepithelial neoplasia(HGIN)or submucosal invasion;and the risk of submucosal invasion increases with lesion size[2].Hence,LST is considered a precancerous CRC lesion.Patients with LSTs are usually asymptomatic and diagnosis typically occurs during physical examinations or screening colonoscopy. | Lei Pan Junsheng Chen Chaojun Zhu Lu Han Aimin Li Side Liu | 2020 | Cancer Communications2020，40，11： | 3 |
| 15 | Scientific challenges of research on natural hazards and disaster risk显示文摘As a discipline,the science of natural hazards and disaster risk aims to explain the spatial-temporal pattern,process and mechanism,emergency response and risk mitigation of natural hazards,which requires a multidisci-plinary and interdisciplinary approach.With the support of Natural Science Finance of China(NSFC)and Chinese Academy of Sciences(CAS),in-depth research and systematic analysis on natural hazards and disaster risk were conducted.In this paper,the state of the art in research on natural hazards is summarized from seven aspects:formation process,mechanism and dynamic of natural hazards,disaster risk assessment,forecast,monitoring and early warning,disaster mitigation,emergency treatment and rescue,risk management and post-disaster re-construction.The trends within the natural hazards and disaster risk as a discipline were identified,along with existing shortcomings and significant gaps that need to be addressed.This paper highlighted:1)the scientific challenges including the frontier scientific issues and technological gaps on natural hazards and disaster risk dis-cipline from 2025 to 2035 in China,and 2)the proposal to develop a systemic and holistic natural hazards and disaster risk discipline. | Peng Cui Jianbing Peng Peijun Shi Huiming Tang Chaojun Ouyang Qiang Zou Lianyou Liu Changdong Li Yu Lei | 2021 | Geography and Sustainability2021，2，3： | 3 |
| 16 | Prediction of VIGS efficiency by the Sfold program and its reliability analysis in Gossypium hirsutum显示文摘Genetic transformation in some plant species,including cotton(Gossypium hirsutum), is hampered by laborious and time-consuming processes and often unachievable. Virus-induced gene silencing(VIGS) by double-stranded RNAs can serve as a reverse-genetics tool to determine gene function. However, knockdown levels vary greatly when using a tobacco rattle virus-based vector that carries different c DNA fragments of a gene. How to choose the optional target fragment for high interference efficiency is very challenging. Addressing this challenge requires increasing the efficacy of small interference RNA(si RNA) in target fragment. Here, we describe a method to assess VIGS efficiency by comparing the following parameters of si RNA in target sequence: the disruption energy of the target(DGdisruption), the differential stability of si RNA duplex ends(DSSE), and the internal stability at positions 9–14 of the si RNA antisense strand(AIS), which are calculated by Sfold program(http://gffzz01a2382fbe924301hpw6ouvkp65wk6wbv.ffgz.tsg.suse.edu.cn). We find that the si RNAs with low DGdisruption, high DSSE and high AIS have high activity and easily result in high VIGS efficiency by experimentally testing the actual knockdown levels of the four target genes, Gh PDS,Gh CLA1, Gh AOS1, and Gh CXE1 via choosing different target sequences for each gene. Therefore, the Sfold program can be used to analyze target sequences when carrying out VIGS design to increase gene-silencing effects in plants. | Xiaoyang Ge Jie Wu Chaojun Zhang Qianhua Wang Yuxia Hou Zuoren Yang Zhaoen Yang Zhenzhen Xu Ye Wang Lili Lu Xueyan Zhang Jinping Hua Fuguang Li | 2016 | Science Bulletin2016，61，7： | 2 |
| 17 | CYCLIC HARDENING BEHAVIOR OF POLYCRYSTALS WITH PENETRABLE GRAIN BOUNDARIES:TWO-DIMENSIONAL DISCRETE DISLOCATION DYNAMICS SIMULATION显示文摘由 Giessen 和裁缝( 1995 )的一种二维的分离脱臼动力学( DDD )技术,被集成一个脱臼谷物边界相互作用模型扩大了,习惯于计算联盟者分析包含微米大小的谷物的多晶体的微周期的塑料反应,与到多晶体和内在的脱臼机制的微塑料的周期的回答上的脱臼可穿透的谷物边界( GB )的重要影响的特殊注意。近这结束,在装载的简单紧张压缩下面的一个典型多晶的矩形的标本被考虑。结果显示出那,随积聚的紧张,不断的脱臼累积和提高的脱臼脱臼相互作用导致周期的变硬的周期的增加行为;然而,当在脱臼成核,通过 GB 的穿入和脱臼歼灭之中的动态平衡近似被建立时,周期的应力逐渐地趋于浸透。另外,包括谷物尺寸,周期的紧张振幅和它的历史,另外的因素也在周期的变硬和浸透上有可观的影响。 | Chuantao Hou Zhenhuan Li Minsheng Huang Chaojun Ouyang | 2009 | Acta Mechanica Solida Sinica2009，22，4： | 2 |
