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263篇 您的检索式:作者名="Gen Li"
    题名 作者 年代 出处 被引量
1Effect of lipid on proliferation and activation of rat hepatic stellate cells(Ⅰ)显示文摘INTRODUCTIONDespitetheearlycontroversyovertheprimarycelularsourceofextracelularmatrixproteinsinliverfibrosis,compelinginvitro...LU Lun Gen, ZENG Min De, LI Ji Qiang, HUA Jing, FAN Jian Gao, FAN Zhu Ping and QIU De Kai 1998World Journal of Gastroenterology1998,4,6:18
2Parkinson’s disease in China:a forty-year growing track of bedside work显示文摘The number and health burden of Parkinson’s disease increase rapidly in China.It is estimated that China will have nearly half of the Parkinson’s disease population in the world in 2030.In this review,we present an overview of epidemiology and health economics status of Parkinson’s disease across China and discuss the risk factors of Parkinson’s disease and related complications.From the view of clinical research,we also discuss the current status of clinical trials,diagnostic biomarkers,treatment of Parkinson’s disease,tertiary network and post-occupation education in Chinese Parkinson’s disease clinics.Gen Li Jianfang Ma Shishuang Cui Yixi He Qin Xiao Jun Liu Shengdi Chen 2019Translational Neurodegeneration2019,8,1:16
3Generalized Empirical Likelihood Inference in Semiparametric Regression Model for Longitudinal Data显示文摘在这篇论文,我们为纵的数据认为半是参量的回归模型。由于在组以内的关联,为在模型的未知参数的一个概括实验木头可能性比率统计数值被介绍工作协变性矩阵建议。建议统计数值是 asymptotically 标准的,这被证明在一些合适的条件下面摆平 chi,并且因此,构造参数的信心区域能被用来。模拟研究被进行以范围精确性和信心间隔的平均长度把建议方法与概括最少的广场方法作比较。Gao Rong LI Ping TIAN Liu Gen XUE 2008Acta Mathematica Sinica,English Series2008,24,12:12
4A New Alkaloid from the Root of Isatis indigotica Fort显示文摘A E-2-[(3’-indole)cyanomethylene]-3-indolinone was isolated from the root of Isatis indigotica Fort. The structure elucidation and 1H, 13C NMR assignments were achieved by spectroscopic method.Wan Sheng CHEN*, Bin LI, Wei Dong ZHANG, Gen Jing YANG, Chuan Zhuo QIAO Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, Shanghai 200433 2001Chinese Chemical Letters2001,12,6:11
5How Work Organization Affects the Prevalence of WMSDs: A Case-control Study显示文摘Objective In this study, we aimed at exploring the association between work-related musculoskeletal disorders(WMSDs) and work organization based on a case-control study. Methods A total of 1938 workers who claimed to suffer from WMSDs were selected from Beijing, Henan, Hubei, and the Guangdong province. The control group consisted of 2009 workers employed in similar industries without severe disease or musculoskeletal discomforts. We used a modified version of the questionnaire developed by the NMQ and the DMQ to investigate individual and work-related factors. Results A total of 13 variables(P<0.1) were selected by the chi-square test and finally, 7 variables entered into the equation, with 6 variables reaching statistical significance(P<0.05). The odds ratios(OR) of ‘work changing with season' and ‘sufficient rest time' did not reach 1(0.749 and 0.441, respectively). In addition, ‘sufficient rest time' seemed to be the stronger protective factor according to its higher standardized coefficient. And ‘repetitive work every minute', ‘constantly repetitive work'(every day), ‘shortage of site personnel', and ‘often switching shifts with others' seemed to be the risk factors. Conclusion Work organization may have comprehensive effects on the occurrence of WMSDs. This pattern of associations suggests that further investigation into the mechanism of how work organization affects the prevalence of WMSDs is required.LIU Lu CHEN Song Gen TANG Shi Chuan WANG Sheng HE Li Hua GUO Ze Hua LI Jing Yun YU Shan Fa WANG Zhong Xu 2015Biomedical and Environmental Sciences2015,28,9:11
