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| 1 | Functional identification of the terpene synthase family involved in diterpenoid alkaloids biosynthesis in Aconitum carmichaelii显示文摘Aconitum carmichaelii is a high-value medicinal herb widely used across China,Japan,and other Asian countries.Aconitine-type diterpene alkaloids(DAs)are the characteristic compounds in Aconitum.Although six transcriptomes,based on short-read next generation sequencing technology,have been reported from the Aconitum species,the terpene synthase(TPS)corresponding to DAs biosynthesis remains unidentified.We apply a combination of Pacbio isoform sequencing and RNA sequencing to provide a comprehensive view of the A.carmichaelii transcriptome.Nineteen TPSs and five alternative splicing isoforms belonging to TPS-b,TPS-c,and TPS-e/f subfamilies were identified.In vitro enzyme reaction analysis functional identified two sesqui-TPSs and twelve di TPSs.Seven of the TPS-c subfamily genes reacted with GGPP to produce the intermediate ent-copalyl diphosphate.Five Ac KSLs separately reacted with ent-CPP to produce ent-kaurene,ent-atiserene,and ent-13-epi-sandaracopimaradie:a new diterpene found in Aconitum.Ac TPSs gene expression in conjunction DAs content analysis in different tissues validated that ent-CPP is the sole precursor to all DAs biosynthesis,with Ac KSL1,Ac KSL2 s and Ac KSL3-1 responsible for C20 atisine and napelline type DAs biosynthesis,respectively.These data clarified the molecular basis for the C20-DAs biosynthetic pathway in A.carmichaelii and pave the way for further exploration of C19-DAs biosynthesis in the Aconitum species. | Liuying Mao Baolong Jin Lingli Chen Mei Tian Rui Ma Biwei Yin Haiyan Zhang Juan Guo Jinfu Tang Tong Chen Changjiangsheng Lai Guanghong Cui Luqi Huang | 2021 | Acta Pharmaceutica Sinica B2021，11，10： | 6 |
| 2 | K-Ar dating of extensional fault gouge from the Yi-Shu segment of the Tan-Lu fault zone显示文摘Two grabens were developed in the Yi-Shu segment of the Tan-Lu fault zone (TLFZ) during its exten-sional activities, and are now confined by four major NNE-trending normal faults and filled with Creta-ceous sediments. These faults were developed due to their reactivities, containing gouge and cutting the graben sediments. Detailed fieldwork demonstrates that the faults experienced sinistral transten-sional moment related to regional NE-SW extension during the reactivity. X-ray diffraction (XRD) analysis of the finest gouge samples gives illite crystallinity values higher than 0.42o?2θ, indicating temperatures experienced by the gouge samples were less than 150℃ . From the relation between K-Ar data and proportions of detrital illite in different size fractions of the gouge samples, we conclude that refaulting for the western boundary fault of the TLFZ, abbreviated to F4, took place at ca. 90 Ma and for the eastern boundary fault, abbreviated to F1, happened from 70 to 60 Ma. During the two phases of reactivity imposed by the same NE-SW extension, the TLFZ experienced uplifting and no sediments were deposited in the two grabens. It is suggested that the TLFZ experienced extension during the Late Cretaceous, which supports the inference that lithospheric thinning was still undergoing in the east of the North China Craton during the Late Cretaceous magmatic hiatus. | WANG YongSheng ZHU Guang HU ZhaoQi ZHANG BiLong XIANG BiWei XIE ChengLong | 2009 | Science China Earth Sciences2009，52，4： | 5 |
