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| 1 | Geological Characteristics and Ore-forming Time of the Dexing Porphyry Copper Ore Mine in Jiangxi Province显示文摘The Dexing porphyry copper ore mine is located in the Qin-Hang metallogenic belt between the Yangtze block and the Cathaysia block. It is a giant porphyry copper mine in China, including 3 ore districts: Tongchang, Fujiawu and Zhushahong. Our analyses of Re in molybdenite indicate that the ore-forming material of the copper ore deposits in Dexing should be mainly mantle-derived. Our study fills in a gap in the study of formation time of the Dexing copper mine, and further proves that the copper ore deposits in the three ore districts should be formed simultaneously, about 170 Ma, belonging to the early Yanshan period, and that the formation time of the copper ore deposits should be consistent with the formation time of granodiorite porphyry in which the copper ore deposits are hosted. Promising areas for seeking porphyry copper ore deposits is predicated to be the west or southwest of Dexing. | GUO Shuo ZHAO Yuany QU Huanchun WU Dexin XU Hong LI Chao LIU Yan ZHU Xiaoyun WANG Zengke | 2012 | Acta Geologica Sinica(English Edition)2012，86，3： | 28 |
| 2 | Seasonal patterns and environmental control of ecosystem respiration in subtropical and temperate forests in China显示文摘Continuous measurement of carbon dioxide exchange using the eddy covariance (EC) technique was made at two ChinaFLUX forest sites including the young subtropical Pinus plantation (Qianyanzhou) and old temperate broad-leaved Korean pine mixed forest (Changbai Mountains) as part of the ChinaFLUX network. Seasonal patterns and environmental control of ecosystem respiration in the subtropical and temperate forests were evaluated by the often-used multiplicative model and Q10 model as a function of temperature and soil water content. The resuits suggested that ( i ) temperature was found to be a dominant factor in the ecosystem respiration, and most of the temporal variability of ecosystem respiration was explained by temperature. However, in the drought-stressed ecosystem, soil water content controlled the temporal variability of ecosystem respiration other than temperature effects, and soil water content became a dominat factor when severe drought affected the ecosystem respiration; (ii) the regression models analysis revealed that in the drier soil, ecosystem respiration was more sensitive to soil moisture than was expressed by the often-used multiplicative model. It was possible to accurately estimate the seasonal variation of ecosystem respiration based on the Q10 model; and (iii)annual ecosystem respiration derived from the often-used multiplicative model was 1209 g C m-2and 1303 g C m-2, and was consistently a little higher than the Q10 model estimates of 1197 g C m-2 and 1268 g C m-2 for Qianyanzhou and Changbai Mountains, respectively. | YU Guirui, WEN Xuefa, LI Qingkang, ZHANG Leiming, REN Chuanyou, LIU Yunfen & GUAN Dexin Chinese Ecosystem Research Network Synthesis Research Center, Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing 100101, China Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China Graduate School of the Chinese Academy of Sciences, Beijing 100039, China | 2005 | Science China Earth Sciences2005，48，z1： | 13 |
| 3 | Single-shot BOTDA based on an optical chirp chain probe wave for distributed ultrafast measurement显示文摘Brillouin optical time-domain analysis(BOTDA)requires frequency mapping of the Brillouin spectrum to obtain environmental information(e.g.,temperature or strain)over the length of the sensing fiber,with the finite frequencysweeping time-limiting applications to only static or slowly varying strain or temperature environments.To solve this problem,we propose the use of an optical chirp chain probe wave to remove the requirement of frequency sweeping for the Brillouin spectrum,which enables distributed ultrafast strain measurement with a single pump pulse.The optical chirp chain is generated using a frequency-agile technique via a fast-frequency-changing microwave,which covers a larger frequency range around the Stokes frequency relative to the pump wave,so that a distributed Brillouin gain spectrum along the fiber is realized.Dynamic strain measurements for periodic mechanical vibration,mechanical shock,and a switch event are demonstrated at sampling rates of 25 kHz,2.5 MHz and 6.25 MHz,respectively.To the best of our knowledge,this is the first demonstration of distributed Brillouin strain sensing with a wide-dynamic range at a sampling rate of up to the MHz level. | Dengwang Zhou Yongkang Dong Benzhang Wang Chao Pang Dexin Ba Hongying Zhang Zhiwei Lu Hui Li Xiaoyi Bao | 2018 | Light(Science & Applications)2018，7，1： | 11 |
