| 1 | Functional identification of the terpene synthase family involved in diterpenoid alkaloids biosynthesis in Aconitum carmichaelii显示文摘Aconitum carmichaelii is a high-value medicinal herb widely used across China,Japan,and other Asian countries.Aconitine-type diterpene alkaloids(DAs)are the characteristic compounds in Aconitum.Although six transcriptomes,based on short-read next generation sequencing technology,have been reported from the Aconitum species,the terpene synthase(TPS)corresponding to DAs biosynthesis remains unidentified.We apply a combination of Pacbio isoform sequencing and RNA sequencing to provide a comprehensive view of the A.carmichaelii transcriptome.Nineteen TPSs and five alternative splicing isoforms belonging to TPS-b,TPS-c,and TPS-e/f subfamilies were identified.In vitro enzyme reaction analysis functional identified two sesqui-TPSs and twelve di TPSs.Seven of the TPS-c subfamily genes reacted with GGPP to produce the intermediate ent-copalyl diphosphate.Five Ac KSLs separately reacted with ent-CPP to produce ent-kaurene,ent-atiserene,and ent-13-epi-sandaracopimaradie:a new diterpene found in Aconitum.Ac TPSs gene expression in conjunction DAs content analysis in different tissues validated that ent-CPP is the sole precursor to all DAs biosynthesis,with Ac KSL1,Ac KSL2 s and Ac KSL3-1 responsible for C20 atisine and napelline type DAs biosynthesis,respectively.These data clarified the molecular basis for the C20-DAs biosynthetic pathway in A.carmichaelii and pave the way for further exploration of C19-DAs biosynthesis in the Aconitum species. | Liuying Mao Baolong Jin Lingli Chen Mei Tian Rui Ma Biwei Yin Haiyan Zhang Juan Guo Jinfu Tang Tong Chen Changjiangsheng Lai Guanghong Cui Luqi Huang | 2021 | Acta Pharmaceutica Sinica B2021，11，10： | 6 |
| 2 | 3D cellular-resolution imaging in arteries using few-mode interferometry显示文摘Cross-sectional visualisation of the cellular and subcellular structures of human atherosclerosis in vivo is significant,as this disease is fundamentally caused by abnormal processes that occur at this scale in a depth-dependent manner.However,due to the inherent resolution-depth of focus tradeoff of conventional focusing optics,today’s highestresolution intravascular imaging technique,namely,optical coherence tomography(OCT),is unable to provide crosssectional images at this resolution through a coronary catheter.Here,we introduce an intravascular imaging system and catheter based on few-mode interferometry,which overcomes the depth of focus limitation of conventional highnumerical-aperture objectives and enables three-dimensional cellular-resolution intravascular imaging in vivo by a submillimetre diameter,flexible catheter.Images of diseased cadaver human coronary arteries and living rabbit arteries were acquired with this device,showing clearly resolved cellular and subcellular structures within the artery wall,such as individual crystals,smooth muscle cells,and inflammatory cells.The capability of this technology to enable cellularresolution,cross-sectional intravascular imaging will make it possible to study and diagnose human coronary disease with much greater precision in the future. | Biwei Yin Zhonglie Piao Kensuke Nishimiya Chulho Hyun Joseph A.Gardecki Adam Mauskapf Farouc A.Jaffer Guillermo J.Tearney | 2019 | Light(Science & Applications)2019，8，1： | 0 |
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