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| 1 | Protein-Protein Interactions in the Regulation of WRKY Transcription Factors显示文摘自从一个 WRKY 抄写因素的第一份报告,现在是几乎 20 年, SPF1,从香甜的土豆。大进步从那以后在在植物生长,开发,和对关於生命、不能生活的应力的回答建立 WRKY 抄写因素的多样的生物角色被取得了。尽管有功能的差异,几乎所有分析 WRKY 蛋白质认出 TTGACC/T W 盒子序列并且,因此除核心 W 盒子倡导者元素的仅仅的识别以外的机制是必要的完成 WRKY 抄写因素的规章的特性。在过去的几年的研究表明了 WRKY 抄写因素身体上在发信号,抄写,并且染色质改变与角色与大量蛋白质交往。交往 WRKY 蛋白质的研究提供了重要卓见进抄写因素的重要家庭的成员的行动的规定和模式。稍微改变的 WRKY 领域和在每七个 WRKY 亚科以内保存的另外的蛋白质主题参予 proteinprotein 相互作用并且调停,这也出现了在 WRKY 蛋白质之间并且在在重要生物过程的调整的 WRKY 和另外的规章的蛋白质之间的复杂功能的相互作用。在这评论,我们为 WRKY 抄写因素总结 proteinprotein 相互作用的研究并且讨论交往的搭挡怎么作出贡献,到 WRKY 抄写因素的复杂规章、功能的网络的建立,在不同层次。 | Yingjun Chi Yan Yang Yuan Zhou Jie Zhou Baofang Fan Jing-Quan Yu Zhixiang Chen | 2013 | Molecular Plant2013，6，2： | 39 |
| 2 | Potentiating CD8^(+) T cell antitumor activity by inhibiting PCSK9 to promote LDLR-mediated TCR recycling and signaling显示文摘Metabolic regulation has been proven to play a critical role in T cell antitumor immunity.However,cholesterol metabolism as a key component of this regulation remains largely unexplored.Herein,we found that the low-density lipoprotein receptor(LDLR),which has been previously identified as a transporter for cholesterol,plays a pivotal role in regulating CD8+T cell antitumor activity.Besides the involvement of cholesterol uptake which is mediated by LDLR in T cell priming and clonal expansion,we also found a non-canonical function of LDLR in CD8+T cells:LDLR interacts with the T-cell receptor(TCR)complex and regulates TCR recycling and signaling,thus facilitating the effector function of cytotoxic T-lymphocytes(CTLs).Furthermore,we found that the tumor microenvironment(TME)downregulates CD8+T cell LDLR level and TCR signaling via tumor cell-derived proprotein convertase subtilisin/kexin type 9(PCSK9)which binds to LDLR and prevents the recycling of LDLR and TCR to the plasma membrane thus inhibits the effector function of CTLs.Moreover,genetic deletion or pharmacological inhibition of PCSK9 in tumor cells can enhance the antitumor activity of CD8+T cells by alleviating the suppressive effect on CD8+T cells and consequently inhibit tumor progression.While previously established as a hypercholesterolemia target,this study highlights PCSK9/LDLR as a potential target for cancer immunotherapy as well. | Juanjuan Yuan Ting Cai Xiaojun Zheng Yangzi Ren Jingwen Qi Xiaofei Lu Huihui Chen Huizhen Lin Zijie Chen Mengnan Liu Shangwen He Qijun Chen Siyang Feng Yingjun Wu Zhenhai Zhang Yanqing Ding Wei Yang | 2021 | Protein & Cell2021，12，4： | 12 |
| 3 | Preparation and evaluation of biomimetric nano-hydroxyapatite-based composite scaffolds for bone-tissue engineering显示文摘In the present study,novel biomimetic composite scaffolds with a composition similar to that of natural bone were prepared,using nano-hydroxyapatite,collagen,and phosphatidylserine.The scaffolds possess an interconnected porous structure with a porosity of 84%.The pore size ranges from several micrometers up to about 400 m.In-vitro studies in simulated body fluids showed that the morphologies of the products derived from mineralization can be regulated by the extracellular matrix components of the scaffolds;this in turn leads to creation of a large number of hydroxyapatite crystals on the scaffold surface.The regulatory properties of collagen and phosphatidylserine also influenced the cell response to the composite scaffolds.MC3T3-E1 cells attached and spread on the surfaces of the materials and interacted with the substrates;this may be the result of charged groups on the composite materials.Radiological analysis suggested that calluses and bone bridges formed in defects within 12 weeks.These composite scaffolds may therefore be a suitable replacement in bone-tissue engineering. | YANG ChunRong WANG YingJun CHEN XiaoFeng | 2012 | Chinese Science Bulletin2012，57，21： | 9 |
