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| 1 | Rational design of three-phase interfaces for electrocatalysis显示文摘Gas-involving electrochemical reactions,like oxygen reduction reaction (ORR),oxygen evolution reaction (OER),and hydrogen evolution reaction (HER),are critical processes for energy-saving,environment-friendly energy conversion and storage technologies which gain increasing attention.The development of according electrocatalysts is key to boost their electrocatalytic performances.Dramatic efforts have been put into the development of advanced electrocatalysts to overcome sluggish kinetics.On the other hand,the electrode interfaces-architecture construction plays an equally important role for practical applications because these imperative electrode reactions generally proceed at triple-phase interfaces of gas,liquid electrolyte,and solid electrocatalyst.A desirable architecture should facilitate the complicate reactions occur at the triple-phase interfaces,which including mass diffusion,surface reaction and electron transfer.In this review,we will summarize some design principles and synthetic strategies for optimizing triple-phase interfaces of gas-involving electrocatalysis systematically,based on the electrode reaction process at the three-phase interfaces.It can be divided into three main optimization directions:exposure of active sites,promotion of mass diffusion and acceleration of electron transfer.Furthermore,we especially highlight several remarkable works with comprehensive optimization about specific energy conversion devices,including metal-air batteries,fuel cells,and water-splitting devices are demonstrated with superb efficiency.In the last section,the perspectives and challenges in the future are proposed. | Yuqing Wang Yuqin Zou Li Tao Yanyong Wang Gen Huang Shiqian Du Shuangyin Wang | 2019 | Nano Research2019，12，9： | 10 |
| 2 | MAP4K4 induces early blood-brain barrier damage in a murine subarachnoid hemorrhage model显示文摘Sterile-20-like mitogen-activated protein kinase kinase kinase kinase 4(MAP4 K4)is expressed in endothelial cells and activates inflammatory vascular damage.Endothelial cells are important components of the blood-brain barrier.To investigate whether MAP4 K4 plays a role in the pathophysiology of subarachnoid hemorrhage,we evaluated the time-course expression of MAP4 K4 after subarachnoid hemorrhage.A subarachnoid hemorrhage model was established using the intravascular perforation method.The model mice were assigned to four groups:MAP4 K4 recombinant protein,scramble small interfering RNA,and MAP4 K4 small interfering RNA were delivered by intracerebroventricular injection,while PF-06260933,a small-molecule inhibitor of MAP4 K4,was administrated orally.Neurological score assessments,brain water assessments,Evans blue extravasation,immunofluorescence,western blot assay,and gelatin zymography were performed to analyze neurological outcomes and mechanisms of vascular damage.MAP4 K4 expression was elevated in the cortex at 24 hours after subarachnoid hemorrhage,and colocalized with endothelial markers.MAP4 K4 recombinant protein aggravated neurological impairment,brain edema,and blood-brain barrier damage;upregulated the expression of phosphorylated nuclear factor kappa B(p-p65)and matrix metalloproteinase 9(MMP9);and degraded tight junction proteins(ZO-1 and claudin 5).Injection with MAP4 K4 small interfering RNA reversed these effects.Furthermore,administration of the MAP4 K4 inhibitor PF-06260933 reduced blood-brain barrier damage in mice,promoted the recovery of neurological function,and reduced p-p65 and MMP9 protein expression.Taken together,the results further illustrate that MAP4 K4 causes early blood-brain barrier damage after subarachnoid hemorrhage.The mechanism can be confirmed by inhibiting the MAP4 K4/NF-κB/MMP9 pathway.All experimental procedures and protocols were approved by the Experimental Animal Ethics Committee of General Hospital of Northern Theater Command(No.2018002)on January 15,2018. | Zheng Zou Yu-Shu Dong Dong-Dong Liu Gen Li Guang-Zhi Hao Xu Gao Peng-Yu Pan Guo-Biao Liang | 2021 | Neural Regeneration Research2021，16，2： | 5 |
| 3 | Preparation and Photovoltaic Properties of p-Type Nano-ZnFe_2O_4显示文摘p-Type nano-ZnFe2O4 semiconductors were gained by high-prssure treatment.Surface photovoltaic spectrum(SPS) and transient photovoltaic technology(TPV) were used for studying the photogenerated charge of nano-ZnFe2O4.Results show that the photovoltaic behavior of nano-ZnFe2O4 changed as the processing pressure increased.When the processing pressure was higher than 2 GPa,both SPS response interval and peak changed significantly.XPS results show that the non-lattice oxygen entered into the lattice and the content of lattice oxygen increased with the increase of processing pressure.The material changed from oxygen vacancy type to oxygen excess type and the photoelectric properties changed from n-type to p-type when the processing pressure is higher than 2 GPa. | LI Zi-heng ZOU Xu LI Gen ZOU Guang-tian | 2012 | Chemical Research in Chinese Universities2012，28，4： | 2 |
| 4 | Materials Lett显示文摘 | Wu Ju Zou Yuan - xi Mo Pei - gen | 1986 | 5:291986，5，： | 1 |
| 5 | Demethylbellidifolin Inhibits Proliferation and Activation ofHepatic Stellate Cells显示文摘 | Jun Fu Li Gen Fang Lu You Yi Zou | 2011 | Journal of Investigative Surgery2011，，4： | 1 |
