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| 1 | 中国水稻遗传育种历程与展望显示文摘我国的水稻育种经历了矮化育种、杂种优势利用和绿色超级稻培育3次飞跃,其间伴随矮化育种(第一次绿色革命)、三系杂交稻培育、二系杂交稻培育、亚种间杂种优势利用、理想株型育种和绿色超级稻培育等6个重要历程。育种目标从唯产量是举到高抗、优质和高产并重,育种理念从高产优质逐步提升为'少投入,多产出,保护环境'。水稻功能基因组研究为第二次绿色革命准备了大量的有重要利用价值的基因,水稻育种正迈向设计育种的新时代。基因组选择技术和转基因技术将为培育'少打农药,少施化肥,节水抗旱,优质高产'绿色超级稻保驾护航。本文对我国水稻遗传育种的发展历程进行了概括,指出了各种育种方法和育种技术的优缺点,系统介绍了水稻细胞质雄性不育和光温敏雄性核不育以及籼粳杂种不育的分子机制的研究进展,综述了水稻株型、穗型、粒形和养分高效利用相关的重要功能基因,阐明了产量与开花期联动的关系,凸显了我国水稻基础研究在国际上的重要地位。特别指出,近年来,我国水稻生产方式发生了或正在发生巨大变革,育种理念也要与时俱进。未来,杂交育种技术要与现代育种技术紧密结合,选育水稻品种不仅要满足市场需求,而且更要具备绿色健康的特点,同时还要适应新耕作制度和新耕作方法。 | 吴比 胡伟 邢永忠 | 2018 | 遗传2018，40，10： | 66 |
| 2 | GS6, A Member of the GRAS Gene Family,Negatively Regulates Grain Size in Rice显示文摘Grain size is an important yield related trait in rice.Intensive arti cial selection for grain size during domestication is evidenced by the larger grains of most of today’s cultivars compared with their wild relatives.However,the molecular genetic control of rice grain size is still not well characterized.Here,we report the identi cation and cloning of Grain Size 6(GS6),which plays an important role in reducing grain size in rice.A premature stop at the t348 position in the coding sequence(CDS)of GS6 increased grain width and weight signi cantly.Alignment of the CDS regions of GS6 in 90 rice materials revealed three GS6alleles.Most japonica varieties(95%)harbor the Type I haplotype,and 62.9%of indica varieties harbor the Type II haplotype.Association analysis revealed that the Type I haplotype tends to increase the width and weight of grains more than either of the Type II or Type III haplotypes.Further investigation of genetic diversity and the evolutionary mechanisms of GS6showed that the GS6gene was strongly selected in japonica cultivars.In addition,a'ggc'repeat region identi ed in the region that encodes the GRAS domain of GS6 played an important historic role in the domestication of grain size in rice.Knowledge of the function of GS6might aid efforts to elucidate the molecular mechanisms that control grain development and evolution in rice plants,and could facilitate the genetic improvement of rice yield. | Lianjun Sun Xiaojiao Li Yongcai Fu Zuofeng Zhu Lubin Tan Fengxia Liu Xianyou Sun Xuewen Sun Chuanqing Sun | 2013 | Journal of Integrative Plant Biology2013，55，10： | 44 |
| 3 | A Novel QTL qTGW3 Encodes the GSK3/ SHAGGY-Like Kinase OsGSK5/OsSK41 that Interacts with OsARF4 to Negatively Regulate Grain Size and Weight in Rice显示文摘谷物尺寸和形状在米饭是谷物重量和产量的重要决定因素。这里,我们报导一个新主要量的特点在米饭的地点(QTL ) , qTGW3,那种控制谷物尺寸和重量。这个地点, qTGW3,编码 OsSK41 (也作为 OsGSK5 知道) , GLYCOGEN SYNTHASE KINASE 3/SHAGGY-like 家庭的一个成员。带 OsSK41 的 loss-of-function 等位基因的瑞斯 near-isogenic 线增加了谷物长度和重量。我们证明那 OsSK41 交往与并且 phosphorylates 植物生长素反应因素 4 (OsARF4 ) 。有 OsARF4 的 OsSK41 的合作表示在米饭原物增加 OsARF4 的累积。OsARF4 的功能的损失导致更大的米饭谷物。定序 RNA 分析建议 OsARF4 和 OsSK41 镇压下游的基因的一个普通集合的表示, ? 包括一些植物生长素应答的基因,在米饭谷物开发期间。在 qTGW3 的 OsSK41 的 loss-of-function 形式代表没被指向的基因编辑或 QTL 节节上升广泛地在 OsSK41 功能的米饭 breeding.?Suppression 利用了的稀罕等位基因提高米饭谷物尺寸和重量。因此,我们的学习在米饭谷物开发揭示 OsSK41 的重要角色 ?? 并且在另外的谷物庄稼在米饭并且也许为谷物产量的基因改进提供新候选人基因。 | Zejun Hu Sun-Jie Lu Mei-Jing Wang Haohua He Le Sun Hongru Wang Xue-Huan Liu Ling Jiang Jing-Liang sun Xiaoyun Xin Wei Kong Chengcai Chu Hong-Wei Xue Jinshui Yang Xiaojin Luo Jian-Xiang Liu | 2018 | Molecular Plant2018，11，5： | 47 |