| 4 | Tribenuron-Methyl Induces Male Sterility through Anther-Specific Inhibition of Acetolactate Synthase Leading to Autophagic Cell Death显示文摘Tribenuron 甲基(TM ) 是禁止分支链的强大的 sulfonylurea 除草剂由指向 acetolactate synthase (ALS ) 的催化子单元(CSR1 ) 的氨基酸(BCAA ) 生合成。由在低剂量的 TM 的叶的喷洒的男绝育的选择正式就职广泛地在油菜籽(Brassica 马来麝) 为混合种子生产被使用了;然而,内在的机制仍然保持未知。这里,我们 reportgreater TM 累积和在花药的 BCAA 饥饿比在在 TM 申请以后的叶子和茎。csr1-1D (aCSR1 异种) 的组成或花药特定的表示消除了花药选择的 ALS 抑制并且在油菜籽和 Arabidopsis 颠倒了导致 TM 的男无菌的显型。TM 涂抹茎实验的结果,在叶肉或韧皮部由 TM 新陈代谢基因 Bel 的指向的表示在导致 TM 的男绝育的花药和回复与很少 TM 累积的观察结合了,建议叶水花的 TM 通过叶肉和韧皮部主要对花药极运输。显微镜学和 immunoblotting 揭示了那 autophagy,在房间死亡期间导致的一个体积降级过程,在导致 TM 的男无菌的花药并且由被提高花药特定 ALS 击倒。而且,导致 TM 的花粉流产被 autophagy 禁止者 3 麻省显著地禁止。这些数据最终建议 TM 对花药极运输,导致经由花药特定的 ALS 抑制的 BCAA 饥饿并且,,在花药的 autophagic 房间死亡。 | Lun Zhao Xue Jing Li Chen Yingjun Liu Yanan Su Tingting Liu Changbin Gao Bin Yi Jing Wen Chaozhi Ma Jinxing Tu Jitao Zou Tingdong Fu Jinxiong Shen | 2015 | Molecular Plant2015，8，12： | 7 |
| 5 | Improved clinical outcomes of rhG-CSF-mobilized blood and marrow haploidentical transplantation compared to propensity score-matched rhG-CSF-primed peripheral blood stem cell haploidentical transplantation:a multicenter study显示文摘The effects of haploidentical rhG-CSF-mobilized blood and marrow transplantation(HBMT) on hematological malignances are well established. Previous prospective single-center studies have demonstrated better survival after HBMT versus haploidentical rhG-CSF-mobilized peripheral blood stem cell transplantation(HPBSCT) for acute leukemia(AL) not in remission(NR) or in more than the second complete remission(>CR2). To test the hypothesis that HBMT is still superior to HPBSCT for patients with AL, multiple myeloma(MM), or non-Hodgkin lymphoma(NHL) in CR1/CR2 and for patients with chronic myeloid leukemia in the first and second chronic phase lacking a matched donor, we designed a propensity score method-based multicenter study.Hematopoietic recovery, acute graft-versus-host disease(aGVHD), and chronic GVHD were comparable between the HBMT group(n=168) and the HPBSCT group(n=42). No significant differences were found in non-relapse mortality rate(20.17%±3.58%and 27.24%±7.16%, P=0.18) or relapse rate(19.96%±3.72% and 28.49%±8.25%, P=0.32) between the HBMT group and the HPBSCT group. HBMT recipients had better overall survival(65.0%±4.2% and 54.2%±8.3%, P=0.037) and disease-free survival(59.9%±4.6% and 44.3%±8.7%, P=0.051). Multivariate analysis showed that HPBSCT was associated with poorer DFS(HR(95%CI), 1.639(0.995–2.699), P=0.052). Our comparisons showed that HBMT was superior to HPBSCT as a post-remission treatment for patients lacking an identical donor. | Xiangyu Zhao Feng Gao Xiaohui Zhang Yu Wang Lanping Xu Kaiyan Liu Xiaosu Zhao Yingjun Chang Han Wei Huan Chen Yuhong Chen Zhengfan Jiang Xiaojun Huang | 2016 | Science China(Life Sciences)2016，59，11： | 7 |
| 6 | Response of Pinus taiwanensis growth to climate changes at its southern limit of Daiyun Mountain, China's Mainland Fujian Province显示文摘The Pinus taiwanensis trees of a Chinese endemic species form pure forests at infertile sites in humid subtropical China, which can aid soil reservation and ecological resiliency at such sites. Herein, we used dendrochronological methods to investigate the growth patterns and their relationship with climate by analyzing 158 cores of 79 P. taiwanensis trees at 4 sites in their southernmost distribution in China's Mainland at Daiyun Mountain in Fujian Province. Tree growths at sites with favorable conditions generally showed an age-related growth trend, which decreased from approximately 5 mm to 0.5 mm in about a century. Trees in moderately stressed environments established high growth in their juvenile periods but were highly susceptible to environmental stresses such as a sharp growth decline in the 1990 s. The temperature in February and the moisture in July are the major limiting factors for most of the tree growths, except for a few extremely stressed P. taiwanensis trees. The growth of the dwarf P. taiwanensis trees, with all ring diameters of the first 20 years less than 4 mm, is mainly limited by the dry climate in May. Under the potential future warming trend, drought stress can be particularly threatening to these dwarf P. taiwanensis trees, which are likely to be the germplasm resources for this species in this region. | CHEN Dan FANG KeYan LI YingJun DONG ZhiPeng ZHANG Yu ZHOU FeiFei | 2016 | Science China Earth Sciences2016，59，2： | 6 |