| 3 | Progress in the studies on the molecular mechanisms associated with multidrug resistance in cancers显示文摘Chemotherapy is one of the important methods to treat cancer,and the emergence of multidrug resistance(MDR)is one major cause for the failure of cancer chemotherapy.Almost all anti-tumor drugs develop drug resistance over a period of time of application in cancer patients,reducing their effects on killing cancer cells.Chemoresistance can lead to a rapid recurrence of cancers and ultimately patient death.MDR may be induced by multiple mechanisms,which are associated with a complex process of multiple genes,factors,pathways,and multiple steps,and today the MDR-associated mechanisms are largely unknown.In this paper,from the aspects of protein–protein interactions,alternative splicing(AS)in pre-mRNA,non-coding RNA(ncRNA)mediation,genome mutations,variance in cell functions,and influence from the tumor microenvironment,we summarize the molecular mechanisms associated with MDR in cancers.In the end,prospects for the exploration of antitumor drugs that can reverse MDR are briefly discussed from the angle of drug systems with improved targeting properties,biocompatibility,availability,and other advantages. | Lei Zhang Biwei Ye Zhuo Chen Zhe-Sheng Chen | 2023 | Acta Pharmaceutica Sinica B2023，13，3： | 3 |
| 4 | Spleen-kidney supplementing formula alleviates insulin resistance via regulating AKT/glycogen synthase kinase 3β pathway in rats with type 2 diabetic induced by high-fat diet显示文摘OBJECTIVE: To explore the molecular mechanism underpinning the action by investigating its effect on glycogen content and AKT(also known as protein kinase B)/glycogen synthase kinase 3β(GSK-3β) pathway in the liver of rats with type 2 diabetic induced by high-fat diet.METHODS: The rat model of type 2 diabetes was induced by high-fat diet and multiple low-dose streptozotocin injection. Diabetic rats were divided into five groups: the model control group, the Metformin group, spleen-kidney supplementing formula groups of low, medium and high doses. Fasting blood glucose(FBG) levels were measured before treatment and every two weeks during treatment.After the treatment, oral glucose tolerance test was performed, and hemoglobin A1c (HbA1c) and C-peptide were measured to assess the formula's effect on glucose metabolism and insulin resistance. The protein expression levels of AKT, GSK-3βand their phosphorylated forms in the liver were also measured to study the formula's role in insulin signaling pathway.RESULTS: Spleen-kidney supplementing formula significantly relieved the symptoms of polydipsia,polyuria and weight loss in type 2 diabetic rats, reduced FBG and HbA1c levels, increased glycogen content, and improved insulin sensitivity. The anti-diabetic effects of spleen-kidney supplementing formula are dose dependent. It also increased the total AKT protein level and the GSK-3β phosphorylation in the liver of type 2 diabetic rats.CONCLUSION: Spleen-kidney supplementing formula has hypoglycemic effect and relieves insulin resistance by enhancing AKT/GSK-3β signaling pathway in the liver of type 2 diabetic rats. | Tian Chunyu Wang Ya La Xiaojin Li Ji'an Zhang Biwei | 2019 | Journal of Traditional Chinese Medicine2019，39，2： | 2 |
| 5 | A Dynamic and Deadline-Oriented Road Pricing Mechanism for Urban Traffic Management显示文摘Road pricing is an urban traffic management mechanism to reduce traffic congestion.Currently,most of the road pricing systems based on predefined charging tolls fail to consider the dynamics of urban traffic flows and travelers’demands on the arrival time.In this paper,we propose a method to dynamically adjust online road toll based on traffic conditions and travelers’demands to resolve the above-mentioned problems.The method,based on deep reinforcement learning,automatically allocates the optimal toll for each road during peak hours and guides vehicles to roads with lower toll charges.Moreover,it further considers travelers’demands to ensure that more vehicles arrive at their destinations before their estimated arrival time.Our method can increase the traffic volume effectively,as compared to the existing static mechanisms. | Jiahui Jin Xiaoxuan Zhu Biwei Wu Jinghui Zhang Yuxiang Wang | 2022 | Tsinghua Science and Technology2022，27，1： | 1 |