6Rational design of three-phase interfaces for electrocatalysis显示文摘Gas-involving electrochemical reactions,like oxygen reduction reaction (ORR),oxygen evolution reaction (OER),and hydrogen evolution reaction (HER),are critical processes for energy-saving,environment-friendly energy conversion and storage technologies which gain increasing attention.The development of according electrocatalysts is key to boost their electrocatalytic performances.Dramatic efforts have been put into the development of advanced electrocatalysts to overcome sluggish kinetics.On the other hand,the electrode interfaces-architecture construction plays an equally important role for practical applications because these imperative electrode reactions generally proceed at triple-phase interfaces of gas,liquid electrolyte,and solid electrocatalyst.A desirable architecture should facilitate the complicate reactions occur at the triple-phase interfaces,which including mass diffusion,surface reaction and electron transfer.In this review,we will summarize some design principles and synthetic strategies for optimizing triple-phase interfaces of gas-involving electrocatalysis systematically,based on the electrode reaction process at the three-phase interfaces.It can be divided into three main optimization directions:exposure of active sites,promotion of mass diffusion and acceleration of electron transfer.Furthermore,we especially highlight several remarkable works with comprehensive optimization about specific energy conversion devices,including metal-air batteries,fuel cells,and water-splitting devices are demonstrated with superb efficiency.In the last section,the perspectives and challenges in the future are proposed.Yuqing Wang Yuqin Zou Li Tao Yanyong Wang Gen Huang Shiqian Du Shuangyin Wang 2019Nano Research2019,12,9:10
7Updated diagnosis, treatment and prevention of COVID-19 in children: experts' consensus statement (condensed version of the second edition)显示文摘In the early February,2020,we called up an experts' committee with more than 30 Chinese experts from 11 national medical academic organizations to formulate the first edition of consensus statement on diagnosis,treatment and prevention of coronavirus disease 2019 (COVID-19) in children,which has been published in this journal.With accumulated experiences in the diagnosis and treatment of COVID-19 in children,we have updated the consensus statement and released the second edition recently.The current version in English is a condensed version of the second edition of consensus statement on diagnosis,treatment and prevention of COVID-19 in children.In the current version,diagnosis and treatement criteria have been optimized,and early identification of severe and critical cases is highlighted.The early warning indicators for severe pediatric cases have been summarized which is utmost important for clinical practice.This version of experts consensus will be valuable for better prevention,diagnosis and treatment of COVID-19 in children worldwide.Kun-Ling Shen Yong-Hong Yang Rong-Meng Jiang Tian-You Wang Dong-Chi Zhao Yi Jiang Xiao-Xia Lu Run-Ming Jin Yue-Jie Zheng Bao-Ping Xu Zheng-De Xie Zhi-sheng Liu Xing-wang Li Li-Kai Lin Yun-Xiao Shang Sai-Nan Shu Yan Bai Min Lu Gen Lu Ji-Kui Deng Wan-Jun Luo Li-Juan Xiong Miao Liu Yu-Xia Cui Le-Ping Ye Jia-Fu Li Jian-Bo Shao Li-Wei Gao Yong-Yan Wang Xue-Feng Wang 2020World Journal of Pediatrics2020,16,3:9
8TP53-induced glycolysis and apoptosis regulator alleviates hypoxia/ischemia-induced microglial pyroptosis and ischemic brain damage显示文摘Our previous studies have demonstrated that TP53-induced glycolysis and apoptosis regulator(TIGAR)can protect neurons after cerebral ischemia/reperfusion.However,the role of TIGAR in neonatal hypoxic-ischemic brain damage(HIBD)remains unknown.In the present study,7-day-old Sprague-Dawley rat models of HIBD were established by permanent occlusion of the left common carotid artery followed by 2-hour hypoxia.At 6 days before induction of HIBD,a lentiviral vector containing short hairpin RNA of either TIGAR or gasdermin D(LV-sh_TIGAR or LV-sh_GSDMD)was injected into the left lateral ventricle and striatum.Highly aggressively proliferating immortalized(HAPI)microglial cell models of in vitro HIBD were established by 2-hour oxygen/glucose deprivation followed by 24-hour reoxygenation.Three days before in vitro HIBD induction,HAPI microglial