| 6 | Analysis of genetic and epigenetic alterations of the PTEN gene in gastric cancer显示文摘 | Kiyoshi Sato Gen Tamura Takashi Tsuchiya Yasushi Endoh Ken Sakata Teiichi Motoyama Osamu Usuba Wataru Kimura Masanori Terashima Satoshi Nishizuka Tongtong Zou Stephen J. Meltzer | 2002 | Virchows Archiv2002，，2： | 1 |
| 7 | Improvement of cellulaseactivity in Trichoderma reesei by heterologous expression of a beta-glucosidase gene from Penicillium decumbens显示文摘 | MA Liang ZHANG Jun ZOU Gen | 2011 | Enzyme andMicrobial Technology2011，49，： | 1 |
| 8 | N ‐glycosylation affects the proper folding, enzymatic characteristics and production of a fungal β‐glucosidase显示文摘 | Wei Wei Ling Chen Gen Zou Qianfu Wang Xing Yan Jun Zhang Chengshu Wang Zhihua Zhou | 2013 | Biotechnol Bioeng2013，，12： | 1 |
| 9 | Analysis of genetic and epigenetic alterations of the PTEN gene in gastric cancer显示文摘 | Kiyoshi Sato Gen Tamura Takashi Tsuchiya Yasushi Endoh Ken Sakata Teiichi Motoyama Osamu Usuba Wataru Kimura Masanori Terashima Satoshi Nishizuka Tongtong Zou Stephen J. Meltzer | 2002 | Virchows Archiv2002，，2： | 1 |
| 10 | Ultrathin defective high-entropy layered double hydroxides for electrochemical water oxidation显示文摘One of the fundamental driving forces in the materials science community is the hunt for new materials with specific properties that meet the requirements of rapidly evolving technology. | Kaizhi Gu Xiaoyan Zhu Dongdong Wang Nana Zhang Gen Huang Wei Li Peng Long Jing Tian Yuqin Zou Yanyong Wang Ru Chen Shuangyin Wang | 2021 | Journal of Energy Chemistry2021，30，9： | 1 |
| 11 | Efficient genome editing in Claviceps purpurea using a CRISPR/Cas9 ribonucleoprotein method显示文摘Claviceps purpurea produces many pharmacologically important ergot alkaloids(EAS),which are widely used to treat migraine and hypertension and to aid childbirth.Although an EAS biosynthetic cluster of C.purpurea has been discovered more than 20 years ago,the complete biosynthetic pathway of EAS has not been fully characterized until now.The main obstacle to elucidating this pathway and strain modification is the lack of efficient genome-editing tools for C.purpurea.The conventional gene manipulation method for C.purpurea relies on homologous recombination(HR),although the efficiency of HR in C.purpurea is very low(~1-5%).Consequently,the disruption of target genes is laborious and time-consuming.Although CRISPR/Cas9 genome-editing methods based on in vivo Cas9 expression and gRNA transcription have been reported recently,their gene-disruption efficiency is still very low.Here,we developed an efficient genome-editing system in C.purpurea based on in vitro assembled CRISPR/Cas9 gRNA ribonucleoprotein complexes.As proof of principle,three target genes were efficiently knocked out using this CRISPR/Cas9 ribonucleoprotein complex-mediated HR system,with editing efficiencies ranging from 50%to 100%.Inactivation of the three genes,which are closely related to uridine biosynthesis(ura5),hypha morphology(rac),and EAS production(easA),resulted in a uridine auxotrophic mutant,a mutant with a drastically different phenotype in axenic culture,and a mutant that did not produce EAS,respectively.Our ribonucleoprotein-based genome-editing system has a great advantage over conventional and in vivo CRISPR/Cas9 methods for genome editing in C.purpurea,which will greatly facilitate elucidation of the EAS biosynthetic pathway and other future basic and applied research on C.purpurea. | Lu Yu Meili Xiao Zhihua Zhu Yinmei Wang Zhihua Zhou Pingping Wang Gen Zou | 2022 | Synthetic and Systems Biotechnology2022，7，2： | 1 |
| 12 | World Health Organization’s first-ever release of a fungal priority pathogens list:A reply action proposal for the prevention and treatment of fungal pathogens显示文摘Fungi are widely distributed in the environment,and some are beneficial to medicine,industry,agriculture,and food.For example,Penicillium chrysogenum,Acremonium chrysogenum,and Aspergillus terreus can synthesizeβ-lactams,which can be used as drugs[1];Aspergillus niger and Trichoderma reesei are well-known industrial enzyme producers[2]. | Gen Zou Yongjun Wei | 2023 | Eco-Environment & Health2023，2，2： | 0 |
| 13 | Lipid metabolism dysfunction induced by age-dependent DNA methylation accelerates aging显示文摘Epigenetic alterations and metabolic dysfunction are two hallmarks of aging.However,the mechanism of how their interaction regulates aging,particularly in mammals,remains largely unknown.Here we show ELOVL fatty acid elongase 2(Elovl2),a gene whose epigenetic alterations are most highly correlated with age prediction,contributes to aging by regulating lipid metabolism.We applied artificial intelligence to predict the protein structure of ELOVL2 and the interaction with its substrate.Impaired Elovl2 function disturbs lipid synthesis with increased endoplasmic reticulum stress and mitochondrial dysfunction,leading to key aging phenotypes at both cellular and physiological level.Furthermore,restoration of mitochondrial activity can rescue age-related macular degeneration(AMD)phenotypes induced by Elovl2 deficiency in human retinal pigmental epithelial(RPE)cells;this indicates a conservative mechanism in both human and mouse.Taken together,we revealed an epigenetic-metabolism axis contributing to aging and illustrate the power of an AI-based approach in structure-function studies. | Xin Li Jiaqiang Wang LeYun Wang Yuanxu Gao Guihai Feng Gen Li Jun Zou Meixin Yu Yu Fei Li Chao Liu Xue Wei Yuan Ling Zhao Hong Ouyang Jian-Kang Zhu Wei Li Qi Zhou Kang Zhang | 2022 | Signal Transduction and Targeted Therapy2022，7，6： | 0 |
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