| 4 | 基于CRISPR/Cas9技术的水稻千粒重基因tgw6突变体的创建显示文摘利用CRISPR/Cas9技术对调控水稻产量千粒重基因TGW6定点编辑,获得了一套有重要育种价值的tgw6突变体。设计了分别由U3、U6a和U6b启动子驱动、长20 bp的guide RNA(g RNA)靶点以靶向编辑TGW6基因的外显子,首先将这3个靶点一起组装到p YLCRISPR/Cas9-MT(I)载体上,然后利用农杆菌介导侵染水稻材料H447(R819/玉针香//R819的BC3F6);提取T0代转基因植株的基因组DNA并对编辑位点附近的DNA片段进行PCR检测及测序分析。结果表明,T0代材料中tgw6的突变频率高达90%,其中纯合缺失突变率约占51%。对T1代纯合缺失突变体的千粒重性状的调查分析结果表明,部分tgw6的缺失突变能显著提高千粒重(大于5%)。不同类型tgw6突变体的成功创建不仅丰富了tgw6的变异类型,为水稻的高产稳产奠定了重要的材料基础,还证实了CRISPR/Cas9技术在水稻基因工程育种中高效、易操作的特点。 | 王加峰 郑才敏 刘维 罗文龙 王慧 陈志强 郭涛 | 2016 | 作物学报2016，42，8： | 31 |
| 5 | Molecular dissection of complex agronomic traits of rice:a team effort by Chinese scientists in recent years显示文摘Rice is a staple food for more than half of the worldwide population and is also a model species for biological studies on monocotyledons. hrough a team efort, Chinese scientists have made rapid and important progresses in rice biology in recent years. Here, we briely review these advances, emphasizing on the regulatory mechanisms of the complex agronomic traits that afect rice yield and grain quality. Progresses in rice genome biology and genome evolution have also been summarized. | Jianru Zuo Jiayang Li | 2014 | National Science Review2014，1，2： | 26 |
| 6 | Validation and Characterization of Ghd7.1, a Major Quantitative Trait Locus with Pleiotropic Effects on Spikelets per Panicle, Plant Height, and Heading Date in Rice (Oryza sativa L.)显示文摘A quantitative trait locus(QTL)that affects heading date(HD)and the number of spikelets per panicle(SPP)was previously identifed in a small region on chromosome 7 in rice(Oryza sativa L.).In order to further characterize the QTL region,near isogenic lines(NILs)were quickly obtained by self‐crossing recombinant inbred line 189,which is heterozygous in the vicinity of the target region.The pleiotropic effects of QTL Ghd7.1 on plant height(PH),SPP,and HD,were validated using an NIL‐F2 population.Ghd7.1 explained 50.2%,45.3%,and 76.9% of phenotypic variation in PH,SPP,and HD,respectively.Ghd7.1 was precisely mapped to a 357‐kb region on the basis of analysis of the progeny of the NIL‐F2 population.Day‐length treatment confrmed that Ghd7.1 is sensitive to photoperiod,with long days delaying heading up to 12.5 d.Identifcation of panicle initiation and development for the pair of NILs showed that Ghd7.1 elongated the photoperiod‐sensitive phase more than 10 d,but did not change the basic vegetative phase and the reproductive growth phase.These fndings indicated that Ghd7.1 regulates SPP by controlling the rate of panicle differentiation rather than the duration of panicle development. | Touming Liu Haiyang Liu Huang Zhang Yongzhong Xing | 2013 | Journal of Integrative Plant Biology2013，55，10： | 22 |