| 7 | Seasonal variations of carboxylic acids and their contributions to the rainwater acidity: A case study of Guiyang and Shangzhong,China显示文摘Low molecule weight carboxylic acids are ubiquitous and important chemical constituents in the troposphere.Seven carboxylic acids in the rainwater of Guiyang and Shangzhong were simultaneously determined by ion chromatography from April 2006 to April 2007.Formic, acetic and oxalic acids were found to be the predominant carboxylic acids.Their volume weighted average concentration (VWA) in the rainwater of Guiyang were 14.24 μmol/L, 9.35 μmol/L and 2.79 μmol/L, respectively;as compared to 4.95 μmol/L, 1.35 μmol/L and 2.31 μmol/L in the rainwater of Shangzhong.In Shangzhong it is witnessed that the concentra-tions of these acids were higher in the summer than in the winter and direct emissions from vegetations or soils may account for the main provenance of the acids.This is, however, not the case in Guiyang, where the concentrations of the carboxylic acids were higher during the non-growing season than during the growing season.The relatively weak scavenging affected by scarce and little rainwater as well as the particles accompanied with the rainfall may have some effect on the carboxylic acids during the wintertime in Guiyang.Carboxylic acids in the rainwater of Guiyang were estimated to account for 18.7% to the free acidity, where formic, acetic and oxalic acids accounted for 7.9%, 4.7%, 6.1%, respectively.In Shangzhong, the 3 primary carboxylic acids represented 58.1% to the free acidity where formic, acetic and oxalic acids represented 25.1%, 7.5%, 25.5%, respectively.Carboxylic anions accounted for 6.6% (1.7%–19.2%) to the total anions in the rainwater of Guiyang.Carboxylic anions repre-sented 13.2% (0.5%–92.2%) to the total anions in the rainwater of Shangzhong.These results indicated that carboxylic acids were the important contributors to the rainwater acidity, especially in remote regions. | XU Gang LEE XinQing LU YingChun CHEN YingJun HUANG DaiKuan | 2010 | Chinese Science Bulletin2010，55，16： | 5 |
| 8 | Emission of intermediate volatility organic compounds from a ship main engine burning heavy fuel oil显示文摘Intermediate volatility organic compounds(IVOCs)are crucial precursors of secondary organic aerosol(SOA).In this study,gaseous IVOCs emitted from a ship main engine burning heavy fuel oil(HFO)were investigated on a test bench,which could simulate the real-world operations and emissions of ocean-going ships.The chemical compositions,emission factors(EFs)and volatility distributions of IVOC emissions were investigated.The results showed that the main engine burning HFO emitted a large amount of IVOCs,with average IVOC EFs of 20.2-201 mg/kg-fuel.The IVOCs were mainly comprised of unspeciated compounds.The chemical compositions of exhaust IVOCs were different from that of HFO fuel,especially for polycyclic aromatic compounds and alkylcyclohexanes.The volatility distributions of IVOCs were also different between HFO exhausts and HFO fuel.The distinctions in IVOC emission characteristics between HFO exhausts and HFO fuel should be considered when assessing the IVOC emission and related SOA formation potentials from ocean-going ships burning HFO,especially when using fuel-surrogate models. | Haijun Lou Yuejiao Hao Weiwei Zhang Penghao Su Fan Zhang Yingjun Chen Daolun Feng Yifan Li | 2019 | Journal of Environmental Sciences2019，31，10： | 5 |