| 4 | Geochronology, Geochemistry and Sr-Nd-Pb-Hf Isotopes of No. I Complex from the Shitoukengde Ni–Cu Sulfide Deposit in the Eastern Kunlun Orogen, Western China: Implications for the Magmatic Source, Geodynamic Setting and Genesis显示文摘The Shitoukengde Ni-Cu deposit, located in the Eastern Kunlun Orogen, comprises three mafic–ultramafic complexes, with the No. I complex hosting six Ni-Cu orebodies found recently. The deposit is hosted in the small ultramafic bodies intruding Proterozoic metamorphic rocks. Complexes at Shitoukengde contain all kinds of mafic-ultramafic rocks, and olivine websterite and pyroxene peridotite are the most important Ni-Cu-hosted rocks. Zircon U-Pb dating suggests that the Shitoukengde Ni-Cu deposit formed in late Silurian(426–422 Ma), and their zircons have εHf(t) values of-9.4 to 5.9 with the older T_(DM1) ages(0.80–1.42 Ga). Mafic-ultramafic rocks from the No. I complex show the similar rare earth and trace element patterns, which are enriched in light rare earth elements and large ion lithophile elements(e.g., K, Rb, Th) and depleted in heavy rare earth elements and high field strength elements(e.g., Ta, Nb, Zr, Ti). Sulfides from the deposit have the slightly higher δ^(34)S values of 1.9–4.3‰ than the mantle(0 ± 2‰). The major and trace element characteristics, and Sr-Nd-Pb and Hf, S isotopes indicate that their parental magmas originated from a metasomatised, asthenospheric mantle source which had previously been modified by subduction-related fluids, and experienced significant crustal contamination both in the magma chamber and during ascent triggering S oversaturation by addition of S and Si, that resulted in the deposition and enrichment of sulfides. Combined with the tectonic evolution, we suggest that the Shitoukengde Ni-Cu deposit formed in the post-collisional, extensional regime related to the subducted oceanic slab break-off after the Wanbaogou oceanic basalt plateau collaged northward to the Qaidam Block in late Silurian. | LI Liang SUN Fengyue LI Bile LI Shijin CHEN Guangjun WANG Wei YAN Jiaming ZHAO Tuofei DONG Jun ZHANG Dexin | 2018 | Acta Geologica Sinica(English Edition)2018，92，1： | 10 |
| 5 | Correlation of the temporal and spatial expression patterns of HQT with the biosynthesis and accumulation of chlorogenic acid in Lonicera japonica flowers显示文摘Hydroxycinnamoyl-CoA quinate transferase(HQT)is one of the key enzymes in the biosynthesis of chlorogenic acid(CGA)in the flowers of Lonicera japonica.However,the spatiotemporal expression patterns of HQT and its relationship to the dynamics of CGA biosynthesis,transport,and storage remain largely unknown.In this study,we collected L.japonica flower samples at different growth stages(S1–S6)and examined the spatiotemporal expression pattern of HQT and the dynamic accumulation patterns of CGA using a combination of molecular and cytological techniques.Our results suggest that the spatiotemporal expression pattern of HQT is directly correlated with dynamic changes in CGA accumulation and distribution in L.japonica flowers.We further show that CGA is synthesized primarily in the cytoplasm and chloroplasts.CGA synthesized in the cytoplasm first accumulates in specialized vesicles and is then transferred to large central vacuoles for storage by fusion of CGA-containing vesicles with vacuoles.Furthermore,CGA synthesized in the chloroplasts appears to be transferred into the vacuoles for storage by direct membrane fusion between the tonoplast and the disrupted chloroplast membranes.Collectively,our results suggest that CGA is synthesized in chloroplasts and cytoplasm and finally transferred to the vacuole for long-term storage. | Yanqun Li Dexin Kong Mei Bai Hanjun He Haiyang Wang Hong Wu | 2019 | Horticulture Research2019，6，1： | 7 |