| 7 | 水稻粒型基因克隆和调控机制研究进展显示文摘水稻粒型是影响其产量和品质的重要性状,阐明其遗传调控机理,有助于提高水稻单产和改良品质。水稻粒型性状主要包括粒长、粒宽、粒厚、长/宽比,属于数量性状,受胚、胚乳及母体植株等不同遗传体系的控制。随着水稻功能基因组学和重测序技术的快速发展,目前已经定位超过400个与水稻粒型相关的数量性状位点(QTL),并已克隆了60个水稻粒型基因,涉及植物激素、泛素-蛋白酶体通路、丝裂原活化蛋白激酶(MAPK)信号通路、G蛋白信号通路及表观修饰等多个调控通路。本文对水稻粒型基因克隆及其调控机制的研究进展进行了系统总结和梳理,并对这些基因在育种上的利用价值进行了评价。 | 刘喜 牟昌铃 周春雷 程治军 江玲 万建民 | 2018 | 中国水稻科学2018，32，1： | 22 |
| 8 | Identification and fine mapping of quantitative trait loci for seed vigor in germination and seedling establishment in rice显示文摘Seed vigor is an index of seed quality that is used to describe the rapid and uniform germination and the establishment of strong seedlings in any environmental conditions.Strong seed vigor in low-temperature germination conditions is particularly important in direct-sowing rice production systems. However, seed vigor has not been selected as an important breeding trait in traditional breeding programs due to its quantitative inherence. In this study, we identified and mapped eight quantitative trait loci(QTLs) for seed vigor by using a recombinant inbred population from a cross between rice(Oryza sativa L. ssp. indica) cultivars ZS97 and MH63.Conditional QTL analysis identified qSV-1, qSV-5b, qSV-6a, qSV-6b, and qSV-11 influenced seedling establishment and that qSV-5a, qSV-5c, and qSV-8 influenced only germination. Of these,qSV-1, qSV-5b, qSV-6a, qSV-6b, and qSV-8 were low-temperature-specific QTLs. Two major-effective QTLs, qSV-1, and qSV-5c were narrowed down to 1.13-Mbp and 400-kbp genomic regions, respectively. The results provide tightly linked DNA markers for the marker-assistant pyramiding of multiple positive alleles for increased seed vigor in both normal and low-temperature germination environments. | Lixia Xie Zhengwei Tan Yuan Zhou Rongbao Xu Laibao Feng Yongzhong Xing Xiaoquan Qi | 2014 | Journal of Integrative Plant Biology2014，56，8： | 17 |
| 9 | The GW2-WG1-OsbZIP47 pathway controls grain size and weight in rice显示文摘Regulation of seed size is a key strategy for improving crop yield and is also a basic biological question.However,the molecular mechanisms by which plants determine their seed size remain elusive.Here,we report that the GW2-WG1-OsbZIP47 regulatory module controls grain width and weight in rice.WG1,which encodes a glutaredoxin protein,promotes grain growth by increasing cell proliferation.Interestingly,WG1 interacts with the transcription factor OsbZIP47 and represses its transcriptional activity by associating with the transcriptional co-repressor ASP1,indicating that WG1 may act as an adaptor protein to recruit the transcriptional co-repressor.In contrary,OsbZIP47 restricts grain growth by decreasing