cells were transfected with LV-sh_TIGAR or LV-sh_GSDMD.Our results showed that TIGAR expression was increased in the neonatal rat cortex after HIBD and in HAPI microglial cells after oxygen/glucose deprivation/reoxygenation.Lentivirusmediated TIGAR knockdown in rats markedly worsened pyroptosis and brain damage after hypoxia/ischemia in vivo and in vitro.Application of exogenous nicotinamide adenine dinucleotide phosphate(NADPH)increased the NADPH level and the glutathione/oxidized glutathione ratio and decreased reactive oxygen species levels in HAPI microglial cells after oxygen/glucose deprivation/reoxygenation.Additionally,exogenous NADPH blocked the effects of TIGAR knockdown in neonatal HIBD in vivo and in vitro.These findings show that TIGAR can inhibit microglial pyroptosis and play a protective role in neonatal HIBD.The study was approved by the Animal Ethics Committee of Soochow University of China(approval No.2017LW003)in 2017.Lan-Lan Tan Xiao-Lu Jiang Li-Xiao Xu Gen Li Chen-Xi Feng Xin Ding Bin Sun Zheng-Hong Qin Zu-Bin Zhang Xing Feng Mei Li 2021Neural Regeneration Research2021,16,6:9
9Role of free fatty acids in proliferation of rat hepatic stellate cells(Ⅱ)显示文摘INTRODUCTIONAlthoughtheroleofhepatocytesinfatyacidsmetabolismandtransporthavebeendiscusedintensively,fewofthatofHSCwasreporte...LU Lun Gen, ZENG Min De, LI Ji Qiang, HUA Jing, FAN Jian Gao and QIU De Kai 1998World Journal of Gastroenterology1998,4,6:8
10Onset of Coronary Heart Disease is Associated with HCMV Infection and Increased CD14^+CD16^+Monocytes in a Population of Weifang,China显示文摘Objective To investigate the relationship between human cytomegalovirus(HCMV)infection and peripheral blood CD14+CD16+monocytes in the pathogenesis of coronary heart disease(CHD),and to elucidate the mechanism of pathogenesis in CHD by analyzing the correlation between infection,inflammation,and CHD,to provide a basis for the prevention,evaluation,and treatment of the disease.Methods In total,192 patients with CHD were divided into three groups:latent CHD,angina pectoris,and myocardial infarction.HCMV-IgM and-IgG antibodies were assessed using ELISA;CD14+CD16+monocytes were counted using a five-type automated hematology analyzer;mononuclear cells were assessed using fluorescence-activated cell sorting;and an automatic biochemical analyzer was used to measure the levels of triglyceride,cholesterol,high-and low-density lipoprotein cholesterols,lipoprotein,hs-CRp and Hcy.Results The positive rates of HCMV-IgM and-IgG were significantly higher in the CHD groups than in the control group.HCMV infection affects lipid metabolism to promote immune and inflammatory responses.Conclusion HCMV infection has a specific correlation with the occurrence and development of CHD.The expression of CD14+CD16+mononuclear cells in the CHD group was increased accordingly and correlated with acute HCMV infection.Thus,HCMV antibody as well as peripheral blood CD14+CD16+mononuclear cells can be used to monitor the occurrence and development of CHD.LI Hong Zheng WANG Qin ZHANG Yi Yuan WANG Jin Dong WU Hong Juan ZHANG Mo Gen LI Ji Chen LIU Zhi Jun 2020Biomedical and Environmental Sciences2020,33,8:8
11Traditional El Nio and El Ni o Modoki Revisited:Is El Nio Modoki Linearly Independent of Traditional El Nio?显示文摘The present study revisited the first two leading modes of tropical Pacific sea surface temperature anomalies (SSTA) during the period of 1979 2008.It is suggested that the so-called El Nio Modoki,which is captured by the second mode,exists objectively and exhibits obvious differences from traditional El Nio,which is captured by the first mode,in terms of its spatial characteristics.Furthermore,the authors found that El Nio Modoki is linearly independent of traditional El Nio;hence,it cannot be described as part of the traditional El Nio evolution,and vice versa.LI Gen REN Bao-Hua YANG Cheng-Yun ZHENG Jian-Qiu 2010Atmospheric and Oceanic Science Letters2010,3,2:7