| 6 | Study on the Rainfall and Aftershock Threshold for Debris Flow of Post-earthquake显示文摘Due to the special condition of provenance and disaster environment after '5·12' Earthquake, the probability and conditions of the occurrence of gully debris flow change greatly after the event, which make it difficult to prevent disaster effectively. In this study the hydrological model of ground water table in loose sediment is established. According to infinite slope theory, the safety factor of deposits is defined as the ratio of resistance force to driving force. The starting condition of post-earthquake gully debris flow is clearly studied by analyzing the effects of rainfall intensity, seismic strength, slope gradient and mechanical properties on the balance of accumulation body. Then the formulas of rainfall and aftershock threshold for starting of gully debris flow are proposed, and an example is given to illustrate the effect of rainfall, aftershocks and their coupling action on a debris flow. The result shows the critical rainfall intensity decreases as the lateral seismic acceleration and channel gradient increases, while the critical intensity linearly increases as the friction angle increases. | HE Siming LI Dexin WU Yong LUO Yu | 2011 | Journal of Mountain Science2011，8，5： | 7 |
| 7 | Validity and reliability of Chinese version of Fatigue Impact Scale in cerebral infarction patients显示文摘BACKGROUND: Post-stroke fatigue has received much attention recently. Three evaluation scales are available,including the Fatigue Impact Scale (FIS). Yet none of these has been proven to be of use in the evaluation of psychological quality after post-stroke fatigue. OBJECTIVE: To introduce FIS into China,and to evaluate the validity and reliability of the Chinese Version in patients with cerebral infarction. DESIGN: Scale evaluation. SETTING: Department of Rehabilitation,Beijing Friendship Hospital,Capital Medical University. PARTICIPANTS: A total of 330 patients with cerebral infarction were selected from the Department of Neurology,Beijing Friendship Hospital,Capital Medical University from June 2005 to December 2006. All patients met diagnostic standards from the Fourth National Cerebrovascular Disease Academic Meeting of China Medical Association and were diagnosed with CT or MRI examination. Patients who were not willing to accept scale evaluation were excluded. METHODS: The English version of FIS was translated into Chinese and subsequently translated back to English again. FIS was given to the discharged patients,and they returned them upon completion. In-patients completed and immediately returned the FIS. Validity and reliability of the retrieved scales were ascertained with descriptive and inference analysis. MAIN OUTCOME MEASURES: Total scores and subscale scores of FIS. RESULTS: Out of 330 questionnaires,214 valid questionnaires were deemed valid in total. Six components were extracted in factor analysis,and the total cumulative contribution was 73.919%,which suggested the questionnaire was valid. After correlation analysis,6 components were divided into 3 subscales,including cognitive,physical,and social,which was the same as the English version. The Cronbach α value for the three subscales was 0.937 7,0.918 8,and 0.940 6,respectively (> 0.7). CONCLUSION: The reliability and validity of the Chinese version of FIS met with satisfaction and ap-peared to be adaptable to cerebral infarction patients in China. | Chunwei Wu Zhandong Liu Yongbo Zhang Jimei Li Dexin Wang | 2008 | Neural Regeneration Research2008，3，2： | 6 |
| 8 | Hemorrhagic Fever with Renal Syndrome — Liaoning Province, China, 1999−2018显示文摘Summary What is already known on this topic?Hemorrhagic fever with renal syndrome(HFRS)is endemic in Liaoning Province.Both Seoul and Hantaan virus are circulating in rodents,and epidemic outbreaks and sporadic cases have been recorded every year since the disease was recognized. | Cui Shang Yingwei Sun Qiangling Yin Xiaoxia Huang Xuesheng Liu Quanfu Zhang Lingling Mao Chuan Li Aqian Li Qin Wang Lina Sun Mifang Liang Shiwen Wang Dexin Li Jiandong Li | 2020 | China CDC weekly2020，2，20： | 6 |