cell proliferation.Further studies reveal that the E3 ubiquitin ligase GW2 ubiquitinates WG1 and targets it for degradation.Genetic analyses confirm that GW2,WG1,and OsbZIP47 function in a comm on pathway to control grain growth.Taken together,ourfindi ngs reveal a genetic and molecular framework for the control of grain size and weight by the GW2-WG1-OsbZIP47 regulatory module,providing new targets for improving seed size and weight in crops. | Jianqin Hao Dekai Wang Yingbao Wu Ke Huang Penggen Duan Na Li Ran Xu Dali Zeng Guojun Dong Baolan Zhang Limin Zhang Dirk Inze Qian Qian Yunhai Li | 2021 | Molecular Plant2021，14，8： | 14 |
| 10 | 水稻粒型基因克隆研究进展及育种应用展望显示文摘水稻的育种目标包括高产和优质等。粒型是指籽粒的三维结构,是决定水稻产量的重要因素之一,也是影响稻米品质的重要性状。目前已经克隆了超过60个与水稻粒型相关的基因,但应用于育种的粒型基因尚无完整统计。本文综述了已克隆的水稻粒型基因特别是数量性状基因座的研究进展,归纳总结了可应用于水稻高产及优质分子育种的重要粒型基因的功能标记,介绍了粒型基因的育种应用现状,并对前景作了展望,以期为水稻育种家提供可直接利用的分子育种信息。 | 康艺维 陈玉宇 张迎信 | 2020 | 中国水稻科学2020，34，6： | 13 |
| 11 | Mapping Heterotic Loci for Yield and Agronomic Traits Using Chromosome Segment Introgression Lines in Cotton显示文摘在现在的学习,一套染色体片断基因渗入排队(CSIL ) 使用 Gossypium hirsutum L。TM-1 作为接受者父母和 G。施主父母被用来为种间的混血儿探索杂种优势的基因基础的 barbadense Hai7124。个别地从与两个父母一起交叉的 CSIL 导出的 F1 人口的二个集合被设置调查 heterotic loci (HL ) 和替换效果 loci (SL ) 。58 HL 和 39 SL 的一个总数在 3 年里被识别。一稳定的 HL, hLP-A4-3,能在所有 3 年里被检测。三 HL, hBS-A8-1, hLP-D6-1,和 hSI-D7-11,能在 2 年里被检测。四 SL, sBS-D7-1, sLP-A8-1, sLP-D7-1,和 sLP-D12-1,能在 2 年里被检测。HL 和 SL 趋于在有靠近的位置的一些 HL 富有的染色体片断分布式。与在以前的研究检测的 QTL 相比, HL 与 QTL 显示出很少重叠,显示特点显型和杂种优势可能被 loci 的不同集合控制。基因效果的所有三种形式(部分 -- ,完整 -- ,在主导上) 被识别,当在主导上的效果做了主要贡献到杂种优势时。这些结果可以帮助为在棉花澄清杂种优势的遗传机制打了基础。 | Xian Guo Yuping Guo Jun Ma Fang Wang Mizhen Sun Lijuan Gui Jiajia Zhou Xianliang Song Xuezhen Sun Tianzhen Zhang | 2013 | Journal of Integrative Plant Biology2013，55，8： | 13 |
| 12 | 水稻千粒重基因TGW6功能标记的开发与利用显示文摘水稻粒重是影响产量及品质的重要性状之一,TGW6是能显著增加千粒重同时不影响稻米品质的粒重基因。为提高千粒重基因TGW6在育种中的选择效率,在前人克隆的基础上,根据TGW6与其等位基因tgw6在功能区域存在的单碱基缺失,设计和筛选出TGW6基因的功能标记CAPs6-1,对CAPs6-1扩增的PCR产物测序验证,说明CAPs6-1可以准确鉴定出TGW6的不同基因型。利用该标记对不同来源的43份籼稻资源、江苏2007-2013年审定的65份粳稻品种和太湖流域179份粳稻地方资源进行了TGW6基因型检测,筛选到携带TGW6基因的籼稻资源14份,244份粳型资源均不携带TGW6基因,这也说明TGW6主要分布在籼型水稻资源中,在粳型资源中几乎不存在。 | 王军 杨杰 徐祥 朱金燕 范方军 李文奇 王芳权 仲维功 | 2014 | 中国水稻科学2014，28，5： | 13 |
| 13 | 水稻产量数量性状的遗传调控机制研究进展显示文摘粒重、每穗粒数和每株穗数是决定水稻产量的三大要素,也是水稻育种改良的重点.这些性状都是遗传复杂的数量性状.近十年来,水稻数量性状遗传学领域取得了突破性的进展,成功克隆了一批控制水稻产量性状的数量性状位点(QTL).本文将简要介绍产量性状相关QTL的功能与作用机制.这些研究成果不仅有助于揭示产量性状形成的遗传基础,也将有力推动水稻分子设计育种的进程. | 高继平 祁澎 林鸿宣 | 2013 | 中国科学:生命科学2013，43，12： | 10 |
| 14 | 水稻重要农艺性状自然变异研究进展及其应用策略显示文摘水稻在长期的自然进化和人工选择过程中积累了大量自然变异,这些自然变异是许多重要农艺性状的遗传基础,也是水稻育种筛选的主要目标.近些年,随着分子技术的发展,水稻自然变异研究取得了一系列重要突破.本文结合最新研究进展,系统介绍了水稻自然变异的特点及相应分析方法,并强调了高通量测序技术在今后水稻自然变异挖掘中的意义;对最近10年来报道的抽穗期、产量元件及抗逆和驯化相关性状自然变异的分子机制及驯化特征进行了详细说明;结合这些自然变异的分子特征,总结了利用不同变异类型改良现有品种的方法,为今后分子育种提供理论依据. | 张林 何祖华 | 2015 | 科学通报2015，60，12： | 10 |
