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| 1 | A Kelch Motif-Containing Serine/Threonine Protein Phosphatase Determines the Large Grain QTL Trait in Rice显示文摘A thorough understanding of the genetic basis of rice grain traits is critical for the improvement of rice (Oryza sativa L.) varieties.In this study,we generated an F 2 population by crossing the large-grain japonica cultivar CW23 with Peiai 64 (PA64),an elite indica small-grain cultivar.Using QTL analysis,17 QTLs for five grain traits were detected on four different chromosomes.Eight of the QTLs were newly-identified in this study.In particular,qGL3-1,a newly-identified grain length QTL with the highest LOD value and largest phenotypic variation,was fine-mapped to the 17 kb region of chromosome 3.A serine/threonine protein phosphatase gene encoding a repeat domain containing two Kelch motifs was identified as the unique candidate gene corresponding to this QTL.A comparison of PA64 and CW23 sequences revealed a single nucleotide substitution (C→A) at position 1092 in exon 10,resulting in replacement of Asp (D) in PA64 with Glu (E) in CW23 for the 364th amino acid.This variation is located at the D position of the conserved sequence motif AVLDT of the Kelch repeat.Genetic analysis of a near-isogenic line (NIL) for qGL3-1 revealed that the allele qGL3-1 from CW23 has an additive or partly dominant effect,and is suitable for use in molecular marker-assisted selection. | Zejun Hu Haohua He Shiyong Zhang Fan Sun Xiaoyun Xin Wenxiang Wang Xi Qian Jingshui Yang Xiaojin Luo | 2012 | Journal of Integrative Plant Biology2012，54，12： | 41 |
| 2 | A Novel QTL qTGW3 Encodes the GSK3/ SHAGGY-Like Kinase OsGSK5/OsSK41 that Interacts with OsARF4 to Negatively Regulate Grain Size and Weight in Rice显示文摘谷物尺寸和形状在米饭是谷物重量和产量的重要决定因素。这里,我们报导一个新主要量的特点在米饭的地点(QTL ) , qTGW3,那种控制谷物尺寸和重量。这个地点, qTGW3,编码 OsSK41 (也作为 OsGSK5 知道) , GLYCOGEN SYNTHASE KINASE 3/SHAGGY-like 家庭的一个成员。带 OsSK41 的 loss-of-function 等位基因的瑞斯 near-isogenic 线增加了谷物长度和重量。我们证明那 OsSK41 交往与并且 phosphorylates 植物生长素反应因素 4 (OsARF4 ) 。有 OsARF4 的 OsSK41 的合作表示在米饭原物增加 OsARF4 的累积。OsARF4 的功能的损失导致更大的米饭谷物。定序 RNA 分析建议 OsARF4 和 OsSK41 镇压下游的基因的一个普通集合的表示, ? 包括一些植物生长素应答的基因,在米饭谷物开发期间。在 qTGW3 的 OsSK41 的 loss-of-function 形式代表没被指向的基因编辑或 QTL 节节上升广泛地在 OsSK41 功能的米饭 breeding.?Suppression 利用了的稀罕等位基因提高米饭谷物尺寸和重量。因此,我们的学习在米饭谷物开发揭示 OsSK41 的重要角色 ?? 并且在另外的谷物庄稼在米饭并且也许为谷物产量的基因改进提供新候选人基因。 | Zejun Hu Sun-Jie Lu Mei-Jing Wang Haohua He Le Sun Hongru Wang Xue-Huan Liu Ling Jiang Jing-Liang sun Xiaoyun Xin Wei Kong Chengcai Chu Hong-Wei Xue Jinshui Yang Xiaojin Luo Jian-Xiang Liu | 2018 | Molecular Plant2018，11，5： | 47 |
| 3 | Nasal delivery of broadly neutralizing antibodies protects mice from lethal challenge with SARS-CoV-2 delta and omicron variants显示文摘Multiple new variants of severe acute respiratory syndrome coronavirus 2(SARS-Co V-2)have constantly emerged,as the delta and omicron variants,which have developed resistance to currently gained neutralizing antibodies.This highlights a critical need to discover new therapeutic agents to overcome the variants mutations.Despite the availability of vaccines against coronavirus disease 2019(COVID-19),the use of broadly neutralizing antibodies has been considered as an alternative way for the prevention or treatment of SARS-Co V-2 variants infection.Here,we show that the nasal delivery of two previously characterized broadly neutralizing antibodies(F61 and H121)protected K18-h ACE2 mice against lethal challenge with SARS-Co V-2 variants.The broadly protective efficacy of the F61 or F61/F121 cocktail antibodies was evaluated by lethal challenge with the wild strain(WIV04)and multiple variants,including beta(B.1.351),delta(B.1.617.2),and omicron(B.1.1.529)at 200or 1000 TCID_(50),and the minimum