| 9 | Differential gene expression in proximal and distal nerve segments of rats with sciatic nerve injury during Wallerian degeneration显示文摘Wallerian degeneration is a subject of major interest in neuroscience. A large number of genes are differentially regulated during the distinct stages of Wallerian degeneration: transcription factor activation, immune response, myelin cell differentiation and dedifferentiation. Although gene expression responses in the distal segment of the sciatic nerve after peripheral nerve injury are known, differences in gene expression between the proximal and distal segments remain unclear. In the present study in rats, we used microarrays to analyze changes in gene expression, biological processes and signaling pathways in the proximal and distal segments of sciatic nerves undergoing Wallerian degeneration. More than 6,000 genes were differentially expressed and 20 types of expression tendencies were identified, mainly between proximal and distal segments at 7–14 days after injury. The differentially expressed genes were those involved in cell differentiation, cytokinesis, neuron differentiation, nerve development and axon regeneration. Furthermore, 11 biological processes were represented, related to responses to stimuli, cell apoptosis, inflammatory response, immune response, signal transduction, protein kinase activity, and cell proliferation. Using real-time quantitative PCR, western blot analysis and immunohistochemistry, microarray data were verified for four genes: aquaporin-4, interleukin 1 receptor-like 1, matrix metalloproteinase-12 and periaxin. Our study identifies differential gene expression in the proximal and distal segments of a nerve during Wallerian degeneration, analyzes dynamic biological changes of these genes, and provides a useful platform for the detailed study of nerve injury and repair during Wallerian degeneration. | Nan Jiang Huaiqin Li Yi Sun Dexin Yin Qin Zhao Shusen Cui Dengbing Yao | 2014 | Neural Regeneration Research2014，9，12： | 5 |
| 10 | Anoxic degradation of nitrogenous heterocyclic compounds by activated sludge and their active sites显示文摘The potential for degradation of five nitrogenous heterocyclic compounds(NHCs), i.e.,imidazole, pyridine, indole, quinoline, and carbazole, was investigated under anoxic conditions with acclimated activated sludge. Results showed that NHCs with initial concentration of 50 mg/L could be completely degraded within 60 hr. The degradation of five NHCs was dependent upon the chemical structures with the following sequence:imidazole > pyridine > indole > quinoline > carbazole in terms of their degradation rates.Quantitative structure–biodegradability relationship studies of the five NHCs showed that the anoxic degradation rates were correlated well with highest occupied molecular orbital.Additionally, the active sites of NHCs identified by calculation were confirmed by analysis of intermediates using gas chromatography and mass spectrometry. | Peng Xu Hongjun Han Haifeng Zhuang Baolin Hou Shengyong Jia Dexin Wang Kun Li Qian Zhao | 2015 | Journal of Environmental Sciences2015，27，5： | 5 |
| 11 | High-performance optical chirp chain BOTDA by using a pattern recognition algorithm and the differential pulse-width pair technique显示文摘Optical chirp chain Brillouin optical time-domain analysis(OCC-BOTDA) has the capabilities of fast measurement, high Brillouin threshold, and freedom from the nonlocal effect;at the same time, however, it also has problems introduced by transient stimulated Brillouin scattering. The influence of the transient interaction is reflected as the broadened asymmetric Brillouin spectrum, the ghost peak, and the frequency shift of the main peak. This introduces difficulty in computing the fiber Brillouin frequency shift with good measurement accuracy. Besides, the OCC modulation causes additional noise due to the uneven amplitude response for different frequency components. In this work, we propose a high-performance OCC-BOTDA using the principal component analysis(PCA) based pattern recognition algorithm and differential pulse-width pair(DPP) technique.After building the Brillouin spectrum database(i.e., all patterns), the fiber intrinsic Brillouin frequency shift can be recognized by the PCA algorithm from a nonstandard Brillouin spectrum profile, resulting in good measurement accuracy. Meanwhile, the DPP technique, subtracting between two Brillouin signals generated by two wide-width pump pulses, is utilized to reduce the OCC modulation noise and avoid the pulse self-phase modulation effect in long-range BOTDA sensing. In the experiment, a temperature measurement with 1.3 MHz measurement precision, 4 m spatial resolution, and 5 s measurement time is achieved over a 100 km single-mode fiber. | BENZHANG WANG BAOHUA FAN DENGWANG ZHOU CHAO PANG YUE LI DEXIN BA YONGKANG DONG | 2019 | Photonics Research2019，7，6： | 5 |