| 15 | Exploration of rice yield potential: Decoding agronomic and physiological traits显示文摘Rice grain yield is determined by three major'visible'morphological traits:grain weight,grain number per panicle,and effective tiller number,which are affected by a series of'invisible'physiological factors including nutrient use efficiency and photosynthetic efficiency.In the past few decades,substantial progress has been made on elucidating the molecular mechanisms underlying grain yield formation,laying a solid foundation for improving rice yield by molecular breeding.This review outlines our current understanding of the three morphological yield-determining components and summarizes major progress in decoding physiological traits such as nutrient use efficiency and photosynthetic efficiency.It also discusses the integration of current knowledge about yield formation and crop improvement strategies including genome editing with conventional and molecular breeding. | Gengmi Li Jiuyou Tang Jiakui Zheng Chengcai Chu | 2021 | The Crop Journal2021，9，3： | 9 |
| 16 | 水稻粒长基因GS3和qGL3功能标记的设计及应用显示文摘水稻粒型是与其产量直接相关的重要性状。在前期研究证实来源于特大粒水稻TD70和籼稻品种Kasalath的重组自交系(RIL)群体中检测到已克隆的粒长GS3和q GL3基因的基础上,通过TD70和Kasalath的GS3、q GL3基因序列差异分析,设计功能标记,检测240个RILs和不同粒长的6个粳稻、9个籼稻中GS3、q GL3基因类型及其效应。结果显示:TD70和Kasalath中的GS3基因和q GL3基因分别在第2外显子上的编码区+165位置和第10外显子上的编码区+1 092位置存在一个由碱基C到碱基A的单核苷酸替换,据此设计了四引物扩增受阻突变体系(Tetra-primer ARMS-PCR)标记,TD70的GS3和q GL3基因分别扩增为270 bp和145 bp条带及448 bp和288bp条带,Kasalath的GS3、q GL3基因分别扩增为270 bp和175 bp条带及448 bp和216 bp条带;240个RILs中含有GS3-T和q GL3-T基因的株系61个,含GS3-T和q GL3-K的株系48个,含GS3-K和q GL3-T的株系17个;不同粒型基因组合的粒长存在显著差异,表现为GS3-T+q GL3-T>GS3-T+q GL3-K或GS3-K+q GL3-T>GS3-K+q GL3-K;长粒型的1个粳稻和5个籼稻品种的GS3基因表现为TD70带型,短粒型的5个粳稻和4个籼稻品种的GS3基因表现为Kasalath带型;所有水稻品种的q GL3基因均表现为Kasalath带型。表明开发的GS3和q GL3基因功能标记是有效的,可用于水稻分子标记辅助育种。 | 丁丹 张亚东 郑佳 赵春芳 陈涛 赵庆勇 朱镇 周丽慧 姚姝 赵凌 于新 王才林 | 2014 | 江苏农业学报2014，30，6： | 8 |
| 17 | A novel miR167a-OsARF6-OsAUX3 module regulates grain length and weight in rice显示文摘Grain size is one of the most important factors that control rice yield,as it is associated with grain weight(GW).To date,dozens of rice genes that regulate grain size have been isolated;however,the regulatory mechanism underlying GW control is not fully understood.Here,the quantitative trait locus qGL5 for grain length(GL)and GW was identified in recombinant inbred lines of 9311 and Nipponbare(NPB)and fine mapped to a candidate gene,OsAUX3.Sequence variations between 9311 and NPB in the OsAUX3 promoter and loss of function of OsAUX3 led to higher GL and GW.RNA sequencing,gene expression quantification,dual-luciferase reporter assays,chromatin immunoprecipitation-quantitative PCR,and yeast one-hybrid assays demonstrated that OsARF6 is an upstream transcription factor regulating the expression of OsAUX3.OsARF6 binds directly to the auxin response elements of the OsAUX3 promoter,covering a single-nucleotide polymorphism site between 9311 and NPB/Dongjin/Hwayoung,and thereby controls GL by altering longitudinal expansion and auxin distribution/content