12Soluble Nogo receptor 1 fusion protein protects neural progenitor cells in rats with ischemic stroke显示文摘Soluble Nogo66 receptor-Fc protein(sNgR-Fc)enhances axonal regeneration following central nervous system injury.However,the underlying mechanisms remain unclear.In this study,we investigated the effects of sNgR-Fc on the proliferation and differentiation of neural progenitor cells.The photothrombotic cortical injury model of ischemic stroke was produced in the parietal cortex of Sprague-Dawley rats.The rats with photothrombotic cortical injury were randomized to receive infusion of 400μg/kg sNgR-Fc(sNgR-Fc group)or an equal volume of phosphate-buffered saline(photothrombotic cortical injury group)into the lateral ventricle for 3 days.The effects of sNgR-Fc on the proliferation and differentiation of endogenous neural progenitor cells were examined using BrdU staining.Neurological function was evaluated with the Morris water maze test.To further examine the effects of sNgR-Fc treatment on neural progenitor cells,photothrombotic cortical injury was produced in another group of rats that received transplantation of neural progenitor cells from the hippocampus of embryonic Sprague-Dawley rats.The animals were then given an infusion of phosphate-buffered saline(neural progenitor cells group)or sNgR-Fc(sNgR-Fc+neural progenitor cells group)into the lateral ventricle for 3 days.sNgR-Fc enhanced the proliferation of cultured neural progenitor cells in vitro as well as that of endogenous neural progenitor cells in vivo,compared with phosphate-buffered saline,and it also induced the differentiation of neural progenitor cells into neurons.Compared with the photothrombotic cortical injury group,escape latency in the Morris water maze and neurological severity score were greatly reduced,and distance traveled in the target quadrant was considerably increased in the sNgR-Fc group,indicating a substantial improvement in neurological function.Furthermore,compared with phosphate-buffered saline infusion,sNgR-Fc infusion strikingly improved the survival and differentiation of grafted neural progenitor cells.Our findings show that sNgR-Fc regulates neural progenitor cell proliferation,migration and differentiation.Therefore,sNgR-Fc is a potential novel therapy for stroke and neurodegenerative diseases,The protocols were approved by the Committee on the Use of Live Animals in Teaching and Research of the University of Hong Kong(approval No.4560-17)in November,2015.Hai-Wei He Yue-Lin Zhang Bao-Qi Yu Gen Ye Wei You Kwok-fai So Xin Li 2019Neural Regeneration Research2019,14,10:6
13Relationship between expression p53 protein,PCNA and CEA in colorectal cancer and lymph node metastasis显示文摘Relationshipbetweenexpressionp53protein,PCNAandCEAincolorectalcancerandlymphnodemetastasisXUQingWen1,LIYanSong2andZHUHeGen...XU QingWen1, LI YanSong2 and ZHU HeGen11Department of Surgery, 2Department of Pathology, The Affiliated Hospital of Guangdong Medical College, Zhanjiang 524001, Guangdong Province, China 1998World Journal of Gastroenterology1998,4,3:6
14Hollow spherical rare-earth-doped yttrium oxysulfate: A novel structure for upconversion显示文摘锻烧跟随的一条灵巧的 帮助biomolecule 的热水的线路为空范围与另外的稀土元素的离子做了的单斜晶的钇 oxysulfate 的准备被采用了( Yb <啜class=“ a-plus-plus ”>3+和 Eu <啜class=“ a-plus-plus ”>3+或嗯<啜class=“ a-plus-plus ”>3+)。空范围的形成可以包含 Ostwald 成熟。产生混合材料被用于 upconversion 应用。主人水晶结构允许容易没有显著地改变主人格子,二个不同稀土元素的金属离子共同做。光性质被稀土元素的金属离子由于精力转移和水晶的对称回答的掺杂物的比率和集中影响。光中心的类型和样品的 crystallinity 也被显示在同样准备的产品的光性质上有重要影响。Gen Chen Fashen Chen Xiaohe Liu Wei Ma Hongmei Luo Junhui Li Renzhi Ma Guanzhou Qiu 2014Nano Research2014,7,8:6
15Selective conversion of syngas to propane over ZnCrO_x-SSZ-39 OX-ZEO catalysts显示文摘Oxide-Zeolite(OX-ZEO) bifunctional catalyst design concept has been exemplified in several processes to direct conversion syngas to value-added chemicals and fuels such as mixed light olefins, ethylene, aromatics and gasoline.Herein we demonstrate that the product can be steered toward liquefied petroleum gas(LPG) with a selectivity up to 89% in hydrocarbons especially propane selectivity reaching 80% at CO conversion of 63% using ZnCrOx-H-SSZ-39 catalyst.Interestingly, the quantity of the acid sites of SSZ-39 does not influence obviously the hydrocarbon distribution but the strength is crucial for selective formation of propane.This finding provides an alternative route of LPG synthesis from a variety of carbon resources via syngas.Gen Li Feng Jiao Dengyun Miao Yong Wang Xiulian Pan Toshiyuki Yokoi Xiangju Meng Feng-Shou Xiao Andrei-Nicolae Parvulescu Ulrich Müller Xinhe Bao 2019Journal of Energy Chemistry2019,28,9:6