| 9 | Fabrication of a hydroxyapatite-PDMS microfluidic chip for bone-related cell culture and drug screening显示文摘Bone is an important part of the human body structure and plays a vital role in human health.A microfluidic chip that can simulate the structure and function of bone will provide a platform for bone-related biomedical research.Hydroxyapatite(HA),a bioactive ceramic material,has a similar structure and composition to bone mineralization products.In this study,we used HA as a microfluidic chip component to provide a highly bionic bone environment.HA substrates with different microchannel structures were printed by using ceramic stereolithography(SLA)technology,and the minimum trench width was 50μm.The HA substrate with microchannels was sealed by a thin polydimethylsiloxane(PDMS)layer to make a HA-PDMS microfluidic chip.Cell culture experiments demonstrated that compared with PDMS,HA was more conducive to the proliferation and osteogenic differentiation of the human foetal osteoblast cell line(hFOB).In addition,the concentration gradient of the model drug doxorubicin hydrochloride(DOX)was successfully generated on a Christmas tree structure HA-PDMS chip,and the half maximal inhibitory concentration(IC50)of DOX was determined.The findings of this study indicate that the HA-PDMS microfluidic chip has great potential in the field of high-throughput bonerelated drug screening and bone-related research. | Qiangqiang Tang Xiaoyu Li Chen Lai Lei Li Hongkai Wu Yingjun Wang Xuetao Shi | 2021 | Bioactive Materials2021，6，1： | 5 |
| 10 | Genomic variants of genes associated with three horticultural traits in apple revealed by genome re-sequencing显示文摘The apple(Malus×domestica Borkh.)cultivar‘Su Shuai’exhibits greater disease resistance,shorter internodes and lighter fruit flavor compared with its parents‘Golden Delicious’and‘Indo’.To obtain a comprehensive overview of the sequence variation in these three horticultural traits,the genomes of‘Su Shuai’and‘Indo’were resequenced using next-generation sequencing and compared to the genome of‘Golden Delicious’.A wide range of genetic variations were detected,including 2454406 and 18749349 single nucleotide polymorphism(SNP)and 59547 and 50143 structural variants(SVs)in the‘Indo’and‘Su Shuai’genomes,respectively.Among the SVs in‘Su Shuai’,17 genes related to disease resistance,10 genes related to Gibberellin(GA)and 19 genes associated with fruit flavor were identified.The expression patterns of eight of the SV genes were examined using reverse transcription-quantitative polymerase chain reaction(RT-qPCR).The results of this study illustrate the genomic variation in these cultivars and provide evidence for a genetic basis for the horticultural traits of disease resistance,short internodes and lighter flavor exhibited in these cultivars.These results provide a genetic basis for the phenotypic characteristics of‘Su Shuai’and,as such,these SVs could serve as gene-specific molecular markers in maker-assisted breeding of apples. | Shijie Zhang Weiping Chen Lu Xin Zhihong Gao Yingjun Hou Xinyi Yu Zhen Zhang Shenchun Qu | 2014 | Horticulture Research2014，1，1： | 5 |
| 11 | Characterization of bioactive ceramic coatings prepared on titanium implants by micro-arc oxidation显示文摘微弧的氧化(毛) 是在电解质媒介的提高的化学工艺在阀门金属表面上获得涂层结构。毛在磷酸钠电解质获得的三氧化钛电影被调查。这些电影主要由在锐钛矿和金红石形式的 TiO_2 阶段组成并且在表面与 Na 和 P 元素充实。他们的导致磷灰石的能力在模仿的身体液体(SBF ) 被评估。在为超过 30 d 的 SBF 的 Whenimmersing,优先的 carbonated-hydroxyapatite 在这些电影的表面上被形成,它建议对待 MAO 的钛有有希望的积极生物回答。 | WANG Yingjun NAN Kaihui CHEN Xiaofeng NING Chengyun WANG lingyu ZHAO Naru | 2006 | Rare Metals2006，25，1： | 5 |
| 12 | Historical trends in the anthropogenic heavy metal levels in the tidal flat sediments of Lianyungang, China显示文摘The sedimentation of metals can preserve the historical record of contaminant input from local and regional sources and provide information on the historical changes in regionalwater and sediment quality. We report the 210Pb activities and the heavy metal (Cd, Cr, Cu, Mn, Pb and Zn)depth profiles from sediment cores retrieved in 2010. The mean sedimentation rates of 0.85-1.5 cm/yr aredetermined by 210 Pbdating. The sediments in the tidal flat have recorded heavy metaldeposition and thus allow