| 12 | Modelling of tropospheric delays in geosynchronous synthetic aperture radar显示文摘As a direct consequence of the orbital height, the integration time in geosynchronous synthetic aperture radar(GEO SAR) with metric or decimetric azimuth resolutions is in the order of several hundreds or even thousands of seconds. With such long integration time, the compensation of residual tropospheric propagation terms poses one of the fundamental challenges associated with GEO SAR missions. In order to better characterise the impact of the propagation errors on GEO SAR imaging, we put forward a model for the simulation of the tropospheric delay appropriate for the accurate simulation of GEO SAR surveys. The suggested model, with a deterministic background component and a random turbulent one, incorporates some of the most recent meteorological data for the characterization of the troposphere. To illustrate the relevance of the derivation, the suggested model is used for performance estimation and raw data simulation of GEO SAR raw data. Substantial conclusion on the system impulse response and the associated calibration requirements is also drawn from the analysis. | Dexin LI Marc RODRIGUEZ-CASSOLA Pau PRATS-IRAOLA Zhen DONG Manqing WU Alberto MOREIRA | 2017 | Science China(Information Sciences)2017，60，6： | 4 |
| 13 | Nasal delivery of broadly neutralizing antibodies protects mice from lethal challenge with SARS-CoV-2 delta and omicron variants显示文摘Multiple new variants of severe acute respiratory syndrome coronavirus 2(SARS-Co V-2)have constantly emerged,as the delta and omicron variants,which have developed resistance to currently gained neutralizing antibodies.This highlights a critical need to discover new therapeutic agents to overcome the variants mutations.Despite the availability of vaccines against coronavirus disease 2019(COVID-19),the use of broadly neutralizing antibodies has been considered as an alternative way for the prevention or treatment of SARS-Co V-2 variants infection.Here,we show that the nasal delivery of two previously characterized broadly neutralizing antibodies(F61 and H121)protected K18-h ACE2 mice against lethal challenge with SARS-Co V-2 variants.The broadly protective efficacy of the F61 or F61/F121 cocktail antibodies was evaluated by lethal challenge with the wild strain(WIV04)and multiple variants,including beta(B.1.351),delta(B.1.617.2),and omicron(B.1.1.529)at 200or 1000 TCID_(50),and the minimum antibody administration doses(5-1.25 mg/kg body weight)were also evaluated with delta and omicron challenge.Fully prophylactic protections were found in all challenged groups with both F61 and F61/H121 combination at the administration dose of 20 mg/kg body weight,and corresponding mice lung viral RNA showed negative,with almost all alveolar septa and cavities remaining normal.Furthermore,low-dose antibody treatment induced significant prophylactic protection against lethal challenge with delta and omicron variants,whereas the F61/H121 combination showed excellent results against omicron infection.Our findings indicated the potential use of broadly neutralizing monoclonal antibodies as prophylactic and therapeutic agent for protection of current emerged SARS-Co V-2 variants infection. | Jia Lu Qiangling Yin Rongjuan Pei Qiu Zhang Yuanyuan Qu Yongbing Pan Lina Sun Ding Gao Cuiqin Liang Jingwen Yang Wei Wu Jiandong Li Zongqiang Cui Zejun Wang Xinguo Li Dexin Li Shiwen Wang Kai Duan Wuxiang Guan Mifang Liang Xiaoming Yang | 2022 | Virologica Sinica2022，37，2： | 4 |