in glume cells.Furthermore,we showed that miR167a positively regulate GL and GW by directing OsARF6 mRNA silencing.Taken together,our study reveals that a novel miR167a-OsARF6-OsAUX3 module regulates GL and GW in rice,providing a potential target for the improvement of rice yield. | Jiyue Qiao Hongzhen Jiang Yuqing Lin Lianguang Shang Mei Wang Dongming Li Xiangdong Fu Markus Geisler Yanhua Qi Zhenyu Gao Qian Qian | 2021 | Molecular Plant2021，14，10： | 8 |
| 18 | 水稻粒宽基因GS5的功能标记开发和单倍型鉴定显示文摘水稻粒宽是影响籽粒粒形的重要因素之一,也是一个与水稻产量和稻米品质密切相关的重要性状。在基因GS5序列分析的基础上,对该基因第2外显子的ACC/CTA和第9外显子A/C的两个变异位点分别开发了功能标记,并将其用于294份水稻微核心种质和2007-2013年江苏省审定的65份粳稻品种的基因型鉴定。研究结果表明,这两个变异位点的等位变异在水稻籽粒的粒长、粒宽和长宽比性状上存在显著或极显著的差异;其在水稻微核心种质中组成的4种单倍型在水稻籼亚种的粒宽、粒厚和长宽比性状上存在极显著的差异,在粳亚种的粒宽和长宽比性状上存在极显著的差异;而江苏省审定的粳稻品种中仅发现Hap1和Hap2两种单倍型,其分别有64个和1个代表性品种。这些研究结果为水稻产量和稻米品质育种中充分利用GS5的优异等位基因或单倍型奠定了基础。 | 裔传灯 王德荣 蒋伟 李玮 成晓俊 王颖 周勇 梁国华 顾铭洪 | 2016 | 中国水稻科学2016，30，5： | 7 |
| 19 | QTL Mapping for Cucumber Fruit Size and Shape with Populations from Long and Round Fruited Inbred Lines显示文摘To gain insight into the molecular and genetic basis of fruit size and shape in cucumber,we conducted Quantitative Trait Locus(QTL)mapping with F2 and BC1F1 populations derived froma cross between the Northern-China type inbred line CNS21 and the Southern-China type inbred line RNS7.Populations were evaluated during two developmental stages,ovary at anthesis and commercial fruit(immature fruit).A total of 13 major-effect QTLs(R2>10%)were detected for six traits,and one of them,fruit shape index 2.1,explained more than 50%of phenotypic variation.All QTLs distributed on chromosome(chr)1 and 2.We constructed a physical map containing almost all of the QTLs with their physical location from previous studies.For fruit size and shape,the highest number of QTLs were mapped on chr1 and chr6,and the fewest number were mapped on chr2 and chr7.At least one consensus region was presented on each chromosome.In addition,three candidate genes were predicted between the long-fruited and round-fruited inbred lines by comparing the sequences of the consensus region of chr3,where many QTLs for marketable fruit length had been detected.Our results provide a base for additional QTLs and molecular markers for fruit size and shape in cucumber breeding. | Zhihui Gao Haiyang Zhang Chenxing Cao Jing Han Hao Li Zhonghai Ren | 2020 | Horticultural Plant Journal2020，6，3： | 7 |
| 20 | 基于PARMS技术的水稻粒形基因GW8分子标记的开发显示文摘【目的】开发水稻粒宽调控基因GW8的荧光分子标记,为快速、准确鉴定其基因型提供技术支持。【方法】通过与宽粒亲本GW8基因序列进行比对可知,窄粒杂交稻亲本美B的GW8基因序列在第二内含子有2个碱基缺失差异的特性之一,据此结合五引物扩增受阻突变体系技术,建立GW8基因荧光分子标记PM-GW8。【结果】利用荧光分子标记PM-GW8对42份籼稻亲本材料进行鉴定,仅在美B中检测到有2个碱基缺失的荧光信号。对亲本材料的谷粒宽度进行测量,其中美B的粒宽相对其他品种较窄,验证了该分子标记检测GW8的基因型与粒宽表型相符合。【结论】GW8荧光分子标记PM-GW8能快速检测水稻是否存在2个碱基缺失的GW8基因,为利用分子标记辅助选择改良水稻粒宽提供高效、可靠的基因分型技术。 | 卿冬进 刘开强 邓国富 高利军 黄娟 高菊 伍豪 戴高兴 梁海福 周维永 | 2019 | 西南农业学报2019，32，3： | 7 |
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