the establishment of a connection between the temporal evolution of the heavy metal pollution and the historical changes in the economicdevelopment of Lianyungang. The enrichment factors (EF) are calculated to estimate the level of contamination stored in these sediments. The results show that in the studied sites, Cr and Cudisplay low EF values and are mainly from lithogenic origin. For the other studied trace metals, a great variability in the sedimentary record is observed. Significant anthropogenic enrichment over the last 50 years is revealed at the tidal flat that receives fluvial inputs. Zinc is the elementwith the highest EF values, followed by the order of Pb > Cd > Mn > Cu and Cr. The temporal variations of the heavy metals peakduring the late 1980s to the early2000s and show adecreasing trend afterward. The pollution intensity of the tidal flat isdetermined by using EF and thegeo-accumulation index (Igeo ),which show that, based on the Igeo scale, the tidal flat of Haizhou Bay is unpolluted to moderately polluted. | Rui Zhang Fan Zhang Yingjun Ding Jinrong Gao Jing Chen Li Zhou | 2013 | Journal of Environmental Sciences2013，25，7： | 5 |
| 13 | Raman micro-spectrometry as a technique for investigating heterogeneous reactions on individual atmospheric particles显示文摘Investigating the heterogeneous reaction on individual atmospheric particles is important because it approximates actual atmospheric conditions and can aid in reducing artifacts in elucidating real mechanisms and processes,and determining real kinetic parameters of the atmosphere relevance.This study developed a Raman microspectrometry method to investigate heterogeneous reactions on individual particles.The method was applied to the reaction of NO2 with individual CaCO3 particles.It was demonstrated that Raman microspectrometry can obtain information on both chemical composition and microscopic morphology at the same time,as well as information on chemical characteristics,such as the phase,which is useful for studying reaction processes.Raman spectra of individual particles deposited on a substrate showed no interference by morphological resonance,which is helpful for obtaining high-quality spectra.Moreover,Raman microspectrometry was compared with other methods for investigating heterogeneous reactions on individual particles and was found to have several advantageous characteristics. | ZHAO DeFeng,ZHU Tong,CHEN Qi,LIU YingJun & ZHANG ZeFeng State Key Joint Laboratory of Environmental Simulation and Pollution Control College of Environmental Sciences and Engineering,Peking University,Beijing 100871,China | 2011 | Science China Chemistry2011，54，1： | 5 |
| 14 | Signaling pathways in cancer-associated fibroblasts:recent advances and future perspectives显示文摘As a critical component of the tumor microenvironment(TME),cancerassociated fibroblasts(CAFs)play important roles in cancer initiation and progression.Well-known signaling pathways,including the transforming growth factor-β(TGF-β),Hedgehog(Hh),Notch,Wnt,Hippo,nuclear factor kappa-B(NF-κB),Janus kinase(JAK)/signal transducer and activator of transcription(STAT),mitogen-activated protein kinase(MAPK),and phosphoinositide 3-kinase(PI3K)/AKT pathways,as well as transcription factors,including hypoxia-inducible factor(HIF),heat shock transcription factor 1(HSF1),P53,Snail,and Twist,constitute complex regulatory networks in theTMEtomodulate the formation,activation,heterogeneity,metabolic characteristics and malignant phenotype of CAFs.Activated CAFs remodel the TME and influence the malignant biological processes of cancer cells by altering the transcriptional and secretory characteristics,and this modulation partially depends on the regulation of signaling cascades.The results of preclinical and clinical trials indicated that therapies targeting signaling pathways in CAFs demonstrated promising efficacy but were also accompanied by some failures(e.g.,NCT01130142 and NCT01064622).Hence,a comprehensive understanding of the signaling cascades in CAFs might help us better understand the roles of CAFs and the TME in cancer progression and may facilitate the development of more efficient and safer stroma-targeted cancer therapies.Here,we review recent advances in studies of signaling pathways in CAFs and briefly discuss some future perspectives on CAF research. | Zengli Fang Qingcai Meng Jin Xu Wei Wang Bo Zhang Jiang Liu Chen Liang Jie Hua Yingjun Zhao Xianjun Yu Si Shi | 2023 | Cancer Communications2023，43，1： | 5 |