| 6 | A novel strategy for boosting the photoluminescence quantum efficiency of CdSe nanocrystals at room temperature显示文摘Highly luminescent colloidal nanocrystals have wide applications in bioimaging and various optoelectronic devices.Herein we report a facile and mild procedure by combining S2-treatment and binary ligand passivation,which can efficiently enhance the luminescent property of CdSe nanocrystals at room temperature.The photoluminescence quantum yield of as-treated CdSe nanocrystals exhibits drastic enhancement(e.g.,188 times for CdSe nanorods)after this dual-passivation treatment.The methodology proposed here can be applied to various CdSe nanocrystals,regardless of their sizes,shapes,and crystal structures. | Biwei Wang Limin Liu Yi Zhang Yonghui Deng Angang Dong | 2020 | Chinese Chemical Letters2020，31，1： | 1 |
| 7 | Multidither coherent optical adaptive technique for deep tissue two-photon microscopy显示文摘Two-photon microscopy normally suffers from the scattering of the tissue in biological imaging.Multidither coberent optical adaptive technique(COAT)can correct the scattered wavefront in parallel.However,the determination of the corrective phases may not be completely accurate using conventional method,which undermines the performance of this technique.In this paper,we theoretically demonstrate a method that can obtain more accurate corrective phases by determining the phase values from the square root of the fuorescence signal.A numnerical simulation model is established to study the performance of adaptive optics in two-photon micros-copy by combining scalar diffraction theory with vector diffraction theory.The results show that the distortion of the wavefront can be corrected more thoroughly with our method in two-photon imaging.In our simulation,with the scattering from a 450-mn-thick mouse brain tissue,excitation focal spots with higher peak-to background ratio(PBR)and images with higher contrast can be obtained.Hence,further enhancement of the multidither COAT correction performance in two-photon imaging can be expected. | Biwei Zhang Wei Gong Chenxue Wu Lejia Hu Xinpei Zhu Ke Si | 2019 | Journal of Innovative Optical Health Sciences2019，，4： | 0 |
| 8 | Challenges and approaches of single-crystal Ni-rich layered cathodes in lithium batteries显示文摘High energy density and high safety are incompatible with each other in a lithium battery,which challenges today's energy storage and power applications.Ni-rich layered transition metal oxides(NMCs)have been identified as the primary cathode candidate for powering next-generation electric vehicles and have been extensively studied in the last two decades,leading to the fast growth of their market share,including both polycrystalline and single-crystal NMC cathodes.Single-crystal NMCs appear to be superior to polycrystalline NMCs,especially at low Ni content(≤60%).However,Ni-rich single-crystal NMC cathodes experience even faster capacity decay than polycrystalline NMC cathodes,rendering them unsuitable for practical application.Accordingly,this work will systematically review the attenuation mechanism of single-crystal NMCs and generate fresh insights into valuable research pathways.This perspective will provide a direction for the development of Ni-rich single-crystal NMC cathodes. | Jiangtao Hu Hongbin Wang Biwei Xiao Pei Liu Tao Huang Yongliang Li Xiangzhong Ren Qianling Zhang Jianhong Liu Xiaoping Ouyang Xueliang Sun | 2023 | National Science Review2023，10，12： | 0 |
| 9 | Hollow CdS-based photocatalysts显示文摘In recent years,photocatalytic technology,driven by solar energy,has been extensively investigated to ease energy crisis and environmental pollution.Nevertheless,efficiency and stability of photocatalysts are still unsatisfactory.To address these issues,design of advanced photocatalysts is important.Cadmium sulphide(CdS)nanomaterials are one of the promising photocatalysts.Among them,hollow-structured CdS,featured with enhanced light absorption ability,large surface area,abundant active sites for redox reactions,and reduced diffusion distance of photogenerated carriers,reveals a broad application prospect.Herein,main synthetic strategies and formation mechanism of hollow CdS photocatalysts are summarized.Besides,we comprehensively discuss the current development of hollow-structured CdS nanomaterials in photocatalytic applications,including H2 production,CO2 reduction and pollutant degradation.Finally,brief conclusions and perspectives on the challenges and future directions for hollow CdS photocatalysts are proposed. | Xiangyu Liu Mahmoud Sayed Chuanbiao Bie Bei Cheng Biwei Hu Jiaguo Yu Liuyang Zhang | 2021 | Journal of Materiomics2021，7，3： | 0 |