| 14 | Triassic Granitic Magmatism at the Northern Margin of the North China Craton: Implications of Geochronology and Geochemistry for the Tectonic Evolution of the Central Asian Orogenic Belt显示文摘The early Mesozoic marked an important transition from collisional orogeny to post-orogenic extension at the northern margin of the North China Craton(NCC). In this study, we undertook zircon U-Pb dating and whole-rock majorand trace-element geochemical analyses of early Mesozoic granitic rocks in the Chifeng area to establish their geochronological framework, petrogenesis, and implications for the tectonic evolution of the eastern Central Asia Orogenic Belt(CAOB). Zircon U-Pb dating results show that these rocks were emplaced in three stages during the Triassic:(1) syenogranites during 250-248 Ma,(2) granodiorites during 244-243 Ma, and(3) monzogranites and granodiorites during 232-230 Ma. These Triassic granitoids belong to the high-K calc-alkaline series and are evolved I-type granites. They have high SiO2 and low Mg O contents with enrichments in light rare-earth elements, Zr, Hf, Rb, Th, and U, and depletions in Ba, Nb, Ta, Sr, and Eu. These geochemical data indicate that the granitoids were derived from partial melting of a lower-crustal source under relatively low-pressure conditions and subsequently underwent extensive fractional crystallization. Considering both the geochemical data and regional geological information, we propose that the 250-248 Ma syenogranites were emplaced in an extensional environment linked to slab break-off after closure of the Paleo-Asian Ocean(PAO) along the Solonker-Xra Moron-Changchun suture zone. The 244-243 Ma granodiorites were formed in a compressional orogenic setting during collision between the Erguna-Xing’an-Songliao composite block and the NCC. The 232-230 Ma granodiorites and monzogranites were emplaced during the transition from compressional orogeny to post-orogenic extension. Overall, the early Mesozoic tectonic evolution of the Chifeng area can be divided into three main stages:(1) closure of the Paleo-Asian Ocean and extension related to slab break-off during the Early Triassic;(2) continuous collisional compression during the Middle Triassic after closure of the PAO;and(3) post-orogenic extension during the Late Triassic, most probably due to lithospheric delamination after amalgamation of the Erguna-Xing’an-Songliao composite block and the NCC. | CHEN Jingsheng TIAN Dexin YANG Hao LI Weiwei LIU Miao LI Bin YANG Fan LI Wei WU Zhen | 2019 | Acta Geologica Sinica(English Edition)2019，93，5： | 4 |
| 15 | Effects of temperature and light on seed germination of ephemeral plants in the Gurbantunggut Desert, China: implications for vegetation restoration显示文摘Seed germination is a key transitional stage in plant life cycle and is strongly regulated by temperature and light.Therefore, research on the effects of temperature and light on seed germination is extremely meaningful for vegetation restoration, especially in desert ecosystems.Seeds of 28 ephemeral plants collected from the Gurbantunggut Desert of China were incubated at different temperatures(5℃/1℃, 15℃/5℃, 20℃/5℃, 25℃/10℃ and 30℃/15℃) in 12-h light/12-h darkness or continuous darkness regimes, and the responses of seed germination to temperature and light and the germination speed were studied in 2016.Results showed that seed germination percentage of the 28 ephemeral plants significantly differed to temperature and light.We classified the studied plants as the following groups based on their responses to temperature: 1 low temperature responsed plants, 12 moderate temperature responsed plants, 7 high temperature responsed plants, 4 non-responsed plants and 5 plants of no germination.It should be noted that Corispermum lehmannianum Bunge is sensitive to both moderate and high temperatures.There were 4 groups of plant in response to light, i.e., 7 light responsed plants, 10 dark responsed plants, 6 light non-responsed plants and 5 plants of no germination.Based on seed germination speed of the 28 ephemeral plants, we divided them into 4 patterns of germination, i.e., very rapid, moderately rapid, moderate and slow.Combining variations of temperature, precipitation and sand dune types in the study area, we suggested that very rapid and moderately rapid germinated plants could be used to moving sand dunes in early spring during vegetation restoration, moderate germinated plants could be used to semi-fixed sand dunes in late autumn, and slow germinated plants could be used to sand plain in summer.Thus, seedling establishment and vegetation restoration would be improved by considering seed germination characteristics of these ephemeral plants in the Gurbantunggut Desert, China. | CHEN Yanfeng CAO Qiumei LI Dexin LIU Huiliang ZHANG Daoyuan | 2019 | Journal of Arid Land2019，11，6： | 4 |