antibody administration doses(5-1.25 mg/kg body weight)were also evaluated with delta and omicron challenge.Fully prophylactic protections were found in all challenged groups with both F61 and F61/H121 combination at the administration dose of 20 mg/kg body weight,and corresponding mice lung viral RNA showed negative,with almost all alveolar septa and cavities remaining normal.Furthermore,low-dose antibody treatment induced significant prophylactic protection against lethal challenge with delta and omicron variants,whereas the F61/H121 combination showed excellent results against omicron infection.Our findings indicated the potential use of broadly neutralizing monoclonal antibodies as prophylactic and therapeutic agent for protection of current emerged SARS-Co V-2 variants infection. | Jia Lu Qiangling Yin Rongjuan Pei Qiu Zhang Yuanyuan Qu Yongbing Pan Lina Sun Ding Gao Cuiqin Liang Jingwen Yang Wei Wu Jiandong Li Zongqiang Cui Zejun Wang Xinguo Li Dexin Li Shiwen Wang Kai Duan Wuxiang Guan Mifang Liang Xiaoming Yang | 2022 | Virologica Sinica2022，37，2： | 4 |
| 4 | Generation and characterization of monoclonal antibodies against the NS1 protein of H17N10 bat influenza virus显示文摘 | Junna Gao Kaidi Xu Haiwei Sun Qinfang Liu Jianmei Yang Qiaoyang Teng Xuesong Li Zhaoguo Chen Zejun Li Hongjun Chen | 2017 | Acta Biochimica et Biophysica Sinica2017，49，7： | 2 |
| 5 | Chinese Antarctic Magnetometer Chain at the Cusp Latitude显示文摘A Chinese Antarctic Magnetometer(CAM) chain from Zhongshan Station(ZHS) to Dome-A(DMA) has been established since February 2009.A regular magnetometer is operated at ZHS,and four low power magnetometers are operated along the interior route from ZHS to DMA in the cusp latitude,extending over a distance of 1260 km.These stations fill an important void in the Antarctic magnetometer network.Furthermore,the CAM chain is magnetically conjugated with the Arctic region reaching from the Svalbard archipelago to Daneborg,on the east coast of Greenland.Conjugate measurements using the Arctic and Antarctic magnetometers provide excellent opportunities to investigate phenomena related to the coupling of the solar wind to the magnetosphere and ionosphere,such as magnetic impulse events,flux transfer events,traveling convection vortices and ultra-low frequency waves. | LIU Yonghua HU Hongqiao YANG Huigen ZHANG Beichen SUN Bo WEI Fuhai LIU Yang LIU Jianjun WANG Rui CHEN Zhuotian HU Zejun HAN Desheng SHI Guitao HU Zhengyi WANG Tao AN Chunlei Mike Rose | 2016 | Advances in Polar Science2016，27，2： | 2 |
| 6 | Scattered seeding of CAR T cells in solid tumors augments anticancer efficacy显示文摘Chimeric antigen receptor T cell(CAR T) therapy was a milestone in the treatment of relapsed and refractory B cell malignancies. However, beneficial effects of CAR T cells have not been obtained in solid tumors yet. Herein, we implement a porous microneedle patch that accommodates CAR T cells and allows in situ penetration-mediated seeding of CAR T cells when implanted in the tumor bed or in the post-surgical resection cavity. CAR T cells loaded in the pores of the microneedle tips were readily escorted to the tumor in an evenly scattered manner without losing their activity. Such microneedle-mediated local delivery enhanced infiltration and immunostimulation of CAR T cells as compared to direct intratumoral injection. This tailorable patch offers a transformative platform for scattered seeding of living cells for treating a variety of tumors. | Hongjun Li Zejun Wang Edikan A.Ogunnaike Qing Wu Guojun Chen Quanyin Hu Tianyuan Ci Zhaowei Chen Jinqiang Wang Di Wen Hongwei Du Jie Jiang Jie Sun Xingcai Zhang Gianpietro Dotti Zhen Gu | 2022 | National Science Review2022，9，3： | 2 |