| 10 | Comprehensive Metabolic Profiling and Genome-wide Analysis Reveal Therapeutic Modalities for Hepatocellular Carcinoma显示文摘Understanding the details of metabolic reprogramming in hepatocellular carcinoma(HCC)is critical to improve stratification for therapy.Both multiomics analysis and cross-cohort validation were performed to investigate the metabolic dysregulation of 562 HCC patients from 4 cohorts.On the basis of the identified dynamic network biomarkers,227 substantial metabolic genes were identified and a total of 343 HCC patients were classified into 4 heterogeneous metabolic clusters with distinct metabolic characteristics:cluster 1,the pyruvate subtype,associated with upregulated pyruvate metabolism;cluster 2,the amino acid subtype,with dysregulated amino acid metabolism as the reference;cluster 3,the mixed subtype,in which lipid metabolism,amino acid metabolism,and glycan metabolism are dysregulated;and cluster 4,the glycolytic subtype,associated with the dysregulated carbohydrate metabolism.These 4 clusters showed distinct prognoses,clinical characteristics and immune cell infiltrations,which was further validated by genomic alterations,transcriptomics,metabolomics,and immune cell profiles in the other 3 independent cohorts.Besides,the sensitivity of different clusters to metabolic inhibitors varied depending on their metabolic features.Importantly,cluster 2 is rich in immune cells in tumor tissues,especially programmed cell death protein 1(PD-1)-expressing cells,which may be due to the tryptophan metabolism disorders,and potentially benefiting more from PD-1 treatment.In conclusion,our results suggest the metabolic heterogeneity of HCC and make it possible to treat HCC patients precisely and effectively on specific metabolic characteristics. | Feng Qi Jia Li Zhuoran Qi Jian Zhang Bin Zhou Biwei Yang Wenxing Qin Wenguo Cui Jinglin Xia | 2023 | Research2023，，3： | 0 |
| 11 | Aerobic Oxidation of 2,3,6-Trimethylphenol with Reusable Homogenized Copper Catalysts显示文摘The oxidation of 2,3,6-trimethylphenol(TMP)can produce both 2,3,5-trimethyl-1,4-benzoquinone(TMQ)and 2,3,5-trimethyl-1,4-hydroquinone(TMHQ)as important intermediates of vitamin E.With a soluble and porous polymer of intrinsic microporosity(PIM-1),we developed a highly active and reusable CuO@PIM-1 catalyst for the aerobic oxidation of TMP to TMQ and TMHQ.The CuO@PIM-1 catalyst is high active and selective for the aerobic oxidation of TMP,and TMQ and TMHQ can be obtained in yields of 81%and 19%,respectively.The CuO@PIM-1 catalyst has both advantages of homogeneous and heterogeneous and the CuO@PIM-1 catalyst showed a higher activity than homogeneous(CuCl_(2))and heterogeneous(supported CuO)catalysts.Moreover,CuO@PIM-1 catalyst can be recovered easily and reused at least 5 times without deactivation. | LI Meng WU Mingwei PAN Biwei XU Dandan LVSONG Zhuopu LI Dongqing ZHANG Fangrong HUANG Jun | 2021 | Chemical Research in Chinese Universities2021，37，3： | 0 |
| 12 | The infrared spectral features of circumstellar envelope of evolved low- and intermediate-mass stars显示文摘The circumstellar envelope of evolved stars of low- and intermediate-mass is an important site for dust formation. In comparison with the interstellar medium, they have more types of organics and different types of inorganics. Various infrared features in the circumstellar envelope can reveal the composition and abundance of dust, as well as the chemical and physical conditions of the circumstellar shell. Infrared features and their carriers are different in the C-rich or O-rich environment, and the mixed-environment where the C-rich and O-rich circumstellar materials