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| 1 | A Kelch Motif-Containing Serine/Threonine Protein Phosphatase Determines the Large Grain QTL Trait in Rice显示文摘A thorough understanding of the genetic basis of rice grain traits is critical for the improvement of rice (Oryza sativa L.) varieties.In this study,we generated an F 2 population by crossing the large-grain japonica cultivar CW23 with Peiai 64 (PA64),an elite indica small-grain cultivar.Using QTL analysis,17 QTLs for five grain traits were detected on four different chromosomes.Eight of the QTLs were newly-identified in this study.In particular,qGL3-1,a newly-identified grain length QTL with the highest LOD value and largest phenotypic variation,was fine-mapped to the 17 kb region of chromosome 3.A serine/threonine protein phosphatase gene encoding a repeat domain containing two Kelch motifs was identified as the unique candidate gene corresponding to this QTL.A comparison of PA64 and CW23 sequences revealed a single nucleotide substitution (C→A) at position 1092 in exon 10,resulting in replacement of Asp (D) in PA64 with Glu (E) in CW23 for the 364th amino acid.This variation is located at the D position of the conserved sequence motif AVLDT of the Kelch repeat.Genetic analysis of a near-isogenic line (NIL) for qGL3-1 revealed that the allele qGL3-1 from CW23 has an additive or partly dominant effect,and is suitable for use in molecular marker-assisted selection. | Zejun Hu Haohua He Shiyong Zhang Fan Sun Xiaoyun Xin Wenxiang Wang Xi Qian Jingshui Yang Xiaojin Luo | 2012 | Journal of Integrative Plant Biology2012，54，12： | 41 |
| 2 | OstMAPKKK5, a truncated mitogen-activated protein kinase kinase kinase 5, positively regulates plant height and yield in rice显示文摘Rice(Oryza sativa L.)is a major food crop worldwide.Plant height and yield are important agronomic traits of rice.Several genes regulating plant height and/or yield have been cloned.However,the molecular mechanisms coordinating plant height and yield are not fully characterized.Here,we report a novel gene,OstMAPKKK5 that encodes a truncated variant of a mitogen-activated protein kinase kinase kinase 5(OsMAPKKK5)lacking an intact kinase domain.Transgenic plants overexpressing OstMAPKKK5 in indica cultivar 9311 showed increased plant height,grain length,grain width,1000-grain weight,grain number per main panicle,and yield.Real-time quantitative PCR showed that OstMAPKKK5 was widely expressed in various tissues and developmental stages.The increased plant height and yield were attributed to enlarged cell size.Overexpression of OstMAPKKK5 led to higher contents of various forms of endogenous gibberellin(GA),especially the most common active forms,GA1,GA3,GA4.We concluded that OstMAPKKK5 positively regulates plant height and yield in rice by affecting cell size,and that its underlying mechanism is based on increased endogenous GA content. | Yahui Liu Yu Zhu Xuding Xu Fan Sun Jingshui Yang Liming Cao Xiaojin Luo | 2019 | The Crop Journal2019，7，5： | 2 |
| 3 | Characteristics of Focal Mechanism in Guangdong and Its Adjacent Areas显示文摘The focal mechanisms of 32 earthquakes with magnitude of M_S≥3.0, which were recorded by the Guangdong Digital Seismographic Network, are calculated by using P-wave polarity and amplitude ratio data, and those of 83 earthquakes of Guangdong and its adjacent areas derived by previous researchers are sorted out. On the basis of this, the characteristics of focal mechanism in Guangdong and its adjacent areas are summarized. Meanwhile the focal mechanisms of three earthquake areas, i.e., Yangjiang, Xinfengjiang and Nanao, are discussed respectively. Results show clear regional feature in the function and nature of the stress, and earthquakes are mostly of strike-slip associated with dip-slip component. Earthquakes in the Xinfengjiang Reservoir area are all reservoir-induced ones, so they are only of strike-slip and normal faulting types. In the Yangjiang area, some thrust earthquakes occurred. The dominant orientation of principal compressive stress axis changes from NS in Hainan, Gaungxi to NWW in Guangdong and finally near EW in the coastal areas of Guangdong and Fujian Provinces. | Kang Ying Yang Xuan Lu Jingshui Chen Xing Chen Guimei | 2006 | Earthquake Research in China2006，20，1： | 0 |
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