| 7 | Subcellular Localization of Large Yellow Croaker(Larimichthys crocea) TLR21 and Expression Profiling of Its Gene in Immune Response显示文摘Toll-like receptor 21(TLR21) is a non-mammalian type TLR, and plays an important role in innate immune response in fish. In this paper, the full-length cDNA sequence of TLR21 gene was identified and characterized from large yellow croaker, Larimichthys crocea and was termed as LcTLR21. It consists of 3365 bp, including a 5'-terminal untranslated region(UTR) of 97 bp, a 3'-terminal UTR of 331 bp, and an open reading frame(ORF) of 2937 bp encoding a polypeptide of 978 amino acid residues. The deduced LcTLR21 contains a signal peptide domain at N-terminal, 12 leucine-rich repeats(LRRs) at the extracellular region, a transmembrane domain and a cytoplasmic toll-interleukin-1 receptor(TIR) domain at the C-terminal. Subcellular localization analysis revealed that the LcTLR21-GFP was constitutively expressed in cytoplasm. Tissue expression analysis indicated that LcTLR21 gene broadly expressed in most of the examined tissues, with the most predominant abundance in spleen, followed by head-kidney and liver, while the weakest expression was detected in brain. The expression level of LcTLR21 after LPS, poly I:C and Vibrio parahaemolyticus challenges was investigated in spleen, head-kidney and liver. LcTLR21 gene transcripts increased significantly in all examined tissues after the challenges, and the highest expression level was detected in liver at 24 h after poly I:C stimulation(P <0.05), suggesting that LcTLR21 might play a crucial role in fish resistance to viral and bacterial infections. | SUN Qingxue FAN Zejun YAO Cuiluan | 2018 | Journal of Ocean University of China2018，17，2： | 1 |
| 8 | High capacity adsorption of antimony in biomass-based composite and its consequential utilization as battery anode显示文摘Antimony is more than an emerging pollutant in water but a scare resource.In this study,we report an adsorbent with the record capacity so far from the balanced view of Sb(Ⅲ) and Sb(Ⅴ).The composite adsorbent was fabricated by encapsulating hollow Fe_(3)O_(4)nanosphere with the EDTA grafted chitosan,and it has superhigh adsorption capacity of for 657.1 mg/g for Sb(Ⅲ) and 467.3 mg/g for Sb(Ⅴ),respectively.The mechanism study reveals that the adsorption of Sb initializes from the Fe_(3)O_(4),propagates along the chitosan with hydrogen bond,and terminates at the inner sphere complex with the EDTA moiety in the adsorbent.In view of the ultra-high adsorption capacity of the adsorbent,the recovered adsorbent that contains abundant (>36.4%) highly dispersed antimony nanoparticles (600-FCSE-Sb) is applied to Li-ion battery anode after reduction.This article provides a new idea for connecting water treatment and electric energy storage. | Yao Wang Xinyue Zhang Na Ju Hongna Jia Zejun Sun Jiaxing Liang Rongxiu Guo Dun Niu Hong-bin Sun | 2023 | Journal of Environmental Sciences2023，，4： | 1 |
| 9 | Temporal and spatial stability of the EM/PM molecular subtypes in adult diffuse glioma显示文摘Detailed characterizations of genomic alterations have not identified subtype-specific vulnerabilities in adult gliomas. Mapping gliomas into developmental programs may uncover new vulnerabilities that are not strictly related to genomic alterations. After identifying conserved gene modules co-expressed with EGFR or PDGFRA (EM or PM), we recently proposed an EM/PM classification scheme for adult gliomas in a histological subtype- and grade-independent manner. By using cohorts of bulk samples, paired primary and recurrent samples, multi-region samples from the same glioma, single-cell RNA-seq samples, and clinical samples, we here demonstrate the temporal and spatial stability of the EM and PM subtypes. The EM and PM subtypes, which progress in a