| 15 | Black-box testing based on colorful taint analysis显示文摘Software vulnerability detection is one of the most important methods for guaranteeing software security.Two main classes of methods can detect vulnerabilities in binary files:white-box testing and black-box testing.The former needs to construct and solve path constraints to detect vulnerabilities.It has two main drawbacks:path exploding and complexity of constraints.The latter often aimlessly exhausts various inputs to test binary files.This paper combines both testing methods to detect vulnerabilities in binary files.By analyzing the input elements that aect check condition corresponding to a certain check point,we can generate one class of inputs that get to the check point to increase fuzzing eciency.By analyzing the relationship between guard conditions and check condition,the redundant check points are removed.Colorful taint analysis method(CTAM)is proposed to compute guard conditions,which is more ecient than traditional taint analysis method(TTAM).We implemented a prototype and made several experiments on it.The results showed that our method could increase the eciency of black-box testing. | CHEN Kai FENG DengGuo SU PuRui ZHANG YingJun | 2012 | Science China(Information Sciences)2012，55，1： | 3 |
| 16 | Application of Preoperative Visits during the Perioperative Period of Ophthalmic Surgery显示文摘Purpose:.To evaluate the effect of preoperative visits on patients′ psychology, physiology, and behavior during the perioperative period of eye surgery under local anesthesia, with the aim of enhancing patients′ cooperation with the surgery and improving their degree of satisfaction.Methods:.A total of 240 patients scheduled to undergo eye surgery between August and October 2013 were randomly divided into an observation(n = 120) and a control(n = 120)group..Patients in the observation group attended preoperative visits with nurses and received conventional nursing care. The control group received only conventional nursing.Results:.The Zung self-rating anxiety scale(SAS) scores were significantly lower in patients from the observation group than in the control group(P<0.05). Surgeons operating on the observation group were more satisfied with their patients′ cooperation with the surgery than were surgeons operating on the controls(P<0.01). Patients in the observation group had a significantly higher degree of satisfaction in terms of work efficiency in the operating room(P<0.01).Conclusion:.Preoperative visits by patients scheduled to undergo eye surgery can effectively mitigate preoperative anxiety in those patients,.build up a positive attitude toward the upcoming surgery,.instruct the patients to coordinate with the surgery,.enhance surgical safety,.and improve the patients′degree of satisfaction regarding the nursing care in the operating room. | Dan Wu Yingjun Chen Yanchun Wu Haihuan Li Miaoyu Fu Yanchan Liu | 2015 | Eye Science2015，30，2： | 3 |
| 17 | ONE-CELL-STATE METHOD FOR DETERMINATION OF ELECTRONIC STRUCTURE OF INTERMETALLIC COMPOUNDS显示文摘INTRODUCTIONInrecentyears,thePauling’svalencebond(VB)theoryhasbenmadeagreatprogresindesigninganddeterminingtheatomicstate,and... | Gao Yingjun , Chen Zhenhua , Huang Peiyun and Zhong Xiaping Institute of Nonequilibrium Materials, Central South University of Technology, Changsha 410083, P. R. China Physical Department, Guangxi University, Nanning 530004, P | 1998 | 中国有色金属学会会刊:英文版1998，8，4： | 2 |