| 18 | Reflections on the Catastrophic 2020 Yangtze River Basin Flooding in Southern China显示文摘During the summer of 2020,southern China suffered from catastrophic flooding,the massive basin flood mainly occurred in the Yangtze River's middle and lower reaches.As of July 20,2020,the cumulative precipitation since the rain season started exceeded 500 mm over most of the Yangtze River Basin,reaching>1,200 mm in some areas of the lower reaches,exceeding the same period in 1998,the severest flood year in the past 60 years.Several water gauge stations at Poyang Lake and Chaohu Lake exceeded their historical record values.The water levels in the Wuhan section of the Yangtze River were among the highest ever recorded.As of July 22,2020,45.5 million people had been affected by the floods,with 142 people having died or were missing,and the direct economic losses expected to be around 116 billion RMB(~16.5 billion US dollars).However,the losses from this flood are much lower than those of previous major floods.For example,the catastrophic 1998 flooding led to 1,526 deaths and economic losses of 255 billion RMB(~36.4 billion US dollars).The reasons behind this are worthy of further study and discussion,and may be used for reference in the future and for other countries. | Ke Wei Chaojun Ouyang Hongtao Duan Yunliang Li Mingxing Chen Jiao Ma Huicong An Shu Zhou | 2020 | The Innovation2020，1，2： | 2 |
| 19 | GRP78 inhibits macrophage adhesion via SR-A显示文摘Class A scavenger receptor(SR-A)plays an important role in macrophage adhesion.However,the underlying mechanism remains unclear.We previously found that 78 kDa glucose-regulated protein(GRP78)inhibited SRA-mediated ligand internalization into macrophage by binding to SR-A.The aim of the study was to investigate whether GRP78 could regulate SR-A-mediated cell adhesion.We demonstrated that GRP78 bound directly to SR-A by fluorescence resonance energy transfer(FRET)assay.Overexpression of GRP78 inhibited macrophage adhesion via SR-A.These results suggest that GRP78 may act as an inhibitor of macrophage adhesion via SR-A. | Hui Bai Nan Li Xiaodan Zhou Chenchen Wang Yan Zhang Xudong Zhu Min Huang Yaoyu Chen Xiaoyu Li Qing Yang Chaojun Li Jingjing Ben Qi Chen | 2014 | The Journal of Biomedical Research2014，28，4： | 2 |
| 20 | A New Indicator for Global Food Security Assessment: Harvested Area Rather Than Cropland Area显示文摘Cropland area has long been used as a key indicator of food security.However,grain yield is not solely controlled by the area of the cropland.Therefore,we proposed a new indicator to assess food security.Results show that from 1992 to 2004,the global cropland area increased by 840200 km^(2)(99.4%),but the grain yield increased only by 310 million t(29.1%);and from 2004 to 2015,the cropland area decreased by 39000 km^(2)(4.64%),but the grain yield increased by 370 million t(70.84%).This result showed that grain yield was not linearly correlated with cropland area,and delimiting the threshold of cropland protection may not guarantee food security.Combined with further correlation analysis,we found that the increase in the global grain yield was more closely related to the harvested area(R^(2)=0.94),which indicated that the harvested area is a more scientific and accurate indicator than cropland area in terms of guaranteeing food security.Therefore,if governments want to ensure the food security,they should choose a new and more accurate indicator:harvested area rather than cropland area. | SONG Fengjiao WANG Shijie BAI Xiaoyong WU Luhua WANG Jinfeng LI Chaojun CHEN Huan LUO Xuling XI Huipeng ZHANG Sirui LUO Guofeng YAN Mengqi ZHEN Qingqing | 2022 | Chinese Geographical Science2022，32，2： | 2 |
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