co-exist. The C-rich sources exhibit a series of spectral features which are attrib- uted to organic molecules. They also show two prominent features at 21 μm and 30 μm which emit a large portion of infrared radiation. The O-rich sources exhibit the strong 9.7 μm and 18 μm features attributed to the Si-O bending and O-Si-O stretching modes of amorphous silicate dust. With the ISO/SWS spectrometer, about 50 narrow bands are identified with the crystalline silicate grains, mainly forsterite and enstatite. In addition, a series of features, at 13 μm, 16.8 μm, 19.5 μm and 31.8 μm, appearing to be correlated with each other, are attributed to oxides. Some objects simultaneously show the C-rich and O-rich features, e.g. some C-rich sources have silicate features. There is no well-accepted interpretation for such mixed appearance, though a binary model is suggested. | ZHANG Ke JIANG BiWei | 2008 | Science China(Physics,Mechanics & Astronomy)2008，51，9： | 0 |
| 13 | Calcyclin-binding protein contributes to cholangiocarcinoma progression by inhibiting ubiquitination of MCM2显示文摘Background:Cholangiocarcinoma(CCA)represents the epithelial cell cancer with high aggressiveness whose five-year survival rate is poor with standard treatment.Calcyclin-binding protein(CACYBP)shows aberrant expression within several malignant tumors,but the role of CACYBP in CCA remains unknown.Methods:Immunohistochemical(IHC)analysis was used to identify CACYBP overexpression in clinical samples of CCA patients.Moreover,its correlation with clinical outcome was revealed.Furthermore,CACYBP’s effect on CCA cell growth and invasion was investigated in vitro and in vivo using loss-of-function experiments.Results:CACYBP showed up-regulation in CCA,which predicts the dismal prognostic outcome.CACYBP had an important effect on in-vitro and in-vivo cancer cell proliferation and migration.Additionally,knockdown of CACYBP weakened protein stability by promoting ubiquitination of MCM2.Accordingly,MCM2 up-regulation partly reversed CACYBP deficiency’s inhibition against cancer cell viability and invasion.Thus,MCM2 might drive CCA development by Wnt/β-catenin pathway.Conclusions:CACYBP exerted a tumor-promoting role in CCA by suppressing ubiquitination of MCM2 and activating Wnt/β-catenin pathway,hence revealing that it may be the possible therapeutic target for CCA treatment. | YUSEN ZHANG LIPING LIU BIWEI LUO HONGGUI TANG XIAOFANG YU SHIYUN BAO | 2023 | Oncology Research2023，31，3： | 0 |
| 14 | A Meta-analysis of Acupuncture Treatment for Irritable Bowel Syndrome显示文摘To assess the efficacy of acupuncture for treatment of irritable bowel syndrome through meta-analysis of randomized controlled trials in recent 20 years.Online databases,including CNKI,VIP,WANFANG,PubMed,Cochrane Library,Web of Science and Embase were searched for randomized controlled trials(RCTs)of acupuncture for IBS.Retrieval time was from January 1,2000 to January 31,2021.According to Jadad scoring criteria,the bias risk and quality assessment of each RCT included were evaluated by two researchers.RevMan 5.3 software was used for the metaanalysis.Eight RCTs were selected which include a total of 1181 patients.The control group has 425 patients and the experimental group has 756 patients.The result of meta-analysis indicates that the total effective rate for the experimental group was superior to that of the control group[OR=3.29,95%CI[2.16~5.03](P<0.01)],and the funnel plot was basically symmetric.Acupuncture therapy is shown to have a good safety and compliance record.However,the number of high-quality trials is small,and there are some deficiencies in the methodology of clinical research.Acupuncture,as a supplementary therapy for irritable bowel syndrome,has positive clinical significance and prospects for application.The methodology of clinical research needs to be further improved. | Biwei Chen Lili Zhang Shaozong Chen Changzhen Gong | 2021 | Journal of Integrative Medicine(双语)2021，10，2： | 0 |
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