| 16 | Molecular evolution and genetic diversity analysis of SFTS virus based on next-generation sequencing显示文摘SFTS virus(SFTSV)is a novel bunyavirus,which was discovered as the etiological agent of severe fever with thrombocytopenia syndrome(SFTS)in China in 2009,and was now prevalent in at least 25 provinces in China.SFTS was subsequently identified in South Korea and Japan in 2012.To explore themolecular evolution and genetic characteristics of this newly identified pathogen,we reported 72 whole genome sequences of SFTSV,and built a dataset of SFTSV genome sequences containing 292 L-segment,302 M-segment and 502 S-segment.We clearly divided SFTSV into six genotypes,Genotype A-F.It was found that genotype F was the dominant epidemic genotype of Japan,South Korea,and Zhejiang province of China.The coalescent analysis supported that SFTSV originated in the early 18th century from Zhejiang province,and Genotype F was the most primitive one.Henan,Hubei,and Anhui provinces which are located in Dabie Mountain area weremainly epidemic of Genotype A,which emerged relatively late but distributed widely.A total of 37 recombination events were identified,making SFTSV with a high recombination frequency(L segment 5.1%,Msegment 3.6%,S segment 0.8%)among negative-strand segmented RNA viruses.It was identified that 19 reassortant strains belonged to 12 reassortment forms of SFTSV genome containing 6 newly identified forms.The reassortment virus and recombination in tick were both found for the first time.We also found many of genotype-specific mutation sites,7 of which could be considered as potential molecular marker for genotype classification.This study promoted a more comprehensive understanding of the phylogeny and origin,and the genetic diversity of SFTSV,and it could help the studies of other newly discovered tick-borne bunyavirus as reference data and research ideas. | Aqian Li Lin Liu Wei Wu Yang Liu Xiaoxia Huang Chuan Li Di Liu Jiandong Li Shiwen Wang Dexin Li Mifang Liang | 2021 | Biosafety and Health2021，3，2： | 4 |
| 17 | SNX11 Identified as an Essential Host Factor for SFTS Virus Infection by CRISPR Knockout Screening显示文摘Severe fever with thrombocytopenia syndrome virus(SFTSV)is a highly pathogenic tick-borne bunyavirus that causes lethal infectious disease and severe fever with thrombocytopenia syndrome(SFTS)in humans.The molecular mechanisms and host cellular factors required for SFTSV infection remain uncharacterized.Using a genome-wide CRISPR-based screening strategy,we identified a host cellular protein,sorting nexin 11(SNX11)which is involved in the intracellular endosomal trafficking pathway,as an essential cell factor for SFTSV infection.An SNX11-KO HeLa cell line was established,and SFTSV replication was significantly reduced.The glycoproteins of SFTSV were detected and remained in later endosomal compartments but were not detectable in the endoplasmic reticulum(ER)or Golgi apparatus.pH values in the endosomal compartments of the SNX11-KO cells increased compared with the pH of normal HeLa cells,and lysosomal-associated membrane protein 1(LAMP1)expression was significantly elevated in the SNX11-KO cells.Overall,these results indicated that penetration of SFTSV from the endolysosomes into the cytoplasm of host cells was blocked in the cells lacking SNX11.Our study for the first time provides insight into the important role of the SNX11 as an essential host factor in the intracellular trafficking and penetrating process of SFTSV infection via potential regulation of viral protein sorting,membrane fusion,and other endocytic machinery. | Tiezhu Liu Jiajia Li Yang Liu Yuanyuan Qu Aqian Li Chuan Li Quanfu Zhang Wei Wu Jiandong Li Yan Liu Dexin Li Shiwen Wang Mifang Liang | 2019 | Virologica Sinica2019，34，5： | 3 |