subtype-specific mode, are robustly maintained in paired longitudinal samples. Elevated activities of cell proliferation, genomic instability and microenvironment, rather than subtype switching, mark recurrent gliomas. Within individual gliomas, the EM/PM subtype was preserved across regions and single cells. Malignant cells in the EM and PM gliomas were correlated to neural stem cell and oligodendrocyte progenitor cell compartment, respectively. Thus, while genetic makeup may change during progression and/or within different tumor areas, adult gliomas evolve within a neurodevelopmental framework of the EM and PM molecular subtypes. The dysregulated developmental pathways embedded in these molecular subtypes may contain subtype-specific vulnerabilities. | Jing Feng Zheng Zhao Yanfei Wei Zhaoshi Bao Wei Zhang Fan Wu Guanzhang Li Zhiyan Sun Yanli Tan Jiuyi Li Yunqiu Zhang Zejun Duan Xueling Qi Kai Yu Zhengmin Cong Junjie Yang Yaxin Wang Yingyu Sun Fuchou Tang Xiaodong Su Chuan Fang Tao Jiang Xiaolong Fan | 2023 | Frontiers of Medicine2023，17，2： | 0 |
| 10 | Organophosphine ligand derived sandwich-structural electrocatalyst for oxygen evolution reaction显示文摘The synchronous construction of metal phosphate and phosphorus-doped carbon structures is of great significance to innovate the design,synthesis,and application of catalysts,as these phosphoruscontaining composite materials have shown a remarkable contribution to electrocatalysts.However,their preparation procedure generally involves using large amounts of excess phosphorus sources for phosphorization,which inevitably release poisonous PH_(3) or dangerous phosphorus vapor.Here,a strategy for in-situ formation of FePO_(4) embedded in P-doped carbon 2D nano film(FePO_(4)/PdC)is developed using a highly integrated precursor,which is a small molecular organophosphine ligand,1,1’bis(diphenylphosphine)ferrocene(DPPF).The multi-source precursor DPPF that contains Fe,P,and C is molecular-vapor-deposited on the nickel foam(NF)supported ZIF-67 nanosheets to obtain the composite catalyst,namely DPPF-500/ZIF-67/NF.FePO_(4)/PdC encapsulated the ZIF-67 derived Co/N-doped carbon matrix(Co NC)to form a sandwich structure FePO_(4)/PdC@CoNC.The constructed catalyst shows good performance for OER,requiring an overpotential of only 297 m V to deliver 600 m A/cm^(2) with a Tafel slope of 42.7 m V dec^(-1).DFT calculations demonstrate that the synergistic effects between the metal active center and P-doped carbon film reduce the energy barriers and improve electron transport.This method of constructing P-containing catalysts overcomes the demand for additional P sources to realize eco-friendly fabrication and yields a unique structure with good catalytic activity. | Zejun Sun Wenjuan Xu Liutao Guo Qiang Han Jianyi Gao Jiaping Wang Yanru Feng Chengrui Li Qionglin Liang Hong-bin Sun Yang Yang | 2022 | Journal of Energy Chemistry2022，31，7： | 0 |
| 11 | Catalytically active atomically thin cuprate with periodic Cu single sites显示文摘Rational design and synthesis of catalytically active two-dimensional(2D)materials with an abundance of atomically precise active sites in their basal planes remains a great challenge.Here,we report a ligand exchange strategy to exfoliate bulk[Cu_(4)(OH)_(6)][O_(3)S(CH_(2))_4SO_(3)]cuprate crystals into atomically thin 2D cuprate layers([Cu_(2)(OH)_(3)]^(+)).The basal plane of 2D cuprate layers contains periodic arrays of accessible unsaturated Cu(Ⅱ)single sites(2D-CuSSs),which are found to promote efficient oxidative Chan-Lam coupling.Our me chanistic studies reveal that the reactions proceed via coordinatively unsaturated CuO_4(Ⅰ)single sites with the formation of Cu(Ⅰ)species in the rate-limiting step,as corroborated by both operando experimental and theoretical studies.The robust stability of 2D-CuSSs in both batch and continuous flow reactions,coupled with their recyclability and good performance in complex molecule derivatization,render 2D-CuSSs attractive catalyst candidates for broad utility in fine chemical synthesis. | Huimin Yang Shibo Xi Na Guo Mu Wang Lingmei Liu Pin Lyu Xiaolong Yu Jing Li Haomin Xu Xiao Hai Zejun Li Xinzhe Li Tao Sun Xiaoxu Zhao Yu Han Wei Yu Jie Wu Chun Zhang Honghan Fei Ming Joo Koh Jiong Lu | 2023 | National Science Review2023，10，1： | 0 |