16Transfer of mitochondria from mesenchymal stem cells derived from induced pluripotent stem cells attenuates hypoxia-ischemia-induced mitochondrial dysfunction in PC12 cells显示文摘Mitochondrial dysfunction in neurons has been implicated in hypoxia-ischemia-induced brain injury.Although mesenchymal stem cell therapy has emerged as a novel treatment for this pathology,the mechanisms are not fully understood.To address this issue,we first co-cultured 1.5×10^5 PC12 cells with mesenchymal stem cells that were derived from induced pluripotent stem cells at a ratio of 1:1,and then intervened with cobalt chloride(CoCl2)for 24 hours.Reactive oxygen species in PC12 cells was measured by Mito-sox.Mitochondrial membrane potential(ΔΨm)in PC12 cells was determined by JC-1 staining.Apoptosis of PC12 cells was detected by terminal deoxynucleotidal transferase-mediated dUTP nick end-labeling staining.Mitochondrial morphology in PC12 cells was examined by transmission electron microscopy.Transfer of mitochondria from the mesenchymal stem cells derived from induced pluripotent stem cells to damaged PC12 cells was measured by flow cytometry.Mesenchymal stem cells were induced from pluripotent stem cells by lentivirus infection containing green fluorescent protein in mitochondria.Then they were co-cultured with PC12 cells in Transwell chambers and treated with CoCl2 for 24 hours to detect adenosine triphosphate level in PC12 cells.CoCl2-induced PC12 cell damage was dose-dependent.Co-culture with mesenchymal stem cells significantly reduced apoptosis and restoredΔΨm in the injured PC12 cells under CoCl2 challenge.Co-culture with mesenchymal stem cells ameliorated mitochondrial swelling,the disappearance of cristae,and chromatin margination in the injured PC12 cells.After direct co-culture,mitochondrial transfer from the mesenchymal stem cells stem cells to PC12 cells was detected via formed tunneling nanotubes between these two types of cells.The transfer efficiency was greatly enhanced in the presence of CoCl2.More importantly,inhibition of tunneling nanotubes partially abrogated the beneficial effects of mesenchymal stem cells on CoCl2-induced PC12 cell injury.Mesenchymal stem cells reduced CoCl2-induced PC12 cell injury and these effects were in part due to efficacious mitochondrial transfer.Yan Yang Gen Ye Yue-Lin Zhang Hai-Wei He Bao-Qi Yu Yi-Mei Hong Wei You Xin Li 2020Neural Regeneration Research2020,15,3:6
17Ascent schedules,acute altitude illness,and altitude acclimatization:Observations on the Yushu Earthquake显示文摘During the Yushu Earthquake on April 14,2010,a large number of rescuers from sea level or lowlands ascended to the quake areas very rapidly or rapidly less than 24 h. However,Yushu Earthquake is the highest quake in the world at altitudes between 3 750 m and 4 878 m where is a serious hypoxic environment. A high incidence of acute altitude illness was found in the unacclimatized rescuers;the mountain rescue operation changed as 'rescue the rescuers'. Lesson from the Yushu Earthquake is that the occurrence of acute altitude illness may be closely related to the ascent schedules. This prompted us to study the relationship between ascent rate and the incidence and severity of acute altitude illness;five different groups were compared. The first group was 42 sea level male young soldiers who ascended to quake area very rapidly within 8 h at 4 000 m;the second group was 48 sea level male young soldiers who ascended to 4 000 m rapidly less than 18 h;the third group was 66 acclimatized medical workers from 2 261 m who ascended to 4 000 m rapidly within 