| 18 | Fabrication and characterization of a PAM modified PHBV/BG scaffold显示文摘In order to improve the bioactivity and mechanical strength of the scaffold used in bone repair simultaneously, a novel porous PAM-poly (β-hydroxybutyrate-co-β-hydroxyvalerate) (PHBV)/bioactive glass (BG) scaffold was prepared by photo-initiated polymerization. PAM was used to improve the hydrophilicity of PHBV matrix while the BG particles were added to increase the bioactivity and strength of the matrix synchronously. The grafted amide group and Si-O moieties from acrylamide and the added BG were confirmed by Fourier Transform Infrared Spectrometry (FTIR). The micromorphology of the scaffolds before and after grafting was observed by scanning electron microscopy (SEM). The resulting images demonstrate that the PAM-PHBV/BG scaffold has a well connected pore structure and appropriate pore size which may be convenient for cells to grow and discharge metabolites. The specific gravity method was used to evaluate the pore property of the scaffold and the result shows that the scaffold has an average porosity up to 82.0%. Mercury intrusion porosimetry (MIP) indicated that the pores of PAM-PHBV/BG scaffold were mainly distributed between 75 and 150 μm. The compressive strength test was adopted to evaluate the mechanical property of the scaffold. The result shows that the PAM-PHBV/BG scaffold has a relatively high compressive strength (0.91 MPa) when compared with the pure PHBV scaffold. Besides, the properties of the pure PHBV scaffold, PHBV/BG scaffold were also evaluated. The newly prepared PAM-PHBV/BG scaffold may be worthy of further studying as a bone repair material. | WANG YingJun HUANG Wei REN Li CHEN XiaoFeng KE Yu | 2009 | Chinese Science Bulletin2009，54，17： | 2 |
| 19 | Surfactant-assisted synthesis of hydroxyapatite particles显示文摘 | YingJun Wang JingDi Chen Kun Wei ShuHua Zhang XiDong Wang | 2006 | Materials Letters2006，，27： | 2 |
| 20 | Whole-exome sequencing reveals twovariants in thegene in two Chinese patients with left ventricular non-compaction cardiomyopathy显示文摘Importance:Pathogenic variants in theRBM20 gene are associated with aggressive dilated cardiomyopathy(DCM).Recently,RBM20 was found to be associated with left ventricular non-compaction cardiomyopathy(LVNC).Thus far,only five families with LVNC have been reported to carry variants inRBM20.It remains unknown whether the variants inRBM20 associated with DCM can also cause LVNC.Objective:To elucidate the causativeRBM20 variant in two unrelated patients with both LVNC and DCM,and to identify the clinical characteristics associated with variants inRBM20.Methods:Trio whole-exome sequencing(WES)was performed.Variants were filtered and classified in accordance with the guidelines of the American College of Medical Genetics and Genomics(ACMG).Results:We identified two distinctde novo variants inRBM20(one per patient)in these two patients with LVNC.Both variants have been reported in patients with DCM,without the LVNC phenotype.Patient 1 was an 11-year-old girl who had DCM,LVNC,and heart failure;the ratio of noncompacted-to-compacted myocardium was 2.7:1.Ade novo heterozygous variant c.1907G>A(p.Arg636His)in exon 9 was identified in this patient.Patient 2 was a 13-year-old boy who had clinical phenotypes identical to those of Patient 1;the ratio of noncompacted-to-compacted myocardium was 3.2:1 in this patient.WES revealed ade novo heterozygous variant c.1909A>G(p.Ser637Gly)in exon 9.Both variants were previously characterized as pathogenic,and our study classified them as pathogenic variants based on the ACMG guidelines.Interpretation:We found that two patients with LVNC had variants inRBM20.Our results extended the clinical spectrum of the twoRBM20 variants and illustrated that the same variant inRBM20 can cause DCM,with or without the LVNC phenotype. | Qiqing Sun Jun Guo Chanjuan Hao Ruolan Guo Xuyun Hu Yuanying Chen Weili Yang Wei Li Yingjun Feng | 2020 | Pediatric Investigation2020，4，1： | 2 |
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