| 18 | Antibody Cocktail Exhibits Broad Neutralization Activity Against SARS-CoV-2 and SARS-CoV-2 Variants显示文摘Severe acute respiratory syndrome coronavirus 2(SARS-Co V-2)has precipitated multiple variants resistant to therapeutic antibodies.In this study,12 high-affinity antibodies were generated from convalescent donors in early outbreaks using immune antibody phage display libraries.Of them,two RBD-binding antibodies(F61 and H121)showed high-affinity neutralization against SARS-Co V-2,whereas three S2-target antibodies failed to neutralize SARS-Co V-2.Following structure analysis,F61 identified a linear epitope located in residues G446–S494,which overlapped with angiotensinconverting enzyme 2(ACE2)binding sites,while H121 recognized a conformational epitope located on the side face of RBD,outside from ACE2 binding domain.Hence the cocktail of the two antibodies achieved better performance of neutralization to SARS-Co V-2.Importantly,these two antibodies also showed efficient neutralizing activities to the variants including B.1.1.7 and B.1.351,and reacted with mutations of N501 Y,E484 K,and L452 R,indicated that it may also neutralize the recent India endemic strain B.1.617.The unchanged binding activity of F61 and H121 to RBD with multiple mutations revealed a broad neutralizing activity against variants,which mitigated the risk of viral escape.Our findings revealed the therapeutic basis of cocktail antibodies against constantly emerging SARS-Co V-2 variants and provided promising candidate antibodies to clinical treatment of COVID-19 patients infected with broad SARS-Co V-2 variants. | Yuanyuan Qu Xueyan Zhang Meiyu Wang Lina Sun Yongzhong Jiang Cheng Li Wei Wu Zhen Chen Qiangling Yin Xiaolin Jiang Yang Liu Chuan Li Jiandong Li Tianlei Ying Dexin Li Faxian Zhan Youchun Wang Wuxiang Guan Shiwen Wang Mifang Liang | 2021 | Virologica Sinica2021，36，5： | 3 |
| 19 | Hundred-Joule-level,nanosecond-pulse Nd:glass laser system with high spatiotemporal beam quality显示文摘A 100-J-level Nd:glass laser system in nanosecond-scale pulse width has been constructed to perform as a standard source of high-fluence-laser science experiments. The laser system, operating with typical pulse durations of 3–5 ns and beam diameter 60 mm, employs a sequence of successive rod amplifiers to achieve 100-J-level energy at 1053 nm at3 ns. The frequency conversion can provide energy of 50-J level at 351 nm. In addition to the high stability of the energy output, the most valuable of the laser system is the high spatiotemporal beam quality of the output, which contains the uniform square pulse waveform, the uniform flat-top spatial fluence distribution and the uniform flat-top wavefront. | Sensen Li Yulei Wang Zhiwei Lu Lei Ding Yi Chen Pengyuan Du Dexin Ba Zhenxing Zheng Xin Wang Hang Yuan Chengyu Zhu Weiming He Dianyang Lin Yongkang Dong Dengwang Zhou Zhenxu Bai Zhaohong Liu Can Cui | 2016 | High Power Laser Science and Engineering2016，4，1： | 3 |
| 20 | Serological Investigation of Laboratory-Confirmed and Suspected Ebola Virus Disease Patients During the Late Phase of the Ebola Outbreak in Sierra Leone显示文摘This study aimed to investigate the serological characteristics of Ebola virus(EBOV) infection during the late phase of the Ebola outbreak in Sierra Leone. In total, 877 blood samples from 694 suspected Ebola virus disease(EVD) cases assessed from March to December 2015, were analyzed via real-time reverse transcription polymerase chain reaction(RT-PCR) for viral RNA and enzyme-linked immunosorbent assay(ELISA) and Luminex to detect antibodies against EBOV. Viral load and EBOV-specific IgM/IgG titers displayed a declining trend during March to December 2015. Viral RNA load decreased rapidly at earlier stages after disease onset, while EBOV-specific IgM and IgG still persisted in 58.1%(18/31) and 93.5%(29/31) of the confirmed EVD patients and in 3.8%(25/663) and 17.8%(118/663) of the RNA-negative suspected patients in the later phase, respectively. Dynamic analysis of longitudinally collected samples from eight EVD patients revealed typically reversed trends of declining viral load and increasing IgM and/or IgG titers in response to the EBOV infection.The present results indicate that certain populations of Sierra Leone developed immunity to an EBOV infection in the late phase of the outbreak, providing novel insights into the risk assessment of EBOV infections among human populations. | Yang Liu YuLan Sun Wei Wu AQian Li XianDa Yang Shuo Zhang Chuan Li QiuDong Su ShaoJian Cai DaPeng Sun HaiYang Hu Zhe Zhang XiuXu Yang Idrissa Kamara Sheku Koroma Gerald Bangura Alie Tia Abdul Kamara Matt Lebby Brima Kargbo Jiandong Li Shiwen Wang XiaoPing Dong YueLong Shu WenBo Xu George F. Gao GuiZhen Wu DeXin Li William J. Liu MiFang Liang | 2018 | Virologica Sinica2018，33，4： | 2 |
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