12 h;the fourth group was 56 Tibetan medical workers from 2 800 m who ascended to 4 000 m rapidly within 8 h;the fifth group was 50 male sea level workers who ascended to 4 000 m gradually over a period of 4 d. The results showed that the sea level rescuers ascended to 4 000 m very rapidly or rapidly had the highest incidence of acute mountain sickness (AMS) with the greatest AMS scores and the lowest arterial oxygen saturation (SaO2);the sea level workers ascended to 4 000 m gradually had moderate incidence of AMS with moderate AMS scores and SaO2 values;whereas the acclimatized and adapted rescuers had the lowest incidence of AMS,lowest AMS scores and higher SaO2;especially none AMS occurred in Tibetan rescuers. AMS score is inversely related to the ascent rate (r=-0.24,p< 0.001). Additionally,acute altitude illness is significantly influenced by altitude acclimatization. The ascent rate is inversely re- lated to the period of altitude acclimatization whereas the time of perfect recovered from AMS is positively correlated to the time taken to acclimatize. Generally,the best means of preventing acute altitude illness is slow and gradual ascent to high altitude,as this allows time for establishing altitude acclimatization and tolerance to the hypoxic environment. However,during an emergency circumstance,such as mountain rescue operation,the rescuers must rapidly ascend to high altitude,so a series of preventive strategies including pre-acclimatization,using some prophylactic drugs and oxygen supplementary are sorely necessary.Wu Tianyi Hou Shike Li Shuzhi Li Wenxiang Gen Deng 2013Engineering Sciences2013,11,2:5
18A Study on Spatial Interpolation of Temperature in Anhui Province Based on ANUSPLIN显示文摘To achieve refined temperature grid data with high accuracy and high spatial resolution,hourly temperature grid dataset with spatial resolution of 1 km in Anhui Province from January to December in 2016 was established using the ANUSPLIN thin plate spline algorithm,which meets the needs of climate change research and meteorological disaster risk assessment. And the interpolation error was analyzed. The results show that the interpolated values of hourly temperature by ANUSPLIN are close to the observed values in 2016. The error is generally below 1. 5 ℃,and the root mean square error is 0. 937 6 ℃. On monthly scale,the interpolated values of hourly temperature by ANUSPLIN are also close to the observed values.In October,November,June and May,the interpolation accuracy is the highest,and the proportion of absolute error of hourly temperature lower than 2 ℃ is up to 99%,97. 4%,98. 1% and 97. 4% respectively. In February,March,August and December,the interpolation accuracy is the lowest,and the proportion of absolute error higher than 2 ℃ is 8. 1%,5. 3%,4. 1% and 4. 2% respectively. Due to the effect of complex topography in Anhui,the interpolation accuracy is the lowest in the mountainous areas of southern and western Anhui,and the interpolation error in these regions even exceeds 1. 5 ℃ annually and 1. 8 ℃ monthly.Wen Huayang Chen Fengjiao Li Jun Qiu Kangjun Wang Gen 2019Meteorological and Environmental Research2019,10,2:5
19A New Alkaloid from Patrinia scabra显示文摘A new alkaloid has been isolated from the root of Patrinia scabra. Its structure was elucidated as 2-acetamido-3-phenyl propyl 2-benzamido-3-phenyl propionate by extensive spectr- oscopic analysis.Zheng Bing GU*, Gen Jin YANG, Wen Yong LIU, Ting Zhao LI, Yan QIU, Wei Dong ZHANG College of Pharmacy, Second Military Medical University, Shanghai 200433 2002Chinese Chemical Letters2002,13,10:5
20A New Glycoside from Erigeron Breviscapus显示文摘Erigeside I was isolated from Erigeron breviscapus. The structure elucidation and 1H.13C NMR assignments were achieved by spectroscopic method.Wei Dong ZHANG De Yun KONG Hui Ting LI Gen Jin YANG(Department of Traditional Chinese Medicine. Shanghai Institute ofPharmaceutical Industry. 1320 Beijing Road (w.), Shanghai 200040) 1999Chinese Chemical Letters1999,10,2:5
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