| 12 | Involvement of triadimefon induced early ABA-dependent H2O2 accumulation in soybean against water stress显示文摘The present study showed that pretreatment of triadimefon(TDM),a triazole compound,could improve tolerance of soybean seedlings to subsequent water stress.TDM pretreatment resulted in early and late rise in superoxide dismutase(SOD)and catalase(CAT)activities,and upregulation of ascorbate(As A)content in non-stressed and water-stressed seedlings,leading to late increase in net photosynthetic rate(Pn),late decrease in hydrogen peroxide(H2O2)and electrolyte leakage in stressed ones.These TDM-induced changes were blocked by application of abscisic acid(ABA)biosynthesis inhibitor tungstate,which inhibited early rise of ABA and H2O2contents in non-stressed and stressed seedlings.However,ABA pretreatment overcomed the effects of this inhibitor.Application of NADPH oxidase inhibitor diphenyleneiodonium(DPI),polyamine oxidase(PAO)inhibitor 2-hydroxyethylhydrazine(2-HEH)and H2O2scavenger dimethylthiourea(DMTU)prevented early TDM-induced rise of H2O2content.DPI,2-HEH and DMTU also decreased SOD,CAT and As A levels,but did not affect ABA content during early and late phases in both seedlings pretreated with TDM.In addition,these chemicals decreased Pn,and increased H2O2content and electrolyte leakage during late phase in TDM-pretreated stressed seedlings.Overall,these results indicated that TDM pretreatment alleviated adverse effects of water stress on soybean seedlings,which was at least in part,due to increase of antioxidant capacity and decrease of oxidative damage induced by early ABA-dependent H2O2generation. | Zejun Xu Fei Tong Yujun Qi Donglei Sun Nengfei Bian Xing Wang | 2020 | Oil Crop Science2020，5，1： | 0 |
| 13 | TATA-box binding protein-associated factor 2 regulates grain size in rice显示文摘Grain size,characterized by a combination of grain length,width,and thickness,is one of the major determinants of yield in rice.The present study identified TATA-box binding protein-associated factor 2(TAF2)as an essential component regulating transcription and determining grain size in rice.Map-based cloning showed that a G/T substitution in TAF2 resulted in a naturally occurring mutant called reduced grain size and plant height 1(rgh1).The mutants,with weak edited rgh1 alleles,exhibited a small grain phenotype with reduced grain length and width,while the severe knockout mutant(rgh1-2s)was dwarf and completely sterile.Allelic test performed between rgh1 and several edited alleles confirmed that the mutation in TAF2 caused the rgh1 phenotype.GUS staining showed that TAF2 was mainly expressed in the vascular bundles of roots,stems,leaves,and grains.The cytological analysis revealed that reduced cell division in the glumes resulted in the small grain phenotype of rgh1.Further RNA-sequencing detected altered expression of genes involved in the basic biological processes in rgh1 mutant.These findings provide novel insights into the TAF2-mediated genetic mechanism regulating grain size in rice. | Ling Jiang Ning Jiang Zejun Hu Xuejun Sun Xian Xiang Yahui Liu Mingwei Wu Chunming Liu Xiaojin Luo | 2023 | The Crop Journal2023，11，2： | 0 |
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